VIOLIN: Vaccine Investigation and Online Information Network

Mycobacterium tuberculosis

Table of Contents

Protective Antigens
1. Ag85A
2. Apa
3. ESAT-6
4. EsxA
5. EsxB
6. FbpA
7. FbpB from M. tuberculosis
8. FbpD
9. HBHA
10. KatG
11. Mpt63
12. MPT64
13. Mtb72F
14. PE20
15. PepA
16. PPE14
17. PPE18
18. PPE31
19. PstS3
20. SecA2

I. Vaccine Related Protective Antigens

1. Ag85A

Gene Name : Ag85A
VO ID : VO_0010936
Other Database IDs : LOCUS:AY207395.1;GI:29027586;GI:29027587
Taxonomy ID : 1773
Gene Strand (Orientation) : ?
Protein Note : T-cell immunogen and cytotoxic T lymphocyte immunogen.

DNA Sequence : Show Sequence

>gi|29027586|gb|AY207395.1| Mycobacterium tuberculosis secreted antigen Ag85A gene, complete cds
ATGCAGCTTGTTGACAGGGTTCGTGGCGCCGTCACGGGTATGTCGCGTCGACTCGTGGTCGGGGCCGTCG
GCGCGGCCCTAGTGTCGGGTCTGGTCGGCGCCGTCGGTGGCACGGCGACCGCGGGGGCATTTTCCCGCCC
GGGCTTGCCGGTGGAGTACCTGCAAGTGCCGTCGCCGTCGATGGGCCGTGACATCAAGGTCCAATTCCAA
AGTGGCGGTGCCAACTCGCCCGCCCTGTACCTGCTCGACGGCCTGCGCGCGCAGGACGACTTCAGCGGCT
GGGACATAAACACCCCGGCGTTCGAGTGGTACGACCAGTCGGGCCTGTCGGTGGTCATGCCGGTGGGTGG
CCAGTCAAGCTTCTACTCCGACTGGTACCAGCCCGCCTGCGGCAAGGCCGGTTGCCAGACTTACAAGTGG
GAGACCTTCCTGACCAGCGAGCTGCCGGGGTGGCTGCAGGCCAACAGGCACGTCAAGCCCACCGGAAGCG
CCGTCGTCGGTCTTTCGATGGCTGCTTCTTCGGCGCTGACGCTGGCGATCTATCACCCCCAGCAGTTCGT
CTACGCGGGAGCGATGTCGGGCCTGTTGGACCCCTCCCAGGCGATGGGTCCCACCCTGATCGGCCTGGCG
ATGGGTGACGCTGGCGGCTACAAGGCCTCCGACATGTGGGGCCCGAAGGAGGACCCGGCGTGGCAGCGCA
ACGACCCGCTGTTGAACGTCGGGAAGCTGATCGCCAACAACACCCGCGTCTGGGTGTACTGCGGCAACGG
CAAGCCGTCGGATCTGGGTGGCAACAACCTGCCGGCCAAGTTCCTCGAGGGCTTCGTGCGGACCAGCAAC
ATCAAGTTCCAAGACGCCTACAACGCCGGTGGCGGCCACAACGGCGTGTTCGACTTCCCGGACAGCGGTA
CGCACAGCTGGGAGTACTGGGGCGCGCAGCTCAACGCTATGAAGCCCGACCTGCAACGGGCACTGGGTGC
CACGCCCAACGCCGTGCCCGCGCCCCAGGGCGCCTAG

Protein Sequence : Show Sequence

>gi|29027587|gb|AAO62004.1| secreted antigen Ag85A [Mycobacterium tuberculosis]
MQLVDRVRGAVTGMSRRLVVGAVGAALVSGLVGAVGGTATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQ
SGGANSPALYLLDGLRAQDDFSGWDINTPAFEWYDQSGLSVVMPVGGQSSFYSDWYQPACGKAGCQTYKW
ETFLTSELPGWLQANRHVKPTGSAVVGLSMAASSALTLAIYHPQQFVYAGAMSGLLDPSQAMGPTLIGLA
MGDAGGYKASDMWGPKEDPAWQRNDPLLNVGKLIANNTRVWVYCGNGKPSDLGGNNLPAKFLEGFVRTSN
IKFQDAYNAGGGHNGVFDFPDSGTHSWEYWGAQLNAMKPDLQRALGATPNAVPAPQGA

Molecule Role : Protective antigen
Related Vaccine(s): Caspase-3 DNA Vaccine , rBCG-Ag85A[Tokyo]

2. Apa

Gene Name : Apa
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0011175
NCBI Gene ID : 885896
NCBI Protein GI : 57116926
Locus Tag : Rv1860
Genbank Accession : BX842578
Protein Accession : YP_177849
Taxonomy ID : 83332
Gene Starting Position : 2107735
Gene Ending Position : 2108712
Gene Strand (Orientation) : ?
Protein Name : hypothetical protein
Protein pI : 4.7
Protein Weight : 30636.65
Protein Length : 325
Protein Note : Rv1860, (MT1908, MTCY359.0013), len: 325 aa. apa (alternate gene names: mpt32, modD), Ala-, Pro-rich 45/47 kDa secreted protein, very similar to P46842|N43L_MYCLE from Mycobacterium leprae (287 aa), FASTA scores: opt: 1166, E(): 0, (66.4% identity in 298 aa overlap). Known to be glycosylated fibronectin-binding protein (see some citations). CHANGES IN THE MANNOSYLATION PATTERN OF THIS PROTEIN AFFECT ITS ABILITY TO STIMULATE T-LYMPHOCYTE RESPONSE. MAJOR IMMUNODOMINANT ANTIGEN THAT HAS POTENTIAL AS A VACCINE AGAINST TUBERCULOSIS. APA-ELISA COULD BE USED IN DIAGNOSIS. TBparse score is 0.924.; mpt32; modD

DNA Sequence : Show Sequence

>gi|57116681:2107735-2108712 Mycobacterium tuberculosis H37Rv, complete genome
CATGCATCAGGTGGACCCCAACTTGACACGTCGCAAGGGACGATTGGCGGCACTGGCTATCGCGGCGATG
GCCAGCGCCAGCCTGGTGACCGTTGCGGTGCCCGCGACCGCCAACGCCGATCCGGAGCCAGCGCCCCCGG
TACCCACAACGGCCGCCTCGCCGCCGTCGACCGCTGCAGCGCCACCCGCACCGGCGACACCTGTTGCCCC
CCCACCACCGGCCGCCGCCAACACGCCGAATGCCCAGCCGGGCGATCCCAACGCAGCACCTCCGCCGGCC
GACCCGAACGCACCGCCGCCACCTGTCATTGCCCCAAACGCACCCCAACCTGTCCGGATCGACAACCCGG
TTGGAGGATTCAGCTTCGCGCTGCCTGCTGGCTGGGTGGAGTCTGACGCCGCCCACTTCGACTACGGTTC
AGCACTCCTCAGCAAAACCACCGGGGACCCGCCATTTCCCGGACAGCCGCCGCCGGTGGCCAATGACACC
CGTATCGTGCTCGGCCGGCTAGACCAAAAGCTTTACGCCAGCGCCGAAGCCACCGACTCCAAGGCCGCGG
CCCGGTTGGGCTCGGACATGGGTGAGTTCTATATGCCCTACCCGGGCACCCGGATCAACCAGGAAACCGT
CTCGCTCGACGCCAACGGGGTGTCTGGAAGCGCGTCGTATTACGAAGTCAAGTTCAGCGATCCGAGTAAG
CCGAACGGCCAGATCTGGACGGGCGTAATCGGCTCGCCCGCGGCGAACGCACCGGACGCCGGGCCCCCTC
AGCGCTGGTTTGTGGTATGGCTCGGGACCGCCAACAACCCGGTGGACAAGGGCGCGGCCAAGGCGCTGGC
CGAATCGATCCGGCCTTTGGTCGCCCCGCCGCCGGCGCCGGCACCGGCTCCTGCAGAGCCCGCTCCGGCG
CCGGCGCCGGCCGGGGAAGTCGCTCCTACCCCGACGACACCGACACCGCAGCGGACCTTACCGGCCTG

Protein Sequence : Show Sequence

>gi|57116926|ref|YP_177849.1| hypothetical protein Rv1860 [Mycobacterium tuberculosis H37Rv]
MHQVDPNLTRRKGRLAALAIAAMASASLVTVAVPATANADPEPAPPVPTTAASPPSTAAAPPAPATPVAP
PPPAAANTPNAQPGDPNAAPPPADPNAPPPPVIAPNAPQPVRIDNPVGGFSFALPAGWVESDAAHFDYGS
ALLSKTTGDPPFPGQPPPVANDTRIVLGRLDQKLYASAEATDSKAAARLGSDMGEFYMPYPGTRINQETV
SLDANGVSGSASYYEVKFSDPSKPNGQIWTGVIGSPAANAPDAGPPQRWFVVWLGTANNPVDKGAAKALA
ESIRPLVAPPPAPAPAPAEPAPAPAPAGEVAPTPTTPTPQRTLPA

Molecule Role : Protective antigen
Molecule Role Annotation : We show that the 50- to 55-kDa Apa protein, specified by the Rv1860 gene of M. tuberculosis, can elicit both lymphoproliferative response and gamma interferon (IFN-gamma) production. A poxvirus recombinant expressing the Apa protein conferred a significant level of protective immunity in guinea pigs against a challenge dose of virulent M. tuberculosis (Kumar et al., 2003).
Related Vaccine(s): M. tuberculosis DNA Vaccine APADNA priming and APAMVA boosting

3. ESAT-6

Gene Name : ESAT-6
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 886209
NCBI Protein GI : 57117165
Locus Tag : Rv3875
Genbank Accession : FJ014499
Protein Accession : YP_178023
Taxonomy ID : 83332
Gene Starting Position : 4352608
Gene Ending Position : 4352895
Gene Strand (Orientation) : ?
Protein Name : 6 kDa early secretory antigenic target ESXA (ESAT-6)
Protein pI : 4.19
Protein Weight : 9605.81
Protein Length : 95
Protein Note : Rv3875, (MT3989, MTV027.10), len: 95 aa. esxA, early secretory antigenic target (see citations below), identical to Q57165|O84901|ESAT6 EARLY SECRETORY ANTIGENIC TARGET from Mycobacterium bovis (94 aa), FASTA scores: opt: 596, E(): 4.6e-33, (100.0% identity in 94 aa overlap). Also similar to Q50206|ESA6_MYCLE|ESAT6|ESX|L45|ML0049|MLCB628.12c 6 KDA EARLY SECRETORY ANTIGENIC TARGET HOMOLOG (ESAT-6-LIKE PROTEIN) (L-ESAT) from Mycobacterium leprae (95 aa), FASTA scores: opt: 236, E(): 3.3e-09, (36.25% identity in 91 aa overlap); and weak similarity with others proteins ESAT-like from Mycobacterium leprae. Also some similarity with O53266|ES69_MYCTU|Rv3019c|MT3104|MTV012.33c PUTATIVE SECRETED ESAT-6 LIKE PROTEIN 9 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 131, E(): 0.03, (26.15% identity in 88 aa overlap); and other ESAT-like protein. Contains probable coiled-coil from 56 to 92 aa. BELONGS TO THE ESAT6 FAMILY. Note that previously known as esat-6.; esat-6

DNA Sequence : Show Sequence

>gi|57116681:4352608-4352895 Mycobacterium tuberculosis H37Rv, complete genome
CATGACAGAGCAGCAGTGGAATTTCGCGGGTATCGAGGCCGCGGCAAGCGCAATCCAGGGAAATGTCACG
TCCATTCATTCCCTCCTTGACGAGGGGAAGCAGTCCCTGACCAAGCTCGCAGCGGCCTGGGGCGGTAGCG
GTTCGGAGGCGTACCAGGGTGTCCAGCAAAAATGGGACGCCACGGCTACCGAGCTGAACAACGCGCTGCA
GAACCTGGCGCGGACGATCAGCGAAGCCGGTCAGGCAATGGCTTCGACCGAAGGCAACGTCACTGGGATG
TTCGCATA

Protein Sequence : Show Sequence

>gi|57117165|ref|YP_178023.1| 6 kDa early secretory antigenic target ESXA (ESAT-6) [Mycobacterium tuberculosis H37Rv]
MTEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQ
NLARTISEAGQAMASTEGNVTGMFA

Molecule Role : Protective antigen
Molecule Role Annotation : In a prophylactic vaccine model, immunization with the Mycobacterium tuberculosis protein ESAT-6 with LANAC adjuvant elicited significant protective immunity against aerosol challenge with virulent M. tuberculosis (Zaks et al., 2006).

4. EsxA

Gene Name : EsxA
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010919
NCBI Gene ID : 886209
NCBI Protein GI : 57117165
Locus Tag : Rv3875
Genbank Accession : NC_000962.2
Protein Accession : NC_000962.2
Other Database IDs : EMBL: AE000516
EMBL: AF004671
EMBL: AF420491
EMBL: BX842584
EMBL: X79562
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
InterPro: IPR010310
KEGG: mtc:MT3989
KEGG: mtu:Rv3875
PDB: 1WA8
Pfam: PF06013
PIR: A70803
TIGR: MT3989
TubercuList: Rv3875
UniProt: P0A564
Taxonomy ID : 1773
Gene Starting Position : 4352609
Gene Ending Position : 4352896
Gene Strand (Orientation) : +
Protein Name : 6 KDA EARLY SECRETORY ANTIGENIC TARGET ESXA (ESAT-6)
Protein pI : 4.19
Protein Weight : 9903.8
Protein Length : 95
Protein Note : Rv3875, (MT3989, MTV027.10), len: 95 aa. esxA, early secretory antigenic target (see citations below), identical to Q57165|O84901|ESAT6 EARLY SECRETORY ANTIGENIC TARGET from Mycobacterium bovis (94 aa), FASTA scores: opt: 596, E(): 4.6e-33, (100.0% identity in 94 aa overlap). Also similar to Q50206|ESA6_MYCLE|ESAT6|ESX|L45|ML0049|MLCB628.12c 6 KDA EARLY SECRETORY ANTIGENIC TARGET HOMOLOG (ESAT-6-LIKE PROTEIN) (L-ESAT) from Mycobacterium leprae (95 aa), FASTA scores: opt: 236, E(): 3.3e-09, (36.25% identity in 91 aa overlap); and weak similarity with others proteins ESAT-like from Mycobacterium leprae. Also some similarity with O53266|ES69_MYCTU|Rv3019c|MT3104|MTV012.33c PUTATIVE SECRETED ESAT-6 LIKE PROTEIN 9 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 131, E(): 0.03, (26.15% identity in 88 aa overlap); and other ESAT-like protein. Contains probable coiled-coil from 56 to 92 aa. BELONGS TO THE ESAT6 FAMILY. Note that previously known as esat-6.; esat-6
Protein Annotation : FUNCTION: Not known. Elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response (UniProt: P0A564)

SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A564)

SIMILARITY: Belongs to the ESAT-6 (esx) family (UniProt: P0A564)

DNA Sequence : Show Sequence

>GeneID|886209 [Mycobacterium tuberculosis H37Rv ] 4352609..4352896
atgacagagcagcagtggaatttcgcgggtatcgaggccgcggcaagcgcaatccagggaaatgtcacgt
ccattcattccctccttgacgaggggaagcagtccctgaccaagctcgcagcggcctggggcggtagcgg
ttcggaggcgtaccagggtgtccagcaaaaatgggacgccacggctaccgagctgaacaacgcgctgcag
aacctggcgcggacgatcagcgaagccggtcaggcaatggcttcgaccgaaggcaacgtcactgggatgt
tcgcatag

Protein Sequence : Show Sequence

>gi|57117165|ref|YP_178023.1| 6 KDA EARLY SECRETORY ANTIGENIC TARGET ESXA (ESAT-6) [Mycobacterium tuberculosis H37Rv ]
MTEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQ
NLARTISEAGQAMASTEGNVTGMFA

Molecule Role : Protective antigen
Molecule Role Annotation : The heterologous prime-boost strategy of live Salmonella vaccine expressing Mycobacterium tuberculosis fusion antigen Age5B-ESAT6 (esxA) from the chromosome followed by a systemic boost of antigen and adjuvant reduced the levels of M. tuberculosis bacteria in the lungs and spleen to the same extent as BCG. Additionally, this vaccination regimen was observed to be statistically equivalent in terms of protection to immunisation with BCG (Hall et al., 2009).
Related Vaccine(s): Ag85B and ESAT-6 fusion protein , attenuated M. bovis strain WAg539 , recombinant S. typhimurium secreting M. tuberculosis ESAT-6

5. EsxB

Gene Name : EsxB
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010951
NCBI Gene ID : 886194
NCBI Protein GI : 15611010
Locus Tag : Rv3874
Genbank Accession : BX842584
Protein Accession : NP_218391
Other Database IDs : EMBL: AE000516
EMBL: AF004671
EMBL: AF419854
EMBL: BX842584
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
InterPro: IPR010310
KEGG: mtc:MT3988
KEGG: mtu:Rv3874
Pfam: PF06013
PIR: H70802
SMR: P0A566
TIGR: MT3988
TubercuList: Rv3874
UniProt: P0A566
Taxonomy ID : 83332
Gene Starting Position : 4352273
Gene Ending Position : 4352575
Gene Strand (Orientation) : +
Protein Name : 10 kDa culture filtrate antigen EsxB
Protein pI : 4.31
Protein Weight : 10396.66
Protein Length : 100
Protein Note : Rv3874, (MT3988, MTV027.09), len: 100 aa. esxB, 10 KDA culture filtrate antigen (see citations below, especially first), highly similar to O33084|CF10_MYCLE|ML0050|MLCB628.13c 10 KDA CULTURE FILTRATE ANTIGEN CFP10 HOMOLOG from Mycobacterium leprae (99 aa), FASTA scores: opt: 237, E(): 2.4e-08, (39.4% identity in 99 aa overlap). Also similar to O05440|ES6D_MYCTU|Rv3905c|MT4024|MTCY15F10.06 PUTATIVE ESAT-6 LIKE PROTEIN 13 from Mycobacterium tuberculosis (103 aa) FASTA scores: opt: 126, E(): 0.18, (23.1% identity in 91 aa overlap); and shows some similarity with other proteins from Mycobacterium tuberculosis. Contains probable coiled-coil from aa 49-93. BELONGS TO THE ESAT6 FAMILY. Note that previously known as lhp (alternate gene name: cfp10).; lhp, cfp10
Protein Annotation : SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A566)

SIMILARITY: Belongs to the ESAT-6 (esx) family (UniProt: P0A566)

DNA Sequence : Show Sequence

>gi|57116681:4352273-4352575 Mycobacterium tuberculosis H37Rv, complete genome
CATGGCAGAGATGAAGACCGATGCCGCTACCCTCGCGCAGGAGGCAGGTAATTTCGAGCGGATCTCCGGC
GACCTGAAAACCCAGATCGACCAGGTGGAGTCGACGGCAGGTTCGTTGCAGGGCCAGTGGCGCGGCGCGG
CGGGGACGGCCGCCCAGGCCGCGGTGGTGCGCTTCCAAGAAGCAGCCAATAAGCAGAAGCAGGAACTCGA
CGAGATCTCGACGAATATTCGTCAGGCCGGCGTCCAATACTCGAGGGCCGACGAGGAGCAGCAGCAGGCG
CTGTCCTCGCAAATGGGCTTCTG

Protein Sequence : Show Sequence

>gi|15611010|ref|NP_218391.1| 10 kDa culture filtrate antigen EsxB [Mycobacterium tuberculosis H37Rv]
MAEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELD
EISTNIRQAGVQYSRADEEQQQALSSQMGF

Molecule Role : Protective antigen
Molecule Role Annotation : The 10-kDa culture filtrate protein (CFP-10, esxB) and 6-kDa early secretory antigen of T cells (ESAT-6) are secreted in abundance by Mycobacterium tuberculosis. Study demonstrated that protective immunity is induced by CFP-10 DNA vaccination in C3H mice as measured by a CFU reduction in the lung and spleen 4 and 8 weeks after challenge with M. tuberculosis (Wu et al., 2008).
Related Vaccine(s): attenuated M. bovis strain WAg539

6. FbpA

Gene Name : FbpA
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010952
NCBI Gene ID : 886132
NCBI Protein GI : 15610940
Locus Tag : Rv3804c
Genbank Accession : NC_000962.2
Protein Accession : NC_000962.2
Other Database IDs : EMBL: AE000516
EMBL: BX842584
EMBL: M27016
EMBL: U47335
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
InterPro: IPR000801
KEGG: mtc:MT3911
KEGG: mtu:Rv3804c
PDB: 1SFR
Pfam: PF00756
PIR: H70887
TIGR: MT3911
TubercuList: Rv3804c
UniProt: P0A4V2
Taxonomy ID : 1773
Gene Starting Position : 4265642
Gene Ending Position : 4266658
Gene Strand (Orientation) : -
Protein Name : SECRETED ANTIGEN 85-A FBPA (MYCOLYL TRANSFERASE 85A) (FIBRONECTIN-BINDING PROTEIN A) (ANTIGEN 85 COMPLEX A)
Protein pI : 6.51
Protein Weight : 35686
Protein Length : 338
Protein Note : Rv3804c, (MT3911, MTV026.09c), len: 338 aa. fbpA (alternate gene names: mpt44, 85A), precursor of the 85-A antigen (fibronectin-binding protein A) (mycolyl transferase 85A) (EC 2.3.1.-) (see citations below), identical to P17944|P17996|FBPA|MPT44 ANTIGEN 85-A PRECURSOR from Mycobacterium bovis (338 aa), FASTA scores: opt: 2341, E(): 1.2e-132, (100.0% identity in 338 aa overlap); and highly similar to other Mycobacterial antigen precursors e.g. O52956|A85A_MYCAV|FBPA ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium avium (347 aa), FASTA scores: opt: 1987, E(): 1.7e-111, (82.55% identity in 338 aa overlap); Q05861|A85A_MYCLE|FBPA|ML0097 ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium leprae (330 aa), FASTA scores: opt: 1936, E(): 1.9e-108, (83.0% identity in 329 aa overlap); O06052|A85A_MYCGO|FBPA ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium gordonae (339 aa), FASTA scores: opt: 1932, E(): 3.3e-108, (80.45% identity in 338 aa overlap); etc. Also highly similar to P31952|A85B_MYCTU|FBPB|Rv1886c|MT1934|MTCY180.32 SECRETED ANTIGEN 85-B from Mycobacterium tuberculosis (325 aa), FASTA scores: opt: 1830, E(): 3.9e-102, (78.85% identity in 317 aa overlap); P31953|A85C_MYCTU|FBPC|MPT45|Rv0129c|MTCI5.03c|FBPC2 SECRETED ANTIGEN 85-C from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 1597, E(): 3.4e-88, (67.25% identity in 336 aa overlap).; mpt44; 85A
Protein Annotation : FUNCTION: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity (UniProt: P0A4V2)

SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A4V2)

PTM: Contains one disulfide bridge (UniProt: P0A4V2)

SIMILARITY: Belongs to the mycobacterial A85 antigen family (UniProt: P0A4V2)

DNA Sequence : Show Sequence

>GeneID|886132 [Mycobacterium tuberculosis H37Rv ] 4265642..4266658
ctaggcgccctggggcgcgggcccggtgttgggcgtggcacccagtgcccgttgcaggtcgggcttcata
gcgttgagctgcgcgccccagtactcccagctgtgcgtaccgctgtccgggaagtcgaacacgccgttgt
ggccgccaccggcgttgtaggcgtcttggaacttgatgttgctggtccgcacgaagccctcgaggaactt
ggccggcaggttgttgccacccagatccgacggcttgccgttgccgcagtacacccagacgcgggtgttg
ttggcgatcagcttcccgacgttcaacagcgggtcgttgcgctgccacgccgggtcctccttcgggcccc
acatgtcggaggccttgtagccgccagcgtcacccatcgccaggccgatcagggtgggacccatcgcctg
ggaggggtccaacaggcccgacatcgctcccgcgtagacgaactgctgggggtgatagatcgccagcgtc
agcgccgaagaagcagccatcgaaagaccgacgacggcgcttccggtgggcttgacgtgcctgttggcct
gcagccaccccggcagctcgctggtcaggaaggtctcccacttgtaagtctggcaaccggccttgccgca
ggcgggctggtaccagtcggagtagaagcttgactggccacccaccggcatgaccaccgacaggcccgac
tggtcgtaccactcgaacgccggggtgttgatgtcccagccgctgaagtcgtcctgcgcgcgcaggccgt
cgagcaggtacagggcgggcgagttggcaccaccactttggaattggaccttgatgtcacggcccatcga
cggcgacggcacctgcaggtactccaccggcaagcccggccgggaaaatgcccccgcggtcgccgtgcca
ccgacggcgccgaccagacccgacactagggccgcgccgacggccccgaccacgagtcgacgcgacatac
ccgtgacggcgccacgaaccctgtcaacaagctgcat

Protein Sequence : Show Sequence

>gi|15610940|ref|NP_218321.1| SECRETED ANTIGEN 85-A FBPA (MYCOLYL TRANSFERASE 85A) (FIBRONECTIN-BINDING PROTEIN A) (ANTIGEN 85 COMPLEX A) [Mycobacterium tuberculosis H37Rv ]
MQLVDRVRGAVTGMSRRLVVGAVGAALVSGLVGAVGGTATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQ
SGGANSPALYLLDGLRAQDDFSGWDINTPAFEWYDQSGLSVVMPVGGQSSFYSDWYQPACGKAGCQTYKW
ETFLTSELPGWLQANRHVKPTGSAVVGLSMAASSALTLAIYHPQQFVYAGAMSGLLDPSQAMGPTLIGLA
MGDAGGYKASDMWGPKEDPAWQRNDPLLNVGKLIANNTRVWVYCGNGKPSDLGGNNLPAKFLEGFVRTSN
IKFQDAYNAGGGHNGVFDFPDSGTHSWEYWGAQLNAMKPDLQRALGATPNTGPAPQGA

Molecule Role : Protective antigen
Molecule Role Annotation : Researchers have developed DNA vaccines expressing M. tuberculosis antigen 85A (Ag85A, fbpA) and cytokine granulocyte macrophage colony stimulating factor (GM-CSF). The immune strategy of BCG-prime and DNA vaccine boost induced mice to generate efficient immune protection against M. tuberculosis challenge (Dou et al., 2010).
Related Vaccine(s): Recombinant BCG, Ag85A in multiple vectors

7. FbpB from M. tuberculosis

Gene Name : FbpB from M. tuberculosis
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0011172
NCBI Gene ID : 885785
NCBI Protein GI : 15609023
Locus Tag : Rv1886c
Genbank Accession : BX842578
Protein Accession : NP_216402
Taxonomy ID : 83332
Gene Starting Position : 2134889
Gene Ending Position : 2135866
Gene Strand (Orientation) : -
Protein Name : secreted antigen 85-B fbpB (85B) (antigen 85 complex B) (Mycolyl transferase 85B) (fibronectin-binding protein B) (extracellular alpha-antigen)
Protein pI : 5.72
Protein Weight : 33091.46
Protein Length : 325
Protein Note : Rv1886c, (MT1934, MTCY180.32), len: 325 aa. fbpB (alternate gene names: mpt59, 85B), precursor of the 85-B antigen (fibronectin-binding protein B) (mycolyl transferase 85B) (EC 2.3.1.-) (see citations below), highly similar to other Mycobacterial antigen precursors e.g. P12942|A85B_MYCBO ANTIGEN 85-B PRECURSOR from Mycobacterium bovis (323 aa); P21160|A85B_MYCKA ANTIGEN 85-B PRECURSOR from Mycobacterium kansasii (325 aa); etc. Also highly similar to Mycobacterium tuberculosis antigen precursors: Rv3804c|fbpA (338 aa), Rv0129c|fbpC2 (340 aa), and Rv3803c|fbpC1 (299 aa). TBparse score is 0.912.; mpt59; 85B

DNA Sequence : Show Sequence

>gi|57116681:2134889-2135866 Mycobacterium tuberculosis H37Rv, complete genome
GTCAGCCGGCGCCTAACGAACTCTGCAGGTCACCCTTCATGGCGTTGAGCTGAGCGCCCCAGTACTCCCA
GCTGTGCGTGCCGTTGGGCGGGAAGTTGAACACGGCGTTGTGCCCGCCCGCGGCGTTGTACGCATCCTGG
AACTTCAGGTTGCTGCTACGAACGAAGTTCTCCAAGAACTCGGCGGGTATGTTGGCACCGCCCAACTCGT
TCGGGGTGCCGTTCCCGCAATAAACCCATAGCCGGGTGTTGTTTGCGACCAGCTTGGGGATCTGCTGCGT
AGGGTCGTTGCGCTCCCATGCCGGGTCACTCGAGGGACCCCACATGTCTGCGGCCTTGTAACCGCCGGCG
TCACCCATCGCGAGGCCGATCAGGCTAGGCCCCATCCCCTGAGAGGGGTCCAGCAGGGCCGACAGCGAGC
CGGCGTAGATGAACTGCTGGGGGTGGTAGGCGGCCAAGATCATTGCCGACGAGCCGGCCATCGACAAGCC
GATTGCAGCGCTGCCGGTGGGCTTCACGGCCCTGTTGGCGGACAACCATTGCGGCAGCTCGCTGGTCAGG
AAGGTTTCCCACTTGTAAGTCTGGCAGCCAGCCTTACCGCAGGCCGGGCTGTACCAGTCGCTGTAGAAGC
TGGACTGCCCGCCGACCGGCATGACTATCGACAGTCCCGACTGGTAGTACCACTCGAACGCCGGGGTGTT
GATATCCCAGCCGTTGTAGTCGTCTTGGGCGCGCAGGCCGTCGAGCAGATAAACCGCAGGTGAGTTGTTC
CCACCGCTCTGGAACTGAACCTTGATGTCGCGGCCCATCGACGGCGACGGCACCTGCAGGTACTCGACCG
GCAGCCCCGGCCGGGAGAACGCGCCCGCGGTTGCCGCTCCGCCGGCAAGCCCCACCAGGCCCGGAAGGAC
TACAGCCGCTGCCGTGCCGATCATCAATCGGCGTCCCCAAGCTCGAATCTTTCGGCTCACGTCTGTCA

Protein Sequence : Show Sequence

>gi|15609023|ref|NP_216402.1| secreted antigen 85-B fbpB (85B) (antigen 85 complex B) (Mycolyl transferase 85B) (fibronectin-binding protein B) (extracellular alpha-antigen) [Mycobacterium tuberculosis H37Rv]
MTDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQSGG
NNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETF
LTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGD
AGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKF
QDAYNAAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGAG

Molecule Role : Protective antigen
Molecule Role Annotation : Immunization with DNA encoding Ag85B (fbpB), a candidate vaccine antigen of Mycobacterium tuberculosis, elicited in mice a low but protective CD4(+) T cell-mediated IFN-gamma response (Palma et al., 2010).
Related Vaccine(s): M. tuberculosis DNA Vaccine encoding Ag85B Protein

8. FbpD

Gene Name : FbpD
Sequence Strain (Speices/Organism) : Mycobacterium bovis BCG str. Tokyo 172
NCBI Gene ID : 7562680
NCBI Protein GI : 224992215
Locus Tag : JTY_3867
Genbank Accession : AP010918
Protein Accession : YP_002646905
Taxonomy ID : 561275
Gene Starting Position : 4234158
Gene Ending Position : 4235057
Gene Strand (Orientation) : -
Protein Name : secreted MPT51/MPB51 antigen protein
Protein pI : 6.63
Protein Weight : 29500.66
Protein Length : 299

DNA Sequence : Show Sequence

>gi|224988383:4234158-4235057 Mycobacterium bovis BCG str. Tokyo 172, complete genome
CTTAGCGGATCGCACCGACGATATCGCCCGACATAGCGCCCAGCTGGGGCGCCCACGAGCCCCAGCCGTT
GTCACCGCTGGCTGGGAAGTCGAAGTGTCCGTTGTGCCCGCCGACGCTGCGATACTGGTTGTAGAACATG
CGGCTGTTACCCATCGCCTCGGCGGCTTGGCCGATCATGGCGGCGGGATCGCTGGCTCCCGGGTTGGTCG
GGCTCCACACCCACACCCGGGTGTTGTTTTGCGCCAGCAGGCTGGCATGCACCCACGGGTCGTGCCACTT
CCACCGACCCAGCTGTGGTGCTCCCCACATTCCGTTGGTGTCCACACCGCCGAATTGCTGCATGCCCGCC
GCGATCGCACCGTTGGTGGTGGTGTTCGACGGGTACAAAAAGCCCGACATCGAGCCAGCGAAGCCGAAGC
GGTCGGGGTGGAAGGCCGCCAGCGCCATCGCCCCGTAACCGCCCTGAGCGGCGCCAACGGCCGCATGGCC
ACCGGGGGCCAAGCCCCGGTTAGCGGCCAGCCAGTCGGGCAGCTCAGCGGACAAGAAGGTGTCCCACTGC
TTGCTGCCATCCTGCTCCCAGTTGGTGTACATGCTGTACGCACCACCGGCCGGTGCCACCACCGAAATCC
CCTTGCCCGCCAACGTGTTCATCGCGTTACCCGCGGTGACCCAGTTACTGACATCCGGGCCGGCGTTGAA
GGCGTCCAGCAGATACACCGCGTGCGGCCCACCGGCTAGGAAGGCCACCGGGATGTCCCGGCCCATCGAG
GGCGACGGCACCATCAGGTTCTCGTATGGGGCGGCCTTGGCGGTGGGTTCCGCGGCTACCGCGACACCGC
CCAACCCGAATGACAGTGCGGCAATCCAGAGCGCCCGCAGCAGCGCCGACCGACCCTTCA

Protein Sequence : Show Sequence

>gi|224992215|ref|YP_002646905.1| secreted MPT51/MPB51 antigen protein [Mycobacterium bovis BCG str. Tokyo 172]
MKGRSALLRALWIAALSFGLGGVAVAAEPTAKAAPYENLMVPSPSMGRDIPVAFLAGGPHAVYLLDAFNA
GPDVSNWVTAGNAMNTLAGKGISVVAPAGGAYSMYTNWEQDGSKQWDTFLSAELPDWLAANRGLAPGGHA
AVGAAQGGYGAMALAAFHPDRFGFAGSMSGFLYPSNTTTNGAIAAGMQQFGGVDTNGMWGAPQLGRWKWH
DPWVHASLLAQNNTRVWVWSPTNPGASDPAAMIGQAAEAMGNSRMFYNQYRSVGGHNGHFDFPASGDNGW
GSWAPQLGAMSGDIVGAIR

Molecule Role : Protective antigen
Molecule Role Annotation : Intravenous vaccination with recombinant bacteria carrying expression plasmid for MPB/MPT51 in BALB/c mice elicited significant protective responses, comparable to those evoked by a live Mycobacterium bovis BCG vaccine (Miki et al., 2004).

9. HBHA

Gene Name : HBHA
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Ra
NCBI Gene ID : 5211814
NCBI Protein GI : 148660242
Locus Tag : MRA_0482
Genbank Accession : CP000611
Protein Accession : YP_001281765
Taxonomy ID : 25177
Gene Starting Position : 567106
Gene Ending Position : 567705
Gene Strand (Orientation) : +
Protein Name : iron-regulated heparin binding hemagglutinin Hbha
Protein pI : 9.84
Protein Weight : 20541.79
Protein Length : 199

DNA Sequence : Show Sequence

>gi|148659757:567106-567705 Mycobacterium tuberculosis H37Ra, complete genome
CATGGCTGAAAACTCGAACATTGATGACATCAAGGCTCCGTTGCTTGCCGCGCTTGGAGCGGCCGACCTG
GCCTTGGCCACTGTCAACGAGTTGATCACGAACCTGCGTGAGCGTGCGGAGGAGACTCGTACGGACACCC
GCAGCCGGGTCGAGGAGAGCCGTGCTCGCCTGACCAAGCTGCAGGAAGATCTGCCCGAGCAGCTCACCGA
GCTGCGTGAGAAGTTCACCGCCGAGGAGCTGCGTAAGGCCGCCGAGGGCTACCTCGAGGCCGCGACTAGC
CGGTACAACGAGCTGGTCGAGCGCGGTGAGGCCGCTCTAGAGCGGCTGCGCAGCCAGCAGAGCTTCGAGG
AAGTGTCGGCGCGCGCCGAAGGCTACGTGGACCAGGCGGTGGAGTTGACCCAGGAGGCGTTGGGTACGGT
CGCATCGCAGACCCGCGCGGTCGGTGAGCGTGCCGCCAAGCTGGTCGGCATCGAGCTGCCTAAGAAGGCT
GCTCCGGCCAAGAAGGCCGCTCCGGCCAAGAAGGCCGCTCCGGCCAAGAAGGCGGCGGCCAAGAAGGCGC
CCGCGAAGAAGGCGGCGGCCAAGAAGGTCACCCAGAAGTA

Protein Sequence : Show Sequence

>gi|148660242|ref|YP_001281765.1| iron-regulated heparin binding hemagglutinin Hbha [Mycobacterium tuberculosis H37Ra]
MAENSNIDDIKAPLLAALGAADLALATVNELITNLRERAEETRTDTRSRVEESRARLTKLQEDLPEQLTE
LREKFTAEELRKAAEGYLEAATSRYNELVERGEAALERLRSQQSFEEVSARAEGYVDQAVELTQEALGTV
ASQTRAVGERAAKLVGIELPKKAAPAKKAAPAKKAAPAKKAAAKKAPAKKAAAKKVTQK

Molecule Role : Protective antigen
Molecule Role Annotation : Purified preparations of HBHA were emulsified in a dimethyldioctadecylammonium bromide-monophosphoryl lipid A adjuvant and tested for the ability to reduce M. tuberculosis infection in the mouse aerosol challenge model for tuberculosis. The HBHA-containing vaccine gave a approximately 0.7-log reduction in CFU in both mouse lungs and spleens compared to adjuvant controls 28 days following challenge (Parra et al., 2004).

10. KatG

Gene Name : KatG
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 885638
NCBI Protein GI : 15609045
Locus Tag : Rv1908c
Genbank Accession : BX842578
Protein Accession : NP_216424
Taxonomy ID : 83332
Gene Starting Position : 2153888
Gene Ending Position : 2156110
Gene Strand (Orientation) : -
Protein Name : catalase-peroxidase-peroxynitritase T KATG
Protein pI : 4.87
Protein Weight : 75544.06
Protein Length : 740
Protein Note : Rv1908c, (MTCY180.10), len: 740 aa. katG, catalase-peroxidase-peroxynitritase T (EC 1.11.1.6) (see citations below), HPI. FASTA results: Q57215 CATALASE-PEROXIDASE from Mycobacterium tuberculosis (740 aa) opt: 5081, E(): 0, (100% identity in 740 aa overlap). Contains peroxidases active site signature (PS00436) and ATP/GTP-binding site motif A (P-loop; PS00017). Cosmid sequence was corrected to agree with a sequencing read from the H37Rv genome. DELETIONS OR DEFECTS IN KATG GENE CAUSE ISONIAZID (INH) RESISTANCE. BELONGS TO THE PEROXIDASE FAMILY. BACTERIAL PEROXIDASE/CATALASE SUBFAMILY. KATG TRANSCRIPTION SEEMS TO BE REGULATED BY FURA|Rv1909c PRODUCT. The catalase-peroxidase activity is associated with the amino-terminal domain but no definite function has been assigned to the carboxy-terminal domain. TBparse score is 0.923.

DNA Sequence : Show Sequence

>gi|57116681:2153888-2156110 Mycobacterium tuberculosis H37Rv, complete genome
ATCAGCGCACGTCGAACCTGTCGAGGTTCATCACCTTGTCCCAGGCAGCGACGAAGTCCTGCACGAACTT
CGGCTGCGCGTCATCGGCGCCATAGACCTCGACAAGCGCCCGCAACTCCGAGTTGGACCCGAAGACCAGG
TCCACGCGGCTGCCGGTCCACTTCACCTTGCCACTGCCATCCTTGCCCTGGTAGGTCCCGTCATCTGCTG
GCGAGGGCTCCCAGGTGATACCCATGTCGAGCAGGTTCACGAAGAAGTCGTTGGTCAGTGACTCGGAGGC
CTCGGTGAACACGCCCAGCGGTAAGCGCTTGTAGTTTGCGCCGAGGACGCGCAGGCCACCTACCAGCACC
GTCATCTCAGGGGCACTGAGCGTAAGCAGGTTCGCCTTGTCGAGCAGCATGTACTCGGCCGGCAACGGGT
TGCCCTTTCCGAGGTAGTTTCGGAAGCCATCTGCCTTGGGCTCCAGCACGGCAAAGGATTCCACGTCGGT
TTGTTCCTGCGACGCATCCGTGCGGCCCGGGGTGAAGGGCACCGTGATGTTGTGGCCAGCCGCCTTTGCT
GCTTTCTCTATGGCGGCACAGCCACCGAGCACGACGAGGTCGGCGAAGGACACTTTGATGTTCCCCGGCG
CCGCGGAGTTGAATGACTCCTGGATCTCTTCCAGGGTGCGAATGACCTTGCGCAGATCCCCGTCGGGGTC
GTTGACCTCCCACCCGACTTGTGGCTGCAGGCGGATGCGACCACCGTTGGCGCCGCCGCGCTTGTCGCTA
CCACGGAACGACGACGCCGCCGCCCATGCGGTCGAAACTAGCTGTGAGACAGTCAATCCCGATGCCCGGA
TCTGGCTCTTAAGGCTGGCAATCTCGGCTTCGCCGACGAGGTCGTGGCTGACCGCAGGGACCGGATCCTG
CCACAGCAGGGTCTGCTTGGGGACCAGCGGCCCAAGGTATCTCGCAACGGGACCCATGTCTCGGTGGATC
AGCTTGTACCAGGCCTTGGCGAACTCGTCGGCCAATTCCTCGGGGTGTTCCAGCCAGCGACGCGTGATCC
GCTCATAGATCGGATCCACCCGCAGCGAGAGGTCAGTGGCCAGCATCGTCGGGGAGCGCCCTGGCCCGCC
GAACGGGTCCGGGATGGTGCCGGCACCGGCGCCGTCCTTGGCGGTGTATTGCCAAGCGCCAGCAGGGCTC
TTCGTCAGCTCCCACTCGTAGCCGTACAGGATCTCGAGGAAACTGTTGTCCCATTTCGTCGGGGTGTTCG
TCCATACGACCTCGATGCCGCTGGTGATCGCGTCCTTACCGGTTCCGGTGCCATACGAGCTCTTCCAGCC
CAAGCCCATCTGCTCCAGCGGAGCAGCCTCGGGTTCGGGGCCGACCAGATCGGCCGGGCCGGCGCCATGG
GTCTTACCGAAAGTGTGACCGCCGACGATCAGCGCCGCTGTTTCGACGTCGTTCATGGCCATGCGCCGAA
ACGTCTCGCGAATGTCGACCGCCGCGGCCATGGGGTCCGGGTTGCCGTTCGGCCCCTCCGGGTTCACGTA
GATCAGCCCCATCTGCACCGCGGCCAGCGGGTTCTCCAGATCCCGCTTACCGCTGTAACGCTCATCGCCG
AGCCAGGTGGCTTCCTTGCCCCAATAGACCTCATCGGGCTCCCACTGGTCGACCCGGCCGAAGCCGAACC
CGAACGTCTTGAAGCCCATCGATTCCAGCGCGCAGTTGCCGGCGAAAACAATCAGGTCCGCCCATGAGAG
CTTCTTGCCGTACTTCTTCTTGACCGGCCACAGCAGCCGGCGCGCCTTGTCCAAGCTGGCGTTGTCGGGC
CAGCTGTTAAGCGGCGCGAACCGCTGCATGCCGCCCCCGGCGCCGCCGCGGCCGTCGTGGATGCGGTAGG
TGCCGGCAGCGTGCCACGCCATCCGGATAAACAGCGGCCCGTAGTGGCCGTAGTCGGCGGGCCACCACGG
CTGCGAGGTGGTCATCACTTCCTCGATGTCCCGCGTCAGGGCGTCAACGTCGATGGTCGCGACCTCCGCG
GCATAGTCGAACGCCGCACCCATCGGGTCAGCGACGGCCGGGTTTTGGTGCAGTACCTTCAGATTGAGCC
GGTTGGGCCACCAGTCCTGGTTTCCGCCGCCCTCGACGGGGTATTTCATATGACCCACGACGGGACAGCC
GTTGCTAGCGGCTCCGGTGGTGGTTTCTGTAATGGGTGGGTGTTGCTCGGGCA

Protein Sequence : Show Sequence

>gi|15609045|ref|NP_216424.1| catalase-peroxidase-peroxynitritase T KATG [Mycobacterium tuberculosis H37Rv]
MPEQHPPITETTTGAASNGCPVVGHMKYPVEGGGNQDWWPNRLNLKVLHQNPAVADPMGAAFDYAAEVAT
IDVDALTRDIEEVMTTSQPWWPADYGHYGPLFIRMAWHAAGTYRIHDGRGGAGGGMQRFAPLNSWPDNAS
LDKARRLLWPVKKKYGKKLSWADLIVFAGNCALESMGFKTFGFGFGRVDQWEPDEVYWGKEATWLGDERY
SGKRDLENPLAAVQMGLIYVNPEGPNGNPDPMAAAVDIRETFRRMAMNDVETAALIVGGHTFGKTHGAGP
ADLVGPEPEAAPLEQMGLGWKSSYGTGTGKDAITSGIEVVWTNTPTKWDNSFLEILYGYEWELTKSPAGA
WQYTAKDGAGAGTIPDPFGGPGRSPTMLATDLSLRVDPIYERITRRWLEHPEELADEFAKAWYKLIHRDM
GPVARYLGPLVPKQTLLWQDPVPAVSHDLVGEAEIASLKSQIRASGLTVSQLVSTAWAAASSFRGSDKRG
GANGGRIRLQPQVGWEVNDPDGDLRKVIRTLEEIQESFNSAAPGNIKVSFADLVVLGGCAAIEKAAKAAG
HNITVPFTPGRTDASQEQTDVESFAVLEPKADGFRNYLGKGNPLPAEYMLLDKANLLTLSAPEMTVLVGG
LRVLGANYKRLPLGVFTEASESLTNDFFVNLLDMGITWEPSPADDGTYQGKDGSGKVKWTGSRVDLVFGS
NSELRALVEVYGADDAQPKFVQDFVAAWDKVMNLDRFDVR

Molecule Role : Protective antigen
Molecule Role Annotation : DNA vaccines encoding KatG antigen fused to TPA signal sequences evoked a significant protective response in mice aerogenically challenged with low doses of Mycobacterium tuberculosis Erdman 17 to 21 days after the final immunization (Li et al., 1999).

11. Mpt63

Gene Name : Mpt63
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0011176
NCBI Gene ID : 885334
NCBI Protein GI : 15609063
Locus Tag : Rv1926c
Genbank Accession : U27119
Protein Accession : NP_216442
Taxonomy ID : 83332
Gene Starting Position : 2178956
Gene Ending Position : 2179435
Gene Strand (Orientation) : -
Protein Name : immunogenic protein MPT63 (antigen MPT63/MPB63) (16 kDa immunoprotective extracellular protein)
Protein pI : 4.67
Protein Weight : 14827.76
Protein Length : 159
Protein Note : Rv1926c, (MT1977, MTCY09F9.38), len: 159 aa. mpt63 (alternate gene name: mpb63), immunogenic protein (see citations below), identical to MPT63|MPB63 from Mycobacterium bovis (159 aa). Exported protein containing a N-terminal signal sequence: see notes below about proteomics.; mpb63

DNA Sequence : Show Sequence

>gi|57116681:2178956-2179435 Mycobacterium tuberculosis H37Rv, complete genome
TCTACGGCTCCCAAATCAGCAGATCCTCCATGCCGTTGTTCATCGCGACGATGGTTGGCGATGGGCCGGT
GACATCGAAGTAGATTTTGCCGGTCGATTGTTCGCCTTGGGGGATAGTGGCTCCGCTAATGGTGTCGGGG
CCCGCGGCTTGCCACAGCACCCGGTAGTTGATGCCGTCGGCGGTGCGGGCATTGAACTGCGAGACCGCGG
GCGTGACGCTGCCGCGAATCGCATTGACCGTGGCAGTGGCCTCCCAGACCTGGCCGGCCACCGGATAGCC
GGGGATGACTGCCGTGCTGGATTTGAGATCACTGACCTTCCAGCCGAGCACGACTTGGCCAACGGTGTCG
GTCATCGTTAGCTCACTGCCAAGTTTTCCGGTGATGGGATAGGCAGCCAACGCGACCGGTGCCGCAAAGG
TCGCGATGGCCGCCATGGCCACGACCGCTACTGCCGTCTTGATCATTGTGGTGAGCTTCA

Protein Sequence : Show Sequence

>gi|15609063|ref|NP_216442.1| immunogenic protein MPT63 (antigen MPT63/MPB63) (16 kDa immunoprotective extracellular protein) [Mycobacterium tuberculosis H37Rv]
MKLTTMIKTAVAVVAMAAIATFAAPVALAAYPITGKLGSELTMTDTVGQVVLGWKVSDLKSSTAVIPGYP
VAGQVWEATATVNAIRGSVTPAVSQFNARTADGINYRVLWQAAGPDTISGATIPQGEQSTGKIYFDVTGP
SPTIVAMNNGMEDLLIWEP

Molecule Role : Protective antigen
Molecule Role Annotation : MPT63 (Rv1926c), a major secreted protein of Mycobacterium tuberculosis, is immunoreactive in antibody assays in humans and animals and provides protection as a combined DNA vaccine in mice (Mustafa, 2009).

12. MPT64

Gene Name : MPT64
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 885925
NCBI Protein GI : 15609117
Locus Tag : Rv1980c
Genbank Accession : A42312
Protein Accession : NP_216496
Taxonomy ID : 83332
Gene Starting Position : 2223342
Gene Ending Position : 2224028
Gene Strand (Orientation) : -
Protein Name : immunogenic protein MPT64 (antigen MPT64/MPB64)
Protein pI : 4.6
Protein Weight : 23267.4
Protein Length : 228
Protein Note : Rv1980c, (MT2032, MTCY39.39), len: 228 aa. mpt64 (alternate gene name: mpb64), immunogenic protein (alternate gene name: mpb64) (see citations below), identical to MPT64|MPB64 from Mycobacterium bovis (228 aa). Similar to Rv3036c|MTV012.51c from Mycobacterium tuberculosis. Exported protein containing a N-terminal signal sequence: see notes below about proteomics.; mpb64

DNA Sequence : Show Sequence

>gi|57116681:2223342-2224028 Mycobacterium tuberculosis H37Rv, complete genome
TCTAGGCCAGCATCGAGTCGATCGCGGAACGTGGGACCAATACCTGGGTTGGGCCGGCTGCTTCGGGCAG
CAACTCCCCCGGGTTGAAGAAGAAAATCACCCCGTCGTTCGTGACTGCGAAGTTCTGATAATTCACCGGG
TCCAAGCCGGCATTCGGCGCTATCGATACCTGTTGTCCGGTCTGCTTGCTCAGTTCACCTTGCACAATGG
GGAAGACGACTGGCAGCGGATCGGTGTCAGCCTGCCACAGCGTGTCATAGGTGATTGGCTTGCGATAGGC
CTGGTCCCAATCGAAGGCCTTGTACGTGGTCGTTGGGTGCGTGCCGCCGGCGTTCTGGTAGACCTTGAGC
ACCACGGCCTGCGTACCACGCGGCGGTATCGCGGACTGGTATGTGGCCGAGGTGATATTCAATTCGTAGG
GGGCTTCGCGTGGAGTGGACGATGTGGCCGCGCTGAGGAACTTGTCGCGCGTCTGGGCGATGTAATTTTC
CAGCGACTTCTGGTCGGGGTAGTAACTGGGCAGGCTGATGTTGATGTTGTAGGCCGGGTCGGACATTTGA
ATCTGGCACGCCTGGCCGGTATCGGTGCCTTTCAACTCCTCGCAGTAGGTCTTGGGCGCGGCCGTGGCCA
CACCCGAACAACAGAGCAAAACGACAGCCGTGACCAGCATGAAGATCTTGATGCGCA

Protein Sequence : Show Sequence

>gi|15609117|ref|NP_216496.1| immunogenic protein MPT64 (antigen MPT64/MPB64) [Mycobacterium tuberculosis H37Rv]
MRIKIFMLVTAVVLLCCSGVATAAPKTYCEELKGTDTGQACQIQMSDPAYNINISLPSYYPDQKSLENYI
AQTRDKFLSAATSSTPREAPYELNITSATYQSAIPPRGTQAVVLKVYQNAGGTHPTTTYKAFDWDQAYRK
PITYDTLWQADTDPLPVVFPIVQGELSKQTGQQVSIAPNAGLDPVNYQNFAVTNDGVIFFFNPGELLPEA
AGPTQVLVPRSAIDSMLA

Molecule Role : Protective antigen
Molecule Role Annotation : DNA vaccines encoding secreted proteins Ag85B and MPT64 protect mice from the tuberculosis H37Rv strain (Luo et al., 2004).
*Note* MPT64 in combination with Ag85B was protective, DNA vaccine of just MPT64 was mildly protective in mice.

13. Mtb72F

Gene Name : Mtb72F
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis
VO ID : VO_0011173
NCBI Protein GI : 283801990
Taxonomy ID : 32630
Gene Strand (Orientation) : ?
Protein Length : 729
Protein Note : Description of Artificial Sequence:tri-fusion protein MTB72F (Ra12-TbH9-Ra35 or MTB32-MTB39 fusion)

Protein Sequence : Show Sequence

>gi|283801990|emb|CBI85484.1| unnamed protein product [synthetic construct]
MHHHHHHTAASDNFQLSQGGQGFAIPIGQAMAIAGQIRSGGGSPTVHIGPTAFLGLGVVDNNGNGARVQR
VVGSAPAASLGISTGDVITAVDGAPINSATAMADALNGHHPGDVISVTWQTKSGGTRTGNVTLAEGPPAE
FMVDFGALPPEINSARMYAGPGSASLVAAAQMWDSVASDLFSAASAFQSVVWGLTVGSWIGSSAGLMVAA
ASPYVAWMSVTAGQAELTAAQVRVAAAAYETAYGLTVPPPVIAENRAELMILIATNLLGQNTPAIAVNEA
EYGEMWAQDAAAMFGYAAATATATATLLPFEEAPEMTSAGGLLEQAAAVEEASDTAAANQLMNNVPQALQ
QLAQPTQGTTPSSKLGGLWKTVSPHRSPISNMVSMANNHMSMTNSGVSMTNTLSSMLKGFAPAAARQAVQ
TAAQNGVRAMSSLGSSLGSSGLGGGVAANLGRAASVGSLSVPQAWAAANQAVTPAARALPLTSLTSAAER
GPGQMLGGLPVGQMGARAGGGLSGVLRVPPRPYVMPHSPAAGDIAPPALSQDRFADFPALPLDPSAMVAQ
VGPQVVNINTKLGYNNAVGAGTGIVIDPNGVVLTNNHVIAGATDINAFSVGSGQTYGVDVVGYDRTQDVA
VLQLRGAGGLPSAAIGGGVAVGEPVVAMGNSGGQGGTPRAVPGRVVALGQTVQASDSLTGAEETLNGLIQ
FDAAIQPGDSGGPVVNGLGQVVGMNTAAS

Molecule Role : Protective antigen
Molecule Role Annotation : Our lead construct, Mtb72F, codes for a 72-kDa polyprotein genetically linked in tandem in the linear order Mtb32(C)-Mtb39-Mtb32(N). All forms of Mtb72F immunization tested resulted in the protection of C57BL/6 mice against aerosol challenge with a virulent strain of M. tuberculosis (Skeiky et al., 2004).
Related Vaccine(s): M. tuberculosis Mtb72F Protein Subunit Vaccine

14. PE20

Gene Name : PE20
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 885537
NCBI Protein GI : 57116919
Locus Tag : Rv1806
Genbank Accession : BX842577
Protein Accession : YP_177843
Taxonomy ID : 83332
Gene Starting Position : 2048071
Gene Ending Position : 2048370
Gene Strand (Orientation) : ?
Protein Name : PE family protein
Protein pI : 4.19
Protein Weight : 8927.3
Protein Length : 99
Protein Note : Rv1806, (MTV049.28), len: 99 aa. Member of the Mycobacterium tuberculosis PE family of gly-, ala-rich proteins (see citation below), most similar to Rv1788|MTV049.10|AL022021 (99 aa), FASTA scores: opt: 334, E(): 4.7 e-15, (59.8% identity in 97 aa overlap). TBparse score is 0.932.

DNA Sequence : Show Sequence

>gi|57116681:2048071-2048370 Mycobacterium tuberculosis H37Rv, complete genome
CATGGCGTTTGTTCTTGTCTGTCCAGATGCGCTGGCCATCGCGGCCGGTCAGTTGCGCCATGTTGGATCG
GTGATAGCCGCGCGGAATGCGGTCGCGGCACCGGCAACTGCCGAATTGGCCCCGGCGGCCGCTGACGAAG
TATCAGCTTTGACTGCAACACAATTCAACTTCCATGCCGCCATGTACCAAGCGGTCGGCGCCCAGGCGAT
CGCCATGAATGAGGCGTTCGTCGCGATGTTGGGCGCCAGCGCGGATTCTTACGCGGCTACCGAAGCCGCC
AACATCATTGCTGTGAGCTA

Protein Sequence : Show Sequence

>gi|57116919|ref|YP_177843.1| PE family protein [Mycobacterium tuberculosis H37Rv]
MAFVLVCPDALAIAAGQLRHVGSVIAARNAVAAPATAELAPAAADEVSALTATQFNFHAAMYQAVGAQAI
AMNEAFVAMLGASADSYAATEAANIIAVS

Molecule Role : Protective antigen
Molecule Role Annotation : A selection of previously identified protective Mycobacterium tuberculosis DNA vaccines were re-formulated as proteins and administered with a Th1-inducing adjuvant to help stimulate the relevant immune responses necessary for protection. One candidate (Rv1806-1807) induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M. tuberculosis in the lungs (Vipond et al., 2006).

15. PepA

Gene Name : PepA
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010953
NCBI Gene ID : 886924
NCBI Protein GI : 15607267
Locus Tag : Rv0125
Genbank Accession : NC_000962.2
Protein Accession : NC_000962.2
Taxonomy ID : 1773
Gene Starting Position : 151148
Gene Ending Position : 152215
Gene Strand (Orientation) : +
Protein Name : PROBABLE SERINE PROTEASE PEPA (SERINE PROTEINASE) (MTB32A)
Protein pI : 4.91
Protein Weight : 34926
Protein Length : 355
Protein Note : Rv0125, (MTCI418B.07, MTB32A), len: 355 aa. Probable pepA (alternate gene name: mtb32a), serine protease (EC 3.4.21.-) (see Skeiky et al., 1999), highly similar to other proteases e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa), FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in 346 aa overlap). Also similar to Q50320 34 kDa PROTEIN PRECURSOR from Mycobacterium tuberculosis (361 aa), FASTA scores: opt: 1689, E(): 0, (70.7% identity in 362 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Has a putative signal sequence at the N-terminus. BELONGS TO THE SERINE PROTEASE FAMILY.; mtb32a

DNA Sequence : Show Sequence

>GeneID|886924 [Mycobacterium tuberculosis H37Rv ] 151148..152215
atgagcaattcgcgccgccgctcactcaggtggtcatggttgctgagcgtgctggctgccgtcgggctgg
gcctggccacggcgccggcccaggcggccccgccggccttgtcgcaggaccggttcgccgacttccccgc
gctgcccctcgacccgtccgcgatggtcgcccaagtggggccacaggtggtcaacatcaacaccaaactg
ggctacaacaacgccgtgggcgccgggaccggcatcgtcatcgatcccaacggtgtcgtgctgaccaaca
accacgtgatcgcgggcgccaccgacatcaatgcgttcagcgtcggctccggccaaacctacggcgtcga
tgtggtcgggtatgaccgcacccaggatgtcgcggtgctgcagctgcgcggtgccggtggcctgccgtcg
gcggcgatcggtggcggcgtcgcggttggtgagcccgtcgtcgcgatgggcaacagcggtgggcagggcg
gaacgccccgtgcggtgcctggcagggtggtcgcgctcggccaaaccgtgcaggcgtcggattcgctgac
cggtgccgaagagacattgaacgggttgatccagttcgatgccgcgatccagcccggtgattcgggcggg
cccgtcgtcaacggcctaggacaggtggtcggtatgaacacggccgcgtccgataacttccagctgtccc
agggtgggcagggattcgccattccgatcgggcaggcgatggcgatcgcgggccagatccgatcgggtgg
ggggtcacccaccgttcatatcgggcctaccgccttcctcggcttgggtgttgtcgacaacaacggcaac
ggcgcacgagtccaacgcgtggtcgggagcgctccggcggcaagtctcggcatctccaccggcgacgtga
tcaccgcggtcgacggcgctccgatcaactcggccaccgcgatggcggacgcgcttaacgggcatcatcc
cggtgacgtcatctcggtgacctggcaaaccaagtcgggcggcacgcgtacagggaacgtgacattggcc
gagggacccccggcctga

Protein Sequence : Show Sequence

>gi|15607267|ref|NP_214639.1| PROBABLE SERINE PROTEASE PEPA (SERINE PROTEINASE) (MTB32A) [Mycobacterium tuberculosis H37Rv ]
MSNSRRRSLRWSWLLSVLAAVGLGLATAPAQAAPPALSQDRFADFPALPLDPSAMVAQVGPQVVNINTKL
GYNNAVGAGTGIVIDPNGVVLTNNHVIAGATDINAFSVGSGQTYGVDVVGYDRTQDVAVLQLRGAGGLPS
AAIGGGVAVGEPVVAMGNSGGQGGTPRAVPGRVVALGQTVQASDSLTGAEETLNGLIQFDAAIQPGDSGG
PVVNGLGQVVGMNTAASDNFQLSQGGQGFAIPIGQAMAIAGQIRSGGGSPTVHIGPTAFLGLGVVDNNGN
GARVQRVVGSAPAASLGISTGDVITAVDGAPINSATAMADALNGHHPGDVISVTWQTKSGGTRTGNVTLA
EGPPA

Molecule Role : Protective antigen
Related Vaccine(s): Mtb72f fusion protein

16. PPE14

Gene Name : PPE14
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 885069
NCBI Protein GI : 57116795
Locus Tag : Rv0915c
Genbank Accession : BX842574
Protein Accession : YP_177765
Taxonomy ID : 83332
Gene Starting Position : 1020057
Gene Ending Position : 1021328
Gene Strand (Orientation) : -
Protein Name : PPE family protein
Protein pI : 4.31
Protein Weight : 39057.75
Protein Length : 423
Protein Note : Rv0915c, (MTCY21C12.09c), len: 423 aa. PPE14 (alternate gene name: MTB41). Member of the Mycobacterium tuberculosis PPE family (see citation below), highly similar to many e.g. Rv1807 from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 966, E(): 4.4e-30, (45.7% identity in 392 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2.; MTB41

DNA Sequence : Show Sequence

>gi|57116681:1020057-1021328 Mycobacterium tuberculosis H37Rv, complete genome
TTTACCGCGGGGGGTTTCCGGGCGGCGGCTGCTCTCTAATCACAACTACCGGGGGTTTGCGGCCGTCCTC
TTGGCCGTCAGTGCTGGTGCCGCTACGGGTGCCGCCACCGCCCGTCGTGCCGCGTGCGGCCAGGCTCGCC
AAAGCCATCCCGCTGAGCAGGCCTGCCGGCATCCCGTTTAGGGCCGTCGGGTCGGCGCCGGCGCTGGAGC
TGAAGGTGGGTGTTGCCTGAACGGCGAGCTGGATCTCCGGGGCGGCCGTGGTCCAGCTGTGCGGCACCGA
CAACGCTCCGACTAATGCTGCGTGGCCGACGCCCGCGGACACCGGCGCCGCGCCCCCGAAGGGGCCCCAG
TGCGGCTCCGGCTCGTCGGTCGCCGAACTCAGTGGATGGCCCTGCGTCGGTCCCAGCCCGCCGGCGTTCC
CGTATAGGCCGATGTGCCAGGGTCTGGCCGTGTTCGTGATCGCGAGCGCAATGCTGCCGGTCGCGATGGA
TGCAATGTAGAGCGCGATCACGTCCAATTCCCCTATCGGGGTGGGGATCACTATCGGCTGAGCGGATCCG
ACTTGCGGGTTGAGGGTCGACGCGATCCCCAACAGTCCCGATGTCAGCGGATCAGCGTTGGCGGCCAATG
CGGACAGAATGTCGCTCAGGATCCCCGGGGGCAGCTGGGCCAGTGTCGCCTGTGCATCCGCAACGGCGCC
CGCACCGGCGGCTTGGGTCGCCGCGGCTGCGGCCGCGGGCCCGGCCGGGCCGGTGCCTTGCACGGGTGGA
GTGAACGGCGGCAACGCCGACGCGGCCGCAGATGCCCCCTCATAGCTGTACATCACGGCAGCGTCTTGGG
CCCACATTTCGGCATACTCGGCCTGGGTAGCCGCGATCGCCGCACTGTTTTGCCCCAGAATGTTCGCCGC
GACCAGCGACATCAACCGGCTGCGGTTGGCCGCGACGAGGGATGGTGGCACCGTCATCGCGAACGCCGTC
CCAAACGCTTCCGCCGCTGCCCTCGCCTGTGTGGCCGTCTCCTTCGCCAGCGCCGCCGTGGCGGCCAGCC
ACCCCACATACGGCGTTGCCGCGGCCGCCATCGCGGCCGCCGCCGGCCCCATCCACGGCTCAACGATCAG
CGTCGACACCACCGATCCATACGAGACCGCGGCGGAAGTCAACTCCGCGGCCACACCGTCCCAGGCGGCC
GCGGCGGCTAGCATCGACTCCGGCCCCGGACCGGAATACATTCGGCTTGAATTCACTTCCGGAGGTAAAA
GCCCGAAATCCA

Protein Sequence : Show Sequence

>gi|57116795|ref|YP_177765.1| PPE family protein [Mycobacterium tuberculosis H37Rv]
MDFGLLPPEVNSSRMYSGPGPESMLAAAAAWDGVAAELTSAAVSYGSVVSTLIVEPWMGPAAAAMAAAAT
PYVGWLAATAALAKETATQARAAAEAFGTAFAMTVPPSLVAANRSRLMSLVAANILGQNSAAIAATQAEY
AEMWAQDAAVMYSYEGASAAASALPPFTPPVQGTGPAGPAAAAAATQAAGAGAVADAQATLAQLPPGILS
DILSALAANADPLTSGLLGIASTLNPQVGSAQPIVIPTPIGELDVIALYIASIATGSIALAITNTARPWH
IGLYGNAGGLGPTQGHPLSSATDEPEPHWGPFGGAAPVSAGVGHAALVGALSVPHSWTTAAPEIQLAVQA
TPTFSSSAGADPTALNGMPAGLLSGMALASLAARGTTGGGGTRSGTSTDGQEDGRKPPVVVIREQPPPGN
PPR

Molecule Role : Protective antigen
Molecule Role Annotation : immunization with the soluble recombinant rMTB41 protein plus adjuvant, a regimen known to generate primarily CD4+ T cells, resulted in induction of protection comparable to BCG in two well-established animal models of tuberculosis (mice and guinea pigs) (Skeiky et al., 2005).

17. PPE18

Gene Name : PPE18
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010954
NCBI Gene ID : 886073
NCBI Protein GI : 57116841
Locus Tag : Rv1196
Genbank Accession : NC_000962.2
Protein Accession : NC_000962.2
Taxonomy ID : 1773
Gene Starting Position : 1339349
Gene Ending Position : 1340524
Gene Strand (Orientation) : +
Protein Name : PPE FAMILY PROTEIN
Protein pI : 4.81
Protein Weight : 39158.1
Protein Length : 391
Protein Note : Rv1196, (MTCI364.08), len: 391 aa. PPE18 (alternate gene name: mtb39a). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to others e.g. Y07P_MYCTU|Q11031 hypothetical 40.0 kDa protein cy02b10.25c (396 aa), FASTA scores: opt: 2124, E(): 0, (85.1% identity in 397 aa overlap). Note that expression of Rv1196 was demonstrated in lysates by immunodetection (see Dillon et al., 1999).; mtb39a

DNA Sequence : Show Sequence

>GeneID|886073 [Mycobacterium tuberculosis H37Rv ] 1339349..1340524
atggtggatttcggggcgttaccaccggagatcaactccgcgaggatgtacgccggcccgggttcggcct
cgctggtggccgcggctcagatgtgggacagcgtggcgagtgacctgttttcggccgcgtcggcgtttca
gtcggtggtctggggtctgacggtggggtcgtggataggttcgtcggcgggtctgatggtggcggcggcc
tcgccgtatgtggcgtggatgagcgtcaccgcggggcaggccgagctgaccgccgcccaggtccgggttg
ctgcggcggcctacgagacggcgtatgggctgacggtgcccccgccggtgatcgccgagaaccgtgctga
actgatgattctgatagcgaccaacctcttggggcaaaacaccccggcgatcgcggtcaacgaggccgaa
tacggcgagatgtgggcccaagacgccgccgcgatgtttggctacgccgcggcgacggcgacggcgacgg
cgacgttgctgccgttcgaggaggcgccggagatgaccagcgcgggtgggctcctcgagcaggccgccgc
ggtcgaggaggcctccgacaccgccgcggcgaaccagttgatgaacaatgtgccccaggcgctgcaacag
ctggcccagcccacgcagggcaccacgccttcttccaagctgggtggcctgtggaagacggtctcgccgc
atcggtcgccgatcagcaacatggtgtcgatggccaacaaccacatgtcgatgaccaactcgggtgtgtc
gatgaccaacaccttgagctcgatgttgaagggctttgctccggcggcggccgcccaggccgtgcaaacc
gcggcgcaaaacggggtccgggcgatgagctcgctgggcagctcgctgggttcttcgggtctgggcggtg
gggtggccgccaacttgggtcgggcggcctcggtcggttcgttgtcggtgccgcaggcctgggccgcggc
caaccaggcagtcaccccggcggcgcgggcgctgccgctgaccagcctgaccagcgccgcggaaagaggg
cccgggcagatgctgggcgggctgccggtggggcagatgggcgccagggccggtggtgggctcagtggtg
tgctgcgtgttccgccgcgaccctatgtgatgccgcattctccggcggccggctag

Protein Sequence : Show Sequence

>gi|57116841|ref|YP_177795.1| PPE FAMILY PROTEIN [Mycobacterium tuberculosis H37Rv ]
MVDFGALPPEINSARMYAGPGSASLVAAAQMWDSVASDLFSAASAFQSVVWGLTVGSWIGSSAGLMVAAA
SPYVAWMSVTAGQAELTAAQVRVAAAAYETAYGLTVPPPVIAENRAELMILIATNLLGQNTPAIAVNEAE
YGEMWAQDAAAMFGYAAATATATATLLPFEEAPEMTSAGGLLEQAAAVEEASDTAAANQLMNNVPQALQQ
LAQPTQGTTPSSKLGGLWKTVSPHRSPISNMVSMANNHMSMTNSGVSMTNTLSSMLKGFAPAAAAQAVQT
AAQNGVRAMSSLGSSLGSSGLGGGVAANLGRAASVGSLSVPQAWAAANQAVTPAARALPLTSLTSAAERG
PGQMLGGLPVGQMGARAGGGLSGVLRVPPRPYVMPHSPAAG

Molecule Role : Protective antigen
Related Vaccine(s): Mtb72f fusion protein

18. PPE31

Gene Name : PPE31
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 885072
NCBI Protein GI : 57116920
Locus Tag : Rv1807
Genbank Accession : BX842577
Protein Accession : YP_177653
Taxonomy ID : 83332
Gene Starting Position : 2048397
Gene Ending Position : 2049596
Gene Strand (Orientation) : ?
Protein Name : PPE family protein
Protein pI : 4.25
Protein Weight : 36883.49
Protein Length : 399
Protein Note : Rv1807, (MTV049.29), len: 399 aa. Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv1789|MTV049.11|AL022021 (393 aa), FASTA scores: opt: 1169, E(): 0, (49.5% identity in 412 aa overlap). TBparse score is 0.919.

DNA Sequence : Show Sequence

>gi|57116681:2048397-2049596 Mycobacterium tuberculosis H37Rv, complete genome
ACTTGACTTCGCCACGCTACCGCCCGAAATCAACTCGGCGCGTATGTATTCCGGCGCGGGCTCGGCCCCG
ATGCTGGCCGCAGCGTCAGCCTGGCACGGCTTGTCCGCAGAACTGCGCGCCAGCGCACTGTCATACAGCT
CGGTGCTTTCGACGCTGACCGGTGAAGAATGGCACGGTCCGGCGTCGGCATCGATGACAGCCGCGGCCGC
CCCCTACGTGGCCTGGATGAGCGTCACCGCCGTCCGGGCCGAGCAGGCCGGGGCACAGGCGGAGGCTGCC
GCTGCAGCGTACGAAGCCGCGTTCGCAGCAACGGTGCCCCCGCCGGTCATCGAGGCCAACCGCGCCCAGC
TCATGGCGCTGATCGCCACCAATGTGCTAGGCCAAAACGCCCCCGCGATCGCGGCCACCGAGGCCCAGTA
CGCCGAAATGTGGTCCCAGGACGCGATGGCCATGTACGGCTACGCCGGCGCCTCGGCAGCCGCTACCCAG
CTGACCCCGTTCACCGAGCCGGTGCAGACTACCAACGCGTCCGGCCTGGCGGCCCAGTCGGCTGCGATTG
CCCACGCCACCGGCGCCTCGGCTGGTGCTCAGCAAACGACGCTGTCGCAGCTGATCGCCGCCATACCGTC
TGTACTGCAAGGACTTTCGTCATCGACTGCAGCCACGTTCGCGTCGGGGCCGTCCGGATTGCTGGGCATT
GTCGGGTCTGGATCTTCCTGGCTCGACAAACTCTGGGCGTTACTGGACCCCAACTCCAATTTCTGGAACA
CGATAGCTTCGTCCGGACTGTTCTTGCCGAGTAACACGATTGCGCCCTTTTTGGGTCTACTCGGCGGCGT
GGCAGCTGCGGATGCGGCCGGGGATGTGTTGGGAGAGGCCACCAGTGGCGGGCTCGGTGGCGCGCTGGTG
GCGCCGCTTGGCTCAGCGGGCGGGCTAGGCGGCACTGTCGCGGCCGGCCTGGGCAACGCGGCCACCGTCG
GAACCTTGTCGGTGCCGCCGAGCTGGACGGCGGCCGCACCACTAGCCAGCCCCTTGGGCTCCGCGTTGGG
AGGCACACCGATGGTGGCACCGCCCCCAGCAGTGGCGGCCGGCATGCCCGGAATGCCTTTCGGCACCATG
GGCGGTCAAGGCTTCGGGCGTGCCGTGCCCCAGTATGGCTTCCGCCCCAACTTCGTCGCACGACCGCCCG
CCGCCGGGTG

Protein Sequence : Show Sequence

>gi|57116920|ref|YP_177653.1| PPE family protein [Mycobacterium tuberculosis H37Rv]
LDFATLPPEINSARMYSGAGSAPMLAAASAWHGLSAELRASALSYSSVLSTLTGEEWHGPASASMTAAAA
PYVAWMSVTAVRAEQAGAQAEAAAAAYEAAFAATVPPPVIEANRAQLMALIATNVLGQNAPAIAATEAQY
AEMWSQDAMAMYGYAGASAAATQLTPFTEPVQTTNASGLAAQSAAIAHATGASAGAQQTTLSQLIAAIPS
VLQGLSSSTAATFASGPSGLLGIVGSGSSWLDKLWALLDPNSNFWNTIASSGLFLPSNTIAPFLGLLGGV
AAADAAGDVLGEATSGGLGGALVAPLGSAGGLGGTVAAGLGNAATVGTLSVPPSWTAAAPLASPLGSALG
GTPMVAPPPAVAAGMPGMPFGTMGGQGFGRAVPQYGFRPNFVARPPAAG

Molecule Role : Protective antigen
Molecule Role Annotation : A selection of previously identified protective Mycobacterium tuberculosis DNA vaccines were re-formulated as proteins and administered with a Th1-inducing adjuvant to help stimulate the relevant immune responses necessary for protection. One candidate (Rv1806-1807) induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M. tuberculosis in the lungs (Vipond et al., 2006).

19. PstS3

Gene Name : PstS3
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 885366
NCBI Protein GI : 57116798
Locus Tag : Rv0928
Genbank Accession : BX842575
Protein Accession : YP_177768
Taxonomy ID : 83332
Gene Starting Position : 1034902
Gene Ending Position : 1036014
Gene Strand (Orientation) : ?
Protein Name : periplasmic phosphate-binding lipoprotein PSTS3 (PBP-3) (PSTS3) (PHOS1)
Protein pI : 5.85
Protein Weight : 35472.01
Protein Length : 370
Protein Note : Rv0928, (MTCY21C12.22), len: 370 aa. pstS3 (previously known as phoS2), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to others from Mycobacterium leprae e.g. Q50099|PSTS3|PHOS1 phosphate-binding protein 3 precursor (328 aa), FASTA scores: opt: 1772, E(): 0, (79.6% identity in 328 aa overlap); and highly similar to others e.g. AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa). Also highly similar to Rv0932c|MTCY08D9.07|pstS2 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN (370 aa); and Rv0934|pstS1 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN (374 aa) from Mycobacterium tuberculosis (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0934c). Contains lipoprotein signature (PS00013) at N-terminus. BELONGS TO FAMILY OF PHOSPHATE RECEPTORS FOR BACTERIAL ABC-TYPE LIPOPROTEIN TRANSPORTERS.; phoS2

DNA Sequence : Show Sequence

>gi|57116681:1034902-1036014 Mycobacterium tuberculosis H37Rv, complete genome
ATTGAAACTCAACCGATTTGGTGCCGCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGT
GGTAACGACGACAATGTGACCGGGGGAGGTGCAACCACTGGCCAGGCGTCGGCGAAGGTCGATTGCGGGG
GGAAGAAGACACTCAAAGCCAGTGGGTCGACGGCGCAGGCCAACGCGATGACCCGCTTTGTCAACGTGTT
CGAGCAGGCCTGCCCCGGCCAAACCCTGAACTACACGGCCAATGGTTCGGGCGCTGGAATCAGCGAATTT
AATGGCAACCAAACCGATTTCGGTGGCTCAGATGTACCCCTGAGCAAGGACGAGGCCGCAGCGGCGCAGC
GGCGTTGCGGCTCGCCGGCGTGGAATCTGCCGGTGGTGTTCGGCCCGATCGCGGTTACCTACAACCTCAA
CAGCGTTTCCTCGCTAAATTTGGACGGCCCCACGTTGGCGAAGATCTTCAACGGCTCCATTACGCAGTGG
AACAATCCCGCGATCCAGGCGCTGAACCGCGACTTCACGCTGCCAGGTGAGCGGATTCACGTGGTGTTCC
GCAGCGATGAGTCGGGGACCACGGACAACTTCCAGAGGTACCTGCAGGCCGCGTCCAACGGTGCGTGGGG
TAAGGGCGCTGGAAAGTCGTTCCAAGGCGGCGTCGGTGAGGGCGCGCGGGGTAACGATGGCACGTCAGCG
GCCGCGAAGAACACCCCGGGGTCGATCACCTACAACGAGTGGTCGTTCGCCCAGGCGCAGCACCTGACCA
TGGCCAACATCGTCACTTCGGCTGGTGGGGACCCGGTGGCGATTACTATCGACTCGGTCGGCCAGACGAT
CGCCGGGGCCACCATCTCCGGGGTGGGCAACGACCTGGTGCTCGACACGGACTCGTTCTACCGGCCGAAG
CGTCCCGGCTCCTATCCGATCGTGTTAGCGACATACGAAATCGTTTGCTCGAAGTATCCCGACTCGCAGG
TTGGCACGGCTGTGAAGGCGTTCCTGCAGAGCACTATCGGCGCCGGTCAAAGCGGCCTGGGGGACAACGG
ATACATCCCAATTCCGGACGAGTTCAAATCGAGGCTGTCGACTGCGGTCAACGCGATCGCCTG

Protein Sequence : Show Sequence

>gi|57116798|ref|YP_177768.1| periplasmic phosphate-binding lipoprotein PSTS3 (PBP-3) (PSTS3) (PHOS1) [Mycobacterium tuberculosis H37Rv]
MKLNRFGAAVGVLAAGALVLSACGNDDNVTGGGATTGQASAKVDCGGKKTLKASGSTAQANAMTRFVNVF
EQACPGQTLNYTANGSGAGISEFNGNQTDFGGSDVPLSKDEAAAAQRRCGSPAWNLPVVFGPIAVTYNLN
SVSSLNLDGPTLAKIFNGSITQWNNPAIQALNRDFTLPGERIHVVFRSDESGTTDNFQRYLQAASNGAWG
KGAGKSFQGGVGEGARGNDGTSAAAKNTPGSITYNEWSFAQAQHLTMANIVTSAGGDPVAITIDSVGQTI
AGATISGVGNDLVLDTDSFYRPKRPGSYPIVLATYEIVCSKYPDSQVGTAVKAFLQSTIGAGQSGLGDNG
YIPIPDEFKSRLSTAVNAIA

Molecule Role : Protective antigen
Molecule Role Annotation : Mice vaccinated with PstS-3 DNA demonstrated significant and sustained reduction in bacterial CFU numbers in spleen and lungs for 3 mo after M. tuberculosis challenge, as compared with CFU counts in mice vaccinated with control DNA (Tanghe et al., 1999).

20. SecA2

Gene Name : SecA2
Sequence Strain (Speices/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010950
NCBI Gene ID : 885594
NCBI Protein GI : 15608958
Locus Tag : Rv1821
Genbank Accession : BX842577
Protein Accession : NP_216337
Other Database IDs : EMBL: AE000516
EMBL: BX842577
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
HSSP: O05885
InterPro: IPR000185
InterPro: IPR001650
InterPro: IPR011115
InterPro: IPR011116
InterPro: IPR011130
InterPro: IPR014018
InterPro: IPR014021
KEGG: mtc:MT1869
KEGG: mtu:Rv1821
Pfam: PF01043
Pfam: PF07516
Pfam: PF07517
PIR: F70720
PRINTS: PR00906
PROSITE: PS01312
PROSITE: PS51196
TIGR: MT1869
TubercuList: Rv1821
UniProt: P66785
Taxonomy ID : 83332
Gene Starting Position : 2066456
Gene Ending Position : 2068882
Gene Strand (Orientation) : +
Protein Name : preprotein translocase subunit SecA
Protein pI : 5.11
Protein Weight : 82502.57
Protein Length : 808
Protein Note : SecA2; functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond to SecA2; which is non-essential and seems to play a role in secretion of a subset of proteins
Protein Annotation : FUNCTION: Involved in protein export. Interacts with the secY/secE subunits. SecA has a central role in coupling the hydrolysis of ATP to the transfer of pre-secretory proteins across the membrane (By similarity) (UniProt: P66785)

SUBUNIT: Part of the prokaryotic protein translocation apparatus which comprise secA, secB, secD, secE, secF, secG and secY (By similarity) (UniProt: P66785)

SUBCELLULAR LOCATION: Cell membrane; peripheral membrane protein; cytoplasmic side (By similarity) (UniProt: P66785)

SIMILARITY: Belongs to the secA family (UniProt: P66785)

DNA Sequence : Show Sequence

>gi|57116681:2066456-2068882 Mycobacterium tuberculosis H37Rv, complete genome
CGTGAACGTGCACGGTTGTCCACGAATTGCGGCCTGTCGGTGTACAGACACGCACCCTCGCGGCCGGCCG
GCATTCGCGTACCGTTGGTTTGTGCCCAAGACCACCCGCGCTCAACCCGGCCGGCTGAGCAGCCGATTCT
GGCGATTGCTCGGCGCCAGCACCGAAAAGAACCGGAGCCGCTCCCTGGCGGATGTAACCGCTTCGGCAGA
ATACGACAAGGAAGCTGCCGATCTGTCCGACGAGAAGCTGCGTAAGGCGGCAGGCCTGCTCAACCTCGAC
GACCTCGCGGAGTCCGCCGATATCCCGCAGTTTCTCGCGATTGCCCGGGAAGCCGCCGAGCGGAGGACCG
GGCTGCGACCATTTGATGTGCAGTTGCTTGGCGCGTTGCGCATGCTCGCCGGAGACGTGATCGAGATGGC
CACCGGTGAGGGCAAAACCCTTGCCGGGGCGATCGCGGCCGCCGGTTATGCGCTGGCCGGCCGGCACGTG
CACGTCGTGACGATTAACGATTACCTGGCCCGCCGCGATGCGGAGTGGATGGGCCCGCTGCTGGACGCGA
TGGGCCTGACGGTCGGCTGGATCACCGCGGACTCGACCCCTGACGAGCGCCGGACCGCATATGACCGTGA
TGTCACCTATGCCTCGGTCAACGAGATTGGCTTCGATGTACTGCGCGATCAGTTGGTGACTGATGTCAAT
GACCTGGTATCGCCCAATCCAGACGTGGCTCTCATCGACGAAGCCGACTCCGTGCTGGTCGACGAGGCGC
TGGTGCCCCTGGTGCTGGCCGGAACCACACATCGTGAGACGCCGCGGCTGGAGATCATCCGGCTGGTCGC
TGAGCTTGTTGGCGACAAGGACGCCGACGAGTACTTTGCCACCGATTCCGATAACCGCAATGTCCACTTG
ACCGAGCACGGGGCACGCAAAGTCGAGAAAGCGCTCGGTGGCATCGACCTGTACTCCGAGGAGCACGTCG
GCACCACACTGACTGAGGTCAATGTCGCGCTGCACGCGCATGTGCTCCTGCAACGCGACGTGCACTACAT
CGTCCGCGACGACGCGGTGCACCTGATCAACGCGTCGCGTGGCCGTATCGCGCAACTGCAGCGCTGGCCG
GACGGGTTGCAAGCTGCGGTCGAGGCCAAGGAAGGTATCGAGACCACGGAAACTGGGGAAGTGCTCGACA
CCATCACGGTGCAGGCCCTGATCAACCGGTATGCGACTGTGTGCGGAATGACGGGAACCGCGCTGGCCGC
CGGTGAGCAGCTACGGCAGTTCTACCAGCTCGGTGTCTCACCGATACCACCGAACAAGCCAAACATCCGC
GAGGACGAGGCCGACCGGGTCTACATCACCACTGCAGCCAAGAACGACGGGATCGTCGAGCACATCACCG
AGGTGCACCAGAGGGGGCAGCCTGTGCTGGTCGGTACCCGCGACGTGGCCGAATCCGAGGAACTGCACGA
ACGCCTGGTGCGCCGCGGTGTGCCCGCCGTGGTGCTCAACGCGAAGAACGACGCCGAGGAGGCCCGGGTC
ATCGCCGAGGCCGGCAAATACGGCGCGGTCACGGTGTCAACTCAAATGGCCGGGCGCGGCACCGACATCA
GGCTCGGCGGGTCCGACGAAGCTGACCACGACAGGGTCGCGGAATTGGGCGGCCTGCACGTGGTCGGCAC
TGGCCGTCACCACACCGAGCGGCTAGACAACCAGCTGCGCGGTCGGGCCGGGCGCCAGGGAGATCCCGGG
TCGTCGGTGTTTTTCTCAAGCTGGGAAGACGATGTCGTTGCGGCCAACCTCGACCACAACAAGCTGCCGA
TGGCAACCGACGAAAATGGCCGGATTGTCAGCCCGAGGACGGGTAGTCTGCTCGACCATGCCCAGCGCGT
TGCCGAGGGCCGGTTATTGGATGTGCACGCCAACACGTGGCGCTACAACCAGCTGATCGCCCAGCAGCGC
GCCATCATCGTCGAACGGCGTAACACGTTGTTGCGCACCGTAACCGCGCGTGAGGAACTCGCCGAACTGG
CGCCTAAGCGGTACGAGGAGCTGTCCGACAAAGTATCCGAGGAACGCCTCGAGACGATTTGTCGGCAGAT
CATGCTGTATCACCTCGACCGTGGCTGGGCCGATCACCTGGCGTATCTGGCCGACATCCGGGAGAGCATC
CATCTACGCGCGCTGGGCCGGCAGAACCCACTCGACGAGTTTCACCGGATGGCTGTGGACGCGTTCGCGT
CGCTGGCCGCCGACGCCATCGAGGCGGCTCAACAGACGTTCGAAACCGCGAACGTCCTTGACCACGAGCC
GGGGCTGGACCTGTCCAAACTGGCCCGGCCGACGTCGACATGGACCTACATGGTCAATGACAACCCACTG
TCCGATGACACGCTTTCTGCCCTCAGTCTGCCCGGGGTGTTCCGCTG

Protein Sequence : Show Sequence

>gi|15608958|ref|NP_216337.1| preprotein translocase subunit SecA [Mycobacterium tuberculosis H37Rv]
MNVHGCPRIAACRCTDTHPRGRPAFAYRWFVPKTTRAQPGRLSSRFWRLLGASTEKNRSRSLADVTASAE
YDKEAADLSDEKLRKAAGLLNLDDLAESADIPQFLAIAREAAERRTGLRPFDVQLLGALRMLAGDVIEMA
TGEGKTLAGAIAAAGYALAGRHVHVVTINDYLARRDAEWMGPLLDAMGLTVGWITADSTPDERRTAYDRD
VTYASVNEIGFDVLRDQLVTDVNDLVSPNPDVALIDEADSVLVDEALVPLVLAGTTHRETPRLEIIRLVA
ELVGDKDADEYFATDSDNRNVHLTEHGARKVEKALGGIDLYSEEHVGTTLTEVNVALHAHVLLQRDVHYI
VRDDAVHLINASRGRIAQLQRWPDGLQAAVEAKEGIETTETGEVLDTITVQALINRYATVCGMTGTALAA
GEQLRQFYQLGVSPIPPNKPNIREDEADRVYITTAAKNDGIVEHITEVHQRGQPVLVGTRDVAESEELHE
RLVRRGVPAVVLNAKNDAEEARVIAEAGKYGAVTVSTQMAGRGTDIRLGGSDEADHDRVAELGGLHVVGT
GRHHTERLDNQLRGRAGRQGDPGSSVFFSSWEDDVVAANLDHNKLPMATDENGRIVSPRTGSLLDHAQRV
AEGRLLDVHANTWRYNQLIAQQRAIIVERRNTLLRTVTAREELAELAPKRYEELSDKVSEERLETICRQI
MLYHLDRGWADHLAYLADIRESIHLRALGRQNPLDEFHRMAVDAFASLAADAIEAAQQTFETANVLDHEP
GLDLSKLARPTSTWTYMVNDNPLSDDTLSALSLPGVFR

Molecule Role : Protective antigen
Molecule Role Annotation : Vaccination of mice and guinea pigs with a secA2 mutant significantly increased resistance to M. tuberculosis challenge compared with standard M. bovis bacille Calmette-Guérin vaccination (Hinchey et al., 2007).
Related Vaccine(s): M. tuberculosis secA2 mutant

II. References

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