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rfaL
Gene Name	rfaL
Sequence Strain (Species/Organism)	Escherichia coli str. K-12 substr. MG1655
NCBI Gene ID	948148
NCBI Protein GI	16131493
Locus Tag	b3622
Genbank Accession	U00096
Protein Accession	NP_418079
Taxonomy ID	511145
Gene Starting Position	3794970
Gene Ending Position	3796229
Gene Strand (Orientation)	+
Protein Name	O-antigen ligase
Protein pI	9.92
Protein Weight	44891.56
Protein Length	419
Protein Note	Also known as ECK3612; JW3597; waaLO-antigen ligase; lipopolysaccharide core biosynthesis
DNA Sequence	>gi\|49175990:3794970-3796229 Escherichia coli str. K-12 substr. MG1655 chromosome, complete genome CATGCTAACATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATA ATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATAT ATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAA AAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAA GTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTT CTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTA TTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCT TTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTAATAGGGATAGTTAGTGGCGTTGCGA TTCTTTATACTAAGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGC GCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTAT AATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTA TATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAA TAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCT TTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAA GAGGGGCATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCT GATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAGCTTTG GGTTTGTTGATATTAACGCTTGGCATTGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCA TTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTA
Protein Sequence	>gi\|16131493\|ref\|NP_418079.1\| O-antigen ligase [Escherichia coli str. K-12 substr. MG1655] MLTSFKLHSLKPYTLKSSMILEIITYILCFFSMIIAFVDNTFSIKIYNITAIVCLLSLILRGRQENYNIK NLILPLSIFLIGLLDLIWYSAFKVDNSPFRATYHSYLNTAKIFIFGSFIVFLTLTSQLKSKKESVLYTLY SLSFLIAGYAMYINSIHENDRISFGVGTATGAAYSTMLIGIVSGVAILYTKKNHPFLFLLNSCAVLYVLA LTQTRATLLLFPIICVAALIAYYNKSPKKFTSSIVLLIAILASIVIIFNKPIQNRYNEALNDLNSYTNAN SVTSLGARLAMYEIGLNIFIKSPFSFRSAESRAESMNLLVAEHNRLRGALEFSNVHLHNEIIEAGSLKGL MGIFSTLFLYFSLFYIAYKKRALGLLILTLGIVGIGLSDVIIWARSIPIIIISAIVLLLVINNRNNTIN
Molecule Role	Virmugen
Molecule Role Annotation	Deletion of waaL results in a strain that stimulates enhanced urothelial cytokine secretion, which is an enhanced innate immune response. Inoculation with the vaccine strain protected mice against challenge with a broad range of clinical uropathogenic E. coli isolates and produced immunity that lasted ⩾8 weeks (Billips et al., 2009).
Related Vaccines(s)	Escherichia coli rfaL mutant vaccine
References

rfaL