VIOLIN: Vaccine Investigation and Online Information Network

Yersinia pseudotuberculosis

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. opJ (Protective antigen)
2. Porin (Protective antigen)
3. pYV0055 (Protective antigen)
4. yopE (Protective antigen)
5. phoP (Virmugen)
Vaccine Information
1. Yersinia pseudotuberculosis phoP mutant vaccine
References

I. General Information

1. NCBI Taxonomy ID:

633

2. Disease:

Yersiniosis

3. Introduction

Yersinia pseudotuberculosis is a Gram-negative bacterium which primarily causes disease in animals; humans occasionally get infected zoonotically, most often through the food-borne route. In animals, Y. pseudotuberculosis can cause tuberculosis-like symptoms, including localized tissue necrosis and granulomas in the spleen, liver, and lymph node. In humans, symptoms are similar to those of infection with Yersinia enterocolitica (fever and right-sided abdominal pain), except that the diarrheal component is often absent, which sometimes makes the resulting condition difficult to diagnose. Y. pseudotuberculosis infections can mimic appendicitis, especially in children and younger adults, and, in rare cases the disease may cause skin complaints (erythema nodosum), joint stiffness and pain (reactive arthritis), or spread of bacteria to the blood (bacteremia) (Wiki: Y. pseudotuberuclosis).

II. Vaccine Related Pathogen Genes

1. opJ

Gene Name : opJ
Sequence Strain (Species/Organism) : Yersinia pseudotuberculosis
NCBI Protein GI : AAT28340
Other Database IDs : CDD:185269
Taxonomy ID : 633
Protein Name : outer protein J
Protein pI : 7.27
Protein Weight : 31206.2
Protein Length : 362
Protein Note : YopJ

Protein Sequence : Show Sequence

>AAT28340.1 outer protein J (plasmid) [Yersinia pseudotuberculosis]
MIGPISQINISGGLSEKETSSLISNEELKNIITQLETDISDGSWFHKNYSRMDVEVMPALVIQANNKYPE
MNLNLVTSPLDLSIEIKNVIENGVRSSRFIINMGEGGIHFSVIDYKHINGKTSLILFEPANFNSMGPAML
AIRTKTAIERYQLPDCHFSMVEMDIQRSSSECGIFSFALAKKLYIERDSLLKIHEDNIKGILSDGENPLP
HDKLDPYLPVTFYKHTQGKKRLNEYLNTNPQGVGTVVNKKNETIVNRFDNNKSIVDGKELSVSVHKKRIA
EYKTLLKV

Molecule Role : Protective antigen
Molecule Role Annotation : (Zhang and Bliska, 2010)

2. phoP

Gene Name : phoP
Sequence Strain (Species/Organism) : Yersinia pseudotuberculosis IP 32953
NCBI Gene ID : 2956268
NCBI Protein GI : 51596756
Locus Tag : YPTB2434
Genbank Accession : BX936398
Protein Accession : YP_070947
Taxonomy ID : 273123
Gene Starting Position : 2877188
Gene Ending Position : 2877859
Gene Strand (Orientation) : +
Protein Name : DNA-binding transcriptional regulator PhoP
Protein pI : 5.6
Protein Weight : 23966.83
Protein Length : 223
Protein Note : response regulator in two-component regulatory system with PhoQ; involved in magnesium starvation and stress

DNA Sequence : Show Sequence

>gi|51594359:2877188-2877859 Yersinia pseudotuberculosis IP 32953, complete genome
TATGCGGGTTCTGGTTGTGGAAGATAACGCGTTGTTGCGTCACCATCTGACAGTGCAAATGCGTGAAATG
GGCCATCAGGTTGATGCCGCGGAAGATGCTAAAGAAGCAGACTATTTCTTACAAGAGCATGCCCCCGACA
TTGCTATTATCGATCTTGGTTTGCCCGGTGAAGACGGGTTAAGCCTTATCCGTCGCTGGCGTAGCCATCA
AGAAAACTTACCCATTTTGGTCCTGACCGCACGGGAAAGCTGGCAAGATAAAGTCGCTGTACTGGAAGCC
GGTGCCGACGATTATGTGACCAAACCGTTCCATCTGGAAGAGGTCATTGCCCGGATGCAAGCACTGATGC
GGCGGAATATTGGCCTGGCCTCCCAAATTATTGAGCTACCGCCCTTCCAGATTGACCTTTCACGCCGTGA
GTTATGTGTTAACCAACAACAAATTAAACTGACGGCATTTGAATACACTATCATTGAAACCCTTATCCGC
AACGCAGGGAAAGTCGTTAGTAAGGATACGTTGATGCTACAACTTTATCCGGATGCGGAATTGCGAGAGA
GCCATACTATCGATGTATTAATGGGTCGACTACGTAAAAAACTGCTGGCAGAACATCAGGGTGAAGTCAT
TACGACTATTCGTGGTCAGGGATATCGTTTTGACGTCAACTA

Protein Sequence : Show Sequence

>gi|51596756|ref|YP_070947.1| DNA-binding transcriptional regulator PhoP [Yersinia pseudotuberculosis IP 32953]
MRVLVVEDNALLRHHLTVQMREMGHQVDAAEDAKEADYFLQEHAPDIAIIDLGLPGEDGLSLIRRWRSHQ
ENLPILVLTARESWQDKVAVLEAGADDYVTKPFHLEEVIARMQALMRRNIGLASQIIELPPFQIDLSRRE
LCVNQQQIKLTAFEYTIIETLIRNAGKVVSKDTLMLQLYPDAELRESHTIDVLMGRLRKKLLAEHQGEVI
TTIRGQGYRFDVN

Molecule Role : Virmugen
Molecule Role Annotation : A phoP mutant in Yersinia pseudotuberculosis (Yptb) was attenuated and provided protection against oral challenge with virulent Yptb in mice (Balada-Llasat et al., 2007).
Related Vaccine(s): Yersinia pseudotuberculosis phoP mutant vaccine

3. Porin

Gene Name : Porin
Sequence Strain (Species/Organism) : Yersinia pseudotuberculosis
NCBI Protein GI : WP_050103071
Other Database IDs : CDD:304382
CDD:290340
CDD:238208
Taxonomy ID : 633
Protein Name : porin OmpF2
Protein pI : 5.49
Protein Weight : 39303.36
Protein Length : 436
Protein Note : Porin superfamily. These outer membrane channels share a beta-barrel structure that differ in strand and shear number. Classical (gram-negative) porins are non-specific channels for small hydrophillic molecules and form 16 beta-stranded barrels (16,20)...; cl21487

Protein Sequence : Show Sequence

>WP_050103071.1 porin OmpF2 [Yersinia pseudotuberculosis]
MKRNILAILIPTLLVATTSHAAEIYNKDGNKLDLYGKVKALHYFSDNTKSDGDKSYVRLGFKGVTQITDE
LSGYGQWEYNFAANYAESQEAKDNKTRLAFAGLRYSNLGSIDYGRNYGVLYDIAAWTDMLPEFGNDSYTR
TDNFMTGRTTGVATYRNTDFFGLVDGLKFSLQYQGKNGAEGETNNGRADTSKQNGDGFGLSSSYEIGAGV
SVGAAYASSNRTLAQKNSTFGKGDKADAWTTGLKYSDNGVYLAATYAETRNMTPISGTAVINNVSTSVSG
FANKTQNIELVAQYLFDFGLKPSIAYIQSKGKDIEGIGDTDLVKYVDIGATYYFNKNMSTYVDYKINQLN
DDNKLKLNTDNVVALGLVYQF

Molecule Role : Protective antigen
Molecule Role Annotation : (Tsybul'ski? et al., 2011)

4. pYV0055

Gene Name : pYV0055
Sequence Strain (Species/Organism) : Yersinia pseudotuberculosis IP 32953
VO ID : VO_0011016
NCBI Gene ID : 2952948
NCBI Protein GI : 51593897
Locus Tag : pYV0055
Genbank Accession : BX936399
Protein Accession : YP_068464
Taxonomy ID : 273123
Plasmid No : pYV
Gene Starting Position : 37489
Gene Ending Position : 38694
Gene Strand (Orientation) : -
Protein Name : yopB; Yop targeting protein
Protein pI : 7.3
Protein Weight : 38322.89
Protein Length : 401
Protein Note : similar to Y. pestis YPCD1.29c

DNA Sequence : Show Sequence

>NC_006153.2:37489-38694 Yersinia pseudotuberculosis IP32953 pYV plasmid, complete sequence
CTTAAACAGTATGGGGTCTGCCGGCCAAATTATGCAGCATGTCACCTTTTGCATTAATCATCTGGAAAAT
CAGTTCCATCACTTGTTGATGTGACTCTGACAAATGGGATAATTCTTCTTTGAGTCGGTCAATAATACTT
TGTAACGCTGCCATGTCTGCCTTGTTCAAGGCCAAATCAGCGTTATTATGTTGTAAATCAGCTTGCTTTT
TATTATTGATGGTCTGAGTTATTCCGCTGCCCACACCAACGGCGACTTGTGTTGCCTGTGATCCTGTACG
GATTGCATGGCTCATTGTTAAACCAGCAAAACTGCCCCCTAATTTAGTCACTGCACTCCCGACAGTGTTT
GATATGCCTGTCGAAATTTGGGCAACTTTGGCCGAAAACTCGGCTCCTTTAGCCGCAAGACTTGCGGTGT
TAGCACCGAGTTTTGCGCCAATATTAGCCAGGCATTTTAGTGCCGAACCGCCAAAGGTCATTACGGTTGA
AACTACAGTCAATGCGACTTCAATCGCAGTGAGTATCGGCCCTAATATTTTCATTGCCTCTTGGGATATC
AGGCCATCTTCCGCCGCTTGTTTCACTGCCATATTCGCCATCCCAATTACGCCTGAGGCAACCATCATTG
CACCGGCAACGGCTCCTACCCCTGAGGCCACCATGATGGCACCGACAATCACTGAGGCTATGGCGCTGAG
CCAGCCAAAAATCTTTGATGCAATGCCGGATTTCTTGACTTGCTTGGCATTCTCTTCTGTTTCTTTGATT
TTCTCTTGATTCTCTTCAATTTTCTTCATGTTTTGTTCATGAAGACGCTGAATATCAGCGATTTTTATGT
CTTTGCGTACTTCTTCTAAATTAGAAACTAGCTTACCTAACTCAATCTCAAATGTATCAGGTGAAGCAAG
CGTAAACTTCGTTAGCACTGAAACATAATTTGATATCAGTTGAGATCCTGCCGCGCCGCGGGTGACCGAC
TCCAATAATTTAGTGACTTCTTGCTGTTGTCCTTCAGTAACTTGGCTGGCAACCACTGCTAGTGGTGCAG
GGAGCTGTACGCCCTGAGAACTCACCCCGCCATTTTTATCCTTCAGTTCTCCCGCGACTTGTTGGGTCTG
AAGGGGGGCGGGCGCTGGTGTCTCGACGTAGGGAAGTAGACTTCCAGTTACTGGCGTTGAGCGGTCATGG
GTTATCAACGCACTCA

Protein Sequence : Show Sequence

>YP_068464.1 yopB; Yop targeting protein (plasmid) [Yersinia pseudotuberculosis IP 32953]
MSALITHDRSTPVTGSLLPYVETPAPAPLQTQQVAGELKDKNGGVSSQGVQLPAPLAVVASQVTEGQQQE
VTKLLESVTRGAAGSQLISNYVSVLTKFTLASPDTFEIELGKLVSNLEEVRKDIKIADIQRLHEQNMKKI
EENQEKIKETEENAKQVKKSGIASKIFGWLSAIASVIVGAIMVASGVGAVAGAMMVASGVIGMANMAVKQ
AAEDGLISQEAMKILGPILTAIEVALTVVSTVMTFGGSALKCLANIGAKLGANTASLAAKGAEFSAKVAQ
ISTGISNTVGSAVTKLGGSFAGLTMSHAIRTGSQATQVAVGVGSGITQTINNKKQADLQHNNADLALNKA
DMAALQSIIDRLKEELSHLSESHQQVMELIFQMINAKGDMLHNLAGRPHTV

Molecule Role : Protective antigen
Molecule Role Annotation : Complexes of YopB (pYV0055), YopD, and YopE (BDE) secreted by Yersinia pseudotuberculosis were purified by affinity chromatography and used as immunogens to determine if antibodies to the translocon could provide protection against Y. pestis in mice. The YopB protein or a complex of YopB and YopD (BD) was purified and determined by vaccination to be immunogenic in mice. Mice actively vaccinated with BD or passively vaccinated with anti-BD serum were protected against lethal challenge with F1(-) Y. pestis (Ivanov et al., 2008).

5. yopE

Gene Name : yopE
Sequence Strain (Species/Organism) : Yersinia pseudotuberculosis
NCBI Protein GI : AJJ04959
Other Database IDs : CDD:286154
CDD:119405
Taxonomy ID : 633
Protein Name : outer membrane virulence protein yopE
Protein pI : 6.25
Protein Weight : 22357.55
Protein Length : 314
Protein Note : YopE, N terminal; pfam09020

Protein Sequence : Show Sequence

>AJJ04959.1 outer membrane virulence protein yopE (plasmid) [Yersinia pseudotuberculosis]
MKISSFISTSLPLPTSVSGSSSVGEMSGRSVSQQTSDQYANNLAGRTESPQGSSLASRIIERLSSVAHSV
IGFIQRMFSEGSHKPVVTPAPTPAQMPSPTSFSDSIKQLAAETLPKYMQQLNSLDAEMLQKNHDQFATGS
GPLRGSITQCQGLMQFCGGELQAEASAILNTPVCGIPFSQWGTIGGAASAYVASGVDLTQAANEIKGLAQ
QMQKLLSLM

Molecule Role : Protective antigen
Molecule Role Annotation : A protective epitope in type III effector YopE is a major CD8 T cell antigen during primary infection with Yersinia pseudotuberculosis.(Zhang et al., 2012)

III. Vaccine Information

1. Yersinia pseudotuberculosis phoP mutant vaccine

a. Vaccine Ontology ID:

VO_0002947

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of phoP

Type: Gene mutation
Description: This phoP mutant is from Yersinia pseudotuberculosis IP 32953 (Balada-Llasat et al., 2007).
Detailed Gene Information: Click here.

f. Immunization Route

Oral immunization

g. Mouse Response

Persistence: A phoP mutant is attenuated in mice (Balada-Llasat et al., 2007).
Efficacy: A phoP mutant provided protection against oral challenge with wild type Yersinia pseudotuberculosis (Balada-Llasat et al., 2007).

IV. References

1. Balada-Llasat et al., 2007: Balada-Llasat JM, Panilaitis B, Kaplan D, Mecsas J. Oral inoculation with Type III secretion mutants of Yersinia pseudotuberculosis provides protection from oral, intraperitoneal, or intranasal challenge with virulent Yersinia. Vaccine. 2007; 25(8); 1526-1533. [PubMed: 17194509].

2. Branger et al., 2009: Branger CG, Torres-Escobar A, Sun W, Perry R, Fetherston J, Roland KL, Curtiss R 3rd. Oral vaccination with LcrV from Yersinia pestis KIM delivered by live attenuated Salmonella enterica serovar Typhimurium elicits a protective immune response against challenge with Yersinia pseudotuberculosis and Yersinia enterocolitica. Vaccine. 2009; 27(39); 5363-5370. [PubMed: 19596407].

3. Daniel et al., 2009: Daniel C, Sebbane F, Poiret S, Goudercourt D, Dewulf J, Mullet C, Simonet M, Pot B. Protection against Yersinia pseudotuberculosis infection conferred by a Lactococcus lactis mucosal delivery vector secreting LcrV. Vaccine. 2009; 27(8); 1141-1144. [PubMed: 19135495].

4. Ivanov et al., 2008: Ivanov MI, Noel BL, Rampersaud R, Mena P, Benach JL, Bliska JB. Vaccination of mice with a Yop translocon complex elicits antibodies that are protective against infection with F1- Yersinia pestis. Infection and immunity. 2008; 76(11); 5181-5190. [PubMed: 18765742].

5. Najdenski et al., 2009: Najdenski H, Golkocheva-Markova E, Kussovski V, Vesselinova A, Garbom S, Wolf-Watz H. Attenuation and preserved immunogenic potential of Yersinia pseudotuberculosis mutant strains evidenced in oral pig model. Zoonoses and public health. 2009; 56(4); 157-168. [PubMed: 18793276].

6. Tsybul'ski? et al., 2011: Tsybul'ski? AV, Popov AM, Sanina NM, Maze?ka AN, Portniagina OIu, Novikova OD, Timchenko NF, Kostetski? �Ia. [Immunogenic and protective properties of nanosized constructs based on tubular immunostimulating complexes and pore forming protein of Yersinia pseudotuberculosis]. Zhurnal mikrobiologii, epidemiologii, i immunobiologii. 2011; (2); 43-47. [PubMed: 21598614].

7. Wiki: Y. pseudotuberuclosis: Wiki: Y. pseudotuberuclosis [http://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis]

8. Zhang and Bliska, 2010: Zhang Y, Bliska JB. YopJ-promoted cytotoxicity and systemic colonization are associated with high levels of murine interleukin-18, gamma interferon, and neutrophils in a live vaccine model of Yersinia pseudotuberculosis infection. Infection and immunity. 2010; 78(5); 2329-2341. [PubMed: 20231414].

9. Zhang et al., 2012: Zhang Y, Mena P, Romanov G, Lin JS, Smiley ST, Bliska JB. A protective epitope in type III effector YopE is a major CD8 T cell antigen during primary infection with Yersinia pseudotuberculosis. Infection and immunity. 2012; 80(1); 206-214. [PubMed: 22064714].