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Francisella tularensis

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
    4. Microbial Pathogenesis
    5. Host Ranges and Animal Models
    6. Host Protective Immunity
  2. Vaccine Related Pathogen Genes
    1. FopA (Protective antigen)
    2. FopB (Protective antigen, Protective antigen)
    3. GroEL (Protective antigen)
    4. IglC1 (Protective antigen)
    5. IglC2 (Protective antigen)
    6. LpnA (Protective antigen)
    7. LPS (Protective antigen, Protective antigen)
    8. TUL4 (Protective antigen)
    9. WbtI (Protective antigen, Virmugen)
    10. capB (Virmugen)
    11. clpB (Virmugen)
    12. FTL0552 (Virmugen)
    13. FTT0918 (Virmugen)
    14. galU (Virmugen)
    15. guaA (Virmugen)
    16. guaB (Virmugen)
    17. IglB (Virmugen)
    18. IglC (Virmugen)
    19. PurC/PurD (Virmugen)
    20. purF (Virmugen)
    21. purM (Virmugen)
    22. SodB from F. tularensis SCHU S4 (Virmugen)
    23. sodC (Virmugen)
    24. wbtA (Virmugen)
  3. Vaccine Related Host Genes
    1. Ccl2
    2. Ccl5
    3. Cxcl1
    4. Ifng (Interferon gamma)
    5. IgG
    6. Ighg1
    7. Ighv1-9
    8. IgM
    9. IL-1b
    10. IL-6
    11. Il12a
    12. Il12b
    13. Il4 (interleukin 4)
    14. Nos2
    15. TNF-alpha
  4. Vaccine Information
    1. DeltaFTT0918
    2. F. tularensis FopB Protein Vaccine
    3. F. tularensis GroEL protein vaccine
    4. F. tularensis vaccine LVS LPS
    5. F. tularensis vaccine rLm/iglC
    6. F. tularensis vaccine X4072(pTUIA-15)
    7. Francisella tularensis capB mutant vaccine
    8. Francisella tularensis clpB mutant vaccine
    9. Francisella tularensis FTL0552 mutant vaccine
    10. Francisella tularensis FTT0918 mutant vaccine
    11. Francisella tularensis galU mutant vaccine
    12. Francisella tularensis guaA mutant vaccine
    13. Francisella tularensis guaB mutant vaccine
    14. Francisella tularensis IglB mutant vaccine
    15. Francisella tularensis purF mutant vaccine
    16. Francisella tularensis purMCD mutant vaccine
    17. Francisella tularensis sodC mutant vaccine
    18. Francisella tularensis wbtA mutant vaccine
    19. Francisella tularensis wbtI mutant vaccine
    20. FSC043
    21. J5dLPS/OMP
    22. KKF24
    23. LVS
    24. rLm/iglC
    25. sodB(Ft)
  5. References
I. General Information
1. NCBI Taxonomy ID:
263
2. Disease:
Tularemia
3. Introduction
Francisella tularensis is the etiologic agent of tularemia, a zoonotic disease of which there are several recognized forms depending upon the route of entry of bacteria into the host. These forms include ulceroglandular, glandular, typhoidal, occuloglandular, gastrointestinal and oropharyngeal tularemia. Of these, ulceroglandular tularemia is the most common, with general flu-like symptoms often described. An ulcer at the site of infection is observed, which may persist for up to several months. The lymphatic system disseminates bacteria from the initial infection site and the resultant colonization of the draining lymph nodes leads to swelling; the swollen lymph node resembles the "bubo", a classic symptom of bubonic plague. The most severe form of the disease is the pneumonic form. Primary cases of pneumonic tularemia are generally associated with the disturbance of contaminated dust, for example following the handling of hay or straw which has previously been soiled by infected rodents.

4. Microbial Pathogenesis
There are two types of F. tularensis; Type A is the most virulent causing an often fatal disease while Type B is less virulent and therefore causes a milder form of disease. The species are classified into four distinct sub-species. F. tularensis subspecies tularensis comprises all the strains previously classified as Type A. F. tularensis subspecies holarctica comprises those strains classically referred to as Type B. F. tularensis subspecies mediaasiatica comprises Central Asian strains. The fourth subspecies, F. tularensis subspecies novicida, is a very infrequent cause of human disease, although F. novicida is able to cause a lethal infection in the murine model of disease (Isherwood et al., 2005).

Link to pathogenesis of Francisella tularensis in HazARD.
5. Host Ranges and Animal Models
F. tularensis is the etiologic agent of tularemia, a zoonotic disease that affects a range of animals, including rabbits, hares and rodents. Human disease occurs throughout the northern hemisphere (Isherwood et al., 2005).
6. Host Protective Immunity
While antibody develops against both lipopolysaccharide (LPS) and protein antigens, the role of antibody in protection depends on the nature of the challenge strain. In the murine model of disease, immunization with LPS is able to provide antibody-mediated protection against an otherwise lethal intradermal or intraperitoneal challenge with F. tularensis subspecies holarctica. However, similarly immunized mice are only partially protected against an airborne challenge with F. tularensis subspecies holarctica. When challenged with the most virulent strains of F. tularensis (subspecies tularensis) by any routes, there is at best evidence only of a slight delay in the time to death.

Cellular immunity is likely to play the main role in protection against tularemia. In mice challenged with F. tularensis LVS, neither CD4+ nor CD8+ T cells appear to be absolutely required for control of the infection, but either cell type can promote clearance of the challenge. However, both CD4+ and CD8+ T cells appear to be required for the clearance of a challenge with F. tularensis subspecies tularensis. The clearance of F. tularensis is likely due to the Th1-related cytokines TNFα, IFNγ, and IL-12.

Overall, protection against tularemia is likely to be dependent on the induction of antibody, CD4+ and CD8+ T cell immune responses. The induction of all of these responses appears to be necessary for protection and effective clearance of the most virulent strains (Isherwood et al., 2005).
1. capB
  • Gene Name : capB
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica LVS
  • NCBI Gene ID : 3952402
  • NCBI Protein GI : 89256709
  • Locus Tag : FTL_1416
  • Genbank Accession : AM233362
  • Protein Accession : YP_514071
  • Taxonomy ID : 376619
  • Gene Starting Position : 1344798
  • Gene Ending Position : 1346006
  • Gene Strand (Orientation) : -
  • Protein Name : capsule biosynthesis protein capB
  • Protein pI : 7.41
  • Protein Weight : 41305.26
  • Protein Length : 402
  • DNA Sequence : Show Sequence
    >gi|89255449:1344798-1346006 Francisella tularensis subsp. holarctica LVS chromosome, complete genome
    ACTATAAATTATGCTTCTTTTTATGGCTAGTATCACAGTAATCAACTAATTCCATACCAATATCTTTAAT
    ATTACCAACTCCAACAAGAATATAAGAGTTTGCTGGATTTGAATCAACTGTTTTTTCAAGTATTTGTATA
    GGTGTCATCTCTTCACAAACTATGACTTTAGTCATTGGTTTATTAAGTGATTTTGCATATTTTTTATAAA
    ACGAAGTAAAAACCTCTGTACCAGTACCTATTAGAACAATTAAATCTTGTTTTTGCCAACCTAGAGCTGC
    TTCTGCCATTTGTTTTGAGCGATCCTCTCTATCATCTCTACAGTTAACAACCAATACTTTCTTATCACAG
    CCTGAATACTTAGCACAAAGCTTATCCCAAAGCATTTTTGTTGATACAGGATCATTAGCAGCAAAAGCAT
    TAGCAAAATTTATTTCAGCATTTTTAATATTAAAATTATACTCCGTCATCGCACCTGGATCTGGGGTCGC
    TTCCCACATTCCTTTAAGAGCGATCTCTCTAGGTATACCCAAATCATCTGTTACTTTTAATGCTAGAGCA
    ACATTTATCTTAAATTCTGAGTATACAAACTTATTTATTTCTTCATCAGATATTTTCTCAGCATCTTGTG
    CTGTAGCTGCGATTAGTTCTGAGCCTCGGTCTTTACAAGCATATTCAAAAAAATCTAAATGGATATCTTC
    CGCAGTAAAATACTTAGCTCCAACAGGGATAGTAGCTGCTAATGCTTTTGCAACATCTCTTTCTGTAGGA
    CCCATAACATCTAAATGATCAGGACGAGCATTTGTCAAAACTCCATGTGTCGCTTTAATAAGCTTTAACT
    CACACAGCGATTGCAAAAGTGGCTGCAAAGCCATACACTCGATAACGATAGCATCAGCTTTTGCTCTTCT
    TGCCTTAAACATAATCTTAACTTGCTCAGCAATATTACTAAAACCTATTCTAAATACAGGTGTTTCTGAA
    CCATCAACATCAATATATCTTGCTAAAGTTCCAGTAGTCTTAGCTACTGTTCTATATCCTCCAGCTCTAA
    CACCAGCTGCAATAAGTCTCGCAACACTTGACTTACCACGAGTACCATTGACATGAATCCTAATAGGAAT
    ACTTTTAATACTATTATTATGCACTATATTTTCTATTATTAGATAAACGCATAAAATCACAAATACGACG
    ACAATTAACCAAAAATCCA
  • Protein Sequence : Show Sequence
    >gi|89256709|ref|YP_514071.1| capsule biosynthesis protein capB [Francisella tularensis subsp. holarctica LVS]
    MDFWLIVVVFVILCVYLIIENIVHNNSIKSIPIRIHVNGTRGKSSVARLIAAGVRAGGYRTVAKTTGTLA
    RYIDVDGSETPVFRIGFSNIAEQVKIMFKARRAKADAIVIECMALQPLLQSLCELKLIKATHGVLTNARP
    DHLDVMGPTERDVAKALAATIPVGAKYFTAEDIHLDFFEYACKDRGSELIAATAQDAEKISDEEINKFVY
    SEFKINVALALKVTDDLGIPREIALKGMWEATPDPGAMTEYNFNIKNAEINFANAFAANDPVSTKMLWDK
    LCAKYSGCDKKVLVVNCRDDREDRSKQMAEAALGWQKQDLIVLIGTGTEVFTSFYKKYAKSLNKPMTKVI
    VCEEMTPIQILEKTVDSNPANSYILVGVGNIKDIGMELVDYCDTSHKKKHNL
  • Molecule Role : Virmugen
  • Molecule Role Annotation : There was a deletion in gene capB of F. tularensis live vaccine strain. LVS capB, while much less virulent than LVS, induces potent protective immunity against lethal LVS i.n. challenge in mice, comparable to that induced by LVS (Jia et al., 2010).
  • Related Vaccine(s): Francisella tularensis capB mutant vaccine
2. clpB
  • Gene Name : clpB
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
  • NCBI Gene ID : 3192509
  • NCBI Protein GI : 56708764
  • Locus Tag : FTT_1769c
  • Genbank Accession : AJ749949
  • Protein Accession : YP_170660
  • 3D structure: PDB ID : 3P2L
  • Taxonomy ID : 177416
  • Gene Starting Position : 1857727
  • Gene Ending Position : 1860306
  • Gene Strand (Orientation) : -
  • Protein Name : ClpB protein
  • Protein pI : 5.27
  • Protein Weight : 89876.23
  • Protein Length : 859
  • Protein Note : Similar to FASTA: AAO89660 (Q83F55) ClpB protein from Coxiella burnetii (859 aa). FASTA: opt: 3593 Z-score: 3223.6 E(): 1.1e-171 Smith-Waterman score: 3593; 65.357 identity in 840 aa overlap
  • DNA Sequence : Show Sequence
    >gi|255961454:1857727-1860306 Francisella tularensis subsp. tularensis SCHU S4, complete genome
    TTTATTTAGAGAATATAATATTGTTATTAGCGTCAATATCGACAACTATTTTATCTTCAGCTTTAAACTC
    ACCATCTAGAAGTTTTAGAGCCAGAGGGTTTTCTAAGTTGTTTTGAATAGCGCGCTTAAGCGGTCTAGCA
    CCAAACACAGGATCAAAGCCAGCATCAGCTAGTTTATCCATAGCTCCAGTTGTGACTTCTAATCCTATAC
    TTAGATCTGCTAGACGTTTTTCTAAGCGTTTGATTTGTATTTTAGCTATTTCAGTTATCATCTCTTTGTT
    TAGAGGTTCAAAGACAATTGCATCATCAACTCTATTTACAAATTCAGGTCTAAAGTGGCTAAGTACCATT
    TCCATTACAGCAGATTTTACTGTTTCATAATCCTGACCTTGCATTTCTTGGATTCGATGTGAACCAAGGT
    TAGAAGTCATAACTATTACAGTATTTTTAAAGTCTACTGTTCTGCCTTGCCCATCTGTTAGGCGACCATC
    GTCAAGCACTTGTAGTAAGATGTTAAATATATCAGCATGAGCTTTTTCAACCTCATCAAGTAAGATTACA
    GAATAAGGTTTTCTTCTAACATGTTCAGTTAGATAGCCGCCTTGTTCATAACCTACGTATCCAGGAGGTG
    CGCCTATTAGTCTAGCTACAGAATGTTTCTCCATAAACTCAGACATATCTACTCTAAGCATGGCATCTTC
    ATCATCAAACAAAAACTCTGCTAAAGCTTTTGTAAGCTCAGTTTTACCGACACCAGTTGGACCTAAGAAC
    ATGAATGAGCCTATAGGTCTATTTGGATCTGATAATCCAGAACGAGATCTTCTTACAGCATTTGATACTG
    CTTTTATAGCTTGATCTTGTCCGATTACTCTTTTATGTAAGAAACTTTCCATATTTAGAAGCTTGTCTTT
    TTCGCCTTCCATCATCTTAGACACAGGTATTCCAGTAGCTTTTGAAACTACATCAGCAATCTCATTTTCT
    GTAACAGATGTTCTTACTAATTTGTTTTCAGAAGGTTCTGCTTCAGTTTCTTCTATTTGTTTAATTTGTG
    CTTCTAGCTCAGGTATTTTACCGTATTGTAATTCTGCCATTTTGCTCAAATCACCCACTCTTTGGTACTT
    TTCAAGTTCAAACTTGGCTTTCTCAAGCTCTTCTTTTAGTTTACTTGTACCTTGCATCTTAAGCTTTTCA
    GCTTTCCAAAGCTCTTCTAGTCCTTTATACTCGGAGTCTAGCCCTTTTATTTCTTGCTCAAGTATCTCTA
    AACGTTTTTTAGTAGCATCATCTTTCTCTTTCTTTAGCTGTTCGCGTTGCATTTTCAGCTGGATGATTCT
    ACGATATAAGCTTTCCATTTTTTCAGGTTTAGAGTCTATTTCCATACGAATTTGGCTTGCTGCTTCATCT
    ACTAGATCTATAGCTTTATCAGGTAGCTGTCTATCTGTGATATACCTATGTGACAAAGTTGCTGCACTTA
    CAATAGCTGAATCTGTGATATTTACACCATGATGTAGCTCATATCTTTCTTTTAGGCCTCTAAGTATAGC
    GATAGTATCTTCTACAGTAGGTTCATCAACTAGCACTTTCTGGAATCTTCGCTCAAGTGCCGGATCTTTT
    TCAACATACTCACGATACTCATCTAAAGTTGTTGCACCAACACACTTTAGCTCTCCTCTAGCTAGAGCAG
    GTTTAAGCATGTTACCAGCATCCATAGATCCTTCTGCTTTACCAGCACCTACCATAGTATGTAATTCATC
    TATAAAGAGAATTACATTACCTTCTTGTTTTGATAACTCTTTTAATACAGATTTTAGACGCTCTTCAAAA
    TCTCCTCTAAATTTAGCACCAGCTAGCAGTGCACCCATATCTAATGATAGTACTTTTTTACCTTTGACAC
    CTTCTGGTACTTCATCATTAACTATTCGTTGAGCTAAGCCCTCAACAATAGCAGTTTTACCAACACCAGG
    CTCACCTATAAGCACAGGGTTGTTCTTAGTTCTTCTTTGTAATACTTGAATAGTTCTACGGATCTCACTA
    TCTCTACCGATTATTGGATCAATTTTTCCTTTTCTCGCTAGATCAGTTAGATCTACCGTGTATTTATCTA
    ATGCACCTTTCATATCTTCTTGATTTTGACTACTCACTTTCTCCCCTCCGCGATAATCATTGACTGCTTT
    TGTTAATTTTTCTTTTGTAATACCAAATTTGTTATACAGTCCAGTTAAACTTTTGTCTTCTAAAGAGGCT
    AATAAGAAAACCTCACTCGAGATAAACTCATCACCATTTTTATTAGCAAGCCCTTGCATTTTATGTAATG
    TAGCTATTAAATCTCTAGATGGTGTTACTTGAGGGTTACCTTCTCCAGATAATACCGCAACACTATCAAC
    CATATCATTTACAGCTTTTATAAAGTTTTGTATATTAACACCACAAACGCTTAATATAGATATAGCAACA
    CTATCATTTTGCTCCAAAAGAGCTTTTAGTATATGTGCTGATGTAAACTCAGTTGCTTTTTGTTGAAAAG
    CATAAGATTGAGCCTCAGCTAGAGCTTCTTGTAGTTTTATTGTAAATTTATTTATATCCA
  • Protein Sequence : Show Sequence
    >gi|56708764|ref|YP_170660.1| ClpB protein [Francisella tularensis subsp. tularensis SCHU S4]
    MDINKFTIKLQEALAEAQSYAFQQKATEFTSAHILKALLEQNDSVAISILSVCGVNIQNFIKAVNDMVDS
    VAVLSGEGNPQVTPSRDLIATLHKMQGLANKNGDEFISSEVFLLASLEDKSLTGLYNKFGITKEKLTKAV
    NDYRGGEKVSSQNQEDMKGALDKYTVDLTDLARKGKIDPIIGRDSEIRRTIQVLQRRTKNNPVLIGEPGV
    GKTAIVEGLAQRIVNDEVPEGVKGKKVLSLDMGALLAGAKFRGDFEERLKSVLKELSKQEGNVILFIDEL
    HTMVGAGKAEGSMDAGNMLKPALARGELKCVGATTLDEYREYVEKDPALERRFQKVLVDEPTVEDTIAIL
    RGLKERYELHHGVNITDSAIVSAATLSHRYITDRQLPDKAIDLVDEAASQIRMEIDSKPEKMESLYRRII
    QLKMQREQLKKEKDDATKKRLEILEQEIKGLDSEYKGLEELWKAEKLKMQGTSKLKEELEKAKFELEKYQ
    RVGDLSKMAELQYGKIPELEAQIKQIEETEAEPSENKLVRTSVTENEIADVVSKATGIPVSKMMEGEKDK
    LLNMESFLHKRVIGQDQAIKAVSNAVRRSRSGLSDPNRPIGSFMFLGPTGVGKTELTKALAEFLFDDEDA
    MLRVDMSEFMEKHSVARLIGAPPGYVGYEQGGYLTEHVRRKPYSVILLDEVEKAHADIFNILLQVLDDGR
    LTDGQGRTVDFKNTVIVMTSNLGSHRIQEMQGQDYETVKSAVMEMVLSHFRPEFVNRVDDAIVFEPLNKE
    MITEIAKIQIKRLEKRLADLSIGLEVTTGAMDKLADAGFDPVFGARPLKRAIQNNLENPLALKLLDGEFK
    AEDKIVVDIDANNNIIFSK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A clpB mutant in Francisella tularensis subspecies tularensis strain SCHU S4 was attenuated and provided protection against aerosol challenge in mice (Conlan et al., 2010).
  • Related Vaccine(s): Francisella tularensis clpB mutant vaccine
3. FopA
  • Gene Name : FopA
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica OSU18
  • VO ID : VO_0011078
  • NCBI Gene ID : 4306351
  • NCBI Protein GI : 115315051
  • Locus Tag : FTH_1293
  • Genbank Accession : CP000437
  • Protein Accession : YP_763774
  • Taxonomy ID : 393011
  • Gene Starting Position : 1267837
  • Gene Ending Position : 1269018
  • Gene Strand (Orientation) : -
  • Protein Name : outer membrane protein FopA
  • Protein pI : 5.43
  • Protein Weight : 39079.73
  • Protein Length : 393
  • DNA Sequence : Show Sequence
    >gi|115313981:1267837-1269018 Francisella tularensis subsp. holarctica OSU18, complete genome
    ATTAGTTAGCTTCTTTAAGTGGAGCTGATACGCTAGCTTCTACACGCTGGTTACGCGGATCAGACTTATG
    AATACCACCTAAAGTATCATTATAGCCAAAACCTTTAGTAATTATTTTCTCACTATCTAAACCTAATTGC
    TTCATGTAATCAGCAACAGCTTGTGCTCTCTTCTCAGAAAGTTTTTGGTTATATATATCAAACTCACTAC
    CAGTTTGACCTTGAGAAGCATAACCTTTAACTGTTACAGAGCTAATGTTACTATCCTTGATAAAATTAAC
    AAAAGATGCAATAGCTTTTTTACCTTTAGCATTTAAAGTAGCTTTATCATAAGCAAACTTAGTATCTAGG
    TAAACTGTTACATCATCACCATTTACTGTATAGCACGCGACACCATCTTCAGTTAGATTAAAGTTGCCTT
    CACATTGCTTAATACCAGCAGGTAAAACATACTTAGACTCATCAATAGTTGGCATAGCAACAGTTTGAGC
    AGCTGTAGTCGCACCATTATCCTGAATATTACCAGTGTCATTATTAGTAGTATCTTTGCCACCAAAGAAC
    CATGTTAAACCAGCACCAATCATGTTAGTACCCGCTCTGCCATTAGCACCAGAAATATTACTAGGTGCCA
    TTGTTTGGATATATCTGTAGTCAACACTTGCTTGAACATTTCTAGATAGTTCAAACTTAAGACCACCACC
    CACATCCCAAGCACCTGTTGCTTGACCTGCTAGATTTGCCCAACCAGCACCACCTGCAGCATATGGAGTA
    AACATAGTATTATTACTAAAGTTTACAACACCTTCACCTAAACCAGCAAATACTCTACCAACTAATTGGT
    TGTACTGTACAGCGAAGTATTTATTGATGTTATAACCGATTGTGAAGTTAGCACCAGCACCTGATGGAGA
    GTTAGGAGTTCCAGCTAGACCGTTAGCATCTACACCTAAGTACCACTGGCCAGTTCTATCTTGAGGACCC
    CAAGTATTGTCCTTGTTAGTCAAAGCGCTATAAGTATTTGCAAAAGGAGCAATGAAATTATTAGCTGCAA
    AACCACTGCTTTGTGTAGTAGCTGAATTAGAGTTTGCTGACGTATCGATATTATCTGAACCTGCAGCGAT
    AGATGCTGTAGCTGAACCTAATAATACAGTTGTAGCTATAACAATACTTTTTAATCTCATCA
  • Protein Sequence : Show Sequence
    >gi|115315051|ref|YP_763774.1| outer membrane protein FopA [Francisella tularensis subsp. holarctica OSU18]
    MMRLKSIVIATTVLLGSATASIAAGSDNIDTSANSNSATTQSSGFAANNFIAPFANTYSALTNKDNTWGP
    QDRTGQWYLGVDANGLAGTPNSPSGAGANFTIGYNINKYFAVQYNQLVGRVFAGLGEGVVNFSNNTMFTP
    YAAGGAGWANLAGQATGAWDVGGGLKFELSRNVQASVDYRYIQTMAPSNISGANGRAGTNMIGAGLTWFF
    GGKDTTNNDTGNIQDNGATTAAQTVAMPTIDESKYVLPAGIKQCEGNFNLTEDGVACYTVNGDDVTVYLD
    TKFAYDKATLNAKGKKAIASFVNFIKDSNISSVTVKGYASQGQTGSEFDIYNQKLSEKRAQAVADYMKQL
    GLDSEKIITKGFGYNDTLGGIHKSDPRNQRVEASVSAPLKEAN
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Anti-FopA MAbs protected 90% of naïve C3H/HeN mice from lethal challenge with F. tularensis LVS when administered prophylactically as well as therapeutically (Savitt et al., 2009).
4. FopB
  • Gene Name : FopB
  • Sequence Strain (Species/Organism) : F. tularensis subsp. novicida U112
  • VO ID : VO_0011070
  • NCBI Gene ID : 4547777
  • NCBI Protein GI : 118496734
  • Locus Tag : FTN_0119
  • Genbank Accession : CP000439
  • Protein Accession : YP_897784
  • Taxonomy ID : 401614
  • Gene Starting Position : 130543
  • Gene Ending Position : 131082
  • Gene Strand (Orientation) : +
  • Protein Name : outer membrane protein of unknown function
  • Protein pI : 4.55
  • Protein Weight : 18246.3
  • Protein Length : 179
  • DNA Sequence : Show Sequence
    >gi|118496615:130543-131082 Francisella tularensis subsp. novicida U112, complete genome
    TATGAAAAAAACTATACTAGGAGCAATGATCGCTGGTGGTTTGATGGTTTCTGCAACTACAGCTATGGCT
    GGTGGGGTAGGTTTTGCTAATGTCCAAGATATTTTTGAAACTTCACCTCTTGGTAAAGCAAAGGTAACAG
    CTGATGAGAAAAAGCTAAAGCCACAGATGGACCAACTTAAGCAAAACATCACTGCTTTACAAGAGAAAGT
    AAATGCATACACTCAAGAAAAAGATGATGTTCAAACAGATGATTCTAAAGGCGATACTAAAGATGCACAG
    TCAGCAGACAAAGTAGAAAATCAAGATAAGCAACAAGCTCAAGCAGATCTTGAAAAAGCAATGAAAGATT
    ACCAAAACCTAATGAATCAGGTTCAAAAAATGGCTTCTGACGATGCTGATGCGTTCAAAGATGCTTTGAC
    TAAGGCTTCTGCTCAAGTTGCTAAAGAGAAGCAACTTGATGCTATTTTACCTGCTGAAATGAGTCTATAT
    AATGTTGATAGTATTGATGTTACTAAAGATGTTATAGCTAAGATGCAATA
  • Protein Sequence : Show Sequence
    >gi|118496734|ref|YP_897784.1| outer membrane protein of unknown function [Francisella tularensis subsp. novicida U112]
    MKKTILGAMIAGGLMVSATTAMAGGVGFANVQDIFETSPLGKAKVTADEKKLKPQMDQLKQNITALQEKV
    NAYTQEKDDVQTDDSKGDTKDAQSADKVENQDKQQAQADLEKAMKDYQNLMNQVQKMASDDADAFKDALT
    KASAQVAKEKQLDAILPAEMSLYNVDSIDVTKDVIAKMQ
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Intranasal immunization of HLA-DR4 tg mice with the single antigen FopB from F. tularensis subsp. novicida conferred significant protection against lethal pulmonary challenge with an F. tularensis subsp. holarctica live vaccine strain (Yu et al., 2010).
  • Additional Molecule Role : Protective antigen
  • Related Vaccine(s): F. tularensis FopB Protein Vaccine
5. FTL0552
  • Gene Name : FTL0552
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica LVS
  • NCBI Gene ID : 3952730
  • NCBI Protein GI : 89255956
  • Locus Tag : FTL_0552
  • Genbank Accession : AM233362
  • Protein Accession : YP_513318
  • Taxonomy ID : 376619
  • Gene Starting Position : 534315
  • Gene Ending Position : 535001
  • Gene Strand (Orientation) : ?
  • Protein Name : two-component response regulator
  • Protein pI : 6.31
  • Protein Weight : 23733.73
  • Protein Length : 228
  • DNA Sequence : Show Sequence
    >gi|89255449:534315-535001 Francisella tularensis subsp. holarctica LVS chromosome, complete genome
    TATGAGAATATTGTTGGCTGAAGATGATCTTCATTTGGGTGAAGGCTTATTAGAAGCTTTGCAAAAAGAG
    GGTTTGATTGTAAATCTAGTTTCAGATGGTGAGGCAGCGCAAACTTTTATAGAATCTGGATTGTATGATA
    TAGTCGTCTTAGATATTGGTATGCCAATAAAAACTGGTTTAGAAGTTCTAAGAAATATTAGAAATAGAGG
    TATTAAAGTACCAATAATACTGTTGACTGCTAGAGATGGTTTAGAAGATAGAATTAAAGGTCTTGATCTT
    GGTGCTGATGATTATCTAACTAAGCCATTTGAGTTAAAAGAGCTAGTAGCTAGAATTAAAGCTATTTCTC
    GTCGTATTGATACTAGATCTGGTAAAAGTGTTAATGAAGAAATTAGATTTGGAGATTATAGTTTTAATCC
    AAGCTCTGAAACGGTAACCAAAGATGGAGTTATTATTCCTGTTTCAAAAAAAGAGTTAGCGCTTTTAGCT
    ATATTGGTACAAAATGCTGGTAGGGTAGTACCAAAGACACAGCTTTTAGAAGAAATATATTCTACTGATA
    AGGAAATGGATACAAACACCCTAGAAGTACATATGCATAATTTAAGAAAGAAAATTAACATTCCTAATTT
    TATACAGACTATTAGAGGTGTTGGTTACTTTGTACAAAAGGATAAAGTAATTAAGTA
  • Protein Sequence : Show Sequence
    >gi|89255956|ref|YP_513318.1| two-component response regulator [Francisella tularensis subsp. holarctica LVS]
    MRILLAEDDLHLGEGLLEALQKEGLIVNLVSDGEAAQTFIESGLYDIVVLDIGMPIKTGLEVLRNIRNRG
    IKVPIILLTARDGLEDRIKGLDLGADDYLTKPFELKELVARIKAISRRIDTRSGKSVNEEIRFGDYSFNP
    SSETVTKDGVIIPVSKKELALLAILVQNAGRVVPKTQLLEEIYSTDKEMDTNTLEVHMHNLRKKINIPNF
    IQTIRGVGYFVQKDKVIK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A FTL0552 mutant in Francisella tularensis was attenuated and provided partial protection in mice against challenge with a lethal dose of the virulent SchuS4 strain when compared to naive mice (Sammons-Jackson et al., 2008).
  • Related Vaccine(s): Francisella tularensis FTL0552 mutant vaccine
6. FTT0918
  • Gene Name : FTT0918
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
  • NCBI Gene ID : 3191684
  • NCBI Protein GI : 56708019
  • Locus Tag : FTT_0918
  • Genbank Accession : AJ749949
  • Protein Accession : YP_169915
  • Taxonomy ID : 177416
  • Gene Starting Position : 927665
  • Gene Ending Position : 929338
  • Gene Strand (Orientation) : ?
  • Protein Name : hypothetical protein
  • Protein pI : 4.53
  • Protein Weight : 55767.38
  • Protein Length : 557
  • Protein Note : ORF ftt0918
  • DNA Sequence : Show Sequence
    >gi|255961454:927665-929338 Francisella tularensis subsp. tularensis SCHU S4, complete genome
    TGTGGTGCGTAAATTTAAAAAAACCTGTTTGATAGTTAGTAGTTTATTGGCTTGTAGTGGTTTAGCTTAT
    TCTGAAGATTCTCCTCAAGTTGTTTCACAAGGGGGGCCTCTTGGAGCTACTAGTATTGGTGATCAAAACC
    TTGGACAGCCAGATCCAAATGCTAGTGGAGCCTCTTCAACAACACAGACTACCGGTTCAAATCTAAATGA
    TAGGGAACTTTTGCTAAAATTACAGCAGCAGGTACAGCAACTTCAGGGACAATTACAACAGCTAAAAGCA
    CAGGGTAATGGTGGTGGATTACAGAATACCTATAATGGTAGTTCGCAGTTTACTACTTACAGCTCAAAAG
    TTGATGGTAATAAAAATCCTCGTACGCTTGGAGGCAATGGTGAGAGTAAAGATCTGAGTCAGGCTTTGAT
    TGGTGGTCAAACGTCGTCAGATATTATGGGGAATGTTAATGCTAGTAACTCTATCATTAATTTAGCTTCT
    GAGCCATTAGGAGGCGTCTTTAACCAAAAAGGCGGTATCGACGTTGGTGGAGCTCCGGCGATTACAACAC
    AAGGTCAAGTTACCTACTTAGGTTCGTACTCTGGTAACAACAGTATTCCAATTGGTCAGATTTCTTCTAA
    CCTTTTTGCTTCTACATTGTTGGGTCAAAGAGAGAAGTTTGATGACTACTCTGTATTCTTTGGTGGCTTT
    ATAGAAGCAGATGCCCAAGCTTGGTTTGGTAGTGCTGTTACTAAGGTGCAAAATGCTGGCCAGTTATCTA
    GCAATGGCCAAAATATATATTTAACATCAGCTAATTTATATTTCTTATCAAATCTTGGTCATTATGTAAC
    AGCTCAGTTTGATTTTGATACTAATGAGTCAGGAAGTTTTAGTTTAGGTAATGCTTTTGTAATTTTTGGT
    AACTTAGATATATCACCATTCTTCGTAACAGCAGGTAGAAACAAGCTATCTGTTGGCTCATATGGTGGTG
    GTGGTACTTGGACTAGCGGTATCACCAAATTTCTATCACCAAATCAGGTTACTAACGTATCTATTGACTA
    TAAAGATCAAGTCTGGAACGCCAACATTGCAGTATTTGGCTCTGATGATAGACGTGCAAACTTCTCAACA
    GGTTTATTCTATGCTGATAGCTGGACACCAAACTTAGCGGCTGGTTTTAACGTAGGTTATGTCTTTAATA
    TTGCTGGTGCTGGTAACTCTTCGATTGCTAACTCATTAGCTAACTTAAATCGTAGTAGTGATAATGTGGG
    AGCTTTAAACGTTGACGGCAACTTAACTTATGCAATTTGGGATGGATTTTTAAACTTAGGAGCAGGTTGG
    GCTAGTACTACGACAAAAGAAGATTTTAATAATAATGGTGGTAGTGTACTTGCTGGGGCATGGTATGGAG
    CACTTAACTATTCTGCGATACTTGGTGGTAGAAATACTAACTTCGGTGTGACTTATGGTCAATCATATAA
    TGCTGCAGCTATCCCAATGGAGACAGCAAATGCTTCACCAACTTTCGGTCAAACAGCATCTGGTATCAAA
    CAGCAACTTATCTTCTCGGCTCAGCGAGCTTACTTTGATGACAATGTTCTATTTGGTCCTGAATATGCGT
    ATCAAAGACTATATACTGGCGAACATATGAATACAATTACTCTGGATATGTCGGTATACGTATA
  • Protein Sequence : Show Sequence
    >gi|56708019|ref|YP_169915.1| hypothetical protein FTT_0918 [Francisella tularensis subsp. tularensis SCHU S4]
    MVRKFKKTCLIVSSLLACSGLAYSEDSPQVVSQGGPLGATSIGDQNLGQPDPNASGASSTTQTTGSNLND
    RELLLKLQQQVQQLQGQLQQLKAQGNGGGLQNTYNGSSQFTTYSSKVDGNKNPRTLGGNGESKDLSQALI
    GGQTSSDIMGNVNASNSIINLASEPLGGVFNQKGGIDVGGAPAITTQGQVTYLGSYSGNNSIPIGQISSN
    LFASTLLGQREKFDDYSVFFGGFIEADAQAWFGSAVTKVQNAGQLSSNGQNIYLTSANLYFLSNLGHYVT
    AQFDFDTNESGSFSLGNAFVIFGNLDISPFFVTAGRNKLSVGSYGGGGTWTSGITKFLSPNQVTNVSIDY
    KDQVWNANIAVFGSDDRRANFSTGLFYADSWTPNLAAGFNVGYVFNIAGAGNSSIANSLANLNRSSDNVG
    ALNVDGNLTYAIWDGFLNLGAGWASTTTKEDFNNNGGSVLAGAWYGALNYSAILGGRNTNFGVTYGQSYN
    AAAIPMETANASPTFGQTASGIKQQLIFSAQRAYFDDNVLFGPEYAYQRLYTGEHMNTITLDMSVYV
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An FTT0918 mutant showed significantly reduced virulence in mice. Mice that recovered from infection with the mutant were protected 2 months later from challenge with wild type F. tularensis (Twine et al., 2005).
  • Related Vaccine(s): Francisella tularensis FTT0918 mutant vaccine
7. galU
  • Gene Name : galU
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
  • NCBI Gene ID : 3191600
  • NCBI Protein GI : 56707872
  • Locus Tag : FTT_0757
  • Genbank Accession : AJ749949
  • Protein Accession : YP_169768
  • Taxonomy ID : 177416
  • Gene Starting Position : 776923
  • Gene Ending Position : 777786
  • Gene Strand (Orientation) : ?
  • Protein Name : UTP--glucose-1-phosphate uridylyltransferase
  • Protein pI : 7
  • Protein Weight : 30262.51
  • Protein Length : 287
  • Protein Note : Similar to Q9K0G7 UTP--glucose-1-phosphate uridylyltransferase from Neisseria meningitidis (serogroup B) (289 aa). FASTA: opt: 1017 Z-score: 1291.1 E(): 5e-64 Smith-Waterman score: 1017; 57.439 identity in 289 aa overlap.
  • DNA Sequence : Show Sequence
    >gi|255961454:776923-777786 Francisella tularensis subsp. tularensis SCHU S4, complete genome
    AATGAAAATAAGAAAAGCAGTTTTTCCTGTAGCTGGATGGGGCACTAGATTTTTACCAGCTACTAAATCA
    TGTCCTAAAGAAATGCTAACAGTTGTTGATAAACCATTGATCCAATATGCTGTTGAAGAGGCTATAGAGG
    CAGGCTGTAAAGAGATAATATTTGTTACTAGCTCAAATAAAAAATCCCTAGAAGATCATTTTGATAGAAA
    CTTTGAATTAGAATATTCGCTAGAAAAAAAACAAAAATATGAATTGTTAGATTTAGTAAAAAATATAATT
    CCTAAAGATGTAAGTTTTTTCTTTGTACGTCAGCCAGAGGCGCTAGGACTAGGGCATGCGGTTTTATGTG
    CAAAACCACTAGTTGGAATTGAGGATTTTGCAGTTATATTACCTGATGATTTAATTTACAATCATGATTG
    TGGTACTGGTACGCTAAAGCAAATGGTTAAGGCGGTAGAAGGTACTGATATTAGAGGTTGTATCGCAACT
    CAACAAGTCAAGAGAGAAAAAACAAATTCATATGGTATAGTTGCAAAAGATAATGATAATCTAATCAAAG
    CAATTGTTGAAAAGCCAGCACCAGAAAAAGCACCATCAACTAATGCAGTTGTGGGTAGATATTTATTACC
    AAATAAGATTTTTAGATGTTTAGAATCAACTTCAGAGGGCGCAGGTGGTGAGATTCAACTAACTGATGCT
    ATCGCTAAATTATTAGATCAGGATGAGAAAATTTTGTCTTATGAATTTAAGGGTACTCGTTATGATTGTG
    GCAGTAAATTAGGTTTTTTAATTGCTAATTATGAGATAGCTTTACAGCATCAAGAGTTAGGACATAAATT
    CAAAGAATACCTTCAAAATAGATA
  • Protein Sequence : Show Sequence
    >gi|56707872|ref|YP_169768.1| UTP--glucose-1-phosphate uridylyltransferase [Francisella tularensis subsp. tularensis SCHU S4]
    MKIRKAVFPVAGWGTRFLPATKSCPKEMLTVVDKPLIQYAVEEAIEAGCKEIIFVTSSNKKSLEDHFDRN
    FELEYSLEKKQKYELLDLVKNIIPKDVSFFFVRQPEALGLGHAVLCAKPLVGIEDFAVILPDDLIYNHDC
    GTGTLKQMVKAVEGTDIRGCIATQQVKREKTNSYGIVAKDNDNLIKAIVEKPAPEKAPSTNAVVGRYLLP
    NKIFRCLESTSEGAGGEIQLTDAIAKLLDQDEKILSYEFKGTRYDCGSKLGFLIANYEIALQHQELGHKF
    KEYLQNR
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A galU mutant is attenuated in mice, with only transient weight loss observed. Two months after inoculation, mice were challenge with wild type F. tularensis and were protected, once again only experiencing transient weight loss (Jayakar et al., 2011).
  • Related Vaccine(s): Francisella tularensis galU mutant vaccine
8. GroEL
  • Gene Name : GroEL
  • Sequence Strain (Species/Organism) : Francisella tularensis
  • VO ID : VO_0011075
  • NCBI Protein GI : 282159959
  • Other Database IDs : CDD:171768
    CDD:48161
  • Taxonomy ID : 510831
  • Gene Strand (Orientation) : ?
  • Protein Name : chaperonin GroEL
  • Protein Length : 544
  • Protein Note : chaperonin GroEL; Reviewed; PRK12849
  • Protein Sequence : Show Sequence
    >gi|282159959|gb|ADA79350.1| chaperonin GroEL [Francisella tularensis subsp. tularensis NE061598]
    MAAKQVLFSDEARAKMLDGVNTLANAVKVTLGPKGRNVVLDKSFGTPTITKDGVSVAKEIELEDKFENMG
    AQIVKEVASKTADVAGDGTTTATVLAQALLTEGLKAVAAGMNPMDLKRGIDKATARLVEELKALSKPCSD
    PKSIEQVGTISANSDATVGKLIADAMAKVGKEGVITVEEGKGFEDELDVVEGMQFDRGYLSPYFATNQEN
    MTTDLENPYILIVDKKISNIRDLLPILEGVSKSGRALLIIAEDVESEALATLVVNNMRGVVKVCAVKAPG
    FGDRRKAMLEDIATLTGATFVSEDLSMKLEETNMEHLGTASRVQVTKDNTTIIDGAGEKEAIAKRINVIK
    ANIAEANSDYDREKLQERLAKLSGGVAVIKVGAVTEAEMKEKKDRVDDALHATRAAVEEGIVAGGGVALI
    RAQKALDGLTGENDDQNYGIALLRKAIEAPLRQIVSNAGGESSVVVNQVKANQGNYGYNAANDTYGDMVE
    MGILDPTKVTRSALQHAASIAGLMITTEAMIGEIKEAAPAMPMGGGMGGMPGMM
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Researchers have shown that mice that had been immunized with purified heat shock protein 60 (Hsp60, groEL) isolated from Francisella tularensis were protected against a subsequent challenge with some strains of the bacterium (Hartley et al., 2004).
  • Related Vaccine(s): F. tularensis GroEL protein vaccine
9. guaA
  • Gene Name : guaA
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica LVS
  • NCBI Gene ID : 3951701
  • NCBI Protein GI : 89256406
  • Locus Tag : FTL_1071
  • Genbank Accession : AM233362
  • Protein Accession : YP_513768
  • Taxonomy ID : 376619
  • Gene Starting Position : 1023478
  • Gene Ending Position : 1025028
  • Gene Strand (Orientation) : ?
  • Protein Name : GMP synthase
  • Protein pI : 6.04
  • Protein Weight : 52837.22
  • Protein Length : 516
  • Protein Note : contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway
  • DNA Sequence : Show Sequence
    >gi|89255449:1023478-1025028 Francisella tularensis subsp. holarctica LVS chromosome, complete genome
    AATGACAGATATACATAATCATAAGATTTTGATTTTAGATTTTGGCTCGCAATATACACAACTTATTGCT
    CGTAGAGTAAGAGAAGTAGGTGTTTTTTGTGAAATTTTCCCTCATGATGTAGCAGCTGATTTTATCAAAA
    ATTATCAAGCAAAAGGAATAATTTTATCAGGTGGTCCTGAGTCAGTTTATGATTCTGATGTCAAAGCTCC
    TGAGATAGTTTTTGAGCTTGGAGTACCTGTTTTGGGTATTTGTTATGGCATGCAAACAATGGTAATGCAG
    CATGGTGGCGAAGTAAAAGGAGCTGATCAAAGTGAATTTGGTAAGGCAATTATAAATATCCTAAATTTAA
    CAAATAACATATTTTCAAATATGGAGCATGAGCAGCTAGTTTGGATGAGTCATAGTGACAAAGTCACTCA
    AACTGGTGAGCATTTTGAGATAATTGCTTCTTCAACAAATGCTCCAGTTGCGGCAGTTGCACATAAAAAT
    AAGCCTTTTTTTGGTGTGCAGTTTCACCCAGAAACTACTCATACAGAAAATGGTAAGCAAATTATTGAGA
    ATTTTGTCGTTAATATCTGCGGATGTGATACTCTGTGGAATATCGAAAACATTATCGAAAATGATATAAA
    AGAAATTAAACAAAAAGTTGGTACTGATAAAGTAATTTTAGGGCTATCTGGAGGTGTTGATTCATCTGTT
    GTAGCTGCGATATTACATCAAGCTATCGGCGATCAGCTAACTTGTATATTTGTAGATACTGGTTTACTGC
    GCCTTAATGAGGGTGATCAAGTTATGCAAGTATTTGCAGAGCATATGGATATTAATGTTATTCGTATCAA
    TGCAAAAAATAGATTCTTAGATGCCCTAAGGGGTATCTGTGATCCAGAACAAAAACGTAAAATTATCGGT
    AAGCTATTTGTTGATATTTTTGATGAAGAGGCTGCTAAGATAGAGAATGCTAAATGGCTTGCGCAGGGCA
    CTATCTACAGTGATGTAATTGAATCAGCTAGCAATAATCAGTCTAAAGCACATGTAATTAAATCACATCA
    TAATGTTGGTGGCTTACCAAAAGAAATGAAACTTAAGCTTTTAGAACCATTAAGAGAGCTTTTCAAAGAT
    GAGGTTCGTAAATTAGGCTTAGGTTTAGGATTACCTTATAATATGCTTTATAGACATCCATTTCCTGGTC
    CTGGTCTTGGAGTACGTATATTAGGTGAAATAAAGAAAGAGTATGTAGAAACGTTACAAAAAGCTGATGC
    TATATTTACAGAAGAGCTTTATAAACATAATTTATACCATGATGTTTCTCAAGCTTTTGGGGTGTTTTTA
    CCTATCAAATCGGTAGGTGTAGTTGGTGATCAGCGTAGATATGAGTATGTAATAGCTCTAAGAGCTGTCG
    TTAGTATCGATTTTATGACTGCAACGTGGGCTAATTTACCTTATGATTTTCTGTCACTAGTTTCAAATAG
    AATTGTAAATGAAGTAAAACAAGTATCAAGAGTTGTGTATGATGTGACAGGAAAACCACCTGGAACAATT
    GAATGGGAATA
  • Protein Sequence : Show Sequence
    >gi|89256406|ref|YP_513768.1| GMP synthase [Francisella tularensis subsp. holarctica LVS]
    MTDIHNHKILILDFGSQYTQLIARRVREVGVFCEIFPHDVAADFIKNYQAKGIILSGGPESVYDSDVKAP
    EIVFELGVPVLGICYGMQTMVMQHGGEVKGADQSEFGKAIINILNLTNNIFSNMEHEQLVWMSHSDKVTQ
    TGEHFEIIASSTNAPVAAVAHKNKPFFGVQFHPETTHTENGKQIIENFVVNICGCDTLWNIENIIENDIK
    EIKQKVGTDKVILGLSGGVDSSVVAAILHQAIGDQLTCIFVDTGLLRLNEGDQVMQVFAEHMDINVIRIN
    AKNRFLDALRGICDPEQKRKIIGKLFVDIFDEEAAKIENAKWLAQGTIYSDVIESASNNQSKAHVIKSHH
    NVGGLPKEMKLKLLEPLRELFKDEVRKLGLGLGLPYNMLYRHPFPGPGLGVRILGEIKKEYVETLQKADA
    IFTEELYKHNLYHDVSQAFGVFLPIKSVGVVGDQRRYEYVIALRAVVSIDFMTATWANLPYDFLSLVSNR
    IVNEVKQVSRVVYDVTGKPPGTIEWE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaA mutant is highly attenuated in mice and induces significant protection from challenge with parent strain F. tularensis LVS (Santiago et al., 2009).
  • Related Vaccine(s): Francisella tularensis guaA mutant vaccine
10. guaB
  • Gene Name : guaB
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica FTNF002-00
  • NCBI Gene ID : 5523917
  • NCBI Protein GI : 156502929
  • Locus Tag : FTA_1563
  • Genbank Accession : CP000803
  • Protein Accession : YP_001428994
  • Taxonomy ID : 458234
  • Gene Starting Position : 1398915
  • Gene Ending Position : 1400375
  • Gene Strand (Orientation) : -
  • Protein Name : inosine-5'-monophosphate dehydrogenase
  • Protein pI : 7.79
  • Protein Weight : 47330.3
  • Protein Length : 486
  • Protein Note : 1.1.1.205
  • DNA Sequence : Show Sequence
    >gi|156501369:1398915-1400375 Francisella tularensis subsp. holarctica FTNF002-00 chromosome, complete genome
    TTTAAGACTGGTAATTAGGTGGTTCTTTAGTAATCGTGACGTTATGTACATGCGATTCATTAAATCCAGC
    ACCTGTAATTTGTACAAATGTCGGTTCTGTTCGCATTGTTTGGATATCTTTTGAGCCAGTATAGCCCATA
    CTAGATTTCAGACCACCTATTAGCTGATGAATCACTGCAGATAATAAACCTTTGTATGGTACTCTGCCTT
    CAACGCCTTCTGGAACAAACTTTTTCGCCTCTGTTTCACTTTGGAAATATCTATCAGATGAGCCTTTCTC
    CATAGCACCAAGAGAACCCATACCACGATATGATTTATAAGAGCGACCTTGGAAAAGCTCAACCTCCCCT
    GGTGATTCTTCAGTACCTGCAAAAAGCCCTCCAATCATAACTACAGATGCTCCAGCAACTATAGCTTTTG
    CAATATCACCGGAATATCTAATACCGCCATCTGCAATCACAGGCACTCCAGTTCCTTTTAATGCCTCTGC
    AACATTAGCTATCGCTGTAATTTGTGGTACACCAACACCAGCAACAATTCTGGTAGTACAGATTGAGCCA
    GGTCCAATACCAACCTTGACAGCATCAGCACCAGCTTTAACTAGATCTTTAGCAGCTTCTGCTGTAGCGA
    TATTACCACCTATAACCTGAATATGTGGATAGTTATCTTTAACCCACTTAACTGTATCTAACACACCTTG
    TGAATGTCCATGAGCAGTATCTACAACAATAATATCGACTCCTTCAGCTGCCAATGCTGCAACACGCTCT
    TTAGTATTAACAGCGGTTCCAACAGCAGCGCCAACTCTCAAACGCCCAAGAGAATCTTTACAAGCATTTG
    GTTTATTTTGTGATCTTTCGATATCTTTTGTTGTAATAAGACCGACCAACTCGCCTTGTTCATTAACTAC
    AAGCAACTTCTCAATTTTATGCTCATGAAGTTTCTTTTTAATCGCTCCTTGAGAAGCATCTTCAGGCACT
    GTTACAAGTTTTTCTCTAGGTGTCATAATAGAGCTAACAGGTTCATCTAAATCTTTTGCAAATCTAAAAT
    CACGCCTTGTAACAATACCTATGATTTTATTATTATCATCAACAACGGGAAAACCAGAGAAATTATGTTC
    TTTAGCTAGTTGCATGATTTCTTTAATTGAGCTTTCTTGTTTAATGGTAATGGGATCAATCACCATTCCA
    TTTTCAAATCTTTTTACCTTTTTAACTTCTTGGGCTTGGGCTTGAATAGACATATTCTTATGAATAATAC
    CAATACCACCTTCCTGGGCTATCGCAATAGCTAGGCGTGACTCAGTAACCGTATCCATAGCTGCAGATAC
    TAAAGGAATATTTAACTGAATATCTCTAGTTATATTTGTCTTCAAATCAACTTGATGAGGAAGAACATTT
    GAATATCTAGGTGATAGCAATACATCATCAAAAGTGATTGCCTGCTGAGTAATTCTTAACA
  • Protein Sequence : Show Sequence
    >gi|156502929|ref|YP_001428994.1| inosine-5'-monophosphate dehydrogenase [Francisella tularensis subsp. holarctica FTNF002-00]
    MLRITQQAITFDDVLLSPRYSNVLPHQVDLKTNITRDIQLNIPLVSAAMDTVTESRLAIAIAQEGGIGII
    HKNMSIQAQAQEVKKVKRFENGMVIDPITIKQESSIKEIMQLAKEHNFSGFPVVDDNNKIIGIVTRRDFR
    FAKDLDEPVSSIMTPREKLVTVPEDASQGAIKKKLHEHKIEKLLVVNEQGELVGLITTKDIERSQNKPNA
    CKDSLGRLRVGAAVGTAVNTKERVAALAAEGVDIIVVDTAHGHSQGVLDTVKWVKDNYPHIQVIGGNIAT
    AEAAKDLVKAGADAVKVGIGPGSICTTRIVAGVGVPQITAIANVAEALKGTGVPVIADGGIRYSGDIAKA
    IVAGASVVMIGGLFAGTEESPGEVELFQGRSYKSYRGMGSLGAMEKGSSDRYFQSETEAKKFVPEGVEGR
    VPYKGLLSAVIHQLIGGLKSSMGYTGSKDIQTMRTEPTFVQITGAGFNESHVHNVTITKEPPNYQS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaB mutant is attenuated in mice and induces significant protection from challenge with parent strain F. tularensis LVS (Santiago et al., 2009).
  • Related Vaccine(s): Francisella tularensis guaB mutant vaccine
11. IglB
  • Gene Name : IglB
  • Sequence Strain (Species/Organism) : Francisella novicida U112
  • VO ID : VO_0011071
  • NCBI Gene ID : 4548125
  • NCBI Protein GI : 118497903
  • Locus Tag : FTN_1323
  • Genbank Accession : CP000439
  • Protein Accession : YP_898953
  • Taxonomy ID : 401614
  • Gene Starting Position : 1397634
  • Gene Ending Position : 1399154
  • Gene Strand (Orientation) : -
  • Protein Name : intracellular growth locus protein B
  • Protein pI : 4.5
  • Protein Weight : 54019.41
  • Protein Length : 506
  • DNA Sequence : Show Sequence
    >gi|118496615:1397634-1399154 Francisella tularensis subsp. novicida U112, complete genome
    TTTAGTTATTATTTGTACCGAATAATTCTGGTTCAAGCCTAGTATCTATAGTCATTGTAGTATTCATTCC
    TTCAAATTGAATGTGGGGAATTACATTTATTTTGCATGAATACCAGCCAGGCTTACCCGGTATGGTTTTA
    ACCTCGATAGAAACGTTTCTGAAAGGATATCTCGCCATTTCTAAAGGGGTTGGTTGATAGACTGTGGTGA
    CAAATTCTGATATCCAATCAGAAAGAATTTTTTGAATCGACTCGACATCCACAATACTACCAATCTTATC
    TCTAATTACACATTTAATATAATGAGATATTCTCGATATACACATAGTGTAAGAAAGGTTAGCAATTAAT
    CTTGAGTTTGCTGAGTCAAATCCATCTACAAATTCTTCGACTTTTTTAGCAGAATTTACACTAAAGAAAC
    AAGCGTTACTAGTACCTTTTTCACTTACAAATGGTATTAAACCAATATTTGCAAGTGATAACTCCATATA
    ATCAGCAAATAATACATTTAGAGGTGACTTAGTTTCTAGAATGCCTTTATTATCATATACGCAAGCTACC
    AAGTTTTTTACATAACCACCACTCTCAACTCCTCTTATATATTGGAACCATCTAGTTTTATCATAAGATC
    TCATCATATTCTTAACTAGATGAATTGAAGCAGGTCCCCATAGATAACTTTCATTCTTATCATAATCAAC
    AAACTCATTAAAGCCTTCCATAAGTTTATACTGAACTGGATTATTCTCAGGATTATATGGTTGCCGCAAC
    ATAAAATCACCTACGGTCAAACCTATATATGCAGCAACATCAAGGTTTCTAAAATCATTCCACTCTTTAT
    ATCTAGGATGCTCAAGCAAAGCTTCAAAACTTTTTATATGAGTGATTTCTGATAAATCCTTAACACCAAA
    GAATGACTTATCAATTGATGCAATAAATGGTGCATGAGAATTCTTTGCCACCATACCCATTCCAGTCAAC
    CAAATTATATCATTTGTGGTATTTTCAAAATTATACAATCCTAATATTGCGCCATAAGGTTCGCCACCAT
    ATTGATCAAATTCTGAAACATATACTTTTTTGAAAAAGTCACTACTAGATATATCATATAAATTTCTCTC
    GAAATCATATTGTAGCTCTTCTTTTTTTACATCTAATATACTTACTTCAACATTATCATAGTCTTCTTGA
    CAAACCTCTTGCACCTTTAGCCATTCTTGCTCAAGTGCTCTAAACTCATCATTAGATATAATAGAGTTAA
    CTTGTAAATCAATCAGCTTATCAATAACTGTAATAACTTTTTGAATATATTTTTGATTGTAATTATTTAT
    ATTATCATTATTTGCTAATACCAAAGATAGCGCCATAAGGTTTCTAGCATTGTAGTCAGTAGATAAAGAT
    AAAACTTTAGAAGCATCATCTGAAACATCAAAATCTATATTTTTGAGTACACTATCAACTTCTGTAGATC
    CCCCAAAATTATTTAAAAGTTCATCAGTGAGACTTAATTTATTTATTGTCA
  • Protein Sequence : Show Sequence
    >gi|118497903|ref|YP_898953.1| intracellular growth locus protein B [Francisella tularensis subsp. novicida U112]
    MTINKLSLTDELLNNFGGSTEVDSVLKNIDFDVSDDASKVLSLSTDYNARNLMALSLVLANNDNINNYNQ
    KYIQKVITVIDKLIDLQVNSIISNDEFRALEQEWLKVQEVCQEDYDNVEVSILDVKKEELQYDFERNLYD
    ISSSDFFKKVYVSEFDQYGGEPYGAILGLYNFENTTNDIIWLTGMGMVAKNSHAPFIASIDKSFFGVKDL
    SEITHIKSFEALLEHPRYKEWNDFRNLDVAAYIGLTVGDFMLRQPYNPENNPVQYKLMEGFNEFVDYDKN
    ESYLWGPASIHLVKNMMRSYDKTRWFQYIRGVESGGYVKNLVACVYDNKGILETKSPLNVLFADYMELSL
    ANIGLIPFVSEKGTSNACFFSVNSAKKVEEFVDGFDSANSRLIANLSYTMCISRISHYIKCVIRDKIGSI
    VDVESIQKILSDWISEFVTTVYQPTPLEMARYPFRNVSIEVKTIPGKPGWYSCKINVIPHIQFEGMNTTM
    TIDTRLEPELFGTNNN
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Intranasal vaccination with KKF235 (F. tularensis subsp. novicida mutant (DeltaiglB) ) protected mice from subsequent homotypic challenge with U112 as well as heterotypic challenge with F. tularensis subsp. holarctica (LVS). Most significantly, oral immunization with KKF235 protected mice from a highly lethal subsp. tularensis (SCHU S4) pulmonary challenge (Yu et al., 2010).
  • Related Vaccine(s): Francisella tularensis IglB mutant vaccine
12. IglC
  • Gene Name : IglC
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. novicida U112
  • NCBI Gene ID : 4548124
  • Locus Tag : FTN_1322
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence
    >gi|118496615:1396989-1397618 Francisella tularensis subsp. novicida U112, complete genome
    CTATGCAGCTGCAATATATCCTATTTTAGCAACTCCTTTATCTGTGCTAGCTGCGCAACATACTGGCAAG
    CTTCCTTGAGATGTTGTAAGACCATCTATAGATATAATATGATAACTCATTCCATCTTTTGAAACACCCA
    TAAGTTCTGTTGGCTCTATACTAATACTAAAAGCCTTAGCACTTATTGGATATAACTCTAAATTAGATAG
    ATCTATCATAATACCCCATGCTTCATCAGTTTTGTACTCTTGTTTTTTATTAGAATTACCTAACTTAATT
    TTCATATCTGTAGCACTTGCTTGTAATATGCTCGAAACTTTCTCTTCAATTTTAGTTCTGACATTTGATT
    TAATTAGAACACCAAGAGTAATACTTATTGTTGGATTTATAGAGCCTGGCGTTATAGATAAACGTATTAC
    ACTAGCAGCAGCTGTAGCCGAATCAGCTTTCGTGACATCCTCTCCACCCTCTATAGCAACGATATTTTTA
    TCAAGTTTCTCCCCAGCTATAGTTAAAGAATCAATAAATAATCTACAATTAGGATGAGATCCTATACATG
    CAGTAGGATCAGTTCTCACATGAATGGTCTCGCCACTTGTTACCTGTTGTCTTGTTATCATCTCACTCAT
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An iglC mutant is highly attenuated in mice. Inoculated mice were challenged 30 days later with wild type F. tularensis and were highly protected (Pammit et al., 2006).
  • Related Vaccine(s): KKF24
13. IglC1
  • Gene Name : IglC1
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
  • VO ID : VO_0011072
  • NCBI Gene ID : 3191591
  • NCBI Protein GI : 56708413
  • Locus Tag : FTT_1357c
  • Genbank Accession : AJ749949
  • Protein Accession : YP_170309
  • 3D structure: PDB ID : 2QWU
  • Taxonomy ID : 177416
  • Gene Starting Position : 1398637
  • Gene Ending Position : 1399272
  • Gene Strand (Orientation) : -
  • Protein Name : intracellular growth locus, subunit C
  • Protein pI : 6.26
  • Protein Weight : 21340.81
  • Protein Length : 211
  • Protein Note : Similar to AAP58964.1 (Q7X3J5) IglC (23 kDa protein) from Francisella novicida (211 aa). BLAST Score = 405, Expect = e-112 Identities = 209/211 (99), Positives = 210/211 (99) Identical to FTT1712. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625
  • DNA Sequence : Show Sequence
    >gi|255961454:1398637-1399272 Francisella tularensis subsp. tularensis SCHU S4, complete genome
    ACTATGCAGCTGCAATATATCCTATTTTAGCAACTCCTTTATCTGTGCTAGCTGCGCAACATACTGGCAA
    ACTTCCTTGAGATGTTGTAAGACCATCTATAGATATAATATGATATCTCATTCCATCTTTTGAAACACCC
    ATAAGTTCTGTTGGCTCTATACTAATACTAAAAGCCTTAGCACTTATTGGATATAACTCTAAATTAGATA
    GATCTATCATAATACCCCATGCTTCATCAGTTTTATACTCTTGTTTTTTATTAGAATTACCTAACTTAAT
    TTTCATATCTGTAGCACTTGCTTGTAATATACTCGAAACTTTCTCTTCAATTTTAGTTCTAACATTTGAT
    TTAATTAGAACACCAAGAGTAATACTTATTGTTGGATTTATAGAGCCTGGCGTTATAGATAAACGTATTA
    CACTAGCAGCAGCTGTAGCCGAATCAGCTTTCGTGACATCCTCTCCACCATCTATAGCAACGATATTTTT
    ATCAAGTTTCTCCCCAGCTATAGTTAAAGAATCAATAAATAATCTACAATTAGGATGAGATCCTATACAT
    GCAGTAGGATCAGTTCTCACATGAATGGTCTCGCCACTTGTTACCTGTTGTCTTGTTATCATCTCACTCA
    TAATCA
  • Protein Sequence : Show Sequence
    >gi|56708413|ref|YP_170309.1| intracellular growth locus, subunit C [Francisella tularensis subsp. tularensis SCHU S4]
    MIMSEMITRQQVTSGETIHVRTDPTACIGSHPNCRLFIDSLTIAGEKLDKNIVAIDGGEDVTKADSATAA
    ASVIRLSITPGSINPTISITLGVLIKSNVRTKIEEKVSSILQASATDMKIKLGNSNKKQEYKTDEAWGIM
    IDLSNLELYPISAKAFSISIEPTELMGVSKDGMRYHIISIDGLTTSQGSLPVCCAASTDKGVAKIGYIAA
    A
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice immunized with rLm/iglC were protected against lethal challenge with F. tularensis LVS administered by the intranasal route, a route chosen to mimic airborne infection, and, most importantly, against aerosol challenge with the highly virulent Type A F. tularensis SchuS4 strain (Jia et al., 2009).
  • Related Vaccine(s): F. tularensis vaccine rLm/iglC
14. IglC2
  • Gene Name : IglC2
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
  • VO ID : VO_0011073
  • NCBI Gene ID : 3191814
  • NCBI Protein GI : 56708721
  • Locus Tag : FTT_1712c
  • Genbank Accession : AJ749949
  • Protein Accession : YP_170617
  • Taxonomy ID : 177416
  • Gene Starting Position : 1791972
  • Gene Ending Position : 1792607
  • Gene Strand (Orientation) : -
  • Protein Name : intracellular growth locus, subunit C
  • Protein pI : 6.26
  • Protein Weight : 21340.81
  • Protein Length : 211
  • Protein Note : Similar to AAP58964.1 (Q7X3J5) IglC from Francisella novicida (23 kDa protein) (211 aa). BLAST Score = 405,Expect = e-112 Identities = 209/211 (99), Positives = 210/211 (99). Identical to FTT1357. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281
  • DNA Sequence : Show Sequence
    >gi|255961454:1791972-1792607 Francisella tularensis subsp. tularensis SCHU S4, complete genome
    ACTATGCAGCTGCAATATATCCTATTTTAGCAACTCCTTTATCTGTGCTAGCTGCGCAACATACTGGCAA
    ACTTCCTTGAGATGTTGTAAGACCATCTATAGATATAATATGATATCTCATTCCATCTTTTGAAACACCC
    ATAAGTTCTGTTGGCTCTATACTAATACTAAAAGCCTTAGCACTTATTGGATATAACTCTAAATTAGATA
    GATCTATCATAATACCCCATGCTTCATCAGTTTTATACTCTTGTTTTTTATTAGAATTACCTAACTTAAT
    TTTCATATCTGTAGCACTTGCTTGTAATATACTCGAAACTTTCTCTTCAATTTTAGTTCTAACATTTGAT
    TTAATTAGAACACCAAGAGTAATACTTATTGTTGGATTTATAGAGCCTGGCGTTATAGATAAACGTATTA
    CACTAGCAGCAGCTGTAGCCGAATCAGCTTTCGTGACATCCTCTCCACCATCTATAGCAACGATATTTTT
    ATCAAGTTTCTCCCCAGCTATAGTTAAAGAATCAATAAATAATCTACAATTAGGATGAGATCCTATACAT
    GCAGTAGGATCAGTTCTCACATGAATGGTCTCGCCACTTGTTACCTGTTGTCTTGTTATCATCTCACTCA
    TAATCA
  • Protein Sequence : Show Sequence
    >gi|56708721|ref|YP_170617.1| intracellular growth locus, subunit C [Francisella tularensis subsp. tularensis SCHU S4]
    MIMSEMITRQQVTSGETIHVRTDPTACIGSHPNCRLFIDSLTIAGEKLDKNIVAIDGGEDVTKADSATAA
    ASVIRLSITPGSINPTISITLGVLIKSNVRTKIEEKVSSILQASATDMKIKLGNSNKKQEYKTDEAWGIM
    IDLSNLELYPISAKAFSISIEPTELMGVSKDGMRYHIISIDGLTTSQGSLPVCCAASTDKGVAKIGYIAA
    A
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice immunized with rLm/iglC were protected against lethal challenge with F. tularensis LVS administered by the intranasal route, a route chosen to mimic airborne infection, and, most importantly, against aerosol challenge with the highly virulent Type A F. tularensis SchuS4 strain (Jia et al., 2009).
  • Related Vaccine(s): F. tularensis vaccine rLm/iglC
15. LpnA
  • Gene Name : LpnA
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
  • VO ID : VO_0011079
  • NCBI Gene ID : 3191792
  • NCBI Protein GI : 56708002
  • Locus Tag : FTT_0901
  • Genbank Accession : AJ749949
  • Protein Accession : YP_169898
  • Taxonomy ID : 177416
  • Gene Starting Position : 909953
  • Gene Ending Position : 910402
  • Gene Strand (Orientation) : ?
  • Protein Name : lipoprotein
  • Protein pI : 9.16
  • Protein Weight : 14909.46
  • Protein Length : 149
  • Protein Note : Paralog to conserved hypothetical lipoprotein lpnB.
  • DNA Sequence : Show Sequence
    >gi|255961454:909953-910402 Francisella tularensis subsp. tularensis SCHU S4, complete genome
    TATGAAAAAAATAATTGAGCTTAGTCTTTTATCTTTATCAATCGCAGGTTTAGCGAGCTGTTCTACTCTA
    GGGTTAGGTGGCTCTGATGATGCAAAAGCTTCAGCTAAAGATACTGCTGCTGCTCAGACAGCTACTACTG
    AGCAAGCTGCTGCTGTATCTAAGCCAACTGCAAAAGTAAGTTTAAATAAACTTGGTCAGGATAAAATAAA
    AGCAACTGTATATACAACATACAATAATAACCCACAAGGAAGTGTAAGATTACAATGGCAGGCTCCAGAA
    GGTTCTAAGTGCCATGATACAAGCTTCCCAATTACTAAGTATGCTGAGAAGAACGATAAAACTTGGGCAA
    CTGTAACAGTTAAGCAAGGTAATAACTTCTGTAGCGGTAAGTGGACAGCTAATGTAGTTTATGACAAAGA
    AGTAATCGCTTCTGATTCAATAAATATTTA
  • Protein Sequence : Show Sequence
    >gi|56708002|ref|YP_169898.1| lipoprotein [Francisella tularensis subsp. tularensis SCHU S4]
    MKKIIELSLLSLSIAGLASCSTLGLGGSDDAKASAKDTAAAQTATTEQAAAVSKPTAKVSLNKLGQDKIK
    ATVYTTYNNNPQGSVRLQWQAPEGSKCHDTSFPITKYAEKNDKTWATVTVKQGNNFCSGKWTANVVYDKE
    VIASDSINI
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Anti-lpnA MAbs conferred significant, although not complete, protection of naïve C3H/HeN mice from lethal challenge with F. tularensis LVS when administered therapeutically (Savitt et al., 2009).
16. LPS
  • Gene Name : LPS
  • Gene Strand (Orientation) : ?
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice immunized intranasally with J5dLPS/OMP exhibited protective immunity to intratracheal challenge with the live vaccine strain, as well as the highly-virulent SchuS4 strain, of F. tularensis. J5dLPS/OMP is a novel vaccine construct consisting of detoxified, O-polysaccharide side chain-deficient, lipopolysaccharide non-covalently complexed with the outer membrane protein of N. meningitidis group B. (Gregory et al., 2010).
  • Additional Molecule Role : Protective antigen
17. PurC/PurD
  • Gene Name : PurC/PurD
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica LVS
  • NCBI Gene ID : 3952576
  • NCBI Protein GI : 89255807
  • Locus Tag : FTL_0396
  • Genbank Accession : AM233362
  • Protein Accession : YP_513169
  • Taxonomy ID : 376619
  • Gene Starting Position : 365666
  • Gene Ending Position : 367978
  • Gene Strand (Orientation) : ?
  • Protein Name : fusion protein PurC/PurD
  • Protein pI : 5.81
  • Protein Weight : 82357.33
  • Protein Length : 770
  • Protein Note : 6.3.2.6
  • DNA Sequence : Show Sequence
    >gi|89255449:365666-367978 Francisella tularensis subsp. holarctica LVS chromosome, complete genome
    AATGTTTGATAAACAAATCATTGCTAATAATATAAAAAATGTTCTAAAATCAACAAATCTTGATATCAAG
    AATAAATATATAGGTAAAGTAAGAGATATGTACTTTACTGATGACAAAAGTATTTTGATATCTACAGATA
    GACAATCTGCTTTTGATAGATCTTTGGGTTTTATACCATTTAAGGGGCAAATCCTTGCACAATCATCAGT
    ATGGTGGTTCAAAGAAACAGCACATATTGTCAAAAACCACTTTATAGACTCTCCAGATCCAAATGTTGTC
    ATCGCGAGAAAGGCAAAAGTATTGCCAATTGAGTTTGTCGTTAGAGGTTATATAACTGGTTCAACTTCTA
    CATCATTGTGGACACATTATAAAAACGGTAGCCGTGACTATTGTGGCAATATCCTGCCAGAAGGTTTAAA
    AAAGAATCAAAAATTACCACAAAATATCCTTACACCAACTACCAAAGAGCAAGATCATGATCGCCCAATT
    TCAGCTGAGGATATTGTCAAGGAAGGTTGGCTAACTCAGCAGCAGTGGGATTTTGCTTCACAAAAGGCTC
    TAGAACTTTTTGAGTTTGGACAAAAAAAAGCCTTAGAACATGGCTTATTTTTGGCGGATACTAAATATGA
    GTTTGGTATTGATGAGCAAACTGGAGAGATTATTTTGATCGATGAGATCCATACTCCAGATAGCTCAAGA
    TTTTGGCTAAAAGATAGCTATGCTACAAGATTTGAAAATGGTGAAGAACCAGAGAATATTGATAAAGAAT
    TTTTTAGATTATGGTTTGCAAAAAATTGTGACCCATATAATGATGAAGTTTTACCACAAGCACCACAAGA
    GTTAGTTGTCGAATTATCTCAAAAATATATAACCCTTTTTGAAATGATAACAGGGCAAAAGTTTGAAGTA
    CCAAGAGATTTAGAAAATATAAATCAACGTATTGTTAAAAATGTTACAGATTACTTAAATATGGAGAAGC
    CAGTGAATATTCTTCTAGTAGGATCAGGAAGTAGAGAGCATGCGATAGCAGAAGCAGTCAAAAGAAGCTC
    TATTGCAAATAAGCTTTTTTGTATAAGCACAGCGATTAACCCTGCGATAGATAAGATAACGCAAGGGTAT
    CAAATCGCTGATATTTGTAATTGTGATGAAGTACTAGAGTATGCAAAATCACAAAGTATTGATATTGCGA
    TTATCGGTCCTGAAGCACCACTTGAGGCTGGTCTAGCTGATGCACTTAAGACAGCAGCAATTGGCGTAGT
    TGGTCCAACCAAAAAGTTAGCACAGCTTGAAACATCAAAAGGTTTCACTAGAGATCTGATCCGTGATTAT
    GATATAGGCGCTAATCCTTTCTTTAGAAAGTTTAACTCGATGGATGGAGTCGAAGAGACTATCAAAAAAT
    ATCAAAATCAATTTGTCATAAAAGCTGATGGTTTATGTGGTGGTAAAGGAGTGCTTGTTTGGGGCGATCA
    TTTGCACAGCCTTGATGAGGCGATTAGACACTGTCAGTCATTGGTAGATGCTGGCAAAGAGTTTGTTATC
    GAGGAGAAGCTTGTTGGCCAGGAGTTCTCGCTGATATCATTTACTGATGGCAAAAATTTTATCCATATGC
    CAGCAGTCCAAGATCATAAGCGTGCCCATGAAGGTGACAAAGGTCCTAATACCGGTGGTATGGGTACTTA
    TTCTGATGCTAATCATAGTTTACCGTTTTTATCAGCTGCTGATATCGAAAGAGCAAAACAAATAAATGAG
    AAAGTTGTCAGAGCTTTAGCTGATAAATTTTGTGAACCATATCAAGGTATCCTTTATGGTGGCTTTATGG
    CGACAAAAGATGATACTAAAGTGATCGAGTATAATGCACGTTTTGGTGATCCAGAAGCGATGAATCTACT
    TACACTGTTAGAGACAGATTTTGTTGAGATTGCTCAAGCTATTACACAGGGTAAACTTGATACGGTCAAA
    GCTAAGTTTAAAAATCAAGCAAGCGTATGTAAATATCTAGTACCGCTTGGCTATCCAAACCAATCAGTTA
    AAAACTTTGAGATAGATATTTCACAATGCCCTGATAATGTCGAGCTTTTCTTAGGTGCTGTTGATTATAA
    AGATGGTAAGCTAATTGGTACAGGTTCTAGAGCTATAGCTGTACTTGGTTTAGGTGATACAATAGCTGAA
    GCAGAGCAAAAAGCCGAAAATGCTGTCAAAAATATTTATGGTAAACTATTTCATCGTCCAGATATTGGTA
    CTAAAGAGCTTATCAATAAGCGTATCAAACACATGAATTTGTTGCGTGGTGATAAATATCAGGAGCTTAA
    ATA
  • Protein Sequence : Show Sequence
    >gi|89255807|ref|YP_513169.1| fusion protein PurC/PurD [Francisella tularensis subsp. holarctica LVS]
    MFDKQIIANNIKNVLKSTNLDIKNKYIGKVRDMYFTDDKSILISTDRQSAFDRSLGFIPFKGQILAQSSV
    WWFKETAHIVKNHFIDSPDPNVVIARKAKVLPIEFVVRGYITGSTSTSLWTHYKNGSRDYCGNILPEGLK
    KNQKLPQNILTPTTKEQDHDRPISAEDIVKEGWLTQQQWDFASQKALELFEFGQKKALEHGLFLADTKYE
    FGIDEQTGEIILIDEIHTPDSSRFWLKDSYATRFENGEEPENIDKEFFRLWFAKNCDPYNDEVLPQAPQE
    LVVELSQKYITLFEMITGQKFEVPRDLENINQRIVKNVTDYLNMEKPVNILLVGSGSREHAIAEAVKRSS
    IANKLFCISTAINPAIDKITQGYQIADICNCDEVLEYAKSQSIDIAIIGPEAPLEAGLADALKTAAIGVV
    GPTKKLAQLETSKGFTRDLIRDYDIGANPFFRKFNSMDGVEETIKKYQNQFVIKADGLCGGKGVLVWGDH
    LHSLDEAIRHCQSLVDAGKEFVIEEKLVGQEFSLISFTDGKNFIHMPAVQDHKRAHEGDKGPNTGGMGTY
    SDANHSLPFLSAADIERAKQINEKVVRALADKFCEPYQGILYGGFMATKDDTKVIEYNARFGDPEAMNLL
    TLLETDFVEIAQAITQGKLDTVKAKFKNQASVCKYLVPLGYPNQSVKNFEIDISQCPDNVELFLGAVDYK
    DGKLIGTGSRAIAVLGLGDTIAEAEQKAENAVKNIYGKLFHRPDIGTKELINKRIKHMNLLRGDKYQELK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A purMCD mutant in Francisela tularensis was attenuated. A low dosage of F. tularensis LVS delta purMCD mutant provided protection against challenge in mice (Pechous et al., 2006).
  • Related Vaccine(s): Francisella tularensis purMCD mutant vaccine
18. purF
  • Gene Name : purF
  • Sequence Strain (Species/Organism) : Francisella novicida U112
  • NCBI Gene ID : 4548578
  • NCBI Protein GI : 118498261
  • Locus Tag : FTN_1700
  • Genbank Accession : CP000439
  • Protein Accession : YP_899311
  • Taxonomy ID : 401614
  • Gene Starting Position : 1820533
  • Gene Ending Position : 1822023
  • Gene Strand (Orientation) : -
  • Protein Name : amidophosphoribosyltransferase
  • Protein pI : 7.32
  • Protein Weight : 51173.39
  • Protein Length : 496
  • Protein Note : Catalyzes first step of the de novo purine nucleotide biosynthetic pathway
  • DNA Sequence : Show Sequence
    >gi|118496615:1820533-1822023 Francisella tularensis subsp. novicida U112, complete genome
    ATTAGTTATCAAACCCTTTGTATTTTTTTTCTAATTCTTTTAACTCTTTACGATGTTTCTCTAGCGCATC
    TAGATAAGCATCATCGACATCACCTGTAATGTAATTACCAGAGAAAACACTATCCTCAAACTCTCTAATT
    TTAGGGTTTTGCTTTTGAATAATCTCTTTTAGATCCTCTAATGGTAAATATATTAGTCCATCTACTCCAA
    TCCACTGGCGAATCTCTTCTATAGTCTTTCCATGAGCAATTAAATCCGACTTAACCGGCATATCAATACC
    ATAAACATTTGGATAACGAACGGCTGGAGATACTGAAGCAAGATAAACAGATTTAGCACCTAAATCTCTA
    ATCATCTCAATAATCCGCTTAGATGTTGTACCTCGGACAATAGAATCATCAACAAGTAGAACATTCTTAT
    CTCTAAACTCTGCTGGAATAGGGTTTAGTTTTCTTCTAACAAAATTCTTTCTATCAACATTCTCTGGCAT
    GATAAAAGTTCTACCAACATAGCGATTCTTAACAAAACCTTCACGATACTCAACACCTAATGCTGTTGCA
    ATCTCTTGAGCAGAAGCTCTACCTGTCTCAGGTACAGGGATAACGATATCGATATCCTTATCTTTCCACG
    CTTCTTTGATTCTCTTACTTAGAATTTTACCAGCATCTACTCGCGCTTGATAAACACTCACACCATTCAT
    AATACTATCAGGACGTGCAAAGTATACATACTCAAATAGACACGGTGCCAAAACTGGATTTTTTGCACAG
    ATTTTAGAGTGAACCTTTCTATCCTCTGTAATTATTATCACCTCACCAGGTTCAACATCACGTAATACTT
    TAAATCCGGAAATATCTAAAGCAACACTTTCACTAGCAACCATGTAAGCTTTTTCACCATCATCGTACTC
    TTTAAAACCAAGCACAAGTGGACGAATACCATAAGGATCTCTAAAAGCAATTAAACCAAAATTTGCTATC
    ATCGCAGTACAAGCATAGCCCCCTTTGACATGTTCAAAAACAAATTTACATGCTTTATAAACAGCCTCAG
    AAGTCGCAGATCCTTTTGATTTATTCATCCCACAAGCAAAGAAATTAAGCAATAATTCGGAATCTGAACT
    TGTATTAAGATGGCGTCTTTCAATATCATGGAGCATTTGTGCAAGCTCTGGCACATTAGTGAGATTGCCA
    TTATGAGCAAAGACTATACCATGAGGATTATTTACATAAAAAGGTTGGCTATCAGCAGCACCCAAGCTAC
    CTGCTGTAGGATATCTAACATGACCGATACCCATATTACCTTTTGACTTCTCTAGTTTTTCATCTGTAAA
    TACATCACTTACTAGACCTGTATTTTTACGGATAAAAAAGTGCCCACGATCCATAGTTGCAATCCCTGCA
    GCATCTTGACCTCTATGCTGCAAAAGACTTAAACCATAAAATAATGCATAACTAACCTGATCTGGTCCAG
    CGACTCCAATTACTCCACACA
  • Protein Sequence : Show Sequence
    >gi|118498261|ref|YP_899311.1| amidophosphoribosyltransferase [Francisella tularensis subsp. novicida U112]
    MCGVIGVAGPDQVSYALFYGLSLLQHRGQDAAGIATMDRGHFFIRKNTGLVSDVFTDEKLEKSKGNMGIG
    HVRYPTAGSLGAADSQPFYVNNPHGIVFAHNGNLTNVPELAQMLHDIERRHLNTSSDSELLLNFFACGMN
    KSKGSATSEAVYKACKFVFEHVKGGYACTAMIANFGLIAFRDPYGIRPLVLGFKEYDDGEKAYMVASESV
    ALDISGFKVLRDVEPGEVIIITEDRKVHSKICAKNPVLAPCLFEYVYFARPDSIMNGVSVYQARVDAGKI
    LSKRIKEAWKDKDIDIVIPVPETGRASAQEIATALGVEYREGFVKNRYVGRTFIMPENVDRKNFVRRKLN
    PIPAEFRDKNVLLVDDSIVRGTTSKRIIEMIRDLGAKSVYLASVSPAVRYPNVYGIDMPVKSDLIAHGKT
    IEEIRQWIGVDGLIYLPLEDLKEIIQKQNPKIREFEDSVFSGNYITGDVDDAYLDALEKHRKELKELEKK
    YKGFDN
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Mice, which had been immunized with at least 6.95×103 cfu of F. tularensis U112 purF::Cm, were protected against
    the subsequent F. tularensis subspecies novicida challenge (Quarry et al., 2007).
  • Related Vaccine(s): Francisella tularensis purF mutant vaccine
19. purM
  • Gene Name : purM
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica LVS
  • NCBI Gene ID : 3952575
  • NCBI Protein GI : 89255806
  • Locus Tag : FTL_0395
  • Genbank Accession : AM233362
  • Protein Accession : YP_513168
  • Taxonomy ID : 376619
  • Gene Starting Position : 364623
  • Gene Ending Position : 365666
  • Gene Strand (Orientation) : ?
  • Protein Name : phosphoribosylaminoimidazole synthetase
  • Protein pI : 5.23
  • Protein Weight : 35240.61
  • Protein Length : 347
  • Protein Note : catalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis
  • DNA Sequence : Show Sequence
    >gi|89255449:364623-365666 Francisella tularensis subsp. holarctica LVS chromosome, complete genome
    AATGGCAGGCTTAAAATATGAAGATGCTGGCGTAAATATCGAAGCTGGTAATCAAGCTGTAGAAAGAATG
    AAGCAGCATGTCAAAAAAACTTTTACCCAAGATGTTTTGACAGGTTTGGGTAGCTTTGGTTCTTTATACT
    CACTAAAAAATATTATAAATAATTATGATGATCCTGTCTTAGTACAGTCGATTGATGGTGTTGGTACAAA
    GACAAAAGTCGCGGTAATGTGTGGTAAGTTTGAAAACCTTGGTTATGATCTTTTCTCAGCTGCGACAAAT
    GATATTGTTGTAATGGGTGCCAAGCCAATTACGTTTCTAGATTATGTCGCGCATGATAAGCTTGATCCTG
    CAATTATGGAAGAGCTCGTCAAAGGGATGTCAAAAGCTTGTGCTGAGTGTGGGGTTTCGCTGGTAGGTGG
    CGAGACTGCGGAAATGCCTGGAGTATATCAAGCTGGTGAGATTGACATGGTTGGAGTTATCACTGGTATC
    GTTGGTAGAAAAAGAATTATCAATGGTGAGAATATCAAAGAGGGTGATATTGTCTTTGGGCTTAGTTCAT
    CAGGATTACATACAAATGGCTACTCTTTTGCACGTAAACTATTTTTTGATGTAGCAGGCAACAAACATAC
    TGATACTTATCCAGAGTTAGAGGGTAAAACTATCGGTGATGTGCTACTTGAACCACATATCAACTATACT
    AATATCATCCATGATTTCTTAGATAATGGTGTTGATATCAAAGGTATGGCACATATCACAGGTGGCGGGT
    TTATCGAGAATATTCCACGCGTATTACCACAAGGTTTAGGTGCCCAAATTGACAAAGATAGTTTTGCAAC
    TCCAGCTATTTTTAAGCTAATGCAGAGAATCGGCGATATAAGCGAGTTTGAAATGTATCGCTCATTTAAT
    ATGGGTATTGGTATGACGATAATTGCTAGCCAAGATCAATTTGACAAGATGCAAGAATTAGCTAAAAAAC
    ATACTAATACAAAACTATATCAAATTGGTAAAATCACAAATTCAGGAAAGGTAGAAATTATCTA
  • Protein Sequence : Show Sequence
    >gi|89255806|ref|YP_513168.1| phosphoribosylaminoimidazole synthetase [Francisella tularensis subsp. holarctica LVS]
    MAGLKYEDAGVNIEAGNQAVERMKQHVKKTFTQDVLTGLGSFGSLYSLKNIINNYDDPVLVQSIDGVGTK
    TKVAVMCGKFENLGYDLFSAATNDIVVMGAKPITFLDYVAHDKLDPAIMEELVKGMSKACAECGVSLVGG
    ETAEMPGVYQAGEIDMVGVITGIVGRKRIINGENIKEGDIVFGLSSSGLHTNGYSFARKLFFDVAGNKHT
    DTYPELEGKTIGDVLLEPHINYTNIIHDFLDNGVDIKGMAHITGGGFIENIPRVLPQGLGAQIDKDSFAT
    PAIFKLMQRIGDISEFEMYRSFNMGIGMTIIASQDQFDKMQELAKKHTNTKLYQIGKITNSGKVEII
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A purMCD mutant in Francisella tularensis was attenuated. A low dose of the F. tularensis LVS delta purMCD mutant provided protection against subsequent lethal challenge with the LVS parental strain (Pechous et al., 2006).
  • Related Vaccine(s): Francisella tularensis purMCD mutant vaccine
20. SodB from F. tularensis SCHU S4
  • Gene Name : SodB from F. tularensis SCHU S4
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
  • VO ID : VO_0011077
  • NCBI Gene ID : 3190713
  • NCBI Protein GI : 56707247
  • Locus Tag : FTT_0068
  • Genbank Accession : AJ749949
  • Protein Accession : YP_169143
  • 3D structure: PDB ID : 3H1S
  • Taxonomy ID : 177416
  • Gene Starting Position : 70160
  • Gene Ending Position : 70738
  • Gene Strand (Orientation) : ?
  • Protein Name : superoxide dismutase (Fe)
  • Protein pI : 5.29
  • Protein Weight : 20748.47
  • Protein Length : 192
  • Protein Note : Similar to SODF_ECOLI (P09157) Superoxide dismutase [Fe] from E. coli (192 aa). FASTA: opt: 896 Z-score: 1143.2 E(): 8.8e-56 Smith-Waterman score: 896; 66.316 identity in 190 aa overlap
  • DNA Sequence : Show Sequence
    >gi|255961454:70160-70738 Francisella tularensis subsp. tularensis SCHU S4, complete genome
    AATGAAATTTGAATTACCAAAACTACCTTACGCTGTTGATGCATTAGAGTCAACAATATCAAAAGAAACA
    ATAGAGTATCACTATGGTAAACATCATCAAACATATGTAACTAATCTAAATAATTTAGTTGAGGGTACAG
    AGCACGATGGCAGAAACCTAGAAGAAATCGTAAAAACTTCTAATGGCGGAATATTTAATAACGCTGCTCA
    AGTTTTTAATCATACTTTTTACTGGAATTGTTTAACTCCAAACAAAACAGAAGCTTCAAGTCAGTTAAAA
    GCAGCATTGATCGAGACATTTGGTTCTGTAGAAAATTTTAAAGAACAATTCTCTAAGGCAGCTATTGCAA
    CATTTGGTTCTGGTTGGGCTTGGTTAGTAAAAAATACTGAAGGTAAACTTGAAATAGTAACTACAAGTAA
    CGCTGGTTGCCCATTAACAGAGAACAAAAAGCCATTGCTAACTTTTGATGTTTGGGAGCACGCATACTAT
    ATTGATTATCGTAATGCTAGACCTAAATATGTTGAAGCATTATGGGATATCGTAAACTGGCAATTTGTTT
    CTGAGCAATTCGCTGATTA
  • Protein Sequence : Show Sequence
    >gi|56707247|ref|YP_169143.1| superoxide dismutase (Fe) [Francisella tularensis subsp. tularensis SCHU S4]
    MKFELPKLPYAVDALESTISKETIEYHYGKHHQTYVTNLNNLVEGTEHDGRNLEEIVKTSNGGIFNNAAQ
    VFNHTFYWNCLTPNKTEASSQLKAALIETFGSVENFKEQFSKAAIATFGSGWAWLVKNTEGKLEIVTTSN
    AGCPLTENKKPLLTFDVWEHAYYIDYRNARPKYVEALWDIVNWQFVSEQFAD
  • Molecule Role : Virmugen
  • Molecule Role Annotation : C57BL/6 mice were vaccinated intranasally (i.n.) with the sodB(Ft) mutant and challenged i.n. with lethal doses of F. tularensis SchuS4. SodB(Ft) vaccinated mice following SchuS4 challenge exhibited significantly reduced bacterial burden in lungs, liver, and spleen, regulated production of pro-inflammatory cytokines, and less severe histopathological lesions compared to the LVS vaccinated mice. The sodB(Ft) vaccination induced a potent humoral immune response and protection against SchuS4 required both CD4 and CD8 T cells in the vaccinated mice (Bakshi et al., 2008).
  • Related Vaccine(s): sodB(Ft)
21. sodC
  • Gene Name : sodC
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica FTNF002-00
  • NCBI Gene ID : 5522867
  • NCBI Protein GI : 156501771
  • Locus Tag : FTA_0403
  • Genbank Accession : CP000803
  • Protein Accession : YP_001427836
  • Taxonomy ID : 458234
  • Gene Starting Position : 350894
  • Gene Ending Position : 351451
  • Gene Strand (Orientation) : +
  • Protein Name : copper/zinc superoxide dismutase
  • Protein pI : 5.61
  • Protein Weight : 18894.63
  • Protein Length : 185
  • Protein Note : 1.15.1.1
  • DNA Sequence : Show Sequence
    >gi|156501369:350894-351451 Francisella tularensis subsp. holarctica FTNF002-00 chromosome, complete genome
    AATGACTGCTTTTTATAAATTATGTGGAATGAGTATGCTTTCACTTGTATTAGCAGACTGCACATTCTTG
    TCAGCAAATAAGCCTTATGATCGCGACCATGATGGTGAGTTAATTGTACATATGAAAGATGTCAATACTC
    ATAAAGAGGTTGGAACAATCACAATATCACCATATATTCATGATGGTAACCAAGAAGGGATGTTGATTAC
    GCCACATTTATATAATTTACCTGCTAATACTACTCATGGCATGCATATCCATATCAATCCTAGTTGTGAA
    GATAATGGTATCGCAGCAGGTGGGCATTGGGATCCAGATAATACGCAAAAGCACCTTGGCCCATACAATG
    ATAATGGACATAAGGGCGATTTGCCTGTACTAGTTGTTAACGCAGATGGTACAGCAACTGAGCCTGTTGT
    AGCGCCAAAGCTTAATTCTCTAGAAGAGCTAGCGGGTCATAGTTTGATGCTTCATGCGGGAGGCGATAAT
    TACTCTGATAAGCCACAACCTCTAGGCGGAGGTGGTGCTAGAATGTGGTGTGGAGTTATAGCAGACTA
  • Protein Sequence : Show Sequence
    >gi|156501771|ref|YP_001427836.1| copper/zinc superoxide dismutase [Francisella tularensis subsp. holarctica FTNF002-00]
    MTAFYKLCGMSMLSLVLADCTFLSANKPYDRDHDGELIVHMKDVNTHKEVGTITISPYIHDGNQEGMLIT
    PHLYNLPANTTHGMHIHINPSCEDNGIAAGGHWDPDNTQKHLGPYNDNGHKGDLPVLVVNADGTATEPVV
    APKLNSLEELAGHSLMLHAGGDNYSDKPQPLGGGGARMWCGVIAD
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A sodC mutant in Francisella tularensis subspecies holarctica was attenuated and provided protection and cleared the infection against lethal challenge in nearly 50% of the mice infected with the ΔsodC mutant (Melillo et al., 2009).
  • Related Vaccine(s): Francisella tularensis sodC mutant vaccine
22. TUL4
  • Gene Name : TUL4
  • Sequence Strain (Species/Organism) : Francisella tularensis
  • VO ID : VO_0011076
  • NCBI Protein GI : 148688
  • Taxonomy ID : 263
  • Gene Strand (Orientation) : ?
  • Protein Name : 17 kDa major membrane protein (TUL4) precursor
  • Protein Length : 149
  • Protein Sequence : Show Sequence
    >gi|148688|gb|AAA24919.1| 17 kDa major membrane protein (TUL4) precursor [Francisella tularensis]
    MKKIIKLSLLSLSIAGLASCSTLGLGGSDDAKASAKDTAAAQTATTEQAAAVSKPTAKVSLNKLGQDKIK
    ATVYTAYNNNPQGSVRLQWQAPEGSKCHDTSFPITKYAEKNDKTWATVTVKQGNNFCSGKWTANVVYDKE
    VIASDSINI
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : A 17-kDa lipoprotein, TUL4, of the facultative intracellular bacterium Francisella tularensis is one of several membrane proteins that induce an in vitro response in T cells from F. tularensis-primed humans. When mice were immunized with S. typhimurium chi 4072(pTUL4-15), some animals showed an antibody response and a T-cell response to TUL4. The present study demonstrated that the 17-kDa lipoprotein TUL4 of F. tularensis is involved in a protective immunity to tularemia (Sjöstedt et al., 1992).
  • Related Vaccine(s): F. tularensis vaccine X4072(pTUIA-15)
23. wbtA
  • Gene Name : wbtA
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. tularensis SCHU S4
  • NCBI Gene ID : 3191939
  • NCBI Protein GI : 56708505
  • Locus Tag : FTT_1464c
  • Genbank Accession : AJ749949
  • Protein Accession : YP_170401
  • Taxonomy ID : 177416
  • Gene Starting Position : 1514679
  • Gene Ending Position : 1516415
  • Gene Strand (Orientation) : -
  • Protein Name : dTDP-glucose 4,6-dehydratase
  • Protein pI : 8.34
  • Protein Weight : 60861.77
  • Protein Length : 578
  • Protein Note : Similar to Q9S4V4 PglF from Campylobacter jejuni (590 aa). FASTA: opt: 1343 Z-score: 1435.9 E(): 3.9e-72 Smith-Waterman score: 1378; 43.543identity in 604 aa overlap
  • DNA Sequence : Show Sequence
    >gi|255961454:1514679-1516415 Francisella tularensis subsp. tularensis SCHU S4, complete genome
    ACTACCCATTCAATCTATGTTCAAATTCCGGAACAATTTTCTTTAATATCACAAGCTGATCAACATCATC
    CTTGATCAACGATTCAATATCTTGGTTTAGAGTATTAATATCGTAAAAAGTCCTTCTACCAATAAAAATA
    TCTTTATAGTCGGTACTAACATCATCTTCCTCTATCAAAAGCTCTTCGTAAAGTTTCTCTCCTGGACGCA
    AACCAACTATTTTAATATCAATATCACCTCTACCAGAAAGTCTAATAAATTGTTTAGCAAGATCAATAAT
    CTTGACAGGTTGCCCCATATCTAAGACAAAGACCTCTGAATTTTTTGCAATAGCACCAGCTTGTAGGACC
    AGTTCACAAGCTTCTGGTATCAACATAAAATAACGTGTAATTTCAGGATGAGTAACTGTAACAGGACCAC
    CTTTTCTTATTTGCTCTTCAAATTTTGGAATCACACTGCCACTACTACCAAGCACATTACCAAAACGCAC
    TGCAGCAAGCTTGGTATTTTTGGGATCAACATTCTGTAAATACAGCTCACAAACTCTCTTGGTAGCCCCC
    ATAACATTCGTTGGTCGCACTGCTTTATCAGTGGAAATCAATATAAATGACTCAACACCAGCTTCTATAG
    CCAGATCTATAGCATTCTTAGTACCTAAGATATTATTTCTAATTGCTCTAGAGATATTCTCCTCAACTAA
    GGGAACATGCTTGTAGGCAGCAGCATGAAATACTATATTTGGAGTATACTTTTGAAAAACCTCAGCCAAT
    GCTTTTCTATCACAAACAGAACATAGCACACTATTGATATTAAAATGACTACACTCCTCAGTAATTTTAT
    ATAAGTTAAACTCACTATGATCAACCAATATCAACTCTTTTGCCTGATACTTGATACATTGATGTACTAT
    TTCAGAACCTATACTACCTCCAGCTCCTGTGACTAGCACCACCTTATTTTTGATAAAATTAGAGATAGAT
    TCTTTATCTAAACTCTTAGTATCACGCGCTAATAGATCATAGAGTGAAACAGGCTTCAACTGTGACATAA
    AATTCTCATCTTGAAGAATTTCCTCAAGAGGCGGCATAATTCTAATCTGATTAAAATCCTTTTCAAATTC
    TTTATATATATTTTTGACTACTTGGTTTGCATTTCTTGGCAATGCAATAACCAAAAGGTCAAATCTACTA
    GATAGCAATAGTTTTGTTAATTCAGCTTTAGATAGAACCTTTTTACTATCAATACTTCTTTTTTGTAAAG
    TTTCATTGTCATCAACAAAACATTTGATGCGATAACCAGCAGAAGCAAGTTCTTGAGCAATCTTTGTTCC
    TGCAGCACCTGCACCATAAATAACCGCAGTTTTACTTTTATCCACTGATCCTCTGTTCATCAAATACCAA
    TAAAAGTAGACACTTAAACTGATCAAAAAAACATAAAATAAAAACTCAGAGAATATCAACGAAAAAGTGA
    CTTTGCCATAAAAAAATAATGCCACTATGAAAAATACTGGCAAATTAATAAAAGCCTTACGTAAAAAAGT
    TTTTTGAGTCGACTTACGCCAACTAGCCATATAATCTCTAAGTAGTAAAAAAGATGACAAGCATCTCAGC
    AAAACTAATGCAAGTAAAAAATGTAAATTAACATCTTGCTTGAAAATATAGAAAGTCCAATTAACAGTAA
    TAATAGTTAAAACTATTATTACCACGAAATTAAGCGTTCTATTATCGTAGAAAGACA
  • Protein Sequence : Show Sequence
    >gi|56708505|ref|YP_170401.1| dTDP-glucose 4,6-dehydratase [Francisella tularensis subsp. tularensis SCHU S4]
    MSFYDNRTLNFVVIIVLTIITVNWTFYIFKQDVNLHFLLALVLLRCLSSFLLLRDYMASWRKSTQKTFLR
    KAFINLPVFFIVALFFYGKVTFSLIFSEFLFYVFLISLSVYFYWYLMNRGSVDKSKTAVIYGAGAAGTKI
    AQELASAGYRIKCFVDDNETLQKRSIDSKKVLSKAELTKLLLSSRFDLLVIALPRNANQVVKNIYKEFEK
    DFNQIRIMPPLEEILQDENFMSQLKPVSLYDLLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQ
    CIKYQAKELILVDHSEFNLYKITEECSHFNINSVLCSVCDRKALAEVFQKYTPNIVFHAAAYKHVPLVEE
    NISRAIRNNILGTKNAIDLAIEAGVESFILISTDKAVRPTNVMGATKRVCELYLQNVDPKNTKLAAVRFG
    NVLGSSGSVIPKFEEQIRKGGPVTVTHPEITRYFMLIPEACELVLQAGAIAKNSEVFVLDMGQPVKIIDL
    AKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDDVSTDYKDIFIGRRTFYDINTLNQDIESLIKDDVD
    QLVILKKIVPEFEHRLNG
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A wbtA mutant in Francisella tularensis was attenuated and provided protection in mice against challenge with an otherwise lethal dose of either F. tularensis LVS or a fully virulent clinical isolate of F. tularensis type B (Sebastian et al., 2007).
  • Related Vaccine(s): Francisella tularensis wbtA mutant vaccine
24. WbtI
  • Gene Name : WbtI
  • Sequence Strain (Species/Organism) : Francisella tularensis subsp. holarctica FTNF002-00
  • VO ID : VO_0011074
  • NCBI Gene ID : 5522688
  • NCBI Protein GI : 156502002
  • Locus Tag : FTA_0635
  • Genbank Accession : CP000803
  • Protein Accession : YP_001428067
  • Taxonomy ID : 458234
  • Gene Starting Position : 590832
  • Gene Ending Position : 591914
  • Gene Strand (Orientation) : +
  • Protein Name : DegT/DnrJ/EryC1/StrS family sugar aminotransferase
  • Protein pI : 6.8
  • Protein Weight : 37905.36
  • Protein Length : 360
  • Protein Note : component of putative O antigen gene complex
  • DNA Sequence : Show Sequence
    >gi|156501369:590832-591914 Francisella tularensis subsp. holarctica FTNF002-00, complete genome
    AATGAGTAAAGTAAATGTAACAAAACCATATTTACCAGATATAAATAAATATAAAAGCTATGTAAATAAA
    ATATACAAAAATGGATGGCTTACTAATAATGGTCCGTTAGTGCAAGAGCTAGAAAAAAGACTTGCAAAGT
    ATCTAGGTGTTAAAAATATAGTTTTAGTATCAAATGGTACAATTGCATTAGAAATCGCGTATAGAGCGTT
    AGGAGTCAAAGGAAGTGCAATTACTACTCCATTTTCATTTGTCGCTACTACATCTTCATTGGTTTCTAAC
    AATGTAAAACCAGTGTTTGTTGATATTGATGAGAATACTCTAAGTATAGACGTCTCTAAAATTAAGTATG
    CTATTGAAGAGGATACTTCAGCTATTGTGCCAGTTCATGTGTTTGGAAATGGTTGTGAAGTTGAAAAAAT
    AGACATGCTGGCTAAAAAACATAACTTAAAAGTTATTTATGATGCAGCACATGCTTTTGATGTTAAGTAT
    AAGGGTGAGAGTATATTAAACTATGGTGATATTTCGACATTAAGTTTTCATGCAACAAAGATTTTTCATT
    CTATTGAAGGAGGTGCGCTTATCATTAATGATGATAGTCTTGTTGAAAAAGTTCGTTATTTCATTAATTT
    TGGTATAGAAAGCTCAGAATCAATACCTTACTTAGGTACTAATGCTAAAATGAATGAATTTGAGGCGGCT
    ATGGGACTTTGTGTTCTAGATGATATTATAGAAATTAAGAGCAAAAGGAAAGTTATTACAGAGATATATG
    AGGCTGGGTTAGATGGATTGGTAAAGTTTCAAGAACAGAATCAGCATTCTAGTAGGAATTATAGCTATTT
    TCCAGTAATATTTAGGACTGAGGAGGAACTTCTCAGAGTACAGAAAGCACTAATACAAAATGATATAATA
    TCGCGTAGATATTTTTATCCATCATTAGATAGTCTTAGTTATATAGAGCCAAAGCAGTATATGCCAATCT
    CAAGAGATATATCTAAAAGAATATTATGTTTGCCAATTTATGCAGAGTTAGAAGACGATAAAATTAATAA
    AATAATTAATAATATCAAAGAGGTTTCCTCATG
  • Protein Sequence : Show Sequence
    >gi|156502002|ref|YP_001428067.1| DegT/DnrJ/EryC1/StrS family sugar aminotransferase [Francisella tularensis subsp. holarctica FTNF002-00]
    MSKVNVTKPYLPDINKYKSYVNKIYKNGWLTNNGPLVQELEKRLAKYLGVKNIVLVSNGTIALEIAYRAL
    GVKGSAITTPFSFVATTSSLVSNNVKPVFVDIDENTLSIDVSKIKYAIEEDTSAIVPVHVFGNGCEVEKI
    DMLAKKHNLKVIYDAAHAFDVKYKGESILNYGDISTLSFHATKIFHSIEGGALIINDDSLVEKVRYFINF
    GIESSESIPYLGTNAKMNEFEAAMGLCVLDDIIEIKSKRKVITEIYEAGLDGLVKFQEQNQHSSRNYSYF
    PVIFRTEEELLRVQKALIQNDIISRRYFYPSLDSLSYIEPKQYMPISRDISKRILCLPIYAELEDDKINK
    IINNIKEVSS
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Immunization with WbtI(G191V) protected mice against a relatively low-dose intraperitoneal challenge with LVS (F. tularensis subspecies holarctica live vaccine strain), but was less protective against a high-dose challenge (Li et al., 2007).
  • Additional Molecule Role : Virmugen
  • Additional Molecule Role Annotation : A wbtI mutant is attenuated in mice. The mutant protected against a low dose challenge with the parental strain, but was less effective against higher doses. However, increasing the immunization dose increased the resistance to higher challenge doses (Li et al., 2007).
  • Related Vaccine(s): Francisella tularensis wbtI mutant vaccine
1. Ccl2
  • Gene Name : Ccl2
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 20296
  • NCBI Protein GI : 6755430
  • Locus Tag : RP23-350G1.3
  • Genbank Accession : AJ238892
  • Protein Accession : NP_035463
  • Taxonomy ID : 10090
  • Chromosome No : 11
  • Gene Starting Position : 82035576
  • Gene Ending Position : 82037451
  • Gene Strand (Orientation) : +
  • Protein Name : chemokine (C-C motif) ligand 2
  • Protein pI : 10.38
  • Protein Weight : 14837.89
  • Protein Length : 148
  • Protein Note : Also known as JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594
  • DNA Sequence : Show Sequence
    >gi|372099099:82035576-82037451 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
    TGCAGAGAGCCAGACGGGAGGAAGGCCAGCCCAGCACCAGCACCAGCCAACTCTCACTGAAGCCAGCTCT
    CTCTTCCTCCACCACCATGCAGGTCCCTGTCATGCTTCTGGGCCTGCTGTTCACAGTTGCCGGCTGGAGC
    ATCCACGTGTTGGCTCAGCCAGGTGAGACCCCCCCAGCTCCCTTCCCAGCATATCACCCCATTTTTGAAT
    TGTCGTGGATTGTGATAGCATAGTCTCACATGGTCAGGTACTTTTTTTTTTTTCTTTAACCAAGATAAGG
    AGCATAAAGAAGGAAGGACAAAGAGCCAACCCAATTACAAGATTGCTTCTGGAAAGCAACTAGAATTTTA
    ATTGTTAGATCTAAATTTGGAATCACACCTTCATATAGTTCCTGTTCCAGTTACTTCCCTCAGTATTTGG
    GAACCTGGGTGATCAAACAGAGGCTTGGGTTGGTGCCTTTTTCCAGATAGAGGAGAAAGGGGAAGAGATC
    CAAGATCCGAGCTGTGTTTCACCCAGCCCTGCTTCCAGAGATAGCAGCTTAGCGGAGGTGGTTGGGATCA
    GAGATACTCATGATTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAGCCTACAGTAATGTA
    CTCAGGTAATCTTCTCAGGTCATAGTAATTTGACTTCTAACTCCCCCAAATGACAGTCCCCAGAGTCACA
    TAGTTTTAATGGCATCCCTCTACCCAAGACTGTGAGCCTACTTTAAGCTGCAAATAACTGAGTCTGTTGT
    CAAAGATCACATCCCAGATCTGATGTATTGGCATTTATATCCCATCCTGCTGAAACTGCCTTCTCCCCGT
    GGTCCTTCTCTTCTCTAAGGTCAGAAGCATCTTTCCTGTCCTAATGTGCTTCTCTTTTACTCTCCAGATG
    CAGTTAACGCCCCACTCACCTGCTGCTACTCATTCACCAGCAAGATGATCCCAATGAGTAGGCTGGAGAG
    CTACAAGAGGATCACCAGCAGCAGGTGTCCCAAAGAAGCTGTAGTGTGAGTTACATACCCCGGCCCTCCC
    TGGTCCAAAGGTTTTTCCTTAAGAACAAGGGATGGTCCTCATATACTTATAGTCAGTCACACACTCAGAT
    CCAATGGGGAAACCAAGGCCAAGAAGGCAAAGGCAGTTCTCAACAGCATTGTCTCTATGGCTGCTGTTCA
    GGCCCTTTCTACTCCACAAGCTTATCTTAGAAAACCTGCAGGAGAAGCAGGTCACTTTGAGTCCCCTTTT
    TCTACCTGCCCTCCCCCGCTGAGCTCTACACAGCCCCTCCATGTATACCAGACTGAACTTCATCTAACAG
    TGTCTTTTCTCTTCCCACAGTTTTGTCACCAAGCTCAAGAGAGAGGTCTGTGCTGACCCCAAGAAGGAAT
    GGGTCCAGACATACATTAAAAACCTGGATCGGAACCAAATGAGATCAGAACCTACAACTTTATTTAAAAC
    TGCATCTGCCCTAAGGTCTTCAGCACCTTTGAATGTGAAGTTGACCCGTAAATCTGAAGCTAATGCATCC
    ACTACCTTTTCCACAACCACCTCAAGCACTTCTGTAGGAGTGACCAGTGTGACAGTGAACTAGTGTGACT
    CGGACTGTGATGCCTTAATTAATATTAAACTTATTTAACTTATTGATGTTATGGTATTCCCTTTCATGAA
    TACTAAAATTTCTTAAATGCAAGGTGTGGATCCATTTTTCCCTCTCTGTGAATCCAGATTCAACACTTTC
    AATGTATGAGAGATGAATTTTGTAAAGATGAATGGGTAAACTTTGTGTTTGAGATTCCAAGGTATTGTTT
    AAAATATTATTATGGATATTCCTTATTATTAAAAGAAATATATTATTTTTGTACAC
  • Protein Sequence : Show Sequence
    >gi|6755430|ref|NP_035463.1| C-C motif chemokine 2 precursor [Mus musculus]
    MQVPVMLLGLLFTVAGWSIHVLAQPDAVNAPLTCCYSFTSKMIPMSRLESYKRITSSRCPKEAVVFVTKL
    KREVCADPKKEWVQTYIKNLDRNQMRSEPTTLFKTASALRSSAPLNVKLTRKSEANASTTFSTTTSSTSV
    GVTSVTVN
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis FTL0552 mutant vaccine
2. Ccl5
  • Gene Name : Ccl5
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 20304
  • NCBI Protein GI : 164698428
  • Locus Tag : RP23-320E6.2
  • Genbank Accession : AB051897
  • Protein Accession : NP_038681
  • Taxonomy ID : 10090
  • Chromosome No : 11
  • Gene Starting Position : 83525777
  • Gene Ending Position : 83530517
  • Gene Strand (Orientation) : -
  • Protein Name : chemokine (C-C motif) ligand 5
  • Protein pI : 8.73
  • Protein Weight : 9475.12
  • Protein Length : 91
  • Protein Note : Also known as SISd; Scya5; RANTES; TCP228; MuRantes
  • DNA Sequence : Show Sequence
    >gi|372099099:83525777-83530517 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
    CTTTGTCCTTGCATCTGAAATTTTAATGAAATGCTGATTTCTTGGGTTTGCTGTGCAGAGCCTCGGAGCA
    GCTGAGATGCCCATTTTCCCAGGACCGAGTGGGAGTAGGGGATTACTGAGTGGCATCCCCAAGCTGGCTA
    GGACTAGAGCAAGCAATGACAGGGAAGCTATACAGGGTCAGAATCAAGAAACCCTCTATCCTAGCTCATC
    TCCAAATAGTTGATGTATTCTTGAACCCACTTCTTCTCTGGGTTGGCACACACTTGGCGGTTCCTTCGAG
    TGACAAACCTGCAGGAGAGAGGAAGGTCAACACTGGGGCAGCGTTTGGTGTACGGGCACGGAAAAGCATA
    AAGGTGACAAGAGCCGGGGAGTGCGTTTTTGTAGCTCCATACTGTTTTCTGCCTTTTAGAGGGTAAGTCT
    TCTCCTTTGGAACCAGAGGCACTGAGCTACAGGCAGAGTTCCATTTGGGTTAATTCCCTACAAACACACA
    GATTTGGAACCCAGCATTGAGCAGGCCAAGCTCTGGCCTTTTCTTGGCCCCTTTCCTTGCTCCAGGCCTT
    ATGCTGAGTGACGAATTTAGAGACGTTGCGTTTGATTCACTTAAATCAGTTGAGTTGAGGAAATTAAGAG
    GGAACTCTAAGACTGCCTAGTTGTTGCTCTTGGCATTCTAAGCCTGTAGCCAGGGAGTCAAAAGGCTAGG
    ATACCAACTCCAGCTATCAAGATTGCTCAGGCCCCCATTTCCCCGTCCTTTATACAGTATGTGATACCAA
    AGAACAAATCTCCTTTGCTAAGTCTTTCCTTCTTTCCTCCACTTTTGGAGCCTCTGTTCATCTGACTTCC
    CCTTCCACCTGTCTCTTAAGAGCAGGTGTCCTCAAAGAAATTGTCCCCCTTACCTTCTCCCTTTTCCAAT
    CTCTGGCCCCAGGCCTGCATATAGTTGATGCTTGGTGAATATTGGATGAATGACTGGATGAAAGAATCAC
    ATTCATTCATTCTTACCACATGTATGCATTCATGTGCGCACGCACACACACACACACACACCTGTGCACA
    CATGTGTGCGCACACACACAGACACACAAATAATATCAATAGTAAGTTGATACTTCTTTATACCCATTAT
    GTGCCAGGGAATGGTCTAAGTGATTTTTTTTTTCTGTGTCCTAGTGAAAGAAATGCCTTAATTATCCTCT
    TTTGGAACTACCTCAGAGAGGTTGAGGGGCCCCAGTTTATCAAACAACATAACTAGGATTCTAGCATGCA
    GACCAGCTGGGTGGCCAAGGATGACTAAGACAATGTTGCCCAGCCTCTTTTATTGGCAGTTCCTGGACTC
    TGTCTTCAGCCCTTGGGTTGCACTCCATCTGGCATCTCCATGGATTATCTTGCTGGAACTTTGCTTGGTT
    CCTTCCTCTTTACACAGTTGGCCCTCTAGACCTGCACACCTGCACACCTCTCCGTCTCTGACTGGGAACC
    TCAGCATCTGCATCTCTTCTGTCCTTGCTCCTGACCACAAGTCCCTCACTCTGGCTGACTCATCCTCCCA
    CACTCCTGACTCTTGTTTCTTCTGGCCTCCAGGTACCACCACTCTGCTGACAATAGTACACGTGTGGGTG
    ACTTCAACCGCCTCTTCTCCAGACCCCCTCCTAGGCTTTGTCTGGACCTGGTCATTTTACTCTTGCCAGC
    TCTAGGTTTTGCTTCAGTTTCCTGACACTTGAGGTGCCCTCTGCTTTCCTACATATGTCCCTACAAACCA
    ACGTAGGTGTCCATGTGTTCTCAGATACACCCGTGTCACACACATCAGATACACCCATGTCACACACACT
    TCTCTACTCCTAATCTTGACACAAATAGCATCTTTTTACAGTAATCACCTGCCTCACCCTTGAAGCTAGA
    CACACCCCCTCCTCTCCTCTAGTATGGTTTTATAGGGTGCCGTAGATCATTCTGTGTCCAACTTATCAGT
    CTTACTAGATGAAATTTCCATAGCCAAGGATGACAATTTTATTCATCTCTGCCGGCTCATAATAGGTTCT
    CAAGGAAGGATTTTCTTTAAAAAGTTTTATTTTAGAGCTAAATTTGGTAATATTAAATAGTAATATTACA
    CTGAAGTTCATCTTTGCCAGGGTTAAAAAAAGTCATTGGATTAAAACTCTTCAACCTTTTATTGGAAACT
    TTATGATTTCTTCTTTTTCTTTTTTACATTTATGTAGATGAGTATTCATCTGCATGTATTTGTGCACCAC
    ATGTGTGCCTGAGGAGGTCAGAAGAGGGTATTGGATCCCCTGGAACTGGAGTTACAGAGTGAGCCATTAT
    GTAACGAGTGAGCCATCATGTAACCAGTGAGTCACCATGTAACGAGTGAGCCGCCATGTAACGAGTGAGC
    CACCATGTAACCAGTGAGCCACCATGTAACGAGTGAGCCACCATGTAACGAGTGAGTCACCATGTAACGA
    ATGAGCCACCTTGTAACGAATGAGCCAACGTGTGGATGTTGGGATGGAGGTTGATCCTCTGCAAGAGCAA
    GCAGTGCTCTTAACCATGGAGCCATCTCTCCAGCTCTCCGTTTTCACTCTGTAAATCGTTATCATCTTAA
    GCTTCTTAAGCTATCCTGTCACTAATGACTTTATTTTAATACAGGAAGTAGGTCCAGAGACAGACTGCAT
    CCCTCACTTACTGTGCAGCTTTGAGTAGGCCTGCAAATGCCTCTGAATCTGTTTCATTACGTGGAGAAAT
    TTGTGCATACCCGCCAGGGCTATGCACAATTTAGAGACAGTACACATAGACACAACATGCCAGCAAGCAC
    CCTATTATATTCCCCAAATCTATCAATATGCATATTTAAAGCTGAGTCTCATTATAACTTCTTAAATGGA
    TAGGGTTTTAGATTTTATTTACTTGATTGAAATTAGGGAACTGACCTTGATCCATCCTCCCTAGTTCCTC
    AGAGGATTAGGCAAAGGTTGGTGTCCTAGGAACTGTGTCTATCTCTATAGGCAGGAGCCTCTAAGGCTAC
    GGTTCTCAACCTTCCTAATGCTGCGGCCCTTCTCATGTTGTGGTGACCCCCAACCATAAAACTATTTTTG
    TTACTGTTTCATAATTGTAATGTTGTTACTGTTATGAATTGTAATGTCAATATTTTTGGAGATAGAGATT
    TGCCAAAGGGGACCCGACCCACAGATTGAGAACTGCTGCTCTAGGGCTCCCTGTGTCTAGTCCTTTAATG
    TTACAGTTGGAGACTCTGAGCTGGAGACGAGGAGCCATAGCTATTAGAGGAGCCCAGAGAGTGAGTGGGA
    GTGAGAGGGGGCACCCCTGAGAAGGATCACGGCGGGGTTGTGGGATGGCTTCTATACCCCACAGAGGAAC
    CCATGGGGCTGAGACTCACACGACTGCAAGATTGGAGCACTTGCTGCTGGTGTAGAAATACTCCTTGACG
    TGGGCACGAGGCAGCGCGAGGGAGAGGTAGGCAAAGCAGCAGGGAGTGGTGTCCGAGCCATCTGGGAGAA
    GGAAGAAAGGAAACCCTTTTATTCATTGCCAGCTATGCTGGGCTCTTTACACGATTTATCTTAAAAGCAG
    TGACTGTCGTTGTCGACCTTGGCAGAAATGGCTCAGAGGACACATTTGCCATGGTGGAGAGTTCAACAGA
    AGCCTGTTTTGTCTGGTCCGTGAGCCAAGCCTGTGCCTTGTGCTCTGTACCGTAAGCCCCTGCTGGGATC
    CCTCTCCTCGCTCTGTGCCTTCTGCCCTTAGCAGGGCTTTCAGAGCTCCCCTGGTGGATTCAAAGCTTGG
    TGTCTTCGAGGTGTAGCATGAGTTCTGGGGGAGTGCCCAAGCCGAAGCTCCATAACTTAGTGACGATCCT
    AAGAGGGGCCTGAAAGTGCACATAGCATCCTGGGACCTGGCTGGCCTGTAGCGAGGGGCTCTGACCAGAG
    AAGCTCTTGGTGGTCACTTAATTCATCCCACAGCAGAGCTGTGGTGCTTAGCTTCCCAGTCAGATAGGGC
    TTTTGGAATACCAGTTTCCACAATTGTAGCAGTGTGCACCCTTAGACAAGAATCTCAGTGTTCTTAGGCC
    TCTGTTTCCCACTGCAACACAAGAGCAACTACTGAAAGACTATTTGTATTAAACAGGATGGCACATTAAA
    CTCATTTGCACAGTGCCTGACCCACGGTAGGTACTTAGTTCACTATCTATCTATCTATCTATCTATCTAT
    CTATCTATCTATCTATCTATCTACCTATTTATTTTGCAAGCCCCATTTCTTTTCTCACTATGAAATGAAA
    GACCATCCTACCTAAAACAGTATCACACATTTCTTAGAAACCACACTGGTGACAGATATGAGCAAGTCAT
    TTTTCAAAGGTCAGCCCGGCACCAGTTCACAATAAGGAGTGCCTGCAGAATTCATAATATCATCTTTGAA
    GACACAGAAAAGTTCCTCAGAGGATCCTGCCATTAGTATACAAGACATCTAACAGTGATGACAGGGTAGC
    CGTGTCAAGGATGCGTAAGAATGTACAGCAGCATACACAGTAGGATTGGAAAGAAGGGGAGGTCTGGGTA
    TCAAGATGGGTTGGCATCGAGGCAGAGTGGGGCTGTGATGGTCAGAACCCTCGACTTACATGGTGAGGCA
    GGTGCGGGGGTGCAGAGGGCGGCTGCAGTGAGGATGATGGTGAGGGCAGCTGCAGAGATCTTCATGGTAC
    CCGCGGCAGAGGCTGTGGGAGATGCATGTGCTGTCTCAGAGTCCTCTGCAA
  • Protein Sequence : Show Sequence
    >gi|164698428|ref|NP_038681.2| C-C motif chemokine 5 precursor [Mus musculus]
    MKISAAALTIILTAAALCTPAPASPYGSDTTPCCFAYLSLALPRAHVKEYFYTSSKCSNLAVVFVTRRNR
    QVCANPEKKWVQEYINYLEMS
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis guaB mutant vaccine
3. Cxcl1
  • Gene Name : Cxcl1
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 14825
  • NCBI Protein GI : 6680109
  • Genbank Accession : AC157938
  • Protein Accession : NP_032202
  • Taxonomy ID : 10090
  • Chromosome No : 5
  • Gene Starting Position : 90891244
  • Gene Ending Position : 90893120
  • Gene Strand (Orientation) : +
  • Protein Name : chemokine (C-X-C motif) ligand 1
  • Protein pI : 9.62
  • Protein Weight : 9757.89
  • Protein Length : 96
  • Protein Note : Also known as KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
  • DNA Sequence : Show Sequence
    >gi|372099105:90891244-90893120 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
    CAACAGGACTCAGAGCCTCTAACCAGTTCCAGCACTCCAGACTCCAGCCACACTCCAACACAGCACCATG
    ATCCCAGCCACCCGCTCGCTTCTCTGTGCAGCGCTGCTGCTGCTGGCCACCAGCCGCCTGGCCACAGGTA
    GGTCTCGCCACTGCCACTGGGGGAGGAGGGACCTCTGGTGAGCGCAGCCTCCCACAGTCCCGCTGACCAA
    GAGTCTTCTCCCATAGGGGCGCCTATCGCCAATGAGCTGCGCTGTCAGTGCCTGCAGACCATGGCTGGGA
    TTCACCTCAAGAACATCCAGAGCTTGAAGGTGTTGCCCTCAGGGCCCCACTGCACCCAAACCGAAGTCAT
    GTGAGTATCTTCCCGGTTAGCTTCTGCCACTTCCAGACTCGCCCAAACCCTCCCGCGCCCCCACACTTCT
    CCTAGTGGGAATGCCTAACATGTGGGTCTATCCTTCTCTCTGCAGAGCCACACTCAAGAATGGTCGCGAG
    GCTTGCCTTGACCCTGAAGCTCCCTTGGTTCAGAAAATTGTCCAAAAGATGCTAAAGTGAGTTGTGACTT
    GTGTTTGTACCCGAGACTAGAGTCAAGGGTGGGAGTACTGGCATATGACCCCTGAACGTTAATTCTATCA
    AGGAAAGTCTGTGTTTATCAGGAGGTCGGAAAGTTGTGGTTATTATTTTGGTAATTGACCTTAATTTGAG
    ATCATTAATTGGTTCTGATGCTAGAGAATTGTGTCCTCTTGGACACAATTACTATTGTATATTACACAAT
    TACTATTGTGTATAATTATTACCACACTCAAAACCCCTATGCAAATGGTCAGTGGCAACAGTTCCTAAAA
    GATGGAGAAGTCGCCAATAGCCTTTAAAAGTCTCCTTAGGGGCTGGCAAAGCATGCCCGAGCCTTCTTTT
    GCCAGTGAGATTTCCCTGAGTTCAAGGCTAAGGTCTCTGTGAATGTTACATAATTTATTGAATTTAAGCC
    AAGTAGGGGAGATAATAGCAGCTATATCACAGAGTTGTGAGTGAGAGAGGATTAAAGTCACACTTGTAAA
    TTAACCCATGCTGAAGTAGGTTTTTGTTTGTTTGTTTTTGTTTTTGTTTGTTTTTGTTTTCCAAGTCAAG
    TGATTGACTCCACAGGAACCGACAGTACAGAACTCTGGGTGCAAGTGTAGACATGTAGAAAGCCCATCTG
    GAGCCTCAGGGGCTGGAATAAAAGAAGAAAGGCTTTGATGACTATTTGTAACAATGACTCTTTTCTGATT
    GCAGAGGTGTCCCCAAGTAACGGAGAAAGAAGACAGACTGCTCTGATGGCACCTGTCTGGTGAACGCTGG
    CTTCTGACAACACTATACAATTTCTTTTGAGGGTCCTATTTATTTATGTATTTATTTATTCCACAAAGTG
    TGTGGTTTTTATTTTACATTAATATTTAACAGTGTGGATACATTTCATCGATGGTAGTTCAGTTCTGCTT
    GTTCAGTTTAAAGATGGGTAGGCTTAAAATATTTCATTAAACTAATATTTATTGGGAGACCACTAAGTGT
    CAACCACTGTGCTAGTAGAAGGGTGTTGTGCGAAAAGAAGTGCAGAGAGATAGAGTTTAGTATTATGTTT
    TGTATGTATTAGGGTGAGGACATGTGTGGGAGGCTGTGTTTGTATGTCTTGAAAAGAATGTCAGTTATTT
    ATTGAAAGTCGTCTTTCATATTGTATGGTCAACACGCACGTGTTGACGCTTCCCTTGGACATTTTGTGTC
    TAGTTGGTAGGGCATAATGCCCTTTTACATTCTTTAACCCTGTTTCTCCTGGTCTCGTCTCGCTCGGGAC
    AGAGACGTTCAAAGGACTGTTACAAATGAAGTAAAAATAAAAGTTTTATTAAAAAAA
  • Protein Sequence : Show Sequence
    >gi|6680109|ref|NP_032202.1| growth-regulated alpha protein precursor [Mus musculus]
    MIPATRSLLCAALLLLATSRLATGAPIANELRCQCLQTMAGIHLKNIQSLKVLPSGPHCTQTEVIATLKN
    GREACLDPEAPLVQKIVQKMLKGVPK
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis guaB mutant vaccine
4. Ifng (Interferon gamma)
  • Gene Name : Ifng (Interferon gamma)
  • Sequence Strain (Species/Organism) : Mouse
  • NCBI Gene ID : 15978
  • NCBI Protein GI : 33468859
  • Genbank Accession : NM_008337
  • Protein Accession : NP_032363.1
  • Other Database IDs : MGI:107656; UniProt: P01580
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence
    >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
    ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
    GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
    GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
    AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
    AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
    GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
    AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
    GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
    AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
    AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
    AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
    TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
    TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
    ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
    GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
    AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
    TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
    AGTATACCTTTGCAACC
  • Protein Sequence : Show Sequence
    >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
    MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
    ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
    LLPESSLRKRKRSRC
  • Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
  • Additional Molecule Role : Vaximmutor
  • Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
  • Related Vaccine(s): F. tularensis FopB Protein Vaccine , Francisella tularensis FTL0552 mutant vaccine , KKF24 , LVS
5. IgG
  • Gene Name : IgG
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16059
  • Genbank Accession : AF010213
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 881411
  • Gene Ending Position : 914690
  • Gene Strand (Orientation) : +
  • Protein Name : immunoglobulin heavy chain (V7183 family)
  • Protein Note : Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)
  • DNA Sequence : Show Sequence
    >gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
    CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
    GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
    GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
    TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
    TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
    CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
    CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
    ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
    CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
    CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
    AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
    CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
    AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
    TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
    AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
    AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
    ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
    TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
    GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
    TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
    AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
    TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
    GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
    TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
    TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
    TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
    ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
    CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
    GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
    CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
    TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
    GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
    TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
    CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
    AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
    TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
    CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
    AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
    TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
    CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
    AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
    AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
    TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
    AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
    ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
    CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
    ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
    GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
    ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
    AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
    ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
    ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
    CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
    CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
    CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
    GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
    ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
    CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
    CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
    AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
    GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
    TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
    AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
    GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
    GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
    ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
    TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
    AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
    ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
    CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
    TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
    TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
    TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
    CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
    AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
    ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
    CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
    AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
    GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
    ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
    CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
    TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
    TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
    TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
    CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
    AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
    AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
    CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
    CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
    TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
    AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
    CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
    ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
    CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
    AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
    TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
    TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
    GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
    GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
    AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
    CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
    AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
    GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
    ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
    TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
    TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
    GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
    GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
    GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
    TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
    TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
    AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
    GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
    GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
    CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
    CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
    ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
    CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
    ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
    GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
    GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
    GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
    TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
    TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
    CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
    TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
    GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
    TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
    CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
    AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
    TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
    AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
    CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
    CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
    TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
    GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
    GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
    ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
    GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
    GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
    GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
    GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
    ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
    TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
    AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
    AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
    AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
    GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
    CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
    GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
    TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
    TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
    CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
    AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
    TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
    TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
    AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
    TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
    GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
    CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
    TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
    TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
    TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
    AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
    GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
    AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
    TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
    GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
    TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
    TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
    TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
    CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
    CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
    TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
    ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
    TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
    ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
    TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
    ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
    AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
    TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
    AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
    CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
    CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
    GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
    CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
    GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
    TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
    CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
    TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
    AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
    GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
    ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
    GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
    TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
    CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
    CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
    ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
    GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
    TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
    ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
    CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
    ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
    TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
    GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
    TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
    GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
    TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
    GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
    ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
    CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
    CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
    ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
    AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
    ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
    AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
    GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
    TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
    GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
    GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
    TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
    CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
    GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
    TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
    CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
    TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
    TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
    TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
    GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
    CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
    AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
    GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
    ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
    TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
    AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
    TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
    AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
    CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
    GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
    TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
    TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
    TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
    GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
    ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
    ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
    CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
    GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
    TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
    CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
    GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
    GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
    AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
    CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
    TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
    GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
    ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
    TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
    AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
    AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
    ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
    TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
    GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
    TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
    TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
    TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
    AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
    ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
    AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
    GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
    CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
    AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
    TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
    TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
    TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
    GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
    GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
    ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
    AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
    AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
    GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
    TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
    GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
    ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
    AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
    TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
    GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
    CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
    TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
    TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
    GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
    ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
    CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
    ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
    TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
    TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
    TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
    CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
    CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
    GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
    AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
    AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
    GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
    ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
    TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
    TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
    CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
    TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
    AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
    TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
    AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
    TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
    GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
    TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
    ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
    ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
    CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
    TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
    TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
    TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
    TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
    TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
    GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
    CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
    AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
    CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
    CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
    CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
    GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
    TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
    CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
    GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
    TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
    GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
    TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
    AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
    TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
    CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
    AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
    CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
    TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
    GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
    CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
    ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
    ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
    TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
    GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
    CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
    GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
    GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
    GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
    ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
    TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
    CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
    TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
    TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
    ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
    TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
    GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
    CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
    TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
    CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
    GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
    ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
    CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
    CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
    GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
    AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
    AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
    ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
    ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
    GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
    ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
    CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
    CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
    TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
    CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
    CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
    TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
    TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
    GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
    TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
    AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
    TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
    GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
    CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
    CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
    TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
    GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
    GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
    CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
    AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
    TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
    CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
    TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
    AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
    TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
    CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
    AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
    TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
    CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
    TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
    GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
    ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
    TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
    TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
    AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
    CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
    TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
    GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
    GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
    TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
    TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
    AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
    AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
    TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
    CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
    ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
    ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
    ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
    GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
    TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
    ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
    TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
    GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
    AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
    TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
    TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
    TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
    GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
    AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
    CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
    TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
    GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
    CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
    ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
    AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
    CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
    CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
    ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
    GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
    ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
    AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
    AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
    GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
    CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
    AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
    ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
    ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
    CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
    TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
    CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
    TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
    CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
    AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
    GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
    GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
    GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
    TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
    TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
    TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
    ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
    ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
    AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
    TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
    CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
    CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
    TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
    AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
    CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
    CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
    GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
    ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
    CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
    ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
    TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
    ACAAGCACAGTGCAAATTTCTGTCACCCTG
  • Molecule Role : Vaximmutor
  • Additional Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis capB mutant vaccine
6. Ighg1
  • Gene Name : Ighg1
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16017
  • Genbank Accession : AC160982
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): KKF24
7. Ighv1-9
  • Gene Name : Ighv1-9
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 668478
  • Genbank Accession : AC073561
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 114583568
  • Gene Ending Position : 114583861
  • Protein Name : immunoglobulin heavy variable V1-9
  • Protein Note : Also known as Igg2a; Gm16697
  • DNA Sequence : Show Sequence
    >gi|372099098:114583568-114583861 Mus musculus strain C57BL/6J chromosome 12, GRCm38 C57BL/6J
    GTCTTGCACAGTAATAGATGGCAGAGTCCTCAGTTGTCAGGCTGCTGAGTTGCATGTAGGCTGTGTTGGA
    GGATGTATCTGCAGTGAATGTGGCCTTGCCCTTGAACTTCTCATTGTAGTTAGTACTACCACTTCCAGGT
    AAAATCTCTCCAATCCACTCAAGGCCATGTCCAGGCCTCTGCTTTACCCACTCTATCCAGTAGCCAGTGA
    ATGTGTAGCCAGTAGCCTTGCAGGAAAGCTTCACTGAGGCCCCAGGCTTCATCAGCTCAGCTCCAGACTG
    CTGCAGCTGAACCT
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis capB mutant vaccine , Francisella tularensis wbtA mutant vaccine , KKF24
8. IgM
  • Gene Name : IgM
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16019
  • Genbank Accession : AC073553
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 171229
  • Gene Ending Position : 175133
  • Gene Strand (Orientation) : -
  • Protein Name : immunoglobulin heavy constant mu
  • Protein Note : Also known as Igm; muH; Igh6; Igh-6; Igh-M; AI326478; MGC107036; MGC107507
  • DNA Sequence : Show Sequence
    >gi|121699722:171229-175133 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
    GTCATTTCACCTGCAGGTGACAGAGACAGTGTGAATTGCTGTCCACTTGTAGGGAGTAGAGGTAGAGGCA
    GTGAGGACTGGTGACTCCCTGTCCCAGCCCTGTGTCCTCAGCCCCACAACCACACTACCTTGAACAGGGT
    GACGGTGGTGCTGTAGAAGAGGCTCAGGAGGAAGAGGACGATGAAGGTGGAGGCAGTGGTCCACAGGTTC
    TCAAAGCCTTCCTCCTCAGCATTCACCTCCCCCTCTATGAAGGGACACAGAGAGGGAGGGTCAAGCCAAG
    TCTTGGGGTGACTGAGCATTTGCATAAGGGTTGGGGTGCCCTGATGGTTCTGGAGAATCTTAGCGAGTAT
    TGACTGGTCCCCTAATAGCTCAAGCTAGACGGAGGCACAGAACAGGCCAAGCTGTAGGACACACTAATTT
    GGAGCCTTTGTTCCAGGAGCCCTGGGGCAGCAGAGCAAGCAAGACAGATGCAATGTATGGACCTGGGTAA
    AGGCAGACTCAGGAACTGTGGCAAAATGATATTGGTGTGCTCTGGACTTTCCCTGAGCAGGTGGTGCCAT
    CGGTGCTGTTTAGACTGAGAATTTAGAGCAGGTCTCCCTGTCTCTTGGGCTTTAGCAATAAGAACAGGGA
    TACCAGAGGTTGTCCCTCCTTACTTCCTGAAGGACTCAGGCATCTCATGACTTTCTTTGATCAGAGTTCC
    CGTGCGTTGCTTGTCCTACCCCTGCCAGCAATGTTGGGTCTTGGTACCAAGAGATAAACAGACAGATACC
    TGAGGGAGGCTGTGGGACCAGGGCATTTATTGGGATCTCTGATCCCTGTAAAGATGAGCCCTTGGGGATA
    TCTAAAGCCTAGGCCCCAAATGGATATGAATATGAAGAATGTCCAGGATATGAGCCCAAAGAGGCAGTGT
    TATTGGGTGGTGACTCCTTACAGAGGCTCCTTGATGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
    GTGTGTGTGTGTGTGTGTGTGTGTGTGGTTGGGGCATCTTTTGGACAACTGCATTATGTTTTCTGCATTT
    TGAACTGGAGATCAGGGTGGGTGTGAGTGGGTTAGGCAATTTCACATTGGTCTTCTGAGGTGTTCTATTA
    ATGGAGACACAGCCAAGGCTAACTTTCTGTCATGGGTGGTTAGTGGAGTATCTTCTGCCCCATGGTGTTG
    AGACAGATAGGCATACTTTGTACCTGGGGTTATCTCTCCCAATCATAGAGTGGGCTCCTGAGGAGTATCA
    GTGACCCAGCCTAGTGTAGGCTCTCTGAGCCGTGAGCCTTGGAGATTACAGCCCTGGTCAGAGTCCAGGT
    GTATCTGTGTGCTATACCTGTTCACATGTTAGTGTACACATGTGAAGATGTTTCTGTACATGTGTGTATA
    TGCATATCTTTGTATCTATATAGTTTATTTGTGTATATTTGCCTATAGTCTGTTTCTGTGTACACAGGAC
    CATGCATATTGGTATCTGTTTCTGTGAGTGGTTGTGTGTCTATGCCTATTTGTCTCTGAAGGCGTTTGTA
    AGTGTCCATGCATGTGTGTGCGCATGAACTCATGTGTATATATGTGTACCTTTGTCTGTGTGTCCATGAA
    TACACATTCATGTATGTGTCTACACATGTGTACCTATATATCTATGTATAAATGCATGTGTGTCTATATA
    TGTGCCTGAATGCTGCCTGCACCAGGTTAGGCAAGCTTTCAGCCATATATCTAGAAGTAGCTGGAGGAAT
    CGCATGTTCATACTCTGTCTGTTGTAAGCCTGACTGAGTTCACACACAAGGAGGAAACTGGCAAGGTAGA
    GGAAGGGCCAGGGTGGAGGCAAGTATGCAGGGTGTGTGCAGAGCAAGCAAAACACAACTGTCTCAGCAGG
    CATGAGCATTGTATAATCAAAACCAGTGACGTTTGAATGGATTTTTTTTATTTCTAATTTTTATAAAATG
    CAACATCTCACTCTGACATGGTTAGTTTGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGA
    GCGCTAGCATGGTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTACAGTGTGGGT
    TTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGTGGCAGGGCCTCGTGGCCTACAACACAGG
    TATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGCTGTGGGTAAAGTAGAAGCCTGG
    GGCCCCAGGCTCTGGCATCGGGGCACTGGTCACATACTTCTCTTGGGGCAAGAGTTGCCCTCTCTGAAGC
    CACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAGGCAGGTGACTGTGGCTGACTCCCTCAGGT
    TCAGTTGCTCACGAGCTGGTGGCAGCAGGTACACAGCAGGTGGATGTTTGTGCACCTCTGTAAATGAAGA
    CAGTAGTGAGGATAGGGTGGAAGAGGACCTGCCCTCCCTATGAGGTACAGGAAGGGTCCTGCAATTGGAG
    GGGAAGGGAAGGGGGGATACCTACCATTGGGTTTTGAGATGAATTTCTTCTGTGGTGAAGGCAGATCCCT
    GTGAGTCACAGTACACACAAATTCCTTCCTGTTATTCCAGTCTTCCACACAAACACTAGCCACACCCTTA
    GCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTTGGTTTCCAGTGGTTCACCACTTTGAG
    AAGCCCAGGAGATATTCAGGGTTTCATAGGTTGCCAGGTTTGAGACCAGACAGGTCAGGTTAGCGGACTT
    GCTGAGGAAGATGTCGGCAAAGGAGGGGGGGATGGTGAAGGTTAGGATGTCTGTGGAGGGACCTGCAGTC
    AAGAGAACACTGTGTCAGGTTATGACATATCTAGTTGTGAAGTTGGAAAAAAGCTTAGTCTCCATCCCCC
    AAACCTACTTAAGGGAAGATGCTGGTCCTTCCAGCTGATTCTCACCCCTCTACTGCCTATTGCTCATTCT
    TGTCCTTGGCCACTCTGGGGAGCCAAAGTTCAAGGAGCAAATGACCATGTCTGGGTGGCATTGGCCATAA
    TTGTAGGAGGACTTCCCTAATCTGGGAGGGCTAGGCATTGGGCTTAGCCCAGGCCACTCACTGGCAGCAC
    ATGTGGAGGACACGTTCTTCAAGAAGGTGAGACCCCTGTGATCCACACGGCAGGTGTACACATTCAGGTT
    CAGCCAGTCGATTTCAGAGATGGTAAGTGTGCTTATGACCTTGTAGGTTTGGGGTGTGGATCCTTTGTTC
    TCGATGGTCACCGGATCTGTGGTGAAGCCAGATTCCACGAGCTTCCCATCCTTTAGCCAGGATACTGTGA
    TCGGTTTTGGAGTGAAGTTCGTGGCCTCGCAGATGAGTTTAGACTTGCGTGGTGCAGGGCCAGAGAAGCC
    ATCCCGTGGTGGGACGAACACATTTACATTGGGGTTCATCTCTGCGACAGCTGGAATGAAAGGTCAAGGT
    GTGAGCTCAGCTTGGCCTAAGCCCCACCCCGCTGCTCCCTCTGGGCCATGCCTGGGCCTGGGCACCCCTG
    CTGGGAGGGTTTGGTTCTTACCTGGAATGGGCACATGCAGATCTTTGTTTTTGCCTCCGTAGTGGATTTT
    GCATACCAGGTATTCATCTGAACCTTCAAGGATGCTCTTGGGAGACAGCAACACCTGCGAGGTGGCTAGG
    TACTTGCCCCCTGTCCTCAGTGTTGGGAAGGTTCTGATACCCTGGATGACTTCAGTGTTGTTCTGGTAGT
    TCCAGGTGAAGGAAATGGTGCTGGGCAGGAAGTCCCGGGCCAGGCAGCCCATGGCCACCAGATTCTTATC
    AGACAGGGGGCTCTCGCAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTC
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis capB mutant vaccine
9. IL-1b
  • Gene Name : IL-1b
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16176
  • NCBI Protein GI : 6680415
  • Locus Tag : RP23-384K11.2
  • Genbank Accession : AL808143
  • Protein Accession : NP_032387
  • Taxonomy ID : 10090
  • Chromosome No : 2
  • Gene Starting Position : 129364578
  • Gene Ending Position : 129371119
  • Gene Strand (Orientation) : -
  • Protein Name : interleukin 1 beta
  • Protein pI : 4.31
  • Protein Weight : 29045.55
  • Protein Length : 269
  • Protein Note : Also known as Il-1b; IL-1beta
  • DNA Sequence : Show Sequence
    >gi|372099108:129364578-129371119 Mus musculus strain C57BL/6J chromosome 2, GRCm38 C57BL/6J
    GTTTGTTTGTTTTAATGAAATTTATTTCATACTCATCAAAGCAATGTGCTGGTGCTTCATTCATAAAAAG
    TCTCATCCATATTATAGGAACCTATTTATTCTCAGCTTCAATGAAAGACCTCAGTGCGGGCTATGACCAA
    TTCATCCCCCACACGTTGACAGCTAGGTTCTGTTCTAGAGAGTGCTGCCTAATGTCCCCTTGAATCAACT
    TAAATAGATCAACCAATCAATAAATACATAAATAAATAGGTAAGTGGTTGCCCATCAGAGGCAAGGAGGA
    AAACACAGGCTCTCTTTGAACAGAATGTGCCATGGTTTCTTGTGACCCTGAGCGACCTGTCTTGGCCGAG
    GACTAAGGAGTCCCCTGGAGATTGAGCTGTCTGCTCATTCACGAAAAGGGAGCTCCTTAACATGCCCTGG
    GGAAGGCATTAGAAACAGTCCAGCCCATACTTTAGGAAGACACGGATTCCATGGTGAAGTCAATTATGTC
    CTGACCACTGTTGTTTCCCAGGAAGACAGGCTTGTGCTCTGCTTGTGAGGTGCTGATGTACCAGTTGGGG
    AACTCTGCAGACTCAAACTCCACTTTGCTCTTGACTTCTATCTTGTTGAAGACAAACCGTTTTTCCATCT
    TCTTCTTTGGGTATTGCTTGGGATCCACACTCTGCAGAAAAGTAGAAAAAAGAATTGTGTTAACGTCTGT
    GTCCGTGTGCATGTTGCATGTATGACAGAAAGGATAAAGCTACCACGGTAGACATATTCTCAGGGCTGCT
    GGAGAAGAGAATGAAAAAGAGGTTGTGGAACTTGAGGCCACATTCTTTACTGCTGAACCCCATCTTTCTA
    TAATCACTGAAGGAACCTTTGTTATATATATATTTTTTCTGAATCCTACCATATGGGTGAGAATTATATT
    AAAGCTCCCAAGGGTACCCAGTTTCCAGGCATTTTTGTCACAGTTCCCCCTAAATCCCTGGATAGAGTGG
    CATCAGCTTGGAAATGAAGCTTTTGTTAGGAATGTCACAAGTGCCAGTACAGGTCTGAGGATCGTTGGTT
    GAACCCTTTCACTGCATTCTCCAAACTGTTCTCTGTCAAAGGAGGAAAAACTGTCACCATATACAACTCT
    TTCATCCTTGATGCTACGACTTCTGTTGGGGGTGATAACGGTGGCCTGACATGACAACAGCATCAAAAAG
    GATTGATTTCCCATGGTGGCAGTGAGGTTTATGTGCACTTAAAAGTTATGCTAATTTTATTCTATATTTA
    TGTAAATCGTCAAGAAGACCTCTAATGTTTTCTGGCGGGTCAGGCAGGTGGATGTCTGGGAAGTAAGTAA
    AGGCAGAGTCTTCGGTGAGCAGTGGGGGAGGCTTCTCTACTGATGGACTCACCTCCAGCTGCAGGGTGGG
    TGTGCCGTCTTTCATTACACAGGACAGGTATAGATTCTTTCCTTTGAGGCCCAAGGCCACAGGTATTTTG
    TCGTTGCTTGGTTCTCCTTGTACAAAGCTCATGGAGAATATCACTGGAGAAAGGAGAGGAGTCTGGGTCA
    AGGGAACAGCAATGCAGGGTGGGCTCAAGCATTAGACGTGACAGGCAAAGCCTATGCAGTCCCCCGTGTG
    AACCCAGAATGAGCCTCCTCAGACACAAGATATGGACTAGCAGGGCTATACAGTCATAGTCATAGACAGT
    AGCAAGGTCAGTAGGCCGATCCAGGATTCCCTCAGGACCTCTTAGGAGCAGCAATGAAGAACGAAAGGTG
    TCTAACTGTTAAGCTCTAAGGGCTAAAGCTGTTATGTGGCTGGCAGGCTGTGTGTCCGTCTACGTGAGAA
    GTACAGTTTGGTCGCTATGAAAACAAAGCTGTGCTCCTAAAAGGACCTAGGGGCCAGCACGTTTTGAGGA
    AAATAGAGCAGGAGGCAGGTAGAACTCTATTAGCTGGGGAGAGTGGCTCCAAAAAAGAGACCCCCCCCAC
    TTTGATCTTAGCCACAGTACCTCAATTGGAGGCCCCTGGCTGCTTTTATGACTTTATCAAGAAATAAGAA
    TTATTCTTTTTAAAAGGTATAAAGCAATAAGATTCTTTTCACCTGGACCAGAGTAGACAGCTCCCTCCAA
    AGCTTCTAAGATCAGAAGCTAAAAGGAGACAGCACACACTGCTGCTGTTAGCTCTTGTCCCCTCAGCTGT
    GGTGGTGTGGTGCCAGGTCCACACCATCATCTAAAGAGGCCCTGACCGCGCTTGGAATGCTTGTTATTAT
    GGAAGAGAGATGTATGCTCCTGATAGTCAGCAAACCTCCCTTCAAGGAACTGTGTGTGTGGTATTGGTGG
    GAAGGATATCAGCACGAGGGATAGGACTGGGAACAAAAATGGACATATCAATTCTCTACCATCTACATTT
    CAGCCTCAAATGAGGGAAAAAGAACCCACAGGCAGTTATTGCATGTCCATCAAATGACTTCACACTAATG
    TGTTATCCCTATATGACTGGGCTGGAAAAATGGTCCTTTTTCGTTCTGTCTCTGTCTCTGTCTCTGTCTC
    TCTCTCCTTCCTTCCTTCTTTCCTTTCCCTTTCTTTTTCCTTTTCTTTTTTTGTATAATGACCATAGTGT
    TCCATTTTAAAACACAGGGACCCAGTAGGCTGCCAGGAGGCCAGAAAGGGAAACCAAGATGCTCCTACTT
    ACCTTGTTGGTTGATATTCTGTCCATTGAGGTGGAGAGCTTTCAGCTCATATGGGTCCGACAGCACGAGG
    CTTTTTTGTTGTTCATCTCGGAGCCTGTAGTGCAGTTGTCTAATGGGAACGTCACACACCAGCAGGTTAT
    CATCATCATCCCATGAGTCACAGAGGATGGGCTCTGAAAGTTCAAGAGCAGATGTTGTTTAGGCAGATAA
    TTAGAAATGCAAGCACTGTGAAGTGAAGCAGCAGAATTTGGCTTATTGAGCAACACCTAAGCATGTGCAG
    TCAAGATTCTATTCTAATTTTAGTGGGGGTATAAAAACACTGGGAGTGGGCTGGGCAAGAACATTGGATT
    TGGCATCGTGAGATAAGCTGCAGTGTTATAACCATTCACTAGGGACTAGCAGTAGTGGTCCTTGCATGAA
    ACGATGCCAGTAATAAGACCAGCTTAACTGGGAAATTGTGAGATTCACAACCTCTAGTATAAGCCAAAGT
    ACTTGGCAAGTCAATGGTTGAGTTGCAAGTGTAAGCATCATTAAAGCCTTTTTATAAGTTAATCAGCTCA
    AGATAGAACTGATTTGTTTGGAGGTTTCCTCATTTTCTTCTAATGGTTGGAATGTCTAGCACTAAGGGTC
    TTGCACTTGAGGGGCGTTGAGTTCTAAGAGGTGGGCTGAGAAGGACCAGAGCGAAGGTCTGCTGTCTGCT
    CTCAGTCCTCATTAAGTACCTTCTTCAAAGATGAAGGAAAAGAAGGTGCTCATGTCCTCATCCTGGAAGG
    TCCACGGGAAAGACACAGGTAGCTGCCACAGCTTCTCCACAGCCACAATGAGTGATACTGCCTGCCTGAA
    GCTCTTGTTGATGTGCTGCTGCGAGATTTGAAGCTGGATGCTCTCATCAGGACAGCCCAGGTCAAAGGTT
    TGGAAGCAGCCCTGTGGAGAAACCAGGCAGTGAGCACATCAAGTCATCAGACCAGAGGACTCCTACATCA
    ACCCCAGAGAACACCAAGGGCCCGGGAGATAAGTTCTTAACCACAATCCCTGTCTATGTGGAGCCCAGAG
    AATTTAATTGGCCTACCCAGGGGTACATGGCAACCAGACACAGGCTTCCTGGCATTCTCTATAGTAAACC
    TCATTTCTACTTTATCTTTCTTGTACAGACCTTGCCTCACTGTATAATTAGACCTACTTAAACGTTTCAT
    CTTTTGGTATCCTTGCTGCTCAAACATCATGTTATTTAATTTCTCATGACAGTATGTTCATGTTGTCTTC
    TATTCAACGGAGATCACCCAACAGGCTCTCAATGGCTTCCCATACATTCTTTTTCTCATAGGAATATTTA
    GCAGACACTATAAGCATAGCTTTTGTTTGTAAAATATGTCACCACTTTTCTGTTCTTTGGAGAAACACCA
    CATTAAGGCCTAAACACAGTTGCCTTGAGGCATGATAACAGTTTCAAAGCTTGAAGGACAGCGATAGATC
    ATTTCAATAGTGTAAAGGAAAGGTTGATTGGGAGAGAAGTTTCTGTCCAGAGTAGAGGCTCCCAGGGAAA
    GCCATGTTCTGGAGCAGGCAGTGGGGGACAGAGCCTGGCTGGGAAAGTGAGAGGCGTCACCCAAGGCTGA
    ACTAACTAGTTAGAGTGGTCTTAAAATACATTCATGTAAGCTAAGGACTAGATTCTGTAGCTGGGGGAGA
    CTGTTGAGGGATTGGGAAGAGGTCTGAGAGAAACAGCTGCCTCCTGAGTGGGGCAGTGACGTGGCTGGAC
    TGACGCCATGTGGCATTAGAGCGTTCTCAGTCATCATAGGAAGGGACAGCTGGGATGCAACATGGCTCTT
    CATGAGAGCAAGTCTGGAAAGTGCCCTTGTGCTAGGATGGACTTGGCCAGCACTTAAAACACTTCAGAAT
    GGAAGGTTGTGACAGATCGCCAACATTTCAGAGATCTTCTGACCTGTGTTTCCCAAAGGACACAGTGGGG
    ACTGTCTATGATCACACAGCAATGAAAGGTCTTGTCTAAGGCCACAGGTTATTTTTAAAAATCCTAACTA
    ACTTTTTGTTTATATCACTTATTTCTTATTATCTTAATCTCCCCAGGAACATGCGAAACTGTTCTTTGGC
    AGAGAAGGAAGAGATGCTAATTCTGAAGGTCATGCTCTCTAGATCCAGAGTTCGATTTAAGTGGTCAGAT
    TAAGCTCCATCTCTCTCAAGGGGGGACTAAATGTATGTTGAAGACCATACTGTCTTCTTAGCATAAAACA
    CCATGATTGCCATCAGCAAGCCTTGGTGTGTGGCTGTGGTAAATGAAACCTGTGTGGGAGCCAGGTCAGT
    GTGTGGGTTGCCTTATCTATGTCACTGAAAGAGGCAAAATAATCCCATCACAAAATAGCCTGGGAGCAAG
    GCAGTCCCATATCCCTTCCCGTTTGGGTTTTTTTTTCCCATGCCACAGTCCCTCCACAGGTTAGGAGCCA
    AGCTCAGAGTCTCACCTTCATCTTTTGGGGTCCGTCAACTTCAAAGAACAGGTCATTCTCATCACTGTAA
    TGAAAACAGATGTGGTCAAGTGATAGCTCCTTATCAATACTGAGCAGACAGACTTCCATTGTTCATAAAA
    CCACACCTACCATGACTCTTAAAGCTTCCCAAAGACTTCTGGGAAGTTCTACAGTGCTACGTAAGTAACA
    GTCTAGAAGCAGATTCTGGGCCAGGTAAGTGGGTGCTCTGGTTGCTCTCTGTTGACTTCTGACACCTAAA
    TTCTAGACTAGCAGAGTTAGCTAAAGTCTGAGCTCTCTGCCTTTGCTTCCAAGCCAGACTTTGCAAAAGA
    GTCTCTTATCCTGTCCATGTGGAAGTTTTTCTTACCATTGCATCTGTAGGAGTCCCTGTAGTGACAGCAC
    CTAAGTCCCTAGGTTGGGCTTGGGAGTGAAGAGGTCAGTCTTTAAGCTAGGTTGCTTGAACTCTGATAGC
    CACTGAAGGAGATGAGTTGGGGACTCTCTGGCCTCTGAAAGTAGATAGCCCTCCATTCCTGAAAGCAGAG
    TGCTGGATATGTGACCACTCTCCAGTACCCACTGACCTGTCAAAAGGTGGCATTTCACAGTTGAGTTCAG
    GAACAGTTGCCATAGCTGCTTCAGACACCTGTGTGAAGAAAGAAAGTGGGTGGGCATCCAGCGTTAGCTC
    CCCGAGGGGCGTCCTTCATATGTGTTTCTCTTAGCTCTAGTTCAGGGCAGGCATGCTAAACTGGTCTCTG
    AGGAATGAAGGAACGGAGGAGCCGTTGTATAGGTCTAGAACAGTGGCCCTTTTGGAGTCAAGTGGCCCTT
    TCACAGGGGTCACCTAAGACCATTGGATAACACAGATATTTACACTTCATAACAGTAGCAAAATTATAGT
    TATGAAGTAGCAATGAAAATAATTTTAGGGTGTGTGGGGGGTCACTACAACATGGGGAACAGCATTAGGA
    AGATGCTCTGGAAGGAAGCAACAGCAGAGCCAAACCCTAATAAAGAAGCTTTCCTACAAGACAGGAGCTG
    CCTTTTAGATGTCAGCGGCTATACAGACAGTACTCATTTCCAGAGTACTTCACAAACCATGGAGGTACAG
    CTACTTTTTATAAATAAAAAGCCCAAAGTCCATCAGTGGGGGGGGGGGAAAGAGAGAGAGAGAGAGAGAC
    AGAGAGAGACAGAGAGAGAGACAGAGAGACAGAGAGAGACAGACAGAGAGAGACAGAGACAGACAGAGAG
    AGAGAGAGATAGACAGACAGAGACAGAGAGAGAGACAGACAGAGACAGAGAGAGAGAGACAGAGAGAGAG
    AGATAGAGAGAGACAGAGAGAGAGAGAGACAGAGAGAGAGAGAGACTTACTTGCACAAGGAAGCTTGGCT
    GGAGAGGATCCCAGATGAGCCTATTAGGCCTC
  • Protein Sequence : Show Sequence
    >gi|6680415|ref|NP_032387.1| interleukin-1 beta precursor [Mus musculus]
    MATVPELNCEMPPFDSDENDLFFEVDGPQKMKGCFQTFDLGCPDESIQLQISQQHINKSFRQAVSLIVAV
    EKLWQLPVSFPWTFQDEDMSTFFSFIFEEEPILCDSWDDDDNLLVCDVPIRQLHYRLRDEQQKSLVLSDP
    YELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKK
    KMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis galU mutant vaccine , Francisella tularensis guaB mutant vaccine
10. IL-6
  • Gene Name : IL-6
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16193
  • NCBI Protein GI : 13624311
  • Genbank Accession : AC112933
  • Protein Accession : NP_112445
  • Taxonomy ID : 10090
  • Chromosome No : 5
  • Gene Starting Position : 30013160
  • Gene Ending Position : 30019967
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 6
  • Protein pI : 7.5
  • Protein Weight : 23291.86
  • Protein Length : 211
  • Protein Note : Also known as Il-6
  • DNA Sequence : Show Sequence
    >gi|372099105:30013160-30019967 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
    ACCAAGAACGATAGTCAATTCCAGAAACCGCTATGAAGTTCCTCTCTGCAAGTAAGTGAAGGCAGTTCCT
    TGCCCTCTGGCGGAGCTATTGAGACTGTGAGAGAGGAGTGTGAGGCAGAGAGCCAGCATTGTGGGTTGGC
    CAGCAGCCATCAGCTAGCAGCAGGCGCCCAACTGTGCTATCTGCTCACTTGCCGGTTTTCCCTTTTCTCC
    ACGCAGGAGACTTCCATCCAGTTGCCTTCTTGGGACTGATGCTGGTGACAACCACGGCCTTCCCTACTTC
    ACAAGTCCGGAGAGGAGACTTCACAGAGGATACCACTCCCAACAGACCTGTCTATACCACTTCACAAGTC
    GGAGGCTTAATTACACATGTTCTCTGGGAAATCGTGGAAATGAGAAAAGAGGTGGGTAGGCTGTGAAACT
    GATGAAGACCCAGTGTGGGCGTCCATTCATTCTCTTTGCTCTTGAATTAGAAATTCTCTGCTGGGATCTA
    GGGCCCTTAGGATTTGAAGCTAAAGGTCAGACTAGACTGTGTTCTCTCTTTCTCACCTCTTTGCTGGTTT
    TGAGTGGAGGTTGGGAAGGGGTTCCTTTCCTGTCTGGAAGATACAGAATGTGACTGCATTTCTAGAAAAT
    TCACAGTGGGCCATTCTCTGTTCCAATTTAGCTATTGCTTCTTAGGTGGGGATTCCTTTTCCCTCATTAT
    TCCTTTTTCCTTATCTCTTTGCCTTCATTTATCCCTTGAAGTCTACAAGGACCAGAAAGTGCTTTTTGGC
    TGAATGTAGTAAGTTCTGTGTGTGTGAAATCAGGATGCTCTAGGGTCAGCCCAGATAAGATGCATATTGT
    ATAGAGCCCAATAAAGTGCTAACACACACACACACACACACACACACACACAACCTAGATTAGGCTGGGC
    ATGGTGGTGGATGCCTCCCAGCACTTGGGAGGCACACGCAGGAAGATCTCTGTGAGTTAGAGGCCAGCTT
    GGTCTACATATTGAGTTCCAGGATATCCAAGACTACATTGAGAGATCCTTTCTCAAAAAAAAAAAAAAAA
    AAAAAAAAACAACAACAACAAACAAACAAACAAACAAGAAAAAAAAAACCCAACCAACCCTACCTAGATT
    AAAATATCTCCCATCTTTAAATGAAAAGTATTTTGACTCTGCTTGACAGAAAGGAAAAAAAAAATCACAA
    ACAAGATTAATAGCCTAGGTCTGGGTGTGTAGAACCAACTTTGTAATCTTGGGAAAATCCTCAACTATTT
    AGTACTCTTTATCTGCATAATTATGGAGTTACAGATAATGGCACAGACCCTTCCAGATGGAATATTCTGT
    GACTTAAATTTATTTGCCCCAAGATATCATCAAAATTTCTGTCCTTTACCAAGTTCCTACTCTATGCTGG
    GCAGTTTTTATCGTTGAATCCTTGAGACAAATGTATAAGAGGGACATCAATAATTCTTTATTGAAACATA
    GGAAATACTTAGGAGAGTAACAAGCTTAAAGCCAAGGGACTGAGCCTAAGGGTGCATTTTTATTTATTGG
    GCTGCTTAGTAGGGTTCTAAGAAGGTATAGCTTCCCTTTTCTGTGTCCTGGCTATGAAAGAGCATGACTT
    GGCCCTCTAGTGGTGCTTGTTGTAAGAGGTGTTTAAGTGATTGCTCTGGTAATCTTTTGCAGTTGTGCAA
    TGGCAATTCTGATTGTATGAACAACGATGATGCACTTGCAGAAAACAATCTGAAACTTCCAGAGATACAA
    AGAAATGATGGATGCTACCAAACTGGATATAATCAGGTAGAAACTTGTCACGTTTACTTTCAACAATTCT
    TCAAGGTTCTCTCTTGCACGTGATACCTGCATTTGTACAGTCCCAGTCAGGCAACAAAGATGGGGGGATA
    CATCCAATACCACTTGGATGTAACTGGCCTGCAGTAGCTTGAATACAAAAGGAGATTGAGAGGTACCTCA
    GCAGCTGCTAGGGTGAGGGAAAGTTTACCAACCAGTGCTATAAATGTTCACTCAATCTATGTCCATCAGG
    AGCTAAATGTTTAAAGTCTCTATTCTCATCTTGGCTTTGAGGTGTTAGATGATTAAAAAAAAAACATTTT
    TTTTGTCTAAAAAAGTGGAAGAGTAGATATAAAACATGTTATGTAAACGTCATGAGGAAAGTAACTTACA
    GATTTAATTAAAAAGCAGTTTATTTAAACAGGTAAAGGCCCACTATGCTGGGATTCTTCACCACTGTTAA
    ATGTTAGAAGTCATAGCAAGTTTAGCTAGTTCATGAGCCATCTCTTCTCTGGCTGCCCCTGGAAGGATCC
    AGGCCTACACTCCCTACTGAGAACACTCTTCACACCCCTCTCCTTCCAGCCGGTGAGATAGTCTACATCA
    GGTCTCAGGACAAGTCAGACAAGAACAAGAGTACAGGAAGGGCACTGCAGGATAGAAGGGAAGGAGTGAG
    GGTCAGAAGTGGATTCCCACTGCCACACTCTGGGGTAGGGGGTGGGGGGATATTAAGTGGTGCCTCTCTC
    TACTTGTCCAGATAATTCAGGAGGTCAGCTGATGCTGCCTGTTGTCTAATTCTGTAGAAGATACTGAACA
    GCAGGAATCTTTTTTTCAATTTTTTATTAGATATTTTCTTCATTTACATTTCAAATGCTATCCCCACAGT
    CCCCTATACCCTCCCCCCCCTCCCCGCTCCCTTACCCACCAACTCCCACTTCTTGGCCCTGGCATTCCCC
    TGTACTGGGGCATATAAAGTTTGCAAGACCAAGGGGCCTCTATTCCCAATGATGGCTGACTAGGCCATCT
    TCTGCTACATATGCAGCTAGAGATATGAGCTCTGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTAT
    AGGGTTGCAGACCCCTTCAGCTCCTTGGGTACTTTCTCTAGCTCCTCCATTGGGGGCCCTGTGTTCCATC
    CAATAGAAGACTGTGAGCATCCACTTTTTGCCAGGCATTGGCCTAGCCTCACACGAGACAGCTATATCAG
    GGTCCTTTCAGCAAAATCTTGCTGGTATATGCAATACTGTCTGGGTTTGGTGGCTGATTATGGGATGGAT
    CCCTAGGTGGGGTAGTCTCTGGATCTTCCATCCTTTTGTCTTAGCTCCAAACTTTGTCTCTGTAACTCCT
    TCCATGGGTATTTTGTTCCCCATTCTAAGGAGGAATGAAGTATCCACATGTTGGTCTTCCTTCTTCTTGA
    TTTTCTTGTGTTTTGGAAATTGTACCTTGGGTATTCTAAGTTTCTGGGCTAATATCCACTTATCAGTGAG
    TGCATATCAAGTGACTTCTTTTGTGATTGGGTTACCTCACTAAGGATCATACTCTCCAGATACATCCATT
    TGACCAAGAATTTCATAAATCCATTGTTTTTAATAGCTGAATAGTACTCCATTTGTAAATGTACCACATT
    TTCTGTATCCATTCCTCTGTTGAGGGACATCTGGGTTCTTTCCAGCTTCTGGCTATTATGAATAAGGCTG
    CTATGAACATAGTGGAGCATATGTCCTTATTACCAGTTGGAACATCTTCTGCGTATATGCCCAGAAGAGG
    TATTGCTGGATCTTCCGGTAGTACTATGTCTAATTTTTTGAGGAACAGCCAGACTGATTTCCAGAGTGGT
    TATACAAGCTTGCAATCCCAACAGCAATGGAGGAGTGTTCCTCTTTCTCCACATCCTTACCAGCATCTGC
    TGTCACCTGAATTTTTGATCTTAGCCATTCTGACTGGTGTGAGGTGGAATCTCAGGGTTGTTTTGATTTG
    CATTTCCCTGATGATTAAGGATGTTGAACATTTTTTCAAGTGCTTCTCAGCCATTCAGTATTCCTCAGTT
    GAGAATTCTTTGTTTAGCTTTGTACCCCATTTTTAATGGGGTTATTTGAATTTCTGGAGTTCAGCTTCTT
    GAGCTCTTTGTATATATTGGATATTAGTCCCCTATCAGATTTAGGATTGGTAAAAATCCTTTCCCAATCT
    GTTGGTGGCCTTTTTGTCTTATTGACAGTGTCTTTTCCCTTACAGAAGCTTTGCAATTTTATGAGGTCCC
    ATTTGTCGATTCTCGATTTTACTGTACAAGCCATTGCTGTTCTGTTCAGGAATTTTTCCCCTGTTCCCAT
    ATCTACGAGGGTTTTTTTCCCACTTTCTCCTCTATAAATTTCAGTGTCTCTGGTTTTATGTGGAGTTCTT
    TGATCCACTTAGACTTGAGCTTTGTACAAGGAGATAAGAATGGATCAATTCGGGCTGGAGAGATGGCTCA
    GTGGTTAAGAGCACTGACTGCTCTTTCAGAAGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGTTCAT
    AACCATCTATAATAAGATCTGATGTCCTTTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATGTA
    ATAAATAAATAAAACTAGATTATTTAAAAAAAAAAAAAAAGAATGGATCAATTCACACTCTTCTACATGA
    TAACCACCAGTTGAGCCCGCACCAATTGTTGAAAATGCTCTCTTTTTCCCACTGGATGGTTTTAGCTCCC
    TTGTCAAAGATCAAGTGACCATAAGTGTGTGGGTTCATTTCTGTGTCTTCAATTGTGTTCCATTGATCTA
    CCTGTCTGTTGCTGTATCAATACCATGCAGTTTTTTTTATCACAATTGCTCTGTAGTACAGCTTGAGGTC
    AGGCATGGTGAGTCCACCAGAGGTTCTTTTATTGTTGAGAATAGTTTTTGCTATCCTAGTTTTTTTTTTT
    TTTTTTTTTTGAACAGCAGGAATCTTATTCTCAAATTGAATCTATTCCTAGAAAGAACTGACTTCCTTTT
    CCATTTTACTTATAGGAAATTTGCCTATTGAAAATTTCCTCTGGTCTTCTGGAGTACCATAGCTACCTGG
    AGTACATGAAGAACAACTTAAAAGATAACAAGAAAGACAAAGCCAGAGTCCTTCAGAGAGATACAGAAAC
    TCTAATTCATATCTTCAACCAAGAGGTGAGTGCTTCCCCATCTCTCATGCAGTGTGGGAAAGAGGACACC
    CGGCACCTCAGGGTAGCGGCACTTTTTCCAGACAGCTGCTCAGAAGGGAGAGAGTCTGAACAACAGTTCT
    TGATTATAAGGCCTTGCTTTGTTTTACTTTGGGGTTTTGTTTGAGGTTCTCTTTTGCAAAGAACATCAAT
    ACCTGCTTTAAACTGTATTAATAGAATGTTACTAATTGCGTAAGAGGTATGAAAACTATGACAGCCATAC
    ATAGTCACCCATTATGAGAGCACAGAGACAAAAGTGACTTTAATATTTAATCCTTGGCAAGTGACATTTT
    TGTAACCAGAGTTCTAATGCAGAGAAGTAGCCAAAAGCTAAAATGTCAGGGAAGGAAGGGATCAAGGGCT
    TCTGGCTACCATTAGCCAGAAGAATGGTAGATATGGAGGATTAATGACTCAGAGTGTGGGCGAACAAAGC
    CAGATGCAATAAGAAGGGCCTGGAATGAAACCCTCTTGCTAAGGCTGCTTTTGCCACTTGTAGTTTCTTG
    CCTTAAACCAGAGAGTTTCCCAATTTAATGTGCACAGGAACCACTTAGGGTCTTGTTCCAGCAGGGTCTT
    ACTTAGGAGGTCTAGGAAGGGAACTAAGATTCATTTTTGTAAACAGCTGCTTGTTTATGACCCTGCTATA
    TAGTATTCAGGCTCCTAAGTAGTGGGGCAAGCCTTCCAGTTAGTCTTCCCCATGCCAGTGGGCCCATGGA
    AGGGTGTTTCCAGACTTCTTCATGCTACCACACTAAGAGACTCTCAACAGAGTCTGAATGGAAACCACGA
    AGGAACACATTTGTTTTAGATTCCTCTGTGCCACCTTTACTGATGGGAGCTTCTGTTTTCCAGTAGAATA
    CAGTATGTTAGCATGGATGCTTGGATAACAGACAAATAAGATGGTGGTGCTGACCTCTGGACGCTTACTC
    TCTAGTGGCAGACAGAACAGTAAGGTTAGAATTCTGTTGCTATTAAAAAACTAATAATAATCACCTTGAA
    AAAGAATGGAGTTGTTAGGCATGGGTCTCTCTCGAGTAAGCTTGGAACAAAAGCATCTCCCCTGGCTTGG
    GTGAGTCAAAGCAGATGGACTTAGCTCGTCTCATTCATTCTAAATTAGAACTTCTTCCCACAGCCCAGAA
    CACGCCACAAGAAAAAAAAAATGTGCAATATTTAACCAGTCTTTGTTTTTTCCTCCTTTAGGTAAAAGAT
    TTACATAAAATAGTCCTTCCTACCCCAATTTCCAATGCTCTCCTAACAGATAAGCTGGAGTCACAGAAGG
    AGTGGCTAAGGACCAAGACCATCCAATTCATCTTGAAATCACTTGAAGAATTTCTAAAAGTCACTTTGAG
    ATCTACTCGGCAAACCTAGTGCGTTATGCCTAAGCATATCAGTTTGTGGACATTCCTCACTGTGGTCAGA
    AAATATATCCTGTTGTCAGGTATCTGACTTATGTTGTTCTCTACGAAGAACTGACAATATGAATGTTGGG
    ACACTATTTTAATTATTTTTAATTTATTGATAATTTAAATAAGTAAACTTTAAGTTAATTTATGATTGAT
    ATTTATTATTTTTATGAAGTGTCACTTGAAATGTTATATGTTATAGTTTTGAAATGATAACCTAAAAATC
    TATTTGATATAAATATTCTGTTACCTAGCCAGATGGTTTCTTGGAATGTATAAGTTTACCTCAATGAATT
    GCTAATTTAAATATGTTTTTAAAGAAATCTTTGTGATGTATTTTTATAATGTTTAGACTGTCTTCAAACA
    AATAAATTATATTATATT
  • Protein Sequence : Show Sequence
    >gi|13624311|ref|NP_112445.1| interleukin-6 precursor [Mus musculus]
    MKFLSARDFHPVAFLGLMLVTTTAFPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELC
    NGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKAR
    VLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQ
    T
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis FTL0552 mutant vaccine
11. Il12a
  • Gene Name : Il12a
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16159
  • NCBI Protein GI : 226874945
  • Genbank Accession : AC121306
  • Protein Accession : NP_001152896
  • Taxonomy ID : 10090
  • Chromosome No : 3
  • Gene Starting Position : 68690643
  • Gene Ending Position : 68698546
  • Gene Strand (Orientation) : +
  • Protein Name : transcript variant 1
  • Protein pI : 8.33
  • Protein Weight : 25111.9
  • Protein Length : 236
  • Protein Note : Also known as p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232
  • DNA Sequence : Show Sequence
    >gi|372099107:68690643-68698546 Mus musculus strain C57BL/6J chromosome 3, GRCm38 C57BL/6J
    CTGCCACCTACTCCCTTGGATCTGAGCTGGACCCTTGCATCTGGCGTCTACACTGCTGCTGAAATCTTCT
    CACCGTGCACATCCAAGGATATCTCTATGGTAAAAGTCCAGAAAGGCTAAAGATGTTGTCCACAATCTCT
    CGGCAATGACCAGATATATCCCCACCAGTATCACAACTTGCAACGCGCTCCGTTCCCATCTGCCCTCTGC
    CTCCCTACTGTGTTGATTCCCATTTCCAAAACTAGCTCAGAATGCATCCCACTGGCAGCCAGGCAATGCC
    TTCTTGCAGATCTGGAGGCTTCAGGAGCCTTTGCTGCCTCTTAGGCTCCTGCAGAGAGATGGCCTTGCTC
    TCTCTGGGCCCTTTTCCCCCAAGATGCTGTGCAGTCGTCTCACTAAATCAAAACTGCCAGAACTGAGTTT
    TAAGCGATATATAGCCACCACATCTATTTTGTTCCTTTATACTTTCAAAGTAGGACAGATTCGCAAAATA
    CATTGAGTCAGACTCAGTGTCCACGATGGAGAGACGAGTGGGTAGCTTGTGCTTCTTTAGAAGGGGCACT
    GGGCAGCGTGTTAGGGCTAGGGGGTAGTGGGGCAGGACAGACCTGTCCCGGGACAAGAGTGGCTACTCGC
    AGGCCGGTTTTGCTTTCGCTCTGAGTGTGGTGGGCGGTGGGACGGTCCCGAATCTCCTCAAACGGGCACT
    CCGAGAGTTAATTCGAAAGCGCCACCAGCCTTGGGGGCGATCGACGCACTTGTCCTTGAGATGTAGAGAC
    AGAGAGAGAAAGCAAGAGACACAGTCCTGGGAAAGTCCTGCCGGCTATCCAGACAATTATAAAAATGTGT
    CTCCCAAGGTCAGCGTTCCAACAGCCTCACCCTCGGCATCCAGCAGCTCCTCTCAGTGCCGGTCCAGCAT
    GTGTCAATCACGCTGTAAGTACCTGAGGGCCGGGACCAGGGTGACTTGATGAGAGGAGAAGGCTTGAGAA
    AGAACAGACAGAAGCCCTGGACCCATTAAGGGAGACTAAAGGGCTGCCAGCAACTGCAGGCCAAGGGAAG
    GAAGAGGGGAAAGATGGTCGTGCTAGATTGCGCTGCACCGAGCATGGTCTCATTATAGAACAACCCTGGG
    GGCTACGGCTATGCCCGTTTCACAGAAGAGAACTAAAGCCCAGCCTTGAAACCAATGTAAGGAAAATGCA
    GGTCTCAGGCAGTCCCCAAACAAGATCTGGGAGGTCTTGCTTCATCTGTCAGCCCCGGGGAAGGGCTTAA
    ATTATGTGTGGAGGGAGGTCAGTACCCAGAATGGATGGAAAAAATTGCCAGAATGGCTGAAGAAAGAGGC
    AGGTCAAAACCAAGTTGACAAGCTCCACCTGCCCTTTGTTTCCCTTCCAGACCTCCTCTTTTTGGCCACC
    CTTGCCCTCCTAAACCACCTCAGTTTGGCCAGGGTCATTCCAGTCTCTGGACCTGCCAGGTGTCTTAGCC
    AGTCCCGAAACCTGCTGAAGACCACAGATGACATGGTGAAGACGGTGAGTTTCTCCTGTCCTTCTGGCTT
    CAAAGTTCCGCTGTTGACATGACTTCTAACCTATCTGGTATCTGCTGGGAAGTTTATGGTATCTGGCAGG
    AACCGTAGAGGGTTTCAAGACTTCTAAATATAATCTAAAGGAAACTGTGTCTCTAGAGAAAGGAAGAATG
    GTAATAAATTGTAACGGCATCGGTTTAGGCTGTGTCTACACAGAGGAAAGTGGGGACTTGCAATGTCCCT
    CCCAGCCTGCCTGCCTGTGTGACACAGTGTCAGGTGCTCCCTGCTTCTGCTTCAGGGCAGGTGGAACAGG
    GAGACAGTCTCTAGGTACACTCTCTGTGCTTCACAAGCCATAGAGATGAATTAGATTGTCTCTCCCCCTG
    CTCACCAGTGTCCAGCACTGTGATGCAGTCCCTTGTCGGATGGACACATGTCAGGGAGGACTCAGACATC
    AGATAGCAAGCAGGTCCAGCCTCCAGTTTTCTGGGGCAAGTGTGATCCAGTTTTAGTCACGGCTCCACTC
    ATTTTTCTCAGGTTTCATGTTTGAATGCAAAATAGCCTTTTCCGTTGTAAAATTGCCACGGTTTCCTCTC
    GCTTTGGGGACAAAGCTACAGCAAAATGCTAATGTGTTTGAGATATCCTGCAGCTGCTAGGGCTGGAGAT
    CCAGTAGAATTCTGGCTTAATTATGAATGGGTCTCCCGGGTTCTATCCCCCAACAGAGCGTGCGTTCATA
    AGCACCTGGGAACAGAGGAGGGAAGGAGGGAAACTCTGTCTTCCTGTTTTGTTTTTAAATCTTTCTTGAG
    GATCTTCTAGCTTGTTGTCTTATGGCTATCCCAACATAAGCTCCGCTCCACAAGCCCTTCTTGGTGAATT
    TTCTTTCTTTCCTTCCCGTCTTGCATAACGTGCTGCTGGCTGTTCCTCCAAGCTCAGTCTTAATTGAGTG
    GGGTTCCCAGGAGGGAGAGATGGCTCTGTGGTTAAGAGAGCTGGCTGCTCTTCCAGAGGTCCAGAGTTTC
    CACACCCTCATGGTGGCTCACAATCATGGTAACTCTGATCATAGGGAACCTGTCGTTTTCTCTGTGCCTC
    TGTGGGCACTGAGCACATGCCACACAGACATCCATGCAGGCAGAACACTCTTGGCCATGAAACCTTAAAA
    TGAAGGAGAAACCTTACGAGAGGTTCCCTAAGGAATCCCTGCAGAAGGGAGAGATTGTTTTCCTAAGATG
    AGGTTTGGTGTGGAGGGGATGAGGTATTCAAAGTTAACTAGCCGCTCCTCACTCCTCTGCTGCCAAATAC
    CAGCACTGGGCTTCGAGACACAATAGAATTCGACCTTGTTTTCGTTTGGGTCACTATTGCTCTCTAGTTG
    AACACGGAATCCACGGGGGCACATTGCTGTCTGTCCTTTGTCACACCCCAGGACCACAGCTGCATGGAAA
    AGTCATCGCAGGCTTCAGTCAGAGAGCTGTTGACTTCCTAATATGTTGTGTAATTCTGTTCAGTGGTGCT
    TTCCAGCACACCAGGGAATAAGAGACAGTCCCCGCGTATGTAAATTAAAGCTCAACTAGTATTTAGGGCT
    TGCAAGTTTGTTACAGCCATCAAGCTGTGACTTTAACAGTTTCCTCTTTAGAAGTTAAACTTGAAAAAAA
    ATAGAGGGAGAGAGAGATTCACAGGGCACAGCTGCTTGGAAAAAGGAAAAGCCACAGGACTCACCAGAAG
    CAAGCAGCACAGTGTGGCACTGATGCTGATGAGTACAGCCTGCTGCACCCCAGTGTTCACATGGTGAAAA
    AAATACCCTGCTGGCGGACAGCCTAGGCAAAGGGGGAGGAGAGTAGGGAGAGCGTGCGGGAGGTTGGGTT
    TCTTAGCTTATGAAGAGCCTGAGTTTCCTACAGGCCAAAGAGATGACGGTTCAGAGTGGCCAGCCTAGGC
    TCTGCTGATCCACTGCGATGCAGGTCTCAGAGGGAACCAGCTGTCTTGCTGAGAGATTCACCTCAGCTTC
    TCCTTATGTTTTTCCCAGGCCAGAGAAAAACTGAAACATTATTCCTGCACTGCTGAAGACATCGATCATG
    AAGACATCACACGGGACCAAACCAGCACATTGAAGACCTGTTTACCACTGGAACTACACAAGGTATGGAG
    GACCCCCACCCAAAAAACAACAACAACAACAACAAAAAACACAATTAAGAGCCGTGCAGCCAACCTCAGC
    TGTCTCCAGATACTTACTTGTAGCTATCAAACGTGAGAGTTTTGAAGAAAAAAAAAAAAGTCAACAACCA
    TACAAATACTTCTACAAGCTGAAGGATATACTCGGTAACTAGGAGGTGGCTATGGTGGTATAGACCTTTA
    ATTCCAGCACTTGATGGATAGAGGAAGGCCGGCTCCTGTTAAGTTAGAGGCCAGTCAGGGATACACAGCA
    AGATTTCATCTTTAAAAAGAAAGGGGGTGGTGTGGCGGGTGAAGCTTACTTATTGAAGTGCTCATGTCAC
    AAGCCTGGGGACCTAAGTTCCCATCCCCAGCACCCATGTAGGAGCTGGGTGTGATGGTTTCACATGCAGA
    GACAGGAGGATACCTGGGCCAGCTAGTCTAGCCGAATCCATGAGCTGCAGGCCCAGTGAGAGAACCTGTA
    TCAGAAAACAAGAAAGTCAGGAGAGCGGCTGAGAAAGACCCCAAAGATCATCCTCCGATTTCCACGAGTA
    CATGCACCTGCACACACGTGCACACAGGAATCCTATACATAGCCACCCAAAAGCAGTTATGATGGTGAAG
    TTTCTTTTACTGGAATGAAAAGTTAGTTTGAGAAAAAAATATCAGAGAGATTTATATTAAGAGTGAATGG
    GTGGGGGGTATGCATGCCTTTAATCTCAGCACTTGATAGGCAGAGGCAGATGGATCTCTGTGAGTTCAAG
    GCCGGCCTGGTCTACAGAGTAAGTTCCAGGACAGCCAGTGCTACAATAGAAACAGGTGATGGTGATGATG
    GTGATGATGGCGGTGATGATGATGATGATGACGAAGACAAACTCCTCGGTTTATGATTTTCTTCTAGAAC
    GAGAGTTGCCTGGCTACTAGAGAGACTTCTTCCACAACAGTAAGTAAGCACTCTAAGGGTTCCTTCCCCA
    TGACGGATTCATAACACTGATGCCTGGTCATTCTTTCTCTAGAGAGGGAGCTGCCTGCCCCCACAGAAGA
    CGTCTTTGATGATGGTAAGCCACATCACTTTTCTGAAATGCAGCAGGGTCCTGTAGTTCCAGCCCATCTC
    AATGATTCCTTGCCCATTGTGTCCTGCGCTCTAGACCCTGTGCCTTGGTAGCATCTATGAGGACTTGAAG
    ATGTACCAGACAGAGTTCCAGGCCATCAACGCAGCACTTCAGAATCACAACCATCAGCAGATCATTCTAG
    ACAAGGGCATGCTGGTGGCCATCGATGAGCTGATGCAGGTAAGCCTTCCTCCTATCAGCCAGCGCTCCTT
    TTCCATGGTGAAGAGAGGTCCTTTATTAGATATGATAAAATCAGCACAGCTTGGGTTGGGAGAGACCTGA
    ACACCCCCACCCCACCCCACACCCTCATCCCCCAACCATGAAAGGCCTTGCAAGGTTACATTTGCTTAGA
    GAGCCTTGAGGGGGCCTCTACAATAAGGGCAAATGCAAGCAGCCTTCTACTTGTTGACTCATGATACAAA
    TCAGGCACTTTTCAAATGCTTCTTCTCATACCATCCCCACAACAAGCAAATAAGGTCTCTACAGAGGAGG
    AAACTGAAGCTTAAGAGGATGAACGAGGGTTCCCTACAAAAGGGTTTTTTGTTGTTTGGTTTGGTTTGGT
    TTGGTTTGGTTTGGTTTGGTTTTTCCGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTCAC
    TTTGTAGATCAGGCTGGCCTCGAACTCAGAACTCCACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGG
    TGTGCGCCACCATTGCCCAGCTTATTGTTGTTTTAAATACATTCTTTTCTTAAAGGTTCAAGATTAGGAC
    TGGAGAGACAGTCCGATGATTAAGAGGACTTGAGTTTGGCCTGGGGCTCCTATGTAACAAACAGGGTGTG
    GTCCTGAACTGCTCTCTAACTCCAGAGAGTGGAGACAGGAGGCCTATGGAGGCTTTCTTGCTGCAAGTTT
    AGCCATGAAATCTCCAGGTTCAGGGAGAAAGCATGCCTCAAAGAAATAAGATGGAGAGTCACGTGATCTC
    TTCTCTGGCTTCTGCATGGTGTAGGCACAGATATGCACATGTTTGAGTGCATACAATACAGGCAAACATA
    CACGTGTCCTTTTTAAAATGTATAGGATGGACATTCTAGGGGCTGCACACATATTCAAATATTACTAGGG
    GAAGCACTTGGGGCTGGTGTCCAAGTATAATCTATTTTGAACCAAAATGTGACCAAAATGAAAAGTGGTC
    TTACATCTAGGTAATTACAGTGTTAGGTATCCGTGGGAACTTTGCTGTACCACCAGTAACACAAAGCACA
    GGAGTGTGCACACCAGAGACAAGCACCTTGGCAACCAAGGCGTGGGATGAACATATGTTCAGAAGTCACT
    GCCTGGAAAGCAATTGTTTTTCATTTCATACATACTGTCTTGCACAGGGTTTCTATGTCTTAGATAAAAT
    ATCATGACGGAAAGCAAGTTGGGGAGGAAAGGGTTTATTTCATCTTGTAGAGTGTAGTGAGTCTATTACC
    CAGGGAAGTGGAGGCAGAAGCTGATGCTGGAGAGGACTCCTGGTGACTGGAGTGCTCCACATGACTTGCT
    CAGTGTGCTTTGTATAGCACCCAGGACCACCAGGCTAGGGTGAGCTGGTGCCTCCCACATCAACTGTCAA
    TCAAGAAAATCACCAGAGGCTTGCCCACTGGCCAATCTGGCTGGAGGTGTTTTTCTCAATGACCACAGGC
    TTGTGTAGAGTTAACATAAAAGCACCCAGGACATTACATATTGCCAGATGACTCCTTTGAAAGACAGTGA
    AGTTTATGCAGGTCAAACAACCTATCATGTAGAGATTTGTAATGGATATTGCATGAACAAGGCCAGAAGT
    CAGGTGATTTGGATAGTAGGAAGAGGTCTACCAGGGACCAGCCAACTATGCTGACAGGGTTAACTCTTGT
    CAGCCTGTTTGTAAAATAAGATGACTTTAAATAGCCTATGTCTCTCTTGAGATTTTTGTGAGGAGCAAGC
    AAAAATCTTAAACATCACAGAAACCTAAGTTCTTATTCTATGTTATGAAATCAAAGGATCGAGTGGGGAA
    ACACGGTACTGGCTTGTTTATCTACCACAAATAGCGTTTCTATCAGAGATAGGTCTGTTGTTGATAATAA
    GCTCTAATCCATAGATCCTACTTACCCTCCAACTCCCTCCTCTAAAAGAACAGGACTTCTTAACATAATT
    TATAAAAATTAATTAGCTATTGCTGCAGGTGCTACCTCATAGACTCACACCTTCTGAGAGATCAGCTCTA
    ATGACAATTTCTTTCTGGAAAATTTAGGGGTCTTGTCAGAGATCATTAAAAACATAGGTATATGCTCACA
    AAAGAGTATTTCTTCCCTCTTTTCCTGCAGGAACACAGCAGGAGTAGATGTGAAATGGGTCTAGGAGTGT
    GATGAGGGGCTAGGTTGCAGGCCCTGGACTTTGCATTGACTGTCTCCCATTTTGCAGACAAACATTCTAA
    TTTTTAAATTGTGCATATGCTATGCTTGGTGTTTCTTTCTAGTCTCTGAATCATAATGGCGAGACTCTGC
    GCCAGAAACCTCCTGTGGGAGAAGCAGACCCTTACAGAGTGAAAATGAAGCTCTGCATCCTGCTTCACGC
    CTTCAGCACCCGCGTCGTGACCATCAACAGGGTGATGGGCTATCTGAGCTCCGCCTGAAAGGCTCAAGGC
    CCTCTGCCACAGCGCCCTCCTCACACAGATAGGAAACAAAGAAAGATTCATAAGAGTCAGGTGGTCTTGG
    CCTGGTGGGCCTTAAGCTCCTTCAGGAATCTGTTCTCCCATCACATCTCATCTCCCCAAAGGTGGCACAG
    CTACCTCAGCATGGTCCCCTCCATCGCTTCTCTCATATTCACTATACAAGTTGTTTGTAAGTTTTCATCA
    AAATATTGTTAAGGGGCGAAGACGTCCTCCCCTCAATGTGTTAGCAGAAGAGCAAGAACTGATAAGCTAT
    TGTTTTTGTGCCAAAGTGTTTATGAAAACACTCAGTCACCCCTTATTTAAAAATATTTATTGCTATATTT
    TATACTCATGAAAGTACATGAGCCTATTTATATTTATTTATTTTCTATTTATTATAATATTTCTTATCAG
    ATGAATTTGAAACATTTTGAAACATACCTTATTTTGTGGTTCTAATAAAGTAATGTTATCACTT
  • Protein Sequence : Show Sequence
    >gi|226874945|ref|NP_001152896.1| interleukin-12 subunit alpha isoform 1 [Mus musculus]
    MVSVPTASPSASSSSSQCRSSMCQSRYLLFLATLALLNHLSLARVIPVSGPARCLSQSRNLLKTTDDMVK
    TAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLC
    LGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKM
    KLCILLHAFSTRVVTINRVMGYLSSA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis guaB mutant vaccine , KKF24
12. Il12b
  • Gene Name : Il12b
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16160
  • NCBI Protein GI : 6680397
  • Locus Tag : RP23-388G23.1
  • Genbank Accession : AL607030
  • Protein Accession : NP_032378
  • Taxonomy ID : 10090
  • Chromosome No : 11
  • Gene Starting Position : 44400062
  • Gene Ending Position : 44414016
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 12b
  • Protein pI : 6.5
  • Protein Weight : 35952.67
  • Protein Length : 335
  • Protein Note : Also known as p40; Il-12b; Il12p40; Il-12p40
  • DNA Sequence : Show Sequence
    >gi|372099099:44400062-44414016 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
    CAGAAGGAACAGTGGGTGTCCAGGCACATCAGACCAGGCAGCTCGCAGCAAAGCAAGGTAAGTTCTCTCC
    TCTTCCCTGTCGCTAACTCCCTGCATCTAGAGGCTGTCCAGATTCAGACTCCAGGGGACAGGCTACCCCT
    GAACCAGGCAGCGTGGGAGTGGGGTAAGTGGATTCTGGGAGCATCTCGGATGGCTTTCCCCGCTGGTGGA
    AACCAGGGGCTTTGACGACAGGGGCTTCAAATAGTCATTTAAAAAGTCAGCTAATTACAGATGTGATGGC
    GCACACCTGTAATCTCCAAATCTGGGAGGCAGGAAACAGAATGCACAGGAGTTTAAGGCCAGATTCAGCT
    GTGTAATGAGTTTGAGAGAGTCTCAAAAAATTGAGCTACTAATAATATGTGCCAAGCATTGTGGACAGAT
    GTGTATTTGATAGAAAGGTTTGCTTTGTCTATATAGACACATATACTATGTGCATTTTCTTAAGTATTAA
    TGCATATGTGTTAAGGAGTGTTGTTAAAATTCTGTTCTTGCCTTAAGAGTGAAACTTAAAAAAAAAAAAA
    GCCTTTCATGTACCCCTTCAAGCCTAAGCTCATCACTGAATGTCCTACCTGTGATGGCCTTTAGAATCAG
    CTATGATAATCAAAGTGGAGAGCTGGGTTTGGGGGTTCCTTTGGATTTTAACTAACTTTGCCACCTCTTC
    AAAACTGGAAAGCCCAAGGCATGTTCCAAACTATTTCCTAAAGGGTTCTAAAATCTGTGAGAAAGGGAAG
    GCTAATCTTCATGGGTTGGCTTCCTCTGCTTGCCCAGGTGAAATCATGAGCACCAGATGGGGCAGGGTGA
    TGGGGCAGGGTGACATCTTGCTTTCACCCAGGGATCACTGAGCCTCAGCTGGGGGCTCCGTTTGTTCAAG
    CAAGGCATTTCAAGATGAAGTTAGGTCACCCCCAAGAATTCATTTTGCTGAGATCTGAAATACTGTGATT
    ATTGCACCAACAATCCATGGACTCTCTCTAGGCTAGATTCTGGATATAAGACTAGACAGATATGACCTCT
    GTCACACACCTCGGTATGGGAAAGGGGGGACAGACATTAAAGACGTAATCAAATGATTGTCATTTCAGCG
    ATGCTGAAGGGGAAGAGTAGCTATTTGGGGTCCTGTTTTCATTTGGGGCTCAAGCTGGCACTTTCTGTGG
    ATCATTTCATGAACTCCTAACCTGACAGAGTTATTTTTAATCTAGGAGTACAGGCTTGACAAAGTCAACA
    CAACTTGTCAAGGTCACATAACTAACAAGTGGCAGACTGAGGTCTGTTTGGCTGTAGAACCAAGGCCCTT
    TGCCCGTGCCCTCCTTGCCATCCCTGACAGCCACGCCATCTGTACTGTGCATTGTTGTTTCTGGGCATTT
    TTATTTTTACATATCATTTCTCTTTTAAATTAAAGAATAACAGAATGAAACTTGCAAAAGGGATGTCCAC
    TCAGGCACCCGCTGGCAGCAATGTTTGGAAGCATTTGCATGCTCTAGGGAGCTAGAGTATCCAGCCCAGG
    GTTCCTGTGCTCTTTGCACAAGACTTTCAGGACTTAAGAAAACTAATGAAAACAAACAAACAAACAAACA
    AACAAAAAAGAATTGTTAAAACATAGCATATGGAAGAATTGTGGAGTGAGAGGGAAACAAAGCCCAGCAT
    CTGCCAATCAGCCTGAAAGCCAAAGCCTGGCTACATACTGCACACTTTTTTGCTTGTGCAGATTTGATTA
    AGAGGCGACCTGCCATCATCTCCACCCTCTGCTGGTCTTCTATGATCTAAGATGTAGGTGATGCCGAAAT
    GAAGATTGTTCCTTGGACCATTGCCAGGGCTCCCTTCAGCCTGAAATATGGTTCACGTGTGATGCTCAAG
    TGCATCTGAGGCTGACAGAGGAAATGCTTGGCATGTAGCCAAAGGGGAAATCCCTGTGTTGAGAGCATAT
    GAAGAGATCACCAGGAGATGCCAGGAATCTCTGCCTTTTTTCCCTGCCCAGAGTCCTACCATCTGGGTTT
    CACAGCCGTGTATAATTGCATCACCTTCTCTGGGCATTTCTCTCTACAGCCTGGCAGATTTTTATTTTTC
    AACCCAGTGTGCACAAAACAGAAAGAATTTCAATTTTTTTCCTTAGCTTTTTACTAGTGCAAGGTATTAT
    ACAATGTCATCTCACTGCATGCCCAATAGCTCTTGAAGATCACATAAGCCTTATGTCTTTTTATATGAAG
    GCAGTCGGGCTCAGAGGAACTAAACCAACACCAAACAGCTACTGAGTAGTAGGGCCAGAATCCAAGTCCA
    TGTCTGCTTGACTCCAAGCCTATGAATGTAGCAACCAGCACCATTTCAGCTTGTACTTGTCTTGACTATA
    TGATAATTTGGACCTAGACATGTCCTGACTCCTAGTCCTGTCTCTGTCATGAAAAAGAAATCATAGCAGG
    GCCCTCAAGAGGTTAGCAGTACTTCTGTTCTCTCAAAGGTCGTGATTTCTGACCAAGGCCTGTTTATTTT
    TAAGGCATTTGCCTATAACATTTAAGCCTTAATTCATATGGGTTAGACTGTGGTCTCTCAAATCTTGTTC
    CTGACGGGCGGTGGCACCCTCCAGGATAGATGAAAAGTATTTCTGAAGGGAATTAGTAATCTACACTGAT
    TATCTCAACCACATTTATGAGTGAGGTATAATCTTTACAGTCTAAAGGTTTGTGTTGCCCTGGTGTTAAT
    CTAGACTGGGTTACAATACAGGATGGTTACTGACTTATCTATTTCATGATAGGTTAATCAATATGGTACA
    GTCTTCTGCCAAAGAACACCTTGAATTTTAGGAGTTCTGGACTCTCAAACCTAGCTAGCACATTTGCCCT
    CACAATGTGTTCAATGGATCATAACATCAGCTTTCAGCACCTCCAAGACATGTTAAGTCAGACACTCTGT
    TCTGGGCTGAGACCCAACAGTCTCCGTGAACCCAGGTTATACACACACCCAAGCTTAAGAAATGCTGCAT
    TTTGTTGTGAGGGAGAGAATTATTTTAGAATTTGGAGTAATCTCTGCCTAGGTTTAAAAAAACCTAATCC
    ACTACAGGTCCTTGTCAGAACTTGAAAGTTTACCCTGTGTCACCTTTCCCACACCTAAGCTGGTCCTCTA
    GCCTGGGGCATCCCAGTGCCTACTGCACAGCAGGGTGATTCGGATGCCCAGTGACACTGTTCTCGTCCAT
    ACTGGCAGCATGGCAGTACTTTCCCATTCTGTTCAAAGCTCTTTGGGTATTATGTTAATATTACAATCAT
    AACTAAGGAAATATCTGATGACTGTGGACCTGGGAAGCCACGGTCATCTCTAAGTTTATGTTTCTGAACC
    TTAAAAAAAAATAAATAAGATGGGTGTGGTGGCTCTCGCCTTTAATCCCAGCACTCAGGAAGCAGAGGCA
    GGTGGATCCCTGTGAGTTTGGGGTCAGCCTTATCTACATAGAGTTCCAGGACAACGAAGGCTGAAAGTCT
    GTCTTAAGAAGAGGGAGGGAGGAGATGATTGTGGATATCAGGAAGACAGTTCAGCAGGTCACATTACTTC
    TGCACAAGCCTGGTGACCTGGGTTTGAACCCTGAACCCAGAGTGAAAGGAGACAGTCAACTCCCAAAAGT
    TGTCCTCTGACCTCCACACATGCACACATACAATAGTCATACATCAATATATTTTTAAAAAACAAAATGG
    TATCAGGTAGCTGGGCCCTATGATTATTTAAGCTCCAGGTAGCCTCTAGTTCTTTATTTGGAGATGTTTT
    TACCCAAATCTAGAAGAGACCTCCTATAATCCTGGTCCAACAGTGTGAAAAGAAAGGGGAAAGTGAGTAT
    TTGGAAGGCAGATAAGCTGTTGATCGGGTTAATTGGATAACAGTTGACATTCTCTCTCCCATTTCCCCGC
    CTTTTCCTTCTCCTCTGCTCCCCTTCTTCCTCCCTCCTGCCCCTCCTCCACACAGATGTGTCCTCAGAAG
    CTAACCATCTCCTGGTTTGCCATCGTTTTGCTGGTGTCTCCACTCATGGCCATGTGGGAGCTGGAGAAAG
    ACGGTAAGCAGCCTTTTCCCTGTTTTCTTTGGAGCGCCAGCACCTAAACTTGATCTCCAGGCAGCCTGCT
    GGGAAGGGATAGTGAAGTCTGAAGTAGGCAACCGTGCATGGGATTGTCTCAGGAAAAATCCTCAGGGAAG
    ATGGAGGGTTAGCGTCTCTTCCTCCACCTATCTCTCTCCCTTGTTTGGTTTTCATGTTGGATGACAGGAA
    AGGAGTTTCAGGTGAACTATGGTGGTGTGTGGGATATGATCTTGGCCTTAGATACCAGAAAATGACTCTT
    CTAAACAATTGAAATTCCCTAATAGAGAATTTGTAACGTAGCAAGATTGTCTTCTACCCTCTCTTGTCTT
    GCATATTACAACGTGCTCTCAGTGGGCACCATGATGGTTGTCCTCTGGCTATTTTGGGATTGGCATTCAC
    ACTGACTTGGTTTCAGGTCATGGCTCTGCCAGGTATTGATACTGAACCTTTGAGGCTGGGTAGTCTTGTG
    ACCTCAGTTTTCTTTCCTTTTTTTTTCCCCCAGACAGGGTTTCTCTATATAGCCTTGACTTGTCCTGAAA
    CTCACTCTGTAGACCAGGCTGGCCTTAAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGAGATT
    AAAGGAATGCACCACCACTGCCTGGCTGACCTCAGTTTTCTTAATTGGGTGTTCAGTATAGCACAGCCCA
    CATGGACTATTGAATATGTTAATAAATACAGAAAATAACATATGGTAGAGTGAGCAAGCACTATTATTTA
    AAACAAAAAGTCTCTGAACTTTTCATTATCCCAATGGCAATAAGATTATTAGTAGACAAATAATGAAGGC
    ATAGAAGGGGGTGCTCTCTGTCCTCTGTCCTTGTGCCATGTCACAGTTAGCAAATGTCCATCAACTCAGT
    CCTCAGATGGTCGTCACAGGATCTCAAATGTTTCATGGAATACAAGGTAATCAAGTCAGGAATTTCTGCT
    TTTATAGCTACACTTTAGTAATAATTAATATTTATTTAACACTCAATTTCGCAACAACCATGTAAAGTAG
    GAGCTATCTGTATTCCATTTTACAGATAGTAAAAACTGAGGTTCAAGAGGCTAAGTAAGTTTCCCACAAG
    GTCCAGAATAGAACTTGATCCCAGTTTAGTCTCACTTCAGAGCCTGTGTGCTTAAATGCTAGATTTTACA
    AGTAAGGCGTGAAGCAACTATTTCCTGTGGCCATAAAGAGGAAATGGGTGTAAATTATAAATGAAAAGAT
    TTCCCAGCTGCTGATTAGAAATAATCTTCTGAGAATCCAGGAAAAAAAAAAAAGGTGAGCCTTTTTTTCA
    TTCTGAAGAATTACAAGAGTTTGAACGATATTCGCAGGTAAAGGTGCTTGCCACCAAGTCCGATGACCTA
    GACTTCATCACTGCGATCAGCATGATGGAAGGAGAGGATCGAGTTCTCTAAATTGCCCTCTGCTCTCAAG
    ACATGAGCTGTGACACCTGTGCACACACACACACACACACACACACATGAAAGAATTAAATGCAACTTTA
    AAAAGAGTAATCTCCAGCCTGGCCACCCACGCCAACAGAATTTTGGATGCTTTCCTAAGTAAAGCCTTTG
    CCACAGCCAGATGGACAACTTAGGCCCTGGCAGAGTTAACCCCTTAGTTACTTCTGACCTGATAAACCAC
    AGAAATTTCAGAAAAGCAACAGGAGAAACACAGAGAGAGCTTGTGTTGGAGTCACAATGGTGGCCCGAAG
    CCCAGCAGATATCTAAGCTCTGATTCCAACGTGTCCAAGAAGTTCTTTCCCTGCCCTGGGGCTAAGGTCT
    TCTAGGGTTTCTGAGTTCAGGTTCTAAAAGGAAAATAATAGACAGCCTGTTTCATGGCCTAGGGGACAAA
    GGGAAACTGGGAGCCAAAGGGTCCTGTGCACTGGAATCAGCTCAGGCTAACGATTTCTGAAGAGCTTGGC
    CTCAAGTGATTTGTACCTCTTATCTTTGCTCCCCATTCAGCAGTGACAAATCCCACTGATGTAAAAGTAA
    GAGCTTAGCATTTCACAAGGCTTTAAGCTCCAATGCCAATCACAAAGCCACCATTCACAGGGCACTTAAG
    GAAACTGTCTTAAGCTTGCATACAAATTAGTTTAGATGTTACCTTCTAAAGAAAGCACTAATATTTTGCT
    TTCTTTTTTCTTTTTTTAAAAGAATGACATCAGACTAGGGCATGGCAGTTCATGCCTGCAATCCCTGAAC
    TTGATAGATCAAGACAAGAAGAAAGCCCAATTTTGTGGCCAGCCGGGGCTATAAAGTAAAATTCTAAGTA
    GCTGGCTGTCTCTTCCCACCCCCGAGGCCATGGCTCTCTGAAAGCCCTTTGCTGGCTCTGCTCCATAGCC
    GTGCTATTCCAAAACCCAGGCTCTTCACCACTATGCCTTCCATATTCAATCAGGCATCAGATGACTGGGG
    CCATCCTGATCTGACTCAGCAACCAAGAACAAACCTCACTGATGGGTTTTCAGGTGATGCTGATGATGGA
    GGTTGAAAGACGACCCTGCTTTACGGGTCACTGCATTAAGCTAGTCTTTCGATTAATAATTGATATGTAG
    GTAACCTTTGAAGCTTTGTCACATGAGGCTTTGGTGTGACCCACTCCAGAATACCTTATGGAGATATTCT
    GGAGGCTCCTTCAGAAACTAGCATCAGATATCCATTGCACAACACAGGAGACAAAAGATCCGAAAGACAC
    CATTTGTTACCCTTCACACTTGATGGGACCTCACTTAGCCCTTGGCCATCAGCAAAAACAGTTCCTTCAA
    AAACTGAGACAATCATCTACTGCTTTGTAAGAGTGTAAAATAGCTTTTAAAAACAAGTAGTTAATGGCAA
    TCTCCGTCCACAAATTTCATGCAGAAGAGACTTAAAAAAAAAAAAACCTAAATCTTTCTGGATAATTTTA
    AGAGTTTTTCAGTGCATTTGCCTGGCTCTTTGGCCTGTGCATGACAGTGATGATGATGATGGTGGTGGTG
    GTGGTGATGATGATGATTTTAAAAAGCTAGCACAGGGTTATCCTGCCGTAACCTTAGCTGTGCTAAGGTG
    CATGCTTTTTTACCATCTATCCTCACAGCTAATCCTATTAGCATAGACAATATTGTTCTTACCATTTTGT
    GAGGACTCTGGGGCTACTGGTGGTTCAGGACCTTGCTCAAAATCTCCTTTGCAATTAGAGAAGATAGGAT
    TTCTGCATCTTTCTTTCTCCACACCTATGCTTTAAGCCACTATGTTATTCTGCTCAATACTCTGCCCTGT
    GTATTCCCTGTGAATTACAACTGGAAGCTTACTCCCCCTCATCTCCATTCCCAACACACACACACACACA
    CACACACACACACACACACACACACACACTAGTGGCTTCTGACAAGGAATCTTGTGCTGTTTGCATCTTT
    TCTTGTTTCAAGGTCTTCCCTTCCTAATCAGAGACCGATTATTACTGGCCACCTGCCAGTTAAATGACTC
    GGTGGCAGCTATAGAACTGTTTCTCTGTTCTGTCCCCATGCCCTGAGATCTCTCAGCAAGCACTTGCCAA
    ACTCCTGTGAGCTATGACGTTCCAAGGGCCATGGGTGTGTCAAGACACTGAGTGAAACCCAGTTCCCGGG
    GTTTGTGAGGCTCCTGGTCCAGGTCCTCACTGCTGGTGCTCTCCAGTTTATGTTGTAGAGGTGGACTGGA
    CTCCCGATGCCCCTGGAGAAACAGTGAACCTCACCTGTGACACGCCTGAAGAAGATGACATCACCTGGAC
    CTCAGACCAGAGACATGGAGTCATAGGCTCTGGAAAGACCCTGACCATCACTGTCAAAGAGTTTCTAGAT
    GCTGGCCAGTACACCTGCCACAAAGGAGGCGAGACTCTGAGCCACTCACATCTGCTGCTCCACAAGAAGG
    AAAATGGAATTTGGTCCACTGAAATTTTAAAAAGTAATTCCATCCCCCTTGACAGTATCTTTTCTTCTAC
    CCCTCCTTAAAAAGTAATAATTGCAAGTGTGTGTGTGTGTGTGTGTGTGTGCGCGTGCGCGTGCGCGTGC
    ACTGAGGCTCAGAAATGTTTCTCATTTCCTTTGAAACAGGAACTCTCATTGGCCCATAGCTTACCAATAA
    GCTTTCTTGGTTAGCCAATAACATCCAGAGACCTTGTCTCAAAAAAAAAAAAAAAAAAATCTCACCCTTG
    TACCATGTAATGGCCAGTGATCCATCCACAGTAAAAAAGAAATAAATAAATAAAATAAAAATAAAAAATA
    ACAAAAACAAATTTCTTTATTAAAAGAAAACTCCTAAATATTTAATTTTTCTTTTCTTTTCAAATGCAGA
    TTTCAAAAACAAGACTTTCCTGAAGTGTGAAGCACCAAATTACTCCGGACGGTTCACGTGCTCATGGCTG
    GTGCAAAGAAACATGGACTTGAAGTTCAACATCAAGAGCAGTAGCAGGTAAGGGAGCTGCCATGATTTCT
    CAAGGTTTTGTGTGATCTACTTCATAACAAAAAGAAGTACAAACTGCCCATGGATTATCAGTGACTGGTA
    ATGGAACAGCAAAGTCAATTCTGTATTTTTTTTTTCAACTTCCCTCTGCTCGCCTAAGGAAATAGCATGG
    AGCTTTAAGCCAGCCCTCTGAGACTAGTTGGCTGGGTAGTTTGAATTCACTCATCCGAATTGCCTGGCCG
    TGCTTCATTAAGGCTCACAGCACCATGGGGAGGAATTAAAAAGAAGCCACAGCTACAAATAACTGGCCAG
    AGCTCGACCTTGTGTCAAAAGCAGAATTGAGTGCTCCAGGGCCTTGCTGTTGGAAGCCCTGGAACAGATT
    CCAGAACCCTCTGAGGCCTTTCGAGATTTTCCTAGAGCCTTTTCAACTTCTTCCCCCCCCCACCCCCACC
    CCCCAACCGTTATTCTGAAGTTCAGTTTCCCCAATGCACTTGGGCTTTTCCTAAAAACATCATCTCCCAG
    GGCTGCAGAGCGGGTGTGTCAGACTGCTCTACAGCTGACTTTAGTCAGCAACTAAATCCCTGAGCCACAT
    GGAGGGCAGGGAGTGAGCGCCCTGGCTCTCCCACTATTTAAATGAATAAACGGGGGACAGGGGGTGTGGG
    GGGGACGACTCCCTTTTCCATCTTTATCATGAATTTGCTATGACTTAACTTAGCTAAGGAAAATAATGGT
    TCAGGGTGACATCAGAGTCCTTGTTGCTTCTATGAGAATTTTCTGATCTGGTAAAGGTGGCCCCAAAGGA
    GACCAGTGTACAATGCAGATGCTTACATATAGAAACATGCCAGACCTCTATGTGCTTTAAAAAATTAGAC
    AAGCACGATACATTTTCCTGCTAGAGAAATCTTGCACGTGTTCCTGAAGAAACTAGTACATGGATAAGGG
    TCAGTGGAACATTTTGCATAATTAGCAAAAGCATGCTCAAATGGAACATCAATGTGCATCTATTGAAATA
    AATAACTCAACTTTCGTGTTCTGTTTAGAGAACTCCCCCACAATACTTTCAGAGAGGAAAAAGACGTTAC
    AAACTCATGCATATATATTTTTCATTTGTCTTTAAAGTTGTGCTACTCTTTGGATAAACTACATATGTAC
    ATATATGTCTGTGTGCATGTCTATCTTAAATATTCATTTGGAAAAACTCTAGAAGTAACAAACTGTGAAC
    AATGTCTATATGTGGGACTCAGATTTGAGTAAAAAGGAGAATTGTGGCGACACGCATGTTTTCTTTATAA
    TCATCTTTGTTGTTATAATTCTTTATAATTCAGCAGTTTCTCTTGCATTACGATATTTTCAGGTTCATTT
    AGGCAGAGATGCAACTTTCAGTCTCTTACAGCTAAGCTTCCACTCTGCAAAGGGCAAGGGGGTTTTTGTT
    TGGTTTTGGTTTTGGTTGTTTTATTCCACTGGAGAAATCCAACCAGCTGGACAGACTGGCCTCGGACTGT
    AGACACCAGGCACTCTATCTCTGATCTATAATACAGAACTCATTCTGTCTTTGCCATCAGTTCCCCTGAC
    TCTCGGGCAGTGACATGTGGAATGGCGTCTCTGTCTGCAGAGAAGGTCACACTGGACCAAAGGGACTATG
    AGAAGTATTCAGTGTCCTGCCAGGAGGATGTCACCTGCCCAACTGCCGAGGAGACCCTGCCCATTGAACT
    GGCGTTGGAAGCACGGCAGCAGAATAAATATGAGAACTACAGCACCAGCTTCTTCATCAGGGACATCAGT
    GAGTTTTGCCTGGGAGCAGGTGCTAGGGAAGCAAAGACGATGTCTGCCACGTGTCCATGATACCCTGGGG
    CATAGGATTGGCCTGCATTCTTTTCCATTCTCCTAAAAAGCATGATACAGACACTACCCTCTTCATCTTA
    CAGATGAGGAAATTAAAGGGTTAAGCTAACCTTTTTCCAGATCTCAAATCTCACAGACAGCAGACCAGAC
    CCAGACCTGCCCCAATGGTGGCACCTAATGGATTATGGGTTAACTACATGCTTAGTGTAAAGCAGAAACC
    AAGCACAGGGAGGGTTCCCTGATTGCTGAGCCCAGATACGAGACTCTATTAAGCAGAATGACTCAACCAG
    TAGGGACTTCCGGTCTGTGCAGTGCAGAAAGGGTTCTAAAATGTAGGATATGGCCAATAATGGTGACTTC
    CTCGAGTGGAAAGATCACTCTCAGCAGATCCCTCACAGGTGCTGCCCTGATGCTCAGAGAAGTGAAAGAG
    CTATTCCAGAGACTAGAAGGTGGAGGGCTCTGTCAGCCAAGCTCAGGAGCCTAAGTCCTAGCTCCAAAGC
    CTGGCCAGGATAATAAAGGATGCTTGTCTTCTTCTGCAGTCAAACCAGACCCGCCCAAGAACTTGCAGAT
    GAAGCCTTTGAAGAACTCACAGGTGGAGGTCAGCTGGGAGTACCCTGACTCCTGGAGCACTCCCCATTCC
    TACTTCTCCCTCAAGTTCTTTGTTCGAATCCAGCGCAAGAAAGAAAAGATGAAGGAGACAGAGGAGGGGT
    GTAACCAGGTAGGATTTATGCAGCCCATGTTGGGGCCATTGGAAAGATCCTGACAACCAGGTGCTTGCCT
    CAGGACCAGAACTGTGTTAAGGGCTCTCCATGTGAATCACACACAACCTATACAAAGTCCCAAAGGCAGC
    CAGTATTGCTGTCACCTCTCCACAGAGGCCAGGAAAGCTCAGAGAGGCTATGTTTTGCCCACGTTCTTCC
    AGCTAGAGCAGTGGCAGAACCAGTACCCAGCAATGGTTTGATGGAGAAAGAGATGGTTAGCTCAACCACT
    TCAATTCCTCCAGTATAGTCGATTGTCAATCCTCAACCAAAGTTGTTCATGATATAGAAACCTGGGATCC
    AGGAGCCTATAATGATTGTTTAAACTGAGTAAATATAGTAAATGCTAAAACATTGATGCTGGGACCCACC
    AAATAAGGATAATCTCTACTTCCTTAATATGAGCAATCATTTCACCTTACAAGATGTCCACCTTTACTGG
    GCTAATATGGTGACTTTCTACCTTTCGGGTCCTTAGCACAGTGATCTCTTGCTAGGTTTGAGGAACACTT
    TTATGAGCATTTAAGTCACAGAGCAAAGGATTCTTCCAACCTAGCATTGAGAATGTGATAGCAATTCAGC
    TCAGAGATTCAACCACTTCCTAAAAGCAGGAGCAAAAAATGGGATTATTTTGAAACAGATTTTAACTGCT
    ACCTAAGTTTCATCCCTGTGTGTATGTATTTAATATACCAATCTTAAATAGGTAATGTCTAGTGCTTTCT
    ACTTGCTAAAAGCTTTCTACGCATTTTTACATAAATGTTGTATTTAGCTGCTGCAATTCTTAGAGGCAGG
    CATCCTGGGACCTACTCATGAATAAGGAGAGACACAGAGAACCAGAGATATTTTCCTGAAGCACCACAGC
    TGTTTAGTAGCATTGCCAGGATTTGAAGTCACTTTTGTAAGCTGACAGGCATGGTAATTCTTGATGTCAA
    GACAGCTAAAGAAAGCTGGTCTCAGTCCCAACATCAGCTTCATATGGGAGTGGTTACCCATGGGACCTTG
    GGGTAATGCTGCTACTTCAAGAGGCAGAAGGGGGAGCAAGCCATTTCTGTTGGAGTTTACTAAATAGAAA
    AGTGAGAGTGGGGTGGTGGATGAATCATGCTGGAATATGAAGACCTGAATTTTAACCCCCAGAACCTCTG
    TAAAACTGAGTAGTAATGCCTGAACCTGGGCTCTGGAAAGAGGTGGGACACATACACACACACACACACA
    CACACACACACACACACACACACACACACACACCTCTGATTATAGCTTCTACCAGCAGGTGGTGCAAGGG
    GAGCGCCCATGCCAGCCTGCCTTATATTGCCTAAAGCCCTTACTTAAATTTTCCTCTCCTTTGCAGAAAG
    GTGCGTTCCTCGTAGAGAAGACATCTACCGAAGTCCAATGCAAAGGCGGGAATGTCTGCGTGCAAGCTCA
    GGATCGCTATTACAATTCCTCATGCAGCAAGTGGGCATGTGTTCCCTGCAGGTGTGTGAGCAGGACCGTG
    AAGCCCAACCACACCTGCACATTTAGTGGTCCTGGTGGAGGACAACAGGAGGCTTGTGAGGGAGGTGGGG
    GTGGAATTCCTGGCATAGGGATTCCAGTCAGAAACACATTTGTTCATCAGCAGATGTTAACTTGATGTGT
    ATGTTAGGTCAGGCACTATGCTAGGCCAAGGATATTACAATAAAGAAAACAAGCATGGGTCCTACCCTTG
    TACAGGGAAAGGCAAGAAGGACCAGAGGAGGAGGAAATCAGGAGTCAGAGTATGGAGAACTTGGAAGTAG
    ACAGAAGTTGGAACCATATCTTAGGGGTTCTGGGGAACCACTGAAGTCTTTTTAATAAGGAAATATCCCA
    ATCAAAGCCCTTTTAAGAAAAATCTCTCAAGAGAGTCCCTGGCAAGCATGAGACCAGCTGAAGTCCAAGC
    CCTGTAAGGAAGGGTTGGGCTGTCTCGCACAGAAATCCTCAAGACCCAGTGTCTGGGGATAATAATGTGT
    GGTGTGAAATAGGAGGGTATTGAGGCTATACCTCTGACCAATATGGACTTACCTACCCACATTCCTTTAC
    AGGGTCCGATCCTAGGATGCAACGTTGGAAAGGAAAGAAAAGTGGAAGACATTAAGGAAGAAAAATTTAA
    ACTCAGGATGGAAGAGTCCCCCAAAAGCTGTCTTCTGCTTGGTTGGCTTTTTCCAGTTTTCCTAAGTTCA
    TCATGACACCTTTGCTGATTTCTACATGTAAATGTTAAATGCCCGCAGAGCCAGGGAGCTAATGTATGCA
    TAGATATTCTAGCATTCCACTTGGCCTTATGCTGTTGAAATATTTAAGTAATTTATGTATTTATTAATTT
    ATTTCTGCATTTCACATTTGTATACCAAGATGTATTGAATATTTCATGTGCTCGTGGCCTGATCCACTGG
    GACCAGGCCCTATTATGCAAATTGTGAGCTTGTTATCTTCTTCAACAGCTCTTCAATCAGGGCTGCGTAG
    GTACATTAGCTTTTGTGACAACCAATAAGAACATAATATTCTGACACAAGCAGTGTTACATATTTGTGAC
    CAGTAAAGACATAGGTGGTATTTGGAGACATGAAGAAGCTGTAAAGTTGACTCTGAAGAGTTTAGCACTA
    GTTTCAACACCAAGAAAGACTTTTTAGAAGTGATATTGATAAGAAACCAGGGCCTTCTTTAGAAGGGTAC
    CTAAATTTAAAAGAATTTTGAAAGGCTGGGTATCGGTGGTATATGCTTTTAATTCCAGCACTCAGGAGAC
    CAAGGCAGGCAGATCTCTGTGAGTTTGAGGACAGCCTGGTGTACAGAGGGAGTTCCAGCACAGCCAGTGC
    CACACAGAAATTCTGTCTCAAAAAC
  • Protein Sequence : Show Sequence
    >gi|6680397|ref|NP_032378.1| interleukin-12 subunit beta precursor [Mus musculus]
    MCPQKLTISWFAIVLLVSPLMAMWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIG
    SGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCS
    WLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELAL
    EARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEK
    MKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis FTL0552 mutant vaccine , Francisella tularensis guaB mutant vaccine
13. Il4 (interleukin 4)
  • Gene Name : Il4 (interleukin 4)
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16189
  • NCBI Protein GI : 10946584
  • Locus Tag : RP23-188H3.4
  • Genbank Accession : NM_021283.1
  • Protein Accession : NM_021283.1
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • Protein Name : IL-4, Interleukin 4
  • DNA Sequence : Show Sequence
    >gi|10946583|ref|NM_021283.1| Mus musculus interleukin 4 (Il4), mRNA
    GGATCCCCGGGCAGAGCTGGGGGGGGATTTGTTAGCATCTCTTGATAAACTTAATTGTCTCTCGTCACTG
    ACGGCACAGAGCTATTGATGGGTCTCAACCCCCAGCTAGTTGTCATCCTGCTCTTCTTTCTCGAATGTAC
    CAGGAGCCATATCCACGGATGCGACAAAAATCACTTGAGAGAGATCATCGGCATTTTGAACGAGGTCACA
    GGAGAAGGGACGCCATGCACGGAGATGGATGTGCCAAACGTCCTCACAGCAACGAAGAACACCACAGAGA
    GTGAGCTCGTCTGTAGGGCTTCCAAGGTGCTTCGCATATTTTATTTAAAACATGGGAAAACTCCATGCTT
    GAAGAAGAACTCTAGTGTTCTCATGGAGCTGCAGAGACTCTTTCGGGCTTTTCGATGCCTGGATTCATCG
    ATAAGCTGCACCATGAATGAGTCCAAGTCCACATCACTGAAAGACTTCCTGGAAAGCCTAAAGAGCATCA
    TGCAAATGGATTACTCGTAGTACTGAGCCACCATGCTTTAACTTATGAATTTTTAATGGTTTTATTTTAA
    TATTTATATATTTATAATTCATAAAATAAAATATTTGTATAATGT
  • Protein Sequence : Show Sequence
    >gi|10946584|ref|NP_067258.1| interleukin 4 [Mus musculus]
    MGLNPQLVVILLFFLECTRSHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCR
    ASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS
  • Molecule Role : Vaximmutor
  • Molecule Role Annotation : IL-4 plays an important role in Th2 immune response.
  • Related Vaccine(s): LVS
14. Nos2
  • Gene Name : Nos2
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 18126
  • NCBI Protein GI : 6754872
  • Locus Tag : RP23-341J22.2
  • Genbank Accession : AF427516
  • Protein Accession : NP_035057
  • Taxonomy ID : 10090
  • Gene Starting Position : 78920858
  • Gene Ending Position : 78960123
  • Gene Strand (Orientation) : +
  • Protein Name : nitric oxide synthase 2, inducible
  • Protein pI : 7.76
  • Protein Weight : 123528.57
  • Protein Length : 1144
  • Protein Note : Also known as iNOS; Nos-2; Nos2a; i-NOS; NOS-II
  • DNA Sequence : Show Sequence
    >gi|372099099:78920858-78960123 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
    TGGCCCCACGGGACACAGTGTCACTGGTTTGAAACTTCTCAGCCACCTTGGTGAAGGGACTGAGCTGTTA
    GAGACACTTCTGAGGCTCCTCACGCTTGGGTCTTGTTCACTCCACGGAGTAGCCTAGTCAACTGCAAGGT
    GAGTCTTGTGTTGGGGACAGCTGCTCTTAGGAGTGTCTTCCTGCTTGGGAAAGCCCAGAAAACCAGGCTT
    TCCCTTCTCCCCCCACCCCACCCCTCTTTTTGTGGCTGGACTGAAGCTGATATGGAAGGTGTAAGAAGCA
    CACCCACAGAAGGGTCTGCCAGTTGAACAGGGCGTGTGGTCAGATTTCTTCTCTGCAAATGTTTACTGTG
    GTGATTACACTACAAAGGATAAACTTTCTAGAAAGCTCCGAATAGGCTGGGGGGGGTGGTGTTAATACAT
    ATAGCTTACTTTACTCATCTTTATGAGATGTGCCCTCTGCCCCACCCAGATCTGATAAGATGGTTATTAT
    TAATCCCATTTTAGGAGTGAAGCAATGAGTTTCAGTTAACTAACTTGATTTAGATGAGTAACTAGAAAAA
    CTCAGGGTAGGGTCCAGTTCATCGGGACTCTGAGTTTCACTGTTCCCCTTAGGTGGGTTCTTGTACCCCC
    CCCCCCCGTATCTTCCAAGTATATCTTTCCTCAGGGAGGGCACTCGCCAAGGAGAAGCCACATACTCTCA
    GGGCAGTTCCACTTTCTATTTCTTGTCTTTTTCGTAAAGGATATAATCTCAACTCTGGAGAGTTTAATGC
    TCTTTTATACAAATCATTGACTTCTTCTCAAGTAACAAAGAAGAAGAAGGAACATGTGACCTAGTGTCCT
    TGGCTCAGGATGCGTGATGACTCCCAAATCAAAGGAAAGGTGGCCAGGACTTGAGTGCTAGCCCCAAGTG
    CAGGTCCAGAGGGAAGGGTACTAATGACAAGTGCTTGCCCCTCTCTCTGGTCAGCAGGTGGCAGGAAGGG
    TCAGGCAGATGCACACACTCCTGTATTAGTGACCCCAGCTCCTGCCCACCTTGCTGGAGGCCATGGAGTA
    ACTCCTGCCAAGAGAAACTGAATTGTCCTAAGGAAGCTTGTGGATGCTTGCTGTCCTTGGTAGGTGGCTA
    TGTGGCATCCCAAGTTATCCCAAGTTTGGTTTGTTATGTGTCTTTGTAGAACTGGAGTCTGTCCCACAAG
    ATTTCAACTCCAAATAAAACTGCCGAGTGCAAGCATGGAGAGAGAGCACTTCTGTGCTAGCCTTAGCTGG
    GCTCTGTCTCCATTCTCTGGGACACTAGCGCCCGCCCGTGAGAAGGCTCTGTTCTGTTAGGCTCTTCTTC
    AACCTCACCTCACAGCCTCCCTCCCCTTCCTCAGAGAACGGAGAACGTTGGATTTGGAGCAGAAGTGCAA
    AGTCTCAGACATGGCTTGCCCCTGGAAGTTTCTCTTCAAAGTCAAATCCTACCAAAGTGACCTGAAAGAG
    GAAAAGGACATTAACAACAACGTGAAGAAAACCCCTTGTGCTGTTCTCAGGTAAGCAACCTCTCTCCCAC
    TTCCAGCTTTCTCCTCAGGGCTTCCAGGCCCCTGCTGCCTCTCTCTGTTCTGGGCCACCTAAGAGAGTCT
    GGTCTCCAAGGCCCTGCTTGTAATACTCTACTGGAGTCTGATGCACCTTCCTTGGTGTGGACAAGGAAGG
    CCCAGGATCCCCATGCTGCTCCTGAGTGAGTCTGTACTCTCTGGAGGAAGAAGGAAGGGGGAAGGGGAAT
    ATGAGAGAACGGAGACTGGAGAGCCTAGTGGTCAGCTCAGTAGTGACGTCCTGATTGGCCAGGGCATGTG
    CCTCACCAGTTAGATATTTTGACTATTAGGCAACTGAAGGGTGTCAAGATGGCCCTGTACTATCCTCAGT
    GACTGTGTGACTTTGAGTAAGTGCACCTGTCTGAGTCTCAGGTCTGCAGTGTGATTTACCTGATAGTCAC
    AGAAGGGCTTGTCACCATTTAAGAGGCTTCTTTGTCACAGGACTACACCGTGCACACACCTACACTCTTT
    GAACGTAACCTTCCTGGGGCCCTTAGTTATGATCTCATTTTGCAAGTGAGGCTCTGGAGGGCAAAGTCTG
    GCTTACATGACTGCTAATGTCAGAGACGAAATCTGAGTTTGGCCTGAGGCATCTTAGAATTGAACTAGTC
    CTCCAACAATTTATCCGGATGGACTAGGAGAATTCTAGAGAATGCTAGGAAACTACAGCCATAGCCAGGG
    ACTTGAGGCTAACTTACGTAGAAAGATGATGAGCTGGCTTTCTTTGCAATGTGACTGCACAGAATCACGT
    CCAAGTCTCAGTCTGTACCTGGCTCAACATCTGCAGGACTACAGTGGACAGCGCCCCCTGGAGGCAGTAA
    GCAGGAAATCTCTAAATTTCCCTGTCCTGGTGTTGCCAGGTTTAGAAAGCAAGTTTAGGCAGAAGGGAGT
    AGCTTTGCCACTTCATAATCCTGTGGCATTTGTGACGTCCCTTAGCCTCCAAACCTCTGCCTCCTCACTT
    GCAGGAAAATAGTCATTATGTTAGGAGTAGAGGAGACAGATGGCTCAGGAGTGACAGGCAGAGTGAGTGG
    GAAGGGACTGGACCACAGCTGTCAGCAATCCTGGGGCTCAGGTGCCCTCTAGCACTTCAGTCTCAAAATA
    CCGTGACTTCATCCCGTTTTTTTCTCTTTACCCGGTCCCTACAACCCCTACCTTCTGTCGCCTCAGAATC
    CAGGTGCCTCCACACCTCTTTCCCTCCAACTTGACTTCATAGAAGTCATGCCCCTGCTCCAAAATCTTGG
    GTAGCTGCTGGTTACCTGTCATCACACTGAGACAAGAAGGAGGGCACATCCTCTGACAATCCAGAAGGGG
    ACTGGGTTGTCCTCCCACTTTAGGGAAGAGGAGACAAAAGTCCACCGTGCTTGAGTCATCTGAAGATGGC
    AGTGGTGAAGCAGGCATTCAAACTGGACAGTCTGGCTTATGGCCACCATCCTGGTAGCTACACTGTAGGT
    CTCCAATCGAGATGTATCCCAAGGACAAGGACACTTGAGGCTTTCTCACCTGGCACTATACTGCCAGTTT
    ACATGTCAACAGAGCCCTTCTGCTTCATCAGTGGATTGATGCAACCATTTATTAACTGCATATATGGCCA
    CACATATTTATAGTGTCCTTAACACTATGTCAAAAACCAGGCAGGAACTGCTTTTATCGTGTCTAAAGAG
    GTCAGTTTCTAGAAAGTGGAAGAGCTGAGATTTGAGCCTAACTCCTGTCTCTGAGGACTTAGTAAACTCT
    GGGCTGAGAAAGAGACAGATCCAGTCGCTACAGTCAGAGAGGAAACGGGTGGGGTGGGGGAGGGGGTTGC
    GCATGGAGAGCAACAGTTTCCTCCCTAGTTGGAGGCAGTCTTGGCAAGGCAAGATGTTAGAGAGGTTTAT
    GCGTCTCTGAGCCTCCTCCTAAGGGCACCGCCTGGCTGTGGGGTCAGGAGGGCCTAATGAGCTGCAGGTG
    GCTTTCTTAGAGGAGGGGGCCCAGCCGAGATGGGACTGCCCAGGAAAGGTGGCAAGAGGATGTTCCTGGA
    AGAGGAAGCCGCGTACAAAGGGGATTTTAGTCTGAAAGCAGTGGGAGCCCGTTATTTATTTTTATTTTAA
    GACATAATTTTCAAATCTATGCAGACGAAGAGCCTATTCCTGGTGGCTTTCAACTTTGTGTAAACAAGAG
    ATTCAGAAGTGTGCAGGGTGTGTCCTTGCATGTGTTTTAGAGACAAAGGATAAATGTGGGACCGTCTAGG
    TGACCCACAGGGAGTTTAGAGCAAAGGGTTTCAGGATGCTTAAAAGTACAAAACAAAACAAACAAAAACA
    CCCAAAACACTCCTCGCACTGTAATCGGGCTTGGGGATTCATTTGCTGAAATATTAGCCAGAAGGGTCCC
    TGAGGGCTGTAGCCCTATTTCACCTGCAACAGGGAGAAAGCGCAAAACATTTCCTGGGAGCGCTCTAGTG
    AAGCAAAGGTAAGAGATGACCCTAGGGCTGTTGTATGGCCTTGAGCAGGATCCTCATTTCTCTGGTCCCC
    CAGCTTCTGCCGCCATAAAGCAGGGGTAGTAAAGAATTGCCTTCTTAGTTGGATTTCGTTAGGGTGTGAG
    GACATAACCCGGTGCAGCGGGGAGTCTGGGTCGCGATCCTTACTGTCCCTTCGAGAGGAATACACAAACG
    AGGCACTCGCGCGCTCCTGCTTGTCCTGGGCCACTAAGAGCTGAGTCCTGCCCGCAGCAGGAGGGCGGGC
    GCTACGGGGGCGGGCGCTGAGGGGTTTCGCGGCGATAAAGGGACAGCGCCAAAGAGGAGCGCTGGCCCAG
    CTATGCGCGAGGGGCTGAGCAGCGCTTGCGACGCCGCCCGGCCGCGGCTCTGTCGCTTCCTGCGCAGGAT
    CTTCGGCCCGAAGGTCGCCAGTCGTGTGGGCCCAGCCAGGTACAGAGCGCGGGACCTGGGGAGGGGCCCG
    GGTGGCCCCGCTGGGACGCGAGAGAGGCGGGTCAGAGCGCCCACGCCGGCGGCCCTGTAAGGGTTTCTGC
    ACCAAGGAACTTTTGCGCTGCACTGCTTTGGACAGGTTCTAGCTATAACGATTACTTGGCGTCAAACCTG
    GAAATCTTCCTGCCTCGGCTTCTCAAATGCTGAGATTACAGGCGTGTGCCACCGCTTAAAGATGTTTTTT
    TGTTGGGGGGGGGGGGGGAAGCAGTACTGGGAACTGAACTGCATTTAAGCAAGTGCTCTTCCACTGAGCC
    ACTCTCCCCCTCCCGGCTTGCTGCTCTCCCTCCTTAATGCGCTCTGAACAAACAAGGTTAAGAAGCCAAA
    GATGATCTTTTACGATAAGACGTTCTCCCTTTCACAAGAGAGGTGGGCTGGCTGGTAGCAGTTCGATCCC
    AGGACTCAAAACCCTAAGTAAGCCCCTGCTGTGGGGTCTGCACTCACCCCTCAGTACCTAGTTCTGGGAC
    ACCGTGACAGCAGATGGTTCTATGGACCTGAGCAAGCCACCACAGTTCCCTTTGTCCGAAAGTCCATAAA
    GGTCGTTCTCCCTGCAATGGGCCAGTCAGGTCTCAGCAAGGCTGTGGGTTTAGAACTGCAGGAATTTGGA
    GCTAAGGGTCCCGAGCCTTAAAGTGTTCACCTGATATCTGGTACCTAGATTCCCTTTAACAGGTGGCAGA
    AGAAATCTGCTAGCAGGACATAATAGCTGGGGCAAAGCTTTATTTATCGTTGTGGGGTTTTCCCCTTAAT
    CTCTATTATAGCAGTACTTGAAAAATTATAAAAGTTCCAATTATAATAAAAACGTCAAAATACTTAAAAG
    TTAAAAAGAAAGAGATTTAAGTCACCCGTGGCTTATCACATCGTGCTAATCACTGTGAATCTTTGCTGGC
    ATTTCCCCCAGTTGCTTTCAGGCCTGCTGGAGCCTGCATTCATTCACACACAAGCACACATTGCATGCGT
    GCACAAAGACTGTCATCACAGCGTCACAGATATCCAGGCTGAGGCATTAGGCACAACCTCAGGCTGTGGA
    CCCTGAGGCCTGCCTAACTGTGGCTCATGTCAGCAGTGAGACTGCAGACAGCATTTAAAACATTTTGCCG
    TCTGGGGCAGGTTTACATCCTCACCAGAAGGAGGATCCCCAGAAAGCAAATCTTGGCTTCCGGATTTTTT
    GGGTTTTTTGTTTTTGTTTTTGTTTTTGTTTTTTTTCCTCCTTCTGGTGAGGTATACCAGTCCAGATCAG
    AGGAATATGTTAAAGAAAATATTATTCAGATGGGTGTGGTGGCACATGCCTGTAACCCCAACATTCAGAA
    GGTAACATCAAGAGTTCAAGGACATTGTCAGGCAAATGGTGAGTTCAAAGGCAGTGGAAGGTCATGAAAC
    ACTGTCACAAGCAAGAAGGAGAGAATTGTTCAATGAGTTTCTTGGTTTGCTTTGGTGGTGGCGGTGGGAA
    GTTATTGTTTTGCTTTGTTGTTTGAGATAGCCCCTCACCTGAGATAGCCCAGGCTAAGCAAGTCTCCTGC
    CTCACCTTCTGAATGCTGGGGTTTCAGACACCAGTCAGTGTATGTATCCCTCTTTAGTGATACCGGCTAA
    CATACCGCAAAGAGGACTTTCCTGAGGACTGTCCCTCAGGTGAGTCCAGGACCCACTGCAGTCCGTAGCA
    GAGGGAGAGATAGGGCTCCACCCAAACCCAGCAGGGGCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGG
    GCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGGGCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGGGC
    AAGGGCAAGTTACAGCCAAAGGTAAAGAAGAGCCTCACTGAACTGACCTAACAATCAAACCAGGGATCGA
    GTTATTACCAAAACACAAAGCAGGGCCATAGCTAAGCTGGAGTTTGGGGGGTTTGTTTTGTTTTGTTTTA
    GATTTATTTATGTGTATGGATGTTTTGTCTGACTGTATGGGTGTATAGCCATGGCTGCCCTGGAACTCTG
    TAGACCAGGCTAGCCTCGAACATAGAGCTCAGCCTGCCTCTGCCTCTTAAGTGCTGAGATTAAAGGGACC
    CACCACCATAGCTGGCTATATATATATATATATATATATACACACACACACACACATATATACATATATA
    TATATATATGCACACACACATATATATACACACACACACATATATATACACATATATATGTATATATGTG
    TGTGTTTCTTTTTATATTATATATATATAATTTTTATAAAATTAAATTTATTTTAATTTAATTTTTATCT
    TATTTTCAAAACAGGTAGCATATAGCCCAGGCTAGCTTTGAATTCCCTACTTAGCTGAGGATGGATGACC
    TTGAACTCCTGATCCCCCTGCCTCAACTTTCCAGGTGTTAGAATCATAAGTGTGTGCCTTCTGTACCTGC
    CTTTGTTCCTTTCACTCTTTACAAGCACGGGGCTTGAACCTGGAATCTCTGCACTCAAGAAGCCCTGGCA
    AGCGGATGGAGGCCAGTGCGGTGCACAGAGTAAACAAACGAAATCAAGGGTTTGGGGTGCTTTGTAGGTT
    CTCTTGGAGCCCAAACTGGCTTTGAAGTTTCTTTGTAGTTGAGGTTGGCCTCAAACTCCTGACCTTTCTG
    TCACAACCTGCCAGATGCTAGGATTCCAATTTGTAAAATGCCCAGCTTGAACTAAGAGATGAGCTGGGCC
    TGGTGATGCATGCTTTTAATCCCAGCACTCAGGAGCCACAGGCAGGTGGATCTCTGTGAGTTCAGGGCCA
    GTCTGGTCTACCTAGAAAGTCCCACATCTTAAACATACAATACTCACCAAAATGAAAACCGAAAATACAT
    AAGATAAAGTTTGCTAAGCCAAGTAGGTGGTGAGTGATGGACCCTGCTGGCCTGGGAGGGCTTTTGCACT
    TGGCCCTCTGAGGCAGGCATCTCCCCTGAACTTGTGTGTGTGCCTCTGTCTGAGTAGTTTTGGCTGGCTC
    CCCTCATATCATTTTGGCTTTGAGTGGTCAAAGCAAAGATGAAAAGGGAAGTGGGGACAAGATTCTCTAA
    GACTGGCTCCCTCATCCTTCCTGATTGGAAGCCGGGAACAGGTCATTCCCACCTCTAGCCCTTGGTACCC
    CGTCTGCCAGATCGGGACTCACAGAGGCCACTGGAGATCCCCTCCGTGGCCAGACTCCATCTCACAATGA
    CTGTTTCCTTTCGCAGCCCAACAATACAAGATGACCCTAAGAGTCACCAAAATGGCTCCCCGCAGCTCCT
    CACTGGGACAGCACAGGTCAGTGGTTTGGTCATACAGTATGGGAAGCCCTTCATTCTTTTCCTTGGTAGA
    TTCAGGCTAAACAGCCCTTTGGAAGCTGGGCAGGAAGGCACAGGAAGCCAAGGGCTGGGGAATAAGTGGA
    GCACCCCAGACTTAGGGTACAGAGGGAGCCAAGGAGGTTGCAGACAGTAGGACAGTCTTGATGCTCATCA
    ATAGAGGTCCCCAGGGTGCGCTGGTACCTAAGTAACTTGCCTGGTGGTTGACAGAATGGGTCGGGGTAGT
    GCCATTAGGGCTCTGTTTCTGTCAAGAGATAGCATGACCAAGGTAATTCTTTTAAAGGACTACATTTAAG
    TGGGGCTGGCTTACAGGTTCAGAGGTTCAGTCCATTATCATCAAGGTGGGAACATGGCAGCATCCAGGCA
    GGCATGGTGCAGGAAGAGCTGAGAGTTCTACATCTTGTTCTGAAAGCAAACTGGAGAAAACTGTCTCTTC
    CAGGCAGCTAGGAGAAGGGTCTCAAAGCCCACACCCAAAGTGACACACTTCCTCCAACAAGGCCACACCT
    ATAAGCAGTATCATTCCCTATGGGCCAAGCATATTCAAACCACCACAGGTAGTTTAGGAGCATTCTCTAA
    ATCAGGGAAGGACGGTTTTCCAGAATGATTTCAGATTCAGCTTCTGAGTCCCTAGTCTCAGCCCCTGGCT
    TCTTCCATCTTCCTGAAGCCCCTTACATCTTAAGGACTGTAGAGGGGTGGCAGAGAGATGGAGCCGGGAA
    AGGTGTCCCTGGGGAAGGGAGACAGTGGAGTATCTGTTGACTTCCCAGCTCTGCCCTTGGCAGTCCCAGC
    CACAGCTTCGGCCCTCCCATCAGACTTGAATTCCCCTTATGAGCCAGCACTGGCCAGGCCTTGGTAAGGA
    CAGAAGTCTGGCCTTGGTCCAGCCACCTGCAGTGCTCAGCTGGCACTAGCCCAACTTCTGGGAAGGGTGG
    AGGACCGAGGACTGGCCCCTTGCCTTGTGTGCTCACGCAGAGCTCCGTCCCTCATCCAAGCAGAGTACAG
    AGGCTCAGGGGACACTTGTATGGTTGTTGAGGTTATAGACATTTAGAAAACTCTTGATGTTTACCCATTT
    TAGAATGTTCCAGAATCCCTGGACAAGCTGCATGTGACATCGACCCGTCCACAGTATGTGAGGATCAAAA
    ACTGGGGCAGTGGAGAGATTTTGCATGACACTCTTCACCACAAGGCCACATCGGTAAGAAGTCTGGGGGC
    TGGGGAACACCACCTTTGTCCCAACTAGGCCATCATAAGGGCCCTTTGTGGGTCTATCACATATTTCCTG
    GTCTTATTAAACAGCCATCTTCTCAGACAAAAGATGTTGGGAAGTCTGTCTTTATAAATTATAGCAAGAA
    GAAGGAGAGGTTAACTACTGGGTTAGACAGCAGATAACCTAAGATGTCAGACAAAAAGCTACAGAAACCA
    GTAGAGTATCTGAGAATTGTCTATTAGAATTGCCCAAAGCTGGACTGAACTGGCAGAGGAAGCTTCCTGA
    CTCTTTAAGGACAGCAAGGTAAGCCAGGCAGTGGTGGCAAATGCCTTTAACCAGCACTTGGGAGGCAGAG
    GCAGGCAGATCTCTGAGTTCGAGGCCAGCCTGGTTTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACA
    GAGAAACCCTGTCTCAGGAAAAAAAAAAAAAAAAAAGACAGTGAGGTAACAGTGGTTTAAGCAGCCAGTG
    AATGAAACTAATCACCCTTTCAGGTTCCTTTCTACCTTTAAGTACCCTACTCTGACTTTGGAATTCATGC
    TATGAGGCTGAGCAGTGCACCAGTGCCCACCTTTAATTCCTTTGATGGAGACAAGCAGATGTCCATGAAT
    CCTAGACCAGCCTGGGCTACATAGTGAGTTCAGGGACAAGCCAGAGCTACACTGCGAGACCTTGTCTCAG
    AACAACAATAATAACAACAAAACATAAAATAAGCCAGGTGTGGTATACACCTTTAATTTCAGGAGGCAGC
    AGAAGAGGCAAGTGGAACTCTGTGAGTTCGAGGCCAGTCTGGTCTACGAAGGAGTCCAGGACAGCCAGGG
    TTCTGAGAAACCTTGTCTCAAAACACCAAAACCAAAAAATAAAAAAATGAAAAGCCCATAAAACAAAACA
    AAGGGGGGAGAGAGAGAGGGAGGGAGGGAGGGAGAGAGAGAGAGAGAGAGGGAGAGAGAGAGAGAGAGAG
    AGAGAGAGAGAGAGAGAGAGAGAATAATTCATACTGTGGTTATAAAGTGGGAAAGAATCTTTGAAGGCAG
    GATGATTTGCTGAACCCCCAGAAACTGTGTCTATAATGCCAGCTTGGAAGATAGAGGCAGGAGGATTGGA
    AGCTCACAGTGGCCTGCTATGTAGTGAGTTTGAGACCAGCCTGAGTCACAGATGATCTTGTCTCAAAACC
    CAACAGCAAACCTCAGCAACAAAGCAAAACAGAGAGACGCCACAGTTGACATGTGGACATCCATCTTACT
    GGCATTTCTTTCCAAGCCTCCCCTGACTTTGGAATAACCATTCTCTCACTCAGCTTCTCTGGGCTGACAC
    TGGAGCCCTGTTCCCTGAGGAGCTAGCCGGGTCTCTTTAGAAAGGAAAGCAGACCCCCTAGGGAAAGATA
    TCCCCTAGGAGGAAGCAGTAGAGTTTTCTCACAGGCTGTCACTGTATCTTTAGCTTACTGGAGAACAGAG
    AGAAGCCAGCTCACACCCACCTAGTCTCAGGAAAGGAGAGGGGAGCTAGGCTTGGAGAAAGCTTGGTTGA
    CTCCACGGAGAAGGAGCCTGCATGGGGTCATTGTGAGCATACCGGCCAGAGAATGGCCAGGCTTATGACT
    GGTTTGGAGCATGGGGGGTCTCGGTGGGATGAAGTGGGGAATAGGGGAATAGGGGCCCTACTCAGCCCAT
    TTCCTTTTCCAGGATTTCACTTGCAAGTCCAAGTCTTGCTTGGGGTCCATCATGAACCCCAAGAGTTTGA
    CCAGAGGACCCAGAGACAAGCCTACCCCTCTGGAGGAGCTCCTGCCTCATGCCATTGAGTTCATCAACCA
    GTATTATGGCTCCTTTAAAGAGTAAGTCTGGCTTTCGTCAGGGTGACATCCCCTCTGCCCTACTCCCTGG
    GGCTGACCTGTTTCCTACTGTTCCACTCTGATCGGTGAACTCTGCAGTCTCCAGACTTGATGGGGCTAAA
    GTAGGCTCAGGCACTGCAAAAATGTCTGAAAGTTTGGAGGAGAGTGTAACCGGTTTTGGATTCCGGCGGG
    CCACCAGGTCACTGCTGAGTCCTGGAGAAATCCATTGGGTCTCAACCTCCTCATCTGTAAAGTGGGCATC
    TTATAGCATGACTCACTAGACAGCCATGAGATGTGTACAAATTTAGAACAGTGTTCTAGCCTAGGGACTA
    TAATACAGTGACTTGGAATGAATCCTTTTATCCTGAAATAGAAGGACATTTAGAGACTGTCTGGTCAGGC
    CTCTCCTCCTACAGATGTGAGCAAGATTCCCAGAGACAGCACTGGCATCAGCCTAGTCTAGCTAGAGTCC
    GGGAGACCTGACTTGCTGGGGTGCTACATCTCTGGAGTGCTCTCATTTTATGCCATAATGCTTCCTTGAG
    TCCAGTGTCCAGACCCCTTTATTCTTTGGGGTTTTGTTGTTGTTGTTTTGTTTTGTTTTGTTTTGTTTTT
    TGTTTTATTTATTTATTTGGTTGGTTGGTTGCAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGC
    TGGCCTCGAACTCAGAAATCCGCCTGCCTTTGTCTCCCAAGTGCTGGGATTAAAGGTGTGAGCCACCACT
    GCCCGGAGAGACCCTTTATTCTTAAGCCCACTAGTCAGTCACTCCAAGCACCAAGTATTGTCCAGCATGA
    AAGGCAATTGTTTTTCATTGCCAGTTGCCAATGTTGTTGTCAATGCAGTTGCAATCATGGCATGGCTGTG
    TGGCAACTCTTGGTAAGAGTTGGGATCCAAGGAGCCATCTTCTGAGGTTCAAGCCTTGGTTTTCTTGCCT
    CAAAATATCTATTATAAAATACCCACCTTGTAGGAGTTTATATGAAGATTAAATGAATTAGAGGCTTCAG
    AACAGTGCTGGCCTGTAATGAGTGCAACCGAGATCTTGGTTACCTCGTACGTACTCAAGCTTTTAAACAT
    CTGTTTACTTGGTGGAGAATGGGGTACCTGAGCCACAGCAAGTTTGTGAAGGTCAGACAAGCTGTGGGGG
    TTGGGCATCTCCATCCACAGTGCGGACTCCTGGGATTGACTGAGGTCATCAGACGTGGTATCAAATGTCT
    TTACCTGCTCAGCCCCTCATTGGCCCCTTTACCCTCTGGCCCCTCATTGTTTTGGAGACAGCATCTCATG
    AAATAATGAAGGCTGGCTTCTGCCCACAATTGTTCTTGAGAGTGCTGGGGTGACAGGTACAGGGGATGCT
    GTCTAATTATAGAACATTGTTGACTGACTGATTTACCTGTCCAGTTGTAAGAATGACTGTCAGAGACAGC
    GAATGTCTACTCTGGCAGCTGGGGACGGTGTGGCAGTGTCATTTTGTGGCACAGCCTCACAGAGCAGTTC
    CACCTGGGGCGTGAGACACAGAAGGGTATCTGGTACCCAGAGCAGAAGTTTCCATTCTTGGGGAATCCCT
    GTTCCACCTCTGTACAGCCCTGGAGTGCCAGAGCCGGGAGGGCGCTTGACAAGAGGTCTCTATCGTCTAG
    AGTAGTGCTTCCCAGCCTTACCTGGGAGCTGGCAGAACAGTGATGACTGGTCCCCCCTGAGCCAAGGTCA
    ACCATCTTGGGAAAGGCTGTCTGTAGATTTGTGAAGGGTGGAGGCCTGTGGGGGCACTGTCTTTCCTGTA
    AGCATGTATCCGGGGCTTTGTGGCATTGAACTTCAGTTTCCCAGATCTTCCCACTCTGTTGTTAACAAAT
    GGCTGTTTGGGGGGTCTGAGATTCTAGATAGGCTTCATCTGGCCATTTTTTTTAATTTGGCACCAAGCAC
    ATGAAAAACTCTGAGTTGCTGGGTTAACTCTGAGAGCCAGGTGTGGGCAACACAGCTCTCTATAGATAAA
    TAGGAAGATCTTAGGAGGACTCCAAGTTCAAGGATTGCGTGGGTGACAGGGTGAATACAAGCCAGCCTGA
    GCAACATAGAGGGAACATGTCTCAAAATAAACGGTTCAAAAAACAAGTGCTGGGGCCACAGCTGAGCATG
    TAGCTCAGTGGTAGGATAAGCCTGGTTTGGCGCCCTGCTTTTCTTTTGAGGCAGGTACTCACTATGTTGC
    CCAGGCTGGCCTGAGTTTACCTAATTTACACAAACTCATTTGGAAATTACAAGTGGGTGTGGAGAGACGA
    AGCCTGCCTATGGTTTGCTCCACGTGCCGAGTCCAGAAGGCCAGAGAAACAACTGTCTCCCTCCTGACAC
    TCCTGTGAAAGACAGAGGCTCCTGTTTCTTCTTGCTACCATAGTTACTGCGCTTTTCATTACAACAAGAG
    GATGGCTTTGCTCAGGACCTGTCCACTCCTTAGAATACAACGGGAGAGGCCAAAACTCTTCCAAAGAAAT
    CAGGCCCACTCCTGGGTAAATGACACCAAGTTTTCCTCTTTCTCCTTGAAGCGGTGTTGCCAGACCACAG
    ACACTGCTGTTTTCGTGTTTGGGTAGAGTGGGGTGTATTTCTAGAATCTTCCTCTGACGACTAAGGACAC
    TCACCTCACCCAGAAGTTCCCCCTCACAGGGATCACTAGAGACAAACATCGCTGAATGTTTTGTCTTCAA
    GTCTTTCTGTAGTCAAAAAGGAGTTGCCAAGACCCTCCGCCTGCCCCGAAGGCTGTTCATTCCAAGTCCT
    CCTGCTGACCTGAATCCCAAGTTCATTCTTTAAATAATTTTGTTTGTTGCCATTTCGAAAGCCTCTGGTG
    GTGCTGGTTGGAAATCTCAAGGTTGCTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
    TTCAAAGTCTTTGCGACCAGGCTCCTCTCCCTTGGAATTATTTTGAAGTATGCTTAAATCAGTGAGTGTA
    GCTCTGTTGGGTGGGCTCTAGGGTCCTTCAAGTTGAGTCCTGACCTCAGGTCTTGTCCGGGCCTGCCTTG
    AGTTTTGAGGAGCCCTCGAGGCTTGCTTCTCCCCTTGGGTGGAAGGTGGGAGGTCCTATCCACGACAGAC
    AGTTTGGAGCCTGGGGCTGGCAGTGAGACCACCTGGAACTATGTCCTCTCTGTTACTAGGCTTGTGTTCT
    TGAGCCTGGGACTGTATACTCTAGAGTCTTAAGACTGCTGAGCAAACACAGCAGTGCCTCCTAAGTGACT
    TGTGGTGGCATGTAGCTGCACTCTCAGGTATTTGAGAGGGGGAGGCCAGAGGGTTGGAGGTTCTAGGCTA
    GACAAGACCTTGTTATGACTATAATAATCAGCAGGGTCCAGCGTAGGAACATAAGTGTGGTTTTTCTCCC
    AACAGGACTCAAGATGGAGATGGCAGAGTATTAAAAATCGGCCCCTAGTAAGCGAAGAGTGGGTCACAAA
    GCCAGCCCTACCGAGGACAATGTCCCAAGGGGCACAATTTGCCCTTCTAGTGTCCAAAATCAGTTCTTGC
    TTTCTGGGGATTGAATTAATCTCCATGTAAGGTCTTGCTATTGTTGGTCTTCCTGACAATTTCCCGTCCC
    ACAGGGCAAAAATAGAGGAACATCTGGCCAGGCTGGAAGCTGTAACAAAGGAAATAGAAACAACAGGAAC
    CTACCAGCTCACTCTGGATGAGCTCATCTTTGCCACCAAGATGGCCTGGAGGAATGCCCCTCGCTGCATC
    GGCAGGATCCAGTGGTCCAACCTGCAGGTGGGTCTTACCGGCAGAGCAGAAGGAGGAGCAGGGGTGGGCA
    GGCCCTGAGACAGAGCAAGTGGCCAGGGCTCTGCAGACTACAGCTAAACCCTGAGGTGAGGCATCCCAGA
    CCCCCTTCTAGGAGCCTAGAATCTTATTTTAGCACCAGGGTCTCTAGATGACCCACCCTATATAAAATGT
    TGGGGACCACATGCCATCATGAGACAACCAGCAACCTTGGATGTCTTATTCCTGAGGCCTTGGTGCTACA
    AATGTTTGCCTCCAGTGTTTGCGATCCTGGCCCTAGAGAGCATTTTATTTATCGTGGCCTCCTAGGCCAT
    CGTCTCCTTGGAGGGTCATTTGGGCAGGGGTGGGGGGTTGGGTCCTATTGAGTGCACTTGGACTGAGAAA
    ATCCAGGAGTCTCAATGGTGGTGCACTCCCATGGCCAAGATCCAGGCTAGGGCTGTGGGGATCCTGCCTT
    TTGGTTCTTTTCTTCTTTTAAGATTTATTTATTTATTTTATGTATATGAGTACACCATTGCTCTCTTCAG
    ACACACCAGAAGAGGGCATCAGATCTCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGA
    ACTCAGGACCTCTGGAAGAGCAGTCAGTACTATTAACCGCTGAATCATCTCTCCAGCCCCTTTTGGTTCT
    TTAAAGCCAGACACTTTTGGTAATTTGGTTTTACTGGGGTCTTCTGAATGGGATTCTCTGATCCCTTGTC
    TCAAGAGTAGGGCAGCCTAGAGAAGGGCACAGGGGATGCAGAGAGGAGGGTGTGACCCTGTGAGAGAAAT
    GCCCTCTGTCCCCAAGGACTTTGAATCAGCCCCAAGCCTCTGTCCTCACAGTCTTCCATGGTGTGCCCTG
    TGCAGGTCTTTGACGCTCGGAACTGTAGCACAGCACAGGAAATGTTTCAGCACATCTGCAGACACATACT
    TTATGCCACCAACAATGGCAACATCAGGTGAGGCACCTTTCCCAGCTGTGCTGGGCCCAACTGACTACTC
    ACTCTCAGGAAGAAGATGCTGGAGAGCACAGGAGAGGGGCCTCTGGCTCCAGGAGAAACTAAGGAGAGAG
    AAAAGAATCCAAGACTTAGCTTTTCCAGGACATGAAATGGCAGGGCAGCCTCCTCCCCATTAGTCTCTGT
    TATAGCCCAGCTCTTCCCCGACCCCTTTCTAAGTTAGAACCTCAGGGCTGTTACATCTTTAGGAAATTAC
    TTTGAGCAGGCCATGGTGACACATGCCTGTTATCCAAGCACTTGGAAAGGTGGAAGCAGAAGGATCAAGG
    GTTCAAAGCCATCCTTGGCTACATAGCACGTTCAAGGCTAGCCTGGGCTCCATGTGTTCCTGCCTTAAAA
    ACAAAATAAAACCAAAACAAAGAAAACCATTTTCTTAGTTGGGCATGGTGGTACATAACAGCAATTTCAG
    CAGTGGAAGGCTGAGGCAGGAGGATTACCATGAGTTCCAGGCCAGTCTGGGTCACATTGACAGACCTTGT
    CTTGTTTTTAAAAAGAATCTACTCAGAAGGCAGAGGCGGGTGGATCTCTGTGAGTTCAAGGCTAGCCTAG
    TCTATATAGTGCTCAGTCTGTCATTCACTGTCATGCAAAAAACACGCACCTAATGGGGATTCAGCACAGG
    ACTAGGCCAGGGGCCAGGGAAAAGGAGGTGACAGGGTGGGATACAGAGGCGACTGAGACCCTAGAATGCA
    GCTCCATCCAGCTGCAAACCTATGGTGCACAGCCACCAAGGGTGAGAATAAAAGGATCCAGCAGCGAAAG
    CCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAGGGACATGAGGCTTCC
    CAGAGTGGTGGCCCCTTGTTGGTCTTGATAGTTAGGGAGAAGCTGGCAGGAGGAGGAAGAGGAGGGTGAT
    ATCAGGGTGATTGGGATGCAGGGAAGAGTGGAGGGAAGTTGGCTGGGAACCCCTGACCCACTCACCCCAA
    CCTGGGACAGTTGATAAACTACTCTGTCCCTGTTCTGGGGAAGGTCGGCCATCACTGTGTTCCCCCAGCG
    GAGTGACGGCAAACATGACTTCAGGCTCTGGAATTCACAGCTCATCCGGTACGCTGGCTACCAGATGCCC
    GATGGCACCATCAGAGGGGATGCTGCCACCTTGGAGTTCACCCAGGTACTGACTCAGCCTCTCCTAGATC
    CCTGTGGGCAGGGGACCCTGAGCAAATGTGGGGCCTGGCCCTGCAGACTTACTGGGCCCCTCTCTCCCTC
    AGTTGTGCATCGACCTAGGCTGGAAGCCCCGCTATGGCCGCTTTGATGTGCTGCCTCTGGTCTTGCAAGC
    TGATGGTCAAGATCCAGAGGTCTTTGAAATCCCTCCTGATCTTGTGTTGGAGGTGACCATGGAGCATCCC
    AAGTAAGTCACCCAGGGTACGGGTTATATGCATGCAAGCCCATCTCAGGCAGCACCCACAGGACAGATGC
    TGCAAGGCCTCTTTGCCCTCTGCAGCAGCTCTGGCCTTTCCTCTCCAGCCTCGCTTTATGTGGAACTGCT
    GATGGTTACCAAGGGAACAGCAGCCTAGCAAACAGGGTCCTGGGAGAGTGGGATGCAGTGACTTAAGGAA
    AGCCCCTGAGCTTCTGTCTGAGCAGAACCAGGCTTTTTTCTCACCTCCCAACAACTTCTTTAATTTTTTT
    CTTTTATTTATTTAGTGTGTGTATTTGAATGTGTGTGCTTGTGTGTGTACCTGTATGTGACTGTATGTGT
    GCATAGGTGTGCATGTTTGTGTGTGTGTGTATATGATATGTGAGGTATATGTGGTATGTTTATAATCATG
    TGTATGTTGTTTGTATGTATGGTGTGTGGTGTATGTTTATGTTTGTAAGTGTGGTGTGCTTGTATGTTTC
    TGTGTGTGATGTGTATGTGTGGTTATGTGTGTGTGTGTGTGTATGGTGTGTGTGTGTGTGCTCAGAGGTC
    ACTTGCAGGAGTCAGTTGATTTGCTCCTACCATATGGGTCCTAGGGACTGAACTCAGCTTATCTATCAGA
    CATGGTAGCTAGTGCCCTTACCTGCTGAGCCACCTCAATGGCCTCCTCCCAGCTTTCTTAGGAAAAGGAC
    AACCCTCCCAGACAGAGGAAGTGTAGGCTCACAAGGACTTTAAGAGTGCAGCTGGGCCAGGCAGTGGTGG
    CGCACGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCGAGGCCAGCCTGGTC
    TACAGAGCGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTTGGAAAACAAACAAACAAACA
    AACAAACAAACACGAGTGCAGCTGGTTGAACCTCTGCAAAATGCCTCCATATGTGGAGGTTTACCAGCTA
    GCAGGTACTGACAGCTCCTGTCCTTTGAACACACTGAACTCAGTCCTGCCTCAGGACCTCTGTCCTGCTT
    CCCTGCCTGCTATGCCCTTTCCTCGGAATTCTGCAAGGCTCCAGTTTTCCCATCAAAAGGTATTCACTAT
    GACATCACTGCACCTCGGAGAAGTCTCTCCTGATCTCCTATCTTCAACCCCACACCACACTTCACTTCGT
    TTCTCATTTTCTCACTGGTGTATTTATTTACCCCTCATAGTCTATAGTCAAACGGTCTATAGTCTGGATT
    CTGGGCTGCAACTTTGGGAATAGAATCTTACTTTTTATCTATAAAGTGCAGGGCACGTAGTAGGTGCTTA
    ATTATAGATATTTAGAATGTCTAACTATACAATGAATGATACTATGTTTGCAATAATCCCATGAGTTTGT
    TGTTATCGACTCTGTGTCTATGATGGAGATACTGAGGCTCCGAGGGGCTAAAAGGACCCTGGTCAGTGCT
    TGAACCCCAGCTGTCTGGGTGCCAGAGTACTTTGCTGCTACATTATGTTGGAGTCTCAGCATTTAGTCCG
    ATATACACCTGACTTATTAGCATGAATGAGTGAGTAAAGAGATGTCTTCTACAATAAATGACCCTAAGTG
    TTCCCTGTTATCTCTCGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGACT
    GAGATCATCTCTTCAAAAGGACTCTCTAGAGCTCGGGCTATGGCTCAGCTGAGCATGATTGCTTAGCATG
    CTTGAAGCCCTAGCTTTGCTCCCCAGCAGTGCATAAACTGGGTGTCGGTGATACATGCCTATAATTCAGG
    CCCTTGGGAGATAGAATCAGGAGGATCAAAAGTTCAAGGCCATCCTCAGGTACAGAAAATCAAGACCAGT
    CTTGGAAATGAGACCCTGTCTCAAAACAACAAAGAAAAAGAAAAGGAAGGATTTCTTTGGTTGATCCTTT
    TACTGAAGTTGATTTTTTTTGATTGGGGAGAGAGGGCAATAAAGATGGCCCTAAGTGCAGCTGGCTCTGC
    CTCCCTGCCAGGTACGAGTGGTTCCAGGAGCTCGGGTTGAAGTGGTATGCACTGCCTGCCGTGGCCAACA
    TGCTACTGGAGGTGGGTGGCCTCGAATTCCCAGCCTGCCCCTTCAATGGTTGGTACATGGGCACCGAGAT
    TGGAGTTCGAGACTTCTGTGACACACAGCGCTACAACATCCTGGAGGTGATGAGGGTTACCAAGCTGGGA
    CAGCTGGAGGATCAGGATGGGGGACCCACTCCACCCAAACTGCCTCACCCTCATCACTTGGCTTCTATAG
    GAAGTGGGCCGAAGGATGGGCCTGGAGACCCACACACTGGCCTCCCTCTGGAAAGACCGGGCTGTCACGG
    AGATCAATGTGGCTGTGCTCCATAGTTTCCAGGTATGCCAGGTATCTTGGGGGTTGGTAGAGGTTTTGGC
    AAGAAGGTCACTTGATCCAGGTACTTGACTGGCACTTGATGTCCCCTTTACAACATCATTCATTCATGTG
    TGCATTCATTCATGCTTGCTGGGTGCTGAGACCCAGCATTATTGCTGGTCTAGCTCTGGGCAACTCCTGT
    CCTCCCAAGGCTGCCTGCTCCCTGCTCCTGCACTTGAGCACTGTAGGCAGCTTGGCTTCCTTTGCTCCAG
    GACATTGCTTAGAGACCCCTCCTCCCGGCTGCATCTCCAGGGCCTTCCTCCCTGCCCCATCATTCCCACT
    GTGCACCAACAGTTCCTGCCAACCTCTGGCTCCTTCTTCTTCTCCTGCTCCTGTTACAGCCAATGAGACC
    CATCTCCTCACACAGCCTCTTCCTCCGCCACTTGCAGCCTCCTTTCTGACTCCCTGGGACATCTGAACTT
    CTTGCAGGGATCAAAGACTCAGGAAAAAAAGGGATCCTAACACAGGACCATAGCTCTCCTAAGCACATGC
    CATGGTGTGTGTAAAGGTCAGAGGACAGCTAACAGGACCCCGTTCTCTGCTTTCAGTATATGGGTTCTAG
    GGATCAAACCCAACTTGACTGGCTTGGCAGCAAGCACTTTTACTCCTGAGCCATCTCGCTGGCCCCCTTA
    CTTTTCCTCCCACCCCGTTTCTTCTTCTTTTAAATCACCCCACGACCAGGTCCTTCCCTCCTTGAAGGCT
    TCAGTCGTTCATGTCCTTAAAGAACCTCTCCTGGACCAGGCCACTTTGGGCAGTGCTCCCCCGTCCCCCA
    CACTGTCTTCTTTCCTTCCAACTCCCTTAACTTGACTTTGTTGAAGTGGTTCCCAGCACCAGGTCGTAGG
    TTGACGACCCCAATCTTTGTTGTTTCTTTCCTCCGCTGGAAATGTGAGCTGCGCAGTGCTCAGTGCTCCC
    TGTGGCCGGGATTGTGACTGAGTTCACTGCTACACCTCTAGTCTAGCTCATGATGGAGGATTGGGAATTA
    TGGAATGGATGGACAAGTGGAGGTGAGCCGCCCCCGCCCCCCCCACACACACACATGAGCAGGGGCCTTG
    TGTGCTAGGTGGACATGGTAGCTGGTTCCTGTGTGAACTGAGCCTTCTCTCCTCAGCTTTGCTTCTGTGG
    TTCTGTTTCCTGTGGAGCCTGGATATGTGAAGAGGTGGGGAGTCCAGTCAGGGTCATTCATTCTTTCAAC
    GGGGATTTAACAAATGTTTGCCCCATGTGTGCCATGCATTAGCTATGGGTTCATACAATGTCCTCCAGGA
    CTGGGACACTCTCTGGAAGATTAATTTTGTGCTCACTGGAGTAAAGGGTGGTGAGCTAACACAAAGAAGG
    AAGAGCCCCACCCCCAGCCCCTGGGGCAGGCCCAGGCAGGAAGGACCACCCCTGGTTCATACATCCTAAC
    CCCCAGGGACTCCCACCGCCAACCCTGACTCAAGCAGGATGCAGATGTGTCCCAGCAAATGCACAGAGAC
    ATAGTCACCCCCTGATGATGTCCCTCTACTGCCCAAGTCTCCAGGCCATTCTTGTCACCAACTTCTAGGA
    CACCCTTCAAAAAGAGATGGCCTTTTTCCCTTATAAAGTGAGGGCAGTAGACAGGTGTGATGGAGCACTA
    CTGTAAGTCTAGCATTCAGGAGGCGGATGCAAGAGGATGGTGAGTTCAAGGCCAGCCTGGGCAACTCCAT
    GAGTTATTTCTCAAAGAGGGAAACAAAACAAAAAATGGGAATAGCGCTTCCCTCGATGAGTCATTGCTAA
    GATTAACTGAACGTGAGACACCTTTGGAGCTCTCTTGTTCCCGAGGGGTGCTCAGCATACGTGGTTGGTC
    CATCTATGCTTGTCGGAGTTATCATCCTCTCCTGACATCCAAGGGCACTGTATATGTGCACAGATAGTAC
    CTGGCTTACGAAGGCTCCACTTGGGAATTTTTTACTTTACAAGGAAGGATATGAAGGCACCGTGAATCTA
    GAGCAACTCTACTTGGAGTTTTTAAAATTCTTTTTTTTTTTCATTTTATTTTATGTGTATGGATGTTTTG
    CCTGCACCACATGTGTGTAGTACCTGGCCAGAAGAGGGCATTATATCCTCTAGGACTGGAGTTAGAGGAT
    TGTGAGCTGCCATGTGGTTGCTGGGAATCAAACCAAGATCTTCTGGAAGAACAGTCAGTGCTCTTAACCA
    CTGAGCCATCCTTCCAGCCCCCTACTTGGCTTTGTTGTTGTTTGAGACCTTGACTTCTCTCTAAAACTCT
    TTTTTTTTTTGGTTTTTTATTTTGAGACAAGGTTACCCAGGCTGATCTTCAGTCACTCTGTTGTCTAGGC
    AGGCCTTAAACTCAAGGCAGCCTCCAGAGTCTCTGTGCTGATGGGCCTGTATGGTTAACCGCATAAGGAA
    TTTTAATTTCGATCTTTCCTGGGCTAACGATGATGGCCGGATGCTCTTTTGAAGCTGGACAGCAGCAGCA
    AGCTACAGCCTCAGCCAGCTATGGCAGCTACAAGGACTAGAAGCTGACACTATAGTGTGTTGTACAGTCA
    AGCTACGATGGTTGGGAGGCAAAGTGCTTTATCAAAAAGACTTGAGATGGAGTCTCATGTACCCTAGGCT
    GGCCTCACACCGGCTATGTAACCTCTGATCTTTTTGTCTACCTCCGTAGTGCTGGGATGACAGGTGTGAG
    CTACCACATCTGAGTCATTTGTTTGTCTTTTTTTTAATGTAGTACTGGGCATTGAACCTCCAGGTCTTGG
    TGCATGCTAGACCAGCAGGCTATGTTAGCTGAGCTACACCCCAGTCCCCATTCGATGTGTTCTTGACTGA
    GGATACTTTCATCTTGTGATGGGCCCATCAGCATGGAGCCCTATCATGAGTCAAGCAGCAGCTGTATATG
    AGTTTTAAAGTGTGTGGTTTGACTCATGAGCCTGACATCAGGTGGCAGCTGCCAACCTTGCCTCATCCTT
    CCCCAGGAATATCCCTTCCTTGGCCAACTTGAAACTATTTCTTAAGACTCTTTTCAGCTGTCCTGACTCC
    CACCTCTCCTCTAGACCTATCCCAGACACTAGGGTTTCCCCCTCTGTCCCCTCTCTGTCCCCTCTCTGTC
    CCTTCCAAGCAGCCTTTGCTTGCCTCTGTCACAAATCCCACTAGGCCTCATGAAAATAGCTGATTTCCTT
    CAGCCTTTCCAAGAGCCTTGCTGTTTGCTGGCAATACATGGAGACTGTCTCTCCTCTGCCCCATGGTCTT
    TCTCAGTACCTGACAGATGAACAGGAGCAGATAAGACCCTTTGAGAGCACAGGATGCTTCAGTGTTGGTT
    CTCTCCTTCCACTGTGTGAATTTCTGAGATGAAACTCAGGTTGGGCTTGGTGACCAACGCCCTTACCTAC
    TGAGCCCTCTCCCCAGTTGTAAAGAAATTTCTCATGGGTGAATGGATCTTCAGACTTTGATGGGGCCGCT
    ACTTTTACTGGATCATCCCAGGCTAGTTTAGGCCTCTAGTTTGTAAGATGTGAATGGCTTGTCTTCCCTA
    CACTCCGGTCCCCACCGTCCCCACAAACCACTCTGGTGGTTTTGTGTTTGCTTGTTTTGGAGACAGAATC
    TCTTGGAGCCCAGGCTGGCCTCAAACTCCCTACGTAGCTGAGGATGACCTAGAACTCCCAACCCTCATCC
    TGTACACCAGAGTGATGAGATTATAGTTGTCTAGTGCTTCTTGTATGCCAGGCAAGCACTCCACCAAGTG
    AGCCTCCTTTAAGTTTGTATTTTCTTTACCTGAAACAAGATGGTGCAGTGTGAGCTGCCCACACCTGAGG
    CTCTCTGTCTGGTGGCGATGCATGGAGACCTGTGCATCGAATTTCTCGATGCCGCTTTTAGGGTAGTGGT
    TCTCAACCTTCCTAATGCTGGGACCCTTTAATACACTTCCTCATGTTGTGGTGACCCCAACCATAGAGTT
    ATTTTCATTACTACTTTATAACTATAATTTTGCTACTGTTTGAATCATAATGTAAATATCTAAAATGCAT
    ATCTGATAAGTGACCCCCAAAGGAGGGGGAGGGAGAAGACACATAGGTTGAGAACGTGGGAACCTCTCAT
    GTGACCGCAGGCTGGCTGCCATATGTGTGACCGTGGTGATGGCCTAGGTCTCTAAGTCTGTGGACTTGGA
    CCTGTGTGTGTTTCTGCAGCAGTCAGTGAGAACATGAGGAGCAGGGGCAGAGTCATCGCGTAGATCACTG
    GCCCTCCAGTGCGGCACTTCCCGCTCTCCTCTGCTGCTGCATCTCAGTCCACTCCATCTTCTTCTCCTTC
    AGAAGCAGAATGTGACCATCATGGACCACCACACAGCCTCAGAGTCCTTCATGAAGCACATGCAGAATGA
    GTACCGGGCCCGTGGAGGCTGCCCGGCAGACTGGATTTGGCTGGTCCCTCCAGTGTCTGGGAGCATCACC
    CCTGTGTTCCACCAGGAGATGTTGAACTATGTCCTATCTCCATTCTACTACTACCAGGTCAGGGAGAAGC
    CTCCACCCACCTGCCCATCCCCGCCCCACCTCCCATCTTATAGCTCAGCTGTGGAGACTCTGTGGTGTGA
    GGACTCAGGGCTGTACATGCCAGCTGAGTGCCACCTTGTGCTCTCTAATCTAGTGTACCCAATAAAAAGA
    TACTAAAAGCAGGTGGGCCTCTACCTTCTGTGACCATGACAAAGGAAGCCAAGTGCCCACTGCTGTGTGG
    CTCATGTGGGGCCCAGGAAGCAGTACCCTGAGCTTTGGCTAACCCTTGGTGGAGTGGGCCTCTATGAGTG
    CAGTAGAGGGTCCTCTGCCTCTCCAGCATCCCCCGGCCCAAGCCCAGCCAGAACCCCACCCCCAACTCTC
    CTTCCTTTCTGCAGATCGAGCCCTGGAAGACCCACATCTGGCAGAATGAGAAGCTGAGGCCCAGGAGGAG
    AGAGATCCGATTTAGAGTCTTGGTGAAGTATGTGGAAGTGGGCATGTCCCTCTGTCTGTGCTGTGTCCCT
    TTTACTGAGGCATCCTTCCTGACTCCACCATGAAGGGCAGGGCATACGGAGAATGTCCCTTTTGGTGTTG
    GCTCCCAGAACTCTAGACACCAATTCTGAGACTGATTTGCTTCTGTAGTTCAGATTTGCCAAAGAGACTG
    AGTCAGGATGCACAGGGGCCACCAGTCAGAAAATGAGAAGTAGAGGTTCTGCCCCCCACCCCAGGGATAC
    CCCTGTTCCGTCTCTTCGGGACACTAACTCACCACTCAGCTGTCTGTGTATCTGTCTGTTCATCCCCACA
    GAGTGGTGTTCTTTGCTTCCATGCTAATGCGAAAGGTCATGGCTTCACGGGTCAGAGCCACAGTCCTCTT
    TGCTACTGAGACAGGGAAGTCTGAAGCACTAGCCAGGGACCTGGCCACCTTGTTCAGCTACGCCTTCAAC
    ACCAAGGTACCCACTTAGGTGGAAGGAGTCTGGAGTGAGTGTAAGCGGCCCCTTGGGAGCTGGGGGCTGA
    TGTTCAACGGTTGTCACACAGGTTGTCTGCATGGACCAGTATAAGGCAAGCACCTTGGAAGAGGAGCAAC
    TACTGCTGGTGGTGACAAGCACATTTGGGAATGGAGACTGTCCCAGCAATGGGCAGGTGGGTGGCCGGAG
    ACTCGGCAGAGAGGCTTGGGAGGACCAAGGAAAGGGTCCGGGGGACTCAGCATGACACTCTGGGTTCTTA
    TGCCACTTTCCCAATGGCACACAGTGTTTTCCTGTGAATTGACCAACTCTTACTTGTATGTGTTTAGATT
    ACTTGAGGGGATTCATTACATTTATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
    TGCAAGTCCCACAGCTTGCATGTAGAGGTTAGAGGACAACTTGAAGGAGACAATTCTTACCTTCTGTAAC
    ATGAGTCCCTGAGTTTTTCAGGCTTGGTGGCACTTTTACCTCCTGAGCCATCACTGCAGCCACAACCTAC
    TTAAAAAAATTTTTTTTCCAAAACTTCTTATTTAACTGGGCATTGTGATGTACACTCGCAATCCCAGCAC
    TAGGCAGTAGAGGCAGGAGGATCAGAAATTTGGGCGCAGCCTGGACTCCATAGGAAATCTTGACTCAAAT
    TCTCCCTCAACCAGGATTCCTCCTTAGGGGCTGAGAGATGGCTCAGCAGGTAAGATGGATTGTGGCACTT
    TCAAAGAACCACAGTCCAGTCCCAGCACCTATGTCAGGTGGCTCATAGTCTCCTATAACTTCAGCTCTAG
    GGACTCCAACATTGTCTTCTGGCCTTCCTAGGCACTCACACTCTGTACATAGACACAATCTCTGACACAA
    ATCTTTTTTGGGGGGGGGAGGGGTAAGGTTTGTTTGTTTGTTTATTTATTTAATGTAAGTGAGTACACTG
    TACAGATGGTTGTGAGCCTTCACGTGGTTGTTGGGAATTGAATTTTTAGGACCTATGCTCGCTCCAGTCA
    GCCCCCCTCACTCTGGCTCAAAGATTTATGTATTATTATATCTAAGTACACTGTAGCTGTCTTCAGACAC
    ACCAGAAGAGGGCGTCAGATCTCATTACGGGTGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTC
    AAGATCTGAGGAAGAACCCTTATTAAATTGCTCTTACCTTCTGAGCCACCTCACCAGCCCGACATAAATC
    CTTTAAAAAGAATTTTTTTTTAAATGTATTGTGGCTAGAGAGATGGCTTAGCTATTAAGAGCACTGACTG
    CTATTCCAGAGGTCCCGAGTTCAATTCCCAGCAACCACATGATGGCTCACAACTCACAATGTAATAAGAT
    CTGTTGCCCTCTCCTGGTGTGTCTGAAGACAGCTACAGTGTACTCACATACATAAAATAAATCTTAACAA
    ATGAGCAAAGACCGTATCATTCTCACAAAGGAAAAATGGGCCGAATATATGAATTTTGTCTAGCTACCCT
    CATGCAGGCCTGCACAGCCTTGTGTTTTGAGCAGAGACAAGTATGAGCAAGATGTTAAGGCTTGGTTAGG
    ACTAATTTGAGTTAGTCTCTCCTGTGTTGGAATGATGCAAAGGCCATCGAAAAGAAAGTGCATGAGAAGA
    GATTACAAAAGGCTTAGCTTCCCACAGTGTGATGAGTCCCACACTGCCTGAGACAGTTTCCTGGAAAATC
    AAAGTTATTTTAGAGAATCTGTTGTTTCTAGCAACTTCCAAGCAAGAAGCAAGGTTCGTCTTCTTTTTGA
    GGAACCTTGTGTTTTCCAAAGCTAATTGGGGAGGCTAGGGACTTCTGGGTCTTCCATTGAAGGGAAGGGC
    CCACGGGAGCTGGTTCAGCTGCAGTGAGGGGCAATCTGGACTTGTCCATTGGCTTTCCCTTAGTATCTGG
    ACTCTTTGCCTGCTCAGTGTGGCCAGTGTTTACACAGACCATCATAGTAAAGGAAAGCAGTGTTTTTGTA
    GCTCCTGGCCTGAGATCTCCGCTTCCTTGTCTTTTGTAGACTCTGAAGAAATCTCTGTTCATGCTTAGAG
    AACTCAACCACACCTTCAGGTGAGGCATCTCCCATATCCCTGACTCCTGGGCCCCAGTCTGGGATCAGAC
    CAAGGAACAGGGTGTACACAGGATGTCTTCTCAGACATGTCCAGGGGCAGACAGAGTTCCGGTCTGAGGT
    TTGTCTTCTGAGTGGGTTGAGGAGCAAGATGTGGAAACTGAGTCCCTCTTATAGATTTGCAGGCCTAGAG
    CCCACTGGTCACATGACCAAGGAAGGCCAGACTACCGTGTGACTCAGTGGGCATGGGTCTGTCCTTGCAA
    ACCATGGGCATTGATGCTGGGCATCCTGAGTCTCCATGTGTGTCCTTCTTGGCAGGTATGCTGTGTTTGG
    CCTTGGCTCCAGCATGTACCCTCAGTTCTGCGCCTTTGCTCATGACATCGACCAGAAGCTGTCCCACCTG
    GGAGCCTCTCAGCTTGCCCCAACAGGAGAAGGGGACGAACTCAGTGGGCAGGAGGATGCCTTCCGCAGCT
    GGGCTGTACAAACCTTCCGGGTGAGACAGGCTTTAATGAGTCTATGCCAAACACATCTGGGCCTGGTGTC
    CTGGGTGTCATGGCCTCTGCTAGGTGTAGGGCGGGGGTATGGGCCTGGCATCTCAGGTCAAAGCTGCTTC
    CCCAACCCTCACCCCACCAAGAAAAATGTCACTCTGGTGTTCTGCTTCAGGTGGGAGGTGATAGCTGTCC
    AGCTCCGGCCCTGACCTTATGTCTCCAGGGCTCTGAAAGCTTGGCCACCTCGTAGTAGTGAAGTCTTCCT
    TCAGTCTTCCAGCTCTGCAGAGGAAGGGACACAGGACACACTGCTGAGCTCTCGTGACCTGACTTTATCT
    TCTCAAATCTTTACTCTCTGGGTTCCTCCCTGCTGGTAAAGGAAGCTTAACACTGAAATGAATGGACAGC
    CATGGGAATCCCAGCAGACTTGATTCCAAGACTCAGGCCTCCTCTATGAGCTTTTCTATCAGGATAGGGA
    AACCCCAGACATCCCTTGCTATGTGGGAAGGGATAAATATAAGATGACAGCTTTACTATCCAGGAAAATA
    TCACTAACCACTGTGTGCCAAGCCTAGGATTCTCTGAGCGCCTCCAGCCCCTTTCAGATGGCACGTTGTG
    ATTCTGTGATGCCGCCATTCGGTATTTGACCCATGGTGAGGGGTGGTGACGGTGACGATGAATGCCATTC
    TGCCTAACTTTCCAGGCAGCCTGTGAGACCTTTGATGTCCGAAGCAAACATCACATTCAGATCCCGAAAC
    GCTTCACTTCCAATGCAACATGGGAGCCACAGCAATATAGGCTCATCCAGAGCCCGGAGCCTTTAGACCT
    CAACAGAGGTAAGAGCCCCTCTGCACCCTACTGGGCCTTCCCTGCTTGAGCTGAGGCTATCTGAGCCCTG
    AACCAGTAGTCAAAGAATCCACCATGCCCTTCTAATGAAGGCAGTCACCTGGAACTGCCAGGCAGCTGTC
    TCCCTGGCCACAGGGTCTGTTGGTACAGAGCTTCAGGCTCTGAGGGAGAGCGGGTCAGGAGCCCTGAGAG
    AGTGGTAGTGGGCACAGGGAGCTGGCATCAGCAGGTGGGTGGTGGCGATAGCTGTGCCAGGCACTGTGCC
    GGCCTCTTGGCCCACATGAACTGCCCACCAAGGGCTAAGGTAGCATGCAGGAATGCTCAGCTCAGCGTCT
    GGGGCTTTCCGCAGCACAGCACAGGAGATCTGTACCATTTCTGTCTGTCTTTCCAGCCCTCAGCAGCATC
    CATGCAAAGAACGTGTTTACCATGAGGCTGAAATCCCAGCAGAATCTGCAGAGTGAAAAGTCCAGGTAAG
    GATGGGCAGGGATGCTTAGCTGGTACCCAAGATTGCCCGCGAGATGCTTCTTGTGACAGAAGTGTGGCCA
    AGTACTCCATGCTCCCTGCACTGCTAGGGAAGGCTATAGCCAAACTCCTCTGGGAAACTCCAGAGTGCTG
    AGGGTCCCCAGCTGAGGGGATGGCTTGTCACAAGCTGGCTGGTGACCACACTTGGTGGTGGGAGAACACT
    TATCCTGGGGTAACTGGCAGTCACCCATGGCTCCCTTTTCTTCTGTAGCCGCACCACCCTCCTCGTTCAG
    CTCACCTTCGAGGGCAGCCGAGGGCCCAGCTACCTGCCTGGGGAACACCTGGGGATCTTCCCAGGCAACC
    AGACCGCCCTGGTGCAGGGAATCTTGGAGCGAGTTGTGGATTGTCCTACACCACACCAAACTGTGTGCCT
    GGAGGTTCTGGATGAGAGCGGTGAGCCCCCGGCCCTGGACAAATCTCTACTTCCCTCAGTCCCATCCCTG
    AGAACTGGGCCTGTGTCTGTCCTGATCTACTTCCATCCCAACTCATGGGAGAAGCTTTCTGGCCTTCACT
    GGTAGTGAGGCCTCCCCCTAGCCTTGGACGAGGCACTGGAGGAGCAAAACCTGGCAGTGTCCATCATCAT
    CCAGGAGAAAGGGAGTCAGCCTGAAACTTAGAGACAAGAGACTTGCATCCTTGGGGCTGTGAGAATACAG
    ATGTGGTCCAGATGAGATGCATTAATAATAAAGTCCTTGTCTCTAGAAGGAAGTGAGGGGAGTGGAGAGG
    AGTTAAAAGCAATGTGCTAACACGCACTAAACGTGCCCAGGGAACGGGCACTGTGCCAGCGATTTCCCAA
    ATGTAGTTTTTACTCATTGCCAGCACAGACTATGTCATCACATCTGTTTCTCAAGAAGCAGCTGGAGGTC
    ACAGGATTTGGACACTCGGCTGAGAAGTGGGAACCAGAGTTTGCGCCTGGGTCATGCCGTGACTCTCCGA
    ATGCTGCCTCTTGGGACTCCTGAAGCCCAGCCACCACAATTTACATTGATCTGTTAATATCTCAGGAGGT
    TGTAGCCATAAACCTCTCTCTAAAAAAGCAACCAGCCTCTGGGGCGTATATCAATCTCCAGTAGACTTAG
    TCACTAAGTTTGAGGTTCACCTGGGGATGTCAGAGACTCAAGTATGGGCCACAGAAGGATTGCTTAGTCT
    GTCAGGACAAAGCCAATATAGGTGCCCACGTGGGGGCAAATTTTCAGCCTACCCTGGCAGCCCTGTCTTG
    GCACCAGCTCCTGATCTTTGCTCTTTCTGGAGCAAAGATGTCAGAGTCTTCCCTCCCTTGCAGATGGAGA
    ATAGCCATCATCTGAGAGACCCAAGTAGGGCTTTCCCCACCCTGACACCTTTCTTTCTTCCATTCAGGCA
    GCTACTGGGTCAAAGACAAGAGGCTGCCCCCCTGCTCACTCAGCCAAGCCCTCACCTACTTCCTGGACAT
    TACGACCCCTCCCACCCAGCTGCAGCTCCACAAGCTGGCTCGCTTTGCCACGGACGAGACGGATAGGCAG
    AGATTGGAGGCCTTGTGTCAGGTGAGGCAGGGGCAATTCAGTGGAAGGAATAGAGCCCAGAGCAGAGTTC
    AACCTCTGAGACCATCCTCCTGAGTCCCTAGGTGTGCCTTGTCCGTGTCTGGTCGGGGCTAGGCGTCGTC
    TTCTGCCCCGTCTCTAGTACACAGCAGTATTGAGTGAATGAGTTTCTCTGGGAATCAGTATGCTGTAACC
    TGTATTCATCACATGTCCCATTTCTGTGACTAACTGCATGTCAGAAGGAACTTAAGGGAAGGAGGGTTCA
    TTTTGGTTCATGAGGGTCCAGTCCATCATGGTAGGGTGTCATGACAACAGGAGCATGAGTGACAGATCAC
    ATTGCTTCTGGAGTGAGAAAGCAGGAAAGGTGGACACCAGTGTCCTGCTCAAAGTCCCAGACCATAGGAA
    TTCGGGGTGGGTCTTCCCAGTCAAACCTCTAGAGAAACACCTTCACCAAGGTGTGTTTCCACAGTGATCT
    GAAATCCAGTCAGGTTCACTGTGAAGAGTAACAGCCAAAGTGCACTTCCTTAGAACCTGACTAAAGGGGG
    AAGCTCTATGGATGGCCTTTGTCTTCCCAAGTGTGGACCACGCTTGGTGGTGGGTAAATGGGGGTACTGG
    CGGGTGGGGAGCTGAGTTAATGAGTTAGTGGGGTGATGCATTAAGTCACTTGCGACTTTAGAGTCCATGT
    CTTTATAAGAGTCACTGGACAGCAAGCCATGGGACACATTGTCATTGTCTCAACAATCCTATATTAATGG
    CTGCATCCACCTAACTCCACAAGGATTAGGGTTAAGCTAAGGAGGGACATCCAGCCATGACTTGAACTCA
    GATCCAGTTCGTTCCAAGTTACAGTATAATAGAGAAATTAGCCCAGCTGGGTTGGGTGGTGCATTCTTGT
    GATCCCAGCACACAGGAGGCTGATGCAGGAGGATAGTGAGTTAGAGGCAAGTCTGGGTCAACAATGAGTT
    CAAGACCAACCTGGGCTATGTAGCAAGACCATGTCTCTACATCCTCCCCAAAGAAAGAGGACACTGAGCC
    CTGCCCATGAGTTGCTGATGTTGGGAGGCTTCTGTGTTTTGATTTTTGTCTTTGTTTTGTGGGTACTAGT
    GTTTTCCTTCAGACCTGCACACATGCCAGGCTCTACCACTGAGACAAACTCTCTCTTCTCTGACTTTTAA
    TTCTGAGGCAGGGTCCCACTAAGTTATCCAAGCTAGCTCTCAATTCGGTCTCTAACCCCTGCTGGTCTTA
    AACTTGGAATTCTCCTGCGTCAGCCTTCTAACTAGTTGGGGTTGCAATCCTGCCCCACTGTCATCTTAAT
    TCTATTCATAGTAGAGTTCTAGGCACAATTGAGAAATGCTACTAGCTGCCCCAGGTCCTTGCTTCCCGTC
    CACCGGAGTAGCCTCAGCACCCTGGATAGCGCCCGTCTTTTGCTGCGTTTGTTGAAGGAATGAATGGATA
    CTGGGTGGGGCAGCTCCTACACGCACGCAGCTCCTACACGCACGCGCGCACGCACGGCAGGTCCTCCGAT
    CCCTCACACTGCCCTTGCCTTTCAGCCCTCAGAGTACAATGACTGGAAGTTCAGCAACAACCCCACGTTC
    CTGGAGGTGCTTGAAGAGTTCCCTTCCTTGCATGTGCCCGCTGCCTTCCTGCTGTCGCAGCTCCCTATCT
    TGAAGCCCCGCTACTACTCCATCAGCTCCTCCCAGGACCACACCCCCTCGGAGGTTCACCTCACTGTGGC
    CGTGGTCACCTACCGCACCCGAGGTGAGACAGGCAGGCTCTGGTGTCATCTGCACCTGTCCTTCTAGGGA
    CAGCTGTCCTCGATTGCTTTCTGTTGCTGTGACCAACACTGACTGCCACCACTTTAGGGCAAGATCCAAT
    TTAGGGGTTTAACTTAAAGTTTACAACTGTCACCAAAGGAAGCCAAGGCAGGAACCACAGAGGAACGTTG
    CTTATTGGCTCCCTCTGGCTTGCTCAGCTGCCTTCCTTACCAGCTCAGCACCACCTGCCCAGGATCAGCA
    CTGCTCACAGTGGGCGATGCTCTTCTCCATCATCAATTAGCAGTTAAGAACGCACCCCACAGACATGCCG
    ATAGGCCTATCTGATGACAAGCCCTCAATTGAGGGTCCCTCTTCCCAGGTGTATCAAGTTGATGGCCGAC
    ACTGGTCACCACAACAGCTGTTCAATACCTTGGGGCTTTGTCAGCTACATGCTGCAGTGACAGCCACCTG
    CCAGAGTTGTCAGTGATATAAGATGCTGTGTAGGGCACAGTGTCACAAAGCAGGAGGGAAGCTCGCTCCT
    TTGTTTACCATTCAGACCTGTAGGAGATGGCTTCCATGTGTAATTTTACGAGGAAGGAAAAGAGTCATGG
    AGAGGTTAACCCACCTGTTCAGAGACACCTGTCATTAACTAGAGACACAAATTCAGGGAGTTTACCTGCT
    GAAGCTAAGAACTGGACCATTGTTACAGTCTCTGGCTGTTGAATAGGCTCAGACGTGAAAAGCCTTGAAT
    GCGTAGGGAACTCAGTTACTTTTGGTCATCCTAATGATGCAGGTGGATGCAGGGAACATTTTCTCAATTT
    TTTTCATTCCCTCAGTGTTCCCATCCTGTGATGGCCGTGTCTCACCTCCATGCCAGGGGCCACACTGGGT
    GTGGAAACTGCAGACAGGCCCTGCCCTCACAGAGCAGCCAGAAAGTCAAAAAAGAGTTCAGGCATGGACA
    TACTTGGACATATGTTTGTGTGGGTGCTCAGAGGCCCCCGGTATCACGAGGGAGGGAGTGGGGTGTGTGT
    GAAGCTGCCCTAGACTAGGAGGTCAAGTGTTCTAGGCTTGGTTCCTCCACTGGATGGACCTCCCTGTTCT
    AACCACTGTAACTGAGCTCTGTTTGAGCTGGAAGGGCTGGAAAGCGTCCACATCTCCGAGATCTCTCTCC
    TTGGCCAAGAGCGCAGTTTGTAAATACTGCACAGAGCCTGTGTCTGTCCATCCTCACTGACCATACCACA
    CCATTGAATGAGAGAGGAGATGCGGGTGAGCCCTGACAAGGCTGGTTCATGCAGACCAGAACCAAACAAC
    TATTCCAGTTGCTAAAGGCCTCCCTTATGTGTCAGGCCATGGGGAAGTGAATGTAACCCAGAGGACACTG
    GGGTACCCACAAGCCCAGGAAAAGGCTTCTGGGGAAGATGGAGATGCCAGAATAGACAGGGGGCGTGGAA
    GACCACCCCTCACCCAGAGTTCCTATTGGCAAAGGCCTCCTTCATGAGGACACTCAGCAATGGCCAGCCC
    TGGGGATCATTTCACACTGCGTGGGCAGAGCCTTAGTGGGGAAAGGATGTTGGGAGGGTCCCTAGACAAG
    CACTTGGGAGATGCTCTAGCGTAAGACTGAGGAACCTGAGCAGGAAGTTGAGTCCACCATGACTTTAGTC
    CCTTGCTCCGTTGCCTTCTGTGTGACTCAGTTTCTACAATGGCAAAGGCGCAGTTGCTCACCAGGCCCCT
    GACTGCCTTCCCTGATCTGTATAGACAGCTGGGAGAAGAGGTAACTCCTCATTCAGGAAGCAGCCACCCA
    ACGGGGCTTGACACTGCAGGAAGAACACCCAGCCGGGTTGCACGAAGCCCACGGGGCTTGTTAGTCAGGC
    CTGTGTTTTCCTTGCAGATGGTCAGGGTCCCCTGCACCATGGTGTCTGCAGCACTTGGATCAGGAACCTG
    AAGCCCCAGGACCCAGTGCCCTGCTTTGTGCGAAGGTAAGCACCCACTCGGGGCCCTTTGGAGCCCCTAG
    CCCCTGGGGATTCTGCAATGCAAGGGAAGGGTCTAAGACTGAGTCTCCTCTCGGCCCATAAAGGTTTAGG
    GGGTAATTCTTTGTCTCCTCGTTTTGCCATCTGGTTACAAGGTGTCGTGGAGTCCATGACACAGATACAG
    TGATGCCTGTGGTGGGATCCAATGAGACTGGCCCAGTGTGGAGAGAGGCCGTTGCCTGGGCTGGGAAGAT
    GAAGATGTGCACAGGTCCCCGCCAGGCGCTTAGGTCCCCAGCCCTATCCTGCATGTGCTCCTAGCCTGGG
    ATATCTGCAGACCAGGGTCTTTCTCCTTCTGTCTTCCTGCAGTGTCAGTGGCTTCCAGCTCCCTGAGGAC
    CCCTCCCAGCCTTGCATCCTCATTGGGCCTGGTACGGGCATTGCTCCCTTCCGAAGTTTCTGGCAGCAGC
    GGCTCCATGACTCCCAGCACAAAGGTACAGCTCTTACTCAATAGGGAGTGTGCCTGTCACTGTGACCGAC
    CTGATCATTACACAGGCACATCAAATTACTACACCATATCCCATAATCAGGTACACAGATGTGGAGTGTT
    TGTGCTCTGCCCACTCCACGTCTCCATGTTTCCTTGTTAATTTTTAAGGTATCTAGAGGAGTGTGTATGT
    CCTGGTACGCATGTGGAGGTCAGAGGTCAACTTGTTTTACTTCTTCCAGGAATCAGATTCAGCTTGTCAG
    TCACGACGGCAAGCACCTCTCCCTGCTGAGCCATCTCATCAGCCCATTTTGCTGGGTTTTAAATGCTTGC
    CTCCTTGCTGGACCATCCATTAATAACACAGAGATTAATAAGCCTGTGTAGGGGTCCCCAGAGTGAAGGG
    GGAATGAGAGTAACAGACTTTAGATGAAGACATTTTCTTTTCATTGGAGAAATAGCTGGGAAGGATGGAC
    AGCTCTGTCTGGAATCCTCCAGATGAGGCTTTTCTGGAAGAAACCCTTAGCAATGTTTGACCTTCAAGAT
    GCCTGAGGCCTCTGCACCTATATTTGATGTAGCATCTGGGTCAGGGGAGATGATTTTCACAAAAGAAGGC
    CACGAGTTTCTGATATTCCACTAAAAGAATGTCCTTAGCATGTTACACACACACACACCTATACATATTT
    GAGACAAAGTCTTGCTATTGTAGCCCTGGATGAACTGAAAATTCCCTATACAGAGGAGGCTGTCCTCAAA
    TTTGCAGGAAATCAATCCTCCTGCTTCTAATTCCCAAGAACTGGCTATAATAATATTTTAAAAGAAGAAG
    TAAGATTAAAATAGAATATGTCAAGTAAAAGAAATAGCAATGTGTAGACATAATCAGAACTGATACTTTT
    TTTTCCTGAACCATGTTTTTTAAGAGTGCCACCATTAGCTATAAAATGGGAATAATGCCAGATCAGAAAC
    CTACCCATTGGGTACCTGGTGCTCACAGGTTGCCCTCTGGTGGCCGTTGTGGTAACTGCAACCTTCTGGA
    TCTTCAAGGGCTCAAAGGAGGCCGCATGAGCTTGGTGTTTGGGTGCCGGCACCCGGAGGAGGACCACCTC
    TATCAGGAAGAAATGCAGGAGATGGTCCGCAAGAGAGTGCTGTTCCAGGTGCACACAGGCTACTCCCGGC
    TGCCCGGCAAACCCAAGGTAAGTGCAGCCACCCAGCAAGCTGGCAGAGCACGCATGATGGCCCCTGCCCA
    GGCTATACAGCCCTTCCACTCCAAGCCTGCAGTGAAGACAGTTGCACAGTGGCAGGGAGAGATGAAGAGC
    ACGATGACAACATCAAGCTCACTGTAGTTTAATCTCCACAGTGCAAACACCTGCAGGTGCCCAGCATCTC
    TGGGACGCAGGGCTGGGCCGGGCTGGCTTTACACTTAGTTCAGTAAGACTGGAAGGTGGAGCTGATTCCC
    CCCAGGGCCCTCAGGTTGGCACTCTGCTCCAGGGGCATGAACAGTGCCTACACACTTTCCATTCAGGACC
    ATGTGAACATTCCCTGAAGACCCCATTTGCATCCTAAAGCTGGACCACTGGTCATCTTAGATGAAAAACA
    AAAACAAACAGACTCCTGACTGACTGCAGGACCTTCTTTATAAATGGAAAGCCTAGGAGCCTATACCTGA
    GCTACAAATCAGAGCTGGGGCTAATACATACTGGGAAGCCCCCTCTTTCCGGGGTGTAGACAGGCAGATC
    TCACAGCCCTGGGCAGAGCTATAAACTTGATGTCTCTAAAGTATGGTGACCTGAGGGAAGCTTTACGGGT
    TCCATTGGCCAGGAGGACAGGATACTTGTCTATCCTGAGCTGAGGTCCTGACTGAAGCCCTCAATGGCCC
    TCTTCCCCTCCCCATGGCCAGTGTGCCTGACCACAGTGACACCAGCATCCAGGGCTGGTCCCACCGTGTC
    CTCTGCTTTTAGGTCTACGTTCAGGACATCCTGCAAAAGCAGCTGGCCAATGAGGTACTCAGCGTGCTCC
    ACGGGGAGCAGGGCCACCTCTACATTTGCGGAGATGTGCGCATGGCTCGGGATGTGGCTACCACATTGAA
    GAAGCTGGTGGCCACCAAGCTGAACTTGAGCGAGGAGCAGGTGGAAGACTATTTCTTCCAGCTCAAGGTA
    TGGGAGCTGGGGTGGGACCCTCCAGAGGGGAGTAGACCAAGGTCACAAGCCTTTGGAACTAGGACCAGGG
    ATGCAGAACTGGGGTGGGGGAGGTGTGTGCCCTGAGATCCTCGGCACCACTGCAGAAACCTGGGGCCGGC
    TGGGCTCCTCAAGCCTGGTCTTTCCATGCCATAGTGGTTGCCAGCTAGCTGAGAAATCAGGCCAGTGTGT
    TCCATTTCTCTATATCTGGGATGAAAGCAGCAGTCTCTGCTCCCCACACTCCTGCCTAAATGCCCATTGA
    GCATAACAGGGCTGTGGACTGTCCCCTACTGCCTATGGGGGACAGACACTTATTTACAATAGGTTGCTGG
    CTAGGTGCCCTCATTTCTCACAGGAAGGACTCTTGGTAGCAGGGTCTGCCTTGTGCCAGGTGCTCACCAA
    TGATTGCCCCGTGGTGACCTTTCCTTCTGCCCTTCCAAGGGCTTTATGACGGAAGAATGACTGTTCCATC
    ATTTTGCAGATGGAAGGGGCCCTGGTGCCCTAACAGCGAATGTGAGAGCCCAGACACATGGCCCCTCTCT
    CCTTTTCCCTCTCTCTGTCTCTCTGGTTCCTAAACCTGCCTTTAAGGCTCGAAGTTATGCTCCCCATTTA
    TATTAAACATCTACTATGTGTTAGAGGTAAGAAGCAGTCAACAAAACAGGTGAAAATGTTGTCAATATCA
    CGATAGATCACAGAGCACACCTGTATCGCCAGCACTGGGGAGGTGGAGGCCCAGGGGTCAAGAGTTCAGA
    TTGTCCCTGGGTTTCATAGTCAGTCCAAGGCTGGACTGGGCTATATGAGATCCTATTTCGAAAGTACAAA
    AGCAAAAGACCTACCTAAACGTAGCTTACATTCTGGCAGAGTTGATCAAATCAAGCACATAGGGTGCGCA
    CTGCTTTGGGTAAGACGCAGAACAGCAGCTCAGAGCTGGGGTGTTTGTGGTTTAATGAGGTTTGGGGAAG
    GGGTCCCCGGGAGGTGACATTTGAGAAAAATACCTGAAGAAGGAAGAAGCTTTGTGCACACTGGAGACAG
    AGGTCCAGACTATTCTGTGGGGCAGCAACATGTTCAAAGGTCCTGGGGTGTGGACTATTCTGAAACCTGT
    GATGACCAGCAAGGGTATCATGGGTACTTCCCAAAATGTGTTCAGAGCTTGCCAGATGTGTTCATGGATG
    TTAGACATCATTAACAATGGGCTGTAAGGGCAGAGGCAGTGCACACCTGTTAGTCCTAAGGCTTTGGGGA
    AGCTGAGGCAGGAGAGTTGTAAGTTCAAGTCAGTATGGGCTACCTTAGGCTGCAAGAATGCAGAGGACAT
    ACCTGTAACATCAGCTCCAGGGGATCTGATCCCTCATATACAGGTACATGTATACACACACACACACACA
    CACACACACTTGAAAATAAAACTGTAGCCAGACATGGAGGCATGTGCACACCTTTAACTCTAGCACTCAG
    AGGCAGGAAATAAAATCCTTTCTTTTTTATTTATTTTAATTTAAAAAGAGAAAATTAGTTCCAGGGGTAT
    AGCTTAGGCCTTGCCTAGCCTGGTATATAAAAGGCCCCAGGTTTGATTTCTAGTACCAAGGGAAAAAAAG
    ATTCCAAGGCCAGACATTTGGTACACACGTGCGCACCCACAAAGAGCAACAGGTGTCTGCTGTAAGATTT
    CTCTCGGAGCCCTCAGCCTGCTGCTGATTTCAAGGCCTGAAGAATGGGAGGCACACACTGCCTGCCCTGC
    ACATCCTGATCTCAAAGCTCACATCCGATGTGGGATGAGCACTAGACAGGTAGTGGGACCGACATCCATC
    CATGAAGTCTGGGAAGAGGCTGAGGTGGGCCACACATGGGCAGAGGAGAAACCAAGCATGGGTGTAATGG
    CAGCAATCCAGACAAGGGGGTAGACACAGGGCTAGCGAGCGCCTGTGTCACCAGGAAGCTGTGGCAGCCC
    TAGGGTTTTGTCCAAGCCTAAACATGATAACTGAGAGGTGGCTGGGCCAGCAGGGTGACACTGTCACCTT
    TGTTTCCCCCTCACAGAGCCAGAAACGTTATCATGAAGATATCTTCGGTGCAGTCTTTTCCTATGGGGCA
    AAAAAGGGCAGCGCCTTGGAGGAGCCCAAAGCCACGAGGCTCTGACAGCCCAGAGTTCCAGCTTCTGGCA
    CTGAGTAAAGATAATGGTGAGGGGCTTGGGGAGACAGCGAAATGCAATCCCCCCCAAGCCCCTCATGTCA
    TTCCCCCCTCCTCCACCCTACCAAGTAGTATTGTACTATTGTGGACTACTAAATCTCTCTCCTCTCCTCC
    CTCCCCTCTCTCCCTTTCCTCCCTTCTTCTCCACTCCCCAGCTCCCTCCTTCTCCTTCTCCTCCTTTGCC
    TCTCACTCTTCCTTGGAGCTGAGAGCAGAGAAAAACTCAACCTCCTGACTGAAGCACTTTGGGTGACCAC
    CAGGAGGCACCATGCCGCCGCTCTAATACTTAGCTGCACTATGTACAGATATTTATACTTCATATT
  • Protein Sequence : Show Sequence
    >gi|6754872|ref|NP_035057.1| nitric oxide synthase, inducible [Mus musculus]
    MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLH
    VTSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEF
    INQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARN
    CSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEF
    TQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANML
    LEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSF
    QKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPW
    KTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFN
    TKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCA
    FAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEP
    QQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIF
    PGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKL
    ARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDH
    TPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGI
    APFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYV
    QDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHED
    IFGAVFSYGAKKGSALEEPKATRL
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis guaB mutant vaccine , LVS
15. TNF-alpha
  • Gene Name : TNF-alpha
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 21926
  • NCBI Protein GI : 7305585
  • Genbank Accession : AB039224
  • Protein Accession : NP_038721
  • Taxonomy ID : 10090
  • Chromosome No : 17
  • Gene Starting Position : 35199389
  • Gene Ending Position : 35201995
  • Gene Strand (Orientation) : -
  • Protein Name : tumor necrosis factor
  • Protein pI : 4.75
  • Protein Weight : 24010.47
  • Protein Length : 235
  • Protein Note : Also known as DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434
  • DNA Sequence : Show Sequence
    >gi|372099093:35199389-35201995 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
    CTTTATTTCTCTCAATGACCCGTAGGGCGATTACAGTCACGGCTCCCGTGGGGAGCAGAGGTTCAGTGAT
    GTAGCGACAGCCTGGTCACCAAATCAGCGTTATTAAGACAATTGGGTTAGATAAATATTTTGTTTTAAAC
    ATAAGCAAAAGAGGAGGCAACAAGGTAGAGAGGCCAGGTGGGGACAGCTCAGCTCCGTTTTCACAGAAAA
    CATGTCTGTCTGAAGACAGCTTCCCACACTGGGTCCTCCAGGACACCCCGGCCTTCCAAATAAATACATT
    CATAAGCAAATAAATAAATAATAAATAAATAATAAATAATAAGTGCAAATATAAATAGAGGGGGGCTGGC
    TCTGTGAGGAAGGCTGTGCATTGCACCTCAGGGAAGAATCTGGAAAGGTCTGAAGGTAGGAAGGCCTGAG
    ATCTTATCCAGCCTCATTCTGAGACAGAGGCAACCTGACCACTCTCCCTTTGCAGAACTCAGGAATGGAC
    ATTCGAGGCTCCAGTGAATTCGGAAAGCCCATTTGAGTCCTTGATGGTGGTGCATGAGAGGCCCACAGTC
    CAGGTCACTGTCCCAGCATCTTGTGTTTCTGAGTAGTTGTTGAAAGCTCTGAGCACAGAGTTGGACCCTG
    AGCCATAATCCCCTTTCTAAGTTAGAAGGATACAGACTGGGGGCTCTGAGGAGTAGACAATAAAGGGGTC
    AGAGTAAAGGGGTCAGAGTGGGGGCTGGGTAGAGAATGGATGAACACCCATTCCCTTCACAGAGCAATGA
    CTCCAAAGTAGACCTGCCCGGACTCCGCAAAGTCTAAGTACTTGGGCAGATTGACCTCAGCGCTGAGTTG
    GTCCCCCTTCTCCAGCTGGAAGACTCCTCCCAGGTATATGGGCTCATACCAGGGTTTGAGCTCAGCCCCC
    TCAGGGGTGTCCTTGGGGCAGGGGCTCTTGACGGCAGAGAGGAGGTTGACTTTCTCCTGGTATGAGATAG
    CAAATCGGCTGACGGTGTGGGTGAGGAGCACGTAGTCGGGGCAGCCTTGTCCCTTGAAGAGAACCTGGGA
    GTAGACAAGGTACAACCCATCGGCTGGCACCACTAGTTGGTTGTCTTTGAGATCCATGCCGTTGGCCAGG
    AGGGCGTTGGCGCGCTGGCTCAGCCACTCCAGCTGCTCCTCCACTTGGTGGTTTGCTGAGGGGGGGGGGG
    AGGATTGAGTCAGTGTCACCCTCTTAGTTCACACTCCACATCCTGAGCCTCAGCAGCTACCCACACTTCA
    CTTCCGGTTCCTGCACCCTCTGTCTTTCCACATCCCATTGGCTATGAGGTCCCGGGTGGCCCCCTGATGC
    CTTGCTTTTGAGTCACTGCTCTGACTCTCACGTGCTGTCTCTAAGAGCTCTGTCTTTTCTCAGCCTGGCT
    CGACACCCCTCAACCCGCCCCCCAAAATCATGCCCCTTCATTCTCAAGGCACATGTAAAGAAATCTTACC
    TACGACGTGGGCTACAGGCTTGTCACTCGAATTTTGAGAAGATGATCCTGGAGGGGAAGAGACAAAGGCA
    AGGATGAGCCTTTTAGGCTTCCCAGCAAGCATCTATGCACTTAGACCCCTTTCCTCCCAAACCAAAGCTT
    TAAGTTCTCCCCCCACCCCATCTCATCCCATGCCTAACTGCCCTTCCTCCATCTTAAATTAAGAGAGAGG
    TGTGGGAACACTTACTGAGTGTGAGGGTCTGGGCCATAGAACTGATGAGAGGGAGGCCATTTGGGAACTT
    CTGTGTAGGAAAAGGAGGTTAGTTAAGACAGACTCACCCCAAAGGAGAAGCCTCCCGGCTGATTGCCCCG
    CTTACAGTTCCTCTTTGCCCCACCCCACCCCCCAGCTTTGTGTTTTTCTTCTTCATTCATTCATCTGTCC
    AACCCACGGCTTCTTTCTGCGGTGCCCTCTGTGCTTGATCTCCCGTTATCTCCCCTTCATCTTCCTCCTT
    ATCTCTCATGCCTCTCTCATTTCTGTCTCTGAGTTTTATCTCTTGCTTATCCCCTCTTCCCCTGGCCACA
    TCTTTCCAGATCTCTCCACGTGTGAACACACTTGTTCGTTCATTCATCTCTCTGTGCATCCGACGAAGGA
    TGTTTAGTCAGCTGGACGCATGGGTCCGAGGTCCTGACTCTGTCCCCTCCACACTCTCCTCCACCTTGCC
    CTGCCCATTAGCCCACTTCTTTCCCTCACACTGTCCTTCTTGCCCTCCTAACCCGTTTTGCTTGTGAGCG
    AGAATAAGGGTTGCCCAGACACTCACCTCATCCCTTTGGGGACCGATCACCCCGAAGTTCAGTAGACAGA
    AGAGCGTGGTGGCCCCTGCCACAAGCAGGAATGAGAAGAGGCTGAGACATAGGCACCGCCTGGAGTTCTG
    GAAGCCCCCCATCTTTTGGGGGAGTGCCTCTTCTGCCAGTTCCACGTCGCGGATCATGCTTTCTGTGCTC
    ATGGTGTCTTTTCTGGAGGGAGATGTGGCGCCTTGGGCCAGTGAGTGAAAGGGACAGAACCTGCCTGGTT
    GGCTGCTTGCTTTTCTGGGAGCTATTTCCAAGATGTTCTGGAGTTTCTGTTCTCCCTCCTGGCTAGTCCC
    TTGCTGTCCTCGCTGAG
  • Protein Sequence : Show Sequence
    >gi|7305585|ref|NP_038721.1| tumor necrosis factor [Mus musculus]
    MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPL
    ISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQ
    VLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQL
    SAEVNLPKYLDFAESGQVYFGVIAL
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Francisella tularensis FTL0552 mutant vaccine , Francisella tularensis guaB mutant vaccine
IV. Vaccine Information
1. DeltaFTT0918
a. Vaccine Ontology ID:
VO_0011346
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Antigen
DeltaFTT0918 is an attenuated SCHU S4 strain with mutation of the gene FTT0918 (Twine et al., 2005).
e. Immunization Route
Intradermal injection (i.d.)
2. F. tularensis FopB Protein Vaccine
a. Vaccine Ontology ID:
VO_0004034
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
.5 μg recombinant FopB protein
e. Gene Engineering of FopB
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant: CpG DNA vaccine adjuvant
  • VO ID: VO_0001237
  • Description: 10 μg of CpG oligodeoxynucleotide (5′-TCCATGACGTTCCTGACGTT-3′; Operon, Huntsville, AL)
g. Immunization Route
intranasal immunization
h. Mouse Response
  • Host Strain: C57BL/6 and HLA-DR4 tg
  • Vaccination Protocol: mice were anesthetized i.n. with 3% isoflurane by use of a rodent anesthesia system and were immunized i.n. on day 0 with 7.5 μg rFopB in 25 μl of sterile PBS. This was accompanied by immunization with 10 μg of CpG oligodeoxynucleotide on days −1, 0, and +1. Mice were boosted once on day 14 with the same amount of antigen (Yu et al., 2010).
  • Challenge Protocol: Mice were challenged i.n. with approximately 3.5 × 104 CFU (∼5 50% lethal doses [LD50s]) of LVS on day 30 (Yu et al., 2010).
  • Efficacy: Intranasal vaccination of mice with rFopB conferred 50% protection (P < 0.05) against an otherwise lethal pulmonary challenge of 5 LD50s of LVS (3.5 × 104 CFU) in both C57BL/6 and HLA-DR4 tg mice compared with the protection conferred to LVS-challenged mock-vaccinated mice, all of which succumbed to the infection by day 10 (Yu et al., 2010).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: Spleens were collected at 14 days postvaccination, and single cells were prepared and assayed for FopB- and LVS-induced IFN-γ production. Differences in IFN-γ stimulation between rFopB- and mock-vaccinated mice were significant (Yu et al., 2010).
    • Detailed Gene Information: Click here.
3. F. tularensis GroEL protein vaccine
a. Vaccine Ontology ID:
VO_0011446
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
F. tularensis chaperonin GroEL (Hsp60)
e. Gene Engineering of GroEL
  • Type: Recombinant protein preparation
  • Description: Hsp60 was identified by Western blotting with a monoclonal antibody to GroEL (Sigma-Aldrich, Poole, United Kingdom). Hsp60 was excised from large-format unstained gels and electroeluted into 4× Laemmli buffer (0.1 M Tris-HCl [pH 7.3], 0.768 M glycine, 0.4% [wt/vol] SDS) by using the Hoefer gel eluter (Amersham Pharmacia, Buckinghamshire, United Kingdom) at 70 V for 2 h, following the manufacturer's instructions. The eluted product was pooled and purified by dilution (more than 20×) in ammonium bicarbonate (3.95 g/liter) and SDS (1 g/liter) and centrifuged over a dialysis membrane (10,000-molecular-weight cutoff; Vivascience, Lincoln, United Kingdom). The protein was then diluted (20×) in sterile water and centrifuged further, until significant concentration was achieved (Hartley et al., 2004).
  • Detailed Gene Information: Click here.
f. Adjuvant: IL-12 vacince adjuvant
g. Immunization Route
Intraperitoneal injection (i.p.)
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Groups of BALB/c mice 6 to 8 weeks old (Charles River International, Margate, United Kingdom) were immunized i.p. on days 0 and 14 with 25 μg of Hsp60/100 μl of saline with or without 0.5 μg of murine IL-12. Control groups were immunized with either IL-12 only or 100 μl of saline containing eluted protein-free gel. IL-12 was kindly provided by Roche Pharmaceuticals. Blood samples were obtained from each mouse by tail vein bleeding immediately prior to challenge (day 28). The serum was separated by microcentrifugation and stored at −20°C until analysis. Groups of mice were either challenged or culled to provide spleen cells a further 14 days later (Hartley et al., 2004).
  • Challenge Protocol: F. tularensis LVS, F. tularensis subsp. holarctica HN63, and F. tularensis subsp. tularensis Schu S4 were used for challenges. The strains were harvested into PBS after growth on BCGA for 24 h at 37°C and diluted to a given optical density. Confirmation of the dose was achieved by plating out a serial dilution. Groups of mice were challenged on day 28 with either 103 or 104 MLD of LVS i.p. or 100 MLD of HN63 or 10 MLD of Schu4 subcutaneously as separate challenges. Mice were monitored for 14 days, and survival to a humane end point was recorded (Hartley et al., 2004).
  • Efficacy: Researchers have shown that mice that had been immunized with purified heat shock protein 60 (Hsp60, groEL) isolated from Francisella tularensis were protected against a subsequent challenge with some strains of the bacterium (Hartley et al., 2004).
4. F. tularensis vaccine LVS LPS
a. Vaccine Ontology ID:
VO_0000308
b. Type:
Subunit vaccine
c. Antigen
Francisella tularensis live vaccine strain (LVS) lipopolysaccharide (LPS) (Dreisbach et al., 2000). The chemical composition of the lipid A moiety of LVS LPS is quite different from that of enteric LPS. The LVS LPS lacks a functional lipid A. This apparently confers a survival advantage on the bacterium, since its LPS does not participate in induction of nitric oxide production that might limit its intracellular growth. Further, since other data indicate that LVS LPS is unable to block macrophage stimulation by functional LPS, the structure must be distinct enough to not permit recognition as an antagonist for traditional LPSs. The ability of LVS LPS to stimulate protection in C3H/HeJ mice, which are defective in the ability to recognize and respond to enteric LPS at least in part due to a point mutation in Toll-like receptor 4, suggests that LVS LPS is recognized through receptors other than Tlr4 (Dreisbach et al., 2000).
d. Preparation
Francisella tularensis live vaccine strain (LVS) LPS is purified from whole F. tularensis LVS bacteria. A culture of F. tularensis LVS is grown in 4-L flasks at 37°C for 48 h in Trypticase soy broth with cysteine. The cultures are centrifuged for 15 min, washed 3 times in PBS, once in methanol, and once in acetone, and then lyophilized. The LPS is then extracted. After the crude LPS is treated with DNase, RNase, and proteinase K, the pellet is harvested by centrifugation 3 times for 12 h. LPS preparations are then reconstituted in endotoxin-free PBS and stored at 4°C (Dreisbach et al., 2000).
e. Virulence
No traditional endotoxin has been associated with virulent F. tularensis. More recent reports indicated that purified LVS LPS was not endotoxic in d-galactosamine-sensitized mice and failed to activate Limulus amoebocyte lysate. LVS LPS also failed to stimulate human monocytes or peripheral blood lymphocytes to proliferate, produce TNF-α, or produce IL-1. Mouse peritoneal exudate macrophages treated with LVS LPS did not produce TNF-α or nitric oxide, and there was no increase in surface immunoglobulin expression by a mouse pre-B-cell line in response to LVS LPS. The only reported biological activity of LVS LPS is activation of complement (Dreisbach et al., 2000). LPS purified from F. tularensis LVS did not activate murine B cells for proliferation or polyclonal immunoglobulin secretion, nor did it activate murine splenocytes for secretion of interleukin-4 (IL-4), IL-6, IL-12, or IFN-gamma. However, in vivo experiments have suggested that LVS LPS contributes to the virulence of Francisella, in that LPS-defective Lpsd C3H/HeJ mice are reported to be more susceptible to LVS infection than Lpsn C3H/HeN (Dreisbach et al., 2000).
f. Mouse Response
  • Host Strain: BALB/cByJ, C57BL/6J, BALB/c.scid
  • Vaccination Protocol: Normal mouse serum (NMS) was obtained by bleeding normal BALB/cByJ mice from the lateral tail vein and pooling the resulting serum. Normal BALB/cByJ mice were then immunized with 10 µg of LPS intradermally (i.d.), and immune mouse serum (IMS) was obtained 3 or 30 days later. BALB/cByJ mice were given 0.5 ml of a 1:4 dilution of these sera i.p. 1 day before challenge with 103 LVS bacteria i.p. (Dreisbach et al., 2000).
  • Persistence: T-cell-deficient BALB/c.nu/nu mice treated with LVS LPS survived lethal challenge for 2 to 3 weeks longer than control (PBS-treated) mice; these mice eventually succumbed to challenge after ~3-4 weeks (Dreisbach et al., 2000).
  • Immune Response: LPS purified from F. tularensis LVS lacks the ability to nonspecifically activate murine B cells for proliferation or polyclonal immunoglobulin secretion. It is also unable to activate murine or human macrophages (Dreisbach et al., 2000). It does not stimulate murine splenocytes to secrete IL-12, IL-6, IL-4, or IFN-γ (Dreisbach et al., 2000). LVS LPS is unable to activate murine or human macrophages. LVS LPS does stimulate specific antibody production and in purified form is a weak immunogen that induces primarily an IgM response. When recognized by the murine immune system as a component of the bacterium, however, the resulting specific antibody response is vigorous and is characterized by the production of large amounts of IgG, particularly IgG2a. Despite the apparent absence of nonspecific immunostimulatory activity and minimal specific antibody production, however, treatment of mice with surprisingly small amounts of LVS LPS stimulates very strong B-cell-dependent protection against lethal LVS challenge within 2 to 3 days (Dreisbach et al., 2000).
  • Side Effects: None were noted (Dreisbach et al., 2000).
  • Challenge Protocol: BALB/cByJ mice were given various doses of LVS LPS i.d. on day 0 and challenged 3 days later with lethal doses of LVS, either 103 (1,000 LD50) or 104 (10,000 LD50) bacteria (Dreisbach et al., 2000).
  • Efficacy: Very strong protection against lethal LVS infection was demonstrated. Mice given doses as low as 0.1 ng of LVS LPS i.d. survived lethal LVS challenge, depending on the strength of the challenge given. Mice given 100 ng of LVS LPS i.d. survived LVS challenge doses approaching 106 bacteria (1,000,000 LD50). Further, in three experiments, 15 of 15 mice challenged with 104 LVS bacteria i.p. 3, 10, or 35 days after treatment with 100 ng of LVS LPS i.d. survived; however, in the same experiments none of 15 mice treated with 100 ng of LVS LPS i.d. and challenged 3, 10, or 35 days with 20 LD50 of L. monocytogenes survived. Early protection also could not be demonstrated using other types of LPS, in that mice given 100 ng of E. coli O55 LPS, Salmonella serovar Typhimurium LPS, or E. coli K235 LPS i.d. on day 0 and challenged with 103 LVS bacteria i.p. on day 3 did not survive.
5. F. tularensis vaccine rLm/iglC
a. Vaccine Ontology ID:
VO_0011447
b. Type:
Recombinant vector vaccine
c. Status:
Research
d. Antigen
F. tularensis intracellular growth locus, subunit C (iglC)
e. Gene Engineering of IglC1
  • Type: DNA vaccine construction
  • Description: Constructed recombinant L. monocytogenes (rLm) vaccines stably expressing seven F. tularensis proteins including IglC (rLm/iglC) (Jia et al., 2009).
  • Detailed Gene Information: Click here.
f. Gene Engineering of IglC2
  • Type: Recombinant vector construction
  • Description: Constructed recombinant L. monocytogenes (rLm) vaccines stably expressing seven F. tularensis proteins including IglC (rLm/iglC) (Jia et al., 2009).
  • Detailed Gene Information: Click here.
g. Vector:
rLmΔActA
h. Immunization Route
Intradermal injection (i.d.)
i. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Six groups of 8 mice were sham-immunized or immunized with LVS or rLm vaccines at weeks 0 and 4 (Jia et al., 2009).
  • Challenge Protocol: At week 8, mice were anesthetized with Ketamine/Xylazine, challenged i.n. with 4400 CFU of LVS, and monitored for survival for up to 3 weeks after challenge. Intranasal challenge with 65× LD50 of LVS. Groups of mice were sham-immunized or immunized i.d. twice at weeks 0 and 4 with 5 × 104 CFU LVS or 1 × 106 CFU of rLmΔactA (vector), rLm/iglC, rLm/katG or rLm/iglC plus rLm/katG (Jia et al., 2009).
  • Efficacy: Mice immunized with rLm/iglC were protected against lethal challenge with F. tularensis LVS administered by the intranasal route, a route chosen to mimic airborne infection, and, most importantly, against aerosol challenge with the highly virulent Type A F. tularensis SchuS4 strain (Jia et al., 2009).
6. F. tularensis vaccine X4072(pTUIA-15)
a. Vaccine Ontology ID:
VO_0011548
b. Type:
Recombinant vector vaccine
c. Status:
Research
d. Antigen
F. tularensis 17 kDa major membrane protein (TUL4) precursor
e. Gene Engineering of TUL4
  • Type: Recombinant vector construction
  • Description: A 1.0-kb EcoRV-SphI DNA fragment was prepared by the technique of Birnboim and Doly (6) from the plasmid pTUL4-9 (39), a derivative of pUC18. The fragment contains the gene encoding the T-cell-reactive 17-kDa protein TUL4 but contains no other expressed open reading frames (40). The isolated 1.0-kb fragment was ligated to the Asd+ plasmid pYA248 (27) after the plasmid had been cleaved with EcoRI and SphI and after the EcoRI site had been made blunt by using the Klenow fragment. The translation initiation codon of pYA248 was followed by a short sequence of 36 putatively encoding nucleotides of the Francisella DNA. The recombinant plasmid, denoted pTULA-15, was transformed (16, 20) into S. typhimunium x3730. By using a lysate of the X3730 recombinant, transduction of phage P22 HT int into X4072 was performed according to standard methods (12, 37). The presence of TUIA in lysates from transduced strains was confirmed by Western blot analysis, and one of the strains, denoted S. typhimurium X4072(pTUL4-15), was selected for the experiments (Sjöstedt et al., 1992).
  • Detailed Gene Information: Click here.
f. Vector:
S. typhimurium X4072
g. Immunization Route
Intraperitoneal injection (i.p.)
h. Mouse Response
  • Host Strain: C57BL/10
  • Vaccination Protocol: Mice were immunized twice, 4 weeks apart, by the intraperitoneal injection of 100 ,ll of PBS containing 5 x 103 (5 x 102 for the more susceptible mouse strain C57BL/10) organisms of S. typhimurium X4072 or S. typhimunum X4072(pTUIA-15) (Sjöstedt et al., 1992).
  • Challenge Protocol: Three months after the completion of immunization, mice were intravenously challenged with F. tularensis LVS at a dose of 160 to 230 bacteria. At various intervals thereafter, mice were sacrificed for the enumeration of bacteria in the liver and spleen. The CFU per organ were recorded (Sjöstedt et al., 1992).
  • Efficacy: A 17-kDa lipoprotein, TUL4, of the facultative intracellular bacterium Francisella tularensis is one of several membrane proteins that induce an in vitro response in T cells from F. tularensis-primed humans. When mice were immunized with S. typhimurium chi 4072(pTUL4-15), some animals showed an antibody response and a T-cell response to TUL4. The present study demonstrated that the 17-kDa lipoprotein TUL4 of F. tularensis is involved in a protective immunity to tularemia (Sjöstedt et al., 1992).
7. Francisella tularensis capB mutant vaccine
a. Vaccine Ontology ID:
VO_0002840
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of capB
  • Type: Gene mutation
  • Description: This capB mutant is from Francisella tularensis (Jia et al., 2010).
  • Detailed Gene Information: Click here.
f. Immunization Route
intranasal immunization
g. Mouse Response
  • Persistence: A capB mutant is attenuated in mice (Jia et al., 2010).
  • Efficacy: A capB mutant induces significant protection in mice from challenge with wild type F. tularensis (Jia et al., 2010).
  • Host Gene Response of IgG
    • Gene Response: Following i.n. immunization, LVS ΔcapB induced significantly higher levels of serum IgG, IgG2a, and IgM antibodies in mice than LVS by 7 days post immunization (Jia et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighv1-9
    • Gene Response: Following i.n. immunization, LVS ΔcapB induced significantly higher levels of serum IgG, IgG2a, and IgM antibodies in mice than LVS by 7 days post immunization (Jia et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgM
    • Gene Response: Following i.n. immunization, LVS ΔcapB induced significantly higher levels of serum IgG, IgG2a, and IgM antibodies in mice than LVS by 7 days post immunization (Jia et al., 2010).
    • Detailed Gene Information: Click here.
8. Francisella tularensis clpB mutant vaccine
a. Vaccine Ontology ID:
VO_0002841
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of clpB
f. Immunization Route
Intradermal injection (i.d.)
g. Mouse Response
  • Persistence: A clpB mutant is attenuated in mice (Conlan et al., 2010).
  • Efficacy: A clpB mutant induces significant protection in mice from challenge with wild type F. tularensis (Conlan et al., 2010).
9. Francisella tularensis FTL0552 mutant vaccine
a. Vaccine Ontology ID:
VO_0002842
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of FTL0552
f. Immunization Route
intranasal immunization
g. Mouse Response
  • Persistence: A FTL0552 mutant is attenuated in mice (Sammons-Jackson et al., 2008).
  • Efficacy: A FTL0552 mutant provided partial protection in mice from challenge with wild type Francisella tularensis SchuS4 (Sammons-Jackson et al., 2008).
  • Host Gene Response of Ccl2
    • Gene Response: LVS induced a significantly higher MCP-1 (Ccl2) response in mice lung homogenates as compared to FTL0552 mice beginning day 3 after infection (Sammons-Jackson et al., 2008).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: LVS induced a significantly higher IFN-gamma response in mice lung homogenates as compared to FTL0552 mice beginning day 5 after infection (Sammons-Jackson et al., 2008).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-6
    • Gene Response: LVS induced a significantly higher IL-6 response in mice lung homogenates as compared to FTL0552 mice beginning day 5 after infection (Sammons-Jackson et al., 2008).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Il12b
    • Gene Response: An FTL0552 mutant induced significantly elevated levels of IL-12 in C57BL/6 mice lung homogenates by day 7 post infection compared to LVS (Sammons-Jackson et al., 2008).
    • Detailed Gene Information: Click here.
  • Host Gene Response of TNF-alpha
    • Gene Response: LVS induced a significantly higher TNF-alpha response in mice lung homogenates as compared to FTL0552 mice beginning day 5 after infection (Sammons-Jackson et al., 2008).
    • Detailed Gene Information: Click here.
10. Francisella tularensis FTT0918 mutant vaccine
a. Vaccine Ontology ID:
VO_0002843
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of FTT0918
  • Type: Gene mutation
  • Description: This FTT0918 mutant is from Francisella tularensis (Twine et al., 2005).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intradermal injection (i.d.)
g. Mouse Response
  • Persistence: A FTT0918 mutant showed significantly reduced virulence in mice (Twine et al., 2005).
  • Efficacy: A FTT0918 provided protection in mice from challenge with wild type F. tularensis 2 months after immunization (Twine et al., 2005).
11. Francisella tularensis galU mutant vaccine
a. Vaccine Ontology ID:
VO_0002844
b. Type:
Live, attenuated vaccine
c. Status:
Licensed
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of galU
f. Immunization Route
intranasal immunization
g. Mouse Response
  • Persistence: A galU mutant is attenuated in mice, inducing only transient weight loss (Jayakar et al., 2011).
  • Efficacy: A galU mutant induces protection in mice from challenge with wild type F. tularensis, inducing only transient weight loss (Jayakar et al., 2011).
  • Host Gene Response of IL-1b
    • Gene Response: Twenty four hours after infection of a mouse macrophage-like cell line (THP-1) or BMDCs with the galU mutant, the amount of IL-1β released into culture supernatants was significantly higher than was observed following infection with WT FT (Jayakar et al., 2011).
    • Detailed Gene Information: Click here.
12. Francisella tularensis guaA mutant vaccine
a. Vaccine Ontology ID:
VO_0002845
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of guaA
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
13. Francisella tularensis guaB mutant vaccine
a. Vaccine Ontology ID:
VO_0002846
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of guaB
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: A guaB mutant is attenuated in mice (Santiago et al., 2009).
  • Efficacy: A guaB mutant induces significant protection in mice from challenge with wild type F. tularensis (Santiago et al., 2009).
  • Host Gene Response of Ccl5
    • Gene Response: RANTES (Ccl5) and iNOS were induced after 4 hours of infection of the mutant in mouse macrophages and were significantly higher than media controls. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Cxcl1
    • Gene Response: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC (i.e., Cxcl1), and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-1b
    • Gene Response: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC, and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Il12a
    • Gene Response: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC, and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Il12b
    • Gene Response: IL-12 p40 mRNA was induced later in mouse macrophages, but was expressed for a longer period of time than other expressed cytokines. Levels were similar to or greater than levels induced by WT LVS and were significantly greater than media controls (Santiago et al., 2009).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Nos2
    • Gene Response: RANTES and iNOS were induced after 4 hours of infection of the mutant in mouse macrophages and were significantly higher than media controls. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click here.
  • Host Gene Response of TNF-alpha
    • Gene Response: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC, and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click here.
14. Francisella tularensis IglB mutant vaccine
a. Product Name:
KKF235
b. Vaccine Ontology ID:
VO_0002847
c. Type:
Live, attenuated vaccine
d. Status:
Research
e. Host Species as Laboratory Animal Model:
Mouse
f. Gene Engineering of IglB
  • Type: Gene mutation
  • Description: This IgIB mutant is from Francisella tularensis (Yu et al., 2010).
  • Detailed Gene Information: Click here.
g. Immunization Route
Oral immunization
h. Mouse Response
  • Persistence: An IglB mutant is attenuated in mice (Yu et al., 2010).
  • Efficacy: An IglB mutant induces significant protection in mice from challenge with wild type F. tualrensis U112, LVS, and SchuS4 (Yu et al., 2010).
15. Francisella tularensis purF mutant vaccine
a. Vaccine Ontology ID:
VO_0002850
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of purF
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: A purF mutant is attenuated in mice (Quarry et al., 2007).
  • Efficacy: A purF mutant induces significant protection from challenge with wild type F. tularensis (Quarry et al., 2007).
16. Francisella tularensis purMCD mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of PurC/PurD
e. Gene Engineering of purM
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: A purMCD mutant is attenuated in mice (Pechous et al., 2006).
  • Efficacy: A purMCD mutant induces significant protection in mice from challenge with wild type F. tularensis (Pechous et al., 2006).
17. Francisella tularensis sodC mutant vaccine
a. Vaccine Ontology ID:
VO_0002853
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of sodC
f. Immunization Route
intranasal immunization
g. Mouse Response
18. Francisella tularensis wbtA mutant vaccine
a. Vaccine Ontology ID:
VO_0002854
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of wbtA
f. Immunization Route
Intradermal injection (i.d.)
g. Mouse Response
  • Persistence: A wbtA mutant is attenuated in mice (Sebastian et al., 2007).
  • Efficacy: A wbtA mutant induces significant protection in mice from challenge with wild type F. tularensis (Sebastian et al., 2007).
  • Host Gene Response of Ighv1-9
    • Gene Response: The titer of immunoglobulin G (IgG) antibodies directed toward the surface-exposed protein antigens in anti-LVS wbtA antisera was determined to be 8,000 + 1,849, fourfold higher than the titer for LVS-immunized mice. The IgG2a subclass predominated among the IgG subtypes analyzed (Sebastian et al., 2007).
    • Detailed Gene Information: Click here.
19. Francisella tularensis wbtI mutant vaccine
a. Vaccine Ontology ID:
VO_0002855
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of WbtI
  • Type: Gene mutation
  • Description: This wbtI mutant is from Francisella tularensis (Li et al., 2007).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: .A wbtI mutant is attenuated in mice (Li et al., 2007)
  • Efficacy: A wbtI mutant induces protection in mice, especially in higher doses, from challenge with wild type F. tularensis (Li et al., 2007).
20. FSC043
a. Vaccine Ontology ID:
VO_0000339
b. Type:
Live Attenuated
c. Preparation
Strain FSC043 is a spontaneous mutant of the SCHU S4 strain (Twine et al., 2005).
d. Virulence
Attenuated (Twine et al., 2005).
e. Description
FSC043 is a spontaneous mutant of strain SCHU S4 of Francisella tularensis (Twine et al., 2005).
f. Mouse Response
  • Host Strain: BALB/c
  • Persistence: To examine the relative virulence of LVS and FSC043, BALB/c mice were challenged i.d. with CFU of one or other strain and then killed on day 4 of infection, and bacterial burdens in the skin, liver, and spleen were determined. By this time, LVS was present at 100-fold-higher levels than FSC043 in the skin and liver and 20-fold-higher levels in the spleen. The BALB/c mice that were intradermally inoculated LVS showed overt signs of infection before recovering, whereas the mice inoculated with FSC043 remained healthy. When inoculated intravenously, FSC043 grew less well than virulent type A or B strains or LVS in the livers, spleens, and lungs of mice (Twine et al., 2005).
  • Side Effects: Destruction of skin and inflammation was observed at the site of inoculation of FSC043 (Twine et al., 2005).
  • Challenge Protocol: FSC033 was used as the challenge strain to assess the efficacy of the various live vaccines employed. For aerosol exposure, thawed bacteria were diluted in Mueller-Hinton broth; for intradermal inoculations, stocks of the strains were diluted in sterile saline. Intradermal inocula were injected into a fold of skin in the shaved mid-belly, and the blister that formed was circumscribed with indelible marker (Twine et al., 2005).
  • Efficacy: All immunized mice survived and were challenged 77 days later intradermally or by aerosol with type A strain FSC033. Immunization of BALB/c mice with LVS leads to excellent protection against intradermal challenge but only weak protection against low-dose aerosol challenge. The FSC043 immunization afforded a similar degree of protection to LVS against intradermal challenge and somewhat better protection against aerosol challenge (Twine et al., 2005).
21. J5dLPS/OMP
a. Vaccine Ontology ID:
VO_0004201
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Detoxified, O-polysaccharide side chain-deficient, lipopolysaccharide non-covalently complexed with the outer membrane protein of N. meningitidis group B (Gregory et al., 2010).
e. Adjuvant: CpG DNA vaccine adjuvant
f. Immunization Route
intranasal immunization
g. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Ten, 6- to 8-week-old mice were inoculated i.n. with 1 μg J5dLPS/OMP vaccine at 0, 2, and 4 weeks. Unmethylated cytidine-phosphate-guanosine (CpG) containing oligodinucleotide (ODN 10104; Coley Pharmaceutical Group, Wellesley, MA) was administered i.n. (25 μg/mouse) one hour prior to each vaccination. Control groups received CpG ODN only (Gregory et al., 2010).
  • Challenge Protocol: Vaccinated mice were challenged i.t. with F. tularensis SchuS4 on days 35 - 42 post-vaccination (Gregory et al., 2010).
  • Efficacy: Mice vaccinated with J5dLPS/OMP and CpG ODN resisted infection by type A F. tularensis. Sixty-five percent of vaccinated animals survived challenge i.t. with 10 LD50 F. tularensis SchuS4 (Gregory et al., 2010).
22. KKF24
a. Vaccine Ontology ID:
VO_0000307
b. Type:
Live Attenuated
c. Gene Engineering of IglC
  • Type: Protein
  • Description:
  • Detailed Gene Information: Click here.
d. Preparation
KKF24 is a recombinant strain of Francisella novicida U112 with iglC mutation (F. novicida delta-iglC::ermC) (Lauriano et al., 2003). Strains were grown at 37°C in Trypticase soy broth supplemented with 0.1% cysteine (Pammit et al., 2006).
e. Virulence
KKF24 is highly attenuated for virulence in mice and growth within amoebae (Pammit et al., 2006).
f. Description
KKF24 has been identified as a live attenuated vaccine against subsequent intranasal (i.n.) challenge with the wild-type organism of Francisella tularensis (Pammit et al., 2006).
g. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were first anesthetized with 3% isoflurane using a rodent anesthesia system and then inoculated i.n. with 106 CFU of KKF24 in 25 μl of PBS. Mock-vaccinated animals were treated with PBS alone (Pammit et al., 2006).
  • Persistence: (Pammit et al., 2006)
  • Side Effects: None were noted (Pammit et al., 2006).
  • Challenge Protocol: Vaccinated mice exhibited no signs of morbidity and were challenged 30 days later i.n. with escalating inocula of the wild-type F. novicida U112 strain. Animals were monitored daily for morbidity and mortality (Pammit et al., 2006).
  • Efficacy: Intranasal vaccination with the F. novicida iglC mutant is highly efficacious against i.n. challenge with the wild-type strain (Pammit et al., 2006). Specifically, vaccinated animals challenged with 100 LD50 of U112 had an 82% survival rate, with minimal losses of body weight . When the challenge inoculum was increased to 1000 LD50 of U112, the survival rate decreased to 50%. Increasing the challenge inoculum further to 10,000 LD50 of U112 resulted in 20% survival. There was no survival of any unvaccinated animals at the challenge doses tested, indicating that all three inocula of U112 were lethal doses, as expected (Pammit et al., 2006).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: It was found that all of the IFN-γ−/− vaccinated mice quickly succumbed within 4 to 5 days to the pulmonary challenge with the wild-type strain, whereas 100% of the IFN-γ+/+ mice were completely protected against both challenge doses. Both groups were able to survive vaccination with KKF24 (Pammit et al., 2006).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighg1
    • Gene Response: Intranasal immunization with 106 CFU KKF24 induced a robust primary antibody response that included the induction of F. novicida-specific total, IgG1, and IgG2a antibodies. No binding was observed with unrelated antigens, levels in immune sera were significantly greater than normal mouse sera, and levels increased post-challenge (Pammit et al., 2006).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighv1-9
    • Gene Response: Intranasal immunization with 106 CFU KKF24 induced a robust primary antibody response that included the induction of F. novicida-specific total, IgG1, and IgG2a antibodies. No binding was observed with unrelated antigens, levels in immune sera were significantly greater than normal mouse sera, and levels increased post-challenge (Pammit et al., 2006).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Il12a
    • Gene Response: There was potent IL-12 secretion from the stimulated cells upon i.n. vaccination with KKF24 as compared to mice inoculated with PBS. Cervical lymph node and spleen cells were stimulated by UV-inactivated KKF24 10 days after vaccination and then tested for cytokines (Pammit et al., 2006).
    • Detailed Gene Information: Click here.
23. LVS
a. Vaccine Ontology ID:
VO_0000271
b. Type:
Live Attenuated
c. Preparation
F. tularensis LVS (ATCC 29684; American Type Culture Collection, Rockville, Md.) was cultured on modified Mueller-Hinton agar plates or in modified Mueller-Hinton broth, and then frozen in broth at -70C; 1-ml aliquots were periodically thawed for use. The number of CFU after thawing varied less than 5% over a 6-month period (Elkins et al., 1996).
d. Virulence
LVS is virulent for laboratory mice and causes a fulminant infection with a histopathology quite similar to that of human tularemia (Elkins et al., 1996).
e. Description
Live attenuated strain (LVS) is derived from the multiple passage of a fully virulent strain of F. tularensis subspecies holarctica and has previously been produced in the US as an investigative new drug. Numerous studies have shown the effectiveness of the LVS vaccine in humans and in animal models of the disease, and protection against aerosol challenge with F. tularensis subspecies tularensis has been demonstrated (Isherwood et al., 2005).
f. Mouse Response
  • Host Strain: nu/nu, scid
  • Vaccination Protocol: Mice were given 0.5ml i.p. or 0.1 ml i.d. of the indicated dilution of LVS. To deplete mice of circulating cytokines, mice were treated i.p. with 500 mg of anti-IFN-g, anti-TNF-a, or control hamster immunoglobulin G (IgG) 1 h before infection with LVS. To deplete mice of neutrophils, mice were treated i.p. with 250 mg of RB6-8C5 at 3 d and again at 4 h before infection with LVS for depletion of Gr-11 cells (Elkins et al., 1996).
  • Persistence: T lymphocytes must be available for clearance of bacteria and long-term survival. Populations enriched for either CD4+ or CD8+ T cells can reconstitute long-term survival and clearance of i.d. LVS infection in scid mice, but over time, reconstituted recipients contain all subpopulations (Elkins et al., 1996).
  • Efficacy: Scid mice survive i.d. infection with doses of LVS ranging from 100 to 106 for about 20 days. All surviving recipients that cleared bacteria contained both CD4+ and CD8+ T cells in their spleens, including those that originally received highly enriched CD4+ or CD8+ T cells (Elkins et al., 1996). IFN-gamma and TNF-alpha are critical cytokines for the initial survival of infection. Mice treated with anti-TNF-a antibodies or knockout mice lacking functional TNF-a receptors are extremely susceptible to Listeria infection. Similarly, GKO mice infected i.v. or by aerosol with Mycobacterium tuberculosis succumb to infection, as do IFN-gamma receptor knockout mice infected with Listeria spp. The experiments using knockout mice unequivocally demonstrate that there is no compensatory activity available during bacterial infection for IFN-gamma and TNF-a, despite the remarkable functional redundancy generally observed in the cytokine network (Elkins et al., 1996).
g. Human Response
  • Vaccination Protocol: The vaccine was administered by scarification. A single 0.6-mL drop of LVS vaccinwas placed on the volar surface of the left forearm. A bifurcated needle was used to administer 15 pricks through the drop. The skin surface was then cleaned with sterile gauze, and all excess fluid was removed. Volunteers were given no special bandage or covering for the site. There were no restrictions on showering or wetting the vaccination site. Volunteers were enrolled from 2 iterations of LVS vaccination. From the first iteration, 17 volunteers were enrolled; 10 mL of blood and inoculation site samples were obtained from each person. Baseline skin swabs from this group were performed on the volar surface of the left forearm. Post-vaccination swabs were performed directly at the site of inoculation. Four different personnel obtained skin swabs using similar techniques. In the second iteration of vaccination, swabs were obtained from 24 volunteers using the same time points, but no blood samples were obtained (Hepburn et al., 2006).
  • Persistence: LVS genomic DNA is detectable at the inoculation site up to 2 weeks after inoculation. This suggests that replicating organisms are present at the inoculation site, which poses a theoretical risk of person-to-person transmission. However, this risk of transmission is likely to be very minimal (Hepburn et al., 2006).
  • Side Effects: One volunteer recorded a temperature of 38.3°C on day 2. One volunteer developed 4×5-cm axillary lymphadenopathy 28 days after vaccination. No other volunteers had fever or lymphadenopathy. All volunteers had a take reaction. No severe erythema or deep ulcer was observed in any volunteer. During this study, there were no incidents of inadvertent inoculation of remote sites or person-to-person transmission (Hepburn et al., 2006).
  • Efficacy: LVS genomic DNA is almost universally detectable at the inoculation site for the first 2 days after LVS vaccination and is detectable in for 1–2 weeks after vaccination. Live LVS bacteria were detected in culture for the first 2 days after vaccination. While LVS DNA at the inoculation site was detected well past 2 days, this does not confirm the presence of viable bacteria past this time point (Hepburn et al., 2006).
  • Description: All but one volunteer had samples obtained at each of the 5 time points. The positive controls for the extraction of the samples taken were positive. The results of blood cultures were negative at baseline and at all time points. Additionally, PCR results of whole blood samples were negative using both tul4 and fopA assays at baseline and at all time points. Baseline skin swab samples were negative for LVS F. tularensis by real-time PCR assays. Skin swab samples were uniformly positive for LVS F. tularensis by PCR on day one, 40 of 41 samples were positive on day 2, and several samples were positive 1 and 2 weeks after vaccination. Two volunteers had negative results on day 7 or 8 but positive results on day 14 or 15. All 9 samples from day 35 were negative with both PCR assays (Hepburn et al., 2006).
h. Mouse Response
  • Immune Response: Reference is here (Rodriguez et al., 2011).
  • Host Gene Response of Il4 (interleukin 4)
    • Gene Response: Mast cells and secreted interleukin-4 (IL-4) effectively control intramacrophage replication of Francisella tularensis Live Vaccine Strain (LVS), and that mice deficient in mast cells or IL-4 receptor (IL-4R(-/-) exhibit greater susceptibility to pulmonary challenge (Rodriguez et al., 2011).
    • Detailed Gene Information: Click here.
24. rLm/iglC
a. Vaccine Ontology ID:
VO_0011347
b. Type:
Recombinant vector vaccine
c. Status:
Research
d. Antigen
Seven F. tularensis proteins including IglC (rLm/iglC) were expressed in recombinant L. monocytogenes (rLm) vaccines (Jia et al., 2009). These 7 F. tularensis proteins are: AcpA, Bfr, DnaK, GroEL, IglC, Pld and KatG from SchuS4 strain.
e. Vector:
attenuated L. monocytogenes strain rLmΔactA
f. Immunization Route
Intradermal injection (i.d.)
25. sodB(Ft)
a. Product Name:
LVS sodB mutant
b. Vaccine Ontology ID:
VO_0000263
c. Type:
Live Attenuated
d. Gene Engineering of SodB from F. tularensis SCHU S4
  • Type: Protein
  • Description:
  • Detailed Gene Information: Click here.
e. Preparation
sodBFt is made by amplifying a region of the sodB gene by PCR. The full-length sodB gene is also amplified. These amplified fragments are cloned separately into a PCR cloning vector and excised by digestion with restriction enzymes. The digested fragments are ligated simultaneously to an XbaI/SalI-digested pPV shuttle vector to yield pPV-sodB. The PstI site upstream of the start codon is used as a marker for screening of mutant colonies. pPV-sodB is then transformed in Escherichia coli S17-1 and transferred into F. tularensis LVS via conjugation. The mutants are selected on modified chocolate agar plates supplemented with Isovitalex, L-cysteine hydrochloride, 1% hemoglobin, 2 µg/ml chloramphenicol, and 100 µg/ml polymyxin B. Colonies obtained on the chloramphenicol plates following the first recombination event are analyzed by PCR, followed by the digestion of PCR products with PstI enzyme. The positive mutant colonies are selected for a second recombination event by plating on medium containing 5% sucrose. Chloramphenicol sensitivity and sucrose resistance following the second recombination event are confirmed by PCR and PstI digestion of the amplified products. The positive mutant clone is referred to as sodBFt (Bakshi et al., 2006).
f. Virulence
sodB(Ft) has increased sensitivity to paraquat and hydrogen peroxide (H2O2), the redox cycling compounds, as well as with reduced virulence in both C57BL/6 and BALB/c mice (Bakshi et al., 2006).
g. Description
sodBFt is a live vaccine strain mutant of Francisella tularensis with reduced Fe-superoxide dismutase gene expression (Bakshi et al., 2006).
h. Mouse Response
  • Host Strain: C57BL/6, BALB/c
  • Vaccination Protocol: Mice were immunized i.n. with 5 × 103 CFU of LVS or sodBFt in a volume of 20 μl PBS (10 μl/nare). Unvaccinated mice, which served as a control, received an equal volume of PBS (Bakshi et al., 2008).
  • Persistence: (Bakshi et al., 2006)
  • Side Effects: None were noted (Bakshi et al., 2006).
  • Challenge Protocol: Mice immunized with 5 × 103 CFU of either LVS or sodBFt were challenged i.n. with 1 × 101 CFU (10LD100) of SchuS4 on day 21 post-immunization (Bakshi et al., 2008).
  • Efficacy: sodBFt vaccinated C57BL/6 mice not only had a significantly extended MTD (median time to death) as compared to LVS vaccinated or unvaccinated mice, but, 40% (4/10) of the sodBFt vaccinated mice survived the challenge. All naïve C57BL/6 mice challenged with a similar dose of SchuS4 strain succumbed to infection within 6–8 days post-challenge (Bakshi et al., 2008).
V. References
1. Bakshi et al., 2006: Bakshi CS, Malik M, Regan K, Melendez JA, Metzger DW, Pavlov VM, Sellati TJ. Superoxide dismutase B gene (sodB)-deficient mutants of Francisella tularensis demonstrate hypersensitivity to oxidative stress and attenuated virulence. Journal of bacteriology. 2006 Sep; 188(17); 6443-8. [PubMed: 16923916].
2. Bakshi et al., 2008: Bakshi CS, Malik M, Mahawar M, Kirimanjeswara GS, Hazlett KR, Palmer LE, Furie MB, Singh R, Melendez JA, Sellati TJ, Metzger DW. An improved vaccine for prevention of respiratory tularemia caused by Francisella tularensis SchuS4 strain. Vaccine. 2008; 26(41); 5276-5288. [PubMed: 18692537].
3. Bokhari et al., 2008: Bokhari SM, Kim KJ, Pinson DM, Slusser J, Yeh HW, Parmely MJ. NK cells and gamma interferon coordinate the formation and function of hepatic granulomas in mice infected with the Francisella tularensis live vaccine strain. Infection and immunity. 2008; 76(4); 1379-1389. [PubMed: 18227174].
4. Breitbach et al., 2008: Breitbach K, Köhler J, Steinmetz I. Induction of protective immunity against Burkholderia pseudomallei using attenuated mutants with defects in the intracellular life cycle. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2008; 102 Suppl 1; S89-94. [PubMed: 19121696].
5. Cong et al., 2009: Cong Y, Yu JJ, Guentzel MN, Berton MT, Seshu J, Klose KE, Arulanandam BP. Vaccination with a defined Francisella tularensis subsp. novicida pathogenicity island mutant (DeltaiglB) induces protective immunity against homotypic and heterotypic challenge. Vaccine. 2009; 27(41); 5554-5561. [PubMed: 19651173].
6. Conlan et al., 2010: Conlan JW, Shen H, Golovliov I, Zingmark C, Oyston PC, Chen W, House RV, Sjöstedt A. Differential ability of novel attenuated targeted deletion mutants of Francisella tularensis subspecies tularensis strain SCHU S4 to protect mice against aerosol challenge with virulent bacteria: effects of host background and route of immunization. Vaccine. 2010; 28(7); 1824-1831. [PubMed: 20018266].
7. Dreisbach et al., 2000: Dreisbach VC, Cowley S, Elkins KL. Purified lipopolysaccharide from Francisella tularensis live vaccine strain (LVS) induces protective immunity against LVS infection that requires B cells and gamma interferon. Infection and immunity. 2000 Apr; 68(4); 1988-96. [PubMed: 10722593].
8. Elkins et al., 1996: Elkins KL, Rhinehart-Jones TR, Culkin SJ, Yee D, Winegar RK. Minimal requirements for murine resistance to infection with Francisella tularensis LVS. Infection and immunity. 1996 Aug; 64(8); 3288-93. [PubMed: 8757866].
9. Gregory et al., 2010: Gregory SH, Chen WH, Mott S, Palardy JE, Parejo NA, Heninger S, Anderson CA, Artenstein AW, Opal SM, Cross AS. Detoxified endotoxin vaccine (J5dLPS/OMP) protects mice against lethal respiratory challenge with Francisella tularensis SchuS4. Vaccine. 2010; 28(16); 2908-2915. [PubMed: 20170768].
10. Hartley et al., 2004: Hartley MG, Green M, Choules G, Rogers D, Rees DG, Newstead S, Sjostedt A, Titball RW. Protection afforded by heat shock protein 60 from Francisella tularensis is due to copurified lipopolysaccharide. Infection and immunity. 2004; 72(7); 4109-4113. [PubMed: 15213156].
11. Hepburn et al., 2006: Hepburn MJ, Purcell BK, Lawler JV, Coyne SR, Petitt PL, Sellers KD, Norwood DA, Ulrich MP. Live vaccine strain Francisella tularensis is detectable at the inoculation site but not in blood after vaccination against tularemia. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2006 Sep 15; 43(6); 711-6. [PubMed: 16912944].
12. Isherwood et al., 2005: Isherwood KE, Titball RW, Davies DH, Felgner PL, Morrow WJ. Vaccination strategies for Francisella tularensis. Advanced drug delivery reviews. 2005 Jun 17; 57(9); 1403-14. [PubMed: 15919131].
13. Jayakar et al., 2011: Jayakar HR, Parvathareddy J, Fitzpatrick EA, Bina XR, Bina JE, Re F, Emery FD, Miller MA. A galU Mutant of Francisella tularensis is Attenuated for Virulence in a Murine Pulmonary Model of Tularemia. BMC microbiology. 2011; 11(1); 179. [PubMed: 21819572].
14. Jia et al., 2009: Jia Q, Lee BY, Clemens DL, Bowen RA, Horwitz MA. Recombinant attenuated Listeria monocytogenes vaccine expressing Francisella tularensis IglC induces protection in mice against aerosolized Type A F. tularensis. Vaccine. 2009; 27(8); 1216-1229. [PubMed: 19126421].
15. Jia et al., 2010: Jia Q, Lee BY, Bowen R, Dillon BJ, Som SM, Horwitz MA. A Francisella tularensis live vaccine strain (LVS) mutant with a deletion in capB, encoding a putative capsular biosynthesis protein, is significantly more attenuated than LVS yet induces potent protective immunity in mice against F. tularensis challenge. Infection and immunity. 2010; 78(10); 4341-4355. [PubMed: 20643859].
16. Lauriano et al., 2003: Lauriano CM, Barker JR, Nano FE, Arulanandam BP, Klose KE. Allelic exchange in Francisella tularensis using PCR products. FEMS microbiology letters. 2003 Dec 12; 229(2); 195-202. [PubMed: 14680699].
17. Li et al., 2007: Li J, Ryder C, Mandal M, Ahmed F, Azadi P, Snyder DS, Pechous RD, Zahrt T, Inzana TJ. Attenuation and protective efficacy of an O-antigen-deficient mutant of Francisella tularensis LVS. Microbiology (Reading, England). 2007; 153(Pt 9); 3141-3153. [PubMed: 17768257].
18. Melillo et al., 2009: Melillo AA, Mahawar M, Sellati TJ, Malik M, Metzger DW, Melendez JA, Bakshi CS. Identification of Francisella tularensis live vaccine strain CuZn superoxide dismutase as critical for resistance to extracellularly generated reactive oxygen species. Journal of bacteriology. 2009; 191(20); 6447-6456. [PubMed: 19684141].
19. Pammit et al., 2006: Pammit MA, Raulie EK, Lauriano CM, Klose KE, Arulanandam BP. Intranasal vaccination with a defined attenuated Francisella novicida strain induces gamma interferon-dependent antibody-mediated protection against tularemia. Infection and immunity. 2006 Apr; 74(4); 2063-71. [PubMed: 16552035].
20. Pechous et al., 2006: Pechous R, Celli J, Penoske R, Hayes SF, Frank DW, Zahrt TC. Construction and characterization of an attenuated purine auxotroph in a Francisella tularensis live vaccine strain. Infection and immunity. 2006; 74(8); 4452-4461. [PubMed: 16861631].
21. Quarry et al., 2007: Quarry JE, Isherwood KE, Michell SL, Diaper H, Titball RW, Oyston PC. A Francisella tularensis subspecies novicida purF mutant, but not a purA mutant, induces protective immunity to tularemia in mice. Vaccine. 2007; 25(11); 2011-2018. [PubMed: 17241711].
22. Rodriguez et al., 2011: Rodriguez AR, Yu JJ, Murthy AK, Guentzel MN, Klose KE, Forsthuber TG, Chambers JP, Berton MT, Arulanandam BP. Mast cell/IL-4 control of Francisella tularensis replication and host cell death is associated with increased ATP production and phagosomal acidification. Mucosal immunology. 2011; 4(2); 217-226. [PubMed: 20861832].
23. Rohmer et al., 2006: Rohmer L, Brittnacher M, Svensson K, Buckley D, Haugen E, Zhou Y, Chang J, Levy R, Hayden H, Forsman M, Olson M, Johansson A, Kaul R, Miller SI. Potential source of Francisella tularensis live vaccine strain attenuation determined by genome comparison. Infection and immunity. 2006 Sep 25; ; . [PubMed: 17000723].
24. Sammons-Jackson et al., 2008: Sammons-Jackson WL, McClelland K, Manch-Citron JN, Metzger DW, Bakshi CS, Garcia E, Rasley A, Anderson BE. Generation and characterization of an attenuated mutant in a response regulator gene of Francisella tularensis live vaccine strain (LVS). DNA and cell biology. 2008; 27(7); 387-403. [PubMed: 18613792].
25. Santiago et al., 2009: Santiago AE, Cole LE, Franco A, Vogel SN, Levine MM, Barry EM. Characterization of rationally attenuated Francisella tularensis vaccine strains that harbor deletions in the guaA and guaB genes. Vaccine. 2009; 27(18); 2426-2436. [PubMed: 19368784].
26. Savitt et al., 2009: Savitt AG, Mena-Taboada P, Monsalve G, Benach JL. Francisella tularensis infection-derived monoclonal antibodies provide detection, protection, and therapy. Clinical and vaccine immunology : CVI. 2009; 16(3); 414-422. [PubMed: 19176692].
27. Sebastian et al., 2007: Sebastian S, Dillon ST, Lynch JG, Blalock LT, Balon E, Lee KT, Comstock LE, Conlan JW, Rubin EJ, Tzianabos AO, Kasper DL. A defined O-antigen polysaccharide mutant of Francisella tularensis live vaccine strain has attenuated virulence while retaining its protective capacity. Infection and immunity. 2007; 75(5); 2591-2602. [PubMed: 17296751].
28. Sjöstedt et al., 1992: Sjöstedt A, Sandström G, Tärnvik A. Humoral and cell-mediated immunity in mice to a 17-kilodalton lipoprotein of Francisella tularensis expressed by Salmonella typhimurium. Infection and immunity. 1992; 60(7); 2855-2862. [PubMed: 1612751].
29. Tempel et al., 2006: Tempel R, Lai XH, Crosa L, Kozlowicz B, Heffron F. Attenuated Francisella novicida transposon mutants protect mice against wild-type challenge. Infection and immunity. 2006 Sep; 74(9); 5095-105. [PubMed: 16926401].
30. Twine et al., 2005: Twine S, Bystrom M, Chen W, Forsman M, Golovliov I, Johansson A, Kelly J, Lindgren H, Svensson K, Zingmark C, Conlan W, Sjostedt A. A mutant of Francisella tularensis strain SCHU S4 lacking the ability to express a 58-kilodalton protein is attenuated for virulence and is an effective live vaccine. Infection and immunity. 2005 Dec; 73(12); 8345-52. [PubMed: 16299332 ].
31. Yu et al., 2010: Yu JJ, Goluguri T, Guentzel MN, Chambers JP, Murthy AK, Klose KE, Forsthuber TG, Arulanandam BP. Francisella tularensis T-cell antigen identification using humanized HLA-DR4 transgenic mice. Clinical and vaccine immunology : CVI. 2010; 17(2); 215-222. [PubMed: 20016043].