VIOLIN: Vaccine Investigation and Online Information Network

Ebola virus

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. GP from Cote d'Ivoire Ebola virus (Other)
2. NP from Zaire Ebola virus (Other)
3. EBOV NP (Protective antigen)
4. GP from Reston ebolavirus (Protective antigen)
5. GP from Sudan ebolavirus (Protective antigen)
6. GP from Zaire ebolavirus (Protective antigen)
7. NP (Protective antigen)
8. pagA (Protective antigen)
9. SGP (Protective antigen)
10. VP24 (Protective antigen)
11. VP24 from Reston ebolavirus (Protective antigen)
12. VP24 from Zaire ebolavirus (Protective antigen, Protective antigen)
13. VP30 (Protective antigen)
14. VP30 from Reston ebolavirus (Protective antigen)
15. VP30 from Zaire ebolavirus (Protective antigen, Protective antigen)
16. VP35 (Protective antigen)
17. VP35 from Reston ebolavirus (Protective antigen)
18. VP35 from Zaire ebolavirus (Protective antigen, Protective antigen)
19. VP40 from Reston ebolavirus (Protective antigen)
20. VP40 from Zaire ebolavirus (Protective antigen)
21. ZGP (Protective antigen)
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

205488

2. Disease:

Ebola hemorrhagic fever

3. Introduction

Ebola virus is an aggressive pathogen that causes a highly lethal hemorrhagic fever syndrome in humans and nonhuman primates. Typically, Ebola virus infection runs its course within 14 to 21 days. Infection initially presents with nonspecific flu-like symptoms such as fever, myalgia, and malaise. As the infection progresses, patients exhibit severe bleeding and coagulation abnormalities, including gastrointestinal bleeding, rash, and a range of hematological irregularities, such as lymphopenia and neutrophilia. Cytokines are released when reticuloendothelial cells encounter virus, which can contribute to exaggerated inflammatory responses that are not protective. Damage to the liver, combined with massive viremia, leads to disseminated intravascular coagulopathy. The virus eventually infects microvascular endothelial cells and compromises vascular integrity. The terminal stages of Ebola virus infection usually include diffuse bleeding, and hypotensive shock accounts for many Ebola virus fatalities (Sullivan et al., 2003).

The Ebola virus genome is 19 kb long, with seven open reading frames encoding structural proteins, including the virion envelope glycoprotein (GP), nucleoprotein (NP), and matrix proteins VP24 and VP40; nonstructural proteins, including VP30 and VP35; and the viral polymerase. The GP open reading frame of Ebola virus gives rise to two gene products, a soluble 60- to 70-kDa protein (sGP) and a full-length 150- to 170-kDa protein (GP) that inserts into the viral membrane through transcriptional editing (Sullivan et al., 2003).

4. Microbial Pathogenesis

Mononuclear phagocytes are the first targets of infection relevant to disease pathogenesis, followed by connective tissues and parenchymal cells. Cytokines are released when reticuloendothelial cells encounter virus, which can contribute to exaggerated nonprotective inflammatory responses. Ebola virus GP plays a vital role in infection. Virual GP appears to form a trimeric complex and binds preferentially to endothelial cells Infection of endothelial cells also induces a cytopathic effect and damage to the endothelial barrier that, together with cytokine effects, leads to the loss of vascular integrity. Cytokine dysregulation and virus infection may synergize at the endothelial surface, promoting hemorrhage and vasomotor collapse. Severity of infection is influenced by age, immune status, and viral virulence. Infection with species-adapted viruses may be lethal. The amount and location of fibin deposits varies with animal species. Strains show differing levels of virulence both across species and by route of administration. Microvascular damage and activation of the clotting cascade occurs. Death is secondary to massive cell death, fluid shifts, hemorrhages, and vascular abnormalities (Sullivan et al., 2003a).

Link to pathogenesis of Ebola virus in HazARD.

5. Host Ranges and Animal Models

The natural host for Ebola virus is unknown (Sullivan et al., 2003).

6. Host Protective Immunity

Both adaptive and innate inflammatory systems respond to infection (Sullivan et al., 2003a). Although antibody titres correlate with the protective response, many studies in non-human primates have suggested that the passive transfer of antibody is insufficient to provide long-lasting protection against Ebola virus (Sullivan et al., 2003b). In rodent studies with adapted Ebola virus, passive transfer of antibodies or adoptive transfer of cytotoxic T cells showed protection when given before infection. A more sensitive but less quantitative CD4 lympho-proliferative response correlated with protection in a DNA/ADV prime–boost study. In addition to the antibody response induced by an effective vaccine, both CD4 and CD8 responses were observed after the challenge. The fact that CD4 responses were not observed before challenge whereas CD8 responses were more consistently seen beforehand suggests that the CD8 response is likely to have an important role in protection in non-human primates (Sullivan et al., 2003b).

II. Vaccine Related Pathogen Genes

1. EBOV NP

Gene Name : EBOV NP
Sequence Strain (Species/Organism) : Reston ebolavirus
VO ID : VO_0010859
NCBI Gene ID : 955194
NCBI Protein GI : 22789223
Locus Tag : REBOVgp1
Genbank Accession : AF522874
Protein Accession : NP_690580
Taxonomy ID : 186539
Gene Starting Position : 55
Gene Ending Position : 3012
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 4.69
Protein Weight : 80076.91
Protein Length : 739

DNA Sequence : Show Sequence

>NC_004161.1:55-3012 Reston ebolavirus isolate Reston virus/M.fascicularis-tc/USA/1989/Philippines89-Pennsylvania, complete genome
TGAGGAAGATTAACAGTTTTCCTCAGTTTAAGATATACACTGAAATTGAGATTGAGATTCTCCTCTTTGC
TATTCTGTAACTTTCCCTGGTTGTGACAATTGAATCAGTTTTATCTATTACCAATTACCATCAACATGGT
ATGTCTAGTGATCTTGGGACTCTTCTTCATCTGGTTTTTCCTAGAGCTCTGAATCCATTTTGCGAGAAGT
TCATCCAAACGACCCAGTGTCTGAAAATACAAAAGGTTCCCCTTTCCGTCAAGTTTAAGGGGTTGTTTTG
ATTGTGTGTAGATTTTATAATCCTAGAGTGCCAAGGAGTTGCGTGTCATCATTGATTGGGAAGATCAAGG
AAACAATTTGTTCCAATAATATCGTACATCTTGACTAAGTCGAACAAGGGGAAGTCGATATGGATCGTGG
GACCAGAAGAATCTGGGTGTCGCAAAATCAAGGTGATACTGATTTAGATTATCATAAAATTTTGACAGCT
GGCCTTACTGTTCAACAGGGAATTGTCAGGCAGAAAATAATTTCTGTATATCTTGTTGATAACTTGGAGG
CTATGTGTCAATTGGTAATACAAGCCTTTGAGGCCGGAATTGATTTCCAAGAAAATGCCGACAGCTTCCT
TCTGATGCTTTGCCTACATCATGCTTACCAAGGTGACTATAAATTGTTCTTGGAGAGCAATGCTGTACAG
TATTTGGAAGGTCATGGATTCAAATTTGAGCTCCGGAAGAAGGACGGTGTCAATCGGCTCGAGGAATTGC
TTCCTGCTGCAACGAGTGGAAAAAACATCAGGCGTACGTTGGCCGCACTGCCTGAAGAGGAGACTACAGA
AGCAAATGCAGGGCAATTTCTCTCATTTGCGAGTTTGTTTCTTCCCAAACTGGTTGTGGGAGAGAAGGCT
TGCTTGGAAAAAGTCCAGCGACAAATTCAGGTTCATGCAGAACAGGGTTTAATTCAATATCCCACTGCAT
GGCAATCAGTTGGACACATGATGGTAATCTTCAGATTGATGAGGACTAATTTCTTGATTAAATATTTACT
GATCCACCAGGGTATGCATATGGTAGCTGGCCACGATGCCAATGATGCTGTCATTGCTAATTCAGTTGCT
CAGGCTCGCTTTTCAGGACTCCTAATTGTCAAAACCGTTCTTGATCATATTCTGCAAAAAACCGACCAAG
GAGTAAGACTTCACCCTTTGGCCCGAACAGCCAAAGTGCGTAATGAGGTTAATGCATTTAAGGCCGCCCT
AAGCTCACTTGCTAAGCATGGGGAATATGCCCCTTTTGCTCGCCTTCTCAATCTCTCGGGAGTTAACAAC
CTAGAACATGGTCTCTACCCACAGTTATCAGCAATTGCTCTTGGAGTTGCCACAGCACATGGTAGCACCC
TTGCAGGAGTTAATGTTGGTGAGCAGTATCAGCAGCTTAGAGAGGCTGCCACTGAAGCTGAGAAGCAACT
CCAACAATATGCTGAGTCCAGAGAACTCGACAGCCTAGGCCTGGACGATCAGGAAAGAAGAATACTAATG
AACTTCCATCAGAAGAAAAACGAAATTAGTTTCCAGCAGACCAATGCAATGGTAACCCTTAGGAAAGAGC
GACTGGCTAAATTAACAGAAGCTATAACGCTGGCCTCAAGACCTAACCTCGGGTCTAGACAAGACGACGG
CAATGAAATACCGTTCCCTGGGCCTATAAGCAACAACCCAGACCAAGATCATCTGGAGGATGATCCTAGA
GACTCCAGAGACACCATCATTCCTAATGGTGCAATTGACCCCGAGGATGGTGATTTTGAAAATTACAATG
GCTATCATGATGATGAAGTTGGGACGGCAGGTGACTTGGTCCTGTTCGATCTTGACGATCATGAGGATGA
CAATAAAGCTTTTGAGCCACAGGACAGCTCGCCACAATCCCAAAGGGAAATAGAGAGAGAAAGATTAATT
CATCCACCCCCAGGCAACAACAAGGACGACAATCGAGCCTCAGACAACAATCAACAATCAGCAGATTCTG
AGGAACAAGGAGGTCAATACAACTGGCACCGAGGCCCAGAACGTACGACCGCCAATCGAAGACTCTCACC
AGTGCACGAAGAGGACACCCTTATGGATCAAGGTGATGATGATCCCTCAAGCTTACCTCCGCTGGAATCT
GATGATGACGATGCATCAAGTAGCCAACAAGATCCCGATTATACAGCTGTTGCCCCTCCTGCTCCTGTAT
ACCGCAGTGCAGAAGCCCACGAGCCTCCCCACAAATCCTCGAACGAGCCAGCTGAAACATCACAATTGAA
TGAAGACCCTGATATCGGTCAATCAAAGTCTATGCAAAAATTAGAAGAGACATATCACCATCTGCTGAGA
ACTCAAGGTCCATTTGAAGCCATCAATTATTATCACATGATGAAGGATGAGCCGGTAATATTTAGCACTG
ATGATGGGAAGGAATACACCTACCCGGATTCACTTGAGGAAGCCTATCCTCCATGGCTCACCGAGAAAGA
ACGACTGGACAAAGAGAATCGCTACATTTACATAAATAATCAACAGTTCTTCTGGCCTGTCATGAGTCCC
AGAGACAAATTTCTTGCAATCTTGCAGCACCATCAGTAACCACAGCACAAAGCGCGGTCCACTTCGTAAA
GCTAAATACACTTAAGACTTGACCGATTCATCTACAAAAACTAATCCATTATAACTTATTAGTGCTACTT
TTCTATAAGTGATTCTTAATCTAAGGCCATTAAGAGTTTAAGTAATATACATATACACTTACACCGGTCT
ATCCAAGATGTGGCTCAATGTTCTTGATTTGAACATAGTCATAAGGGGATAAATAATACTTTATATTTCT
GATTGTGGATTGACCCATTCTGCTTAAAATGCTTCGCCCATTGAAAATGTGATCTAATAGATAGCCCTGA
CTAGACAAATTAAGAAAA

Protein Sequence : Show Sequence

>NP_690580.1 nucleoprotein [Reston ebolavirus]
MDRGTRRIWVSQNQGDTDLDYHKILTAGLTVQQGIVRQKIISVYLVDNLEAMCQLVIQAFEAGIDFQENA
DSFLLMLCLHHAYQGDYKLFLESNAVQYLEGHGFKFELRKKDGVNRLEELLPAATSGKNIRRTLAALPEE
ETTEANAGQFLSFASLFLPKLVVGEKACLEKVQRQIQVHAEQGLIQYPTAWQSVGHMMVIFRLMRTNFLI
KYLLIHQGMHMVAGHDANDAVIANSVAQARFSGLLIVKTVLDHILQKTDQGVRLHPLARTAKVRNEVNAF
KAALSSLAKHGEYAPFARLLNLSGVNNLEHGLYPQLSAIALGVATAHGSTLAGVNVGEQYQQLREAATEA
EKQLQQYAESRELDSLGLDDQERRILMNFHQKKNEISFQQTNAMVTLRKERLAKLTEAITLASRPNLGSR
QDDGNEIPFPGPISNNPDQDHLEDDPRDSRDTIIPNGAIDPEDGDFENYNGYHDDEVGTAGDLVLFDLDD
HEDDNKAFEPQDSSPQSQREIERERLIHPPPGNNKDDNRASDNNQQSADSEEQGGQYNWHRGPERTTANR
RLSPVHEEDTLMDQGDDDPSSLPPLESDDDDASSSQQDPDYTAVAPPAPVYRSAEAHEPPHKSSNEPAET
SQLNEDPDIGQSKSMQKLEETYHHLLRTQGPFEAINYYHMMKDEPVIFSTDDGKEYTYPDSLEEAYPPWL
TEKERLDKENRYIYINNQQFFWPVMSPRDKFLAILQHHQ

Molecule Role : Protective antigen
Related Vaccine(s): GP-VRP , NP-VRP

2. GP from Cote d'Ivoire Ebola virus

Gene Name : GP from Cote d'Ivoire Ebola virus
Sequence Strain (Species/Organism) : Cote d'Ivoire ebolavirus
NCBI Gene ID : 9487535
NCBI Protein GI : 302315373
Locus Tag : CIEBOVp4
Genbank Accession : FJ217162
Protein Accession : YP_003815426
Taxonomy ID : 186541
Gene Starting Position : 5887
Gene Ending Position : 8292
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 6.6
Protein Weight : 70705.96
Protein Length : 676
Protein Note : small non-structural secreted glycoprotein; sGP

DNA Sequence : Show Sequence

>gi|302315369:5887-8292 Cote d'Ivoire ebolavirus
TGATGAAGATTAAGGCAACCAGTGGTGCTATCTTCATCTCTTTGATTTGAGTCTTAAGTGAATACACAGG
TTCTAATACTGTTCTTCTGTCCAACGGTATAATTCAGCCAGGCCTAAGACAGTAGCTAATCACAGTCATC
ATGGGAGCGTCAGGGATTCTGCAATTGCCCCGTGAGCGCTTCAGGAAAACATCTTTCTTTGTTTGGGTAA
TAATCCTATTCCATAAAGTCTTTTCAATCCCGTTGGGGGTTGTACACAACAATACCCTACAAGTGAGTGA
TATTGACAAGTTTGTGTGCCGAGACAAACTCTCTTCAACTAGCCAATTGAAGTCAGTCGGGTTGAACTTG
GAGGGCAATGGAGTAGCAACTGATGTACCAACGGCAACCAAAAGATGGGGTTTTCGAGCTGGTGTTCCAC
CAAAGGTGGTAAATTGCGAAGCTGGAGAATGGGCTGAGAACTGTTATAACCTGGCTATAAAGAAAGTTGA
TGGTAGTGAGTGCCTACCAGAAGCCCCTGAGGGAGTGAGGGATTTTCCCCGTTGCCGCTATGTACACAAA
GTCTCAGGAACTGGACCATGCCCAGGAGGACTCGCCTTTCACAAAGAAGGAGCCTTCTTCCTGTATGACC
GACTCGCATCAACAATCATTTATCGGGGTACAACCTTTGCCGAAGGAGTTATTGCATTTCTGATCTTGCC
TAAGGCGCGAAAGGATTTTTTCCAGTCTCCTCCATTGCATGAGCCTGCCAACATGACCACGGATCCCTCC
AGTTACTATCACACGACAACAATAAACTACGTGGTTGATAATTTTGGAACCAACACCACAGAGTTTCTGT
TCCAAGTCGATCATTTGACGTATGTGCAGCTCGAGGCAAGATTCACACCACAATTCCTTGTCCTCCTAAA
TGAAACCATCTACTCTGATAACCGCAGAAGTAACACAACAGGAAAACTAATCTGGAAAATAAATCCCACT
GTTGATACCAGCATGGGTGAGTGGGCTTTCTGGGAAAATAAAAAAACTTCACAAAAACCCTTTCAAGTGA
AGAGTTGTCTTTCGTACCTGTACCAGAAACCCAGAACCAGGTCCTTGACACGACAGCGACGGTCTCTCCT
CCCATCTCCGCCCACAACCACGCAGCCGAAGACCACAAAGAATTGGTTTCAGAGGATTCCACTCCAGTGG
TTCAGATGCAAAACATCAAGGGAAAGGACACAATGCCAACCACAGTGACGGGTGTACCAACAACCACACC
CTCTCCATTTCCAATCAATGCTCGCAACACTGATCATACCAAATCATTTATCGGCCTGGAGGGGCCCCAA
GAAGACCACAGCACCACACAGCCTGCCAAGACCACCAGCCAACCAACCAACAGCACAGAATCGACGACAC
TAAACCCAACATCAGAGCCCTCCAGTAGAGGCACGGGACCATCCAGCCCCACGGTCCCCAACACCACAGA
AAGCCACGCCGAACTTGGCAAGACAACCCCAACCACACTCCCAGAACAGCACACTGCCGCCAGTGCCATT
CCAAGAGCCGTGCACCCCGACGAACTCAGTGGACCTGGCTTCCTGACGAACACAATACGGGGGGTTACAA
ATCTCCTGACAGGATCCAGAAGAAAGCGAAGGGATGTCACTCCCAATACACAACCCAAATGCAACCCAAA
CCTGCACTATTGGACAGCCTTGGATGAGGGTGCTGCCATAGGTTTAGCCTGGATACCATACTTCGGGCCA
GCAGCTGAGGGAATTTACACTGAAGGCATAATGGAGAATCAAAATGGATTGATCTGTGGATTGAGGCAGC
TGGCCAACGAAACGACACAAGCTCTTCAATTGTTCTTAAGGGCAACTACTGAGTTGCGTACATTCTCTAT
ACTAAATCGGAAAGCAATAGACTTCTTGCTCCAAAGATGGGGAGGAACATGTCACATTCTAGGGCCTGAT
TGTTGCATTGAACCCCAAGATTGGACCAAAAATATCACTGATAAAATTGATCAAATAATCCATGACTTTG
TCGATAATAATCTTCCAAATCAGAATGATGGCAGCAACTGGTGGACTGGATGGAAACAATGGGTTCCTGC
TGGAATAGGAATCACAGGAGTAATCATTGCTATTATTGCTTTGCTGTGCATTTGCAAATTCATGCTTTGA
ACTAATATAGCATCATACTTTCTAATATTCCCCCAATATGAATTTTTGTTTTCGATTTTATTTAATGATA
TATCCTCTGTATACCTCACTAATGTACTCGAGCATAATTTCCCTGATAGACTTGATTGTATTTGATGATT
AAGGACCTCACAAAATTCCTGGGGATTGAAAAGAACTGGATAACTCAATAAATTTTATGCTAGGACCACA
AATACACTTGATGAAGATTAAGAAAA

Protein Sequence : Show Sequence

>gi|302315373|ref|YP_003815426.1| spike glycoprotein precursor [Tai Forest ebolavirus]
MGASGILQLPRERFRKTSFFVWVIILFHKVFSIPLGVVHNNTLQVSDIDKFVCRDKLSSTSQLKSVGLNL
EGNGVATDVPTATKRWGFRAGVPPKVVNCEAGEWAENCYNLAIKKVDGSECLPEAPEGVRDFPRCRYVHK
VSGTGPCPGGLAFHKEGAFFLYDRLASTIIYRGTTFAEGVIAFLILPKARKDFFQSPPLHEPANMTTDPS
SYYHTTTINYVVDNFGTNTTEFLFQVDHLTYVQLEARFTPQFLVLLNETIYSDNRRSNTTGKLIWKINPT
VDTSMGEWAFWENKKNFTKTLSSEELSFVPVPETQNQVLDTTATVSPPISAHNHAAEDHKELVSEDSTPV
VQMQNIKGKDTMPTTVTGVPTTTPSPFPINARNTDHTKSFIGLEGPQEDHSTTQPAKTTSQPTNSTESTT
LNPTSEPSSRGTGPSSPTVPNTTESHAELGKTTPTTLPEQHTAASAIPRAVHPDELSGPGFLTNTIRGVT
NLLTGSRRKRRDVTPNTQPKCNPNLHYWTALDEGAAIGLAWIPYFGPAAEGIYTEGIMENQNGLICGLRQ
LANETTQALQLFLRATTELRTFSILNRKAIDFLLQRWGGTCHILGPDCCIEPQDWTKNITDKIDQIIHDF
VDNNLPNQNDGSNWWTGWKQWVPAGIGITGVIIAIIALLCICKFML

Molecule Role : Other
Related Vaccine(s): rVSV-EBOV

3. GP from Reston ebolavirus

Gene Name : GP from Reston ebolavirus
Sequence Strain (Species/Organism) : Reston ebolavirus
VO ID : VO_0010858
NCBI Gene ID : 955190
NCBI Protein GI : 22789230
Locus Tag : REBOVgp4
Genbank Accession : AF522874
Protein Accession : NP_690583
Taxonomy ID : 186539
Gene Starting Position : 5900
Gene Ending Position : 8255
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 6.35
Protein Weight : 71040.95
Protein Length : 677

DNA Sequence : Show Sequence

>NC_004161.1:5900-8255 Reston ebolavirus isolate Reston virus/M.fascicularis-tc/USA/1989/Philippines89-Pennsylvania, complete genome
CGATGAAGATTAAGGCGACAACGAGCCGAAACTTCATCTCTTTTAAAGATCTAACATTATCTGTTCCAAA
GTCATACAAGGACACATTCAAATCAGGGATTGTAAGCTGCTATTTCTTACCTCCCCAAATTACCTATACA
ACATGGGGTCAGGATATCAACTTCTCCAATTGCCTCGGGAACGTTTTCGTAAAACTTCGTTCTTAGTATG
GGTAATCATCCTCTTCCAGCGAGCAATCTCCATGCCGCTTGGTATAGTGACAAATAGCACTCTCAAAGCA
ACAGAAATTGATCAATTGGTTTGTCGGGACAAACTGTCATCAACCAGTCAGCTCAAGTCTGTGGGGCTGA
ATCTGGAAGGAAATGGAATTGCAACCGATGTCCCATCAGCAACAAAACGCTGGGGATTTCGTTCAGGTGT
GCCTCCCAAGGTGGTCAGCTATGAAGCCGGAGAATGGGCAGAAAATTGCTACAATCTGGAGATCAAAAAG
TCAGACGGAAGTGAATGCCTCCCTCTCCCTCCCGACGGTGTACGAGGATTCCCTAGATGTCGCTATGTCC
ACAAAGTTCAAGGAACAGGTCCTTGTCCTGGTGACTTAGCTTTCCATAAAAATGGGGCTTTTTTCTTGTA
TGATAGATTGGCCTCAACTGTCATCTACCGAGGGACAACTTTTGCTGAAGGTGTCGTAGCTTTTTTAATT
CTGTCAGAGCCCAAGAAGCATTTTTGGAAGGCTACACCAGCTCATGAACCGGTGAACACAACAGATGATT
CCACAAGCTACTACATGACCCTGACACTCAGCTACGAGATGTCAAATTTTGGGGGCAATGAAAGCAACAC
CCTTTTTAAGGTAGACAACCACACATATGTGCAACTAGATCGTCCACACACTCCGCAGTTCCTTGTTCAG
CTCAATGAAACACTTCGAAGAAATAATCGCCTTAGCAACAGTACAGGGAGATTGACTTGGACATTGGATC
CTAAAATTGAACCAGATGTTGGTGAGTGGGCCTTCTGGGAAACTAAAAAAACTTTTCCCAACAACTTCAT
GGAGAAAACTTGCATTTCCAAATTCCATCAACCCACACCAACAACTCCTCAGATCAGAGCCCGGCGGGAA
CTGTCCAAGGAAAAATTAGCTACCACCCACCCGCCAACAACTCCGAGCTGGTTCCAACGGATTCCCCTCC
AGTGGTTTCAGTGCTCACTGCAGGACGGACAGAGGAAATGTCGACCCAAGGTCTAACCAACGGAGAGACA
ATCACAGGTTTCACCGCGAACCCAATGACAACCACCATTGCCCCAAGTCCAACCATGACAAGCGAGGTTG
ATAACAATGTACCAAGTGAACAGCCGAACAACACAGCATCCATTGAAGACTCCCCCCCATCGGCAAGCAA
CGAGACAATTTACCACTCCGAGATGGATCCGATCCAAGGCTCGAACAACTCCGCCCAGAGCCCACAGACC
AAGACCACGCCAGCACCCACAACATCCCCGATGACCCAGGACCCGCAAGAGACGGCCAACAGCAGCAAAC
CAGGAACCAGCCCAGGAAGCGCAGCCGGACCAAGTCAGCCCGGACTCACTATAAATACAGTAAGTAAGGT
AGCTGATTCACTGAGTCCCACCAGGAAACAAAAGCGATCGGTTCGACAAAACACCGCTAATAAATGTAAC
CCAGATCTTTACTATTGGACAGCTGTTGATGAGGGGGCAGCAGTAGGATTGGCATGGATTCCATATTTCG
GACCTGCAGCAGAAGGCATCTACATTGAGGGTGTAATGCATAATCAGAATGGGCTTATTTGCGGGCTACG
TCAGCTAGCCAATGAAACTACCCAGGCTCTTCAATTATTTCTGCGGGCCACAACAGAACTGAGGACTTAC
TCACTTCTTAACAGAAAAGCTATTGATTTTCTTCTTCAACGATGGGGAGGTACCTGTCGAATCCTAGGAC
CATCTTGTTGCATTGAGCCACATGATTGGACAAAAAATATTACTGATGAAATTAACCAAATTAAACATGA
CTTTATTGACAATCCCCTACCAGACCACGGAGATGATCTTAATCTATGGACAGGTTGGAGACAATGGATC
CCGGCTGGAATTGGGATTATTGGAGTTATAATTGCTATAATAGCCCTACTTTGTATATGTAAGATTTTGT
GTTGATTTATTCTGAGATCTGAGAGAGAAAAATCTCAGGGTTACTCTAAGGAGAAATATTATTTTTAAAA
TTTACTTGAATGCTGACCACTTATCTTAAATGAGCAATTAATAATATGTTTTTCTGCTTCTTTGCTTGAT
TTACAATATGATATTTCTCTTAATAATGATTAATATATTAAGAAAA

Protein Sequence : Show Sequence

>NP_690583.1 spike glycoprotein [Reston ebolavirus]
MGSGYQLLQLPRERFRKTSFLVWVIILFQRAISMPLGIVTNSTLKATEIDQLVCRDKLSSTSQLKSVGLN
LEGNGIATDVPSATKRWGFRSGVPPKVVSYEAGEWAENCYNLEIKKSDGSECLPLPPDGVRGFPRCRYVH
KVQGTGPCPGDLAFHKNGAFFLYDRLASTVIYRGTTFAEGVVAFLILSEPKKHFWKATPAHEPVNTTDDS
TSYYMTLTLSYEMSNFGGNESNTLFKVDNHTYVQLDRPHTPQFLVQLNETLRRNNRLSNSTGRLTWTLDP
KIEPDVGEWAFWETKKNFSQQLHGENLHFQIPSTHTNNSSDQSPAGTVQGKISYHPPANNSELVPTDSPP
VVSVLTAGRTEEMSTQGLTNGETITGFTANPMTTTIAPSPTMTSEVDNNVPSEQPNNTASIEDSPPSASN
ETIYHSEMDPIQGSNNSAQSPQTKTTPAPTTSPMTQDPQETANSSKPGTSPGSAAGPSQPGLTINTVSKV
ADSLSPTRKQKRSVRQNTANKCNPDLYYWTAVDEGAAVGLAWIPYFGPAAEGIYIEGVMHNQNGLICGLR
QLANETTQALQLFLRATTELRTYSLLNRKAIDFLLQRWGGTCRILGPSCCIEPHDWTKNITDEINQIKHD
FIDNPLPDHGDDLNLWTGWRQWIPAGIGIIGVIIAIIALLCICKILC

Molecule Role : Protective antigen
Related Vaccine(s): DNA vaccine expressing sGP , GP-VRP

4. GP from Sudan ebolavirus

Gene Name : GP from Sudan ebolavirus
Sequence Strain (Species/Organism) : Sudan ebolavirus
VO ID : VO_0010911
NCBI Gene ID : 3160774
NCBI Protein GI : 55770812
Locus Tag : SEVgp4
Genbank Accession : AY316199
Protein Accession : YP_138523
Taxonomy ID : 186540
Gene Starting Position : 5882
Gene Ending Position : 8240
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 6.35
Protein Weight : 71813.2
Protein Length : 676
Protein Note : forms dimers linked by disulfide bonds (parallel orientation); processed by furin to yield SGP and delta peptide

DNA Sequence : Show Sequence

>NC_006432.1:5882-8240 Sudan ebolavirus isolate Sudan virus/H.sapiens-tc/UGA/2000/Gulu-808892, complete genome
TGATGAAGATTAAGCCTGATGAAGGCCCAACCTTCATCTTTTTACCATAATCTTGTTCTCAGTACCATTT
GATAAGGGTACACTTGCCAATACGCCCCCATCCTAAGGGTCTCGCAATGGGGGGTCTTAGCCTACTCCAA
TTGCCCAGGGACAAATTTCGGAAAAGCTCTTTCTTTGTTTGGGTCATCATCTTATTCCAAAAGGCCTTTT
CCATGCCTTTGGGTGTTGTGACTAACAGCACTTTAGAAGTAACAGAGATTGACCAGCTAGTCTGCAAGGA
TCATCTTGCATCTACTGACCAGCTGAAATCAGTTGGTCTCAACCTCGAGGGGAGCGGAGTATCTACTGAT
ATCCCATCTGCAACAAAGCGTTGGGGCTTCAGATCTGGTGTTCCTCCCAAGGTGGTCAGCTATGAAGCGG
GAGAATGGGCTGAAAATTGCTACAATCTTGAAATAAAGAAGCCGGACGGGAGCGAATGCTTACCCCCACC
GCCAGATGGTGTCAGAGGCTTTCCAAGGTGCCGCTATGTTCACAAAGCCCAAGGAACCGGGCCCTGCCCA
GGTGACTACGCCTTTCACAAGGATGGAGCTTTCTTCCTCTATGACAGGCTGGCTTCAACTGTAATTTACA
GAGGAGTCAATTTTGCTGAGGGGGTAATTGCATTCTTGATATTGGCTAAACCAAAAGAAACGTTCCTTCA
GTCACCCCCCATTCGAGAGGCAGTAAACTACACTGAAAATACATCAAGTTATTATGCCACATCCTACTTG
GAGTATGAAATCGAAAATTTTGGTGCTCAACACTCCACGACCCTTTTCAAAATTGACAATAATACTTTTG
TTCGTCTGGACAGGCCCCACACGCCTCAGTTCCTTTTCCAGCTGAATGATACCATTCACCTTCACCAACA
GTTGAGTAATACAACTGGGAGACTAATTTGGACACTAGATGCTAATATCAATGCTGATATTGGTGAATGG
GCTTTTTGGGAAAATAAAAAAATCTCTCCGAACAACTACGTGGAGAAGAGCTGTCTTTCGAAGCTTTATC
GCTCAACGAGACAGAAGACGATGATGCGGCATCGTCGAGAATTACAAAGGGAAGAATCTCCGACCGGGCC
ACCAGGAAGTATTCGGACCTGGTTCCAAAGAATTCCCCTGGGATGGTTCCATTGCACATACCAGAAGGGG
AAACAACATTGCCGTCTCAGAATTCGACAGAAGGTCGAAGAGTAGGTGTGAACACTCAGGAGACCATTAC
AGAGACAGCTGCAACAATTATAGGCACTAACGGCAACCATATGCAGATCTCCACCATCGGGATAAGACCG
AGCTCCAGCCAAATCCCGAGTTCCTCACCGACCACGGCACCAAGCCCTGAGGCTCAGACCCCCACAACCC
ACACATCAGGTCCATCAGTGATGGCCACCGAGGAACCAACAACACCACCGGGAAGCTCCCCCGGCCCAAC
AACAGAAGCACCCACTCTCACCACCCCAGAAAATATAACAACAGCGGTTAAAACTGTCCTGCCACAGGAG
TCCACAAGCAACGGTCTAATAACTTCAACAGTAACAGGGATTCTTGGGAGTCTTGGGCTTCGAAAACGCA
GCAGAAGACAAACTAACACCAAAGCCACGGGTAAGTGCAATCCCAACTTACACTACTGGACTGCACAAGA
ACAACATAATGCTGCTGGGATTGCCTGGATCCCGTACTTTGGACCGGGTGCGGAAGGCATATACACTGAA
GGCCTGATGCATAACCAAAATGCCTTAGTCTGTGGACTTAGGCAACTTGCAAATGAAACAACTCAAGCTC
TGCAGCTTTTCTTAAGAGCCACAACGGAGCTGCGGACATATACCATACTCAATAGGAAGGCCATAGATTT
CCTTCTGCGACGATGGGGCGGGACATGCAGGATCCTGGGACCAGATTGTTGCATTGAGCCACATGATTGG
ACAAAAAACATCACTGATAAAATCAACCAAATCATCCATGATTTCATCGACAACCCCTTACCTAATCAGG
ATAATGATGATAATTGGTGGACGGGCTGGAGACAGTGGATCCCTGCAGGAATAGGCATTACTGGAATTAT
TATTGCAATTATTGCTCTTCTTTGCGTTTGCAAGCTGCTTTGCTGAATATCAATTTGAATCATCAATTTA
AGCTTGATACATTTCTAGCATTTTAAATTATAAACCGATACTGATACTTGAAAATCAGGCTAATGCCAAG
TTCTGTGCAAAACTTGAAAGTAGGTTTACAAAAATCCTTTGGACTGGAATGCTTTGATACTCTTTCTCAA
TACTATATAAGTTCCTTCCCAAGAATAATATTGATGAAGATTAAGAAAA

Protein Sequence : Show Sequence

>YP_138523.1 spike glycoprotein [Sudan ebolavirus]
MGGLSLLQLPRDKFRKSSFFVWVIILFQKAFSMPLGVVTNSTLEVTEIDQLVCKDHLASTDQLKSVGLNL
EGSGVSTDIPSATKRWGFRSGVPPKVVSYEAGEWAENCYNLEIKKPDGSECLPPPPDGVRGFPRCRYVHK
AQGTGPCPGDYAFHKDGAFFLYDRLASTVIYRGVNFAEGVIAFLILAKPKETFLQSPPIREAVNYTENTS
SYYATSYLEYEIENFGAQHSTTLFKIDNNTFVRLDRPHTPQFLFQLNDTIHLHQQLSNTTGRLIWTLDAN
INADIGEWAFWENKKNLSEQLRGEELSFEALSLNETEDDDAASSRITKGRISDRATRKYSDLVPKNSPGM
VPLHIPEGETTLPSQNSTEGRRVGVNTQETITETAATIIGTNGNHMQISTIGIRPSSSQIPSSSPTTAPS
PEAQTPTTHTSGPSVMATEEPTTPPGSSPGPTTEAPTLTTPENITTAVKTVLPQESTSNGLITSTVTGIL
GSLGLRKRSRRQTNTKATGKCNPNLHYWTAQEQHNAAGIAWIPYFGPGAEGIYTEGLMHNQNALVCGLRQ
LANETTQALQLFLRATTELRTYTILNRKAIDFLLRRWGGTCRILGPDCCIEPHDWTKNITDKINQIIHDF
IDNPLPNQDNDDNWWTGWRQWIPAGIGITGIIIAIIALLCVCKLLC

Molecule Role : Protective antigen
Molecule Role Annotation : Cynomolgus macaques were immunized with DNA/rAd5 vaccines expressing ZEBOV (GP) and SEBOV glycoprotein (GP) prior to lethal challenge with BEBOV. Vaccinated subjects developed robust, antigen-specific humoral and cellular immune responses against the GP from ZEBOV as well as cellular immunity against BEBOV GP, and immunized macaques were uniformly protected against lethal challenge with BEBOV (Hensley et al., 2010).
Related Vaccine(s): cAd3-EBO S , cAdVax-based bivalent ebola virus vaccine (Sudan and Zaire species) , CAdVax-ZEBOV/SEBOV , Ebola virus DNA vaccine DNA/rAd5 encoding ZEBOV and SEBOV antigens , Ebola virus DNA vaccine encoding ZEBOV GP and SEBOV GP , Ebola virus recombinant vector vaccine EBO7 encoding GP from SEBOV and ZEBOV

5. GP from Zaire ebolavirus

Gene Name : GP from Zaire ebolavirus
Sequence Strain (Species/Organism) : Zaire ebolavirus
VO ID : VO_0010910
NCBI Gene ID : 911829
NCBI Protein GI : 10313995
Locus Tag : ZEBOVgp4
Genbank Accession : AF272001
Protein Accession : NP_066246
Taxonomy ID : 186538
Gene Starting Position : 5899
Gene Ending Position : 8304
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 6.6
Protein Weight : 70989.69
Protein Length : 676
Protein Note : sGP secreted as a anti-parallel oriented homodimer

DNA Sequence : Show Sequence

>NC_002549.1:5899-8304 Zaire ebolavirus isolate Ebola virus/H.sapiens-tc/COD/1976/Yambuku-Mayinga, complete genome
CGATGAAGATTAAGCCGACAGTGAGCGTAATCTTCATCTCTCTTAGATTATTTGTTTTCCAGAGTAGGGG
TCGTCAGGTCCTTTTCAATCGTGTAACCAAAATAAACTCCACTAGAAGGATATTGTGGGGCAACAACACA
ATGGGCGTTACAGGAATATTGCAGTTACCTCGTGATCGATTCAAGAGGACATCATTCTTTCTTTGGGTAA
TTATCCTTTTCCAAAGAACATTTTCCATCCCACTTGGAGTCATCCACAATAGCACATTACAGGTTAGTGA
TGTCGACAAACTAGTTTGTCGTGACAAACTGTCATCCACAAATCAATTGAGATCAGTTGGACTGAATCTC
GAAGGGAATGGAGTGGCAACTGACGTGCCATCTGCAACTAAAAGATGGGGCTTCAGGTCCGGTGTCCCAC
CAAAGGTGGTCAATTATGAAGCTGGTGAATGGGCTGAAAACTGCTACAATCTTGAAATCAAAAAACCTGA
CGGGAGTGAGTGTCTACCAGCAGCGCCAGACGGGATTCGGGGCTTCCCCCGGTGCCGGTATGTGCACAAA
GTATCAGGAACGGGACCGTGTGCCGGAGACTTTGCCTTCCATAAAGAGGGTGCTTTCTTCCTGTATGATC
GACTTGCTTCCACAGTTATCTACCGAGGAACGACTTTCGCTGAAGGTGTCGTTGCATTTCTGATACTGCC
CCAAGCTAAGAAGGACTTCTTCAGCTCACACCCCTTGAGAGAGCCGGTCAATGCAACGGAGGACCCGTCT
AGTGGCTACTATTCTACCACAATTAGATATCAGGCTACCGGTTTTGGAACCAATGAGACAGAGTACTTGT
TCGAGGTTGACAATTTGACCTACGTCCAACTTGAATCAAGATTCACACCACAGTTTCTGCTCCAGCTGAA
TGAGACAATATATACAAGTGGGAAAAGGAGCAATACCACGGGAAAACTAATTTGGAAGGTCAACCCCGAA
ATTGATACAACAATCGGGGAGTGGGCCTTCTGGGAAACTAAAAAAACCTCACTAGAAAAATTCGCAGTGA
AGAGTTGTCTTTCACAGTTGTATCAAACGGAGCCAAAAACATCAGTGGTCAGAGTCCGGCGCGAACTTCT
TCCGACCCAGGGACCAACACAACAACTGAAGACCACAAAATCATGGCTTCAGAAAATTCCTCTGCAATGG
TTCAAGTGCACAGTCAAGGAAGGGAAGCTGCAGTGTCGCATCTAACAACCCTTGCCACAATCTCCACGAG
TCCCCAATCCCTCACAACCAAACCAGGTCCGGACAACAGCACCCATAATACACCCGTGTATAAACTTGAC
ATCTCTGAGGCAACTCAAGTTGAACAACATCACCGCAGAACAGACAACGACAGCACAGCCTCCGACACTC
CCTCTGCCACGACCGCAGCCGGACCCCCAAAAGCAGAGAACACCAACACGAGCAAGAGCACTGACTTCCT
GGACCCCGCCACCACAACAAGTCCCCAAAACCACAGCGAGACCGCTGGCAACAACAACACTCATCACCAA
GATACCGGAGAAGAGAGTGCCAGCAGCGGGAAGCTAGGCTTAATTACCAATACTATTGCTGGAGTCGCAG
GACTGATCACAGGCGGGAGAAGAACTCGAAGAGAAGCAATTGTCAATGCTCAACCCAAATGCAACCCTAA
TTTACATTACTGGACTACTCAGGATGAAGGTGCTGCAATCGGACTGGCCTGGATACCATATTTCGGGCCA
GCAGCCGAGGGAATTTACATAGAGGGGCTAATGCACAATCAAGATGGTTTAATCTGTGGGTTGAGACAGC
TGGCCAACGAGACGACTCAAGCTCTTCAACTGTTCCTGAGAGCCACAACTGAGCTACGCACCTTTTCAAT
CCTCAACCGTAAGGCAATTGATTTCTTGCTGCAGCGATGGGGCGGCACATGCCACATTCTGGGACCGGAC
TGCTGTATCGAACCACATGATTGGACCAAGAACATAACAGACAAAATTGATCAGATTATTCATGATTTTG
TTGATAAAACCCTTCCGGACCAGGGGGACAATGACAATTGGTGGACAGGATGGAGACAATGGATACCGGC
AGGTATTGGAGTTACAGGCGTTATAATTGCAGTTATCGCTTTATTCTGTATATGCAAATTTGTCTTTTAG
TTTTTCTTCAGATTGCTTCATGGAAAAGCTCAGCCTCAAATCAATGAAACCAGGATTTAATTATATGGAT
TACTTGAATCTAAGATTACTTGACAAATGATAATATAATACACTGGAGCTTTAAACATAGCCAATGTGAT
TCTAACTCCTTTAAACTCACAGTTAATCATAAACAAGGTTTGACATCAATCTAGTTATCTCTTTGAGAAT
GATAAACTTGATGAAGATTAAGAAAA

Protein Sequence : Show Sequence

>NP_066246.1 spike glycoprotein [Zaire ebolavirus]
MGVTGILQLPRDRFKRTSFFLWVIILFQRTFSIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNL
EGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHK
VSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPS
SGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPE
IDTTIGEWAFWETKKNLTRKIRSEELSFTVVSNGAKNISGQSPARTSSDPGTNTTTEDHKIMASENSSAM
VQVHSQGREAAVSHLTTLATISTSPQSLTTKPGPDNSTHNTPVYKLDISEATQVEQHHRRTDNDSTASDT
PSATTAAGPPKAENTNTSKSTDFLDPATTTSPQNHSETAGNNNTHHQDTGEESASSGKLGLITNTIAGVA
GLITGGRRTRREAIVNAQPKCNPNLHYWTTQDEGAAIGLAWIPYFGPAAEGIYIEGLMHNQDGLICGLRQ
LANETTQALQLFLRATTELRTFSILNRKAIDFLLQRWGGTCHILGPDCCIEPHDWTKNITDKIDQIIHDF
VDKTLPDQGDNDNWWTGWRQWIPAGIGVTGVIIAVIALFCICKFVF

Molecule Role : Protective antigen
Molecule Role Annotation : Cynomolgus macaques were immunized with DNA/rAd5 vaccines expressing ZEBOV (GP) and SEBOV glycoprotein (GP) prior to lethal challenge with BEBOV. Vaccinated subjects developed robust, antigen-specific humoral and cellular immune responses against the GP from ZEBOV as well as cellular immunity against BEBOV GP, and immunized macaques were uniformly protected against lethal challenge with BEBOV (Hensley et al., 2010).
Related Vaccine(s): cAd3-EBO S , cAdVax-based bivalent ebola virus vaccine (Sudan and Zaire species) , CAdVax-ZEBOV/SEBOV , Ebola virus DNA vaccine DNA/rAd5 encoding ZEBOV and SEBOV antigens , Ebola virus DNA vaccine EBOV GP , Ebola virus DNA vaccine encoding ZEBOV GP and SEBOV GP , Ebola virus DNA vaccine GP DNA , Ebola virus recombinant adenovirus vaccine AdC7-ZGP encoding GP , Ebola virus recombinant adenovirus vector vaccine ADV−GP/NP , Ebola virus recombinant rAD-GP encoding GP , Ebola virus recombinant vector vaccine Ad-CAGoptZGP encoding the envelope glycoprotein , Ebola virus recombinant vector vaccine Ad-CMVZGP encoding the glycoprotein , Ebola virus recombinant vector vaccine EBO7 encoding GP from SEBOV and ZEBOV , Ebola virus recombinant vector vaccine pVSVXN2∆G/ZEBOVsGP encoding GP , Ebola virus recombinant VSVΔG-GP encoding GP , rAd-GP (Ebola virus) , rVSV-EBOV

6. NP

Gene Name : NP
Sequence Strain (Species/Organism) : Zaire ebolavirus
VO ID : VO_0010912
NCBI Gene ID : 911830
NCBI Protein GI : 10314000
Locus Tag : ZEBOVgp1
Genbank Accession : AY142960
Protein Accession : NP_066243
Taxonomy ID : 186538
Gene Starting Position : 55
Gene Ending Position : 3025
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 4.77
Protein Weight : 79118.19
Protein Length : 739

DNA Sequence : Show Sequence

>NC_002549.1:55-3025 Zaire ebolavirus isolate Ebola virus/H.sapiens-tc/COD/1976/Yambuku-Mayinga, complete genome
TGAGGAAGATTAATAATTTTCCTCTCATTGAAATTTATATCGGAATTTAAATTGAAATTGTTACTGTAAT
CACACCTGGTTTGTTTCAGAGCCACATCACAAAGATAGAGAACAACCTAGGTCTCCGAAGGGAGCAAGGG
CATCAGTGTGCTCAGTTGAAAATCCCTTGTCAACACCTAGGTCTTATCACATCACAAGTTCCACCTCAGA
CTCTGCAGGGTGATCCAACAACCTTAATAGAAACATTATTGTTAAAGGACAGCATTAGTTCACAGTCAAA
CAAGCAAGATTGAGAATTAACCTTGGTTTTGAACTTGAACACTTAGGGGATTGAAGATTCAACAACCCTA
AAGCTTGGGGTAAAACATTGGAAATAGTTAAAAGACAAATTGCTCGGAATCACAAAATTCCGAGTATGGA
TTCTCGTCCTCAGAAAATCTGGATGGCGCCGAGTCTCACTGAATCTGACATGGATTACCACAAGATCTTG
ACAGCAGGTCTGTCCGTTCAACAGGGGATTGTTCGGCAAAGAGTCATCCCAGTGTATCAAGTAAACAATC
TTGAAGAAATTTGCCAACTTATCATACAGGCCTTTGAAGCAGGTGTTGATTTTCAAGAGAGTGCGGACAG
TTTCCTTCTCATGCTTTGTCTTCATCATGCGTACCAGGGAGATTACAAACTTTTCTTGGAAAGTGGCGCA
GTCAAGTATTTGGAAGGGCACGGGTTCCGTTTTGAAGTCAAGAAGCGTGATGGAGTGAAGCGCCTTGAGG
AATTGCTGCCAGCAGTATCTAGTGGAAAAAACATTAAGAGAACACTTGCTGCCATGCCGGAAGAGGAGAC
AACTGAAGCTAATGCCGGTCAGTTTCTCTCCTTTGCAAGTCTATTCCTTCCGAAATTGGTAGTAGGAGAA
AAGGCTTGCCTTGAGAAGGTTCAAAGGCAAATTCAAGTACATGCAGAGCAAGGACTGATACAATATCCAA
CAGCTTGGCAATCAGTAGGACACATGATGGTGATTTTCCGTTTGATGCGAACAAATTTTCTGATCAAATT
TCTCCTAATACACCAAGGGATGCACATGGTTGCCGGGCATGATGCCAACGATGCTGTGATTTCAAATTCA
GTGGCTCAAGCTCGTTTTTCAGGCTTATTGATTGTCAAAACAGTACTTGATCATATCCTACAAAAGACAG
AACGAGGAGTTCGTCTCCATCCTCTTGCAAGGACCGCCAAGGTAAAAAATGAGGTGAACTCCTTTAAGGC
TGCACTCAGCTCCCTGGCCAAGCATGGAGAGTATGCTCCTTTCGCCCGACTTTTGAACCTTTCTGGAGTA
AATAATCTTGAGCATGGTCTTTTCCCTCAACTATCGGCAATTGCACTCGGAGTCGCCACAGCACACGGGA
GTACCCTCGCAGGAGTAAATGTTGGAGAACAGTATCAACAACTCAGAGAGGCTGCCACTGAGGCTGAGAA
GCAACTCCAACAATATGCAGAGTCTCGCGAACTTGACCATCTTGGACTTGATGATCAGGAAAAGAAAATT
CTTATGAACTTCCATCAGAAAAAGAACGAAATCAGCTTCCAGCAAACAAACGCTATGGTAACTCTAAGAA
AAGAGCGCCTGGCCAAGCTGACAGAAGCTATCACTGCTGCGTCACTGCCCAAAACAAGTGGACATTACGA
TGATGATGACGACATTCCCTTTCCAGGACCCATCAATGATGACGACAATCCTGGCCATCAAGATGATGAT
CCGACTGACTCACAGGATACGACCATTCCCGATGTGGTGGTTGATCCCGATGATGGAAGCTACGGCGAAT
ACCAGAGTTACTCGGAAAACGGCATGAATGCACCAGATGACTTGGTCCTATTCGATCTAGACGAGGACGA
CGAGGACACTAAGCCAGTGCCTAATAGATCGACCAAGGGTGGACAACAGAAGAACAGTCAAAAGGGCCAG
CATATAGAGGGCAGACAGACACAATCCAGGCCAATTCAAAATGTCCCAGGCCCTCACAGAACAATCCACC
ACGCCAGTGCGCCACTCACGGACAATGACAGAAGAAATGAACCCTCCGGCTCAACCAGCCCTCGCATGCT
GACACCAATTAACGAAGAGGCAGACCCACTGGACGATGCCGACGACGAGACGTCTAGCCTTCCGCCCTTG
GAGTCAGATGATGAAGAGCAGGACAGGGACGGAACTTCCAACCGCACACCCACTGTCGCCCCACCGGCTC
CCGTATACAGAGATCACTCTGAAAAGAAAGAACTCCCGCAAGACGAGCAACAAGATCAGGACCACACTCA
AGAGGCCAGGAACCAGGACAGTGACAACACCCAGTCAGAACACTCTTTTGAGGAGATGTATCGCCACATT
CTAAGATCACAGGGGCCATTTGATGCTGTTTTGTATTATCATATGATGAAGGATGAGCCTGTAGTTTTCA
GTACCAGTGATGGCAAAGAGTACACGTATCCAGACTCCCTTGAAGAGGAATATCCACCATGGCTCACTGA
AAAAGAGGCTATGAATGAAGAGAATAGATTTGTTACATTGGATGGTCAACAATTTTATTGGCCGGTGATG
AATCACAAGAATAAATTCATGGCAATCCTGCAACATCATCAGTGAATGAGCATGGAACAATGGGATGATT
CAACCGACAAATAGCTAACATTAAGTAGTCAAGGAACGAAAACAGGAAGAATTTTTGATGTCTAAGGTGT
GAATTATTATCACAATAAAAGTGATTCTTATTTTTGAATTTAAAGCTAGCTTATTATTACTAGCCGTTTT
TCAAAGTTCAATTTGAGTCTTAATGCAAATAGGCGTTAAGCCACAGTTATAGCCATAATTGTAACTCAAT
ATTCTAACTAGCGATTTATCTAAATTAAATTACATTATGCTTTTATAACTTACCTACTAGCCTGCCCAAC
ATTTACACGATCGTTTTATAATTAAGAAAAA

Protein Sequence : Show Sequence

>NP_066243.1 nucleoprotein [Zaire ebolavirus]
MDSRPQKIWMAPSLTESDMDYHKILTAGLSVQQGIVRQRVIPVYQVNNLEEICQLIIQAFEAGVDFQESA
DSFLLMLCLHHAYQGDYKLFLESGAVKYLEGHGFRFEVKKRDGVKRLEELLPAVSSGKNIKRTLAAMPEE
ETTEANAGQFLSFASLFLPKLVVGEKACLEKVQRQIQVHAEQGLIQYPTAWQSVGHMMVIFRLMRTNFLI
KFLLIHQGMHMVAGHDANDAVISNSVAQARFSGLLIVKTVLDHILQKTERGVRLHPLARTAKVKNEVNSF
KAALSSLAKHGEYAPFARLLNLSGVNNLEHGLFPQLSAIALGVATAHGSTLAGVNVGEQYQQLREAATEA
EKQLQQYAESRELDHLGLDDQEKKILMNFHQKKNEISFQQTNAMVTLRKERLAKLTEAITAASLPKTSGH
YDDDDDIPFPGPINDDDNPGHQDDDPTDSQDTTIPDVVVDPDDGSYGEYQSYSENGMNAPDDLVLFDLDE
DDEDTKPVPNRSTKGGQQKNSQKGQHIEGRQTQSRPIQNVPGPHRTIHHASAPLTDNDRRNEPSGSTSPR
MLTPINEEADPLDDADDETSSLPPLESDDEEQDRDGTSNRTPTVAPPAPVYRDHSEKKELPQDEQQDQDH
TQEARNQDSDNTQSEHSFEEMYRHILRSQGPFDAVLYYHMMKDEPVVFSTSDGKEYTYPDSLEEEYPPWL
TEKEAMNEENRFVTLDGQQFYWPVMNHKNKFMAILQHHQ

Molecule Role : Protective antigen
Molecule Role Annotation : Guinea pigs infected with a single intranasal inoculation of HPIV3/EboGP-NP showed no apparent signs of disease yet developed a strong humoral response specific to the EV proteins. When these animals were challenged with an intraperitoneal injection of 10(3) PFU of EV, there were no outward signs of disease, no viremia or detectable EV antigen in the blood, and no evidence of infection in the spleen, liver, and lungs. In contrast, all of the control animals died or developed severe EV disease following challenge (Bukreyev et al., 2006).

7. NP from Zaire Ebola virus

Gene Name : NP from Zaire Ebola virus
Sequence Strain (Species/Organism) : Ebola virus - Mayinga, Zaire, 1976
NCBI Gene ID : 911830
NCBI Protein GI : 10314000
Locus Tag : ZEBOVgp1
Genbank Accession : AF086833
Protein Accession : NP_066243
Taxonomy ID : 128952
Gene Starting Position : 55
Gene Ending Position : 3025
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 4.77
Protein Weight : 79118.19
Protein Length : 739

DNA Sequence : Show Sequence

>gi|10313991:55-3025 Ebola virus - Mayinga, Zaire, 1976 strain Mayinga
TGAGGAAGATTAATAATTTTCCTCTCATTGAAATTTATATCGGAATTTAAATTGAAATTGTTACTGTAAT
CACACCTGGTTTGTTTCAGAGCCACATCACAAAGATAGAGAACAACCTAGGTCTCCGAAGGGAGCAAGGG
CATCAGTGTGCTCAGTTGAAAATCCCTTGTCAACACCTAGGTCTTATCACATCACAAGTTCCACCTCAGA
CTCTGCAGGGTGATCCAACAACCTTAATAGAAACATTATTGTTAAAGGACAGCATTAGTTCACAGTCAAA
CAAGCAAGATTGAGAATTAACCTTGGTTTTGAACTTGAACACTTAGGGGATTGAAGATTCAACAACCCTA
AAGCTTGGGGTAAAACATTGGAAATAGTTAAAAGACAAATTGCTCGGAATCACAAAATTCCGAGTATGGA
TTCTCGTCCTCAGAAAATCTGGATGGCGCCGAGTCTCACTGAATCTGACATGGATTACCACAAGATCTTG
ACAGCAGGTCTGTCCGTTCAACAGGGGATTGTTCGGCAAAGAGTCATCCCAGTGTATCAAGTAAACAATC
TTGAAGAAATTTGCCAACTTATCATACAGGCCTTTGAAGCAGGTGTTGATTTTCAAGAGAGTGCGGACAG
TTTCCTTCTCATGCTTTGTCTTCATCATGCGTACCAGGGAGATTACAAACTTTTCTTGGAAAGTGGCGCA
GTCAAGTATTTGGAAGGGCACGGGTTCCGTTTTGAAGTCAAGAAGCGTGATGGAGTGAAGCGCCTTGAGG
AATTGCTGCCAGCAGTATCTAGTGGAAAAAACATTAAGAGAACACTTGCTGCCATGCCGGAAGAGGAGAC
AACTGAAGCTAATGCCGGTCAGTTTCTCTCCTTTGCAAGTCTATTCCTTCCGAAATTGGTAGTAGGAGAA
AAGGCTTGCCTTGAGAAGGTTCAAAGGCAAATTCAAGTACATGCAGAGCAAGGACTGATACAATATCCAA
CAGCTTGGCAATCAGTAGGACACATGATGGTGATTTTCCGTTTGATGCGAACAAATTTTCTGATCAAATT
TCTCCTAATACACCAAGGGATGCACATGGTTGCCGGGCATGATGCCAACGATGCTGTGATTTCAAATTCA
GTGGCTCAAGCTCGTTTTTCAGGCTTATTGATTGTCAAAACAGTACTTGATCATATCCTACAAAAGACAG
AACGAGGAGTTCGTCTCCATCCTCTTGCAAGGACCGCCAAGGTAAAAAATGAGGTGAACTCCTTTAAGGC
TGCACTCAGCTCCCTGGCCAAGCATGGAGAGTATGCTCCTTTCGCCCGACTTTTGAACCTTTCTGGAGTA
AATAATCTTGAGCATGGTCTTTTCCCTCAACTATCGGCAATTGCACTCGGAGTCGCCACAGCACACGGGA
GTACCCTCGCAGGAGTAAATGTTGGAGAACAGTATCAACAACTCAGAGAGGCTGCCACTGAGGCTGAGAA
GCAACTCCAACAATATGCAGAGTCTCGCGAACTTGACCATCTTGGACTTGATGATCAGGAAAAGAAAATT
CTTATGAACTTCCATCAGAAAAAGAACGAAATCAGCTTCCAGCAAACAAACGCTATGGTAACTCTAAGAA
AAGAGCGCCTGGCCAAGCTGACAGAAGCTATCACTGCTGCGTCACTGCCCAAAACAAGTGGACATTACGA
TGATGATGACGACATTCCCTTTCCAGGACCCATCAATGATGACGACAATCCTGGCCATCAAGATGATGAT
CCGACTGACTCACAGGATACGACCATTCCCGATGTGGTGGTTGATCCCGATGATGGAAGCTACGGCGAAT
ACCAGAGTTACTCGGAAAACGGCATGAATGCACCAGATGACTTGGTCCTATTCGATCTAGACGAGGACGA
CGAGGACACTAAGCCAGTGCCTAATAGATCGACCAAGGGTGGACAACAGAAGAACAGTCAAAAGGGCCAG
CATATAGAGGGCAGACAGACACAATCCAGGCCAATTCAAAATGTCCCAGGCCCTCACAGAACAATCCACC
ACGCCAGTGCGCCACTCACGGACAATGACAGAAGAAATGAACCCTCCGGCTCAACCAGCCCTCGCATGCT
GACACCAATTAACGAAGAGGCAGACCCACTGGACGATGCCGACGACGAGACGTCTAGCCTTCCGCCCTTG
GAGTCAGATGATGAAGAGCAGGACAGGGACGGAACTTCCAACCGCACACCCACTGTCGCCCCACCGGCTC
CCGTATACAGAGATCACTCTGAAAAGAAAGAACTCCCGCAAGACGAGCAACAAGATCAGGACCACACTCA
AGAGGCCAGGAACCAGGACAGTGACAACACCCAGTCAGAACACTCTTTTGAGGAGATGTATCGCCACATT
CTAAGATCACAGGGGCCATTTGATGCTGTTTTGTATTATCATATGATGAAGGATGAGCCTGTAGTTTTCA
GTACCAGTGATGGCAAAGAGTACACGTATCCAGACTCCCTTGAAGAGGAATATCCACCATGGCTCACTGA
AAAAGAGGCTATGAATGAAGAGAATAGATTTGTTACATTGGATGGTCAACAATTTTATTGGCCGGTGATG
AATCACAAGAATAAATTCATGGCAATCCTGCAACATCATCAGTGAATGAGCATGGAACAATGGGATGATT
CAACCGACAAATAGCTAACATTAAGTAGTCAAGGAACGAAAACAGGAAGAATTTTTGATGTCTAAGGTGT
GAATTATTATCACAATAAAAGTGATTCTTATTTTTGAATTTAAAGCTAGCTTATTATTACTAGCCGTTTT
TCAAAGTTCAATTTGAGTCTTAATGCAAATAGGCGTTAAGCCACAGTTATAGCCATAATTGTAACTCAAT
ATTCTAACTAGCGATTTATCTAAATTAAATTACATTATGCTTTTATAACTTACCTACTAGCCTGCCCAAC
ATTTACACGATCGTTTTATAATTAAGAAAAA

Protein Sequence : Show Sequence

>gi|10314000|ref|NP_066243.1| NP gene product [Ebola virus - Mayinga, Zaire, 1976]
MDSRPQKIWMAPSLTESDMDYHKILTAGLSVQQGIVRQRVIPVYQVNNLEEICQLIIQAFEAGVDFQESA
DSFLLMLCLHHAYQGDYKLFLESGAVKYLEGHGFRFEVKKRDGVKRLEELLPAVSSGKNIKRTLAAMPEE
ETTEANAGQFLSFASLFLPKLVVGEKACLEKVQRQIQVHAEQGLIQYPTAWQSVGHMMVIFRLMRTNFLI
KFLLIHQGMHMVAGHDANDAVISNSVAQARFSGLLIVKTVLDHILQKTERGVRLHPLARTAKVKNEVNSF
KAALSSLAKHGEYAPFARLLNLSGVNNLEHGLFPQLSAIALGVATAHGSTLAGVNVGEQYQQLREAATEA
EKQLQQYAESRELDHLGLDDQEKKILMNFHQKKNEISFQQTNAMVTLRKERLAKLTEAITAASLPKTSGH
YDDDDDIPFPGPINDDDNPGHQDDDPTDSQDTTIPDVVVDPDDGSYGEYQSYSENGMNAPDDLVLFDLDE
DDEDTKPVPNRSTKGGQQKNSQKGQHIEGRQTQSRPIQNVPGPHRTIHHASAPLTDNDRRNEPSGSTSPR
MLTPINEEADPLDDADDETSSLPPLESDDEEQDRDGTSNRTPTVAPPAPVYRDHSEKKELPQDEQQDQDH
TQEARNQDSDNTQSEHSFEEMYRHILRSQGPFDAVLYYHMMKDEPVVFSTSDGKEYTYPDSLEEEYPPWL
TEKEAMNEENRFVTLDGQQFYWPVMNHKNKFMAILQHHQ

Molecule Role : Other
Related Vaccine(s): Ebola virus EBOV NP , Ebola virus recombinant adenovirus vector vaccine ADV−GP/NP , rCMV- EBOV , rVEE-Ebola-NP , rVSV- SEBOV-GP and -VP40

8. pagA

Gene Name : pagA
Sequence Strain (Species/Organism) : Bacillus anthracis
NCBI Protein GI : BAD14937
Other Database IDs : CDD:281492
Taxonomy ID : 1392
Protein Name : pagA
Protein pI : 6.4
Protein Weight : 24803.18
Protein Length : 302
Protein Note : a partial cDNA fragment of pag gene generated a Eco RV site in the middle of the sequence by the overlap extension PCR

Protein Sequence : Show Sequence

>BAD14937.1 pagA, partial (plasmid) [Bacillus anthracis]
KRSTSAGPTVPDRDNDGIPDSLEVEGYTVDVKNKRTFLSPWISNIHEKKGLTKYKSSPEKWSTASDPYSD
FEKVTGRIDKNVSPEARHPLVAAYPIVHVDIENIILSKNEDQSTQNTDSQTRTISKNTSTSRTHTSEVHG
NAEVHASFFDIGGSVSAGFSNSNSSTVAIDHSLSLAGERTWAETMGLNTADTARLNADIRYVNTGTAPIY
NVLPTTSLVLGKNQTLATIKAKENQLSQIL

Molecule Role : Protective antigen
Molecule Role Annotation : (Garufi et al., 2012)

9. SGP

Gene Name : SGP
Sequence Strain (Species/Organism) : Sudan ebolavirus strain Boniface
NCBI Nucleotide GI : 1041223
NCBI Protein GI : 1041225
Protein Accession : AAB37096.1
Other Database IDs : CDD:279888
CDD:197367
Taxonomy ID : 186540
Gene Strand (Orientation) : ?
Protein Name : virion spike glycoprotein precursor
Protein pI : 5.67
Protein Weight : 72335.57
Protein Length : 754
Protein Note : subtype: Sudan

DNA Sequence : Show Sequence

>gi|1041223|gb|U28134.1|EVU28134 Sudan Ebola virus strain Boniface virion spike glycoprotein (SP) gene, complete cds, and small/secreted glycoprotein precursor (SGP) gene, complete cds
ATTTGATGAAGATTAAGCCTGATTAAGGCCCAACCTTCATCTTTTTACCATAATCTTGTTCTCAATACCA
TTTAATAGGGGTATACTTGCCAAAGCGCCCCCATCCTCAGGATCTCGCAATGGAGGGTCTTAGCCTACTC
CAATTGCCCAGAGATAAATTTCGAAAAAGCTCTTTCTTTGTTTGGGTCATCATCTTATTTCAAAAGGCCT
TTTCCATGCCTTTGGGTGTTGTGACCAACAGCACTTTAGAAGTAACAGAGATTGACCAGCTAGTCTGCAA
GGATCATCTTGCATCAACTGACCAGCTGAAATCAGTTGGTCTCAACCTCGAGGGGAGCGGAGTATCTACT
GATATCCCATCTGCGACAAAGCGTTGGGGCTTCAGATCTGGTGTGCCTCCCCAAGTGGTCAGCTATGAAG
CAGGAGAATGGGCTGAAAATTGCTACAATCTTGAAATAAAGAAACCGGACGGGAGCGAATGCTTACCCCC
ACCGCCGGATGGTGTCAGAGGCTTTCCAAGGTGCCGCTATGTTCACAAAGCCCAAGGAACCGGGCCCTGC
CCGGGTGACTATGCCTTTCACAAGGATGGAGCTTTCTTCCTCTATGACAGGCTGGCTTCAACTGTAATTT
ACAGAGGAGTCAATTTTGCTGAGGGGGTAATCGCATTCTTGATATTGGCTAAACCAAAGGAAACGTTCCT
TCAATCACCCCCCATTCGAGAGGCAGCAAACTACACTGAAAATACATCAAGTTACTATGCCACATCCTAC
TTGGAGTACGAAATCGAAAATTTTGGTGCTCAACACTCCACGACCCTTTTCAAAATTAACAATAATACTT
TTGTTCTTCTGGACAGGCCCCACACGCCTCAGTTCCTTTTCCAGCTGAATGATACCATTCAACTTCACCA
ACAGTTGAGCAACACAACTGGGAAACTAATTTGGACACTAGATGCTAATATCAATGCTGATATTGGTGAA
TGGGCTTTTTGGGAAAATAAAAAAATCTCTCCGAACAACTACGTGGAGAAGAGCTGTCTTTCGAAACTTT
ATCGCTCAACGAGACAGAAGACGATGATGCGACATCGTCGAGAACTACAAAGGGAAGAATCTCCGACCGG
GCCACCAGGAAGTATTCGGACCTGGTTCCAAAGGATTCCCCTGGGATGGTTTCATTGCACGTACCAGAAG
GGGAAACAACATTGCCGTCTCAGAATTCGACAGAAGGTCGAAGAGTAGATGTGAATACTCAGGAAACTAT
CACAGAGACAACTGCAACAATCATAGGCACTAACGGTAACAACATGCAGATCTCCACCATCGGGACAGGA
CTGAGCTCCAGCCAAATCCTGAGTTCCTCACCGACCATGGCACCAAGCCCTGAGACTCAGACCTCCACAA
CCTACACACCAAAACTACCAGTGATGACCACCGAGGAATCAACAACACCACCGAGAAACTCTCCTGGCTC
AACAACAGAAGCACCCACTCTCACCACCCCAGAGAATATAACAACAGCGGTTAAAACTGTTTGGCCACAA
GAGTCCACAAGCAACGGTCTAATAACTTCAACAGTAACAGGGATTCTTGGGAGCCTTGGACTTCGAAAAC
GCAGCAGAAGACAAGTTAACACCAGGGCCACGGGTAAATGCAATCCCAACTTACACTACTGGACTGCACA
AGAACAACATAATGCTGCTGGGATTGCCTGGATCCCGTACTTTGGACCGGGTGCAGAAGGCATATACACT
GAAGGCCTTATGCACAACCAAAATGCCTTAGTCTGTGGACTCAGACAACTTGCAAATGAAACAACTCAAG
CTCTGCAGCTTTTCTTAAGGGCCACGACGGAGCTGCGGACATATACCATACTCAATAGGAAGGCCATAGA
TTTCCTTCTGCGACGATGGGGCGGGACATGTAGGATCCTGGGACCAGATTGTTGCATTGAGCCACATGAT
TGGACCAAAAACATCACTGATAAAATCAACCAAATCATCCATGATTTCATCGACAACCCTTTACCCAATC
AGGATAATGATGATAATTGGTGGACGGGCTGGAGACAGTGGATCCCTGCAGGAATAGGCATTACTGGAAT
TATTATTGCAATCATTGCTCTTCTTTGCGTCTGCAAGCTGCTTTGTTGAATATCAACTTGAATCATTAAT
TTAAAGTTGATACATTTCTAACATTATAAATTATAATCTGATATTAATACTTGAAAATAAGGCTAATGCC
AAATTCTGTGCCAAACTTGAAAGTAGGTTTACCAAAATCCTTTGAACTGGAATGCTTTAATGCTCTTTCT
CAATACTATATAAGTTCCTTCCCAAAATAATATTGATGAAGATTAAGAAAAA

Protein Sequence : Show Sequence

>AAB37096.1 virion spike glycoprotein precursor [Sudan ebolavirus]
MEGLSLLQLPRDKFRKSSFFVWVIILFQKAFSMPLGVVTNSTLEVTEIDQLVCKDHLASTDQLKSVGLNL
EGSGVSTDIPSATKRWGFRSGVPPQVVSYEAGEWAENCYNLEIKKPDGSECLPPPPDGVRGFPRCRYVHK
AQGTGPCPGDYAFHKDGAFFLYDRLASTVIYRGVNFAEGVIAFLILAKPKETFLQSPPIREAANYTENTS
SYYATSYLEYEIENFGAQHSTTLFKINNNTFVLLDRPHTPQFLFQLNDTIQLHQQLSNTTGKLIWTLDAN
INADIGEWAFWENKKNLSEQLRGEELSFETLSLNETEDDDATSSRTTKGRISDRATRKYSDLVPKDSPGM
VSLHVPEGETTLPSQNSTEGRRVDVNTQETITETTATIIGTNGNNMQISTIGTGLSSSQILSSSPTMAPS
PETQTSTTYTPKLPVMTTEESTTPPRNSPGSTTEAPTLTTPENITTAVKTVWPQESTSNGLITSTVTGIL
GSLGLRKRSRRQVNTRATGKCNPNLHYWTAQEQHNAAGIAWIPYFGPGAEGIYTEGLMHNQNALVCGLRQ
LANETTQALQLFLRATTELRTYTILNRKAIDFLLRRWGGTCRILGPDCCIEPHDWTKNITDKINQIIHDF
IDNPLPNQDNDDNWWTGWRQWIPAGIGITGIIIAIIALLCVCKLLC

Molecule Role : Protective antigen

10. VP24

Gene Name : VP24
Sequence Strain (Species/Organism) : Ebola virus
NCBI Protein GI : SCD11538
Other Database IDs : CDD:283933
Taxonomy ID : 1570291
Protein Name : VP24
Protein pI : 9.73
Protein Weight : 27754.06
Protein Length : 287
Protein Note : monopartite

Protein Sequence : Show Sequence

>SCD11538.1 VP24 [Ebola virus]
MAKATGRYNLISPKKDLEKGVVLSDLCNFLVSQTIQGWKVYWAGIEFDVTHKGMALLHRLKTNDFAPAWS
MTRNLFPHLFQNPNSTIESPLWALRVILAAGIQDQLIDQSLIEPLAGALGLISDWLLTTNTNHFNMRTQR
VKEQLSLKMLSLIRSNILKFINKLDALHVVNYNGLLSSIEIGTQNHTIIITRTNMGFLVELQEPDKSAMN
RKKPGPAKFSLLHESTLKAFTQGSSTRMQSLILEFNSSLAI

Molecule Role : Protective antigen
Molecule Role Annotation :

11. VP24 from Reston ebolavirus

Gene Name : VP24 from Reston ebolavirus
Sequence Strain (Species/Organism) : Reston ebolavirus
VO ID : VO_0010860
NCBI Gene ID : 955193
NCBI Protein GI : 22789228
Locus Tag : REBOVgp7
Genbank Accession : AF522874
Protein Accession : NP_690586
Taxonomy ID : 186539
Gene Starting Position : 9831
Gene Ending Position : 11480
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 10.16
Protein Weight : 26779.18
Protein Length : 251

DNA Sequence : Show Sequence

>NC_004161.1:9831-11480 Reston ebolavirus isolate Reston virus/M.fascicularis-tc/USA/1989/Philippines89-Pennsylvania, complete genome
GGATGAAGATTAATTGCGGAGGAATCAGGAATTCAACTTTAGTTCCTTAAGGCCTCGTCCGAATCTTCAT
CAGTTCGTAAGTTCTTTTATAGAAGTCATTAGCTTCTAAGGTGATTATATTTTAGTATTAAATTTTGCTA
ATTGCTTGCTATAAAGTTGAAATGTCTAATGCTTAAATGAACACTTTTTTGAAGCTGACATACGAATACA
TCATATCATATGAAAACATCGCAATTAGAGCGTCCTTGAAGTCTGGCATTGACAGTCACCAGGCTGTTCT
CAGTAGTCTGTCCTTGGAAGCTCTTGGGGAGACAAAAAGAGGTCCCAGAGAGTCCCAACAGGTTGGCATA
AGGTCATTAACACCAGCATAGTCGGCTCGACCAAGACTGTAAGCGAGTCGATTTCAACTAAAAAGATTAT
TTCTTGTTGTTTAAACAAATTCCTTTTGTGTGAGACATCCTCAAGGCACAAGATGGCTAAAGCCACAGGC
CGATACAATCTCGTGCCCCCAAAGAAAGATATGGAAAAGGGAGTGATTTTTAGTGATCTTTGTAATTTCT
TGATTACTCAAACCCTGCAAGGTTGGAAGGTTTATTGGGCAGGAATTGAGTTTGATGTAAGTCAAAAAGG
CATGGCTCTTCTGACAAGACTCAAAACAAATGACTTTGCTCCTGCCTGGGCGATGACAAGAAATCTTTTC
CCACATCTGTTCCAGAACCCAAATTCGGTTATTCAATCTCCCATCTGGGCTTTGAGGGTAATTTTGGCAG
CCGGATTGCAGGATCAGTTGTTAGACCATTCATTGGTTGAGCCATTGACAGGGGCTCTCGGTCTAATTTC
TGATTGGCTCCTAACTACAACGTCAACACATTTCAATCTTCGTACTAGAAGCGTAAAGGACCAGCTTAGT
CTTCGTATGTTATCTTTGATCAGGTCAAACATCTTGCAGTTCATCAACAAGCTTGACGCCCTGCATGTTG
TCAATTACAATGGTTTACTCAGTAGTATTGAGATCGGGACTTCTACACACACAATCATTATAACTCGTAC
AAATATGGGTTTTCTCGTGGAAGTTCAAGAGCCTGACAAATCAGCTATGAATTCTAAGCGCCCAGGACCA
GTCAAGTTCTCATTACTTCATGAGTCTGCCTTCAAACCTTTCACTCGTGTTCCACAATCTGGGATGCAAT
CATTAATAATGGAGTTCAACAGTTTGTTGGCAATTTAACAAGGTAATCTTAAAATAAGTACATGAATGAG
AATTAGTTGTGGGTCTTATCTAGCATTGTTGAGTTAACTATCTAATCTATTTTCGCTAATTGCATTGAGC
ACTGCTAATAGGTTTGTATCACGTTAAAGATTTAGAGTGTATGAATTGTGCAGATTTAAACTTGGGTTTT
GCCTTATGCTTCATAGGTGGTCTTTTTGAAATGGAGATTATCAGCATTTCTTAAATGGGAGGAGTTAGCA
ATCAGAAATTGGAGATAAATGGACATCGGGATAGAACAATGCCTAACTATTGGGCGGCTTCCATTTTTAC
ATGTGTATATAACCAATCTTTTCCTATCTTTGCTTATATTGGTGTAACTTTATTTTAATAACATGTCAAT
GCTATACTGTTAAGAGAAGGTCTGAGGAAGATTAAGAAAA

Protein Sequence : Show Sequence

>NP_690586.1 membrane-associated protein [Reston ebolavirus]
MAKATGRYNLVPPKKDMEKGVIFSDLCNFLITQTLQGWKVYWAGIEFDVSQKGMALLTRLKTNDFAPAWA
MTRNLFPHLFQNPNSVIQSPIWALRVILAAGLQDQLLDHSLVEPLTGALGLISDWLLTTTSTHFNLRTRS
VKDQLSLRMLSLIRSNILQFINKLDALHVVNYNGLLSSIEIGTSTHTIIITRTNMGFLVEVQEPDKSAMN
SKRPGPVKFSLLHESAFKPFTRVPQSGMQSLIMEFNSLLAI

Molecule Role : Protective antigen

12. VP24 from Zaire ebolavirus

Gene Name : VP24 from Zaire ebolavirus
Sequence Strain (Species/Organism) : Zaire ebolavirus
VO ID : VO_0010866
NCBI Gene ID : 911828
NCBI Protein GI : 10313998
Locus Tag : ZEBOVgp6
Genbank Accession : AY142960
Protein Accession : NP_066250
Taxonomy ID : 186538
Gene Starting Position : 9884
Gene Ending Position : 11517
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 10.1
Protein Weight : 27225.61
Protein Length : 251

DNA Sequence : Show Sequence

>NC_002549.1:9884-11517 Zaire ebolavirus isolate Ebola virus/H.sapiens-tc/COD/1976/Yambuku-Mayinga, complete genome
TGATGAAGATTAATGCGGAGGTCTGATAAGAATAAACCTTATTATTCAGATTAGGCCCCAAGAGGCATTC
TTCATCTCCTTTTAGCAAAGTACTATTTCAGGGTAGTCCAATTAGTGGCACGTCTTTTAGCTGTATATCA
GTCGCCCCTGAGATACGCCACAAAAGTGTCTCTAAGCTAAATTGGTCTGTACACATCCCATACATTGTAT
TAGGGGCAATAATATCTAATTGAACTTAGCCGTTTAAAATTTAGTGCATAAATCTGGGCTAACACCACCA
GGTCAACTCCATTGGCTGAAAAGAAGCTTACCTACAACGAACATCACTTTGAGCGCCCTCACAATTAAAA
AATAGGAACGTCGTTCCAACAATCGAGCGCAAGGTTTCAAGGTTGAACTGAGAGTGTCTAGACAACAAAA
TATTGATACTCCAGACACCAAGCAAGACCTGAGAAAAAACCATGGCTAAAGCTACGGGACGATACAATCT
AATATCGCCCAAAAAGGACCTGGAGAAAGGGGTTGTCTTAAGCGACCTCTGTAACTTCTTAGTTAGCCAA
ACTATTCAGGGGTGGAAGGTTTATTGGGCTGGTATTGAGTTTGATGTGACTCACAAAGGAATGGCCCTAT
TGCATAGACTGAAAACTAATGACTTTGCCCCTGCATGGTCAATGACAAGGAATCTCTTTCCTCATTTATT
TCAAAATCCGAATTCCACAATTGAATCACCGCTGTGGGCATTGAGAGTCATCCTTGCAGCAGGGATACAG
GACCAGCTGATTGACCAGTCTTTGATTGAACCCTTAGCAGGAGCCCTTGGTCTGATCTCTGATTGGCTGC
TAACAACCAACACTAACCATTTCAACATGCGAACACAACGTGTCAAGGAACAATTGAGCCTAAAAATGCT
GTCGTTGATTCGATCCAATATTCTCAAGTTTATTAACAAATTGGATGCTCTACATGTCGTGAACTACAAC
GGATTGTTGAGCAGTATTGAAATTGGAACTCAAAATCATACAATCATCATAACTCGAACTAACATGGGTT
TTCTGGTGGAGCTCCAAGAACCCGACAAATCGGCAATGAACCGCATGAAGCCTGGGCCGGCGAAATTTTC
CCTCCTTCATGAGTCCACACTGAAAGCATTTACACAAGGATCCTCGACACGAATGCAAAGTTTGATTCTT
GAATTTAATAGCTCTCTTGCTATCTAACTAAGGTAGAATACTTCATATTGAGCTAACTCATATATGCTGA
CTCAATAGTTATCTTGACATCTCTGCTTTCATAATCAGATATATAAGCATAATAAATAAATACTCATATT
TCTTGATAATTTGTTTAACCACAGATAAATCCTCACTGTAAGCCAGCTTCCAAGTTGACACCCTTACAAA
AACCAGGACTCAGAATCCCTCAAACAAGAGATTCCAAGACAACATCATAGAATTGCTTTATTATATGAAT
AAGCATTTTATCACCAGAAATCCTATATACTAAATGGTTAATTGTAACTGAACCCGCAGGTCACATGTGT
TAGGTTTCACAGATTCTATATATTACTAACTCTATACTCGTAATTAACATTAGATAAGTAGATTAAGAAA
AAAGCCTGAGGAAGATTAAGAAAA

Protein Sequence : Show Sequence

>NP_066250.1 membrane-associated protein [Zaire ebolavirus]
MAKATGRYNLISPKKDLEKGVVLSDLCNFLVSQTIQGWKVYWAGIEFDVTHKGMALLHRLKTNDFAPAWS
MTRNLFPHLFQNPNSTIESPLWALRVILAAGIQDQLIDQSLIEPLAGALGLISDWLLTTNTNHFNMRTQR
VKEQLSLKMLSLIRSNILKFINKLDALHVVNYNGLLSSIEIGTQNHTIIITRTNMGFLVELQEPDKSAMN
RMKPGPAKFSLLHESTLKAFTQGSSTRMQSLILEFNSSLAI

Molecule Role : Protective antigen
Molecule Role Annotation : VP24 expressed from alphavirus replicons induced a protective immune response in BALB/c mice when challenged with Ebola virus Zaire (Wilson et al., 2001).
Additional Molecule Role : Protective antigen
Related Vaccine(s): VRP expressing VP24

13. VP30

Gene Name : VP30
Sequence Strain (Species/Organism) : Ebola virus
NCBI Protein GI : SCD11537
Other Database IDs : CDD:314419
Taxonomy ID : 1570291
Protein Name : VP30
Protein pI : 7.74
Protein Weight : 31662.28
Protein Length : 325
Protein Note : monopartite

Protein Sequence : Show Sequence

>SCD11537.1 VP30 [Ebola virus]
MEASYERGRPRAARQHSRDGHDHHVRARSSSRENYRGEYRQSRSASQVRVPTVFHKKRVEPLTVPPAPKD
ICPTLKKGFLCDSSFCKKDHQLESLTDRELLLLIARKTCGSVEQQLNITAPKDSRLANPTADDFQQEEGP
KITLLTLIKTAEHWARQDIRTIEDSKLRALLTLCAVMTRKFSKSQLSLLCETHLRREGLGQDQAEPVLEV
YQRLHSDKGGSFEAALWQQWDRQSLIMFITAFLNIALQLPCESSAVVVSGLRTLVPQSDNEEASTNPGTC
SWSDEGTP

Molecule Role : Protective antigen
Molecule Role Annotation :

14. VP30 from Reston ebolavirus

Gene Name : VP30 from Reston ebolavirus
Sequence Strain (Species/Organism) : Reston ebolavirus
VO ID : VO_0010862
NCBI Gene ID : 955196
NCBI Protein GI : 22789227
Locus Tag : REBOVgp6
Genbank Accession : AF522874
Protein Accession : NP_690585
Taxonomy ID : 186539
Gene Starting Position : 8261
Gene Ending Position : 9700
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 8.3
Protein Weight : 31307.9
Protein Length : 287

DNA Sequence : Show Sequence

>NC_004161.1:8261-9700 Reston ebolavirus isolate Reston virus/M.fascicularis-tc/USA/1989/Philippines89-Pennsylvania, complete genome
TGACGAAGATTAAAGGAGAGGATCGTTAACGGGAAAACCTCCCATCTCGTTCGTCGAAGCCACGTTGGTG
GTGCTTGCAGCTGAGAACAACTCCAGAGATTGTAGGTAGAAAGGACCAACATTTATAGGTAGGGGTCAGA
AAGCAACAATAACCATAAAAGGAGAGCCTGACATTGCTATTTAATATCCTAGAACCTGATTTCTAGGTTC
TAGCTTTAAAATCCGGATGATGGAGCATTCAAGAGAACGGGGTAGATCTAGCAACATGCGACATAATAGC
CGGGAACCATACGAAAATCCATCAAGGTCTCGCTCATTATCTCGGGACCCTAATCAGGTTGATCGTAGAC
AGCCTCGAAGTGCATCCCAAATTCGTGTTCCGAATCTGTTCCATCGGAAAAAGACTGATGCACTCATAGT
TCCTCCGGCTCCTAAAGATATATGCCCAACACTCAAAAAAGGATTCCTCTGCGATAGCAAATTTTGCAAA
AAAGATCACCAATTGGATAGCTTAAATGATCATGAATTACTACTGCTAATTGCAAGAAGAACATGTGGAA
TTATCGAGAGCAATTCGCAGATTACATCCCCAAAAGATATGCGGTTAGCGAATCCAACAGCTGAAGACTT
CTCACAAGGTAATAGTCCTAAATTAACACTTGCAGTCCTTCTTCAAATTGCTGAACATTGGGCAACCAGA
GACCTAAGGCAAATTGAGGACTCTAAACTTAGAGCTCTTTTAACCCTTTGTGCCGTATTAACAAGGAAAT
TTTCTAAATCCCAACTGGGTCTTCTATGTGAGACCCACCTACGGCATGAGGGCCTCGGACAGGACCAAGC
TGATTCTGTATTAGAGGTCTACCAAAGACTCCACAGTGATAAAGGAGGGAATTTTGAGGCTGCCCTGTGG
CAACAATGGGACCGACAGTCATTAATAATGTTCATCTCTGCTTTTCTCAACATTGCTCTCCAGATACCTT
GTGAAAGTTCTAGTGTCGTAGTCTCAGGTCTTGCCACATTGTACCCAGCACAAGACAATTCTACACCATC
CGAGGCAACTAATGATACCACCTGGTCAAGTACAGTTGAATAGAAAACCACTGGAGCTATTTTTCCACGA
TTGCTCTCAGTCAATAAATTAATATAGATATAATACGACTTCGGTGTGCAATTGTCAAGAGTTCCATTTA
GTAATAATGATTCTTAAAACAATCTACTATCGCAATTATCGATGGATCTACCCTATTTGACGGTACATGA
CTTGAATGTAATAAGGTAAGTTGGTATCTGAGGTATTTTGTCTAGAGTATACTCAAAATCGTATGTCTAG
CAAATTATCAATAGCAAAGTTAAATTCTCCTAACCTCATATTTTGATCAAGTAATCATGATTTTATGATA
ATTCTTTTCAGATTATCGGTTTAATCTTTATTAAGAAAAA

Protein Sequence : Show Sequence

>NP_690585.1 minor nucleoprotein [Reston ebolavirus]
MEHSRERGRSSNMRHNSREPYENPSRSRSLSRDPNQVDRRQPRSASQIRVPNLFHRKKTDALIVPPAPKD
ICPTLKKGFLCDSKFCKKDHQLDSLNDHELLLLIARRTCGIIESNSQITSPKDMRLANPTAEDFSQGNSP
KLTLAVLLQIAEHWATRDLRQIEDSKLRALLTLCAVLTRKFSKSQLGLLCETHLRHEGLGQDQADSVLEV
YQRLHSDKGGNFEAALWQQWDRQSLIMFISAFLNIALQIPCESSSVVVSGLATLYPAQDNSTPSEATNDT
TWSSTVE

Molecule Role : Protective antigen

15. VP30 from Zaire ebolavirus

Gene Name : VP30 from Zaire ebolavirus
Sequence Strain (Species/Organism) : Zaire ebolavirus
VO ID : VO_0010867
NCBI Gene ID : 911826
NCBI Protein GI : 10313997
Locus Tag : ZEBOVgp5
Genbank Accession : AF272001
Protein Accession : NP_066249
3D structure: PDB ID : 2I8B
Taxonomy ID : 186538
Gene Starting Position : 8287
Gene Ending Position : 9739
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 8.23
Protein Weight : 31130.81
Protein Length : 288

DNA Sequence : Show Sequence

>NC_002549.1:8287-9739 Zaire ebolavirus isolate Ebola virus/H.sapiens-tc/COD/1976/Yambuku-Mayinga, complete genome
TGATGAAGATTAAGAAAAAGGTAATCTTTCGATTATCTTTAATCTTCATCCTTGATTCTACAATCATGAC
AGTTGTCTTTAGTGACAAGGGAAAGAAGCCTTTTTATTAAGTTGTAATAATCAGATCTGCGAACCGGTAG
AGTTTAGTTGCAACCTAACACACATAAAGCATTGGTCAAAAAGTCAATAGAAATTTAAACAGTGAGTGGA
GACAACTTTTAAATGGAAGCTTCATATGAGAGAGGACGCCCACGAGCTGCCAGACAGCATTCAAGGGATG
GACACGACCACCATGTTCGAGCACGATCATCATCCAGAGAGAATTATCGAGGTGAGTACCGTCAATCAAG
GAGCGCCTCACAAGTGCGCGTTCCTACTGTATTTCATAAGAAGAGAGTTGAACCATTAACAGTTCCTCCA
GCACCTAAAGACATATGTCCGACCTTGAAAAAAGGATTTTTGTGTGACAGTAGTTTTTGCAAAAAAGATC
ACCAGTTGGAGAGTTTAACTGATAGGGAATTACTCCTACTAATCGCCCGTAAGACTTGTGGATCAGTAGA
ACAACAATTAAATATAACTGCACCCAAGGACTCGCGCTTAGCAAATCCAACGGCTGATGATTTCCAGCAA
GAGGAAGGTCCAAAAATTACCTTGTTGACACTGATCAAGACGGCAGAACACTGGGCGAGACAAGACATCA
GAACCATAGAGGATTCAAAATTAAGAGCATTGTTGACTCTATGTGCTGTGATGACGAGGAAATTCTCAAA
ATCCCAGCTGAGTCTTTTATGTGAGACACACCTAAGGCGCGAGGGGCTTGGGCAAGATCAGGCAGAACCC
GTTCTCGAAGTATATCAACGATTACACAGTGATAAAGGAGGCAGTTTTGAAGCTGCACTATGGCAACAAT
GGGACCGACAATCCCTAATTATGTTTATCACTGCATTCTTGAATATTGCTCTCCAGTTACCGTGTGAAAG
TTCTGCTGTCGTTGTTTCAGGGTTAAGAACATTGGTTCCTCAATCAGATAATGAGGAAGCTTCAACCAAC
CCGGGGACATGCTCATGGTCTGATGAGGGTACCCCTTAATAAGGCTGACTAAAACACTATATAACCTTCT
ACTTGATCACAATACTCCGTATACCTATCATCATATATTTAATCAAGACGATATCCTTTAAAACTTATTC
AGTACTATAATCACTCTCGTTTCAAATTAATAAGATGTGCATGATTGCCCTAATATATGAAGAGGTATGA
TACAACCCTAACAGTGATCAAAGAAAATCATAATCTCGTATCGCTCGTAATATAACCTGCCAAGCATACC
TCTTGCACAAAGTGATTCTTGTACACAAATAATGTTTTACTCTACAGGAGGTAGCAACGATCCATCCCAT
CAAAAAATAAGTATTTCATGACTTACTAATGATCTCTTAAAATATTAAGAAAA

Protein Sequence : Show Sequence

>NP_066249.1 minor nucleoprotein [Zaire ebolavirus]
MEASYERGRPRAARQHSRDGHDHHVRARSSSRENYRGEYRQSRSASQVRVPTVFHKKRVEPLTVPPAPKD
ICPTLKKGFLCDSSFCKKDHQLESLTDRELLLLIARKTCGSVEQQLNITAPKDSRLANPTADDFQQEEGP
KITLLTLIKTAEHWARQDIRTIEDSKLRALLTLCAVMTRKFSKSQLSLLCETHLRREGLGQDQAEPVLEV
YQRLHSDKGGSFEAALWQQWDRQSLIMFITAFLNIALQLPCESSAVVVSGLRTLVPQSDNEEASTNPGTC
SWSDEGTP

Molecule Role : Protective antigen
Molecule Role Annotation : VP30 expressed from alphavirus replicons induced a protective immune response in BALB/c mice when challenged with Ebola virus Zaire (Wilson et al., 2001).
Additional Molecule Role : Protective antigen
Related Vaccine(s): VRP expressing VP30

16. VP35

Gene Name : VP35
Sequence Strain (Species/Organism) : Ebola virus
NCBI Protein GI : SCD11532
Other Database IDs : CDD:280298
Taxonomy ID : 1570291
Protein Name : VP35
Protein pI : 5.92
Protein Weight : 36093.95
Protein Length : 377
Protein Note : monopartite

Protein Sequence : Show Sequence

>SCD11532.1 VP35 [Ebola virus]
MTTRTKGRGHTVATTQNDRMPGPELSGWISEQLMTGRIPVNDIFCDIENNPGLCYASQMQQTKPNPKMRN
SQTQTDPICNHSFEEVVQTLASLATVVQQQTIASESLEQRITSLENGLKPVYDMAKTISSLNRVCAEMVA
KYDLLVMTTGRATATAAATEAYWAEHGQPPPGPSLYEESAIRGKIESRDETVPQSVREAFNNLDSTTSLT
EENFGKPDISAKDLRNIMYDHLPGFGTAFHQLVQVICKLGKDSNSLDIIHAEFQASLAEGDSPQCALIQI
TKRVPIFQDAAPPVIHIRSRGDIPRACQKSLRPVPPSPKIDRGWVCVFQLQDGKTLGLKI

Molecule Role : Protective antigen
Molecule Role Annotation :

17. VP35 from Reston ebolavirus

Gene Name : VP35 from Reston ebolavirus
Sequence Strain (Species/Organism) : Reston ebolavirus
VO ID : VO_0010863
NCBI Gene ID : 955189
NCBI Protein GI : 22789224
Locus Tag : REBOVgp2
Genbank Accession : AF522874
Protein Accession : NP_690581
Taxonomy ID : 186539
Gene Starting Position : 3018
Gene Ending Position : 4412
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 7.39
Protein Weight : 34622.88
Protein Length : 329

DNA Sequence : Show Sequence

>NC_004161.1:3018-4412 Reston ebolavirus isolate Reston virus/M.fascicularis-tc/USA/1989/Philippines89-Pennsylvania, complete genome
TGATGAAGATTAAAACCTTCATCGCCAGTAAATGATTATATTGTCTGTAGGCAGGTGTTTACTCCACCTT
AAATTTGGAAATATCCTACCTTAGGACCATTGTCAAGAGGTGCATAGGCATTACCACCCTTGAGAACATG
TACAATAATAAATTGAAGGTATGTTCAGGCCCAGAAACGACTGGATGGATTTCTGAGCAACTTATGACAG
GTAAGATTCCAGTAACTGATATATTCATTGATATTGATAACAAGCCAGATCAAATGGAAGTCCGACTCAA
ACCATCATCAAGGAGCTCAACAAGAACTTGTACAAGTAGCAGTCAGACGGAGGTCAACTATGTACCTCTC
CTTAAAAAGGTTGAGGATACATTAACTATGCTAGTGAATGCCACCAGTCGTCAGAATGCTGCAATCGAGG
CCCTTGAAAACCGCCTCAGCACACTTGAGAGTAGCTTAAAGCCAATCCAAGACATGGGTAAAGTGATTTC
ATCATTGAATCGCAGTTGTGCCGAAATGGTTGCAAAATATGATCTTCTAGTTATGACAACTGGACGGGCT
ACTTCAACTGCAGCTGCAGTAGATGCGTATTGGAAAGAGCACAAACAGCCACCACCAGGGCCAGCGTTGT
ATGAAGAGAATGCGCTTAAAGGAAAAATCGATGATCCAAACAGCTATGTACCAGATGCTGTGCAAGAGGC
TTACAAGAACCTTGACAGTACATCGACCCTGACCGAGGAAAATTTTGGGAAACCTTATATATCTGCTAAA
GACCTGAAGGAGATCATGTATGATCATCTACCTGGTTTTGGGACTGCCTTTCACCAACTTGTTCAAGTGA
TTTGTAAAATAGGAAAGGATAACAACCTTTTGGACACAATCCATGCTGAGTTCCAGGCAAGTCTAGCAGA
TGGTGACTCTCCCCAATGTGCACTCATACAGATAACCAAAAGGGTCCCAATCTTTCAGGATGTGCCGCCC
CCGATAATCCATATTAGATCCCGTGGTGACATCCCACGAGCATGCCAAAAGAGTCTCCGACCAGCACCAC
CATCACCCAAAATTGATCGTGGTTGGGTTTGTTTGTTTAAGATGCAAGATGGTAAAACGCTTGGACTTAA
GATCTAAGAATCAAGATTTATTTAACAAGGCAAGCCACAACCTTAGATGGAACCTCAGCCAGACTATTGA
ACTATTGACGCTGTTGATGATAATATATAATTAATGGTCTTATTTGAATATGACAACATCTTGCTTCTTG
TTCTGCCTTGTAGCTCTTTGAATTGGAAGATCATTCCAAACTTACAAACATGCACAAGATGTTATGGTTT
AGCAAAGAATTGATAGGAGTACTGGTATATAATGTAAATATAACAAGTGATGAAGATTAAGAAAA

Protein Sequence : Show Sequence

>NP_690581.1 polymerase complex protein [Reston ebolavirus]
MYNNKLKVCSGPETTGWISEQLMTGKIPVTDIFIDIDNKPDQMEVRLKPSSRSSTRTCTSSSQTEVNYVP
LLKKVEDTLTMLVNATSRQNAAIEALENRLSTLESSLKPIQDMGKVISSLNRSCAEMVAKYDLLVMTTGR
ATSTAAAVDAYWKEHKQPPPGPALYEENALKGKIDDPNSYVPDAVQEAYKNLDSTSTLTEENFGKPYISA
KDLKEIMYDHLPGFGTAFHQLVQVICKIGKDNNLLDTIHAEFQASLADGDSPQCALIQITKRVPIFQDVP
PPIIHIRSRGDIPRACQKSLRPAPPSPKIDRGWVCLFKMQDGKTLGLKI

Molecule Role : Protective antigen

18. VP35 from Zaire ebolavirus

Gene Name : VP35 from Zaire ebolavirus
Sequence Strain (Species/Organism) : Zaire ebolavirus
VO ID : VO_0010864
NCBI Gene ID : 911827
NCBI Protein GI : 10313992
Locus Tag : ZEBOVgp2
Genbank Accession : AF272001
Protein Accession : NP_066244
3D structure: PDB ID : 3FKE
Taxonomy ID : 186538
Gene Starting Position : 3031
Gene Ending Position : 4406
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 6.62
Protein Weight : 35575.45
Protein Length : 340

DNA Sequence : Show Sequence

>NC_002549.1:3031-4406 Zaire ebolavirus isolate Ebola virus/H.sapiens-tc/COD/1976/Yambuku-Mayinga, complete genome
TGATGAAGATTAAAACCTTCATCATCCTTACGTCAATTGAATTCTCTAGCACTCGAAGCTTATTGTCTTC
AATGTAAAAGAAAAGCTGGTCTAACAAGATGACAACTAGAACAAAGGGCAGGGGCCATACTGCGGCCACG
ACTCAAAACGACAGAATGCCAGGCCCTGAGCTTTCGGGCTGGATCTCTGAGCAGCTAATGACCGGAAGAA
TTCCTGTAAGCGACATCTTCTGTGATATTGAGAACAATCCAGGATTATGCTACGCATCCCAAATGCAACA
AACGAAGCCAAACCCGAAGACGCGCAACAGTCAAACCCAAACGGACCCAATTTGCAATCATAGTTTTGAG
GAGGTAGTACAAACATTGGCTTCATTGGCTACTGTTGTGCAACAACAAACCATCGCATCAGAATCATTAG
AACAACGCATTACGAGTCTTGAGAATGGTCTAAAGCCAGTTTATGATATGGCAAAAACAATCTCCTCATT
GAACAGGGTTTGTGCTGAGATGGTTGCAAAATATGATCTTCTGGTGATGACAACCGGTCGGGCAACAGCA
ACCGCTGCGGCAACTGAGGCTTATTGGGCCGAACATGGTCAACCACCACCTGGACCATCACTTTATGAAG
AAAGTGCGATTCGGGGTAAGATTGAATCTAGAGATGAGACCGTCCCTCAAAGTGTTAGGGAGGCATTCAA
CAATCTAAACAGTACCACTTCACTAACTGAGGAAAATTTTGGGAAACCTGACATTTCGGCAAAGGATTTG
AGAAACATTATGTATGATCACTTGCCTGGTTTTGGAACTGCTTTCCACCAATTAGTACAAGTGATTTGTA
AATTGGGAAAAGATAGCAACTCATTGGACATCATTCATGCTGAGTTCCAGGCCAGCCTGGCTGAAGGAGA
CTCTCCTCAATGTGCCCTAATTCAAATTACAAAAAGAGTTCCAATCTTCCAAGATGCTGCTCCACCTGTC
ATCCACATCCGCTCTCGAGGTGACATTCCCCGAGCTTGCCAGAAAAGCTTGCGTCCAGTCCCACCATCGC
CCAAGATTGATCGAGGTTGGGTATGTGTTTTTCAGCTTCAAGATGGTAAAACACTTGGACTCAAAATTTG
AGCCAATCTCCCTTCCCTCCGAAAGAGGCGAATAATAGCAGAGGCTTCAACTGCTGAACTATAGGGTACG
TTACATTAATGATACACTTGTGAGTATCAGCCCTGGATAATATAAGTCAATTAAACGACCAAGATAAAAT
TGTTCATATCTCGCTAGCAGCTTAAAATATAAATGTAATAGGAGCTATATCTCTGACAGTATTATAATCA
ATTGTTATTAAGTAACCCAAACCAAAAGTGATGAAGATTAAGAAAA

Protein Sequence : Show Sequence

>NP_066244.1 polymerase complex protein [Zaire ebolavirus]
MTTRTKGRGHTAATTQNDRMPGPELSGWISEQLMTGRIPVSDIFCDIENNPGLCYASQMQQTKPNPKTRN
SQTQTDPICNHSFEEVVQTLASLATVVQQQTIASESLEQRITSLENGLKPVYDMAKTISSLNRVCAEMVA
KYDLLVMTTGRATATAAATEAYWAEHGQPPPGPSLYEESAIRGKIESRDETVPQSVREAFNNLNSTTSLT
EENFGKPDISAKDLRNIMYDHLPGFGTAFHQLVQVICKLGKDSNSLDIIHAEFQASLAEGDSPQCALIQI
TKRVPIFQDAAPPVIHIRSRGDIPRACQKSLRPVPPSPKIDRGWVCVFQLQDGKTLGLKI

Molecule Role : Protective antigen
Molecule Role Annotation : VP35 expressed from alphavirus replicons induced a protective immune response in C57BL/6 mice when challenged with Ebola virus Zaire (Wilson et al., 2001).
Additional Molecule Role : Protective antigen
Related Vaccine(s): VRP expressing VP35

19. VP40 from Reston ebolavirus

Gene Name : VP40 from Reston ebolavirus
Sequence Strain (Species/Organism) : Reston ebolavirus
VO ID : VO_0010861
NCBI Gene ID : 955192
NCBI Protein GI : 22789225
Locus Tag : REBOVgp3
Genbank Accession : AF522874
Protein Accession : NP_690582
3D structure: PDB ID : 1ES6
Taxonomy ID : 186539
Gene Starting Position : 4395
Gene Ending Position : 5892
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 8.98
Protein Weight : 33340.06
Protein Length : 331

DNA Sequence : Show Sequence

>NC_004161.1:4395-5892 Reston ebolavirus isolate Reston virus/M.fascicularis-tc/USA/1989/Philippines89-Pennsylvania, complete genome
TGATGAAGATTAAGAAAAACCAGTCGGTATTTTCCAGACTTGGCATTTCTTATCTTCATCTTCTAAAGTG
AGATATTTTATCATCAAAAAATGAGGCGCGGAGTGTTACCAACGGCTCCTCCAGCATATAATGATATTGC
ATACCCTATGAGCATACTCCCAACCCGACCAAGTGTCATAGTCAATGAGACCAAATCAGATGTACTGGCA
GTGCCAGGGGCAGATGTTCCATCAAACTCCATGAGACCAGTGGCTGATGATAACATTGATCACTCAAGCC
ATACTCCAAGCGGAGTAGCTTCTGCCTTTATATTGGAAGCTACAGTGAATGTAATTTCGGGAACAAAAGT
CCTGATGAAGCAAATACCTATTTGGCTTCCACTGGGTGTAGCTGATCAGAAGATATACAGCTTTGATTCA
ACAACAGCCGCAATTATGTTGGCTTCCTACACAGTGACACACTTCGGGAAGATATCTAACCCGCTGGTAC
GTGTCAACAGGCTAGGCCCAGGAATACCCGATCATCCGCTACGACTCCTAAGGTTGGGCAATCAGGCATT
CCTTCAAGAGTTTGTTCTTCCACCAGTCCAGCTTCCCCAGTATTTCACATTTGATCTAACAGCTCTAAAG
CTCATCACTCAACCATTGCCAGCTGCAACCTGGACAGACGAAACTCCAGCAGGAGCAGTCAATGCTCTTC
GTCCTGGGCTCTCACTCCATCCCAAGCTTCGTCCAATTCTCCTGCCGGGGAAGACAGGAAAGAAAGGACA
TGCTTCAGACTTAACATCACCTGACAAGATTCAAACAATCATGAATGCAATACCGGACCTCAAAATTGTC
CCGATTGATCCAACCAAGAACATAGTTGGAATTGAGGTTCCAGAATTACTAGTTCAAAGGCTGACCGGCA
AAAAACCACAACCCAAAAATGGCCAACCAATTATTCCAGTTCTTCTTCCGAAATATGTTGGACTTGATCC
TATATCGCCAGGGGACTTAACTATGGTTATCACCCAGGATTGTGATTCATGCCACTCTCCAGCCAGCCAT
CCGTATCACATGGACAAGCAGAATAGTTACCAATAATTTAAATTCCATTCGAGCTATTATTCTGCTAGTA
ATTCCGACGGGATCAATAGACTAAAAATCTGATTGTATAGAATTATAAAAGAATCAAGCAGAGGCAACAG
ACTCACAGCTTACGCCTAGATAACTAATATTAAGGAGTTTTTTAATCTAATTTTCCAGTCTTGAGTAATA
ATCATTTCTTTTGTAATTAATTATGCATTTGTTAACTTATCGGTGCGAGATTTCCTTGAGAACCCGGCGG
AGCTTCTACTATCTGCAGTAACCAGAAGAGAAGTTCAACCCAGTCAAAACTAAACCAAGCAATATTCTGA
ATGCTCTATAGTCTATTCTAATCAGAGGTATAACAATGGCTAAGATTTCAATGACTCGTTAACAATCGCT
AGTAATTTTAATCTCCAGATTAAGAAAA

Protein Sequence : Show Sequence

>NP_690582.1 matrix protein [Reston ebolavirus]
MRRGVLPTAPPAYNDIAYPMSILPTRPSVIVNETKSDVLAVPGADVPSNSMRPVADDNIDHSSHTPSGVA
SAFILEATVNVISGTKVLMKQIPIWLPLGVADQKIYSFDSTTAAIMLASYTVTHFGKISNPLVRVNRLGP
GIPDHPLRLLRLGNQAFLQEFVLPPVQLPQYFTFDLTALKLITQPLPAATWTDETPAGAVNALRPGLSLH
PKLRPILLPGKTGKKGHASDLTSPDKIQTIMNAIPDLKIVPIDPTKNIVGIEVPELLVQRLTGKKPQPKN
GQPIIPVLLPKYVGLDPISPGDLTMVITQDCDSCHSPASHPYHMDKQNSYQ

Molecule Role : Protective antigen

20. VP40 from Zaire ebolavirus

Gene Name : VP40 from Zaire ebolavirus
Sequence Strain (Species/Organism) : Zaire ebolavirus
VO ID : VO_0010868
NCBI Gene ID : 911825
NCBI Protein GI : 10313993
Locus Tag : ZEBOVgp3
Genbank Accession : AF272001
Protein Accession : NP_066245
3D structure: PDB ID : 1ES6
Taxonomy ID : 186538
Gene Starting Position : 4389
Gene Ending Position : 5893
Gene Strand (Orientation) : +
Protein Name : mRNA
Protein pI : 9.02
Protein Weight : 33197.88
Protein Length : 326

DNA Sequence : Show Sequence

>NC_002549.1:4389-5893 Zaire ebolavirus isolate Ebola virus/H.sapiens-tc/COD/1976/Yambuku-Mayinga, complete genome
TGATGAAGATTAAGAAAAACCTACCTCGGCTGAGAGAGTGTTTTTTCATTAACCTTCATCTTGTAAACGT
TGAGCAAAATTGTTAAAAATATGAGGCGGGTTATATTGCCTACTGCTCCTCCTGAATATATGGAGGCCAT
ATACCCTGTCAGGTCAAATTCAACAATTGCTAGAGGTGGCAACAGCAATACAGGCTTCCTGACACCGGAG
TCAGTCAATGGGGACACTCCATCGAATCCACTCAGGCCAATTGCCGATGACACCATCGACCATGCCAGCC
ACACACCAGGCAGTGTGTCATCAGCATTCATCCTTGAAGCTATGGTGAATGTCATATCGGGCCCCAAAGT
GCTAATGAAGCAAATTCCAATTTGGCTTCCTCTAGGTGTCGCTGATCAAAAGACCTACAGCTTTGACTCA
ACTACGGCCGCCATCATGCTTGCTTCATACACTATCACCCATTTCGGCAAGGCAACCAATCCACTTGTCA
GAGTCAATCGGCTGGGTCCTGGAATCCCGGATCATCCCCTCAGGCTCCTGCGAATTGGAAACCAGGCTTT
CCTCCAGGAGTTCGTTCTTCCGCCAGTCCAACTACCCCAGTATTTCACCTTTGATTTGACAGCACTCAAA
CTGATCACCCAACCACTGCCTGCTGCAACATGGACCGATGACACTCCAACAGGATCAAATGGAGCGTTGC
GTCCAGGAATTTCATTTCATCCAAAACTTCGCCCCATTCTTTTACCCAACAAAAGTGGGAAGAAGGGGAA
CAGTGCCGATCTAACATCTCCGGAGAAAATCCAAGCAATAATGACTTCACTCCAGGACTTTAAGATCGTT
CCAATTGATCCAACCAAAAATATCATGGGAATCGAAGTGCCAGAAACTCTGGTCCACAAGCTGACCGGTA
AGAAGGTGACTTCTAAAAATGGACAACCAATCATCCCTGTTCTTTTGCCAAAGTACATTGGGTTGGACCC
GGTGGCTCCAGGAGACCTCACCATGGTAATCACACAGGATTGTGACACGTGTCATTCTCCTGCAAGTCTT
CCAGCTGTGATTGAGAAGTAATTGCAATAATTGACTCAGATCCAGTTTTATAGAATCTTCTCAGGGATAG
TGATAACATCTATTTAGTAATCCGTCCATTAGAGGAGACACTTTTAATTGATCAATATACTAAAGGTGCT
TTACACCATTGTCTTTTTTCTCTCCTAAATGTAGAACTTAACAAAAGACTCATAATATACTTGTTTTTAA
AGGATTGATTGATGAAAGATCATAACTAATAACATTACAAATAATCCTACTATAATCAATACGGTGATTC
AAATGTTAATCTTTCTCATTGCACATACTTTTTGCCCTTATCCTCAAATTGCCTGCATGCTTACATCTGA
GGATAGCCAGTGTGACTTGGATTGGAAATGTGGAGAAAAAATCGGGACCCATTTCTAGGTTGTTCACAAT
CCAAGTACAGACATTGCCCTTCTAATTAAGAAAAA

Protein Sequence : Show Sequence

>NP_066245.1 matrix protein [Zaire ebolavirus]
MRRVILPTAPPEYMEAIYPVRSNSTIARGGNSNTGFLTPESVNGDTPSNPLRPIADDTIDHASHTPGSVS
SAFILEAMVNVISGPKVLMKQIPIWLPLGVADQKTYSFDSTTAAIMLASYTITHFGKATNPLVRVNRLGP
GIPDHPLRLLRIGNQAFLQEFVLPPVQLPQYFTFDLTALKLITQPLPAATWTDDTPTGSNGALRPGISFH
PKLRPILLPNKSGKKGNSADLTSPEKIQAIMTSLQDFKIVPIDPTKNIMGIEVPETLVHKLTGKKVTSKN
GQPIIPVLLPKYIGLDPVAPGDLTMVITQDCDTCHSPASLPAVIEK

Molecule Role : Protective antigen
Molecule Role Annotation : Vaccination of BALB/c or C57BL/6 mice with eVLPs, composed of the EBOV glycoprotein and matrix viral protein (VP)40 with a lipid membrane, in conjunction with QS-21 adjuvant resulted in mixed IgG subclass responses, a Th1-like memory cytokine response, and protection from lethal EBOV challenge (Warfield et al., 2005).

VP40 expressed from alphavirus replicons induced a protective immune response in BALB/c mice when challenged with Ebola virus Zaire (Wilson et al., 2001).
Related Vaccine(s): rVSV- SEBOV-GP and -VP40 , VRP expressing VP40

21. ZGP

Gene Name : ZGP
Sequence Strain (Species/Organism) : Zaire ebolavirus strain Zaire95
NCBI Nucleotide GI : 1695251
NCBI Protein GI : 1695253
Protein Accession : AAB37095.1
Other Database IDs : CDD:279888
CDD:333284
CDD:197367
Taxonomy ID : 186538
Gene Strand (Orientation) : ?
Protein Name : virion spike glycoprotein
Protein pI : 6.69
Protein Weight : 71315.12
Protein Length : 744
Protein Note : Filovirus glycoprotein; pfam01611

DNA Sequence : Show Sequence

>gi|1695251|gb|U28077.1|EVU28077 Zaire Ebola virus strain Zaire95 virion spike glycoprotein (SP) gene, complete cds, and small/secreted glycoprotein precursor (SGP) gene, complete cds
GCGATGAAGATTAAGCCGACAGTGAGCGTAATCTTCATCTCTCTTAGATTATTTGTCCTCCAGAGTAGGG
ATCGTCAGGTCCTTTTCAATCGTATAACCAAAATAAACTTCACTAGAAGGATATTGTGGGGCAACAACAC
AATGGGTGTTACAGGAATATTGCAGTTACCTCGTGATCGATTCAAGAGGACATCATTCTTTCTTTGGGTA
ATTATCCTTTTCCAAAGAACATTTTCCATCCCACTTGGAGTCATCCACAATAGCACATTACAGGTTAGTG
ATGTCGACAAACTGGTTTGCCGTGACAAACTGTCATCCACAAATCAATTGAGATCAGTTGGACTGAATCT
CGAAGGGAATGGAGTGGCAACTGACGTGCCATCTGCAACTAAAAGATGGGGCTTCAGGTCCGGTGTCCCA
CCAAAGGTGGTCAATTATGAAGCTGGTGAATGGGCTGAAAACTGCTACAATCTTGAAATCAAAAAACCTG
ACGGGAGTGAGTGTCTACCAGCAGCGCCAGACGGGATTCGGGGCTTCCCCCGGTGCCGGTATGTGCACAA
AGTATCAGGAACGGGACCGTGTGCCGGAGACTTTGCCTTCCACAAAGAGGGTGCTTTCTTCCTGTATGAC
CGACTTGCTTCCACAGTTATCTACCGAGGAACGACTTTCGCTGAAGGTGTCGTTGCATTTCTGATACTGC
CCCAAGCTAAGAAGGACTTCTTCAGCTCACACCCCTTGAGAGAGCCGGTCAATGCAACGGAGGACCCGTC
TAGTGGCTACTATTCTACCACAATTAGATATCAAGCTACCGGTTTTGGAACCAATGAGACAGAGTATTTG
TTCGAGGTTGACAATTTGACCTACGTCCAACTTGAATCAAGATTCACACCACAGTTTCTGCTCCAGCTGA
ATGAGACAATATATACAAGTGGGAAAAGGAGCAATACCACGGGAAAACTAATTTGGAAGGTCAACCCCGA
AATTGATACAACAATCGGGGAGTGGGCCTTCTGGGAAACTAAAAAAACCTCACTAGAAAAATTCGCAGTG
AAGAGTTGTCTTTCACAGCTGTATCAAACAGAGCCAAAAACATCAGTGGTCAGAGTCCGGCGCGAACTTC
TTCCGACCCAGGGACCAACACAACAACTGAAGACCACAAAATCATGGCTTCAGAAAATTCCTCTGCAATG
GTTCAAGTGCACAGTCAAGGAAGGGAAGCTGCAGTGTCGCATCTGACAACCCTTGCCACAATCTCCACGA
GTCCTCAACCCCCCACAACCAAACCAGGTCCGGACAACAGCACCCACAATACACCCGTGTATAAACTTGA
CATCTCTGAGGCAACTCAAGTTGAACAACATCACCGCAGAACAGACAACGACAGCACAGCCTCCGACACT
CCCCCCGCCACGACCGCAGCCGGACCCCTAAAAGCAGAGAACACCAACACGAGCAAGGGTACCGACCTCC
TGGACCCCGCCACCACAACAAGTCCCCAAAACCACAGCGAGACCGCTGGCAACAACAACACTCATCACCA
AGATACCGGAGAAGAGAGTGCCAGCAGCGGGAAGCTAGGCTTAATTACCAATACTATTGCTGGAGTCGCA
GGACTGATCACAGGCGGGAGGAGAGCTCGAAGAGAAGCAATTGTCAATGCTCAACCCAAATGCAACCCTA
ATTTACATTACTGGACTACTCAGGATGAAGGTGCTGCAATCGGACTGGCCTGGATACCATATTTCGGGCC
AGCAGCCGAGGGAATTTACACAGAGGGGCTGATGCACAATCAAGATGGTTTAATCTGTGGGTTGAGACAG
CTGGCCAACGAGACGACTCAAGCTCTTCAACTGTTCCTGAGAGCCACAACCGAGCTACGCACCTTTTCAA
TCCTCAACCGTAAGGCAATTGATTTCTTGCTGCAGCGATGGGGCGGCACATGCCACATTTTGGGACCGGA
CTGCTGTATCGAACCACATGATTGGACCAAGAACATAACAGACAAAATTGATCAGATTATTCATGATTTT
GTTGATAAAACCCTTCCGGACCAGGGGGACAATGACAATTGGTGGACAGGATGGAGACAATGGATACCGG
CAGGTATTGGAGTTACAGGCGTTATAATTGCAGTTATCGCTTTATTCTGTATATGCAAATTTGTCTTTTA
GTTTTTCTTCAGATTGCTTCATGGCAAAGCTCAGCCTCAAATCAATGAAACCAGGATTTAATTATATGGA
TTACTTGAATCTAAGATTACTTGACAAATGATAATATAATACACTGGAGCTTTAAACATAGCCAATGTGA
TTCTAACTCTTTTAAACTCACAGTTAATCATAAATAAGGTTTGACATCAATCTAGTTATCTCTTTGAGAA
TGATAAACTTGATGAAGATTAAGAAAAA

Protein Sequence : Show Sequence

>AAB37095.1 virion spike glycoprotein [Zaire ebolavirus]
MGVTGILQLPRDRFKRTSFFLWVIILFQRTFSIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNL
EGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHK
VSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPS
SGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPE
IDTTIGEWAFWETKKNLTRKIRSEELSFTAVSNRAKNISGQSPARTSSDPGTNTTTEDHKIMASENSSAM
VQVHSQGREAAVSHLTTLATISTSPQPPTTKPGPDNSTHNTPVYKLDISEATQVEQHHRRTDNDSTASDT
PPATTAAGPLKAENTNTSKGTDLLDPATTTSPQNHSETAGNNNTHHQDTGEESASSGKLGLITNTIAGVA
GLITGGRRARREAIVNAQPKCNPNLHYWTTQDEGAAIGLAWIPYFGPAAEGIYTEGLMHNQDGLICGLRQ
LANETTQALQLFLRATTELRTFSILNRKAIDFLLQRWGGTCHILGPDCCIEPHDWTKNITDKIDQIIHDF
VDKTLPDQGDNDNWWTGWRQWIPAGIGVTGVIIAVIALFCICKFVF

Molecule Role : Protective antigen
Related Vaccine(s): Ebola Virus Vaccine Ad5-ZGP

III. Vaccine Information

1. cAd3-EBO S

a. Product Name:

Ebola Sudan Chimpanzee Adenovirus Vector Vaccine

b. Type:

Recombinant vector vaccine

c. Status:

Licensed

d. Location Licensed:

Kampala, Uganda

e. Host Species for Licensed Use:

Human

f. Gene Engineering of GP from Sudan ebolavirus

Type: Recombinant vector construction
Description: The recombinant chimpanzee adenovirus Type 3-vectored Ebola vaccine, VRC-EBOADC086-00-VP (cAd3-EBO S), is composed of a cAd3 vector that encodes Ebola Sudan wild type glycoprotein (WT GP).(Ledgerwood et al., 2017)
Detailed Gene Information: Click here.

g. Gene Engineering of GP from Zaire ebolavirus

Type: Recombinant vector construction
Description: cAd3-EBO glycoprotein Zaire and cAd3-EBO glycoprotein Sudan were made and mixed in a 1:1 ratio (Ledgerwood et al., 2017)
Detailed Gene Information: Click here.

h. Vector:

Recombinant vector chimpanzee (Ledgerwood et al., 2017)

i. Immunization Route

Intramuscular injection (i.m.)

2. cAdVax-based bivalent ebola virus vaccine (Sudan and Zaire species)

a. Vaccine Ontology ID:

VO_0004647

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Human

e. Gene Engineering of GP from Sudan ebolavirus

Type: Recombinant protein preparation
Description: The gene was inserted into a single complex adenovirus-based vaccine (cAdVax) vector (Wang et al., 2006).
Detailed Gene Information: Click here.

f. Gene Engineering of GP from Zaire ebolavirus

Type: Recombinant protein preparation
Description: The gene was inserted into a single complex adenovirus-based vaccine (cAdVax) vector (Wang et al., 2006).
Detailed Gene Information: Click here.

g. Preparation

The bivalent cAdVaxE(GPs/z) vaccine includes the SEBOV glycoprotein (GP) and ZEBOV GP genes together in a single complex adenovirus-based vaccine (cAdVax) vector (Wang et al., 2006).

h. Immunization Route

Intramuscular injection (i.m.)

i. Mouse Response

Vaccine Immune Response Type: VO_0003057
Immune Response: Vaccination of mice with the bivalent cAdVaxE(GPs/z) vaccine led to efficient induction of EBOV-specific antibody and cell-mediated immune responses to both species of EBOV (Wang et al., 2006).
Challenge Protocol: Mice were challenged with a lethal dose of ZEBOV (30,000 times the 50% lethal dose) (Wang et al., 2006).
Efficacy: the cAdVax technology demonstrated induction of a 100% protective immune response in mice, as all vaccinated C57BL/6 and BALB/c mice survived challenge with a lethal dose of ZEBOV (Wang et al., 2006).

3. CAdVax-Filoviruses (Ebola )

a. Vaccine Ontology ID:

VO_0004644

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Preparation

A panfilovirus vaccine based on a complex adenovirus (CAdVax) technology that expresses multiple antigens from five different filoviruses de novo (Swenson et al., 2008).

f. Immunization Route

Intramuscular injection (i.m.)

g. Macaque Response

Vaccination Protocol: The macaques in the vaccine groups (five per group) were anesthetized by intramuscular injection of ketamine HCl (10 mg/kg of body weight), followed by intramuscular vaccination with an equal mixture of 1 × 10^10 PFU of each vaccine component: EBO2, EBO7, M8, and M11 (resulting in 4 × 10^10 total PFU per animal). Control animals received 4 × 10^10 PFU of the HC4 vaccine vector, also via the intramuscular route (Swenson et al., 2008).
Vaccine Immune Response Type: VO_0000287
Challenge Protocol: Group 1 was inoculated subcutaneously with MARV Musoke, while group 2 was inoculated intramuscularly with ZEBOV, using approximately 1,000 PFU of each filovirus. EBOV and MARV each have different established routes of administration (intramuscular and subcutaneous, respectively) (Swenson et al., 2008).
Efficacy: All vaccinated animals showed no detectable viremia or hematology abnormalities. We can conclude that the multivalent filovirus vaccine was 100% protective against lethal MARV Musoke and ZEBOV challenges (Swenson et al., 2008).

4. CAdVax-ZEBOV/SEBOV

a. Vaccine Ontology ID:

VO_0004641

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Preparation

A multivalent vaccine candidate (EBO7) that expresses the glycoproteins of Zaire ebolavirus (ZEBOV) and Sudan ebolavirus (SEBOV) in a single complex adenovirus-based vector (CAdVax) (Pratt et al., 2010).

f. Immunization Route

Intramuscular injection (i.m.)

g. Macaque Response

Vaccination Protocol: For the parenteral challenge studies, cynomolgus macaques were vaccinated intramuscularly (i.m.) on day zero with a 1:1 mixture of 1 × 10^10 PFU each of EBO7 and M8 (total 2 × 10^10 PFU). Control animals received an i.m. injection of 2 × 10^10 PFU of HC4. For the initial aerosol infection experiments, cynomolgus macaques were vaccinated by i.m. injection of 1 × 10^10 PFU of EBO7 or 1 × 10^10 PFU of HC4 (Pratt et al., 2010).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: For the aerosol infection group, twenty-eight days after vaccination, animals were anesthetized and exposed to a target dose of 1,000 PFU of either aerosolized ZEBOV or aerosolized SEBOV. For the parenteral challenge studies, six weeks after the boosting vaccinations, the macaques were anesthetized by i.m. injection of Telazol (2 to 6 mg/kg of body weight) and then inoculated i.m. with SEBOV or ZEBOV challenge stock (Pratt et al., 2010).
Efficacy: EBO7 vaccine provided protection against both Ebola viruses by either route of infection. Significantly, protection against SEBOV given as an aerosol challenge, which has not previously been shown, could be achieved with a boosting vaccination (Pratt et al., 2010).

5. DNA vaccine expressing sGP

a. Vaccine Ontology ID:

VO_0000785

b. Type:

DNA vaccine

c. Gene Engineering of GP from Reston ebolavirus

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

d. Vector:

pCMV (Xu et al., 1998)

e. Preparation

Plasmids containing the sGP cDNAs are used to subclone the relevant inserts into CMV expression vectors, which utilize the bovine growth hormone polyadenylation sequence. The plasmid pCRII-sGP is digested with EcoRI and treated with Klenow enzyme, and the resulting fragment is inserted into the BamHI/Bg/II CMV plasmid, which has been incubated with Klenow fragment and calf intestinal phosphate (CIP), and phenol chloroform extracted (Xu et al., 1998).

f. Description

sGP is a secreted or transmembrane form of glycoprotein (Xu et al., 1998).

g. Guinea pig Response

Vaccination Protocol: Two groups of guinea pigs were immunized by injection of 0.5 mg/ml in each hind leg (two injections at each time point) with the plasmed expression vectors. Animals were challenged by inoculation with a stock of Ebola virus that had been passaged once in Vero E6 cells and serially passaged by intraperitoneal injection of slpeen homogenates in Hartley guinea pigs seven times. Immunized guinea pigs were injected intraperitoneally with 0.5 ml of a 1:1000 dillution of spleen cell homogenate in Hanks' balanced salt solution 122 days after the initial plasmid DNA injection. Survival was determined 10 days later at which times animals were killed for serologic and pathologic analysis (Xu et al., 1998).
Persistence: None noted
Immune Response: A broad immune response was conferred by sGP which induced both cellular and humoral immunity to the membrane-associated GP. The ability of vectors expressing GP to confer immunity may be explained by the generation of the lower molecular weight degradation products, which could provide sufficient protein for antigen presentation to induce detectable, cellular and humoral immune responses in guinea pigs (Xu et al., 1998).
Side Effects: None noted
Efficacy: For the first group of 6 giunea pigs, animals were challenged within 2 months after the initial immunization. Five of six of the immunized subjects survived in contrast to 0/6 control subjects. In the second group, guinea pigs were challenged 4 months after the initial immunization. Three of the five guinea pigs immunized with sGP showed no ill effects following the viral challenge (Xu et al., 1998).

6. Ebola virus DNA vaccine DNA/rAd5 encoding ZEBOV and SEBOV antigens

a. Vaccine Ontology ID:

VO_0004385

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Macaque

e. Gene Engineering of GP from Sudan ebolavirus

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

f. Gene Engineering of GP from Zaire ebolavirus

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

g. Vector:

Replication-defective rAd5 GP vectors and p1012 (Hensley et al., 2010)

h. Immunization Route

Intramuscular injection (i.m.)

i. Macaque Response

Vaccination Protocol: Four cynomolgus macaques were injected at 4–6 week intervals with GP(Z) and GP(S/G) DNA, followed by a rest period, and boosted after one year with rAd5 vectors containing the EBOV matched insert (Hensley et al., 2010).
Vaccine Immune Response Type: VO_0000286
Immune Response: DNA/rAd prime-boost EBOV immunization generated antigen-specific CD4+ T cell immunity against proteins expressed by the vaccine insert. The magnitude of antigen-specific CD4+ T cells was uniform across the four immunized macaques (Hensley et al., 2010).
Efficacy: DNA/rAd5 immunization of cynomolgus macaques protects against infection when animals are challenged with a virus species homologous to the vaccine inserts (Hensley et al., 2010).

7. Ebola virus DNA vaccine EBOV GP

a. Vaccine Ontology ID:

VO_0004036

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse, rabbit, guinea pig

e. Gene Engineering of GP from Zaire ebolavirus

Type: DNA vaccine construction
Description: Vector pWRG7077 expressed Ebola virus GP gene sequences (Riemenschneider et al., 2003).
Detailed Gene Information: Click here.

f. Vector:

pWRG7077 (Riemenschneider et al., 2003)

g. Immunization Route

Gene gun

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Immune Response: The mice had low neutralizing antibody responses, yet were protected from challenge. This, as indicated by earlier studies, may be due to gene gun vaccination, which generally elicits a TH2-type response in BALB/c mice (Riemenschneider et al., 2003).
Efficacy: Two vaccinations with the EBOV GP DNA elicited consistently high antibody responses and conferred complete protection from EBOV challenge (Riemenschneider et al., 2003).

8. Ebola virus DNA vaccine encoding ZEBOV GP and SEBOV GP

a. Vaccine Ontology ID:

VO_0011465

b. Type:

DNA vaccine

c. Status:

Research

d. Antigen

Zaire Ebola virus GP and Sudan Ebola virus GP

e. Gene Engineering of GP from Zaire ebolavirus

Type: DNA vaccine construction
Description: DNA/rAd5 vaccines expressing ZEBOV and SEBOV glycoprotein (GP) (Hensley et al., 2010).
Detailed Gene Information: Click here.

f. Gene Engineering of GP from Sudan ebolavirus

Type: DNA vaccine construction
Description: DNA/rAd5 vaccines expressing ZEBOV and SEBOV glycoprotein (GP) (Hensley et al., 2010).
Detailed Gene Information: Click here.

g. Vector:

Replication-defective rAd5 GP vectors and p1012 (Hensley et al., 2010)

h. Immunization Route

Intramuscular injection (i.m.)

i. Macaque Response

Vaccination Protocol: DNA immunizations were administered by Biojector IM injection, bilateral deltoid, with a mixture of 2 mg each of two plasmid vectors encoding GP(Z) and GP(S/G). DNA immunizations were administered at 0, 4, 8, and 14 weeks. Each subject received a boost with 10^11 particle units (PU) of rAd5 GP(Z) at 12 months following the final DNA priming immunization (Hensley et al., 2010).
Challenge Protocol: All animals were challenged by the intramuscular route with 1,000 TCID50 of BEBOV, 7 weeks post rAd5 GP boost. The challenge virus used in this study was isolated from blood specimen #200706291 from a fatal case infected during the 2007 EBOV outbreak in Bundibugyo district, Uganda. The virus was isolated on Vero E6 cells and passaged twice prior to initiating these experiments (Hensley et al., 2010).
Efficacy: Vaccinated subjects developed robust, antigen-specific humoral and cellular immune responses against the GP from ZEBOV as well as cellular immunity against BEBOV GP, and immunized macaques were uniformly protected against lethal challenge with BEBOV (Hensley et al., 2010).

9. Ebola virus DNA vaccine GP DNA

a. Vaccine Ontology ID:

VO_0004334

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of GP from Zaire ebolavirus

Type: DNA vaccine construction
Description: Vector pWRG7077 expressed highly glycosylated type 1 transmembrane protein (GP) (Vanderzanden et al., 1998).
Detailed Gene Information: Click here.

f. Vector:

pWRG7077 (Vanderzanden et al., 1998)

g. Immunization Route

PowderJect-XR gene gun

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Efficacy: Mice were completely protected from challenge with mouse adapted EBOV with a priming dose of 0.5 microgram of GP DNA followed by three or four subsequent vaccinations with 1.5 micrograms of DNA. Partial protection could be observed for at least 9 months after three immunizations with 0.5 microgram of the GP DNA vaccine (Vanderzanden et al., 1998).

10. Ebola virus EBOV NP

a. Vaccine Ontology ID:

VO_0004037

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Rabbit, mouse, guinea pig

e. Gene Engineering of NP from Zaire Ebola virus

Type: DNA vaccine construction
Description: Vector pWRG7077 expressed Ebola virus NP gene sequences (Riemenschneider et al., 2003).
Detailed Gene Information: Click here.

f. Vector:

pWRG7077 (Riemenschneider et al., 2003)

g. Immunization Route

Gene gun

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Immune Response: The mice had low neutralizing antibody responses, yet were protected from challenge. This, as indicated by earlier studies, may be due to gene gun vaccination, which generally elicits a TH2-type response in BALB/c mice (Riemenschneider et al., 2003).
Efficacy: Two vaccinations with the EBOV NP DNA elicited consistently high antibody responses and conferred complete protection from EBOV challenge (Riemenschneider et al., 2003).

11. Ebola virus recombinant adenovirus vaccine AdC7-ZGP encoding GP

a. Vaccine Ontology ID:

VO_0004382

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of GP from Zaire ebolavirus

Type: Recombinant vector construction
Description: Vector adenoviral (ADV) expressed the Ebola envelope glycoprotein (GP) (Kobinger et al., 2006).
Detailed Gene Information: Click here.

f. Vector:

adenoviral (ADV) vector (Kobinger et al., 2006)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Immune Response: AdC7 vaccine stimulated robust T and B cell responses to ZEBOV in naïve mice (Kobinger et al., 2006).
Efficacy: Mice were immunized with a single dose of 5 × 10^10 particles per animal as performed previously and vaccinated animals were challenged with 200 LD50 of the mouse-adapted strain of ZEBOV 21 days later. All control mice (vehicle and AdHu5-LacZ) died between days 5 and 9 post-challenge. In contrast, all mice vaccinated with AdC7-ZGP survived the challenge with mouse-adapted ZEBOV. Weight loss was observed only from control groups (vehicle and AdHu5-LacZ). Complete protection following vaccination with 5 × 10^10 particles of AdC7-ZGP was demonstrated with challenge doses of ZEBOV up to 200,000 LD50, which was the highest dose tested (Kobinger et al., 2006).

12. Ebola virus recombinant adenovirus vector vaccine ADV−GP/NP

a. Vaccine Ontology ID:

VO_0004072

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Gene Engineering of GP from Zaire ebolavirus

Type: DNA vaccine construction
Description: Vector adenoviral (ADV) expressed Ebola glycoprotein (GP) (Sullivan et al., 2003b).
Detailed Gene Information: Click here.

e. Gene Engineering of NP from Zaire Ebola virus

Type: Recombinant vector construction
Description: Vector adenoviral (ADV) expressed Ebola nucleoprotein (NP) (Sullivan et al., 2003b).
Detailed Gene Information: Click here.

f. Preparation

To make ADV-GP, the BamHI/EcoRI fragment of GP(Z) was digested from PGEM-3Zf(-)-GP, treated with Klenow, and inserted into HindIII/XbaI/Kle/CIP-treated pRc/CMV plasmid. The resulting plasmid was digested by NruI/DraIII and treated with Klenow. The NruI/DraIII/Kle fragment containing the CMV enhancer, GP(Z) DNA and bovine growth hormone polyadenylation signal was inserted into the BgIII site of the adenoviral shuttle plasmid pAdBgIII26. The adenovirus, a first generation dl 309-based Ad5 vector, contained a deletion in E1 to render the vector replication defective and a partial deletion/substitution in E3, which disrupts the coding sequences for the E3 proteins with a relative molecular mass of 14,700, 14,500 and 10,400, respectively (Sullivan et al., 2000).

g. Virulence

h. Description

ADV−GP consists of an adenoviral (ADV) vector encoding the Ebola glycoprotein (GP) (Sullivan et al., 2003).

i. Monkey Response

Host Strain: Cynomolgus macaque
Vaccination Protocol: Cynomolgus macaques were immunized with ADV−GP and ADV−NP, followed by boosting 9 weeks later (Sullivan et al., 2003).
Persistence: None noted
Side Effects: None noted
Challenge Protocol: One week after the boost, animals were challenged with either a low or high dose of a 1995 isolate of Ebola virus Zaire.
Efficacy: In the saline-injected control animals these doses were uniformly fatal 6−12 days afterwards. In contrast, the ADV−GP/NP immunized monkeys were completely protected. Analysis of the cell-mediated and humoral immune responses revealed significant increases in the CD8+ T-cell response to Ebola antigens by intracellular cytokine staining for interferon (IFN)-, seen before exposure to virus, in contrast to control animals where no response was seen. Similarly, antibody titres to the virus were stimulated in vaccinated animals, which minimally increased after the viral challenge (Sullivan et al., 2003).

13. Ebola virus recombinant rAD-GP encoding GP

a. Vaccine Ontology ID:

VO_0004335

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Macaque

e. Gene Engineering of GP from Zaire ebolavirus

Type: Recombinant vector construction
Description: Vector rAd expressed the Ebola glycoprotein (GP) (Sullivan et al., 2006).
Detailed Gene Information: Click here.

f. Vector:

replication-defective adenoviral vectors (rAd) (Sullivan et al., 2006)

g. Immunization Route

Intramuscular injection (i.m.)

h. Macaque Response

Vaccine Immune Response Type: VO_0000286
Efficacy: Expression of specific GPs alone vectored by rAd are sufficient to confer protection against lethal challenge in a relevant nonhuman primate model (Sullivan et al., 2006).

14. Ebola virus recombinant vector vaccine Ad-CAGoptZGP encoding the envelope glycoprotein

a. Vaccine Ontology ID:

VO_0004384

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse, guinea pig

e. Gene Engineering of GP from Zaire ebolavirus

Type: Recombinant vector construction
Description: Vector Human adenovirus serotype 5 (Ad) expressed the Zaire ebolavirus (ZEBOV) envelope glycoprotein (Richardson et al., 2009).
Detailed Gene Information: Click here.

f. Vector:

Human adenovirus serotype 5 (Ad) (Richardson et al., 2009)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Immune Response: Ad-CAGoptZGP at a dose of 1×10^5 IFU/mouse elicited a frequency of 1.3±0.3% positive IFN-γ producing CD8+ T cells. Overall, the average frequency of positive IFN-γ producing CD8+ T cells was slightly higher with a lower dose of Ad-CAGoptZGP than with a higher dose of Ad-CMVZGP (Richardson et al., 2009).
Efficacy: All mice vaccinated with doses of 1×10^4, 1×10^5 and 1×10^6 IFU/mouse of Ad-CAGoptZGP were fully protected from the viral challenge with no weight loss or other clinical signs of disease (Richardson et al., 2009).

15. Ebola virus recombinant vector vaccine Ad-CMVZGP encoding the glycoprotein

a. Vaccine Ontology ID:

VO_0004383

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of GP from Zaire ebolavirus

Type: Recombinant vector construction
Description: Vector Human adenovirus serotype 5 (Ad) expressed the Zaire ebolavirus (ZEBOV) envelope glycoprotein (Richardson et al., 2009).
Detailed Gene Information: Click here.

f. Vector:

Human adenovirus serotype 5 (Ad) (Richardson et al., 2009)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Immune Response: Vaccination following viral challenge produced enhanced T and B cell immune responses with a low dose of Ad-CAGoptZGP. The frequency of IFN-γ+CD8 T cells with Ad-CMVZGP at 1×10^7 IFU/mouse was at 1.6±0.4%, on average (Richardson et al., 2009).
Efficacy: Protection following Zaire ebola virus challenge was complete in mice vaccinated with Ad-CMVZGP at 1×10^6 and 1×10^7 IFU/mouse but was only partially protective at 1×10^5 IFU/mouse (Richardson et al., 2009).

16. Ebola virus recombinant vector vaccine EBO7 encoding GP from SEBOV and ZEBOV

a. Vaccine Ontology ID:

VO_0004337

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Macaque

e. Gene Engineering of GP from Sudan ebolavirus

Type: Recombinant vector construction
Description: Vector CAdVax expressed GP from SEBOV (Pratt et al., 2010).
Detailed Gene Information: Click here.

f. Gene Engineering of GP from Zaire ebolavirus

Type: Recombinant vector construction
Description: Vector CAdVax expressed GP from ZEBOV (Pratt et al., 2010).
Detailed Gene Information: Click here.

g. Vector:

a bivalent rAd vector (CAdVax) (Pratt et al., 2010)

h. Immunization Route

Intramuscular injection (i.m.)

i. Macaque Response

Vaccine Immune Response Type: VO_0000286
Efficacy: Significantly, protection against SEBOV given as an aerosol challenge, which has not previously been shown, could be achieved with a boosting vaccination (Pratt et al., 2010).

17. Ebola virus recombinant vector vaccine pVSVXN2∆G/ZEBOVsGP encoding GP

a. Vaccine Ontology ID:

VO_0004381

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of GP from Zaire ebolavirus

Type: Recombinant vector construction
Description: Vector VSVXN2∆G expressed the transmembrane glycoproteins of Zaire Ebola virus (GP) (Garbutt et al., 2004).
Detailed Gene Information: Click here.

f. Vector:

VSVXN2∆G in vesicular stomatitis virus (Garbutt et al., 2004)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Efficacy: The rVSV expressing the Zaire Ebola virus transmembrane glycoprotein mediated protection in mice against a lethal Zaire Ebola virus challenge (Garbutt et al., 2004).

18. Ebola virus recombinant VSVΔG-GP encoding GP

a. Vaccine Ontology ID:

VO_0004336

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Macaque

e. Gene Engineering of GP from Zaire ebolavirus

Type: Recombinant vector construction
Description: Vector VSVΔG expressed GP from ZEBOV (Jones et al., 2005).
Detailed Gene Information: Click here.

f. Vector:

a replication competent vesicular stomatitis virus vector (VSVΔG) (Jones et al., 2005)

g. Immunization Route

Intramuscular injection (i.m.)

h. Macaque Response

Vaccine Immune Response Type: VO_0000286
Efficacy: A single intramuscular injection of the EBOV or MARV vaccine elicited completely protective immune responses in nonhuman primates against lethal EBOV or MARV challenges (Jones et al., 2005).

19. Ebola Virus Vaccine Ad5-ZGP

a. Type:

Live, attenuated vaccine

b. Status:

Licensed

c. Host Species for Licensed Use:

Baboon

d. Antigen

ZGP (Wong et al., 2015)

e. Gene Engineering of ZGP

Type: Recombinant vector construction
Description: (Wong et al., 2015)
Detailed Gene Information: Click here.

f. Vector:

Adenovirus 5

g. Immunization Route

Intramuscular injection (i.m.)

h. Description

An adenovirus vectored vaccine encoding Ebola virus ZGP providing protection in Macaque model. (Wong et al., 2015)

i. Macaque Response

Vaccination Protocol: NHPs were treated at either 30 mins or 24 hrs after exposure. Control NHPs were treated with phosphate-buffered saline. Specific treatment procedures described in articles. (Wong et al., 2015)
Immune Response: All surviving cynomolgus macaques treated 30 minutes or 24 hours after EBOV challenge had elevated anti-ZGP IgG levels by 14 days after infection, and levels remained elevated until the termination of the experiment 28 days after infection. ZGP-specific IgG was not detected in nonsurviving NHPs A4 and A8 but was detected in animal A3 by 7 days after infection. For nonsurviving and control animals in the 24-hour treatment groups, ZGP-specific IgG was not detected, with the exception of animal E2. Surviving animals in the 24-hour treatment groups developed elevated anti-ZGP IgG levels by 14 days after infection, which remained elevated 28 days after infection. (Wong et al., 2015)
Challenge Protocol: NHPs were challenged with a uniformly lethal dose of 1000 plaque-forming units of EBOV (Ebola virus H.sapiens-tc/COD/1995/Kikwit-9510621; GenBank accession number AY354458). (Wong et al., 2015)
Efficacy: Six of 9 NHPs (67%) survived the challenge when treated 30 minutes after exposure, whereas 1 of 4 (25%) survived challenge when treated 24 hours after infection. The 3 nonsurviving NHPs treated 30 minutes after challenge had a delayed time to death of 13, 11, and 13 days after infection, surviving nearly twice as long as the control animals, which died 7 days after infection. In contrast, the 3 nonsurviving cynomolgus macaques treated 24 hours after challenge died 7, 7, and 9 days after infection, which is similar to times of death for the control animals. Nonsurviving, treated rhesus macaques died 11 and 16 days after infection, which is longer than the control animals. (Wong et al., 2015)

20. GP and NP

a. Vaccine Ontology ID:

VO_0004073

b. Type:

VEEV Replicon

c. Preparation

The Ebola NP and GP genes from the Mayinga strain of Ebola virus were derived from pSP64- and pGEM3Zf(-)-based plasmids. The BamHI±EcoRI (2.3 kb) and BamHI±KpnI (2.4 kb) fragments containing the NP and GP genes, respectively, were subcloned into a shuttle vector digested with BamHI and EcoRI within a polylinker sequence flanked by ClaI sites. For cloning of the GP gene, overhanging ends produced by KpnI (in the GP fragment) and EcoRI (in the shuttle vector) were made blunt by incubation with T4 DNA polymerase. From the shuttle vector, NP or GP genes were transferred as ClaI-fragments into the ClaI site of the replicon clone, resulting in plasmids encoding the NP or GP gene in place of the VEE structural protein genes (Pushko et al., 2000).

d. Virulence

e. Description

This immunogen is composed of RNA replicon particles derived from an attenuated strain of Venezuelan equine encephalitis virus (VEEV) expressing EBOV glycoprotein and nucleoprotein (Geisbert et al., 2002).

f. Monkey Response

Host Strain: Cynomolgus macaques
Vaccination Protocol: Groups of three cynomolgus macaques were vaccinated with VRP that expressed EBOV GP, EBOV NP, a mixture of EBOV GP and EBOV NP, or a control antigen (influenza hemagglutinin) that has no effect on EBOV immunity. Animals were vaccinated by subcutaneous injection of 10^7 focus-forming units of VRP in a total of 0.5 mL at one site. Vaccinations were repeated 28 days after the first injection and 28 days after the second (Geisbert et al., 2002).
Persistence: None noted
Side Effects: None noted
Efficacy: These results indicate that rodent models of EBOV hemorrhagic fever do not consistently predict efficacy of candidate vaccines in nonhuman primates, perhaps because the disease course in rodents differs from that reported in human and nonhuman primates (Geisbert et al., 2002).
Description: All animals, including the four untreated macaques, were challenged with 1,000 PFU of EBOV 49 days after the third vaccine dose. At postchallenge day 3, all animals became ill; two animals from each vaccination group (i.e., GP, NP, GP + NP, influenza HA) died on day 6, and the remaining animals died on day 7 (Geisbert et al., 2002).

21. GP-VRP

a. Vaccine Ontology ID:

VO_0000780

b. Type:

Recombinant vector vaccine

c. Gene Engineering of GP from Reston ebolavirus

Type: Protein
Description:
Detailed Gene Information: Click here.

d. Vector:

VRP: VEE replicon particle

e. Preparation

The Ebola GP genes from the Mayinga strain of Ebola virus were derived from pGEM3Zf(-)-based plasmid. The BamHI±KpnI (2.4 kb) fragment containing the GP gene was subcloned into a shuttle vector. From the shuttle vector, GP gene was transferred as ClaI-fragment into the ClaI site of the replicon clone, resulting in plasmids encoding the GP gene in place of the VEE structural protein genes (Pushko et al., 2000).

f. Virulence

g. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: VRP were diluted in PBS and administered to 6±8 week old BALB/c mice. Groups of 10 BALB/c mice were inoculated on days 0 and 28 with two doses of NP-VRP, GP-VRP, or a mixture of both. Challenge was carried out 4 weeks after final immunization with VRP. Mice were challenged i.p. with mouse-adapted Ebola virus. To determine subsequent viral titers in the serum, liver, and spleen, two mice were taken from VRP-vaccinated or control groups on each of days 1±5 after challenge, anesthetized and exsanguinated. Portions of the liver and spleen were removed aseptically, weighed, and ground in a sterile mortar. Viral titers in the sera and tissues were determined by plaque assay (Pushko et al., 2000).
Persistence: None noted
Side Effects: None
Efficacy: GP-VRP was effective in protecting BALB/c mice against a lethal challenge with mouse-adapted Ebola virus (Pushko et al., 2000). Nine out of ten animals vaccinated with GP-VRP were protected (Pushko et al., 2000).

h. Guinea pig Response

Host Strain: strain 2 and strain 13
Vaccination Protocol: VRP were diluted in PBS and administered to inbred, strain 2 or strain 13 guinea pigs. Groups of five guinea pigs were inoculated subcutaneously (s.c.) at day 0 with a total of 0.5 ml containing 10^7 IU VRP at one (strain 2) or two (strain 13) dorsal sites. Challenge was carried out 4 weeks after final immunization with VRP. Guinea pigs were challenged s.c. with 1000 LD50 of guinea pig- adapted Ebola virus. Animals were observed daily for 60 days, and morbidity (determined as changes in behavior, appearance, and weight) and survival were recorded. Blood samples were taken
on the days indicated after challenge and viremia levels were determined by plaque assay (Pushko et al., 2000).
Persistence: None noted
Side Effects: None noted
Efficacy: At day 7 after challenge, both VRP-vaccinated groups had lower viremia titers than control animals. All mockvaccinated animals or NP-VRP-vaccinated animals became ill, and died at days 8±11 after challenge. However, three out of five guinea pigs vaccinated with GP-VRP showed no signs of illness and survived challenge, and the remaining two showed increased survival times. No clear relationship with survival and antibody titers was observed, as the pre-challenge ELISA and PRNT50 titers of the two GP-VRP-inoculated animals that died were equivalent to those of the three survivors (Pushko et al., 2000).

22. NP-VRP

a. Vaccine Ontology ID:

VO_0011387

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Gene Engineering of EBOV NP

Type: Recombinant vector construction
Description:
Detailed Gene Information: Click here.

e. Vector:

VRP: VEE replicon particle

f. Preparation

The Ebola NP gene from the Mayinga strain of Ebola virus were derived from pSP64-based plasmid. The BamHI±EcoRI (2.3 kb) fragment containing the NPgene, was subcloned into a shuttle vector digested with BamHI and EcoRI within a polylinker sequence flanked by ClaI sites. From the shuttle vector, NP gene was transferred as ClaI-fragments into the ClaI site of the replicon clone, resulting in plasmids encoding the NP gene in place of the VEE structural protein genes (Pushko et al., 2000).

g. Immunization Route

Intramuscular injection (i.m.)

h. Guinea pig Response

Host Strain: strain 2 and strain 13
Vaccination Protocol: VRP were diluted in PBS and administered to inbred, strain 2 or strain 13 guinea pigs. Groups of five guinea pigs were inoculated subcutaneously (s.c.) at day 0 with a total of 0.5 ml containing 10^7 IU VRP at one (strain 2) or two (strain 13) dorsal sites. Challenge was carried out 4 weeks after final immunization with VRP. Guinea pigs were challenged s.c. with 1000 LD50 of guinea pig- adapted Ebola virus. Animals were observed daily for 60 days, and morbidity (determined as changes in behavior, appearance, and weight) and survival were recorded. Blood samples were taken on the days indicated after challenge and viremia levels were determined by plaque assay (Pushko et al., 2000).
Efficacy: At day 7 after challenge, NP-VRP-vaccinated group had lower viremia titers than control animals. All mock vaccinated animals or NP-VRP-vaccinated animals became ill, and died at days 8±11 after challenge (Pushko et al., 2000).

i. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: VRP were diluted in PBS and administered to 6±8 week old BALB/c mice. Groups of 10 BALB/c mice were inoculated on days 0 and 28 with two doses of NP-VRP, GP-VRP, or a mixture of both. Challenge was carried out 4 weeks after final immunization with VRP. Mice were challenged i.p. with mouse-adapted Ebola virus. To determine subsequent viral titers in the serum, liver, and spleen, two mice were taken from VRP-vaccinated or control groups on each of days 1±5 after challenge, anesthetized and exsanguinated. Portions of the liver and spleen were removed aseptically, weighed, and ground in a sterile mortar. Viral titers in the sera and tissues were determined by plaque assay (Pushko et al., 2000).
Efficacy: NP-VRP was effective in protecting BALB/c mice against a lethal challenge with mouse-adapted Ebola virus (Pushko et al., 2000). All mice vaccinated with NP-VRP survived the challenge with no signs of illness (Pushko et al., 2000).

23. rAd-GP (Ebola virus)

a. Vaccine Ontology ID:

VO_0004631

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Gene Engineering of GP from Zaire ebolavirus

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Vector:

(O'Brien et al., 2014)

g. Preparation

(O'Brien et al., 2014)

h. Immunization Route

Intramuscular injection (i.m.)

i. Mouse Response

Host Strain: IFNR(-/-) mice
Vaccine Immune Response Type: VO_0003057
Immune Response: the antibody response in IFNR(-/-) mice was similar to that observed in vaccinated wild-type mice (O'Brien et al., 2014).
Efficacy: The recombinant vaccines elicited good levels of protection in the knock-out mouse (O'Brien et al., 2014).

24. rCMV- EBOV

a. Vaccine Ontology ID:

VO_0004717

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Gene Engineering of NP from Zaire Ebola virus

Type: Recombinant protein preparation
Description: A mouse CMV (MCMV) vector expressing a CD8+ T cell epitope from the nucleoprotein (NP) of Zaire ebolavirus (ZEBOV) (MCMV/ZEBOV-NP(CTL)) (Tsuda et al., 2011).
Detailed Gene Information: Click here.

f. Preparation

A mouse CMV (MCMV) vector expressing a CD8+ T cell epitope from the nucleoprotein (NP) of Zaire ebolavirus (ZEBOV) (MCMV/ZEBOV-NP(CTL)) (Tsuda et al., 2011).

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Vaccination Protocol: Mice were vaccinated with rCMV- EBOV (Tsuda et al., 2011).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: Mice were challenged with a lethal dose of ZEBOV (Tsuda et al., 2011).
Efficacy: The vaccine induced high levels of long-lasting (>8 months) CD8+ T cells against ZEBOV NP in mice. Importantly, all vaccinated animals were protected against lethal ZEBOV challenge (Tsuda et al., 2011).

25. rVEE-Ebola-NP

a. Vaccine Ontology ID:

VO_0004805

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Antigen

Ebola virus nucleoprotein (NP) (Wilson and Hart, 2001)

f. Gene Engineering of NP from Zaire Ebola virus

Type: Recombinant vector construction
Description:
Detailed Gene Information: Click here.

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Host Strain: C57BL/6
Vaccine Immune Response Type: VO_0003057
Efficacy: C57BL/6 mice vaccinated with Venezuelan equine encephalitis virus replicons encoding the Ebola virus nucleoprotein (NP) survived lethal challenge with Ebola virus (Wilson and Hart, 2001).

26. rVSV- SEBOV-GP and -VP40

a. Vaccine Ontology ID:

VO_0004661

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Gene Engineering of NP from Zaire Ebola virus

Type: Recombinant vector construction
Description: New vectors were generated that contain EBOV viral protein 40 (VP40) or EBOV nucleoprotein (NP) as a second antigen expressed by the same rVSV vector that encodes the heterologous GP (Marzi et al., 2011).
Detailed Gene Information: Click here.

f. Gene Engineering of VP40 from Zaire ebolavirus

Type: Recombinant vector construction
Description: New vectors were generated that contain EBOV viral protein 40 (VP40) or EBOV nucleoprotein (NP) as a second antigen expressed by the same rVSV vector that encodes the heterologous GP (Marzi et al., 2011).
Detailed Gene Information: Click here.

g. Preparation

rVSV-expressing SEBOV-GP and -VP40 (Marzi et al., 2011; Falzarano et al., 2011).

h. Immunization Route

Intramuscular injection (i.m.)

i. Guinea pig Response

Vaccination Protocol: The guinea pigs were vaccinated intraperitoneally with a single dose of 2 ×10^5 PFU to guinea pigs of rVSV (Marzi et al., 2011).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: The guinea pigs were subsequently challenged with 1000 LD50 of GPA-ZEBOV [20] or boosted with the same dose of rVSV and challenged 3 weeks later (Marzi et al., 2011).
Efficacy: After applying a 2-dose immunization approach, we observed an improved cross-protection rate, with 5 of 6 guinea pigs surviving the lethal ZEBOV challenge if vaccinated with rVSV-expressing SEBOV-GP and -VP40 (Marzi et al., 2011).

27. rVSV-EBOV

a. Vaccine Ontology ID:

VO_0004660

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Gene Engineering of GP from Zaire ebolavirus

Type: Recombinant vector construction
Description: The recombinant vesicular stomatitis virus (rVSV) vector-based monovalent vaccine platform expressing a glycoprotein of Zaire ebolavirus (ZEBOV) (Falzarano et al., 2011).
Detailed Gene Information: Click here.

f. Gene Engineering of GP from Cote d'Ivoire Ebola virus

Type: Recombinant vector construction
Description: The recombinant vesicular stomatitis virus (rVSV) vector-based monovalent vaccine platform expressing a glycoprotein of Côte d’Ivoire ebolavirus (CIEBOV) (Falzarano et al., 2011).
Detailed Gene Information: Click here.

g. Preparation

A recombinant vesicular stomatitis virus (rVSV) vector-based monovalent vaccine platform expressing a filovirus glycoprotein (Falzarano et al., 2011).

h. Immunization Route

Intramuscular injection (i.m.)

i. Macaque Response

Vaccination Protocol: Cynomolgus macaques were vaccinated with an rVSV vaccine expressing either the glycoprotein of Zaire ebolavirus (ZEBOV) or Côte d'Ivoire ebolavirus (CIEBOV) (Falzarano et al., 2011).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: The macaques were challenged with Bundibugyo ebolavirus (BEBOV)(Falzarano et al., 2011).
Efficacy: A single vaccination with the ZEBOV-specific vaccine provided cross-protection (75% survival) against subsequent BEBOV challenge, whereas vaccination with the CIEBOV-specific vaccine resulted in an outcome similar to mock-immunized animals (33% and 25% survival, respectively) (Falzarano et al., 2011).

28. V920

a. Manufacturer:

BioProtection Systems Corporation and Department of Health and Human Services

b. Type:

Recombinant vector vaccine

c. Status:

Clinical trial

d. Host Species for Licensed Use:

Human

e. Immunization Route

Intramuscular injection (i.m.)

29. VRP expressing VP24

a. Vaccine Ontology ID:

VO_0000781

b. Type:

VEEV replicon

c. Gene Engineering of VP24 from Zaire ebolavirus

Type: Protein
Description:
Detailed Gene Information: Click here.

d. Vector:

VRP: virus-like replicon particle

e. Preparation

Replicon RNAs were packaged into particles. Briefly, capped replicon RNAs were produced in vitro by T7 runoff transcription of NotI-digested plasmid templates using the RiboMAX T7 RNA polymerase kit. BHK cells were cotransfected with the replicon RNAs and two RNAs expressing the VEE virus structural proteins. The cell culture supernatants were harvested approximately 30 h after transfection and the replicon particles were concentrated and partially purified by centrifugation through a 20% sucrose cushion. Packaged VRPs were suspended in phosphatebuffered saline and titers were determined as immunofluorescent foci after infection of Vero cells as described using either EBOV-immune rabbit serum or mouse monoclonal antibodies to VP24 (Z-AC01-BG11-01), VP35 (M-HC01-AF11), or VP40 (M-HD06-AD10) (Wilson et al., 2001).

f. Virulence

g. Description

VP24 is an Ebola virus protein. It is membrane associated and is most likely located on the inside of the membrane. The function of VP24 is not known but it may serve as a minor matrix protein, facilitating the interaction of VP40 and/or GP with the RNP complex, or function in the uncoating of the virion during infection (Wilson et al., 2001).

h. Mouse Response

Host Strain: BALB/c and C57BL/6
Vaccination Protocol: Groups of 10 BALB/c or C57BL/6 mice per experiment were subcutaneously injected at the base of the neck with 2(10^6) focus-forming units of VRPs encoding the EBOV-Z genes, or with a control replicon encoding the Lassa N gene. Booster immunizations were administered at 1-month intervals (Wilson et al., 2001).
Persistence: None noted
Side Effects: None noted
Efficacy: Vaccination with VRPs encoding the EBOV-Z VP24 protein protected the majority (90±95%) of the BALB/c mice from lethal EBOV challenge. In a similar experiment, two inoculations of VRPs encoding the EBOV-Z VP24 protein also protected 5/5 BALB/c mice from a 3(10^4) LD50 challenge dose and 5/5 BALB/c mice from a 3(10^6) LD50 challenge dose. None of the C57BL/6 mice were protected. Most of the mice had detectable EBOV-Z-specific serum antibodies after vaccination with VRPs encoding the EBOV-Z VP protein (Wilson et al., 2001). These results indicate that the VP24 protein may be an important component of a vaccine designed to protect humans from Ebola hemorrhagic fever.

30. VRP expressing VP30

a. Vaccine Ontology ID:

VO_0000782

b. Type:

Recombinant vector vaccine

c. Gene Engineering of VP30 from Zaire ebolavirus

Type: Protein
Description:
Detailed Gene Information: Click here.

d. Vector:

VRP: virus-like replicon particle

e. Preparation

f. Virulence

g. Description

VP30 is an Ebola virus protein. It associates with the genomic RNA in a ribonucleoprotein complex. The VP30 protein is not essential for replication, but it is necessary for efficient transcription in this system. It has also recently been shown to be essential for the recovery of infectious EBOV-Z from cloned cDNAs (Wilson et al., 2001).

h. Mouse Response

Host Strain: BALB/c and C57BL/6
Vaccination Protocol: Groups of 10 BALB/c or C57BL/6 mice per experiment were subcutaneously injected at the base of the neck with 2(10^6) focus-forming units of VRPs encoding the EBOV-Z genes, or with a control replicon encoding the Lassa N gene. Booster immunizations were administered at 1-month intervals (Wilson et al., 2001).
Persistence: None noted
Side Effects: None noted
Efficacy: Three injections of VRPs encoding EBOV-Z VP30 induced protection from lethal disease in 85% of the BALB/c mice examined. However, when the vaccination schedule was decreased to two injections, only 55% of the mice immunized with VP30 survived challenge. None of the C57BL/6 mice were protected. Most of the mice had detectable EBOV-Z-specific serum antibodies after vaccination with VRPs encoding the EBOV-Z VP protein (Wilson et al., 2001). These results indicate that the VP30 protein may be an important component of a vaccine designed to protect humans from Ebola hemorrhagic fever (Wilson et al., 2001).

31. VRP expressing VP35

a. Vaccine Ontology ID:

VO_0000783

b. Type:

Recombinant vector vaccine

c. Gene Engineering of VP35 from Zaire ebolavirus

Type: Protein
Description:
Detailed Gene Information: Click here.

d. Vector:

VRP: virus-like replicon particle

e. Preparation

f. Virulence

g. Description

VP35 is an Ebola virus protein. It associates with the genomic RNA in a ribonucleoprotein complex. It is essential for replication and encapsidation of the EBOV genome. The VP35 protein has also recently been shown to be essential for the recovery of infectious EBOV-Z from cloned cDNAs. In addition to being an essential component of the replication complex, VP35 was also recently implicated as an interferon antagonist. VP35 may therefore facilitate viral replication in infected cells by blocking the induction of antiviral immune responses normally induced by the production of interferon (Wilson et al., 2001).

h. Mouse Response

Host Strain: BALB/c and C57BL/6
Vaccination Protocol: Groups of 10 BALB/c or C57BL/6 mice per experiment were subcutaneously injected at the base of the neck with 2(10^6) focus-forming units of VRPs encoding the EBOV-Z genes, or with a control replicon encoding the Lassa N gene. Booster immunizations were administered at 1-month intervals (Wilson et al., 2001).
Persistence: None noted
Side Effects: None noted
Efficacy: The VP35 protein was not efficacious in the BALB/c mouse model, as only 20 and 26% of the mice were protected from lethal challenge after either two or three doses, respectively. The mean day of death of the VP-vaccinated mice that succumbed to the EBOV challenge was within 1 day of the control Lassa N-vaccinated mice. C57BL/6 mice were protected from lethal EBOV challenge after vaccination with the EBOV-Z VP35 protein, with 70% of the mice protected after three inoculations. When the viral titers were measured 5 days after challenge, vaccination with VRPs encoding the EBOV-Z VP35 protein reduced the viral load by at least 4 log10 compared to control mice. Most of the mice had detectable EBOV-Z-specific serum antibodies after vaccination with VRPs encoding the EBOV-Z VP protein (Wilson et al., 2001). These results indicate that the VP35 protein may be an important component of a vaccine designed to protect humans from Ebola hemorrhagic fever (Wilson et al., 2001).

32. VRP expressing VP40

a. Vaccine Ontology ID:

VO_0000784

b. Type:

Recombinant vector vaccine

c. Gene Engineering of VP40 from Zaire ebolavirus

Type: Protein
Description:
Detailed Gene Information: Click here.

d. Vector:

VRP: virus-like replicon particle

e. Preparation

f. Virulence

g. Description

VP40 is an Ebola virus protein. It is membrane-associated and is most likely located on the inside of the membrane. VP40 has been shown to associate with cell membranes, where it is believed to be involved in maturation of the virus by inducing viral assembly at the plasma membrane of infected cells (Wilson et al., 2001).

h. Mouse Response

Host Strain: BALB/c and C57BL/6
Vaccination Protocol: Groups of 10 BALB/c or C57BL/6 mice per experiment were subcutaneously injected at the base of the neck with 2 x10^6 focus-forming units of VRPs encoding the EBOV-Z genes, or with a control replicon encoding the Lassa N gene. Booster immunizations were administered at 1-month intervals (Wilson et al., 2001).
Persistence: None noted
Side Effects: None noted
Efficacy: Vaccination with VRPs encoding the VP40 protein protected 85 and 70% of the BALB/c mice after either two or three injections, respectively. None of the C57BL/6 mice were protected, however most of the mice had detectable EBOV-Z-specific serum antibodies after vaccination with VRPs encoding the EBOV-Z VP protein (Wilson et al., 2001). These results indicate that the VP30 protein may be an important component of a vaccine designed to protect humans from Ebola hemorrhagic fever.

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