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Streptococcus pyogenes

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
    4. Microbial Pathogenesis
    5. Host Ranges and Animal Models
    6. Host Protective Immunity
  2. Vaccine Related Pathogen Genes
    1. emm (Protective antigen)
    2. emm1 (Protective antigen)
    3. emm12 mutant (Protective antigen)
    4. emm3 (Protective antigen)
    5. fbaA (Protective antigen)
    6. fbaA (Protective antigen)
    7. fbp54 (Protective antigen)
    8. hypothetical protein MGAS2096_Spy1592 (Protective antigen)
    9. hypothetical protein SPy_0130 (Protective antigen)
    10. hypothetical protein SPy_0843 (Protective antigen)
    11. inlA (Protective antigen)
    12. isp2 (Protective antigen)
    13. M1 (Protective antigen)
    14. M14 (Protective antigen)
    15. M18 (Protective antigen)
    16. M22 (Protective antigen)
    17. M28 (Protective antigen)
    18. M6 (Protective antigen)
    19. maltodextrin-binding proteinSPy1294 (Protective antigen)
    20. mrp (Protective antigen)
    21. mtsA (Protective antigen)
    22. nuclease (Protective antigen)
    23. oppA (Protective antigen)
    24. putative protease maturation proteinSpy1390 (Protective antigen)
    25. putative secreted protein SPy0019 (Protective antigen)
    26. putative surface exclusion protein SPy0269 (Protective antigen)
    27. putative transcriptional antiterminator (BglG family)SPy1325 (Protective antigen)
    28. SCPA (Protective antigen)
    29. scpA (Protective antigen)
    30. scpA (Protective antigen)
    31. SfbI (Protective antigen)
    32. shp (Protective antigen)
    33. sib35 (Protective antigen)
    34. slo (Protective antigen)
    35. spa (Protective antigen)
    36. spa (Protective antigen)
    37. speA (Protective antigen)
    38. speB (Protective antigen)
    39. Spy0416 (Protective antigen)
    40. Spy0469 (Protective antigen)
    41. srtA (Protective antigen)
    42. StreptInCor (Protective antigen)
    43. tmbC (Protective antigen)
  3. Vaccine Related Host Genes
    1. IgA
    2. IgG
  4. Vaccine Information
    1. Group A streptococcal recombinant fusion peptide Vaccine
    2. S. pyogenes FbaA Protein Vaccine
    3. S. pyogenes FBP54 Protein Vaccine
    4. S. pyogenes M Protein Vaccine
    5. S. pyogenes SfbI Protein Vaccine
    6. S. pyogenes Sib35 Protein Vaccine
    7. SL3261 expressing streptococcal M protein
  5. References
I. General Information
1. NCBI Taxonomy ID:
1314
2. Disease:
Group A streptococcal infections, Strep throat, Scarlet fever
3. Introduction
Streptococcus pyogenes is a spherical gram-positive bacterium that grows in long chains and is the cause of Group A streptococcal infections. S. pyogenes displays streptococcal group A antigen on its cell wall. S. pyogenes typically produces large zones of beta-hemolysis (the complete disruption of erythrocytes and the release of hemoglobin) when cultured on blood agar plates and are therefore also called Group A (beta-hemolytic) Streptococcus (abbreviated GAS). S. pyogenes is the cause of many important human diseases ranging from mild superficial skin infections to life-threatening systemic diseases. Infections typically begin in the throat or skin. Examples of mild S. pyogenes infections include pharyngitis ("strep throat") and localized skin infection ("impetigo"). Erysipelas and cellulitis are characterized by multiplication and lateral spread of S. pyogenes in deep layers of the skin. S. pyogenes invasion and multiplication in the fascia can lead to necrotizing fasciitis, a potentially life-threatening condition requiring surgical treatment (Wiki: S. pyogenes).
4. Microbial Pathogenesis
Colonization of tissues by S. pyogenes is thought to result from a failure in the constitutive defenses (normal flora and other nonspecific defense mechanisms) which allows establishment of the bacterium at a portal of entry (often the upper respiratory tract or the skin) where the organism multiplies and causes an inflammatory purulent lesion (Textbook of Bacteriology).
5. Host Ranges and Animal Models
S. pyogenes is a human pathogen and mice are the animal model that is used (Okamoto et al., 2005).
6. Host Protective Immunity
Macrophages and the antibody mediated response are the major defenses for host protective immunity against S. pyogenes infection (Textbook of Bacteriology).
1. emm
  • Gene Name : emm
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : AAN04081
  • Other Database IDs : CDD:235600
    CDD:274008
  • Taxonomy ID : 1314
  • Protein Name : M24
  • Protein pI : 5.13
  • Protein Weight : 23639.74
  • Protein Length : 278
  • Protein Note : V-type ATP synthase subunit I; Validated
  • Protein Sequence : Show Sequence 
    >AAN04081.1 M24, partial [Streptococcus pyogenes]
NGFVAVALTVLGAGLVVNTNEVSAVATRSQTNTLEKVQERADKFEIENNTLKLKNSDLSFNNKVLKDHND
ELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKA
DLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKA
DLEKALEGAMNFS
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (McNeil et al., 2005)
2. emm1
  • Gene Name : emm1
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • VO ID : VO_0012397
  • NCBI Nucleotide GI : 311757
  • NCBI Protein GI : 311758
  • Protein Accession : CAA44062.1
  • 3D structure: PDB ID : 2OTO
  • Other Database IDs : CDD:273479
    CDD:291694
    CDD:295219
    CDD:279132
    GOA:Q10372
    HSSP: 1IC2
    InterPro: IPR001899
    InterPro: IPR005877
    InterPro: IPR019931
    InterPro: IPR019948
    InterPro: IPR019950
    UniProtKB/TrEMBL: Q10372
  • Taxonomy ID : 1314
  • Gene Strand (Orientation) : ?
  • Protein Name : M protein type 1
  • Protein pI : 6.86
  • Protein Weight : 52910.57
  • Protein Length : 546
  • Protein Note : Gram-positive signal peptide, YSIRK family; TIGR01168
  • DNA Sequence : Show Sequence 
    >gi|311757|emb|X62131.1| S.pyogenes emm1 (29/58) gene for M protein type 1
GGATCCAATGATAACATAAGGAGCATAAAAATGGCTAAAAATAACACGAATAGACACTATTCGCTTAGAA
AATTAAAAACAGGAACGGCTTCAGTAGCGGTAGCTTTGACTGTTTTAGGGGCAGGTTTTGCGAATCAAAC
AGAGGTTAAGGCTAACGGTGATGGTAATCCTAGGGAAGTTATAGAAGATCTTGCAGCAAACAATCCCGCA
ATACAAAATATACGTTTACGTCACGAAAACAAGGACTTAAAAGCGAGATTAGAGAATGCAATGGAAGTTG
CAGGAAGAGATTTTAAGAGAGCTGAAGAACTTGAAAAAGCAAAACAAGCCTTAGAAGACCAGCGTAAAGA
TTTAGAAACTAAATTAAAAGAACTACAACAAGACTATGACTTAGCAAAGGAATCAACAAGTTGGGATAGA
CAAAGACTTGAAAAAGAGTTAGAAGAGAAAAAGGAAGCTCTTGAATTAGCGATAGACCAGGCAAGTCGGG
ACTACCATAGAGCTACCGCTTTAGAAAAAGAGTTAGAAGAGAAAAAGAAAGCTCTTGAATTAGCGATAGA
CCAAGCGAGTCAGGACTATAATAGAGCTAACGTCTTAGAAAAAGAGTTAGATACGATTACTAGAGAACAA
GAGATTAATCGTAATCTTTTAGGCAATCGAAAACTTGAACTTGATCAACTTTCATCTGAAAAAGAGCAGC
TAACGATCGAAAAAGCAAAACTTGAGGAAGAAAAACAAATCTCAGACGCAAGTCGTCAAAGCCTTCGTCG
TGACTTGGACGCATCACGTGAAGCTAAGAAACAGGTTGAAAAAGATTTAGCAAACTTGACTGCTGAACTT
GATAAGGTTAAAGAAGACAAACAAATCTCAGACGCAAGCCGTCAAGGCCTTCGCCGTGACTTGGACGCAT
CACGTGAAGCTAAGAAACAGGTTGAAAAAGATTTAGCAAACTTGACTGCTGAACTTGATAAGGTTAAAGA
AGAAAAACAAATCTCAGACGCAAGCCGTCAAGGCCTTCGCCGTGACTTGGACGCATCACGTGAAGCTAAG
AAACAAGTTGAAAAAGCTTTAGAAGAAGCAAACAGCAAATTAGCTGCTCTTGAAAAACTTAACAAAGAGC
TTGAAGAAAGCAAGAAATTAACAGAAAAAGAAAAAGCTGAACTACAAGCAAAACTTGAAGCAGAAGCAAA
TGTACTTAAAGATCCATTAGCGAAACAAGCTGAAGAACTCGCAAAACTAAGAGCTGGAAAAGCATCAGAC
TCACAAACCCCTGATACAAAACCAGGAAACAAAGCTGTTCCAGGTAAAGGTCAAGCACCACAAGCAGGTA
CAAAACCTAACCAAAACAAAGCACCAATGAAGGAAACTAAGAGACAGTTACCATCAACAGGTGAAACAGC
TAACCCATTCTTCACAGCGGCAGCCCTTACTGTTATGGCAACAGCTGGAGTAGCAGCAGTTGTAAAACGC
AAAGAAGAAAACTAAGCTGAATTC
  • Protein Sequence : Show Sequence 
    >CAA44062.1 M protein type 1 [Streptococcus pyogenes]
MAKNNTNRHYSLRKLKTGTASVAVALTVLGAGFANQTEVKANGDGNPREVIEDLAANNPAIQNIRLRHEN
KDLKARLENAMEVAGRDFKRAEELEKAKQALEDQRKDLETKLKELQQDYDLAKESTSWDRQRLEKELEEK
KEALELAIDQASRDYHRATALEKELEEKKKALELAIDQASQDYNRANVLEKELDTITREQEINRNLLGNR
KLELDQLSSEKEQLTIEKAKLEEEKQISDASRQSLRRDLDASREAKKQVEKDLANLTAELDKVKEDKQIS
DASRQGLRRDLDASREAKKQVEKDLANLTAELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKALEEA
NSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEANVLKDPLAKQAEELAKLRAGKASDSQTPDTKPGN
KAVPGKGQAPQAGTKPNQNKAPMKETKRQLPSTGETANPFFTAAALTVMATAGVAAVVKRKEEN
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice were intranasally immunized with diphtheria toxoid (DT) conjugated polypeptides encompassing a conformational-constrained B cell epitope of the M1 protein (J8). Vaccinated mice were challenged 10 days after the last boost by the intranasal route. Animals had 60% protection against challenge (Schulze et al., 2006).
  • Related Vaccine(s): S. pyogenes M Protein Vaccine
3. emm12 mutant
  • Gene Name : emm12 mutant
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : BAD14986
  • Other Database IDs : ATCC:12353
    CDD:273479
    CDD:302792
    CDD:303051
    CDD:287450
    CDD:295219
  • Taxonomy ID : 1314
  • Protein Name : M12 mutant protein precursor
  • Protein pI : 8.53
  • Protein Weight : 55130.42
  • Protein Length : 583
  • Protein Note : Gram-positive signal peptide, YSIRK family; TIGR01168
  • Protein Sequence : Show Sequence 
    >BAD14986.1 M12 mutant protein precursor [Streptococcus pyogenes]
MAKNTTNRHYSLRKLKTGTASVAVALTVVGAGLVAGQTVRADHSDLVAEKQRLEDLGQKFERLKQRSELY
LQQYYDNKSNGYKGDWYVQQLKMLNRDLEQAYNELSGEAHKDALGKLGIDNADLKAKITELEKSVEEKND
VLSQIKKELEEAEKDIQFGREVHAADLLRHKQEIAEKENVISKLNGELQPLKQKVDETDRNLQQEKQKVL
SLEQQLAVTKENAKKDFELAALGHQLADKEYNAKIAELESKLADAKKDFELAALGHQHAHNEYQAKLAEK
DGQIKQLEEQKQILDASRKGTARDLEAVRQAKKATEAELNNLKAELAKVTEQKQILDASRKGTARDLEAV
RKAKAQVEAALKQLEEQNKISEASRKGLRRDLDASREAKKQVEKDLANLTAELDKVKEEKQISDASRQGL
RRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALKEQLAKQAEE
LAKLRAGVAAVVKRKEEN
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (McNeil et al., 2005)
4. emm3
  • Gene Name : emm3
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : CAA56449
  • Other Database IDs : CDD:273479
    CDD:303051
    CDD:287450
    CDD:287215
    CDD:279132
    GOA:Q54835
    InterPro: IPR001899
    InterPro: IPR005877
    InterPro: IPR019931
    InterPro: IPR019948
    InterPro: IPR019950
    UniProtKB/TrEMBL: Q54835
  • Taxonomy ID : 1314
  • Protein Name : M3 protein
  • Protein pI : 9.26
  • Protein Weight : 62443.99
  • Protein Length : 639
  • Protein Note : Gram-positive signal peptide, YSIRK family; TIGR01168
  • Protein Sequence : Show Sequence 
    >CAA56449.1 M3 protein [Streptococcus pyogenes]
MAKNNTNRHYSLRKLKTGTASVAVALTVLGTGLVAGQTVKADARSVNGEFPRHVKLKNEIENLLDQVTQL
YNKHNSNYQQYSAQAGRLDLRQKAEYLKGLNDWAERLLQELNGEDVKKVLGKVAFEKDDLEKEVKELKEK
IDKKEKEYQDLDKDFDLAKQGYVLSDKRHQQELEEKEKKVTEATAKVGQISEELETVKQKVESTMQDLTE
KQNRVSQLEQELATTKQNAKEDFELAALANAADKQKLEAKIADLETKLKEAKEDFELAALGHQHAHNEYQ
AKLAEKDDQIKQLEEQKQILDASRKGTARDLEAVRQAKKATEAELNNLKAELAKVTEQKQILDASRKGTA
RDLEAVRQAKAQVEAALKQLEEQNRISEASRKGLRRDLDASREAKKQVEKDLANLTAELDKVKEEKQISD
ASRQGLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEARALKEQL
AKQAEELAKLRAGKASDSQIPDTKPGNKAVPGKGQAPQAGTKPNQNKAPMKETKRQLPSTGETANPFFTA
AALTVMATAGVAAVVKRKEEN
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (McNeil et al., 2005)
5. fbaA
  • Gene Name : fbaA
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes SSI-9
  • VO ID : VO_0012396
  • NCBI Protein GI : 14915682
  • Other Database IDs : CDD:273479
    CDD:320995
    CDD:273478
  • Taxonomy ID : 1314
  • Gene Strand (Orientation) : ?
  • Protein Name : fibronectin-binding protein
  • Protein pI : 4.73
  • Protein Weight : 36233.27
  • Protein Length : 427
  • Protein Note : Gram-positive signal peptide, YSIRK family; TIGR01168
  • Protein Sequence : Show Sequence 
    >BAB62098.1 fibronectin-binding protein [Streptococcus pyogenes]
MRRAENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDGIPPISLTQKTTATTSENWHHIDKDGLIPLG
ISLEAAKEEFKKEVEESRLSEAQKETYKQKIKTAPDKDKLLFTYHSEYMTAVKDLPASTESTTQPVEAPV
QETQASASDSMVTGDSTSVTTDSPEETPSSESPVAPALSEAPAQPAESEEPSVAASSEETPSPSTPAAPS
TPAAPETPEEPAAPSQPAESEESSVAATTSPSPSTPAESETQTPPAVTKDSDKPSSAAEKPAASSLVSEQ
TVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVRAHESGKYLPSTGEKAQPLFIATMTLMSLFGSLLVTKRQ
KETKK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Mice immunized with FbpA were protected from challenge with group A streptococcus (Ma et al., 2009).
  • Related Vaccine(s): S. pyogenes FbaA Protein Vaccine
6. fbaA
  • Gene Name : fbaA
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : AMY98237
  • Other Database IDs : CDD:273479
    CDD:331285
    CDD:273478
  • Taxonomy ID : 1314
  • Protein Name : Fibronectin-binding protein
  • Protein pI : 4.24
  • Protein Weight : 39274.04
  • Protein Length : 455
  • Protein Note : Gram-positive signal peptide, YSIRK family; TIGR01168
  • Protein Sequence : Show Sequence 
    >AMY98237.1 Fibronectin-binding protein [Streptococcus pyogenes]
MFRVENNKHSRYSIRKLSVGVTSIAIASFFLGKVAYAVDTVQENTANTSQKWYDINQHGIIPVGISFEAA
KEDFKKEVEESRLSKEQKEFYNQKIKTAPDKDELLFTYHSEYMTAVKNLPTSTEPVEASVQETQASVSDS
MVTDDSTSVTTDSPEETPSPETPEAPVAPSPSIPEVPETPALSEAPAQPAESEEPSVAAPSEEAPSPSTP
EEPAAPAPSPESEEPSVAAPSEETPSPETPAAPEIPAESEAPAQPAESEESSVAATTSPSPSTPAESETQ
TPPAVTKDSDKPSSAAEKPAASSLVSEQTVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVMAHESGKYLPS
TGEKAQPLFIATMTLMSLFGSLLVTKRQKETKK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Ma et al., 2014)
7. fbp54
  • Gene Name : fbp54
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : AAR18990
  • Other Database IDs : CDD:224212
    CDD:283353
  • Taxonomy ID : 1314
  • Protein Name : fibronectin/fibrinogen-binding protein
  • Protein pI : 8.52
  • Protein Weight : 52434.05
  • Protein Length : 567
  • Protein Note : Predicted component of the ribosome quality control (RQC) complex, YloA/Tae2 family, contains fibronectin-binding (FbpA) and DUF814 domains [Translation, ribosomal structure and biogenesis]; COG1293
  • Protein Sequence : Show Sequence 
    >AAR18990.1 fibronectin/fibrinogen-binding protein, partial [Streptococcus pyogenes]
MIMRKYLQGAVIEQLEQIDNDRIIEIKVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHV
GFSQNSYRTILPGSTYIEPPKTAAVNPFTITDVPLFEILQTQELTVKSLQQHFQGLGRDTAKELAELLTT
DKLKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSDMLDHFYQDKAERDRINQQASDLIHRVQTEL
DKNRNKLSKQEAELLATENAELFRQKGELLTTYLSMVPNNQDSVVLDNYYTGEKLEIALDKALTPNQNAQ
RYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYNLSQASIDDIEDIREELYQAGFLKSRQRDKRHKRK
KPEQYLASDGKTILMVGRNNLQNEELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAY
YSKARLSNLVQVDMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLF
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Kawabata et al., 2001)
  • Related Vaccine(s): S. pyogenes FBP54 Protein Vaccine
8. hypothetical protein MGAS2096_Spy1592
  • Gene Name : hypothetical protein MGAS2096_Spy1592
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes M1 GAS
  • NCBI Protein GI : ABF36644
  • Taxonomy ID : 370553
  • Protein Name : hypothetical protein
  • Protein pI : 9.79
  • Protein Weight : 18121.19
  • Protein Length : 251
  • Protein Sequence : Show Sequence 
    >ABF36644.1 hypothetical protein MGAS2096_Spy1592 [Streptococcus pyogenes MGAS2096]
MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGFFYLQSQLKNEAFRAQLTTEQLAQLQSSMTPFMIF
LSVLNVLAIIAIIIFCTQNLSKLKQGLTVSYIPYILRLILSVIGLVNQFTTTLSMVGTILILIQAALYGF
AFYKAKTLNEKGDDTDQGYAVIK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Lei et al., 2004)
9. hypothetical protein SPy_0130
  • Gene Name : hypothetical protein SPy_0130
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : NP_268519
  • Other Database IDs : CDD:274781
  • Taxonomy ID : 160490
  • Protein Name : hypothetical protein
  • Protein pI : 5.14
  • Protein Weight : 23345.68
  • Protein Length : 295
  • Protein Note : streptococcal pilin isopeptide linkage domain; TIGR03786
  • Protein Sequence : Show Sequence 
    >NP_268519.1 hypothetical protein SPy_0130 [Streptococcus pyogenes M1 GAS]
MKKSILRILAIGYLLMSFCLLDSVEAENLTASINIEVINQVDVATNKQSSDIDETFMFVIEALDKESPLP
NSVTTSVKGNGKTSFEQLTFSEVGQYHYKIHQLLGKNSQYHYDETVYEVVIYVLYNEQSGALETNLVSNK
LGETEKSELIFKQEYSEKTPEPHQPDTTEKEKPQKKRNGILPSTGEMVSYVSALGIVLVATITLYSIYKK
LKTSK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Severin et al., 2007)
10. hypothetical protein SPy_0843
  • Gene Name : hypothetical protein SPy_0843
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes M1 GAS
  • NCBI Protein GI : NP_269051
  • Other Database IDs : CDD:315882; CDD:275383
  • Taxonomy ID : 160490
  • Protein Name : hypothetical protein
  • Protein pI : 6.61
  • Protein Weight : 107071.72
  • Protein Length : 1099
  • Protein Note : Leucine rich repeats (6 copies); pfam13306
  • Protein Sequence : Show Sequence 
    >NP_269051.1 hypothetical protein SPy_0843 [Streptococcus pyogenes M1 GAS]
MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISGADYAESSGKSKLKINETSGPVDDTVT
DLFSDKRTTPEKIKDNLAKGPREQELKAVTENTESEKQITSGSQLEQSKESLSLNKTVPSTSNWEICDFI
TKGNTLVGLSKSGVEKLSQTDHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVD
GKEIINEGEVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDLPDNL
KAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASFQDNDLSQLMLPDGLEK
IESEAFTGNPGDDHYNNRVVLWTKSGKNPSGLATENTYVNPDKSLWQESPEIDYTKWLEEDFTYQKNSVT
GFSNKGLQKVKRNKNLEIPKQHNGVTITEIGDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSN
NLKSFEASDDLEEIKEGAFMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANN
LIFMGSKVKTLGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKKNHLKQL
EVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLSSTIVDLEKILKLIEGLDY
STLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQFFLGRVDLDKAIAKAEKALVTKKATKNGQL
LERSINKAVLAYNNSAIKKANVKRLEKELDLLTGLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVY
FDKSGKLIYALDMSDTIGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLSQLTSIR
QVPTAAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRGLQSNPKTNRGRHSAILPRTGS
KGSFVYGILGYTSVALLSLITAIKKKKY
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Severin et al., 2007)
11. inlA
  • Gene Name : inlA
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes M1 GAS
  • NCBI Protein GI : NP_269467
  • Other Database IDs : CDD:331999
    CDD:275380
    CDD:227223
  • Taxonomy ID : 160490
  • Protein Name : putative internalin A precursor
  • Protein pI : 6.3
  • Protein Weight : 83906.01
  • Protein Length : 882
  • Protein Note : Streptococcal histidine triad protein; cl27178
  • Protein Sequence : Show Sequence 
    >NP_269467.1 putative internalin A precursor [Streptococcus pyogenes M1 GAS]
MKTKKVIILVGLLLSSQLTLIACQSRGNGTYPIKTKQSRKGMTSNKIKPIKKSKKTNKTHKGVAGVDFPT
DDGFILTKDSKILSKTDQGIVVDHDGHSHFIFYADLKGSPFEYLIPKGASLAKPAVAQRAASQGTSKVAD
PHHHYEFNPADIVAEDALGYTVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSSLVSTATANGIPGLH
FPTSDGFQFNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAHVADQYDPAKKAEKPAETHQTPELSE
REKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIGKDIPDPHAIEHARELE
KHKVGMDTLRALGFDEEVILDIVRTHDAPTPFPSNEKDPNMMKEWLATVIKLDLGSRKDPLQRKGLSLLP
NLETLGIGFTPIKDISPVLQFKKLKQLLMTKTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTL
VAAADNGIEDIRPLGQLPNLKFLVLSNNKISDLSPLASLHQLQELHIDNNQITDLSPVSHKESLTVVDLS
RNADVDLATLQAPKLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLQSLEGIEASSVIVRVEAEGNQIK
SLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPNKTVTNIDISHNNISLADL
KLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAKESAQEASESHDYNHNHTYEDEEGHAHEHRD
KDDHDHEHEDENEAKDEQNHAD
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Bensi et al., 2012)
12. isp2
  • Gene Name : isp2
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : NP_269810
  • Other Database IDs : CDD:330579
    CDD:226451
  • Taxonomy ID : 160490
  • Gene Strand (Orientation) : ?
  • Protein Name : immunogenic secreted precursor-like protein
  • Protein pI : 6.13
  • Protein Weight : 52821.45
  • Protein Length : 601
  • Protein Note : Domain of unknown function (DUF4775); cl25758
  • Protein Sequence : Show Sequence 
    >NP_269810.1 immunogenic secreted precursor-like protein [Streptococcus pyogenes M1 GAS]
MNKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSDSSSKEENNKNAAPTTSDKTDQGPLDA
SAETNSNSLVNADDKKRSDSSQSAIGSSDNKAEAENQVDDKSTDHSKSTDHSKPTDQPKPSPSKVDTAPA
SSLSKQLPEARTPIQSLSPYVSDLDLSEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYL
KSTGIAYDSTRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAFDLDPTQASKF
NDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFTDTTGSGKRRAQIMEDLDKWIDD
HGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLGYQASSYAWGQCTWYVYNRAKELGYQFDPFMGNGG
DWKYKVGYALSKTPKVGYAISFAPGQAGADGTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQ
AEQLTYVIGKSKN
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Mortensen et al., 2016)
13. M1
  • Gene Name : M1
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : AAA85117
  • Other Database IDs : CDD:236892
  • Taxonomy ID : 1314
  • Protein Name : M1 protein
  • Protein pI : 7
  • Protein Weight : 11420.13
  • Protein Length : 160
  • Protein Note : hypothetical protein; Provisional
  • Protein Sequence : Show Sequence 
    >AAA85117.1 M1 protein, partial [Streptococcus pyogenes]
GLRRDLDASREAKKQVEKDLANLTAELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKALEEANSKLA
ALEKLNKELEESKKLTEKAKAELQAKLEAE
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Rivera-Hernandez et al., 2016)
14. M14
  • Gene Name : M14
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : PHP24645
  • Taxonomy ID : 1313
  • Protein Name : peptidase M14
  • Protein pI : 7.55
  • Protein Weight : 18847.03
  • Protein Length : 226
  • Protein Note : genotype: ST-7026
  • Protein Sequence : Show Sequence 
    >PHP24645.1 peptidase M14 [Streptococcus pneumoniae]
MATEWVDVADNAVKIGLGALITIVGGWITLKLTHCHEIRKDAIAQRLRDIDKKTDRYIDFLSSSQTLMQK
YLYESCDPGSEDYTNYMRLHNIVSLTSSPELRIYAFDTQSKVSQFILLRKPNEPEVIDSCRNAARNAVSV
LQGAVSAELLQDKAALQDKQHAWWAFWRK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (McNeil et al., 2005)
15. M18
  • Gene Name : M18
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : WP_011018277
  • Other Database IDs : CDD:291694
    CDD:295219
    CDD:279132
  • Taxonomy ID : 1314
  • Protein Name : LPXTG cell wall anchor domain-containing protein
  • Protein pI : 10.02
  • Protein Weight : 44009.252
  • Protein Length : 497
  • Protein Note : Ciliary rootlet component, centrosome cohesion; pfam15035
  • Protein Sequence : Show Sequence 
    >WP_011018277.1 LPXTG cell wall anchor domain-containing protein [Streptococcus pyogenes]
MVRKDANRQYSLRKLKKSTASVAVALSALGVGLAVNQTEVSAAPLTRATADNKDELIKRANGYEIQNHQL
TVENKKLKIDKEQLTKENDDLKTEKDQLEQRSEKLATQKENLEKEVAEAKHKNETLNINNDDLTKKLNET
RQELANKQQESKENEKTLNELLEKTVKDKIAREQKSKQDFGALKQELAKKEEQNKISEASRKGLRRDLDA
SREAKKQVEKDLANLTAELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKALEEANSKLAALEKLNKE
LEESKKLTEKEKAELQAKLEAEAKALKEQLAKQAEELAKLRAEKASDSQTPDAKPGNKAVPGKGQAPQAG
TKPNQNKAPMKETKRQLPSTGEAANPFFTAAAATVMVSAGMLALKRKEEN
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (McNeil et al., 2005)
16. M22
  • Gene Name : M22
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : AKI36048
  • Other Database IDs : CDD:224135
  • Taxonomy ID : 1314
  • Protein Name : peptidase M22
  • Protein pI : 5.34
  • Protein Weight : 24486.34
  • Protein Length : 288
  • Protein Note : tRNA A37 threonylcarbamoyladenosine modification protein TsaB [Translation, ribosomal structure and biogenesis]; COG1214
  • Protein Sequence : Show Sequence 
    >AKI36048.1 peptidase M22 [Streptococcus pyogenes]
MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAIDFLMTCTDLKPQDLERIVAAKGPGSYT
GLRVAVATAKTLAYSLNIALVGISSLYALAASTCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHA
SLEVIIEQLVEEGQLIFVGETAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRV
EAEENWLKDNEIKDDSHYVKRI
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (McNeil et al., 2005)
17. M28
  • Gene Name : M28
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : OZS35741
  • Taxonomy ID : 1313
  • Protein Name : peptidase M28
  • Protein pI : 5.25
  • Protein Weight : 19618.07
  • Protein Length : 258
  • Protein Note : Derived by automated computational analysis using gene prediction method: Protein Homology.
  • Protein Sequence : Show Sequence 
    >OZS35741.1 peptidase M28, partial [Streptococcus pneumoniae]
VVCAHHDAAHSGKFFEAHIEEVMVELFPGIVERIDTQLPNWWGPILAPALAGVGALRGSRPMMIAGTVGS
ALAAALFADIARSPVVPGANDNLSAVALLVALAERLRERPVKGVRVLLVSLGAEETLQGGIYGFLARHKP
ELDRDRTYFLNFDTIGSPELIMLEGEGPTVMEDYFYRPFRDPVPYTHLTLPT
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (McNeil et al., 2005)
18. M6
  • Gene Name : M6
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : AAA26920
  • Other Database IDs : CDD:273479
    CDD:287215
    CDD:279132
  • Taxonomy ID : 1314
  • Protein Name : M6 mature peptide
  • Protein pI : 9.97
  • Protein Weight : 51620.25
  • Protein Length : 549
  • Protein Note : M6 signal peptide
  • Protein Sequence : Show Sequence 
    >AAA26920.1 M6 precursor peptide [Streptococcus pyogenes]
MAKNNTNRHYSLRKLKKGTASVAVALSVIGAGLVVNTNEVSARVFPRGTVENPDKARELLNKYDVENSML
QANNDKLTTENNNLTDQNKNLTTENKNLTDQNKNLTTENKNLTDQNKNLTTENKELKAEENRLTTENKGL
TKKLSEAEEEAANKERENKEAIGTLKKTLDETVKDKIAKEQESKETIGTLKKTLDETVKDKIAKEQESKE
TIGTLKKTLDETVKDKIAKEQESKETIGTLKKILDETVKDKIAREQKSKQDIGALKQELAKKDEGNKVSE
ASRKGLRRDLDASREAKKQVEKDLANLTAELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKALEEAN
SKLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALKEQLAKQAEELAKLRAGKASDSQTPDAKPGNK
VVPGKGQAPQAGTKPNQNKAPMKETKRQLPSTGETANPFFTAAALTVMATAGVAAVVKRKEEN
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Mansour and Abdelaziz, 2016)
19. maltodextrin-binding proteinSPy1294
  • Gene Name : maltodextrin-binding proteinSPy1294
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes M1 GAS
  • NCBI Protein GI : NP_269421
  • Other Database IDs : CDD:328725
  • Taxonomy ID : 160490
  • Protein Name : putative maltose/maltodextrin-binding protein
  • Protein pI : 8.26
  • Protein Weight : 42253.07
  • Protein Length : 513
  • Protein Note : Type 2 periplasmic binding fold superfamily; cl21456
  • Protein Sequence : Show Sequence 
    >NP_269421.1 putative maltose/maltodextrin-binding protein [Streptococcus pyogenes M1 GAS]
MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLWVPTGSKKSYADTIAKFEKDSGYTVKV
VESEDPKAQEKIKKDASTAADVFSLPHDQLGQLVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYA
FPFGIESQVLFYNKSKLAAEDVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNW
GNEKGAAVLKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYPKVTI
GGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRNIVPANKEVQSSEAVQS
NELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKENDYLTKLQQFDKDIAATK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Lei et al., 2004)
20. mrp
  • Gene Name : mrp
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : ANC26187
  • Other Database IDs : CDD:273479
    CDD:309295
    CDD:330553
    CDD:307064
  • Taxonomy ID : 1314
  • Protein Name : Emm-related protein Mrp
  • Protein pI : 9.54
  • Protein Weight : 45693.37
  • Protein Length : 494
  • Protein Note : Gram-positive signal peptide, YSIRK family; TIGR01168
  • Protein Sequence : Show Sequence 
    >ANC26187.1 Emm-related protein Mrp [Streptococcus pyogenes]
MSKRNPNKHYSLRKLKTGTASVAVALTVLGTGLANTTDVKAETVGRFSDEQVRKAREKAIEDVFDGYTGA
RSVYQSGNLPNRLTPTKLSKLMQQMYKETLQKKEELDTLSKALTHTIEKKIESENAYKKELGQLKAAAEA
EAQKALDALNNKNKQISDLTNENAQLKEAIEGYVQTIQNASREIAAKQQELAAAKSQLEAKNAEIEALKQ
QDASKTEEIAKLQSEAATLENLLGSAKRELTELQAKLDTATAEKAKLESQVTTLENLLGSAKRELTDLQA
KLDAANAEKEKLQSQAAALEKQLEATKKELADLQAKLAATNQEKEKLEAEAKALKEQLAKQAEELAKLKA
DKASGAQKPDTKPGNKEVPTRPSQTRTNTNKAPMAQTKRQLPSTGEETTNPFFTAAALTVIASAGVLALK
RKEEN
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Courtney et al., 2017; Dale et al., 2015)
21. mtsA
  • Gene Name : mtsA
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : NP_268747
  • Other Database IDs : CDD:238557
  • Taxonomy ID : 160490
  • Protein Name : metal binding protein of ABC transporter (lipoprotein)
  • Protein pI : 6.8
  • Protein Weight : 33489.71
  • Protein Length : 416
  • Protein Note : Metal binding protein PsaA. These proteins have been shown to function as initial receptors in ABC transport of Mn2+ and as surface adhesins in some eubacterial species. They belong to the TroA superfamily of periplasmic metal binding proteins that...; cd01137
  • Protein Sequence : Show Sequence 
    >NP_268747.1 metal binding protein of ABC transporter (lipoprotein) [Streptococcus pyogenes M1 GAS]
MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIADMTKAIAGDKIDLHSIVPIGQDPHEY
EPLPEDVEKTSNADVIFYNGINLEDGGQAWFTKLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPH
AWLNLENGIIYSKNIAKQLIAKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFK
YFSKAYGVPSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYSEIFT
DSIAKKGKPGDSYYAMMKWNLDKISEGLAK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Severin et al., 2007)
22. nuclease
  • Gene Name : nuclease
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : ASQ22678
  • Other Database IDs : CDD:225249
  • Taxonomy ID : 1314
  • Protein Name : nuclease
  • Protein pI : 7.01
  • Protein Weight : 92218.78
  • Protein Length : 970
  • Protein Note : genotype: emm75
  • Protein Sequence : Show Sequence 
    >ASQ22678.1 nuclease [Streptococcus pyogenes]
MINKKCIISVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKRESVISDKSNFPVISPYLASVDFGERK
TPLPTPDKGVKVTTEQSIAQVRKGPEERTYTVTGKITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSK
GDIVQLTGTLTRFKGDLQLQQVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNN
TSFLVRDDSGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKKVTSS
NSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDGDLATSDGIRVFAKNAK
VQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVTKTGTAQVPSPLVLGKDRIAPANIIDNDGLR
VFDPEEDAIDYWESMEGMLVAVDDAKILGPMKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFK
KGKQIIKAGDSYKGRLAGPVSYSYGNYKVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFS
ANPSSTKDEKVKRIAESFIRDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGPTYRYVD
IAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSVGRIDPTNAAWKDVRKSLA
AEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQRRHVLANMLAQFAKEGAKHQANIVMLGDFND
FEFTKTIQLIEEGDMVNLVSRHDISDRYSYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRA
SDHDPLLLQLSFPKENDKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Radcliff et al., 2015)
23. oppA
  • Gene Name : oppA
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes M1 476]
  • NCBI Protein GI : BAM31018
  • Other Database IDs : CDD:226636
    CDD:173869
  • Taxonomy ID : 1207470
  • Protein Name : oligopeptide-binding protein
  • Protein pI : 5.2
  • Protein Weight : 68040.6
  • Protein Length : 743
  • Protein Note : ABC-type oligopeptide transport system, periplasmic component [Amino acid transport and metabolism]; COG4166
  • Protein Sequence : Show Sequence 
    >BAM31018.2 oligopeptide-binding protein [Streptococcus pyogenes M1 476]
MTFMKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVNDPKSLDYILTNGGGTTDVITQMV
DGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRDGVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDAL
YVVEDSIKNLKAYQNGEVDFKEVGVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGT
TDPSSILVNGAYFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARLY
PNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNNKDFRQAIQFAFDR
ASFQAQTAGQDAKTKALRNMLVPPTFVTIGESDFGSEVEKEMAKLGDEWKDVNLADAQDGFYNPEKAKAE
FAKAKEALTAEGVTFPVQLDYPVDQANAATVQEAQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAE
TPEQQDYDIISSWWGPDYQDPRTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAIT
DDNDARYKAYAKAQAYLTDNAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVTVKQ
YEKAKEKWMKAKAKSNAKYAEKLADHVEK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Lei et al., 2004)
24. putative protease maturation proteinSpy1390
  • Gene Name : putative protease maturation proteinSpy1390
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes M1 GAS
  • NCBI Protein GI : NP_269488
  • Other Database IDs : CDD:179281
  • Taxonomy ID : 160490
  • Protein Name : putative protease maturation protein
  • Protein pI : 8.27
  • Protein Weight : 36798.43
  • Protein Length : 440
  • Protein Note : foldase protein PrsA; Reviewed; PRK01326
  • Protein Sequence : Show Sequence 
    >NP_269488.1 putative protease maturation protein [Streptococcus pyogenes M1 GAS]
MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSDFYNETKNTEVSQKAMLNLVISRVFEA
QYGDKVSKKEVEKAYHKTAEQYGASFSAALAQSSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKA
YESYTPTMAVEMITLDNEETAKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAAS
SLNEGGISDVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIANALDK
ANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSSAEEPTETEAQTQEPAA
Q
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Lei et al., 2004)
25. putative secreted protein SPy0019
  • Gene Name : putative secreted protein SPy0019
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : NP_268436
  • Other Database IDs : CDD:226400
    CDD:226451
  • Taxonomy ID : 160490
  • Protein Name : putative secreted protein
  • Protein pI : 8.81
  • Protein Weight : 39963.99
  • Protein Length : 476
  • Protein Note : Uncharacterized N-terminal domain of peptidoglycan hydrolase CwlO [Function unknown]; COG3883
  • Protein Sequence : Show Sequence 
    >NP_268436.1 putative secreted protein [Streptococcus pyogenes M1 GAS]
MKKRILSAVLVSGVTLGAATTVGAEDLSTKIAKQDSIISNLTTEQKAAQNQVSALQAQVSSLQSEQDKLT
ARNTELEALSKRFEQEIKALTSQIVARNEKLKNQARSAYKNNETSGYINALLNSKSISDVVNRLVAINRA
VSANAKLLEQQKADKVSLEEKQAANQTAINTIAANMAMAEENQNTLRTQQANLVAATANLALQLASATED
KANLVAQKEAAEKAAAEALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPAALTA
PAAPSAGPQTSYDSSNTYPVGQCTWGAKSLAPWAGNNWGNGGQWAYSAQAAGYRTGSTPMVGAIAVWNDG
GYGHVAVVVEVQSASSIRVMESNYSGRQYIADHRGWFNPTGVTFIYPH
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Bensi et al., 2012)
26. putative surface exclusion protein SPy0269
  • Gene Name : putative surface exclusion protein SPy0269
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : ANC26552
  • Other Database IDs : CDD:330553
    CDD:275124
  • Taxonomy ID : 1314
  • Protein Name : putative surface exclusion protein
  • Protein pI : 7.01
  • Protein Weight : 90520.25
  • Protein Length : 959
  • Protein Note : RecF/RecN/SMC N terminal domain; cl25732
  • Protein Sequence : Show Sequence 
    >ANC26552.1 putative surface exclusion protein [Streptococcus pyogenes]
MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGETKASNTHDDSLPKPETIQEAKATIDAV
EKTLSQQKAELTELATALTKTTAEINNLKEQQDNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTA
TETELHNAQADQHSKETALSEQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDN
TKALSSELEKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKAPQGY
PLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFVDPDNLTPEVQNELAQF
AAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTRPSFVYGQPGVSGHYGVGPHDKTIIEDSA
GASGLIRNDDNMYENIGAFNDVHTVNGIKRGIYDSIKYMLFTDHLHGNTYGHAINFLRVDKRNPNAPVYL
GFSTSNVGSLNEHFVMFPESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAI
HQEADIMAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQSLAKLA
SLKAALHQTEALAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDNTKQDLAKTTSSLLNAQEA
LAALQAKQSSLEATIATTEHQLTLLKTLANEKEYRHLDEDIATVPDLQVAPSLTGVKPLSYSKVETTPLV
QEMVKETKQLLEASARLAAENTSLVAEALVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLI
SDVDESTQRALKAGVVMLAAVGLTGFRFRKESR
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Bensi et al., 2012)
27. putative transcriptional antiterminator (BglG family)SPy1325
  • Gene Name : putative transcriptional antiterminator (BglG family)SPy1325
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes M1 GAS
  • NCBI Protein GI : NP_269442
  • Other Database IDs : CDD:226234
    CDD:224676
    CDD:238129
  • Taxonomy ID : 160490
  • Protein Name : putative transcriptional antiterminator (BglG family)
  • Protein pI : 5.56
  • Protein Weight : 72622.31
  • Protein Length : 774
  • Protein Note : Transcriptional antiterminator [Transcription]; COG3711
  • Protein Sequence : Show Sequence 
    >NP_269442.1 putative transcriptional antiterminator (BglG family) [Streptococcus pyogenes M1 GAS]
MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKELISSLPEHGAHIISKQGRGYCLEIDHS
MAFDIFWQESVTSKKRLADVTQVEESVDREDYVLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKER
IIPYQLELEITHQHIQIRGKEEDIRHFIMDYFFVTSFDNTLSTMVGNTFLEGINFAEIMIIVLDECRDAK
LKLSDFVMNNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEANYIA
LHLKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAHMMPLTIRLENHIQLTN
PLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISLHLMAAIERYSNRHKLRVLVVCATGYGSA
MMLKNRLEKEFEGRLRIVDVISYYEITEERLKTVDLIISSISLANLMFLTPVITVSVFLSNQDIETIRQF
IGEQEGIKKEVSLPSQMSLAKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTETFVEDFYHQ
MVLRENYSPVIYGEVLAFPHPANPMTYSEQVVVVICREPLEWDKAHQAVHFVFLLSPSKGHNHRLKYVSP
GLASFVNQVELQQALLEEPNYSKFMTVFTPLIHD
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Zhang et al., 2006)
28. SCPA
  • Gene Name : SCPA
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : AAT86469
  • Other Database IDs : CDD:234635
  • Taxonomy ID : 286636
  • Protein Name : ScpA
  • Protein pI : 4.8
  • Protein Weight : 26400.86
  • Protein Length : 291
  • Protein Note : segregation and condensation protein A; Reviewed; PRK00104
  • Protein Sequence : Show Sequence 
    >AAT86469.1 ScpA [Streptococcus pyogenes MGAS10394]
MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIETLQVMKLEVAGDYMLMASQLMLIKSRR
LLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVSQALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLF
LAFSNIMAAKRAVLKNNHTVIERDDYKIEDMMASIKQRLEKESVISLSAIFEECQTLNEVISIFLASLEL
IKLHVVFVEQLSNFGAIILRKEKK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Chen et al., 2016)
29. scpA
  • Gene Name : scpA
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : ANC27812
  • Other Database IDs : CDD:173801
    CDD:239048
    CDD:310703
    CDD:328787
    CDD:291893
  • Taxonomy ID : 1314
  • Protein Name : C5a peptidase protein ScpA
  • Protein pI : 5.31
  • Protein Weight : 123577.11
  • Protein Length : 1264
  • Protein Note : Peptidase S8 family domain in Streptococcal C5a peptidases; cd07475
  • Protein Sequence : Show Sequence 
    >ANC27812.1 C5a peptidase protein ScpA [Streptococcus pyogenes]
MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQAVETPQPTAVSEEAPSSSKETNPPQIP
DDAEETVADKANDPAPQAPAKTADTPVTSKATIRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEA
WRLTDKTKARYQSKEDLEKAKKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSET
KEPYRLEGAMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETKKAF
DYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSPDKQLTETATVKTADKQ
DKEMPVLSTNRFEPNKAYDYAYANRGMKEDDFKDVKGKIALIERGDIDFKDKIANAKKAGAVGVLIYDNQ
DKGFPIELPNVDQMPAAFISRKDGLLLKDNPKKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDI
AAPGQDILSSVANNKYAKLSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDE
DEKAYFSPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDTFEVTVTVHNKSDKPQELYYQATVQ
TDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKNGYFLEGFVRFKQDPTKE
ELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDAKDQLDGDGLQFYALKNNFTALTTESNPWT
IIKAVKEGVENIEDIESSEITETIFAGTFAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFL
RNAKNLVAEVLDKEGNVVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRY
TPISSGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDEDLPTTEYI
SPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEGHSNKPEQDGSDQAPDKKPE
TKPEQDGSGQAPDKKPEAKPEQDGSGQTPDKKPEAKPEQDGSGQTPDKKPETKPEKDSSGQTPGKTPQKG
QPSRTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRTED
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Kagawa et al., 2009)
30. scpA
  • Gene Name : scpA
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes M1 GAS
  • NCBI Protein GI : NP_269970
  • Other Database IDs : CDD:173801
    CDD:239048
    CDD:310703
    CDD:328787
    CDD:291893
  • Taxonomy ID : 160490
  • Protein Name : C5A peptidase precursor
  • Protein pI : 5.11
  • Protein Weight : 123854.55
  • Protein Length : 1268
  • Protein Note : Peptidase S8 family domain in Streptococcal C5a peptidases; cd07475
  • Protein Sequence : Show Sequence 
    >NP_269970.1 C5A peptidase precursor [Streptococcus pyogenes M1 GAS]
MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQAVETPQPTAVSEEAPSSKETKTPQTPD
DAEETIADDANDLAPQAPAKTADTPATSKATIRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAW
RLTDKTKARYQSKEDLEKAKKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETK
EPYRLEGAMPEAQLLLMRVEIVNGLADYARNYAQAIIDAVNLGAKVINMSFGNAALAYANLPDETKKAFD
YAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSPDKQLTETATVKTADQQD
KEMPVLSTNRFEPNKAYDYAYANRGMKEDDFKDVKGKIALIERGDIDFKDKIANAKKAGAVGVLIYDNQD
KGFPIELPNVDQMPAAFISRKDGLLLKENPQKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIA
APGQDILSSVANNKYAKLSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDED
EKAYFSPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYYQATVQT
DKVDGKLFALAPKALYETSWQKITIPANSSKQVTIPIDVSQFSKDLLAPMKNGYFLEGFVRFKQDPTKEE
LMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDAKDQLDGDGLQFYALKNNFTALTTESNPWTI
IKAVKEGVENIEDIESSEITETIFAGTFAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLR
NAKNLVAEVLDKEGNVVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYT
PISSGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDEDLPTTEYIS
PNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEGHSNKPEQDGSDQAPDKKPET
KPEQDGSGQAPDKKPETKPEQDGSGQTPDKKPETKPEQDGSGQTPDKKPETKPEKDSSGQTPGKTPQKGQ
PSRTLEKRSSKRALATKASTKDQLPTTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRTED
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Bensi et al., 2012)
31. SfbI
  • Gene Name : SfbI
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes DSM 2071
  • VO ID : VO_0012394
  • NCBI Nucleotide GI : 511149
  • NCBI Protein GI : WP_014407228
  • Protein Accession : CAA48133.1
  • Other Database IDs : CDD:311998
    CDD:331285
    CDD:308566
  • Taxonomy ID : 1314
  • Gene Strand (Orientation) : ?
  • Protein Name : fibronectin-binding protein SfbI
  • Protein pI : 4.15
  • Protein Weight : 64222.46
  • Protein Length : 694
  • Protein Note : Thioester domain; pfam08341
  • DNA Sequence : Show Sequence 
    >gi|511149|emb|X67947.1| S.pyogenes Sfb gene for fibronectin-binding protein (partial)
TAAAGCAATATTTTCTCAAAAAATCATTTTCAAAAACATTAATTTGGTGAAAAATATAATCAAAAAATTG
TCTTTCTTGACAATAACGTGGTAAGCTCATATATGTTTGTGAGAGGAGAGAAAATGAATAACAAAATGTT
TTTGAACAAAGAAGCCGGTTTTTTGGCACACACAAAAAGAAAAAGGCGATTTGCTGTCACTTTAGTGGGA
GTCTTTTTTATGCTTTTGGCATCCGCGGGTGCTATCGGTTTTGGTCAAGTAGCCTATGCTGCCGATGAGA
AGACTGTGCCTCATAGAGTTAGTCAAAATCCTGAGTTTCCCTGGTATGGTTATGATTTCTACAAAGGACC
TTACACAAGATATCATAATTTACAGCTAAATCTCAACGGAAGTAAAACATATCAGGCTTATTGTTTTAAT
TTAAAGAGATTTGAACCTAAGAAAGAAGGTAGTTATTTTCCGAATTGGTATAAGAGGTGGGATGGTAGTG
AAGAGACATTTGTAAAATATGCTGATAACCCCAGAAAAGATAATGAAAGTTCCAGAGTTATAGATGTAGA
ATTAGAAAAAAATATATTAAGAGTGCTATATAATGGATATCCGAATAACGGTAATGGAATCATGGAGGGT
TTAGAACCATTAAATGCTATCTTAGTAACTCAAAATGCTGTTTGGTACTATTCTGATAATTCATCTATTT
TTAATACTGATAACTTCTTTACGACTGAAGCTAAAGATCTTAATATAAAACCTGAGCAATTATCATTAAT
GCGTGTTGCATTAAAGAAATTGATAGATCCTAAGTTATCTGAAGAATCATTGAAACCTGTGCCATCTACT
TTTAGACTGAATATTTTCGAATCTCAGGATAAGCTATATCAAAATCTTCTAAGTGCTGAGTTTGTACCAG
AAAATCCCCCTAAACCTGGTGAAACCCCTGAACATGGACCCAAAACTCCCGAGTTGGATGGCACTCCAAT
TCCCGAAGGCCCACAACGTCCAAATGAGAGTTTAGAACCTACGCTTCCACCAGTGATGCTAGACGGACAA
GAAGTCCCCGAAGTTCCAAGTGAGAGCTTAGAACCTGCGCTTCCCCCATTGATGCCAGAGCTAGATGGCC
AAGAAGTCCCAGAAGTTCCAAGCGAGAGCTTAGAACCTGCGCTTCCCCCATTGATGCCAGAGTTAGATGG
TCAAGAAGTCCCTGAAAAACCTAGTGTTGACTTACCTATTGAAGATCCTCGTTATGAGTTTAACAATAAA
GACCAATCACCTCTAGCGGGTGAGTCTGGTGAGACGGAGTATATTACCGAAGTTTATGGAAATCAACAGA
ACCCTGTTGATATTGATAAAAAACTTCCGAATGAAACAGGTTTTTCAGGAAATATGGTTGAGACAGAAGA
TACGAAAGAGCCAGGAGTGTTGATGGGAGGTCAAAGTGAGTCTGTTGAATTTACTAAAGACACTCAAACA
GGCATGAGTGGTCAAACAACTCCTCAGGTTGAGACAGAAGACACGAAAGAGCCAGGAGTGCTGATGGGAG
GTCAAAGTGAGTCTGTTGAATTTACCAAAGATACTCAAACAGGCATGAGTGGTCAAACAGCTTCTCAGGT
TGAGACAGAAGATACGAAAGAGCCAGGAGTGCTGATGGGAGGCCAAAGTGAGTCTGTTGAATTTACTAAA
GACACTCAAACAGGCATGAGTGGTCAAACAACTCCTCAGGTTGAGACAGAAGACACGAAAGAGCCAGGAG
TATTGATGGGAGGTCAAAGTGAATCTGTTGAATTTACTAAAGACACTCAAACAGGCATGAGCGGTTTCAG
TGAAACAGTGACCATTGTTGAAGATACCCGTCCGAAGTTAGTGTTCCATTTTGACAATAATGAGCCCAAA
GTGGAAGAGAATCGGGAAAAGCCTACAAAAAATATAACACCTATCCTTCCTGCAACAGGAGATATTGAGA
ATGTTTTGGCCTTTCTTGGAATCCTTATTTTGTCAGTACTTCCTATTTTTAGTCTTTTAAAAAAACAAAC
AAAACAATAAAGTCTGATCGTAAAAGTGTTCCATAAGAAATAATGCGCAAATATGCTGAATAGTGGATAA
CTGCTACAGTCAAGACAAGTGTTAATTCAAAAGGTACTACTTATACATCTGATAATAATAAGGTGACAGA
TAATTCAGAGCAAGTAGGATGGCTAAAAAATGGTGTATTCTATGTATAATAGACTACATAGAAATCAAGT
TATTGTTAAACGTCGTAAAGCTT
  • Protein Sequence : Show Sequence 
    >WP_014407228.1 fibronectin-binding protein SfbI [Streptococcus pyogenes]
MNNKIFLNKEAGFLAHTKRKRRFAVTLVGVFFMLLACAGAIGFGQVAYAADERTVPNHSSTNPEFPWYGY
DSYRGIFARYHNLKVNLKGSKEYQAYCFNLTKYFPRPTYSTTNNFYKKIDGSGSAFKSYAANPRVLDENL
DKLEKNILNVIYNGYKSNANGFMNGIEDLNAILVTQNAIWYYSDSAPLNDVNKMWEREVRNGEISESQVT
LMREALKKLIDPNLEATAVNKIPSGYRLNIFKSENEDYQNLLSAEYVPDDPPKPGDTSEHNPKTPELDGS
PIPEDPKHPDDNLEPTLPPVMLDGEEVPEVPSESLEPALPPLMPELDGEEVPEVPSESLEPALPPLMPEL
DGEEVPEVPSESLEPALPSLMPELDGEEVPEVPSESLEPAFPPLMPELDGQEVPEKPSVDLPIEVPRYEF
NNKDQSPLAGESGETEYITEVYGNQQNPVDIDKKLPNETGFSGNMVETEDTKEPEVLMGGQSESVEFTKD
TQTGMSGQTTPQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGFSETVTIVEDTRPKLVFHFDNNEPKV
EENREKPTKNIAPILPATGDIGNVLAFLGILILSVLSIFSLLKNKQNNKV
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Intranasal immunization of mice with either SfbI alone or coupled to cholera toxin B subunit (CTB) triggered efficient SfbI-specific humoral (mainly IgG) and lung mucosal (14% of total IgA) responses. SfbI-vaccinated animals showed 80% and 90% protection against homologous and heterologous challenge, respectively (Guzmán et al., 1999).
    Peptides incorporated in the Lipid-Core Peptide (LCP) vaccine delivery system derived from the important streptococcal virulence factors M protein and the fibronectin-binding protein SfbI. The present work clearly demonstrates the potential of these synthetic dual antigen multi-epitope mucosal GAS prophylactic vaccines as an approach to deliver vaccine antigens and adjuvants across mucosal membranes resulting in the stimulation of protective immune responses.(Schulze et al., 2017)
  • Related Vaccine(s): S. pyogenes SfbI Protein Vaccine
32. shp
  • Gene Name : shp
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : ANC27645
  • Other Database IDs : CDD:288407
  • Taxonomy ID : 1314
  • Protein Name : heme binding protein Shp
  • Protein pI : 8.57
  • Protein Weight : 30966.08
  • Protein Length : 359
  • Protein Note : Cell surface heme-binding protein Shp; pfam11545
  • Protein Sequence : Show Sequence 
    >ANC27645.1 heme binding protein Shp [Streptococcus pyogenes]
MTKVVIKQLLQVIVVFMISLSTMTNLVYADKGQIYGCIIQRNYRHPISGQIEDSGGEHSFDIGQGMVEGT
VYSDAMLEVSDAGKIVLTFRMSLADYSGNYQFWIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTV
NSIIRGSMFVEPMGREVVFYLSASELIQKYSGNMLAQLVTETDNSQNQEVKDSQKPVDTKLGESQDEPHT
GAMITQNKPKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGFAVWV
WKKRKKNDKTM
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Zhang et al., 2017)
33. sib35
  • Gene Name : sib35
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes serotype M12
  • VO ID : VO_0012398
  • NCBI Protein GI : 90567992
  • Other Database IDs : CDD:286161
  • Taxonomy ID : 342023
  • Gene Strand (Orientation) : ?
  • Protein Name : immunoglobulin-binding protein 35
  • Protein pI : 7.68
  • Protein Weight : 36275.97
  • Protein Length : 431
  • Protein Note : Mac 1; pfam09028
  • Protein Sequence : Show Sequence 
    >BAE92207.1 immunoglobulin-binding protein 35 [Streptococcus pyogenes serotype M12]
MRKRCYSTSAAVLAAVTLFVLSVDRGVIADSFSANQEIRYSEVTPYHVTSVWTKGVTPPAKFTQGEDVFH
APYVAKQGWYDITKTFNGKDDLLCGAATAGNMLHWWFDQNNEKIEAYLKKHPDKQKIMFGDQELLDVRKV
INTKGDQTNSELFNYFRDKAFPGLSARRIGVMPDLVLDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIF
DAVFTRGDQSKLLTSRHDFKEKTLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSNGNLE
AIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQDSWNQTN
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Immunization with Sib 35 enhanced survival rates in mice challenged with a GAS strain, while exhibiting no toxicity in hosts (Okamoto et al., 2005).
  • Related Vaccine(s): S. pyogenes Sib35 Protein Vaccine
34. slo
  • Gene Name : slo
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : NP_268546
  • Other Database IDs : CDD:307453
  • Taxonomy ID : 160490
  • Gene Strand (Orientation) : ?
  • Protein Name : streptolysin O precursor
  • Protein pI : 8.23
  • Protein Weight : 60115.22
  • Protein Length : 651
  • Protein Note : Thiol-activated cytolysin; pfam01289
  • Protein Sequence : Show Sequence 
    >NP_268546.1 streptolysin O precursor [Streptococcus pyogenes M1 GAS]
MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETTTTNEQPKPESSELTTEKAGQKTDDML
NSNDMIKLAPKEMPLESAEKEEKKSEDKKKSEEDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGV
KKADKFIVIERKKKNINTTPVDISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMG
DKATVEVNDPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDGTLGI
DFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKELQRKGVSNEAPPLFVSNVAYGRTVFV
KLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAVVLGGDAAEHNKVVTKDFDVIRNVIKDNA
TFSRKNPAYPISYTSVFLKNNKIAGVNNRTEYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKG
KEVITKRRWDNNWYSKTSPFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGS
TLSPYGSITYK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Bensi et al., 2012)
35. spa
  • Gene Name : spa
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : AIG88580
  • Other Database IDs : CDD:288951
    CDD:287215
  • Taxonomy ID : 1314
  • Protein Name : Spa
  • Protein pI : 4.97
  • Protein Weight : 55020.08
  • Protein Length : 605
  • Protein Note : RNA polymerase recycling family C-terminal; pfam12137
  • Protein Sequence : Show Sequence 
    >AIG88580.1 Spa, partial [Streptococcus pyogenes]
TRIAQAVTAAVLMGTAVTTVGGALGTTTTVKAYQINQDDLSKLTEAIVNKEMIRHGGGKSQSDIIKDVKS
ILSEAKVETLQTLLQGFDSTSPGYAEGLTYLGNLTNRLGSFNTGLDGASSAEAYHGYLTDLRRMVVEEVQ
SRVASEAELKTKLAEKEKVAERLGEELTKVIKLKSDIELNAKKAQGELTKTKQQLEEALAKSEELSTDLL
SAQKERDTAFKVSKELADKLAESEKGKANALKVSKELADKLATAESGRDKALAISAELANKLAAKTAEAE
ELLNNVDSFGRLVESAKREMQEKLAEIEALKAEKAQADAALETANATIAGLQAEVEKVKSDLANANQLVE
AGKRDLADLAKAKAEADAALATSEAKVAKLEKAKAEADAKVTELEAAVTAAKEEVAKVKAELETVTKELE
TVKAEKAALEAEITDLKKQHAEKVAELEAAIKRLEEELAAKIKEFEALENTSKEEKANFQKEIDRLKAEL
AAKIKDLTKAGSSVATGTASTGVASSKAQLPSTGEATHPFFTAAALAVMAGAGVMA
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (McNeil et al., 2005)
36. spa
  • Gene Name : spa
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : ACD81471
  • Other Database IDs : CDD:309682
    CDD:331876
    CDD:330553
    CDD:307064
  • Taxonomy ID : 1314
  • Protein Name : streptococcal protective antigen
  • Protein pI : 6.3
  • Protein Weight : 58450.81
  • Protein Length : 632
  • Protein Note : YSIRK type signal peptide; pfam04650
  • Protein Sequence : Show Sequence 
    >ACD81471.1 streptococcal protective antigen [Streptococcus pyogenes]
MFLRNKKQKFSIRKLSAGAASVLVAASVLGGAVVNVHAEDRINSDINRRIVDKKDAENLRNAIIAKGARL
KNDSEVISFLREALSNATVDTLATLLSGIDPARFGYSNSQLEFYSRQLGSLNSGVSDWQQGSVNLKTLLI
EEFAKRIKSEQKLKEVLAEQAAELELRKSEESELKTQKERLESKLENAEYATAIKQKELDDAKEANKTLS
ESIAKTLSRSTKEKDKLKEELVKEKTKAAKIAKELMDKLTASEKDKDRAIQITTELTNRLHETEASRDKA
FAVSTELANKLAAKTAEAEKLMQNVGSLDRLVESAKREIAEKLAEIDQLTADKAKADAELATANDTIASL
QTELEKAKTELAVSERLIESGKREMAELQKQKDASDKALAESQANVAELEKQKAASDAKVAELEKEVEAA
KAEVADLKAQLAKKEEELEAVKKEKEALEAKIEELKKAHAEELSKLKEMLEKKDHANADLQAEINRLKQE
LADRIKSLSQGGRASQANPGSTTAKAGQLPSTGESANPFFTIAALTVIAGAGMAVVSPKRKEN
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Niedermeyer et al., 2014)
37. speA
  • Gene Name : speA
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : WP_011285560
  • Other Database IDs : CDD:279467
    CDD:280949
  • Taxonomy ID : 1314
  • Gene Strand (Orientation) : ?
  • Protein Name : streptococcal pyrogenic exotoxin SpeA
  • Protein pI : 5.97
  • Protein Weight : 27919.46
  • Protein Length : 335
  • Protein Note : Staphylococcal/Streptococcal toxin, OB-fold domain; pfam01123
  • Protein Sequence : Show Sequence 
    >WP_011285560.1 streptococcal pyrogenic exotoxin SpeA [Streptococcus pyogenes]
MENNKKVLKKMVFFVLVTFLGLTISQEVFAQQDPDPSQLHRSSLVKNLQNIYFLYEGDPVTHENVKSVDQ
LLSHDLIYNVSGPNYDKLKTELKNQEMATLFKDKNVDIYSVEYYHLCYLCENAERSACIYGGVTNHEGNH
LEIPKKIVVKVSIDGIQSLSFDIETNKKMVTAQELDYKVRKYLTDNKQLYTNGPSKYETGYIKFIPKNKE
SFWFDFFPEPEFTQSKYLMIYKDNETLDSNTSQIEVYLTTK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Morefield et al., 2014)
38. speB
  • Gene Name : speB
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : ANC27833
  • Other Database IDs : CDD:316273
    CDD:307663
  • Taxonomy ID : 1314
  • Protein Name : strepotococcal cysteine protease (streptopain) protein SpeB
  • Protein pI : 8.97
  • Protein Weight : 40955.34
  • Protein Length : 502
  • Protein Note : Spi protease inhibitor; pfam13734
  • Protein Sequence : Show Sequence 
    >ANC27833.1 strepotococcal cysteine protease (streptopain) protein SpeB [Streptococcus pyogenes]
MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAITFIQKSAAIKAGARSAEDIKLDKVNLG
GELSGSNMYVYNISTGGFVIVSGDKRSPEILGYSTSGSFDVNGKENIASFMESYVEQIKENKKLDTTYAG
TAEIKQPVVKSLLDSKGIHYNQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKG
LKDYTYTLSSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDYGPSS
GSAGSSRVQRALKENFGYNQSVHQINRGDFSKQDWEAQIDKELSQNQPVYYQGVGKVGGHAFVIDGADGR
NFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSAVVGIKP
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Tsao et al., 2013)
39. Spy0416
  • Gene Name : Spy0416
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : NP_268723
  • Other Database IDs : CDD:309682
    CDD:173801
    CDD:239048
    CDD:310703
    CDD:273478
  • Taxonomy ID : 160490
  • Gene Strand (Orientation) : ?
  • Protein Name : putative cell envelope proteinase
  • Protein pI : 6.53
  • Protein Weight : 170025.7
  • Protein Length : 1751
  • Protein Note : YSIRK type signal peptide; pfam04650
  • Protein Sequence : Show Sequence 
    >NP_268723.1 putative cell envelope proteinase [Streptococcus pyogenes M1 GAS]
MEKKQRFSLRKYKSGTFSVLIGSVFLVMTTTVAADELSTMSEPTITNHAQQQAQHLTNTELSSAESKSQD
TSQITLKTNREKEQSQDLVSEPTTTELADTDAASMANTGSDATQKSASLPPVNTDVHDWVKTKGAWDKGY
KGQGKVVAVIDTGIDPAHQSMRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIK
ENQFEDFDEDWENFEFDAEAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDDTKYESHG
MHVTGIVAGNSKEAAATGERFLGIAPEAQVMFMRVFANDIMGSAESLFIKAIEDAVALGADVINLSLGTA
NGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDPLATNPDYGLVGSPSTGRTPTSVAAINSK
WVIQRLMTVKELENRADLNHGKAIYSESVDFKDIKDSLGYDKSHQFAYVKESTDAGYNAQDVKGKIALIE
RDPNKTYDEMIALAKKHGALGVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLE
FDSVVSKAPSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIAGASLLV
KQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNIDGAVTSGLYVTGKDNYGS
ISLGNITDTMTFDVTVHNLSNKDKTLRYDTELLTDHVDPQKGRFTLTSHSLKTYQGGEVTVPANGKVTVR
VTMDVSQFTKELTKQMPNGYYLEGFVRFRDSQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTG
FYFDESGPKDDIYVGKHFTGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPN
GDNNQDFAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTLLGTAFSGK
SLTGAELPDGHYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDPETNRFKPEPLKDRGLAGVRK
DSVFYLERKDNKPYTVTINDSYKYVSVEDNKTFVERQADGSFILPLDKAKLGDFYYMVEDFAGNVAIAKL
GDHLPQTLGKTPIKLKLTDGNYQTKETLKDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFIS
PNEDGNKDFVAFKGLKNNVYNDLTVNVYAKDDHQKQTPIWSSQAGASVSAIESTAWYGITARGSKVMPGD
YQYVVTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALDSSGIVREEVFYLAKKNG
RKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVEDRAGNVSFATLRDLKAVGKDKAV
VNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYYNNSGNSLILPYGKYTVELLTYDTNAAKLESDKIV
SFTLSADNNFQQVTFKITMLATSQITAHFDHLLPEGSRVSLKTAQDQLIPLEQSLYVPKAYGKTVQEGTY
EVVVSLPKGYRIEGNTKVNTLPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTASATPTKSTTSAT
AKALPSTGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Pandey et al., 2015)
    Both the hypothetical protein SPy0843 and the putative cell envelope proteinase (SPy0416) have been shown to be protective in a mouse challenge model following active immunization with the respective protein(Severin et al., 2007)
40. Spy0469
  • Gene Name : Spy0469
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : NP_268760
  • Other Database IDs : CDD:197609
    CDD:331207
  • Taxonomy ID : 160490
  • Gene Strand (Orientation) : ?
  • Protein Name : putative 42 kDa protein
  • Protein pI : 4.31
  • Protein Weight : 38867.52
  • Protein Length : 465
  • Protein Note : Lysin motif; smart00257
  • Protein Sequence : Show Sequence 
    >NP_268760.1 putative 42 kDa protein [Streptococcus pyogenes M1 GAS]
MIITKKSLFVTSVALSLVPLATAQAQEWTPRSVTEIKSELVLVDNVFTYTVKYGDTLSTIAEAMGIDVHV
LGDINHIANIDLIFPDTILTANYNQHGQATNLTVQAPASSPASVSHVPSSEPLPQASATSQPTVPMAPPA
TPSDVPTTPFASAKPDSSVTASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISE
APTSANRPVPNESASEEVSSAAPAQAPAEKEETSAPAAQKAVADTTSVATSNGLSYAPNHAYNPMNAGLQ
PQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPENSALGDQVAQYAIDHMAERGISYVIWKQ
RFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFNA
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Mortensen et al., 2016)
41. srtA
  • Gene Name : srtA
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : BAB08162
  • Taxonomy ID : 1314
  • Protein Name : SrtA
  • Protein pI : 8.9
  • Protein Weight : 5237.51
  • Protein Length : 90
  • Protein Note : latibiotics precursor
  • Protein Sequence : Show Sequence 
    >BAB08162.1 SrtA [Streptococcus pyogenes]
MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTTVK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Chen et al., 2016)
42. StreptInCor
  • Gene Name : StreptInCor
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • Taxonomy ID : 32630
  • Protein Name : StreptInCor
  • Protein pI : 10.55
  • Protein Weight : 7012.252
  • Protein Length : 101
  • Protein Note : helix 1
  • Protein Sequence : Show Sequence 
    >2KK9_A StreptInCor 55 amino acid residues of the C-terminal portion of the M protein
KGLRRDLDASREAKKQLEAEQQKLEEQNKISEASRKGLRRDLDASREAKKQVEK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Guerino et al., 2011)
43. tmbC
  • Gene Name : tmbC
  • Sequence Strain (Species/Organism) : Streptococcus pyogenes
  • NCBI Protein GI : BAQ53101
  • Other Database IDs : CDD:224658
  • Taxonomy ID : 1314
  • Protein Name : basic membrane family protein
  • Protein pI : 8.3
  • Protein Weight : 33607.07
  • Protein Length : 423
  • Protein Note : Basic membrane lipoprotein Med, periplasmic binding protein (PBP1-ABC) superfamily [Cell wall/membrane/envelope biogenesis]; COG1744
  • Protein Sequence : Show Sequence 
    >BAQ53101.1 basic membrane family protein [Streptococcus pyogenes]
MNKKFIGLGLASVAVLSLAACGNRGASKGGASGKTDLKVAMVTDTGGVDDKSFNQSAWEGLQSWGKEMGL
QKGTGFDYFQSTSESEYATNLDTAVSGGYQLIYGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVAS
VTFADHEAAYLAGIAAAKTTKTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKG
KTIAAAQYAAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEANFVL
ASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIKEAKAKIKSGDIKVPEK
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Lei et al., 2004)
1. IgA
  • Gene Name : IgA
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 238447
  • Genbank Accession : AC160982
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 8607
  • Gene Ending Position : 12639
  • Gene Strand (Orientation) : -
  • Protein Name : immunoglobulin heavy constant alpha
  • Protein Note : Also known as IgA; Igh-2
  • DNA Sequence : Show Sequence 
    >gi|121699722:8607-12639 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
AGTACTGGGGGACCTCTTTGCTGCCAAACGGGCCTCGAACAGTTGTAACAGTGAGTGCTGTGCTGTAGAA
CAAGCTCAGTAGGAAGAGGGTGAGGAAGGTCACAGTGGTGGGCCACAGGCTGGCACCTGGGGCCTCTTCC
TCCAGGATGTCTTCTGACTGGTCCAGTAGCACATAGGAAAGTGGCTCTTGACGTTCTGTAAGACATAGAT
GACCTTCTAGATCCAAACCCATGGGGATGGGGGCTCAGACTTAAGGTTTCTGCTTCTATGCCTCCTTGGG
CTTTGGGGACAGTGGTGGTAGGGGTCCCTTATTCCCGGGAAGGGACTTGTTCAGGGATAATGAGGAGGCA
GGGTCCCAGGTAAGGGACTGGAATCCCAGCAGTTCTTGAGATGGCCCCAAGACCCATCTCTCTCTCTTAG
GGAAACCTTAATTAGAGACCTGTCATGGTAAGAGAGTTGTTTCTGTCAAACTCTGAGAAGAGTTTGAACC
CAAGGCTCCTTAGGTCTCAGTCCTTTCTAGATATTTAGCAATACCTCCCTAAACCCCAGTTCTTAGGCTA
ATCTTGGCTTGGGTACTAGGGTTGTGTAAGATCAGACACCTTCAAACAGATACAGGCCAATGTGATTTCT
GGGCCAAAGTTAGACTACCAAGGCCTGGACATGTGGTCAGAGGACTCTGTTGCATTCTTAAGAGCTGTGA
CTGGGGGTTAAGCAACAGTAAAGACCCCCAGTAGAGCCAACCCTGCTGTCCTCAGGTTCTCTGCGCACAG
AGTTTCATTTTAAGTGGCAGTAGGAAATGTGTACTGATAGTCAACCACTCGTATTTCTGTCACTGCAGCC
ATCCATCAATCCATCCATCATCCCCATCGTTCATCCTTGTAGCCAGCCATCCATTACCCTCCAGCCGCTC
ACTCATCATCTTTCCCGACTATCGTGATTCTCCAGCTTCTCTGCCCCCTCTCCCTGTGGGGATTCAGTCT
TTCACCTATTGACTCCTCCTTACTAGGATTCTCTGTCATGGGCCAGGCCTTGGATTCCATGGGTGGCTAT
GGTACTTTTCTGAGTCAGGTTTCCCAAGCTGGTCTGTTGCTCTTGCTATTCTCAGGGAACTGGACTTTCA
AGCTGGAGAGTATCCAGGGCATGCATGGGCCTTTACTCCACTCTTGCTCTGAGAGCAGAGGGAAGTTGCT
AGAAACCAGCAACTCCTCCCCCCCCCCCCCGAAGTTCTGAAGGCCTGTGAGACAGGAGCAGGGCCCCCAC
TGCCCTCTTGTGGACACCTGGGCTCCAAGTATACCTGGTTGTAGAACTTTGTGTTCTAGGGGTGCCCATG
GAGGAGGCCGAGGCCTGTGTGGGAGTGGTCTCCATGGCCTGCTCCTGCTTCTTCCCAGATTTGCCTCTTA
TCCTGCTTCTGGTTTGTGATACTTGGGCTCAGGATGGGAAGGGAGACTTAGGTTAGAAAGGGGGCACCTG
GAGGGGCCTATGCCCTTGTCCATTGTGTGTAGTTGTCAGTGTCGGGCCTGGAAATTCTCCCGGGGGCTTG
GAGGGGACTCCCCTTGCTTTCCAGAAGCTGGGTGTGGGCTGTGAACTTCATCTTCCTCCAGGGTTCAGGT
CTGTGTGAGTGTGCAAATGTGAGTGTCTGTGCATGTATGCCCATGTGCATGTATGCAGAACAGGGTGTAT
GCCCATGTGCATGTGTGAAGAGCAGGGTGCTGCTGAGGGTTGTCTGTGCCATGCTATATGTCTTTCTCAT
GAGTCAGTGGTCACCCTGAATGCATGTTTCCATTCAGGGATGGCCTACTGAGGGTGCGTGGCATCTTCTT
CCCAGTGCCCCTGTGTTCATCCCTTCATTCTCATACCATCATCTCTATTGTGACCCCCACACGGCACCAG
CCTTGATGGTGCTACCCTTTTGCTCCATCTATCCCTGTCTTGTTTGGGTTAGAAGCTGCGGTCTAATAAA
CAGCCACACCACCCACCACTCAGAGTACTTGTGCAGCAACCCACTGCCCTGGGGACTCTCCCATGAGCTT
TGCCTTCTTGGCTGAGTCTGCTCGCCTTGGATTTCATCCCCAGTTTATTCTTACTTCCCATTCACTCATT
GCACATTCACAGGGCAGGTGCACACACTCTCATGTTTCCACCAACATGAACACACATAGATATATGCATG
CTCATAGGTATGCACAGGCACACACTTGCTCACAGGGACATAGATATAGTGTGTCCACATATGTACTTAT
ACCCATATACATACCTATAAACCATAGGTGCACACAAACTCACTCCTCTCTTGCTGCACACAGGTACACA
CATGAAGGCACATGGACCCATCCATAAGCACTCACCAATACATAGACATGCATACACATATACTCTAGGC
ATGAAGGGTCTTCAAAGTCTCCGAGGCCTTACAAGCTTAGGTGGTGGTGTTTCTCCCTCCCTGGGCCCTG
ACCCCTCCCTGTGTTCTGTAGACCCTAAGGATGATGGACAGGCACTGGATGGAAGTGCAGGGATACTTTG
GATGAGCACAGAGTTTATTTCAGGAGTAGGGACAGGCAGGGTGGCTCAGTAGCAGATGCCATCTCCCTCT
GACATGATCACAGACACGCTGACATTGGTGGGTTTACCCGACAGACGGTCGATGGTCTTCTGGGTGAAGT
TCATGGGCAAGGCCTCGTGGCCCACCATGCAGGAGTACTGGTCACCCTGTTTCCAGAGTTCAGCTGATAC
ACGCAACACGCTTGTCACCAGGTAGGTGGTGGCTCCCTCGCCTGGCTCCTTTAGGGGCTCAAACACTAGG
TAGCTTTCTGGGGACAGCTCCTCATTTCCATGCAGCCATCGCACCAGCACTTCTTTAGGGTTGAAAGCTC
GCACCAGGCATGTCAGGGACACGAGCTCATTCAGGGCCAGCTCCTCCGACGGCGGCGGTAGCAGGTGGAC
CTGGGGTGGGAAGGTGTTCACTGGAAGGTAGAAAGAAGAGTTATGGCTCAGACAAGGAGAGCAAGACCCC
TCTGCCCTTCCCTTCTGGGCAGTTCCTCAGATAGCTCCAATTTCCCTCTGTGATATAAGGACAGGAGCAG
TTAGTTGAGCATCTGTATTATAGGAAGAAAGCAGGCATGTGAGCAGGGAACGTCATACAATGTCCCGGGT
ATGCATCTGGGCTCACCTGTGATTTTGGCAATTGTGCCAGTTAAGGTGTCAGACTCAGGATGGGTAACTG
TGCACTTGAATGATGCGCCACTGTTCCAGCGCTCAGCACAGCCAGGCAGGACGCTGGACACACTGTAGCA
GCCGCAGGAATTCTGCACAGCTTTCTTCTGCACTGCATCCTTCCCAGTGGAGGGCTCCCAGGTGAAGACA
GCTCCCTCAGGATTTCTCAGGCCATTCAGAGTACATGTGAGGCTGGCATCTGAACCCAGGAGCAGGTCCT
CAAGAGCTGGCCGCTGCAGTGACAGGCTGGGATGGCAGGAAGGAGGACAAGGAGGACAAGGAGGAGGAGG
ACCTGTAACAGAGAAGTCCTAGCAAATCAGTATATTCTCCTTTTCTCCTTTGCTGTCCCCTTTTCTGGAT
GTTCTGAGGCCTCAGTTCCTTATGGCCTGTTTACGCTTCCCTTCCCCCACCCCCATGGGAGGTTTCTGAG
GGTCCCTGTGAGGGTGTGCCTCAGAGGGATATATGGATCTAGATAAGGTAGAACTTATCCCACCCCCAGC
TGACCCCCTAACGTTCTTTACCAGAGCACTTCACATCCAATTCTTGGACGGCGTTAGAGTCATGTTGCAC
GGAACATTTCACGGATTCTCCTTCTGGGCACTCGACAGCTGGCAGGGTCAACTGGCTGCTCATGGTGTAC
CCTCCCCCAGAGGCCAGGGCAGGTGGGAAGTTTACGGTGGTTATATCCTTCCCACTCTTTCCCCAGGTCA
CATTCATCGTGCCGGAAGGGAAGTAATCGTGAATCAGGCAGCCGATTATCACTGGGTCACTTGACAGAGC
TCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGAC
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): S. pyogenes FBP54 Protein Vaccine , S. pyogenes SfbI Protein Vaccine
2. IgG
  • Gene Name : IgG
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16059
  • Genbank Accession : AF010213
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 881411
  • Gene Ending Position : 914690
  • Gene Strand (Orientation) : +
  • Protein Name : immunoglobulin heavy chain (V7183 family)
  • Protein Note : Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)
  • DNA Sequence : Show Sequence 
    >gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
ACAAGCACAGTGCAAATTTCTGTCACCCTG
  • Molecule Role : Vaximmutor
  • Additional Molecule Role : Vaximmutor
  • Related Vaccine(s): S. pyogenes FbaA Protein Vaccine , S. pyogenes FBP54 Protein Vaccine , S. pyogenes SfbI Protein Vaccine , S. pyogenes Sib35 Protein Vaccine
IV. Vaccine Information
1. Group A streptococcal recombinant fusion peptide Vaccine
a. Vaccine Ontology ID:
VO_0004247
b. Type:
Subunit vaccine
c. Status:
Clinical trial
d. Adjuvant:
e. Immunization Route
Intramuscular injection (i.m.)
f. Description
A recombinant fusion peptide group A streptococcal vaccine containing N-terminal M protein fragments from serotypes 1, 3, 5, 6, 19, and 24 (Kotloff et al., 2004).
g. Human Response
  • Vaccination Protocol: Twenty-eight healthy adults aged 18 to 50 years were vaccinated. Groups of volunteers received 3 spaced intramuscular injections of either 50-µg (n = 8), 100-µg (n = 10), or 200-µg (n = 10) doses of vaccine into the deltoid muscle of alternating arms. Escalation to a higher dose level required approval by an independent data and safety monitoring committee that reviewed all data from recipients of the lower dose group collected between day 0 until 14 days after the third inoculation. A day 0, 28, and 56 dosing schedule was chosen initially. When 1 of 8 recipients of a 50-µg dose experienced moderate induration and pruritus at the injection site following the third injection, the sponsor and independent data and safety monitoring committee recommended that the dosing schedule for subsequent groups be changed to 0, 28, and 112 days (Kotloff et al., 2004).
  • Immune Response: Vaccination elicited 4-fold or more increases in 40 (83%) of 48 possible assays (ie, 6 antigens × 8 volunteers) following the 50-µg dose, in 45 (75%) of 60 possible assays (ie, 6 antigens × 10 volunteers) following the 100-µg dose, and in 57 (95%) of 60 possible assays (ie, 6 antigens × 10 volunteers) following the 200-µg dose. The geometric mean antibody titer following vaccination was significantly higher than the prevaccination titer for each antigen measured in all dose groups (Kotloff et al., 2004).
  • Side Effects: Vaccination was well-tolerated. Local erythema, swelling, or tenderness were detected in 6 (21%) of 29 participants within 7 days after the first inoculation and 12 (43%) of 28 participants each after the second and third inoculations. All local reactions were graded as mild (≤30 mm and easily tolerated) with the exception of the aforementioned participant who developed moderate (31-80 mm) erythema, swelling, and itching for 1 day after the third dose of 50 µg (Kotloff et al., 2004).
2. S. pyogenes FbaA Protein Vaccine
a. Vaccine Ontology ID:
VO_0004054
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Purified FbaA protein
e. Gene Engineering of fbaA
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant:
g. Immunization Route
subcutaneous injection
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunized subcutaneously with purified FbaA + Freund’s adjuvant at weeks 0, 2, and 4 (Ma et al., 2009).
  • Challenge Protocol: Mice immunized with FbaA or PBS were injected i.p. with 1 ml bacterial suspension (equivalent to 2 × 10^8 CFU) and monitored
    survival daily for 15 days (Ma et al., 2009).
  • Efficacy: 60% of mice immunized with FbpA were protected from challenge with group A streptococcus (Ma et al., 2009).
  • Host Gene Response of IgG
    • Gene Response: Assay for immune response showed that FbaA could induce protein-specific high serum IgG titer in BALB/c mice 10 days after injection. There was no significant difference between the antibody titers of the FbaA vaccine and an M protein vaccine, which is the best immugen of group A streptococcus, and FbaA induced significantly higher titers than PBS-vaccinated mice (Ma et al., 2009).
    • Detailed Gene Information: Click here.
3. S. pyogenes FBP54 Protein Vaccine
a. Vaccine Ontology ID:
VO_0004055
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Recombinant FBP54 protein
e. Gene Engineering of fbp54
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant:
g. Immunization Route
Orally
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were orally immunized with cholera toxin (CT; 10 μg)-FBP54 (10 μg) or CT (10 μg)-PBS by using an intubation needle. Immunization was performed four times, on days 0, 14, 28, and 42 (Kawabata et al., 2001).
  • Challenge Protocol: Actively immunized mice were intraperitoneally challenged with an appropriate number of CFU of S. pyogenes SSI-1 (M3), SSI-9 (M1), or S42 (M12) on day 51 following immunization (Kawabata et al., 2001).
  • Efficacy: Mice subcutaneously or orally immunized with FBP54 survived significantly longer following the challenge with S. pyogenes than did nonimmunized mice (Kawabata et al., 2001).
  • Host Gene Response of IgA
    • Gene Response: An enzyme-linked immunosorbent assay revealed that antigen-specific IgG antibodies were induced in the sera of immunized mice, while high salivary levels of IgA antibodies were detected after oral and nasal immunizations. Nonimmunized control groups did not show any detectable humoral immune responses to FBP54 in sera (Kawabata et al., 2001).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: An enzyme-linked immunosorbent assay revealed that antigen-specific IgG antibodies were induced in the sera of immunized mice, while high salivary levels of IgA antibodies were detected after oral and nasal immunizations. Nonimmunized control groups did not show any detectable humoral immune responses to FBP54 in sera (Kawabata et al., 2001).
    • Detailed Gene Information: Click here.
4. S. pyogenes M Protein Vaccine
a. Vaccine Ontology ID:
VO_0004053
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
A polypeptide encompassing a conformational B cell epitope of the M protein conjugated with DT (Schulze et al., 2006).
e. Gene Engineering of emm1
  • Type: Recombinant protein preparation
  • Description: A polypeptide encompassing a conformational B cell epitope of the M protein (J8, positions 344–355 of the M1 sequence, accession no. X62131) was synthesised, purified and conjugated with DT (Schulze et al., 2006).
  • Detailed Gene Information: Click here.
f. Adjuvant:
g. Immunization Route
intranasal immunization
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunised by intranasal route (10 μl per nostril) with either J8-DT (M protein) (30 μg per dose) co-administered with 10 μg of CTB or 0.5 μg of the TLR2/6 agonist MALP-2 as mucosal adjuvants, on day 0, 7, 14, and 28. Control animals received PBS alone (Schulze et al., 2006).
  • Challenge Protocol: Mice were intranasally challenged with 10^8 CFU of the heterologous virulent S. pyogenes strain NS192 in 40 μl of PBS (20 μl per nostril) (Schulze et al., 2006).
  • Efficacy: Mice immunized with J8-DT had 60% survival following challenge (Schulze et al., 2006).
5. S. pyogenes SfbI Protein Vaccine
a. Vaccine Ontology ID:
VO_0004056
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Recombinant SfbI protein
e. Gene Engineering of SfbI
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant:
g. Immunization Route
intranasal immunization
h. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: MIce were immunized intranasally with 20 μL of SfbI (H2 fragment) covalently coupled to CTB or with CTB alone as a control on days 1, 3, 5, and 26 (30 mg/dose) (Guzmán et al., 1999).
  • Challenge Protocol: On day 36 mice were challenged with 10^6 cfu of S. pyogenes DSM 2071 or NS239, respectively (Guzmán et al., 1999).
  • Efficacy: SfbI-vaccinated animals showed 80% and 90% protection against homologous and heterologous challenge (Guzmán et al., 1999).
6. S. pyogenes Sib35 Protein Vaccine
a. Vaccine Ontology ID:
VO_0004057
b. Type:
Subunit vaccine
c. Status:
Research
d. Antigen
Recombinant Sib35 protein
e. Gene Engineering of sib35
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
f. Adjuvant:
g. Immunization Route
subcutaneous injection
h. Mouse Response
  • Host Strain: BALB/c, C3H/HeN, C57/BL6 and CD1
  • Vaccination Protocol: Six-week-old female mice were separated into two groups, which were either subcutaneously immunized with rSib35 (50 μg) in Freund's complete adjuvant or injected with PBS in FCA on day 0, and given booster injections with rSib35 and Freund's incomplete adjuvant on days 14, 28, and 42 (Okamoto et al., 2005).
  • Challenge Protocol: Actively immunized mice were intraperitoneally challenged with an appropriate number of CFUs of strain SSI-1 on day 56 following primary immunization (Okamoto et al., 2005).
  • Efficacy: The survival rates of all four-mouse strains were significantly increased by Sib35 vaccination. 100% of the Sib35-immunized C3H/HeN mice survived the potentially lethal GAS challenge, whereas all non-vaccinated C3H/HeN mice died after the challenge (Okamoto et al., 2005).
  • Host Gene Response of IgG
    • Gene Response: Immunization of mice with Sib 35 induced significantly higher antigen-specific IgG antibodies in their sera beginning 28 days after immunization as compared to non immunized control mice (Okamoto et al., 2005).
    • Detailed Gene Information: Click here.
7. SL3261 expressing streptococcal M protein
a. Vaccine Ontology ID:
VO_0004676
b. Type:
Recombinant vector vaccine
c. Status:
Research
d. Host Species for Licensed Use:
Baboon
e. Preparation
The aroA strain of S. typhimurium SL3261 was transformed with a low-copy plasmid vector pMK207, which contains the cloned gene spm5 encoding streptococcal M protein, the major virulence factor of these organisms (Poirier et al., 1988).
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Vaccination Protocol: Mice were immunized orally with the pMK207-transformed Salmonella SL3261 (Poirier et al., 1988).
    .
  • Vaccine Immune Response Type: VO_0003057
  • Challenge Protocol: Immunized and control mice were challenged intraperitoneally with either the homologous M5 or heterologous M24 streptococci, or with wild-type S typhimurium SL1344 (Poirier et al., 1988).
  • Efficacy: The orally immunized mice were completely protected against both intranasal and intraperitoneal challenge infections with virulent S. typhimurium SL1344 or M5 streptococci (Poirier et al., 1988).
V. References
1. Bensi et al., 2012: Bensi G, Mora M, Tuscano G, Biagini M, Chiarot E, Bombaci M, Capo S, Falugi F, Manetti AG, Donato P, Swennen E, Gallotta M, Garibaldi M, Pinto V, Chiappini N, Musser JM, Janulczyk R, Mariani M, Scarselli M, Telford JL, Grifantini R, Norais N, Margarit I, Grandi G. Multi high-throughput approach for highly selective identification of vaccine candidates: the Group A Streptococcus case. Molecular & cellular proteomics : MCP. 2012; 11(6); M111.015693. [PubMed: 22286755].
2. Chen et al., 2016: Chen X, Li N, Bi S, Wang X, Wang B. Co-Activation of Th17 and Antibody Responses Provides Efficient Protection against Mucosal Infection by Group A Streptococcus. PloS one. 2016; 11(12); e0168861. [PubMed: 28030629].
3. Courtney et al., 2017: Courtney HS, Niedermeyer SE, Penfound TA, Hohn CM, Greeley A, Dale JB. Trivalent M-related protein as a component of next generation group A streptococcal vaccines. Clinical and experimental vaccine research. 2017; 6(1); 45-49. [PubMed: 28168173].
4. Dale et al., 2015: Dale JB, Niedermeyer SE, Agbaosi T, Hysmith ND, Penfound TA, Hohn CM, Pullen M, Bright MI, Murrell DS, Shenep LE, Courtney HS. Protective immunogenicity of group A streptococcal M-related proteins. Clinical and vaccine immunology : CVI. 2015; 22(3); 344-350. [PubMed: 25630406].
5. Guerino et al., 2011: Guerino MT, Postol E, Demarchi LM, Martins CO, Mundel LR, Kalil J, Guilherme L. HLA class II transgenic mice develop a safe and long lasting immune response against StreptInCor, an anti-group A streptococcus vaccine candidate. Vaccine. 2011; 29(46); 8250-8256. [PubMed: 21907752].
6. Guzmán et al., 1999: Guzmán CA, Talay SR, Molinari G, Medina E, Chhatwal GS. Protective immune response against Streptococcus pyogenes in mice after intranasal vaccination with the fibronectin-binding protein SfbI. The Journal of infectious diseases. 1999; 179(4); 901-906. [PubMed: 10068585].
7. Kagawa et al., 2009: Kagawa TF, O'Connell MR, Mouat P, Paoli M, O'Toole PW, Cooney JC. Model for substrate interactions in C5a peptidase from Streptococcus pyogenes: A 1.9 A crystal structure of the active form of ScpA. Journal of molecular biology. 2009; 386(3); 754-772. [PubMed: 19152799].
8. Kawabata et al., 2001: Kawabata S, Kunitomo E, Terao Y, Nakagawa I, Kikuchi K, Totsuka K, Hamada S. Systemic and mucosal immunizations with fibronectin-binding protein FBP54 induce protective immune responses against Streptococcus pyogenes challenge in mice. Infection and immunity. 2001; 69(2); 924-930. [PubMed: 11159987].
9. Kawabata et al., 2001: Kawabata S, Kunitomo E, Terao Y, Nakagawa I, Kikuchi K, Totsuka K, Hamada S. Systemic and mucosal immunizations with fibronectin-binding protein FBP54 induce protective immune responses against Streptococcus pyogenes challenge in mice. Infection and immunity. 2001; 69(2); 924-930. [PubMed: 11159987].
10. Kotloff et al., 2004: Kotloff KL, Corretti M, Palmer K, Campbell JD, Reddish MA, Hu MC, Wasserman SS, Dale JB. Safety and immunogenicity of a recombinant multivalent group a streptococcal vaccine in healthy adults: phase 1 trial. JAMA : the journal of the American Medical Association. 2004; 292(6); 709-715. [PubMed: 15304468].
11. Lei et al., 2004: Lei B, Liu M, Chesney GL, Musser JM. Identification of new candidate vaccine antigens made by Streptococcus pyogenes: purification and characterization of 16 putative extracellular lipoproteins. The Journal of infectious diseases. 2004; 189(1); 79-89. [PubMed: 14702157].
12. Ma et al., 2009: Ma CQ, Li CH, Wang XR, Zeng RH, Yin XL, Feng HD, Wei L. Similar ability of FbaA with M protein to elicit protective immunity against group A streptococcus challenge in mice. Cellular & molecular immunology. 2009; 6(1); 73-77. [PubMed: 19254483].
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