VIOLIN: Vaccine Investigation and Online Information Network

Mycobacterium tuberculosis

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. SodA
2. Ag85B from M. tuberculosis H37Rv (Protective antigen)
3. Apa (Protective antigen)
4. bfrA (Protective antigen)
5. DnaK (Protective antigen)
6. EsxA (ESAT-6) (Protective antigen)
7. EsxB (Protective antigen)
8. esxV (Protective antigen)
9. esxW (Protective antigen)
10. FbpA (Ag85A) (Protective antigen)
11. FbpD (Protective antigen)
12. HBHA (Protective antigen)
13. KatG (Protective antigen)
14. Mpt63 (Protective antigen)
15. MPT64 (Protective antigen)
16. MPT83 (Protective antigen)
17. Mtb72F (Protective antigen)
18. PE20 (Protective antigen)
19. PepA (Protective antigen)
20. PPE14 (Protective antigen)
21. PPE18 (Protective antigen)
22. PPE31 (Protective antigen)
23. PPE42 (Protective antigen)
24. PstS1 (Protective antigen)
25. PstS3 (Protective antigen)
26. Rv1813c (Protective antigen)
27. Rv2660c (Protective antigen)
28. drrC (Virmugen)
29. fadD26 (Virmugen)
30. leuD (Virmugen)
31. lysA (Virmugen)
32. mce-2 (Virmugen)
33. mce-3 (Virmugen)
34. panC (Virmugen)
35. panD (Virmugen)
36. PhoP (Virmugen)
37. proC (Virmugen)
38. RD1 (Virmugen)
39. SecA2 (Virmugen)
40. sigE (Virmugen)
41. sigF (Virmugen)
42. trpD (Virmugen)
Vaccine Related Host Genes
1. APOBEC3B
2. C19orf66
3. CD70
4. Cxcl10
5. Cxcl11
6. Cxcl9
7. Defb3
8. Defb4
9. Ebi3
10. Fth1
11. Ftl
12. GPR182
13. GPX1
14. HIST1H2BE
15. HIST2H4A
16. human IFNG
17. IFNG
18. Ifng (Interferon gamma)
19. IL-1b
20. IL-6
21. IL10
22. IL10
23. Il10 (interleukin 10)
24. Il12b
25. Il15
26. Il17f
27. IL2
28. Il2
29. Il4 (interleukin 4)
30. IL6
31. lipY
32. Ltf
33. Nos2
34. PCDHB1
35. PTCD1
36. SLC11A1
37. STAG3
38. TBX21
39. TLR1
40. TNF-alpha
41. TSC22D4
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

1773

2. Disease:

Tuberculosis

3. Introduction

At the end of 19th century, Robert Koch discovered that Mycobacterium tuberculosis was the causative agent of tuberculosis (TB). TB is a major cause of morbidity and mortality throughout the world despite more than a half century of widespread vaccination, claiming millions of lives every year. The extraordinary high prevalence of the latent infection [estimated at one-third of the world's population] is one of the main factors contributing to the high incidence of active tuberculosis. The other factor is convergence of the TB and HIV epidemics. With HIV/AIDS fueling the TB crisis, the WHO has estimated that more than 1 billion people will be infected with TB bacilli in the next 20 years (Gupta et al., 2007).
M. tuberculosis are Gram-positive bacilli lacking both flagella and capsules. M. tuberculosis is preferentially established in the pulmonary system in a dormant state after initial infection via aerosol transmission. The M. tuberculosis complex, a group of Mycobacterium including M. tuberculosis, M. bovis, M. bovis BCG, M. africanum, and M. microti, are tuberculosis-inducing bacteria which have low horizontal gene transfer and variable levels of virulence (Ducati et al., 2006).

4. Microbial Pathogenesis

Mouse and guinea pig models have been used extensively to understand the host and pathogen-specific factors controlling pathogenesis of tuberculosis. Transmission of M. tuberculosis via intravenous route in murine models is followed by initial bacterial replication and subsequent macrophage activation via IL-6, IL-12, and TNF-mediated mechanisms. Bacterial load is significantly reduced at 2 weeks post-infection in liver and spleen. After load reduction, selected lymphocytes are activated which can then induce macrophages to begin producing nitric oxide via the iNOS pathway. Bacteria which survive the initial clearance by the host enter a viable yet non-replicating persistence stage. Factors such as immune suppression from aging, iNOS inhibition, and corticosteroid therapy can shift the dormant bacilli back into a replicative state. As an example of preferred host niche, transmission via inhalation will sometimes result in bacilli establishing infection in macrophage phagosomes in the lung. These macrophages become lodged in the calcified tubercles, and can later initiate formation of granulomas through a CD4-mediated delayed-type hypersensitivity reaction. Each granuloma can remain dormant, reduce in size and possibly disappear, initiate progressive interstitial fibrosis in lung tissue, and/or necrotize (Ernst et al., 2007). Similar mechanisms are observed in liver tissue (Ducati et al., 2006).
Numerous pathogen- and host-specific factors related to pathogenesis of tuberculosis are potential vaccine targets. For example, the Bacille-Calmette Guiren (BCG) vaccine was obtained from an M. bovine strain which lost its virulence after 39 passages (Ducati et al., 2006). Evidence from subtractive hybridization experiments, along with whole-genome microarrays and BAC arrays and other experimental evidence, suggests that the RD1 locus is the main virulence feature that is absent from 13 strains of avirulent BCG yet present in hundreds of other M. tuberculosis strains (Ernst et al., 2007). RD1 is related to the regulation of the early secreted antigen 6 kDa [ESAT-6] secretion system 1[ESX-1], an important pathway which is linked to the recruitment and infection of macrophages to initially infected sites and subsequent disease propagation (Ernst et al., 2007).

5. Host Ranges and Animal Models

Host ranges extend to many animals, including human, mouse, guinea pig, rabbit, and cattle. The most predominant animal used to study M. tuberculosis for virulence assessment and vaccine development is likely the mouse, which include C57BL/6 and BALB/c strains, mainly due to cost effectiveness for long-term vaccination/challenge studies. Dunkin Hartley guinea pigs are often used in M. tuberculosis studies as a close model for the overall pathogenesis in humans.

6. Host Protective Immunity

Although it is known that IFN-gamma is essential for protective immunity, animal and human studies have found that IFN-gamma alone is not sufficient for the prevention of TB disease. There is evidence that IL-23, a recently described member of the IL-12 family of cytokines, is important in the immuno-pathogenesis of TB. There is also evidence that regulatory T cells (Treg) are present in TB disease and that Treg may suppress effector T cell responses (Fletcher, 2007). New vaccines seek to target specific CD1, CD4+, and CD8+ T and B cell responses to assist in eradicating the disease via improved clearance of infected cells and bacterial loads.

II. Vaccine Related Pathogen Genes

1. Ag85B from M. tuberculosis H37Rv

Gene Name : Ag85B from M. tuberculosis H37Rv
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0012364
NCBI Gene ID : 885785
NCBI Protein GI : 15609023
Locus Tag : Rv1886c
Genbank Accession : AL123456
Protein Accession : NP_216402
Other Database IDs : EMBL: AE000516
EMBL: AF198032
EMBL: BX842578
EMBL: U38939
EMBL: X62398
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
InterPro: IPR000801
KEGG: mtc:MT1934
KEGG: mtu:Rv1886c
PDB: 1F0N
PDB: 1F0P
Pfam: PF00756
PIR: C70516
TIGR: MT1934
TubercuList: Rv1886c
UniProt: P31952
Taxonomy ID : 83332
Gene Starting Position : 2134889
Gene Ending Position : 2135866
Gene Strand (Orientation) : -
Protein Name : diacylglycerol acyltransferase/mycolyltransferase Ag85B
Protein pI : 5.72
Protein Weight : 33091.46
Protein Length : 325
Protein Note : Rv1886c, (MT1934, MTCY180.32), len: 325 aa. fbpB (alternate gene names: mpt59, 85B), precursor of the 85-B antigen (fibronectin-binding protein B) (mycolyl transferase 85B) (EC 2.3.1.-) (see citations below), highly similar to other Mycobacterial antigen precursors e.g. P12942|A85B_MYCBO ANTIGEN 85-B PRECURSOR from Mycobacterium bovis (323 aa); P21160|A85B_MYCKA ANTIGEN 85-B PRECURSOR from Mycobacterium kansasii (325 aa); etc. Also highly similar to Mycobacterium tuberculosis antigen precursors: Rv3804c|fbpA (338 aa), Rv0129c|fbpC2 (340 aa), and Rv3803c|fbpC1 (299 aa). TBparse score is 0.912.; mpt59; 85B
Protein Annotation : FUNCTION: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity (UniProt: P31952)

SUBCELLULAR LOCATION: Secreted protein (UniProt: P31952)

SIMILARITY: Belongs to the mycobacterial A85 antigen family (UniProt: P31952)

DNA Sequence : Show Sequence

>NC_000962.3:2134889-2135866 Mycobacterium tuberculosis H37Rv, complete genome
GTCAGCCGGCGCCTAACGAACTCTGCAGGTCACCCTTCATGGCGTTGAGCTGAGCGCCCCAGTACTCCCA
GCTGTGCGTGCCGTTGGGCGGGAAGTTGAACACGGCGTTGTGCCCGCCCGCGGCGTTGTACGCATCCTGG
AACTTCAGGTTGCTGCTACGAACGAAGTTCTCCAAGAACTCGGCGGGTATGTTGGCACCGCCCAACTCGT
TCGGGGTGCCGTTCCCGCAATAAACCCATAGCCGGGTGTTGTTTGCGACCAGCTTGGGGATCTGCTGCGT
AGGGTCGTTGCGCTCCCATGCCGGGTCACTCGAGGGACCCCACATGTCTGCGGCCTTGTAACCGCCGGCG
TCACCCATCGCGAGGCCGATCAGGCTAGGCCCCATCCCCTGAGAGGGGTCCAGCAGGGCCGACAGCGAGC
CGGCGTAGATGAACTGCTGGGGGTGGTAGGCGGCCAAGATCATTGCCGACGAGCCGGCCATCGACAAGCC
GATTGCAGCGCTGCCGGTGGGCTTCACGGCCCTGTTGGCGGACAACCATTGCGGCAGCTCGCTGGTCAGG
AAGGTTTCCCACTTGTAAGTCTGGCAGCCAGCCTTACCGCAGGCCGGGCTGTACCAGTCGCTGTAGAAGC
TGGACTGCCCGCCGACCGGCATGACTATCGACAGTCCCGACTGGTAGTACCACTCGAACGCCGGGGTGTT
GATATCCCAGCCGTTGTAGTCGTCTTGGGCGCGCAGGCCGTCGAGCAGATAAACCGCAGGTGAGTTGTTC
CCACCGCTCTGGAACTGAACCTTGATGTCGCGGCCCATCGACGGCGACGGCACCTGCAGGTACTCGACCG
GCAGCCCCGGCCGGGAGAACGCGCCCGCGGTTGCCGCTCCGCCGGCAAGCCCCACCAGGCCCGGAAGGAC
TACAGCCGCTGCCGTGCCGATCATCAATCGGCGTCCCCAAGCTCGAATCTTTCGGCTCACGTCTGTCA

Protein Sequence : Show Sequence

>NP_216402.1 diacylglycerol acyltransferase/mycolyltransferase Ag85B [Mycobacterium tuberculosis H37Rv]
MTDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQSGG
NNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETF
LTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGD
AGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKF
QDAYNAAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGAG

Molecule Role : Protective antigen
Molecule Role Annotation : In mice, a primary vaccination with Ag85B-encoding plasmid DNA (DNA-85B) was protective against Mycobacterium tuberculosis (MTB) infection and associated with Ag85B-specific CD4+ T cells producing IFN-gamma and controlling intramacrophagic MTB growth (Palma et al., 2007).
Related Vaccine(s): Ag85B and ESAT-6 fusion protein , Arabinomannan-tetanus toxoid conjugate , LT-BCG- Ag85B /Rv3425 , M. tuberculosis DNA Vaccine encoding Ag85B Protein , M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83 , MVA/IL-15/5Mtb vaccine , rBCG-Ag85A/ Ag85B

2. Apa

Gene Name : Apa
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0011175
NCBI Gene ID : 885896
NCBI Protein GI : 57116926
Locus Tag : Rv1860
Genbank Accession : AL123456
Protein Accession : YP_177849
Taxonomy ID : 83332
Gene Starting Position : 2107735
Gene Ending Position : 2108712
Gene Strand (Orientation) : +
Protein Name : hypothetical protein
Protein pI : 4.7
Protein Weight : 30636.65
Protein Length : 325
Protein Note : Rv1860, (MT1908, MTCY359.0013), len: 325 aa. apa (alternate gene names: mpt32, modD), Ala-, Pro-rich 45/47 kDa secreted protein, very similar to P46842|N43L_MYCLE from Mycobacterium leprae (287 aa), FASTA scores: opt: 1166, E(): 0, (66.4% identity in 298 aa overlap). Known to be glycosylated fibronectin-binding protein (see some citations). CHANGES IN THE MANNOSYLATION PATTERN OF THIS PROTEIN AFFECT ITS ABILITY TO STIMULATE T-LYMPHOCYTE RESPONSE. MAJOR IMMUNODOMINANT ANTIGEN THAT HAS POTENTIAL AS A VACCINE AGAINST TUBERCULOSIS. APA-ELISA COULD BE USED IN DIAGNOSIS. TBparse score is 0.924.; mpt32; modD

DNA Sequence : Show Sequence

>NC_000962.3:2107735-2108712 Mycobacterium tuberculosis H37Rv, complete genome
CATGCATCAGGTGGACCCCAACTTGACACGTCGCAAGGGACGATTGGCGGCACTGGCTATCGCGGCGATG
GCCAGCGCCAGCCTGGTGACCGTTGCGGTGCCCGCGACCGCCAACGCCGATCCGGAGCCAGCGCCCCCGG
TACCCACAACGGCCGCCTCGCCGCCGTCGACCGCTGCAGCGCCACCCGCACCGGCGACACCTGTTGCCCC
CCCACCACCGGCCGCCGCCAACACGCCGAATGCCCAGCCGGGCGATCCCAACGCAGCACCTCCGCCGGCC
GACCCGAACGCACCGCCGCCACCTGTCATTGCCCCAAACGCACCCCAACCTGTCCGGATCGACAACCCGG
TTGGAGGATTCAGCTTCGCGCTGCCTGCTGGCTGGGTGGAGTCTGACGCCGCCCACTTCGACTACGGTTC
AGCACTCCTCAGCAAAACCACCGGGGACCCGCCATTTCCCGGACAGCCGCCGCCGGTGGCCAATGACACC
CGTATCGTGCTCGGCCGGCTAGACCAAAAGCTTTACGCCAGCGCCGAAGCCACCGACTCCAAGGCCGCGG
CCCGGTTGGGCTCGGACATGGGTGAGTTCTATATGCCCTACCCGGGCACCCGGATCAACCAGGAAACCGT
CTCGCTCGACGCCAACGGGGTGTCTGGAAGCGCGTCGTATTACGAAGTCAAGTTCAGCGATCCGAGTAAG
CCGAACGGCCAGATCTGGACGGGCGTAATCGGCTCGCCCGCGGCGAACGCACCGGACGCCGGGCCCCCTC
AGCGCTGGTTTGTGGTATGGCTCGGGACCGCCAACAACCCGGTGGACAAGGGCGCGGCCAAGGCGCTGGC
CGAATCGATCCGGCCTTTGGTCGCCCCGCCGCCGGCGCCGGCACCGGCTCCTGCAGAGCCCGCTCCGGCG
CCGGCGCCGGCCGGGGAAGTCGCTCCTACCCCGACGACACCGACACCGCAGCGGACCTTACCGGCCTG

Protein Sequence : Show Sequence

>YP_177849.1 hypothetical protein Rv1860 [Mycobacterium tuberculosis H37Rv]
MHQVDPNLTRRKGRLAALAIAAMASASLVTVAVPATANADPEPAPPVPTTAASPPSTAAAPPAPATPVAP
PPPAAANTPNAQPGDPNAAPPPADPNAPPPPVIAPNAPQPVRIDNPVGGFSFALPAGWVESDAAHFDYGS
ALLSKTTGDPPFPGQPPPVANDTRIVLGRLDQKLYASAEATDSKAAARLGSDMGEFYMPYPGTRINQETV
SLDANGVSGSASYYEVKFSDPSKPNGQIWTGVIGSPAANAPDAGPPQRWFVVWLGTANNPVDKGAAKALA
ESIRPLVAPPPAPAPAPAEPAPAPAPAGEVAPTPTTPTPQRTLPA

Molecule Role : Protective antigen
Molecule Role Annotation : We show that the 50- to 55-kDa Apa protein, specified by the Rv1860 gene of M. tuberculosis, can elicit both lymphoproliferative response and gamma interferon (IFN-gamma) production. A poxvirus recombinant expressing the Apa protein conferred a significant level of protective immunity in guinea pigs against a challenge dose of virulent M. tuberculosis (Kumar et al., 2003).
Related Vaccine(s): M. tuberculosis DNA Vaccine APADNA priming and APAMVA boosting

3. bfrA

Gene Name : bfrA
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 885767
NCBI Protein GI : 15609013
Locus Tag : Rv1876
Genbank Accession : AL123456
Protein Accession : NP_216392
Taxonomy ID : 83332
Gene Starting Position : 2125339
Gene Ending Position : 2125818
Gene Strand (Orientation) : +
Protein Name : bacterioferritin BfrA
Protein pI : 4.23
Protein Weight : 17744.75
Protein Length : 159
Protein Note : Also known as bfr

DNA Sequence : Show Sequence

>NC_000962.3:2125339-2125818 Mycobacterium tuberculosis H37Rv, complete genome
CATGCAAGGTGATCCCGATGTTCTGCGCCTGCTCAACGAACAATTGACCAGCGAGCTCACCGCTATCAAC
CAATACTTTCTGCACTCCAAGATGCAGGACAACTGGGGTTTTACCGAGCTGGCGGCCCACACCCGCGCGG
AGTCGTTCGACGAAATGCGGCACGCCGAGGAAATCACCGATCGCATCTTGTTGCTGGATGGTTTGCCGAA
CTACCAGCGCATCGGTTCGTTGCGTATCGGCCAGACGCTCCGCGAGCAATTTGAGGCCGATCTGGCGATC
GAATACGACGTGTTGAATCGTCTCAAGCCAGGAATCGTCATGTGCCGGGAGAAACAGGACACCACCAGCG
CCGTACTGCTGGAGAAAATCGTTGCCGACGAGGAAGAACACATCGACTACTTGGAAACGCAGCTGGAGCT
GATGGACAAGCTAGGAGAGGAGCTTTACTCGGCGCAGTGCGTCTCTCGCCCACCGACCTG

Protein Sequence : Show Sequence

>NP_216392.1 bacterioferritin BfrA [Mycobacterium tuberculosis H37Rv]
MQGDPDVLRLLNEQLTSELTAINQYFLHSKMQDNWGFTELAAHTRAESFDEMRHAEEITDRILLLDGLPN
YQRIGSLRIGQTLREQFEADLAIEYDVLNRLKPGIVMCREKQDTTSAVLLEKIVADEEEHIDYLETQLEL
MDKLGEELYSAQCVSRPPT

Molecule Role : Protective antigen
Molecule Role Annotation : Mycobacterium frA (or called MMP-II) is one of the immunodominant antigens (Mukai et al., 2014).
Related Vaccine(s): BCG-DHTM

4. DnaK

Gene Name : DnaK
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0012408
NCBI Gene ID : 885946
NCBI Protein GI : 15607491
Locus Tag : Rv0350
Genbank Accession : AL123456
Protein Accession : NP_214864
Taxonomy ID : 83332
Gene Starting Position : 419834
Gene Ending Position : 421711
Gene Strand (Orientation) : +
Protein Name : chaperone protein DnaK
Protein pI : 4.59
Protein Weight : 61176.37
Protein Length : 625
Protein Note : heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria

DNA Sequence : Show Sequence

>NC_000962.3:419834-421711 Mycobacterium tuberculosis H37Rv, complete genome
CATGGCTCGTGCGGTCGGGATCGACCTCGGGACCACCAACTCCGTCGTCTCGGTTCTGGAAGGTGGCGAC
CCGGTCGTCGTCGCCAACTCCGAGGGCTCCAGGACCACCCCGTCAATTGTCGCGTTCGCCCGCAACGGTG
AGGTGCTGGTCGGCCAGCCCGCCAAGAACCAGGCAGTGACCAACGTCGATCGCACCGTGCGCTCGGTCAA
GCGACACATGGGCAGCGACTGGTCCATAGAGATTGACGGCAAGAAATACACCGCGCCGGAGATCAGCGCC
CGCATTCTGATGAAGCTGAAGCGCGACGCCGAGGCCTACCTCGGTGAGGACATTACCGACGCGGTTATCA
CGACGCCCGCCTACTTCAATGACGCCCAGCGTCAGGCCACCAAGGACGCCGGCCAGATCGCCGGCCTCAA
CGTGCTGCGGATCGTCAACGAGCCGACCGCGGCCGCGCTGGCCTACGGCCTCGACAAGGGCGAGAAGGAG
CAGCGAATCCTGGTCTTCGACTTGGGTGGTGGCACTTTCGACGTTTCCCTGCTGGAGATCGGCGAGGGTG
TGGTTGAGGTCCGTGCCACTTCGGGTGACAACCACCTCGGCGGCGACGACTGGGACCAGCGGGTCGTCGA
TTGGCTGGTGGACAAGTTCAAGGGCACCAGCGGCATCGATCTGACCAAGGACAAGATGGCGATGCAGCGG
CTGCGGGAAGCCGCCGAGAAGGCAAAGATCGAGCTGAGTTCGAGTCAGTCCACCTCGATCAACCTGCCCT
ACATCACCGTCGACGCCGACAAGAACCCGTTGTTCTTAGACGAGCAGCTGACCCGCGCGGAGTTCCAACG
GATCACTCAGGACCTGCTGGACCGCACTCGCAAGCCGTTCCAGTCGGTGATCGCTGACACCGGCATTTCG
GTGTCGGAGATCGATCACGTTGTGCTCGTGGGTGGTTCGACCCGGATGCCCGCGGTGACCGATCTGGTCA
AGGAACTCACCGGCGGCAAGGAACCCAACAAGGGCGTCAACCCCGATGAGGTTGTCGCGGTGGGAGCCGC
TCTGCAGGCCGGCGTCCTCAAGGGCGAGGTGAAAGACGTTCTGCTGCTTGATGTTACCCCGCTGAGCCTG
GGTATCGAGACCAAGGGCGGGGTGATGACCAGGCTCATCGAGCGCAACACCACGATCCCCACCAAGCGGT
CGGAGACTTTCACCACCGCCGACGACAACCAACCGTCGGTGCAGATCCAGGTCTATCAGGGGGAGCGTGA
GATCGCCGCGCACAACAAGTTGCTCGGGTCCTTCGAGCTGACCGGCATCCCGCCGGCGCCGCGGGGGATT
CCGCAGATCGAGGTCACTTTCGACATCGACGCCAACGGCATTGTGCACGTCACCGCCAAGGACAAGGGCA
CCGGCAAGGAGAACACGATCCGAATCCAGGAAGGCTCGGGCCTGTCCAAGGAAGACATTGACCGCATGAT
CAAGGACGCCGAAGCGCACGCCGAGGAGGATCGCAAGCGTCGCGAGGAGGCCGATGTTCGTAATCAAGCC
GAGACATTGGTCTACCAGACGGAGAAGTTCGTCAAAGAACAGCGTGAGGCCGAGGGTGGTTCGAAGGTAC
CTGAAGACACGCTGAACAAGGTTGATGCCGCGGTGGCGGAAGCGAAGGCGGCACTTGGCGGATCGGATAT
TTCGGCCATCAAGTCGGCGATGGAGAAGCTGGGCCAGGAGTCGCAGGCTCTGGGGCAAGCGATCTACGAA
GCAGCTCAGGCTGCGTCACAGGCCACTGGCGCTGCCCACCCCGGCGGCGAGCCGGGCGGTGCCCACCCCG
GCTCGGCTGATGACGTTGTGGACGCGGAGGTGGTCGACGACGGCCGGGAGGCCAAGTG

Protein Sequence : Show Sequence

>NP_214864.1 chaperone protein DnaK [Mycobacterium tuberculosis H37Rv]
MARAVGIDLGTTNSVVSVLEGGDPVVVANSEGSRTTPSIVAFARNGEVLVGQPAKNQAVTNVDRTVRSVK
RHMGSDWSIEIDGKKYTAPEISARILMKLKRDAEAYLGEDITDAVITTPAYFNDAQRQATKDAGQIAGLN
VLRIVNEPTAAALAYGLDKGEKEQRILVFDLGGGTFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVD
WLVDKFKGTSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINLPYITVDADKNPLFLDEQLTRAEFQR
ITQDLLDRTRKPFQSVIADTGISVSEIDHVVLVGGSTRMPAVTDLVKELTGGKEPNKGVNPDEVVAVGAA
LQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTRLIERNTTIPTKRSETFTTADDNQPSVQIQVYQGERE
IAAHNKLLGSFELTGIPPAPRGIPQIEVTFDIDANGIVHVTAKDKGTGKENTIRIQEGSGLSKEDIDRMI
KDAEAHAEEDRKRREEADVRNQAETLVYQTEKFVKEQREAEGGSKVPEDTLNKVDAAVAEAKAALGGSDI
SAIKSAMEKLGQESQALGQAIYEAAQAASQATGAAHPGGEPGGAHPGSADDVVDAEVVDDGREAK

Molecule Role : Protective antigen
Molecule Role Annotation : In an ex vivo transfection approach with a retroviral vector a single antigen (hsp70) could evoke strong protection in mice (Lowrie et al., 1997).
Related Vaccine(s): BCG-DHTM

5. drrC

Gene Name : drrC
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 888491
NCBI Protein GI : 15610075
Locus Tag : Rv2938
Genbank Accession : BX842581
Protein Accession : NP_217454
Taxonomy ID : 83332
Gene Starting Position : 3274071
Gene Ending Position : 3274901
Gene Strand (Orientation) : ?
Protein Name : daunorubicin-DIM-transport integral membrane protein ABC transporter DrrC
Protein pI : 10.46
Protein Weight : 26320.25
Protein Length : 276
Protein Note : Rv2938, (MTCY19H9.06), len: 276 aa. Probable drrC, daunorubicin-DIM-transport integral membrane protein ABC transporter, probably involved in daunorubicin resistance and phthiocerol dimycocerosate transport (see citations below), equivalent to Q9CB71|ML2350 PROBABLE ANTIBIOTIC RESISTANCE MEMBRANE PROTEIN from Mycobacterium leprae (276 aa), FASTA scores: opt: 1434, E(): 1.2e-81, (79.0% identity in 276 aa overlap); and Q49941|DRRC|L518_F3_76 PUTATIVE DAUNORUBICIN RESISTANCE TRANSMEMBRANE PROTEIN from Mycobacterium leprae (244 aa), FASTA scores: opt: 1194, E(): 8.3e-67, (76.85% identity in 242 aa overlap). Also similar to others e.g. Q9XCF9 DRRC PROTEIN from Mycobacterium avium (263 aa), FASTA scores: opt: 538, E(): 3.7e-26, (32.65% identity in 251 aa overlap); Q9S6H3 DAUNORUBICIN RESISTANCE PROTEIN C from Mycobacterium avium (263 aa), FASTA scores: opt: 533, E(): 7.6e-26, (32.25% identity in 251 aa overlap); P32011|DRRB_STRPE DAUNORUBICIN RESISTANCE TRANSMEMBRANE PROTEIN from Streptomyces peucetius (283 aa), FASTA scores: opt: 276, E(): 6.6e-10, (21.07% identity in 261 aa overlap); etc. Note that Rv2938|drrC belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentaly).

DNA Sequence : Show Sequence

>gi|57116681:3274071-3274901 Mycobacterium tuberculosis H37Rv, complete genome
CATGATCACGACGACAAGTCAGGAAATCGAGCTTGCACCCACACGTTTGCCAGGCTCGCAAAACGCTGCT
CGGCTGTTCGTTGCGCAGACCCTTTTGCAGACCAACCGGTTGCTAACTCGATGGGCACGTGACTATATCA
CCGTTATCGGAGCGATCGTGTTACCGATTCTCTTCATGGTGGTGTTGAACATTGTGCTAGGTAACCTAGC
TTATGTCGTAACCCACGACAGCGGGCTCTACAGCATTGTTCCGCTGATCGCACTCGGCGCCGCGATCACT
GGGTCAACTTTTGTCGCGATCGACCTGATGCGCGAGCGCTCCTTCGGACTGCTTGCCCGACTGTGGGTGC
TGCCCGTGCACCGAGCATCGGGCCTGATCTCTCGAATCCTGGCAAACGCGATTCGGACTCTGGTCACCAC
TTTAGTGATGCTAGGTACTGGGGTGGTATTGGGTTTCCGGTTTCGACAAGGCCTGATCCCGAGCCTCATG
TGGATTAGTGTCCCGGTGATACTGGGCATCGCAATCGCGGCTATGGTCACTACCGTCGCGCTTTACACAG
CACAAACCGTTGTTGTCGAAGGCGTTGAGCTGGTGCAAGCAATCGCGATCTTCTTCTCCACGGGTTTGGT
GCCGCTCAACTCGTATCCAGGCTGGATTCAGCCGTTCGTCGCCCATCAGCCGGTGAGCTACGCCATCGCG
GCGATGCGCGGTTTTGCAATGGGTGGTCCGGTCCTCTCTCCGATGATCGGGATGCTGGTGTGGACCGCGG
GTATCTGCGTCGTATGCGCCGTACCCTTGGCCATTGGCTACCGACGGGCCAGCACGCATTG

Protein Sequence : Show Sequence

>gi|15610075|ref|NP_217454.1| daunorubicin-DIM-transport integral membrane protein ABC transporter DrrC [Mycobacterium tuberculosis H37Rv]
MITTTSQEIELAPTRLPGSQNAARLFVAQTLLQTNRLLTRWARDYITVIGAIVLPILFMVVLNIVLGNLA
YVVTHDSGLYSIVPLIALGAAITGSTFVAIDLMRERSFGLLARLWVLPVHRASGLISRILANAIRTLVTT
LVMLGTGVVLGFRFRQGLIPSLMWISVPVILGIAIAAMVTTVALYTAQTVVVEGVELVQAIAIFFSTGLV
PLNSYPGWIQPFVAHQPVSYAIAAMRGFAMGGPVLSPMIGMLVWTAGICVVCAVPLAIGYRRASTH

Molecule Role : Virmugen
Molecule Role Annotation : A drrC mutant is highly attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis (Pinto et al., 2004).
Related Vaccine(s): Mycobacterium tuberculosis drrC mutant vaccine

6. EsxA (ESAT-6)

Gene Name : EsxA (ESAT-6)
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010919
NCBI Gene ID : 886209
NCBI Protein GI : 57117165
Locus Tag : Rv3875
Genbank Accession : AL123456
Protein Accession : YP_178023
Other Database IDs : EMBL: AE000516
EMBL: AF004671
EMBL: AF420491
EMBL: BX842584
EMBL: X79562
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
InterPro: IPR010310
KEGG: mtc:MT3989
KEGG: mtu:Rv3875
PDB: 1WA8
Pfam: PF06013
PIR: A70803
TIGR: MT3989
TubercuList: Rv3875
UniProt: P0A564
Taxonomy ID : 83332
Gene Starting Position : 4352608
Gene Ending Position : 4352895
Gene Strand (Orientation) : +
Protein Name : ESAT-6 protein EsxA
Protein pI : 4.19
Protein Weight : 9605.81
Protein Length : 95
Protein Note : Rv3875, (MT3989, MTV027.10), len: 95 aa. esxA, early secretory antigenic target (see citations below), identical to Q57165|O84901|ESAT6 EARLY SECRETORY ANTIGENIC TARGET from Mycobacterium bovis (94 aa), FASTA scores: opt: 596, E(): 4.6e-33, (100.0% identity in 94 aa overlap). Also similar to Q50206|ESA6_MYCLE|ESAT6|ESX|L45|ML0049|MLCB628.12c 6 KDA EARLY SECRETORY ANTIGENIC TARGET HOMOLOG (ESAT-6-LIKE PROTEIN) (L-ESAT) from Mycobacterium leprae (95 aa), FASTA scores: opt: 236, E(): 3.3e-09, (36.25% identity in 91 aa overlap); and weak similarity with others proteins ESAT-like from Mycobacterium leprae. Also some similarity with O53266|ES69_MYCTU|Rv3019c|MT3104|MTV012.33c PUTATIVE SECRETED ESAT-6 LIKE PROTEIN 9 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 131, E(): 0.03, (26.15% identity in 88 aa overlap); and other ESAT-like protein. Contains probable coiled-coil from 56 to 92 aa. BELONGS TO THE ESAT6 FAMILY. Note that previously known as esat-6.; esat-6
Protein Annotation : FUNCTION: Not known. Elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response (UniProt: P0A564)

SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A564)

SIMILARITY: Belongs to the ESAT-6 (esx) family (UniProt: P0A564)

DNA Sequence : Show Sequence

>NC_000962.3:4352608-4352895 Mycobacterium tuberculosis H37Rv, complete genome
CATGACAGAGCAGCAGTGGAATTTCGCGGGTATCGAGGCCGCGGCAAGCGCAATCCAGGGAAATGTCACG
TCCATTCATTCCCTCCTTGACGAGGGGAAGCAGTCCCTGACCAAGCTCGCAGCGGCCTGGGGCGGTAGCG
GTTCGGAGGCGTACCAGGGTGTCCAGCAAAAATGGGACGCCACGGCTACCGAGCTGAACAACGCGCTGCA
GAACCTGGCGCGGACGATCAGCGAAGCCGGTCAGGCAATGGCTTCGACCGAAGGCAACGTCACTGGGATG
TTCGCATA

Protein Sequence : Show Sequence

>YP_178023.1 ESAT-6 protein EsxA [Mycobacterium tuberculosis H37Rv]
MTEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQ
NLARTISEAGQAMASTEGNVTGMFA

Molecule Role : Protective antigen
Molecule Role Annotation : The heterologous prime-boost strategy of live Salmonella vaccine expressing Mycobacterium tuberculosis fusion antigen Age5B-ESAT6 (esxA) from the chromosome followed by a systemic boost of antigen and adjuvant reduced the levels of M. tuberculosis bacteria in the lungs and spleen to the same extent as BCG. Additionally, this vaccination regimen was observed to be statistically equivalent in terms of protection to immunisation with BCG (Hall et al., 2009).
In a prophylactic vaccine model, immunization with the Mycobacterium tuberculosis protein ESAT-6 with LANAC adjuvant elicited significant protective immunity against aerosol challenge with virulent M. tuberculosis (Zaks et al., 2006).
Related Vaccine(s): Ag85B and ESAT-6 fusion protein , attenuated M. bovis strain WAg539 , MVA/IL-15/5Mtb vaccine , recombinant S. typhimurium secreting M. tuberculosis ESAT-6

7. EsxB

Gene Name : EsxB
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010951
NCBI Gene ID : 886194
NCBI Protein GI : 15611010
Locus Tag : Rv3874
Genbank Accession : AF004671
Protein Accession : NP_218391
3D structure: PDB ID : 3FAV
Other Database IDs : EMBL: AE000516
EMBL: AF004671
EMBL: AF419854
EMBL: BX842584
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
InterPro: IPR010310
KEGG: mtc:MT3988
KEGG: mtu:Rv3874
Pfam: PF06013
PIR: H70802
SMR: P0A566
TIGR: MT3988
TubercuList: Rv3874
UniProt: P0A566
Taxonomy ID : 83332
Gene Starting Position : 4352273
Gene Ending Position : 4352575
Gene Strand (Orientation) : +
Protein Name : ESAT-6-like protein EsxB
Protein pI : 4.31
Protein Weight : 10396.66
Protein Length : 100
Protein Note : Rv3874, (MT3988, MTV027.09), len: 100 aa. esxB, 10 KDA culture filtrate antigen (see citations below, especially first), highly similar to O33084|CF10_MYCLE|ML0050|MLCB628.13c 10 KDA CULTURE FILTRATE ANTIGEN CFP10 HOMOLOG from Mycobacterium leprae (99 aa), FASTA scores: opt: 237, E(): 2.4e-08, (39.4% identity in 99 aa overlap). Also similar to O05440|ES6D_MYCTU|Rv3905c|MT4024|MTCY15F10.06 PUTATIVE ESAT-6 LIKE PROTEIN 13 from Mycobacterium tuberculosis (103 aa) FASTA scores: opt: 126, E(): 0.18, (23.1% identity in 91 aa overlap); and shows some similarity with other proteins from Mycobacterium tuberculosis. Contains probable coiled-coil from aa 49-93. BELONGS TO THE ESAT6 FAMILY. Note that previously known as lhp (alternate gene name: cfp10).; lhp, cfp10
Protein Annotation : SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A566)

SIMILARITY: Belongs to the ESAT-6 (esx) family (UniProt: P0A566)

DNA Sequence : Show Sequence

>NC_000962.3:4352273-4352575 Mycobacterium tuberculosis H37Rv, complete genome
CATGGCAGAGATGAAGACCGATGCCGCTACCCTCGCGCAGGAGGCAGGTAATTTCGAGCGGATCTCCGGC
GACCTGAAAACCCAGATCGACCAGGTGGAGTCGACGGCAGGTTCGTTGCAGGGCCAGTGGCGCGGCGCGG
CGGGGACGGCCGCCCAGGCCGCGGTGGTGCGCTTCCAAGAAGCAGCCAATAAGCAGAAGCAGGAACTCGA
CGAGATCTCGACGAATATTCGTCAGGCCGGCGTCCAATACTCGAGGGCCGACGAGGAGCAGCAGCAGGCG
CTGTCCTCGCAAATGGGCTTCTG

Protein Sequence : Show Sequence

>NP_218391.1 ESAT-6-like protein EsxB [Mycobacterium tuberculosis H37Rv]
MAEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELD
EISTNIRQAGVQYSRADEEQQQALSSQMGF

Molecule Role : Protective antigen
Molecule Role Annotation : The 10-kDa culture filtrate protein (CFP-10, esxB) and 6-kDa early secretory antigen of T cells (ESAT-6) are secreted in abundance by Mycobacterium tuberculosis. Study demonstrated that protective immunity is induced by CFP-10 DNA vaccination in C3H mice as measured by a CFU reduction in the lung and spleen 4 and 8 weeks after challenge with M. tuberculosis (Wu et al., 2008).
Related Vaccine(s): attenuated M. bovis strain WAg539

8. esxV

Gene Name : esxV
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Protein GI : NP_218136
Other Database IDs : CDD:310537
Taxonomy ID : 83332
Gene Strand (Orientation) : ?
Protein Name : ESAT-6 like protein EsxV
Protein pI : 4.62
Protein Weight : 10489.63
Protein Length : 170
Protein Note : Proteins of 100 residues with WXG; pfam06013

Protein Sequence : Show Sequence

>NP_218136.1 ESAT-6 like protein EsxV [Mycobacterium tuberculosis H37Rv]
MTINYQFGDVDAHGAMIRAQAGSLEAEHQAIISDVLTASDFWGGAGSAACQGFITQLGRNFQVIYEQANA
HGQKVQAAGNNMAQTDSAVGSSWA

Molecule Role : Protective antigen
Molecule Role Annotation : ID93/GLA-SE protects against TB and MDR-TB in animals.(Bertholet et al., 2010)
Related Vaccine(s): ID93/GLA-SE

9. esxW

Gene Name : esxW
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Protein GI : NP_218137
Other Database IDs : CDD:310537
Taxonomy ID : 83332
Gene Strand (Orientation) : ?
Protein Name : ESAT-6 like protein EsxW
Protein pI : 5.09
Protein Weight : 11866.28
Protein Length : 174
Protein Note : Proteins of 100 residues with WXG; pfam06013

Protein Sequence : Show Sequence

>NP_218137.1 ESAT-6 like protein EsxW [Mycobacterium tuberculosis H37Rv]
MTSRFMTDPHAMRDMAGRFEVHAQTVEDEARRMWASAQNISGAGWSGMAEATSLDTMTQMNQAFRNIVNM
LHGVRDGLVRDANNYEQQEQASQQILSS

Molecule Role : Protective antigen
Molecule Role Annotation : ID93/GLA-SE protects against TB and MDR-TB in animals.(Bertholet et al., 2010)
Related Vaccine(s): ID93/GLA-SE

10. fadD26

Gene Name : fadD26
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 887603
NCBI Protein GI : 57117038
Locus Tag : Rv2930
Genbank Accession : BX842581
Protein Accession : NP_217446
Taxonomy ID : 83332
Gene Starting Position : 3243696
Gene Ending Position : 3245447
Gene Strand (Orientation) : ?
Protein Name : acyl-CoA synthetase
Protein pI : 6.2
Protein Weight : 57587.87
Protein Length : 583
Protein Note : activates fatty acids by binding to coenzyme A

DNA Sequence : Show Sequence

>gi|57116681:3243696-3245447 Mycobacterium tuberculosis H37Rv, complete genome
GATGCCGGTGACCGACCGTTCAGTGCCCTCTTTGCTGCAAGAGAGGGCCGACCAGCAGCCTGACAGCACT
GCATATACGTACATCGACTACGGATCCGACCCCAAGGGATTTGCTGACAGCTTGACTTGGTCGCAGGTCT
ACAGTCGTGCATGCATCATTGCTGAAGAACTCAAGTTATGCGGGTTACCCGGAGATCGAGTGGCGGTTTT
AGCGCCACAAGGACTGGAATATGTCCTTGCATTCCTGGGCGCACTTCAGGCTGGATTTATCGCGGTTCCG
CTGTCAACTCCACAGTATGGCATTCACGATGACCGCGTTTCTGCGGTGTTGCAGGATTCCAAGCCGGTAG
CCATTCTCACGACTTCGTCCGTGGTAGGCGATGTAACGAAATACGCAGCCAGCCACGACGGGCAGCCTGC
CCCGGTCGTAGTTGAGGTTGATCTGCTTGATTTGGACTCGCCGCGACAGATGCCGGCTTTCTCTCGTCAG
CACACCGGGGCGGCTTATCTCCAATACACGTCCGGATCGACGCGTACGCCGGCCGGAGTCATTGTGTCGC
ACACGAATGTCATTGCCAATGTGACACAAAGTATGTACGGCTATTTCGGCGATCCCGCAAAGATTCCGAC
CGGGACTGTGGTGTCGTGGCTGCCTTTGTATCACGATATGGGCCTGATTCTCGGAATTTGCGCACCGCTG
GTGGCCCGACGCCGCGCGATGTTGATGAGCCCAATGTCATTTTTGCGCCGTCCGGCCCGCTGGATGCAAC
TGCTTGCCACCAGCGGCCGGTGCTTTTCTGCGGCACCGAATTTCGCCTTCGAGCTGGCCGTGCGCAGAAC
ATCTGACCAGGACATGGCGGGGCTCGACCTGCGCGACGTGGTCGGCATCGTCAGTGGCAGTGAGCGAATC
CATGTGGCAACCGTGCGGCGGTTCATCGAGCGGTTCGCGCCGTACAATCTCAGCCCCACCGCGATACGGC
CGTCGTACGGGCTCGCGGAAGCGACCTTATATGTGGCAGCTCCCGAAGCCGGCGCCGCGCCCAAGACGGT
CCGTTTTGACTACGAGCAGCTGACCGCCGGGCAGGCTCGGCCCTGCGGAACCGATGGGTCGGTCGGCACC
GAACTGATCAGCTACGGCTCCCCCGACCCATCGTCTGTGCGAATCGTCAACCCGGAGACCATGGTTGAGA
ATCCGCCTGGAGTGGTCGGTGAGATCTGGGTGCATGGCGACCACGTGACTATGGGGTATTGGCAGAAGCC
GAAGCAGACCGCGCAGGTCTTCGACGCCAAGCTGGTCGATCCCGCGCCGGCAGCCCCGGAGGGGCCGTGG
CTGCGCACCGGCGACCTGGGCGTCATTTCCGATGGTGAGCTGTTCATCATGGGCCGCATCAAAGACCTGC
TCATCGTGGACGGGCGCAACCACTACCCCGACGACATCGAGGCAACGATCCAGGAGATCACCGGTGGACG
GGCCGCGGCGATCGCAGTGCCCGACGACATCACCGAACAACTGGTGGCGATCATCGAATTCAAGCGACGC
GGTAGTACCGCCGAAGAGGTCATGCTCAAGCTCCGCTCGGTGAAGCGTGAGGTCACCTCCGCGATATCGA
AGTCACACAGCCTGCGGGTGGCCGATCTCGTTCTGGTGTCACCTGGTTCGATTCCCATCACCACCAGCGG
CAAGATCCGGCGGTCAGCCTGCGTCGAACGCTATCGCAGCGACGGCTTCAAGCGGCTGGACGTAGCCGTA
TG

Protein Sequence : Show Sequence

>gi|57117038|ref|NP_217446.2| acyl-CoA synthetase [Mycobacterium tuberculosis H37Rv]
MPVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCGLPGDRVAVL
APQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVAILTTSSVVGDVTKYAASHDGQPA
PVVVEVDLLDLDSPRQMPAFSRQHTGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQSMYGYFGDPAKIPT
GTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRRT
SDQDMAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTV
RFDYEQLTAGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKP
KQTAQVFDAKLVDPAPAAPEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIEATIQEITGGR
AAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSG
KIRRSACVERYRSDGFKRLDVAV

Molecule Role : Virmugen
Molecule Role Annotation : An fadD26 mutant is attenuated in mice and induced significant protection from challenge with wild type M. tuberculosis (Infante et al., 2005).
Related Vaccine(s): Mycobacterium tuberculosis fadD26 mutant vaccine

11. FbpA (Ag85A)

Gene Name : FbpA (Ag85A)
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010952
NCBI Gene ID : 886132
NCBI Protein GI : 15610940
Locus Tag : Rv3804c
Genbank Accession : AL123456
Protein Accession : NP_218321
3D structure: PDB ID : 1SFR
Other Database IDs : EMBL: AE000516
EMBL: BX842584
EMBL: M27016
EMBL: U47335
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
InterPro: IPR000801
KEGG: mtc:MT3911
KEGG: mtu:Rv3804c
PDB: 1SFR
Pfam: PF00756
PIR: H70887
TIGR: MT3911
TubercuList: Rv3804c
UniProt: P0A4V2
Taxonomy ID : 83332
Gene Starting Position : 4265641
Gene Ending Position : 4266657
Gene Strand (Orientation) : -
Protein Name : diacylglycerol acyltransferase/mycolyltransferase Ag85A
Protein pI : 6.51
Protein Weight : 33304.71
Protein Length : 338
Protein Note : Rv3804c, (MT3911, MTV026.09c), len: 338 aa. fbpA (alternate gene names: mpt44, 85A), precursor of the 85-A antigen (fibronectin-binding protein A) (mycolyl transferase 85A) (EC 2.3.1.-) (see citations below), identical to P17944|P17996|FBPA|MPT44 ANTIGEN 85-A PRECURSOR from Mycobacterium bovis (338 aa), FASTA scores: opt: 2341, E(): 1.2e-132, (100.0% identity in 338 aa overlap); and highly similar to other Mycobacterial antigen precursors e.g. O52956|A85A_MYCAV|FBPA ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium avium (347 aa), FASTA scores: opt: 1987, E(): 1.7e-111, (82.55% identity in 338 aa overlap); Q05861|A85A_MYCLE|FBPA|ML0097 ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium leprae (330 aa), FASTA scores: opt: 1936, E(): 1.9e-108, (83.0% identity in 329 aa overlap); O06052|A85A_MYCGO|FBPA ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium gordonae (339 aa), FASTA scores: opt: 1932, E(): 3.3e-108, (80.45% identity in 338 aa overlap); etc. Also highly similar to P31952|A85B_MYCTU|FBPB|Rv1886c|MT1934|MTCY180.32 SECRETED ANTIGEN 85-B from Mycobacterium tuberculosis (325 aa), FASTA scores: opt: 1830, E(): 3.9e-102, (78.85% identity in 317 aa overlap); P31953|A85C_MYCTU|FBPC|MPT45|Rv0129c|MTCI5.03c|FBPC2 SECRETED ANTIGEN 85-C from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 1597, E(): 3.4e-88, (67.25% identity in 336 aa overlap).; mpt44; 85A
Protein Annotation : FUNCTION: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity (UniProt: P0A4V2)

SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A4V2)

PTM: Contains one disulfide bridge (UniProt: P0A4V2)

SIMILARITY: Belongs to the mycobacterial A85 antigen family (UniProt: P0A4V2)

DNA Sequence : Show Sequence

>NC_000962.3:4265641-4266657 Mycobacterium tuberculosis H37Rv, complete genome
GCTAGGCGCCCTGGGGCGCGGGCCCGGTGTTGGGCGTGGCACCCAGTGCCCGTTGCAGGTCGGGCTTCAT
AGCGTTGAGCTGCGCGCCCCAGTACTCCCAGCTGTGCGTACCGCTGTCCGGGAAGTCGAACACGCCGTTG
TGGCCGCCACCGGCGTTGTAGGCGTCTTGGAACTTGATGTTGCTGGTCCGCACGAAGCCCTCGAGGAACT
TGGCCGGCAGGTTGTTGCCACCCAGATCCGACGGCTTGCCGTTGCCGCAGTACACCCAGACGCGGGTGTT
GTTGGCGATCAGCTTCCCGACGTTCAACAGCGGGTCGTTGCGCTGCCACGCCGGGTCCTCCTTCGGGCCC
CACATGTCGGAGGCCTTGTAGCCGCCAGCGTCACCCATCGCCAGGCCGATCAGGGTGGGACCCATCGCCT
GGGAGGGGTCCAACAGGCCCGACATCGCTCCCGCGTAGACGAACTGCTGGGGGTGATAGATCGCCAGCGT
CAGCGCCGAAGAAGCAGCCATCGAAAGACCGACGACGGCGCTTCCGGTGGGCTTGACGTGCCTGTTGGCC
TGCAGCCACCCCGGCAGCTCGCTGGTCAGGAAGGTCTCCCACTTGTAAGTCTGGCAACCGGCCTTGCCGC
AGGCGGGCTGGTACCAGTCGGAGTAGAAGCTTGACTGGCCACCCACCGGCATGACCACCGACAGGCCCGA
CTGGTCGTACCACTCGAACGCCGGGGTGTTGATGTCCCAGCCGCTGAAGTCGTCCTGCGCGCGCAGGCCG
TCGAGCAGGTACAGGGCGGGCGAGTTGGCACCACCACTTTGGAATTGGACCTTGATGTCACGGCCCATCG
ACGGCGACGGCACCTGCAGGTACTCCACCGGCAAGCCCGGCCGGGAAAATGCCCCCGCGGTCGCCGTGCC
ACCGACGGCGCCGACCAGACCCGACACTAGGGCCGCGCCGACGGCCCCGACCACGAGTCGACGCGACATA
CCCGTGACGGCGCCACGAACCCTGTCAACAAGCTGCA

Protein Sequence : Show Sequence

>NP_218321.1 diacylglycerol acyltransferase/mycolyltransferase Ag85A [Mycobacterium tuberculosis H37Rv]
MQLVDRVRGAVTGMSRRLVVGAVGAALVSGLVGAVGGTATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQ
SGGANSPALYLLDGLRAQDDFSGWDINTPAFEWYDQSGLSVVMPVGGQSSFYSDWYQPACGKAGCQTYKW
ETFLTSELPGWLQANRHVKPTGSAVVGLSMAASSALTLAIYHPQQFVYAGAMSGLLDPSQAMGPTLIGLA
MGDAGGYKASDMWGPKEDPAWQRNDPLLNVGKLIANNTRVWVYCGNGKPSDLGGNNLPAKFLEGFVRTSN
IKFQDAYNAGGGHNGVFDFPDSGTHSWEYWGAQLNAMKPDLQRALGATPNTGPAPQGA

Molecule Role : Protective antigen
Molecule Role Annotation : Researchers have developed DNA vaccines expressing M. tuberculosis antigen 85A (Ag85A, fbpA) and cytokine granulocyte macrophage colony stimulating factor (GM-CSF). The immune strategy of BCG-prime and DNA vaccine boost induced mice to generate efficient immune protection against M. tuberculosis challenge (Dou et al., 2010).
Related Vaccine(s): DNA vaccine co-expressing A85A and caspase-3 , M. tuberculosis DNA vaccine DNAAg85A encoding a single immunogenic M.tb Ag , M. tuberculosis DNA vaccine pcDNA3.1+/Ag85A DNA encoding Ag85A , MVA/IL-15/5Mtb vaccine , MVA85A prime and BCG boost , rBCG-Ag85A/ Ag85B , rBCG-Ag85A[Tokyo]

12. FbpD

Gene Name : FbpD
Sequence Strain (Species/Organism) : Mycobacterium bovis BCG str. Tokyo 172
VO ID : VO_0012366
NCBI Gene ID : 7562680
NCBI Protein GI : 224992215
Locus Tag : JTY_3867
Genbank Accession : AP010918
Protein Accession : YP_002646905
3D structure: PDB ID : 1R88
Taxonomy ID : 561275
Gene Starting Position : 4234158
Gene Ending Position : 4235057
Gene Strand (Orientation) : -
Protein Name : MPT51/MPB51 antigen protein
Protein pI : 6.63
Protein Weight : 29500.66
Protein Length : 299

DNA Sequence : Show Sequence

>NC_012207.1:4234158-4235057 Mycobacterium bovis BCG str. Tokyo 172 DNA, complete genome
CTTAGCGGATCGCACCGACGATATCGCCCGACATAGCGCCCAGCTGGGGCGCCCACGAGCCCCAGCCGTT
GTCACCGCTGGCTGGGAAGTCGAAGTGTCCGTTGTGCCCGCCGACGCTGCGATACTGGTTGTAGAACATG
CGGCTGTTACCCATCGCCTCGGCGGCTTGGCCGATCATGGCGGCGGGATCGCTGGCTCCCGGGTTGGTCG
GGCTCCACACCCACACCCGGGTGTTGTTTTGCGCCAGCAGGCTGGCATGCACCCACGGGTCGTGCCACTT
CCACCGACCCAGCTGTGGTGCTCCCCACATTCCGTTGGTGTCCACACCGCCGAATTGCTGCATGCCCGCC
GCGATCGCACCGTTGGTGGTGGTGTTCGACGGGTACAAAAAGCCCGACATCGAGCCAGCGAAGCCGAAGC
GGTCGGGGTGGAAGGCCGCCAGCGCCATCGCCCCGTAACCGCCCTGAGCGGCGCCAACGGCCGCATGGCC
ACCGGGGGCCAAGCCCCGGTTAGCGGCCAGCCAGTCGGGCAGCTCAGCGGACAAGAAGGTGTCCCACTGC
TTGCTGCCATCCTGCTCCCAGTTGGTGTACATGCTGTACGCACCACCGGCCGGTGCCACCACCGAAATCC
CCTTGCCCGCCAACGTGTTCATCGCGTTACCCGCGGTGACCCAGTTACTGACATCCGGGCCGGCGTTGAA
GGCGTCCAGCAGATACACCGCGTGCGGCCCACCGGCTAGGAAGGCCACCGGGATGTCCCGGCCCATCGAG
GGCGACGGCACCATCAGGTTCTCGTATGGGGCGGCCTTGGCGGTGGGTTCCGCGGCTACCGCGACACCGC
CCAACCCGAATGACAGTGCGGCAATCCAGAGCGCCCGCAGCAGCGCCGACCGACCCTTCA

Protein Sequence : Show Sequence

>YP_002646905.1 MPT51/MPB51 antigen protein [Mycobacterium bovis BCG str. Tokyo 172]
MKGRSALLRALWIAALSFGLGGVAVAAEPTAKAAPYENLMVPSPSMGRDIPVAFLAGGPHAVYLLDAFNA
GPDVSNWVTAGNAMNTLAGKGISVVAPAGGAYSMYTNWEQDGSKQWDTFLSAELPDWLAANRGLAPGGHA
AVGAAQGGYGAMALAAFHPDRFGFAGSMSGFLYPSNTTTNGAIAAGMQQFGGVDTNGMWGAPQLGRWKWH
DPWVHASLLAQNNTRVWVWSPTNPGASDPAAMIGQAAEAMGNSRMFYNQYRSVGGHNGHFDFPASGDNGW
GSWAPQLGAMSGDIVGAIR

Molecule Role : Protective antigen
Molecule Role Annotation : Intravenous vaccination with recombinant bacteria carrying expression plasmid for MPB/MPT51 in BALB/c mice elicited significant protective responses, comparable to those evoked by a live Mycobacterium bovis BCG vaccine (Miki et al., 2004).

13. HBHA

Gene Name : HBHA
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Ra
VO ID : VO_0012373
NCBI Gene ID : 5211814
NCBI Protein GI : 148660242
Locus Tag : MRA_0482
Genbank Accession : CP000611
Protein Accession : YP_001281765
Taxonomy ID : 25177
Gene Starting Position : 567106
Gene Ending Position : 567705
Gene Strand (Orientation) : +
Protein Name : iron-regulated heparin binding hemagglutinin Hbha
Protein pI : 9.84
Protein Weight : 20541.79
Protein Length : 199

DNA Sequence : Show Sequence

>NC_009525.1:567106-567705 Mycobacterium tuberculosis H37Ra, complete genome
CATGGCTGAAAACTCGAACATTGATGACATCAAGGCTCCGTTGCTTGCCGCGCTTGGAGCGGCCGACCTG
GCCTTGGCCACTGTCAACGAGTTGATCACGAACCTGCGTGAGCGTGCGGAGGAGACTCGTACGGACACCC
GCAGCCGGGTCGAGGAGAGCCGTGCTCGCCTGACCAAGCTGCAGGAAGATCTGCCCGAGCAGCTCACCGA
GCTGCGTGAGAAGTTCACCGCCGAGGAGCTGCGTAAGGCCGCCGAGGGCTACCTCGAGGCCGCGACTAGC
CGGTACAACGAGCTGGTCGAGCGCGGTGAGGCCGCTCTAGAGCGGCTGCGCAGCCAGCAGAGCTTCGAGG
AAGTGTCGGCGCGCGCCGAAGGCTACGTGGACCAGGCGGTGGAGTTGACCCAGGAGGCGTTGGGTACGGT
CGCATCGCAGACCCGCGCGGTCGGTGAGCGTGCCGCCAAGCTGGTCGGCATCGAGCTGCCTAAGAAGGCT
GCTCCGGCCAAGAAGGCCGCTCCGGCCAAGAAGGCCGCTCCGGCCAAGAAGGCGGCGGCCAAGAAGGCGC
CCGCGAAGAAGGCGGCGGCCAAGAAGGTCACCCAGAAGTA

Protein Sequence : Show Sequence

>YP_001281765.1 iron-regulated heparin binding hemagglutinin Hbha [Mycobacterium tuberculosis H37Ra]
MAENSNIDDIKAPLLAALGAADLALATVNELITNLRERAEETRTDTRSRVEESRARLTKLQEDLPEQLTE
LREKFTAEELRKAAEGYLEAATSRYNELVERGEAALERLRSQQSFEEVSARAEGYVDQAVELTQEALGTV
ASQTRAVGERAAKLVGIELPKKAAPAKKAAPAKKAAPAKKAAAKKAPAKKAAAKKVTQK

Molecule Role : Protective antigen
Molecule Role Annotation : Purified preparations of HBHA were emulsified in a dimethyldioctadecylammonium bromide-monophosphoryl lipid A adjuvant and tested for the ability to reduce M. tuberculosis infection in the mouse aerosol challenge model for tuberculosis. The HBHA-containing vaccine gave a approximately 0.7-log reduction in CFU in both mouse lungs and spleens compared to adjuvant controls 28 days following challenge (Parra et al., 2004).
Related Vaccine(s): M. tuberculosis HBHA Protein Vaccine

14. KatG

Gene Name : KatG
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0012369
NCBI Gene ID : 885638
NCBI Protein GI : 15609045
Locus Tag : Rv1908c
Genbank Accession : AL123456
Protein Accession : NP_216424
3D structure: PDB ID : 1SJ2
Taxonomy ID : 83332
Gene Starting Position : 2153888
Gene Ending Position : 2156110
Gene Strand (Orientation) : -
Protein Name : catalase-peroxidase
Protein pI : 4.87
Protein Weight : 75544.06
Protein Length : 740
Protein Note : Rv1908c, (MTCY180.10), len: 740 aa. katG, catalase-peroxidase-peroxynitritase T (EC 1.11.1.6) (see citations below), HPI. FASTA results: Q57215 CATALASE-PEROXIDASE from Mycobacterium tuberculosis (740 aa) opt: 5081, E(): 0, (100% identity in 740 aa overlap). Contains peroxidases active site signature (PS00436) and ATP/GTP-binding site motif A (P-loop; PS00017). Cosmid sequence was corrected to agree with a sequencing read from the H37Rv genome. DELETIONS OR DEFECTS IN KATG GENE CAUSE ISONIAZID (INH) RESISTANCE. BELONGS TO THE PEROXIDASE FAMILY. BACTERIAL PEROXIDASE/CATALASE SUBFAMILY. KATG TRANSCRIPTION SEEMS TO BE REGULATED BY FURA|Rv1909c PRODUCT. The catalase-peroxidase activity is associated with the amino-terminal domain but no definite function has been assigned to the carboxy-terminal domain. TBparse score is 0.923.

DNA Sequence : Show Sequence

>NC_000962.3:2153888-2156110 Mycobacterium tuberculosis H37Rv, complete genome
ATCAGCGCACGTCGAACCTGTCGAGGTTCATCACCTTGTCCCAGGCAGCGACGAAGTCCTGCACGAACTT
CGGCTGCGCGTCATCGGCGCCATAGACCTCGACAAGCGCCCGCAACTCCGAGTTGGACCCGAAGACCAGG
TCCACGCGGCTGCCGGTCCACTTCACCTTGCCACTGCCATCCTTGCCCTGGTAGGTCCCGTCATCTGCTG
GCGAGGGCTCCCAGGTGATACCCATGTCGAGCAGGTTCACGAAGAAGTCGTTGGTCAGTGACTCGGAGGC
CTCGGTGAACACGCCCAGCGGTAAGCGCTTGTAGTTTGCGCCGAGGACGCGCAGGCCACCTACCAGCACC
GTCATCTCAGGGGCACTGAGCGTAAGCAGGTTCGCCTTGTCGAGCAGCATGTACTCGGCCGGCAACGGGT
TGCCCTTTCCGAGGTAGTTTCGGAAGCCATCTGCCTTGGGCTCCAGCACGGCAAAGGATTCCACGTCGGT
TTGTTCCTGCGACGCATCCGTGCGGCCCGGGGTGAAGGGCACCGTGATGTTGTGGCCAGCCGCCTTTGCT
GCTTTCTCTATGGCGGCACAGCCACCGAGCACGACGAGGTCGGCGAAGGACACTTTGATGTTCCCCGGCG
CCGCGGAGTTGAATGACTCCTGGATCTCTTCCAGGGTGCGAATGACCTTGCGCAGATCCCCGTCGGGGTC
GTTGACCTCCCACCCGACTTGTGGCTGCAGGCGGATGCGACCACCGTTGGCGCCGCCGCGCTTGTCGCTA
CCACGGAACGACGACGCCGCCGCCCATGCGGTCGAAACTAGCTGTGAGACAGTCAATCCCGATGCCCGGA
TCTGGCTCTTAAGGCTGGCAATCTCGGCTTCGCCGACGAGGTCGTGGCTGACCGCAGGGACCGGATCCTG
CCACAGCAGGGTCTGCTTGGGGACCAGCGGCCCAAGGTATCTCGCAACGGGACCCATGTCTCGGTGGATC
AGCTTGTACCAGGCCTTGGCGAACTCGTCGGCCAATTCCTCGGGGTGTTCCAGCCAGCGACGCGTGATCC
GCTCATAGATCGGATCCACCCGCAGCGAGAGGTCAGTGGCCAGCATCGTCGGGGAGCGCCCTGGCCCGCC
GAACGGGTCCGGGATGGTGCCGGCACCGGCGCCGTCCTTGGCGGTGTATTGCCAAGCGCCAGCAGGGCTC
TTCGTCAGCTCCCACTCGTAGCCGTACAGGATCTCGAGGAAACTGTTGTCCCATTTCGTCGGGGTGTTCG
TCCATACGACCTCGATGCCGCTGGTGATCGCGTCCTTACCGGTTCCGGTGCCATACGAGCTCTTCCAGCC
CAAGCCCATCTGCTCCAGCGGAGCAGCCTCGGGTTCGGGGCCGACCAGATCGGCCGGGCCGGCGCCATGG
GTCTTACCGAAAGTGTGACCGCCGACGATCAGCGCCGCTGTTTCGACGTCGTTCATGGCCATGCGCCGAA
ACGTCTCGCGAATGTCGACCGCCGCGGCCATGGGGTCCGGGTTGCCGTTCGGCCCCTCCGGGTTCACGTA
GATCAGCCCCATCTGCACCGCGGCCAGCGGGTTCTCCAGATCCCGCTTACCGCTGTAACGCTCATCGCCG
AGCCAGGTGGCTTCCTTGCCCCAATAGACCTCATCGGGCTCCCACTGGTCGACCCGGCCGAAGCCGAACC
CGAACGTCTTGAAGCCCATCGATTCCAGCGCGCAGTTGCCGGCGAAAACAATCAGGTCCGCCCATGAGAG
CTTCTTGCCGTACTTCTTCTTGACCGGCCACAGCAGCCGGCGCGCCTTGTCCAAGCTGGCGTTGTCGGGC
CAGCTGTTAAGCGGCGCGAACCGCTGCATGCCGCCCCCGGCGCCGCCGCGGCCGTCGTGGATGCGGTAGG
TGCCGGCAGCGTGCCACGCCATCCGGATAAACAGCGGCCCGTAGTGGCCGTAGTCGGCGGGCCACCACGG
CTGCGAGGTGGTCATCACTTCCTCGATGTCCCGCGTCAGGGCGTCAACGTCGATGGTCGCGACCTCCGCG
GCATAGTCGAACGCCGCACCCATCGGGTCAGCGACGGCCGGGTTTTGGTGCAGTACCTTCAGATTGAGCC
GGTTGGGCCACCAGTCCTGGTTTCCGCCGCCCTCGACGGGGTATTTCATATGACCCACGACGGGACAGCC
GTTGCTAGCGGCTCCGGTGGTGGTTTCTGTAATGGGTGGGTGTTGCTCGGGCA

Protein Sequence : Show Sequence

>NP_216424.1 catalase-peroxidase [Mycobacterium tuberculosis H37Rv]
MPEQHPPITETTTGAASNGCPVVGHMKYPVEGGGNQDWWPNRLNLKVLHQNPAVADPMGAAFDYAAEVAT
IDVDALTRDIEEVMTTSQPWWPADYGHYGPLFIRMAWHAAGTYRIHDGRGGAGGGMQRFAPLNSWPDNAS
LDKARRLLWPVKKKYGKKLSWADLIVFAGNCALESMGFKTFGFGFGRVDQWEPDEVYWGKEATWLGDERY
SGKRDLENPLAAVQMGLIYVNPEGPNGNPDPMAAAVDIRETFRRMAMNDVETAALIVGGHTFGKTHGAGP
ADLVGPEPEAAPLEQMGLGWKSSYGTGTGKDAITSGIEVVWTNTPTKWDNSFLEILYGYEWELTKSPAGA
WQYTAKDGAGAGTIPDPFGGPGRSPTMLATDLSLRVDPIYERITRRWLEHPEELADEFAKAWYKLIHRDM
GPVARYLGPLVPKQTLLWQDPVPAVSHDLVGEAEIASLKSQIRASGLTVSQLVSTAWAAASSFRGSDKRG
GANGGRIRLQPQVGWEVNDPDGDLRKVIRTLEEIQESFNSAAPGNIKVSFADLVVLGGCAAIEKAAKAAG
HNITVPFTPGRTDASQEQTDVESFAVLEPKADGFRNYLGKGNPLPAEYMLLDKANLLTLSAPEMTVLVGG
LRVLGANYKRLPLGVFTEASESLTNDFFVNLLDMGITWEPSPADDGTYQGKDGSGKVKWTGSRVDLVFGS
NSELRALVEVYGADDAQPKFVQDFVAAWDKVMNLDRFDVR

Molecule Role : Protective antigen
Molecule Role Annotation : DNA vaccines encoding KatG antigen fused to TPA signal sequences evoked a significant protective response in mice aerogenically challenged with low doses of Mycobacterium tuberculosis Erdman 17 to 21 days after the final immunization (Li et al., 1999).
Related Vaccine(s): M. tuberculosis DNA vaccine (containing the ESAT-6, MPT-64, MPT-83, and KatG constructs) , M. tuberculosis DNA Vaccine encoding KatG

15. leuD

Gene Name : leuD
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 888225
NCBI Protein GI : 15610124
Locus Tag : Rv2987c
Genbank Accession : BX842581
Protein Accession : NP_217503
3D structure: PDB ID : 3H5J
Taxonomy ID : 83332
Gene Starting Position : 3344032
Gene Ending Position : 3344628
Gene Strand (Orientation) : -
Protein Name : isopropylmalate isomerase small subunit
Protein pI : 4.89
Protein Weight : 20390.6
Protein Length : 198
Protein Note : catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate in leucine biosynthesis; forms a heterodimer of LeuC/D

DNA Sequence : Show Sequence

>gi|57116681:3344032-3344628 Mycobacterium tuberculosis H37Rv, complete genome
CTCAGGGGGCGGGTAGAGTGCGCGGTTTCCAGTACGCGCACGCACCCTCAAAGGCCTCGATCTCGTCGAG
TTTCCGCAGCGTAAGGGCTATATCGTCGAGACCTTCAAGCAGCCGCCACGCCGAGTGGTCGTCAATCTTG
AACGGCAGCACCACTGTTGCTGCGGTGATAATTCGATCTTGAAGATTGGCAGTGATTTCCAGGCCCGGAC
TCTGCTCAATGAGCTTCCACAGGAGTTCCACATCGTCTTGGGCAACCTCGGCCGCCAGCAGCCCGGCCTT
GCCCGCGTTGCCGCGGAAAATGTCACCAAATCGGGATGAGATAACCACCCGGAATCCGTAGTCCATGAGC
GCCCAGACCGCATGCTCTCGCGAGGATCCGGTGCCGAAATCGGGCCCGGCAACCAGGACCGAACCCCGGT
CAAAGGGACTGAGGTTTAGCACGAATGCAGGATCCGACCGCCAACCCGCGAACAAGCCGTCCTCGAAACC
GGTTCGGGTGACCCGCTTCAGAAAGACCGCGGGAATGATCTGATCGGTGTCGACATTGGACCGCCGCAAC
GGCACGCCAATACCAGAGTGGGTGTGAAAGGCTTCCA

Protein Sequence : Show Sequence

>gi|15610124|ref|NP_217503.1| isopropylmalate isomerase small subunit [Mycobacterium tuberculosis H37Rv]
MEAFHTHSGIGVPLRRSNVDTDQIIPAVFLKRVTRTGFEDGLFAGWRSDPAFVLNLSPFDRGSVLVAGPD
FGTGSSREHAVWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVAQDDVELLWKLIEQSPGLEITANLQ
DRIITAATVVLPFKIDDHSAWRLLEGLDDIALTLRKLDEIEAFEGACAYWKPRTLPAP

Molecule Role : Virmugen
Molecule Role Annotation : Deletion of leuD gene was shown to be highly attenuated. Immunocompetent BALB/c mice vaccinated with the DleuD auxotroph were protected as well as BCG in terms of survival following an intravenous or aerosol challenge with virulent M. tuberculosis (Hondalus et al., 2000).
Related Vaccine(s): Mycobacterium tuberculosis leuD mutant vaccine

16. lysA

Gene Name : lysA
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 886960
NCBI Protein GI : 15608433
Locus Tag : Rv1293
Genbank Accession : BX842576
Protein Accession : NP_215809
3D structure: PDB ID : 1HKW
Taxonomy ID : 83332
Gene Starting Position : 1448027
Gene Ending Position : 1449370
Gene Strand (Orientation) : ?
Protein Name : diaminopimelate decarboxylase LysA
Protein pI : 5.07
Protein Weight : 42101.78
Protein Length : 447
Protein Note : Rv1293, (MTCY373.13), len: 447 aa. Probable lysA, diaminopimelate decarboxylase (EC 4.1.1.20) (see citation below), almost identical to DCDA_MYCTU|P31848. Contains PS00878 Orn/DAP/Arg decarboxylases family 2 pyridoxal-P attachment site, PS00879 Orn/DAP/Arg decarboxylases family 2 signature 2. BELONGS TO FAMILY 2 OF ORNITHINE, DAP, AND ARGININE DECARBOXYLASES.

DNA Sequence : Show Sequence

>gi|57116681:1448027-1449370 Mycobacterium tuberculosis H37Rv, complete genome
TGTGAACGAGCTGCTGCACTTAGCGCCGAATGTGTGGCCGCGCAATACTACTCGCGATGAAGTCGGTGTG
GTCTGCATCGCAGGAATTCCACTGACGCAGCTCGCCCAGGAGTACGGGACCCCGCTGTTCGTCATCGACG
AGGACGACTTTCGCTCGCGCTGCCGAGAAACCGCCGCGGCCTTTGGAAGTGGGGCGAACGTGCACTATGC
CGCCAAGGCGTTCCTGTGCAGCGAAGTAGCCCGGTGGATCAGCGAAGAAGGGCTCTGTCTGGACGTTTGC
ACCGGTGGGGAGTTGGCGGTCGCGCTGCACGCTAGCTTTCCGCCCGAGCGAATTACCTTGCACGGCAACA
ACAAATCGGTCTCAGAGTTGACCGCTGCGGTCAAAGCCGGAGTCGGCCATATTGTCGTCGATTCGATGAC
CGAGATCGAGCGCCTCGACGCCATCGCGGGCGAGGCCGGAATCGTCCAGGATGTCCTGGTGCGTCTCACC
GTCGGTGTCGAGGCGCACACCCACGAGTTCATCTCCACCGCGCACGAGGACCAGAAATTCGGGTTATCGG
TGGCCAGCGGCGCGGCCATGGCAGCGGTGCGGCGCGTTTTCGCCACTGATCACCTGCGCCTGGTTGGGCT
ACACAGCCACATCGGTTCGCAGATCTTCGACGTGGACGGCTTCGAACTCGCCGCGCACCGTGTCATCGGC
CTGCTACGCGACGTCGTCGGCGAGTTCGGTCCCGAAAAGACGGCACAGATCGCGACCGTCGATCTCGGTG
GCGGCTTGGGCATCTCGTATTTGCCGTCCGACGACCCACCGCCGATAGCCGAGCTCGCGGCCAAGCTGGG
TACCATCGTGAGCGACGAGTCAACGGCCGTGGGGCTGCCGACGCCCAAGCTCGTTGTGGAGCCCGGACGC
GCCATCGCCGGACCGGGCACCATCACGTTGTATGAGGTCGGCACCGTTAAGGACGTCGATGTCAGCGCCA
CAGCGCATCGACGTTACGTCAGTGTCGACGGCGGCATGAGCGACAACATCCGCACCGCGCTCTACGGCGC
GCAGTATGACGTCCGGCTGGTGTCTCGAGTCAGCGACGCCCCGCCGGTACCGGCCCGTCTGGTCGGAAAG
CACTGCGAAAGTGGCGATATCATCGTGCGGGACACCTGGGTGCCCGACGATATTCGGCCCGGCGATCTGG
TTGCGGTTGCCGCCACCGGCGCTTACTGCTATTCGCTGTCGAGTCGTTACAACATGGTCGGCCGTCCCGC
TGTGGTAGCGGTGCACGCGGGCAACGCTCGCCTGGTCCTGCGTCGGGAGACGGTCGACGATTTGCTGAGT
TTGGAAGTGAGGTG

Protein Sequence : Show Sequence

>gi|15608433|ref|NP_215809.1| diaminopimelate decarboxylase LysA [Mycobacterium tuberculosis H37Rv]
MNELLHLAPNVWPRNTTRDEVGVVCIAGIPLTQLAQEYGTPLFVIDEDDFRSRCRETAAAFGSGANVHYA
AKAFLCSEVARWISEEGLCLDVCTGGELAVALHASFPPERITLHGNNKSVSELTAAVKAGVGHIVVDSMT
EIERLDAIAGEAGIVQDVLVRLTVGVEAHTHEFISTAHEDQKFGLSVASGAAMAAVRRVFATDHLRLVGL
HSHIGSQIFDVDGFELAAHRVIGLLRDVVGEFGPEKTAQIATVDLGGGLGISYLPSDDPPPIAELAAKLG
TIVSDESTAVGLPTPKLVVEPGRAIAGPGTITLYEVGTVKDVDVSATAHRRYVSVDGGMSDNIRTALYGA
QYDVRLVSRVSDAPPVPARLVGKHCESGDIIVRDTWVPDDIRPGDLVAVAATGAYCYSLSSRYNMVGRPA
VVAVHAGNARLVLRRETVDDLLSLEVR

Molecule Role : Virmugen
Molecule Role Annotation : A lysA mutant, in combination with a secA2 mutation, is highly attenuated in mice and induced significant protection from challenge with wild type M. tuberculosis (Hinchey et al., 2011).
Related Vaccine(s): Mycobacterium tuberculosis lysA/secA2 mutant vaccine

17. mce-2

Gene Name : mce-2
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis CDC1551
NCBI Gene ID : 925014
NCBI Protein GI : 15839991
Locus Tag : MT0618
Genbank Accession : AE000516
Protein Accession : NP_335028
Taxonomy ID : 83331
Gene Starting Position : 688269
Gene Ending Position : 689483
Gene Strand (Orientation) : +
Protein Name : virulence factor
Protein pI : 4.96
Protein Weight : 39757.76
Protein Length : 404
Protein Note : identified by similarity to GP:5814111; match to protein family HMM PF02470; match to protein family HMM TIGR00996

DNA Sequence : Show Sequence

>gi|50953765:688269-689483 Mycobacterium tuberculosis CDC1551, complete genome
GGTGCCAACGCTGGTGACGAGGAAGAACCGACGTGCGTGGCTGTATGTGGAGGGTGTTGTCCTGCTGTTG
GTGGGCGCGTTGGTGCTCGTATTGGTGTACAAGCAGTTTCGTGGGGAATTCACGCCGAAGACCGAGCTGA
CTATGGTCGCCTCCCGGGCTGGGCTGGTTATGGAAGCTGGATCCAAAGTCACCTACAACGGGGTGGAGAT
CGGCCGGGTGGGCAGCATTTCGGAGATTGAGCGTGACGGCCGGCCGGCGGCGAAGCTGGTTTTGGACGTG
AATCCTCGCTACATCAGCCTGATTCCGGTCAATGTGGTGGCCGATATCGAGGCGGCCACCCTGTTCGGCA
ACAAGTATGTTGCGCTGTCCGCGCCGAAAATTCCTCAACAGCAGCGGATTTCCTCACATGACGTGATTGA
TGTGGGGTCGGTGACCACCGAATTCAACACGTTGTTCGAGACGATCACCTCGATCGCCGAGAAGGTGGAT
CCGATCGAGCTGAACGCGACGCTGTCCGCGGTAGCACAGGCGCTGGATGGGCTGGGCGGCAAGTTCGGTG
AGTCGATCGTTAATGGCAATCAGATTCTGGCGCAATTAAATCCGCGGCTGCCGCAGCTCGGCTATGATGT
TCGGCGGTTGGCGGATCTCGGTGAGGTCTATGTCGATGCTTCGCCGGATCTGTGGTCCTTTCTGCAGAAC
GCACTGACCACTGCGCGCACATTGACCAGCCAACAGCGCGATCTGGATGCCGCGTTGTTGGCGGCTACGG
GTGCGGGCAACACCGGTGAAGACGTTTTTGCTCGAGGCGGGCCGTATCTTGCGCGCGCAGCCGCCGATCT
GGTGCCCACCGCTACGCTGCTGGACACCTACAGTCCCGAACTGTTCTGCATGATCCGCAACTTTCACGAC
GCTGCGCCCAAAGTCGCGGACGCGGTGGGCGGCAACGGCTATTCGCTAGCGGCCGCCGGAACGATTTTGG
GAGCACCCAATCCCTATGTCTATCCGGACAATCTGCCGCGGGTGAATGCCCACGGTGGACCCGGGGGCCG
ACCGGGCTGCTGGCAGACGATCACCCGGGAGCTGTGGCCGGCACCCTATCTGGTGATGGACACCGGTGCC
AGCCTCGCACCGTACAACCACGTCGAGCTCGGCCAACCGATGTTCACTGAATACGTATGGGGACGCCAAT
ACGGAGAGAACACGATCAACCCATG

Protein Sequence : Show Sequence

>gi|15839991|ref|NP_335028.1| virulence factor [Mycobacterium tuberculosis CDC1551]
MPTLVTRKNRRAWLYVEGVVLLLVGALVLVLVYKQFRGEFTPKTELTMVASRAGLVMEAGSKVTYNGVEI
GRVGSISEIERDGRPAAKLVLDVNPRYISLIPVNVVADIEAATLFGNKYVALSAPKIPQQQRISSHDVID
VGSVTTEFNTLFETITSIAEKVDPIELNATLSAVAQALDGLGGKFGESIVNGNQILAQLNPRLPQLGYDV
RRLADLGEVYVDASPDLWSFLQNALTTARTLTSQQRDLDAALLAATGAGNTGEDVFARGGPYLARAAADL
VPTATLLDTYSPELFCMIRNFHDAAPKVADAVGGNGYSLAAAGTILGAPNPYVYPDNLPRVNAHGGPGGR
PGCWQTITRELWPAPYLVMDTGASLAPYNHVELGQPMFTEYVWGRQYGENTINP

Molecule Role : Virmugen
Molecule Role Annotation : Mce-2 and mce-3 mutants in Mycobacterium tuberculosis is attenuated and provide protection against challenge for mice (Aguilar et al., 2006).
Related Vaccine(s): Mycobacterium tuberculosis mce-2 mutant vaccine

18. mce-3

Gene Name : mce-3
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis CDC1551
NCBI Gene ID : 923491
NCBI Protein GI : 15841441
Locus Tag : MT2018
Genbank Accession : AE000516
Protein Accession : NP_336478
Taxonomy ID : 83331
Gene Starting Position : 2206659
Gene Ending Position : 2207936
Gene Strand (Orientation) : +
Protein Name : virulence factor
Protein pI : 6.51
Protein Weight : 41527.31
Protein Length : 425
Protein Note : identified by similarity to GP:5814111; match to protein family HMM PF02470; match to protein family HMM TIGR00996

DNA Sequence : Show Sequence

>gi|50953765:2206659-2207936 Mycobacterium tuberculosis CDC1551, complete genome
CATGAGACGCGGGCCGGGTCGACACCGTTTGCACGACGCGTGGTGGACGCTGATCCTGTTCGCGGTGATC
GGGGTGGCTGTCCTGGTGACGGCGGTGTCCTTCACGGGCAGCTTGCGGTCGACTGTGCCGGTGACGCTGG
CGGCCGACCGCTCCGGGCTGGTGATGGACTCCGGCGCCAAGGTCATGATGCGCGGTGTGCAGGTCGGCCG
GGTCGCCCAGATCGGTCGGATCGAGTGGGCCCAGAACGGGGCGAGCCTCAGACTGGAGATCGACCCCGAC
CAGATCCGGTACATCCCGGCCAATGTCGAGGCACAGATCAGCGCCACCACCGCATTCGGTGCCAAGTTCG
TCGACCTGGTGATGCCGCAAAACCCAAGTCGTGCACGGCTGTCCGCTGGGGCGGTACTGCATTCGAAGAA
CGTCAGCACGGAAATCAACACCGTCTTCGAAAACGTCGTCGACCTGCTCAACATGATCGACCCGCTGAAA
CTGAACGCCGTGCTGACCGCGGTCGCCGACGCCGTTCGCGGGCAAGGTGAACGGATAGGCCAGGCCACCA
CCGACCTCAACGAGGTGCTGGAGGCACTCAACGCACGCGGCGACACCATCGGCGGCAACTGGCGATCGCT
CAAGAACTTCACCGACACCTATGACGCGGCCGCCCAAGACATCCTGACGATCCTGAACGCCGCCAGCACC
ACCAGTGCGACCGTCGTGAATCATTCGACGCAGCTGGATGCCTTGCTACTCAACGCCATCGGACTATCCA
ACGCTGGCACCAACCTGCTTGGCAGCAGCCGAGACAATCTCGTCGGCGCGGCCGACATCCTGGCGCCGAC
CACGAGCCTGCTGTTCAAGTACAACCCCGAATACACCTGCTTCCTGCAGGGCGCCAAGTGGTATCTCGAC
AACGGCGGCTATGCGGCCTGGGGCGGGGCCGACGGGCGCACGCTACAACTCGATGTGGCGCTACTGTTCG
GCAACGACCCCTATGTCTATCCGGACAACCTGCCGGTTGTCGCGGCCAAGGGGGGTCCCGGCGGAAGGCC
GGGATGCGGGCCATTGCCGGATGCCACCCACAACTTCCCGGTGCGCCAGCTGGTCACCAACACCGGATGG
GGAACCGGGCTGGACATCCGGCCCAACCCCGGCATCGGGCATCCCTGCTGGGCCAACTACTTCCCGGTGA
CCCGCGCGGTGCCCGAGCCGCCGTCGATCCGTCAGTGCATCCCCGGGCCGGCGATCGGGCCCAACCCCGC
GGCGGGGGAGCAGCCATG

Protein Sequence : Show Sequence

>gi|15841441|ref|NP_336478.1| virulence factor [Mycobacterium tuberculosis CDC1551]
MRRGPGRHRLHDAWWTLILFAVIGVAVLVTAVSFTGSLRSTVPVTLAADRSGLVMDSGAKVMMRGVQVGR
VAQIGRIEWAQNGASLRLEIDPDQIRYIPANVEAQISATTAFGAKFVDLVMPQNPSRARLSAGAVLHSKN
VSTEINTVFENVVDLLNMIDPLKLNAVLTAVADAVRGQGERIGQATTDLNEVLEALNARGDTIGGNWRSL
KNFTDTYDAAAQDILTILNAASTTSATVVNHSTQLDALLLNAIGLSNAGTNLLGSSRDNLVGAADILAPT
TSLLFKYNPEYTCFLQGAKWYLDNGGYAAWGGADGRTLQLDVALLFGNDPYVYPDNLPVVAAKGGPGGRP
GCGPLPDATHNFPVRQLVTNTGWGTGLDIRPNPGIGHPCWANYFPVTRAVPEPPSIRQCIPGPAIGPNPA
AGEQP

Molecule Role : Virmugen
Molecule Role Annotation : Mce-2 and mce-3 mutants in Mycobacterium tuberculosis is attenuated and provide protection against challenge for mice (Aguilar et al., 2006).
Related Vaccine(s): Mycobacterium tuberculosis mce-2 mutant vaccine , Mycobacterium tuberculosis mce-3 mutant vaccine

19. Mpt63

Gene Name : Mpt63
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0011176
NCBI Gene ID : 885334
NCBI Protein GI : 15609063
Locus Tag : Rv1926c
Genbank Accession : AL123456
Protein Accession : NP_216442
3D structure: PDB ID : 1LMI
Taxonomy ID : 83332
Gene Starting Position : 2178956
Gene Ending Position : 2179435
Gene Strand (Orientation) : -
Protein Name : immunogenic protein Mpt63
Protein pI : 4.67
Protein Weight : 14827.76
Protein Length : 159
Protein Note : Rv1926c, (MT1977, MTCY09F9.38), len: 159 aa. mpt63 (alternate gene name: mpb63), immunogenic protein (see citations below), identical to MPT63|MPB63 from Mycobacterium bovis (159 aa). Exported protein containing a N-terminal signal sequence: see notes below about proteomics.; mpb63

DNA Sequence : Show Sequence

>NC_000962.3:2178956-2179435 Mycobacterium tuberculosis H37Rv, complete genome
TCTACGGCTCCCAAATCAGCAGATCCTCCATGCCGTTGTTCATCGCGACGATGGTTGGCGATGGGCCGGT
GACATCGAAGTAGATTTTGCCGGTCGATTGTTCGCCTTGGGGGATAGTGGCTCCGCTAATGGTGTCGGGG
CCCGCGGCTTGCCACAGCACCCGGTAGTTGATGCCGTCGGCGGTGCGGGCATTGAACTGCGAGACCGCGG
GCGTGACGCTGCCGCGAATCGCATTGACCGTGGCAGTGGCCTCCCAGACCTGGCCGGCCACCGGATAGCC
GGGGATGACTGCCGTGCTGGATTTGAGATCACTGACCTTCCAGCCGAGCACGACTTGGCCAACGGTGTCG
GTCATCGTTAGCTCACTGCCAAGTTTTCCGGTGATGGGATAGGCAGCCAACGCGACCGGTGCCGCAAAGG
TCGCGATGGCCGCCATGGCCACGACCGCTACTGCCGTCTTGATCATTGTGGTGAGCTTCA

Protein Sequence : Show Sequence

>NP_216442.1 immunogenic protein Mpt63 [Mycobacterium tuberculosis H37Rv]
MKLTTMIKTAVAVVAMAAIATFAAPVALAAYPITGKLGSELTMTDTVGQVVLGWKVSDLKSSTAVIPGYP
VAGQVWEATATVNAIRGSVTPAVSQFNARTADGINYRVLWQAAGPDTISGATIPQGEQSTGKIYFDVTGP
SPTIVAMNNGMEDLLIWEP

Molecule Role : Protective antigen
Molecule Role Annotation : MPT63 (Rv1926c), a major secreted protein of Mycobacterium tuberculosis, is immunoreactive in antibody assays in humans and animals and provides protection as a combined DNA vaccine in mice (Mustafa, 2009).

20. MPT64

Gene Name : MPT64
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0012367
NCBI Gene ID : 885925
NCBI Protein GI : 15609117
Locus Tag : Rv1980c
Genbank Accession : A42312
Protein Accession : NP_216496
3D structure: PDB ID : 2HHI
Taxonomy ID : 83332
Gene Starting Position : 2223342
Gene Ending Position : 2224028
Gene Strand (Orientation) : -
Protein Name : immunogenic protein Mpt64
Protein pI : 4.6
Protein Weight : 23267.4
Protein Length : 228
Protein Note : Rv1980c, (MT2032, MTCY39.39), len: 228 aa. mpt64 (alternate gene name: mpb64), immunogenic protein (alternate gene name: mpb64) (see citations below), identical to MPT64|MPB64 from Mycobacterium bovis (228 aa). Similar to Rv3036c|MTV012.51c from Mycobacterium tuberculosis. Exported protein containing a N-terminal signal sequence: see notes below about proteomics.; mpb64

DNA Sequence : Show Sequence

>NC_000962.3:2223342-2224028 Mycobacterium tuberculosis H37Rv, complete genome
TCTAGGCCAGCATCGAGTCGATCGCGGAACGTGGGACCAATACCTGGGTTGGGCCGGCTGCTTCGGGCAG
CAACTCCCCCGGGTTGAAGAAGAAAATCACCCCGTCGTTCGTGACTGCGAAGTTCTGATAATTCACCGGG
TCCAAGCCGGCATTCGGCGCTATCGATACCTGTTGTCCGGTCTGCTTGCTCAGTTCACCTTGCACAATGG
GGAAGACGACTGGCAGCGGATCGGTGTCAGCCTGCCACAGCGTGTCATAGGTGATTGGCTTGCGATAGGC
CTGGTCCCAATCGAAGGCCTTGTACGTGGTCGTTGGGTGCGTGCCGCCGGCGTTCTGGTAGACCTTGAGC
ACCACGGCCTGCGTACCACGCGGCGGTATCGCGGACTGGTATGTGGCCGAGGTGATATTCAATTCGTAGG
GGGCTTCGCGTGGAGTGGACGATGTGGCCGCGCTGAGGAACTTGTCGCGCGTCTGGGCGATGTAATTTTC
CAGCGACTTCTGGTCGGGGTAGTAACTGGGCAGGCTGATGTTGATGTTGTAGGCCGGGTCGGACATTTGA
ATCTGGCACGCCTGGCCGGTATCGGTGCCTTTCAACTCCTCGCAGTAGGTCTTGGGCGCGGCCGTGGCCA
CACCCGAACAACAGAGCAAAACGACAGCCGTGACCAGCATGAAGATCTTGATGCGCA

Protein Sequence : Show Sequence

>NP_216496.1 immunogenic protein Mpt64 [Mycobacterium tuberculosis H37Rv]
MRIKIFMLVTAVVLLCCSGVATAAPKTYCEELKGTDTGQACQIQMSDPAYNINISLPSYYPDQKSLENYI
AQTRDKFLSAATSSTPREAPYELNITSATYQSAIPPRGTQAVVLKVYQNAGGTHPTTTYKAFDWDQAYRK
PITYDTLWQADTDPLPVVFPIVQGELSKQTGQQVSIAPNAGLDPVNYQNFAVTNDGVIFFFNPGELLPEA
AGPTQVLVPRSAIDSMLA

Molecule Role : Protective antigen
Molecule Role Annotation : DNA vaccines encoding secreted proteins Ag85B and MPT64 protect mice from the tuberculosis H37Rv strain (Luo et al., 2004).
*Note* MPT64 in combination with Ag85B was protective, DNA vaccine of just MPT64 was mildly protective in mice.
Related Vaccine(s): M. tuberculosis DNA vaccine (containing the ESAT-6, MPT-64, MPT-83, and KatG constructs) , M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83

21. MPT83

Gene Name : MPT83
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0012409
NCBI Gene ID : 887155
NCBI Protein GI : 15610010
Locus Tag : Rv2873
Genbank Accession : AL123456
Protein Accession : NP_217389
Taxonomy ID : 83332
Gene Starting Position : 3183904
Gene Ending Position : 3184566
Gene Strand (Orientation) : +
Protein Name : cell surface lipoprotein
Protein pI : 4.73
Protein Weight : 20581.44
Protein Length : 220
Protein Note : Rv2873, (MTCY274.04), len: 220 aa. mpt83 (alternate gene name: mpb83), cell surface lipoprotein (see citations below). Also similar to upstream ORF Q50769|MP70_MYCTU|MPT70|MPB70|Rv2875|MT2943|MTCY274.06 which is also known as MAJOR SECRETED IMMUNOGENIC PROTEIN MPT70 PRECURSOR from Mycobacterium tuberculosis (193 aa), FASTA scores: opt: 806, E(): 2.7e-38, (70.25% identity in 185 aa overlap). BELONGS TO THE MPT70 / MPT83 FAMILY. ATTACHED TO THE MEMBRANE BY A LIPID ANCHOR.; mpb83

DNA Sequence : Show Sequence

>NC_000962.3:3183904-3184566 Mycobacterium tuberculosis H37Rv, complete genome
GATGATCAACGTTCAGGCCAAACCGGCCGCAGCAGCGAGCCTCGCAGCCATCGCGATTGCGTTCTTAGCG
GGTTGTTCGAGCACCAAACCCGTGTCGCAAGACACCAGCCCGAAACCGGCGACCAGCCCGGCGGCGCCCG
TTACCACGGCGGCAATGGCTGACCCCGCAGCGGACCTGATTGGTCGTGGGTGCGCGCAATACGCGGCGCA
AAATCCCACCGGTCCCGGATCGGTGGCCGGAATGGCGCAAGACCCGGTCGCTACCGCGGCTTCCAACAAC
CCGATGCTCAGTACCCTGACCTCGGCTCTGTCGGGCAAGCTGAACCCGGATGTGAATCTGGTCGACACCC
TCAACGGCGGCGAGTACACCGTTTTCGCCCCCACCAACGCCGCATTCGACAAGCTGCCGGCGGCCACTAT
CGATCAACTCAAGACTGACGCCAAGCTGCTCAGCAGCATCCTGACCTACCACGTGATAGCCGGCCAGGCG
AGTCCGAGCAGGATCGACGGCACCCATCAGACCCTGCAAGGTGCCGACCTGACGGTGATAGGCGCCCGCG
ACGACCTCATGGTCAACAACGCCGGTTTGGTATGTGGCGGAGTTCACACCGCCAACGCGACGGTGTACAT
GATCGATACGGTGCTGATGCCCCCGGCACAGTA

Protein Sequence : Show Sequence

>NP_217389.1 cell surface lipoprotein [Mycobacterium tuberculosis H37Rv]
MINVQAKPAAAASLAAIAIAFLAGCSSTKPVSQDTSPKPATSPAAPVTTAAMADPAADLIGRGCAQYAAQ
NPTGPGSVAGMAQDPVATAASNNPMLSTLTSALSGKLNPDVNLVDTLNGGEYTVFAPTNAAFDKLPAATI
DQLKTDAKLLSSILTYHVIAGQASPSRIDGTHQTLQGADLTVIGARDDLMVNNAGLVCGGVHTANATVYM
IDTVLMPPAQ

Molecule Role : Protective antigen
Molecule Role Annotation : A single DNA vaccine encoding the MPT-83 tuberculosis antigens evoked partial protection against an aerogenic challenge with M. tuberculosis Erdman in the mouse model of pulmonary tuberculosis (Morris et al., 2000).
Related Vaccine(s): M. tuberculosis DNA vaccine (containing the ESAT-6, MPT-64, MPT-83, and KatG constructs) , M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83

22. Mtb72F

Gene Name : Mtb72F
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis
VO ID : VO_0011173
NCBI Protein GI : 283801990
Taxonomy ID : 32630
Gene Strand (Orientation) : ?
Protein pI : 4.95
Protein Weight : 66088.21
Protein Length : 799
Protein Note : Description of Artificial Sequence:tri-fusion protein MTB72F (Ra12-TbH9-Ra35 or MTB32-MTB39 fusion)

Protein Sequence : Show Sequence

>CBI85484.1 unnamed protein product [synthetic construct]
MHHHHHHTAASDNFQLSQGGQGFAIPIGQAMAIAGQIRSGGGSPTVHIGPTAFLGLGVVDNNGNGARVQR
VVGSAPAASLGISTGDVITAVDGAPINSATAMADALNGHHPGDVISVTWQTKSGGTRTGNVTLAEGPPAE
FMVDFGALPPEINSARMYAGPGSASLVAAAQMWDSVASDLFSAASAFQSVVWGLTVGSWIGSSAGLMVAA
ASPYVAWMSVTAGQAELTAAQVRVAAAAYETAYGLTVPPPVIAENRAELMILIATNLLGQNTPAIAVNEA
EYGEMWAQDAAAMFGYAAATATATATLLPFEEAPEMTSAGGLLEQAAAVEEASDTAAANQLMNNVPQALQ
QLAQPTQGTTPSSKLGGLWKTVSPHRSPISNMVSMANNHMSMTNSGVSMTNTLSSMLKGFAPAAARQAVQ
TAAQNGVRAMSSLGSSLGSSGLGGGVAANLGRAASVGSLSVPQAWAAANQAVTPAARALPLTSLTSAAER
GPGQMLGGLPVGQMGARAGGGLSGVLRVPPRPYVMPHSPAAGDIAPPALSQDRFADFPALPLDPSAMVAQ
VGPQVVNINTKLGYNNAVGAGTGIVIDPNGVVLTNNHVIAGATDINAFSVGSGQTYGVDVVGYDRTQDVA
VLQLRGAGGLPSAAIGGGVAVGEPVVAMGNSGGQGGTPRAVPGRVVALGQTVQASDSLTGAEETLNGLIQ
FDAAIQPGDSGGPVVNGLGQVVGMNTAAS

Molecule Role : Protective antigen
Molecule Role Annotation : Our lead construct, Mtb72F, codes for a 72-kDa polyprotein genetically linked in tandem in the linear order Mtb32(C)-Mtb39-Mtb32(N). All forms of Mtb72F immunization tested resulted in the protection of C57BL/6 mice against aerosol challenge with a virulent strain of M. tuberculosis (Skeiky et al., 2004).
Related Vaccine(s): M. tuberculosis Mtb72F Protein Subunit Vaccine

23. panC

Gene Name : panC
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 885459
NCBI Protein GI : 15610738
Locus Tag : Rv3602c
Genbank Accession : BX842583
Protein Accession : NP_218119
Taxonomy ID : 83332
Gene Starting Position : 4044280
Gene Ending Position : 4045209
Gene Strand (Orientation) : -
Protein Name : pantoate--beta-alanine ligase
Protein pI : 5.96
Protein Weight : 29602.42
Protein Length : 309
Protein Note : catalyzes the formation of (R)-pantothenate from pantoate and beta-alanine

DNA Sequence : Show Sequence

>gi|57116681:4044280-4045209 Mycobacterium tuberculosis H37Rv, complete genome
ATCAGTTTCTCCAATGTGATTCGAGGATTGCCCGGTATCCGTCCGGGCGGTCGGTGCCGGCGAAAGTTCC
GATTTCAATCGCAATGTTGTCCAGCAGCCTGGTGGTGCCAAGCCGGGCAGCAACCAGCAGCCGACCGGAA
CCGTTGAGCGGCATCGGGCCAAGCCCGATATCGCGCAGCTCCAGGTAGTCGACCGCCACGCCGGGTGCAG
CGTCGAGCACCGCACGGGCGGCATCCAGCGCGGCCTGCGCGCCAGCCGTTGCCGCATGCGCTGCGGCCGT
TAGCGCCGCCGAGAGCGCGACGGCCGCCGCACGCTGGGCCGGGTCCAGGTAGCGGTTGCGCGACGACATC
GCCAGCCCGTCGGCTTCGCGCACGGTCGGCACGCCGACCACCGCGACATCGAGGTTGAAGTCCGCGACCA
GCTGCCGGATCAGCACCAGCTGCTGGTAGTCCTTCTCACCGAAGAACACCCGATCCGGGCGCACGATCTG
CAGCAGCTTTAGCACGACCGTCAGCACGCCGGCGAAATGGGTTGGCCGCGGGCCGCCCTCGAGTTCGGCG
GCCAACGGACCGGGTTGCACGGTGGTGCGCAGGCCGTCGGGATACATCGCCGCGGTAGTTGGCGTGAAAG
CGATTTCCACGCCTTCGGCCCGCAGTTGCGCCAGGTCGTCGTCCGGGGTGCGGGGATAGGCGTCGAGATC
TTCCCCGGCACCGAATTGCATCGGGTTGACGAAGATCGACACGACGACGACCGATCCGGGCACCCGCTTG
GCCGCACGCACCAACGCGAGGTGGCCTTCGTGCAGCGCACCCATAGTAGGCACCAACATCACTCGCCGGC
CGGTGAGTCGCAGTGCGCGACTGACATCGGCGACATCCCCCGGTGCCGAGTACACATTGAGTTCACCGGG
ATGGAACGCAGGAATCGTCA

Protein Sequence : Show Sequence

>gi|15610738|ref|NP_218119.1| pantoate--beta-alanine ligase [Mycobacterium tuberculosis H37Rv]
MTIPAFHPGELNVYSAPGDVADVSRALRLTGRRVMLVPTMGALHEGHLALVRAAKRVPGSVVVVSIFVNP
MQFGAGEDLDAYPRTPDDDLAQLRAEGVEIAFTPTTAAMYPDGLRTTVQPGPLAAELEGGPRPTHFAGVL
TVVLKLLQIVRPDRVFFGEKDYQQLVLIRQLVADFNLDVAVVGVPTVREADGLAMSSRNRYLDPAQRAAA
VALSAALTAAAHAATAGAQAALDAARAVLDAAPGVAVDYLELRDIGLGPMPLNGSGRLLVAARLGTTRLL
DNIAIEIGTFAGTDRPDGYRAILESHWRN

Molecule Role : Virmugen
Molecule Role Annotation : A panCD mutant is attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis (Sambandamurthy et al., 2002).
Related Vaccine(s): Mycobacterium tuberculosis panCD mutant vaccine , Mycobacterium tuberculosis RD1/panCD mutant vaccine

24. panD

Gene Name : panD
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 885596
NCBI Protein GI : 15610737
Locus Tag : Rv3601c
Genbank Accession : BX842583
Protein Accession : NP_218118
Taxonomy ID : 83332
Gene Starting Position : 4043861
Gene Ending Position : 4044280
Gene Strand (Orientation) : -
Protein Name : aspartate alpha-decarboxylase
Protein pI : 4.69
Protein Weight : 13595.37
Protein Length : 139
Protein Note : Converts L-aspartate to beta-alanine and provides the major route of beta-alanine production in bacteria. Beta-alanine is essential for the biosynthesis of pantothenate (vitamin B5)

DNA Sequence : Show Sequence

>gi|57116681:4043861-4044280 Mycobacterium tuberculosis H37Rv, complete genome
GCTATCCCACACCGAGCCGGGGGTCTAGCAGCTCGCCCGCGTTTTCGGGCACAAATGCCGGATCGTGGCC
CATGTCGATCGGTTTGTTGTAAGCGTCGACAAACACGATCCGCGGCTGGTATGTGCGGGCCCGGGCGTCG
TCCATCGTCGCGTACGCAATCAGAATCACCAGATCCCCCGGATGCACCAAGTGCGCGGCGGCACCGTTGA
TGCCAATCACACCACTGCCGCGTTCGCCGGTGATCGCGTAGGTGACCAGTCGAGCACCGTTGTCGATATC
GACGATGGTTACCTGTTCGCCTTCCAGCAGGTCGGCGGCGTCCATCAAGTCGGCATCGATGGTCACCGAG
CCGACGTAGTGCAGGTCGGCGCAGGTCACCGTGGCGCGGTGGATCTTCGACTTCAGCATCGTCCGTAACA

Protein Sequence : Show Sequence

>gi|15610737|ref|NP_218118.1| aspartate alpha-decarboxylase [Mycobacterium tuberculosis H37Rv]
MLRTMLKSKIHRATVTCADLHYVGSVTIDADLMDAADLLEGEQVTIVDIDNGARLVTYAITGERGSGVIG
INGAAAHLVHPGDLVILIAYATMDDARARTYQPRIVFVDAYNKPIDMGHDPAFVPENAGELLDPRLGVG

Molecule Role : Virmugen
Molecule Role Annotation : A panCD mutant is attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis (Sambandamurthy et al., 2002).
Related Vaccine(s): Mycobacterium tuberculosis panCD mutant vaccine , Mycobacterium tuberculosis RD1/panCD mutant vaccine

25. PE20

Gene Name : PE20
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0012371
NCBI Gene ID : 885537
NCBI Protein GI : 57116919
Locus Tag : Rv1806
Genbank Accession : JLDD01000021
Protein Accession : YP_177843
Taxonomy ID : 83332
Gene Starting Position : 2048071
Gene Ending Position : 2048370
Gene Strand (Orientation) : +
Protein Name : PE family protein PE20
Protein pI : 4.19
Protein Weight : 8927.3
Protein Length : 99
Protein Note : Rv1806, (MTV049.28), len: 99 aa. Member of the Mycobacterium tuberculosis PE family of gly-, ala-rich proteins (see citation below), most similar to Rv1788|MTV049.10|AL022021 (99 aa), FASTA scores: opt: 334, E(): 4.7 e-15, (59.8% identity in 97 aa overlap). TBparse score is 0.932.

DNA Sequence : Show Sequence

>NC_000962.3:2048071-2048370 Mycobacterium tuberculosis H37Rv, complete genome
CATGGCGTTTGTTCTTGTCTGTCCAGATGCGCTGGCCATCGCGGCCGGTCAGTTGCGCCATGTTGGATCG
GTGATAGCCGCGCGGAATGCGGTCGCGGCACCGGCAACTGCCGAATTGGCCCCGGCGGCCGCTGACGAAG
TATCAGCTTTGACTGCAACACAATTCAACTTCCATGCCGCCATGTACCAAGCGGTCGGCGCCCAGGCGAT
CGCCATGAATGAGGCGTTCGTCGCGATGTTGGGCGCCAGCGCGGATTCTTACGCGGCTACCGAAGCCGCC
AACATCATTGCTGTGAGCTA

Protein Sequence : Show Sequence

>YP_177843.1 PE family protein PE20 [Mycobacterium tuberculosis H37Rv]
MAFVLVCPDALAIAAGQLRHVGSVIAARNAVAAPATAELAPAAADEVSALTATQFNFHAAMYQAVGAQAI
AMNEAFVAMLGASADSYAATEAANIIAVS

Molecule Role : Protective antigen
Molecule Role Annotation : A selection of previously identified protective Mycobacterium tuberculosis DNA vaccines were re-formulated as proteins and administered with a Th1-inducing adjuvant to help stimulate the relevant immune responses necessary for protection. One candidate (Rv1806-1807) induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M. tuberculosis in the lungs (Vipond et al., 2006).
Related Vaccine(s): DNA vaccine Rv1806-1807

26. PepA

Gene Name : PepA
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010953
NCBI Gene ID : 886924
NCBI Protein GI : 15607267
Locus Tag : Rv0125
Genbank Accession : CP003248
Protein Accession : NP_214639
Taxonomy ID : 83332
Gene Starting Position : 151147
Gene Ending Position : 152214
Gene Strand (Orientation) : +
Protein Name : serine protease PepA
Protein pI : 4.91
Protein Weight : 30958.56
Protein Length : 355
Protein Note : Rv0125, (MTCI418B.07, MTB32A), len: 355 aa. Probable pepA (alternate gene name: mtb32a), serine protease (EC 3.4.21.-) (see Skeiky et al., 1999), highly similar to other proteases e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa), FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in 346 aa overlap). Also similar to Q50320 34 kDa PROTEIN PRECURSOR from Mycobacterium tuberculosis (361 aa), FASTA scores: opt: 1689, E(): 0, (70.7% identity in 362 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Has a putative signal sequence at the N-terminus. BELONGS TO THE SERINE PROTEASE FAMILY.; mtb32a

DNA Sequence : Show Sequence

>NC_000962.3:151147-152214 Mycobacterium tuberculosis H37Rv, complete genome
GATGAGCAATTCGCGCCGCCGCTCACTCAGGTGGTCATGGTTGCTGAGCGTGCTGGCTGCCGTCGGGCTG
GGCCTGGCCACGGCGCCGGCCCAGGCGGCCCCGCCGGCCTTGTCGCAGGACCGGTTCGCCGACTTCCCCG
CGCTGCCCCTCGACCCGTCCGCGATGGTCGCCCAAGTGGGGCCACAGGTGGTCAACATCAACACCAAACT
GGGCTACAACAACGCCGTGGGCGCCGGGACCGGCATCGTCATCGATCCCAACGGTGTCGTGCTGACCAAC
AACCACGTGATCGCGGGCGCCACCGACATCAATGCGTTCAGCGTCGGCTCCGGCCAAACCTACGGCGTCG
ATGTGGTCGGGTATGACCGCACCCAGGATGTCGCGGTGCTGCAGCTGCGCGGTGCCGGTGGCCTGCCGTC
GGCGGCGATCGGTGGCGGCGTCGCGGTTGGTGAGCCCGTCGTCGCGATGGGCAACAGCGGTGGGCAGGGC
GGAACGCCCCGTGCGGTGCCTGGCAGGGTGGTCGCGCTCGGCCAAACCGTGCAGGCGTCGGATTCGCTGA
CCGGTGCCGAAGAGACATTGAACGGGTTGATCCAGTTCGATGCCGCGATCCAGCCCGGTGATTCGGGCGG
GCCCGTCGTCAACGGCCTAGGACAGGTGGTCGGTATGAACACGGCCGCGTCCGATAACTTCCAGCTGTCC
CAGGGTGGGCAGGGATTCGCCATTCCGATCGGGCAGGCGATGGCGATCGCGGGCCAGATCCGATCGGGTG
GGGGGTCACCCACCGTTCATATCGGGCCTACCGCCTTCCTCGGCTTGGGTGTTGTCGACAACAACGGCAA
CGGCGCACGAGTCCAACGCGTGGTCGGGAGCGCTCCGGCGGCAAGTCTCGGCATCTCCACCGGCGACGTG
ATCACCGCGGTCGACGGCGCTCCGATCAACTCGGCCACCGCGATGGCGGACGCGCTTAACGGGCATCATC
CCGGTGACGTCATCTCGGTGACCTGGCAAACCAAGTCGGGCGGCACGCGTACAGGGAACGTGACATTGGC
CGAGGGACCCCCGGCCTG

Protein Sequence : Show Sequence

>NP_214639.1 serine protease PepA [Mycobacterium tuberculosis H37Rv]
MSNSRRRSLRWSWLLSVLAAVGLGLATAPAQAAPPALSQDRFADFPALPLDPSAMVAQVGPQVVNINTKL
GYNNAVGAGTGIVIDPNGVVLTNNHVIAGATDINAFSVGSGQTYGVDVVGYDRTQDVAVLQLRGAGGLPS
AAIGGGVAVGEPVVAMGNSGGQGGTPRAVPGRVVALGQTVQASDSLTGAEETLNGLIQFDAAIQPGDSGG
PVVNGLGQVVGMNTAASDNFQLSQGGQGFAIPIGQAMAIAGQIRSGGGSPTVHIGPTAFLGLGVVDNNGN
GARVQRVVGSAPAASLGISTGDVITAVDGAPINSATAMADALNGHHPGDVISVTWQTKSGGTRTGNVTLA
EGPPA

Molecule Role : Protective antigen
Related Vaccine(s): Mtb72f fusion protein

27. PhoP

Gene Name : PhoP
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 888772
NCBI Protein GI : 15607897
Locus Tag : Rv0757
Genbank Accession : BX842574
Protein Accession : NP_215271
3D structure: PDB ID : 3R0J
Taxonomy ID : 83332
Gene Starting Position : 851607
Gene Ending Position : 852350
Gene Strand (Orientation) : ?
Protein Name : two component system response transcriptional positive regulator PHOP
Protein pI : 6.31
Protein Weight : 24835.35
Protein Length : 247
Protein Note : Rv0757, (MTCY369.02), len: 247 aa. Possible phoP, two component system response phosphate regulon transcriptional regulator (see citations below), highly similar to various transcriptional regulators e.g. CAC32360.1|AL583945 putative two component system response regulator from Streptomyces coelicolor (271 aa); T45446 probable two-component response regulator from Mycobacterium leprae (253 aa); and similar to phoP proteins e.g. P13792|PHOP_BACSU alkaline phosphatase synthesis transcription regulatory protein from Bacillus subtilis (240 aa), FASTA scores: opt: 594, E(): 2.3e-33, (41.0% identity in 234 aa overlap); etc. Also highly similar to Rv3765c from Mycobacterium tuberculosis (234 aa), Rv1033c (257 aa), RV0903c|MTCY31.31c|Q10531 (236 aa), FASTA score: (45.4% identity in 229 aa overlap); MTCY10G2_16 and MTU88959_1.

DNA Sequence : Show Sequence

>gi|57116681:851607-852350 Mycobacterium tuberculosis H37Rv, complete genome
AATGCGGAAAGGGGTTGATCTCGTGACGGCGGGAACCCCAGGCGAAAACACCACACCGGAGGCTCGTGTC
CTCGTGGTCGATGATGAGGCCAACATCGTTGAACTGCTGTCGGTGAGCCTCAAGTTCCAGGGCTTTGAAG
TCTACACCGCGACCAACGGGGCACAGGCGCTGGATCGGGCCCGGGAAACCCGGCCGGACGCGGTGATCCT
CGATGTGATGATGCCCGGGATGGACGGCTTTGGGGTGCTGCGCCGGCTGCGCGCCGACGGCATCGATGCC
CCGGCGTTGTTCCTGACGGCCCGTGACTCGCTACAGGACAAGATCGCGGGTCTGACCCTGGGTGGTGACG
ACTATGTGACAAAGCCCTTCAGTTTGGAGGAGGTCGTGGCCAGGCTGCGGGTCATCCTGCGACGCGCGGG
CAAGGGCAACAAGGAACCACGTAATGTTCGACTGACGTTCGCCGATATCGAGCTCGACGAGGAGACCCAC
GAAGTGTGGAAGGCGGGCCAACCGGTGTCGCTGTCGCCCACCGAATTCACCCTGCTGCGCTATTTCGTGA
TCAACGCGGGCACCGTGCTGAGCAAGCCTAAGATTCTCGACCACGTTTGGCGCTACGACTTCGGTGGTGA
TGTCAACGTCGTCGAGTCCTACGTGTCGTATCTGCGCCGCAAGATCGACACTGGGGAGAAGCGGCTGCTG
CACACGCTGCGCGGGGTGGGCTACGTACTGCGGGAGCCTCGATG

Protein Sequence : Show Sequence

>gi|15607897|ref|NP_215271.1| two component system response transcriptional positive regulator PHOP [Mycobacterium tuberculosis H37Rv]
MRKGVDLVTAGTPGENTTPEARVLVVDDEANIVELLSVSLKFQGFEVYTATNGAQALDRARETRPDAVIL
DVMMPGMDGFGVLRRLRADGIDAPALFLTARDSLQDKIAGLTLGGDDYVTKPFSLEEVVARLRVILRRAG
KGNKEPRNVRLTFADIELDEETHEVWKAGQPVSLSPTEFTLLRYFVINAGTVLSKPKILDHVWRYDFGGD
VNVVESYVSYLRRKIDTGEKRLLHTLRGVGYVLREPR

Molecule Role : Virmugen
Molecule Role Annotation : Study evaluated immunogenicity and protection against Mycobacterium tuberculosis in rhesus monkeys with an attenuated M. tuberculosis with a disrupted phoP gene (SO2) as a single-dose vaccine. SO2 treatment showed reduced average lung bacterial counts (1.0 and 1.2 log respectively, compared with 0.4 log for BCG) and significant protective effect by reduction in C-reactive protein levels, body weight loss, and decrease of erythrocyte-associated hematologic parameters (MCV, MCH, Hb, Ht) as markers of inflammatory infection, all relative to non-vaccinated controls (Verreck et al., 2009).
Related Vaccine(s): M. tuberculosis phoP mutant SO2

28. PPE14

Gene Name : PPE14
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0012374
NCBI Gene ID : 885069
NCBI Protein GI : 57116795
Locus Tag : Rv0915c
Genbank Accession : AL123456
Protein Accession : YP_177765
Taxonomy ID : 83332
Gene Starting Position : 1020057
Gene Ending Position : 1021328
Gene Strand (Orientation) : -
Protein Name : PPE family protein PPE14
Protein pI : 4.31
Protein Weight : 39057.75
Protein Length : 423
Protein Note : Rv0915c, (MTCY21C12.09c), len: 423 aa. PPE14 (alternate gene name: MTB41). Member of the Mycobacterium tuberculosis PPE family (see citation below), highly similar to many e.g. Rv1807 from Mycobacterium tuberculosis (403 aa), FASTA scores: opt: 966, E(): 4.4e-30, (45.7% identity in 392 aa overlap); etc. Contains PS00626 Regulator of chromosome condensation (RCC1) signature 2.; MTB41

DNA Sequence : Show Sequence

>NC_000962.3:1020057-1021328 Mycobacterium tuberculosis H37Rv, complete genome
TTTACCGCGGGGGGTTTCCGGGCGGCGGCTGCTCTCTAATCACAACTACCGGGGGTTTGCGGCCGTCCTC
TTGGCCGTCAGTGCTGGTGCCGCTACGGGTGCCGCCACCGCCCGTCGTGCCGCGTGCGGCCAGGCTCGCC
AAAGCCATCCCGCTGAGCAGGCCTGCCGGCATCCCGTTTAGGGCCGTCGGGTCGGCGCCGGCGCTGGAGC
TGAAGGTGGGTGTTGCCTGAACGGCGAGCTGGATCTCCGGGGCGGCCGTGGTCCAGCTGTGCGGCACCGA
CAACGCTCCGACTAATGCTGCGTGGCCGACGCCCGCGGACACCGGCGCCGCGCCCCCGAAGGGGCCCCAG
TGCGGCTCCGGCTCGTCGGTCGCCGAACTCAGTGGATGGCCCTGCGTCGGTCCCAGCCCGCCGGCGTTCC
CGTATAGGCCGATGTGCCAGGGTCTGGCCGTGTTCGTGATCGCGAGCGCAATGCTGCCGGTCGCGATGGA
TGCAATGTAGAGCGCGATCACGTCCAATTCCCCTATCGGGGTGGGGATCACTATCGGCTGAGCGGATCCG
ACTTGCGGGTTGAGGGTCGACGCGATCCCCAACAGTCCCGATGTCAGCGGATCAGCGTTGGCGGCCAATG
CGGACAGAATGTCGCTCAGGATCCCCGGGGGCAGCTGGGCCAGTGTCGCCTGTGCATCCGCAACGGCGCC
CGCACCGGCGGCTTGGGTCGCCGCGGCTGCGGCCGCGGGCCCGGCCGGGCCGGTGCCTTGCACGGGTGGA
GTGAACGGCGGCAACGCCGACGCGGCCGCAGATGCCCCCTCATAGCTGTACATCACGGCAGCGTCTTGGG
CCCACATTTCGGCATACTCGGCCTGGGTAGCCGCGATCGCCGCACTGTTTTGCCCCAGAATGTTCGCCGC
GACCAGCGACATCAACCGGCTGCGGTTGGCCGCGACGAGGGATGGTGGCACCGTCATCGCGAACGCCGTC
CCAAACGCTTCCGCCGCTGCCCTCGCCTGTGTGGCCGTCTCCTTCGCCAGCGCCGCCGTGGCGGCCAGCC
ACCCCACATACGGCGTTGCCGCGGCCGCCATCGCGGCCGCCGCCGGCCCCATCCACGGCTCAACGATCAG
CGTCGACACCACCGATCCATACGAGACCGCGGCGGAAGTCAACTCCGCGGCCACACCGTCCCAGGCGGCC
GCGGCGGCTAGCATCGACTCCGGCCCCGGACCGGAATACATTCGGCTTGAATTCACTTCCGGAGGTAAAA
GCCCGAAATCCA

Protein Sequence : Show Sequence

>YP_177765.1 PPE family protein PPE14 [Mycobacterium tuberculosis H37Rv]
MDFGLLPPEVNSSRMYSGPGPESMLAAAAAWDGVAAELTSAAVSYGSVVSTLIVEPWMGPAAAAMAAAAT
PYVGWLAATAALAKETATQARAAAEAFGTAFAMTVPPSLVAANRSRLMSLVAANILGQNSAAIAATQAEY
AEMWAQDAAVMYSYEGASAAASALPPFTPPVQGTGPAGPAAAAAATQAAGAGAVADAQATLAQLPPGILS
DILSALAANADPLTSGLLGIASTLNPQVGSAQPIVIPTPIGELDVIALYIASIATGSIALAITNTARPWH
IGLYGNAGGLGPTQGHPLSSATDEPEPHWGPFGGAAPVSAGVGHAALVGALSVPHSWTTAAPEIQLAVQA
TPTFSSSAGADPTALNGMPAGLLSGMALASLAARGTTGGGGTRSGTSTDGQEDGRKPPVVVIREQPPPGN
PPR

Molecule Role : Protective antigen
Molecule Role Annotation : immunization with the soluble recombinant rMTB41 protein plus adjuvant, a regimen known to generate primarily CD4+ T cells, resulted in induction of protection comparable to BCG in two well-established animal models of tuberculosis (mice and guinea pigs) (Skeiky et al., 2005).

29. PPE18

Gene Name : PPE18
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010954
NCBI Gene ID : 886073
NCBI Protein GI : 57116841
Locus Tag : Rv1196
Genbank Accession : AL123456
Protein Accession : YP_177795
Taxonomy ID : 83332
Gene Starting Position : 1339348
Gene Ending Position : 1340523
Gene Strand (Orientation) : +
Protein Name : PPE family protein PPE18
Protein pI : 4.81
Protein Weight : 36280.96
Protein Length : 391
Protein Note : Rv1196, (MTCI364.08), len: 391 aa. PPE18 (alternate gene name: mtb39a). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to others e.g. Y07P_MYCTU|Q11031 hypothetical 40.0 kDa protein cy02b10.25c (396 aa), FASTA scores: opt: 2124, E(): 0, (85.1% identity in 397 aa overlap). Note that expression of Rv1196 was demonstrated in lysates by immunodetection (see Dillon et al., 1999).; mtb39a

DNA Sequence : Show Sequence

>NC_000962.3:1339348-1340523 Mycobacterium tuberculosis H37Rv, complete genome
AATGGTGGATTTCGGGGCGTTACCACCGGAGATCAACTCCGCGAGGATGTACGCCGGCCCGGGTTCGGCC
TCGCTGGTGGCCGCGGCTCAGATGTGGGACAGCGTGGCGAGTGACCTGTTTTCGGCCGCGTCGGCGTTTC
AGTCGGTGGTCTGGGGTCTGACGGTGGGGTCGTGGATAGGTTCGTCGGCGGGTCTGATGGTGGCGGCGGC
CTCGCCGTATGTGGCGTGGATGAGCGTCACCGCGGGGCAGGCCGAGCTGACCGCCGCCCAGGTCCGGGTT
GCTGCGGCGGCCTACGAGACGGCGTATGGGCTGACGGTGCCCCCGCCGGTGATCGCCGAGAACCGTGCTG
AACTGATGATTCTGATAGCGACCAACCTCTTGGGGCAAAACACCCCGGCGATCGCGGTCAACGAGGCCGA
ATACGGCGAGATGTGGGCCCAAGACGCCGCCGCGATGTTTGGCTACGCCGCGGCGACGGCGACGGCGACG
GCGACGTTGCTGCCGTTCGAGGAGGCGCCGGAGATGACCAGCGCGGGTGGGCTCCTCGAGCAGGCCGCCG
CGGTCGAGGAGGCCTCCGACACCGCCGCGGCGAACCAGTTGATGAACAATGTGCCCCAGGCGCTGCAACA
GCTGGCCCAGCCCACGCAGGGCACCACGCCTTCTTCCAAGCTGGGTGGCCTGTGGAAGACGGTCTCGCCG
CATCGGTCGCCGATCAGCAACATGGTGTCGATGGCCAACAACCACATGTCGATGACCAACTCGGGTGTGT
CGATGACCAACACCTTGAGCTCGATGTTGAAGGGCTTTGCTCCGGCGGCGGCCGCCCAGGCCGTGCAAAC
CGCGGCGCAAAACGGGGTCCGGGCGATGAGCTCGCTGGGCAGCTCGCTGGGTTCTTCGGGTCTGGGCGGT
GGGGTGGCCGCCAACTTGGGTCGGGCGGCCTCGGTCGGTTCGTTGTCGGTGCCGCAGGCCTGGGCCGCGG
CCAACCAGGCAGTCACCCCGGCGGCGCGGGCGCTGCCGCTGACCAGCCTGACCAGCGCCGCGGAAAGAGG
GCCCGGGCAGATGCTGGGCGGGCTGCCGGTGGGGCAGATGGGCGCCAGGGCCGGTGGTGGGCTCAGTGGT
GTGCTGCGTGTTCCGCCGCGACCCTATGTGATGCCGCATTCTCCGGCGGCCGGCTA

Protein Sequence : Show Sequence

>YP_177795.1 PPE family protein PPE18 [Mycobacterium tuberculosis H37Rv]
MVDFGALPPEINSARMYAGPGSASLVAAAQMWDSVASDLFSAASAFQSVVWGLTVGSWIGSSAGLMVAAA
SPYVAWMSVTAGQAELTAAQVRVAAAAYETAYGLTVPPPVIAENRAELMILIATNLLGQNTPAIAVNEAE
YGEMWAQDAAAMFGYAAATATATATLLPFEEAPEMTSAGGLLEQAAAVEEASDTAAANQLMNNVPQALQQ
LAQPTQGTTPSSKLGGLWKTVSPHRSPISNMVSMANNHMSMTNSGVSMTNTLSSMLKGFAPAAAAQAVQT
AAQNGVRAMSSLGSSLGSSGLGGGVAANLGRAASVGSLSVPQAWAAANQAVTPAARALPLTSLTSAAERG
PGQMLGGLPVGQMGARAGGGLSGVLRVPPRPYVMPHSPAAG

Molecule Role : Protective antigen
Related Vaccine(s): Mtb72f fusion protein

30. PPE31

Gene Name : PPE31
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0012372
NCBI Gene ID : 885072
NCBI Protein GI : WP_003409179
Locus Tag : Rv1807
Genbank Accession : AL123456
Protein Accession : YP_177653
Other Database IDs : CDD:227938
Taxonomy ID : 1763
Gene Starting Position : 2048397
Gene Ending Position : 2049596
Gene Strand (Orientation) : +
Protein Name : PPE family protein
Protein pI : 4.1
Protein Weight : 39253.11
Protein Length : 475
Protein Note : PPE-repeat protein [Function unknown]

DNA Sequence : Show Sequence

>NC_000962.3:2048397-2049596 Mycobacterium tuberculosis H37Rv, complete genome
ACTTGACTTCGCCACGCTACCGCCCGAAATCAACTCGGCGCGTATGTATTCCGGCGCGGGCTCGGCCCCG
ATGCTGGCCGCAGCGTCAGCCTGGCACGGCTTGTCCGCAGAACTGCGCGCCAGCGCACTGTCATACAGCT
CGGTGCTTTCGACGCTGACCGGTGAAGAATGGCACGGTCCGGCGTCGGCATCGATGACAGCCGCGGCCGC
CCCCTACGTGGCCTGGATGAGCGTCACCGCCGTCCGGGCCGAGCAGGCCGGGGCACAGGCGGAGGCTGCC
GCTGCAGCGTACGAAGCCGCGTTCGCAGCAACGGTGCCCCCGCCGGTCATCGAGGCCAACCGCGCCCAGC
TCATGGCGCTGATCGCCACCAATGTGCTAGGCCAAAACGCCCCCGCGATCGCGGCCACCGAGGCCCAGTA
CGCCGAAATGTGGTCCCAGGACGCGATGGCCATGTACGGCTACGCCGGCGCCTCGGCAGCCGCTACCCAG
CTGACCCCGTTCACCGAGCCGGTGCAGACTACCAACGCGTCCGGCCTGGCGGCCCAGTCGGCTGCGATTG
CCCACGCCACCGGCGCCTCGGCTGGTGCTCAGCAAACGACGCTGTCGCAGCTGATCGCCGCCATACCGTC
TGTACTGCAAGGACTTTCGTCATCGACTGCAGCCACGTTCGCGTCGGGGCCGTCCGGATTGCTGGGCATT
GTCGGGTCTGGATCTTCCTGGCTCGACAAACTCTGGGCGTTACTGGACCCCAACTCCAATTTCTGGAACA
CGATAGCTTCGTCCGGACTGTTCTTGCCGAGTAACACGATTGCGCCCTTTTTGGGTCTACTCGGCGGCGT
GGCAGCTGCGGATGCGGCCGGGGATGTGTTGGGAGAGGCCACCAGTGGCGGGCTCGGTGGCGCGCTGGTG
GCGCCGCTTGGCTCAGCGGGCGGGCTAGGCGGCACTGTCGCGGCCGGCCTGGGCAACGCGGCCACCGTCG
GAACCTTGTCGGTGCCGCCGAGCTGGACGGCGGCCGCACCACTAGCCAGCCCCTTGGGCTCCGCGTTGGG
AGGCACACCGATGGTGGCACCGCCCCCAGCAGTGGCGGCCGGCATGCCCGGAATGCCTTTCGGCACCATG
GGCGGTCAAGGCTTCGGGCGTGCCGTGCCCCAGTATGGCTTCCGCCCCAACTTCGTCGCACGACCGCCCG
CCGCCGGGTG

Protein Sequence : Show Sequence

>WP_003409179.1 MULTISPECIES: PPE family protein [Mycobacterium]
MTAALDFATLPPEINSARMYSGAGSAPMLAAASAWHGLSAELRASALSYSSVLSTLTGEEWHGPASASMT
AAAAPYVAWMSVTAVRAEQAGAQAEAAAAAYEAAFAATVPPPVIEANRAQLMALIATNVLGQNAPAIAAT
EAQYAEMWSQDAMAMYGYAGASAAATQLTPFTEPVQTTNASGLAAQSAAIAHATGASAGAQQTTLSQLIA
AIPSVLQGLSSSTAATSASGPSGLLGILGSGSSWLDKLWALLDPNSNFWNTIASSGLFLPSNTIAPFLGL
LGGVAAADAAGDVLGEATSGGLGGALVAPLGSAGGLGGTVAAGLGNAATVGTLSVPPSWTAAAPLASPLG
SALGGTPMVAPPPAVAAGMPGMPFGTMGGQGFGRAVPQYGFRPNFVARPPAAG

Molecule Role : Protective antigen
Molecule Role Annotation : A selection of previously identified protective Mycobacterium tuberculosis DNA vaccines were re-formulated as proteins and administered with a Th1-inducing adjuvant to help stimulate the relevant immune responses necessary for protection. One candidate (Rv1806-1807) induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M. tuberculosis in the lungs (Vipond et al., 2006).
Related Vaccine(s): DNA vaccine Rv1806-1807

31. PPE42

Gene Name : PPE42
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Protein GI : YP_177893
Other Database IDs : CDD:307116
CDD:279771
CDD:311933
Taxonomy ID : 83332
Gene Strand (Orientation) : ?
Protein Name : PPE family protein PPE42
Protein pI : 4.26
Protein Weight : 57135.65
Protein Length : 663
Protein Note : PPE family; pfam00823

Protein Sequence : Show Sequence

>YP_177893.1 PPE family protein PPE42 [Mycobacterium tuberculosis H37Rv]
MNFAVLPPEVNSARIFAGAGLGPMLAAASAWDGLAEELHAAAGSFASVTTGLAGDAWHGPASLAMTRAAS
PYVGWLNTAAGQAAQAAGQARLAASAFEATLAATVSPAMVAANRTRLASLVAANLLGQNAPAIAAAEAEY
EQIWAQDVAAMFGYHSAASAVATQLAPIQEGLQQQLQNVLAQLASGNLGSGNVGVGNIGNDNIGNANIGF
GNRGDANIGIGNIGDRNLGIGNTGNWNIGIGITGNGQIGFGKPANPDVLVVGNGGPGVTALVMGGTDSLL
PLPNIPLLEYAARFITPVHPGYTATFLETPSQFFPFTGLNSLTYDVSVAQGVTNLHTAIMAQLAAGNEVV
VFGTSQSATIATFEMRYLQSLPAHLRPGLDELSFTLTGNPNRPDGGILTRFGFSIPQLGFTLSGATPADA
YPTVDYAFQYDGVNDFPKYPLNVFATANAIAGILFLHSGLIALPPDLASGVVQPVSSPDVLTTYILLPSQ
DLPLLVPLRAIPLLGNPLADLIQPDLRVLVELGYDRTAHQDVPSPFGLFPDVDWAEVAADLQQGAVQGVN
DALSGLGLPPPWQPALPRLF

Molecule Role : Protective antigen
Molecule Role Annotation : ID93/GLA-SE protects against TB and MDR-TB in animals.
Related Vaccine(s): ID93/GLA-SE

32. proC

Gene Name : proC
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 887256
NCBI Protein GI : 15607641
Locus Tag : Rv0500
Genbank Accession : BX842573
Protein Accession : NP_215014
Taxonomy ID : 83332
Gene Starting Position : 590082
Gene Ending Position : 590969
Gene Strand (Orientation) : ?
Protein Name : pyrroline-5-carboxylate reductase
Protein pI : 4.42
Protein Weight : 26600.99
Protein Length : 295
Protein Note : catalyzes the formation of L-proline from pyrroline-5-carboxylate

DNA Sequence : Show Sequence

>gi|57116681:590082-590969 Mycobacterium tuberculosis H37Rv, complete genome
GATGCTTTTCGGCATGGCAAGGATCGCGATTATCGGCGGCGGCAGCATCGGTGAGGCATTGCTGTCGGGT
CTGCTGCGGGCGGGCCGGCAGGTCAAAGACCTGGTAGTGGCCGAGCGGATGCCCGATCGCGCCAACTACC
TGGCGCAGACCTATTCGGTGTTGGTGACGTCGGCGGCCGACGCGGTGGAGAACGCGACGTTCGTCGTCGT
CGCGGTCAAACCAGCCGACGTCGAGCCGGTGATCGCGGATCTGGCGAACGCGACTGCGGCGGCCGAAAAC
GACAGTGCTGAGCAGGTGTTCGTCACCGTGGTAGCGGGCATCACGATCGCGTATTTCGAATCCAAGCTAC
CGGCTGGGACGCCAGTGGTGCGTGCGATGCCGAACGCGGCGGCATTGGTGGGAGCGGGGGTTACAGCGCT
GGCCAAAGGCCGCTTTGTCACCCCGCAACAGCTTGAGGAGGTCTCGGCCTTGTTCGACGCGGTCGGCGGC
GTGCTGACCGTTCCGGAATCGCAGTTGGACGCGGTGACCGCGGTGTCCGGCTCGGGTCCGGCCTATTTCT
TTCTGCTGGTCGAGGCCCTGGTGGATGCCGGAGTCGGGGTGGGCTTGAGCCGTCAGGTGGCCACCGATCT
CGCCGCGCAGACAATGGCTGGCTCAGCGGCGATGCTGCTGGAGCGGATGGAGCAAGACCAGGGTGGCGCC
AATGGCGAGCTGATGGGGCTGCGCGTGGACCTTACCGCATCACGGCTGCGCGCCGCGGTTACCTCGCCGG
GCGGTACGACCGCCGCTGCGCTGCGGGAACTCGAACGCGGCGGGTTTCGGATGGCTGTCGACGCGGCGGT
TCAAGCCGCCAAAAGCCGCTCTGAGCAGCTCAGAATTACACCGGAATG

Protein Sequence : Show Sequence

>gi|15607641|ref|NP_215014.1| pyrroline-5-carboxylate reductase [Mycobacterium tuberculosis H37Rv]
MLFGMARIAIIGGGSIGEALLSGLLRAGRQVKDLVVAERMPDRANYLAQTYSVLVTSAADAVENATFVVV
AVKPADVEPVIADLANATAAAENDSAEQVFVTVVAGITIAYFESKLPAGTPVVRAMPNAAALVGAGVTAL
AKGRFVTPQQLEEVSALFDAVGGVLTVPESQLDAVTAVSGSGPAYFFLLVEALVDAGVGVGLSRQVATDL
AAQTMAGSAAMLLERMEQDQGGANGELMGLRVDLTASRLRAAVTSPGGTTAAALRELERGGFRMAVDAAV
QAAKSRSEQLRITPE

Molecule Role : Virmugen
Molecule Role Annotation : A proC mutant is highly attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis that is equal to the protection induced by the current vaccine BCG (Smith et al., 2001).
Related Vaccine(s): Mycobacterium tuberculosis proC mutant vaccine

33. PstS1

Gene Name : PstS1
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0012407
NCBI Gene ID : 885724
NCBI Protein GI : 57116801
Locus Tag : Rv0934
Genbank Accession : AL123456
Protein Accession : YP_177770
3D structure: PDB ID : 1PC3
Taxonomy ID : 83332
Gene Starting Position : 1042114
Gene Ending Position : 1043238
Gene Strand (Orientation) : +
Protein Name : phosphate ABC transporter substrate-binding lipoprotein PstS
Protein pI : 5.02
Protein Weight : 36356.95
Protein Length : 374
Protein Note : Rv0934, (MTCY08D9.05c), len: 374 aa. pstS1 (previously known as phoS1 or phoS), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to Rv0932c|MTCY08D9.07|pstS2 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN from Mycobacterium tuberculosis (370 aa), FASTA scores: opt: 460, E(): 5.9e-19, (31.2% identity in 375 aa overlap); and Rv0928|MTCY21C12.22|pstS3 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 435, E():1.1e-17, (30.0% identity in 380 aa overlap) (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0928c). Also highly similar to MTCY08D9.05c|P15712|PAB_MYCTU PROTEIN ANTIGEN B PRECURSOR from Mycobacterium tuberculosis (374 aa), FASTA scores: opt: 2459, E(): 0, (100% identity in 374 aa overlap). Contains a prokaryotic membrane lipoprotein lipid attachment site (PS00013) at the N-terminus so the 23 aa leader peptide sequence is probably removed. BELONGS TO FAMILY OF PHOSPHATE RECEPTORS FOR BACTERIAL ABC-TYPE LIPOPROTEIN TRANSPORTERS.; phoS1; phoS

DNA Sequence : Show Sequence

>NC_000962.3:1042114-1043238 Mycobacterium tuberculosis H37Rv, complete genome
CGTGAAAATTCGTTTGCATACGCTGTTGGCCGTGTTGACCGCTGCGCCGCTGCTGCTAGCAGCGGCGGGC
TGTGGCTCGAAACCACCGAGCGGTTCGCCTGAAACGGGCGCCGGCGCCGGTACTGTCGCGACTACCCCCG
CGTCGTCGCCGGTGACGTTGGCGGAGACCGGTAGCACGCTGCTCTACCCGCTGTTCAACCTGTGGGGTCC
GGCCTTTCACGAGAGGTATCCGAACGTCACGATCACCGCTCAGGGCACCGGTTCTGGTGCCGGGATCGCG
CAGGCCGCCGCCGGGACGGTCAACATTGGGGCCTCCGACGCCTATCTGTCGGAAGGTGATATGGCCGCGC
ACAAGGGGCTGATGAACATCGCGCTAGCCATCTCCGCTCAGCAGGTCAACTACAACCTGCCCGGAGTGAG
CGAGCACCTCAAGCTGAACGGAAAAGTCCTGGCGGCCATGTACCAGGGCACCATCAAAACCTGGGACGAC
CCGCAGATCGCTGCGCTCAACCCCGGCGTGAACCTGCCCGGCACCGCGGTAGTTCCGCTGCACCGCTCCG
ACGGGTCCGGTGACACCTTCTTGTTCACCCAGTACCTGTCCAAGCAAGATCCCGAGGGCTGGGGCAAGTC
GCCCGGCTTCGGCACCACCGTCGACTTCCCGGCGGTGCCGGGTGCGCTGGGTGAGAACGGCAACGGCGGC
ATGGTGACCGGTTGCGCCGAGACACCGGGCTGCGTGGCCTATATCGGCATCAGCTTCCTCGACCAGGCCA
GTCAACGGGGACTCGGCGAGGCCCAACTAGGCAATAGCTCTGGCAATTTCTTGTTGCCCGACGCGCAAAG
CATTCAGGCCGCGGCGGCTGGCTTCGCATCGAAAACCCCGGCGAACCAGGCGATTTCGATGATCGACGGG
CCCGCCCCGGACGGCTACCCGATCATCAACTACGAGTACGCCATCGTCAACAACCGGCAAAAGGACGCCG
CCACCGCGCAGACCTTGCAGGCATTTCTGCACTGGGCGATCACCGACGGCAACAAGGCCTCGTTCCTCGA
CCAGGTTCATTTCCAGCCGCTGCCGCCCGCGGTGGTGAAGTTGTCTGACGCGTTGATCGCGACGATTTCC
AGCTA

Protein Sequence : Show Sequence

>YP_177770.1 phosphate ABC transporter substrate-binding lipoprotein PstS [Mycobacterium tuberculosis H37Rv]
MKIRLHTLLAVLTAAPLLLAAAGCGSKPPSGSPETGAGAGTVATTPASSPVTLAETGSTLLYPLFNLWGP
AFHERYPNVTITAQGTGSGAGIAQAAAGTVNIGASDAYLSEGDMAAHKGLMNIALAISAQQVNYNLPGVS
EHLKLNGKVLAAMYQGTIKTWDDPQIAALNPGVNLPGTAVVPLHRSDGSGDTFLFTQYLSKQDPEGWGKS
PGFGTTVDFPAVPGALGENGNGGMVTGCAETPGCVAYIGISFLDQASQRGLGEAQLGNSSGNFLLPDAQS
IQAAAAGFASKTPANQAISMIDGPAPDGYPIINYEYAIVNNRQKDAATAQTLQAFLHWAITDGNKASFLD
QVHFQPLPPAVVKLSDALIATISS

Molecule Role : Protective antigen
Molecule Role Annotation : Vaccination with the recombinant PstS-1 antigen and cholera toxin significantly protected against the challenge given 10 days later, but only marginally after 12 weeks (Falero-Diaz et al., 2000).

34. PstS3

Gene Name : PstS3
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0012370
NCBI Gene ID : 885366
NCBI Protein GI : 57116798
Locus Tag : Rv0928
Genbank Accession : AL123456
Protein Accession : YP_177768
Taxonomy ID : 83332
Gene Starting Position : 1034902
Gene Ending Position : 1036014
Gene Strand (Orientation) : +
Protein Name : phosphate ABC transporter substrate-binding lipoprotein PstS
Protein pI : 5.85
Protein Weight : 35472.01
Protein Length : 370
Protein Note : Rv0928, (MTCY21C12.22), len: 370 aa. pstS3 (previously known as phoS2), phosphate-binding lipoprotein component of inorganic phosphate transport system (see citations below), highly similar to others from Mycobacterium leprae e.g. Q50099|PSTS3|PHOS1 phosphate-binding protein 3 precursor (328 aa), FASTA scores: opt: 1772, E(): 0, (79.6% identity in 328 aa overlap); and highly similar to others e.g. AAF74819.1|AF137360_1|AF137360 periplasmic phosphate permease from Mycobacterium avium (369 aa). Also highly similar to Rv0932c|MTCY08D9.07|pstS2 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN (370 aa); and Rv0934|pstS1 PHOSPHATE-BINDING PERIPLASMIC LIPOPROTEIN (374 aa) from Mycobacterium tuberculosis (Mycobacterium tuberculosis seems to have three PstS-like proteins, others being Rv0932c and Rv0934c). Contains lipoprotein signature (PS00013) at N-terminus. BELONGS TO FAMILY OF PHOSPHATE RECEPTORS FOR BACTERIAL ABC-TYPE LIPOPROTEIN TRANSPORTERS.; phoS2

DNA Sequence : Show Sequence

>NC_000962.3:1034902-1036014 Mycobacterium tuberculosis H37Rv, complete genome
ATTGAAACTCAACCGATTTGGTGCCGCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGT
GGTAACGACGACAATGTGACCGGGGGAGGTGCAACCACTGGCCAGGCGTCGGCGAAGGTCGATTGCGGGG
GGAAGAAGACACTCAAAGCCAGTGGGTCGACGGCGCAGGCCAACGCGATGACCCGCTTTGTCAACGTGTT
CGAGCAGGCCTGCCCCGGCCAAACCCTGAACTACACGGCCAATGGTTCGGGCGCTGGAATCAGCGAATTT
AATGGCAACCAAACCGATTTCGGTGGCTCAGATGTACCCCTGAGCAAGGACGAGGCCGCAGCGGCGCAGC
GGCGTTGCGGCTCGCCGGCGTGGAATCTGCCGGTGGTGTTCGGCCCGATCGCGGTTACCTACAACCTCAA
CAGCGTTTCCTCGCTAAATTTGGACGGCCCCACGTTGGCGAAGATCTTCAACGGCTCCATTACGCAGTGG
AACAATCCCGCGATCCAGGCGCTGAACCGCGACTTCACGCTGCCAGGTGAGCGGATTCACGTGGTGTTCC
GCAGCGATGAGTCGGGGACCACGGACAACTTCCAGAGGTACCTGCAGGCCGCGTCCAACGGTGCGTGGGG
TAAGGGCGCTGGAAAGTCGTTCCAAGGCGGCGTCGGTGAGGGCGCGCGGGGTAACGATGGCACGTCAGCG
GCCGCGAAGAACACCCCGGGGTCGATCACCTACAACGAGTGGTCGTTCGCCCAGGCGCAGCACCTGACCA
TGGCCAACATCGTCACTTCGGCTGGTGGGGACCCGGTGGCGATTACTATCGACTCGGTCGGCCAGACGAT
CGCCGGGGCCACCATCTCCGGGGTGGGCAACGACCTGGTGCTCGACACGGACTCGTTCTACCGGCCGAAG
CGTCCCGGCTCCTATCCGATCGTGTTAGCGACATACGAAATCGTTTGCTCGAAGTATCCCGACTCGCAGG
TTGGCACGGCTGTGAAGGCGTTCCTGCAGAGCACTATCGGCGCCGGTCAAAGCGGCCTGGGGGACAACGG
ATACATCCCAATTCCGGACGAGTTCAAATCGAGGCTGTCGACTGCGGTCAACGCGATCGCCTG

Protein Sequence : Show Sequence

>YP_177768.1 phosphate ABC transporter substrate-binding lipoprotein PstS [Mycobacterium tuberculosis H37Rv]
MKLNRFGAAVGVLAAGALVLSACGNDDNVTGGGATTGQASAKVDCGGKKTLKASGSTAQANAMTRFVNVF
EQACPGQTLNYTANGSGAGISEFNGNQTDFGGSDVPLSKDEAAAAQRRCGSPAWNLPVVFGPIAVTYNLN
SVSSLNLDGPTLAKIFNGSITQWNNPAIQALNRDFTLPGERIHVVFRSDESGTTDNFQRYLQAASNGAWG
KGAGKSFQGGVGEGARGNDGTSAAAKNTPGSITYNEWSFAQAQHLTMANIVTSAGGDPVAITIDSVGQTI
AGATISGVGNDLVLDTDSFYRPKRPGSYPIVLATYEIVCSKYPDSQVGTAVKAFLQSTIGAGQSGLGDNG
YIPIPDEFKSRLSTAVNAIA

Molecule Role : Protective antigen
Molecule Role Annotation : Mice vaccinated with PstS-3 DNA demonstrated significant and sustained reduction in bacterial CFU numbers in spleen and lungs for 3 mo after M. tuberculosis challenge, as compared with CFU counts in mice vaccinated with control DNA (Tanghe et al., 1999).

35. RD1

Gene Name : RD1
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 885777
NCBI Protein GI : 15610750
Locus Tag : Rv3614c
Genbank Accession : BX842583
Protein Accession : NP_218131
Taxonomy ID : 83332
Gene Starting Position : 4054141
Gene Ending Position : 4054695
Gene Strand (Orientation) : -
Protein Name : hypothetical protein
Protein pI : 3.73
Protein Weight : 18643.7
Protein Length : 184
Protein Note : Rv3614c, (MTCY07H7B.08), len: 184 aa. Conserved hypothetical protein, equivalent to Q49730|ML0407|B1620_C3_264|MLCL383.03 HYPOTHETICAL 24.2 KDA PROTEIN from Mycobacterium leprae (216 aa) FASTA scores: opt: 899, E(): 1.7e-51, (71.3% identity in 188 aa overlap); and similar to two hypothetical proteins from Mycobacterium leprae: Q9CDD6|ML0056 (169 aa), FASTA scores: opt: 285, E(): 1.2e-11, (38.35% identity in 172 aa overlap); and O33090|MLCB628.19c (338 aa), FASTA scores: opt: 289, E(): 1.2e-11, (38.95% identity in 172 aa overlap). Also highly similar to O69732|Rv3867|MTV027.02 HYPOTHETICAL 19.9 KDA PROTEIN from Mycobacterium tuberculosis (183 aa), FASTA scores: opt: 563, E(): 1e-29, (54.9% identity in 173 aa overlap).

DNA Sequence : Show Sequence

>gi|57116681:4054141-4054695 Mycobacterium tuberculosis H37Rv, complete genome
CTCACCATGGATCGCTCTCGTCGTCTGGTGCTGGACAATCGTCGCTGTAGCGCGTCGCGAACACCTCAGC
TTCTGCTGCCGCGGCTTCTTCCGGCGATGGTAACCCCCAGGTTTCGCCCACGGTCTTACGTAGCAGTGCG
ACGCGGTGTTCATCTGCATCGACCTGTTGACTCATCCTGTCAAGGATGAAGGCGTACTGGGCCGACTGCG
CCTTCTGCCGCGCCAGGTCGGCAATCACCAGGATCTCAGAAGCGAGCTGCGACTCACTCATCCAGGCCAC
CCTGGCCGACAGCTCGACATGGTCAATCCGGCCGTCCATCAGCGTCGATACCGACACCGTGCGTGGGGGA
TTCGTCACGGTAAAAAGCGCGATCTCTTGTTCGGTGTCCGTCTCCGCCTGACCGTGGGCATTGTCCAGGT
CGGGTCCGGTGTCCGGGGTCGCCGCCGACCCGACGCCAATAATCGGATCCGCAGTCCAGCCCTCCGCGCC
GTCGGCACCCCAGAGATCCACGGCGTCGAAATCGTTGCTGTCAAAGTCATTTCCGGGCAAGTCCA

Protein Sequence : Show Sequence

>gi|15610750|ref|NP_218131.1| hypothetical protein Rv3614c [Mycobacterium tuberculosis H37Rv]
MDLPGNDFDSNDFDAVDLWGADGAEGWTADPIIGVGSAATPDTGPDLDNAHGQAETDTEQEIALFTVTNP
PRTVSVSTLMDGRIDHVELSARVAWMSESQLASEILVIADLARQKAQSAQYAFILDRMSQQVDADEHRVA
LLRKTVGETWGLPSPEEAAAAEAEVFATRYSDDCPAPDDESDPW

Molecule Role : Virmugen
Molecule Role Annotation : RD1 and panCD mutants in Mycobacterium tuberculosis were attenuated and provided protection in CD4-deficient mice against an aerosol challenge with virulent M. tuberculosis (Sambandamurthy et al., 2006).
Related Vaccine(s): Mycobacterium tuberculosis RD1/panCD mutant vaccine

36. Rv1813c

Gene Name : Rv1813c
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Protein GI : NP_216329
Other Database IDs : CDD:316353
Taxonomy ID : 83332
Gene Strand (Orientation) : ?
Protein Name : hypothetical protein
Protein pI : 10.16
Protein Weight : 14879.86
Protein Length : 224
Protein Note : Domain of unknown function (DUF4189); pfam13827

Protein Sequence : Show Sequence

>NP_216329.1 hypothetical protein Rv1813c [Mycobacterium tuberculosis H37Rv]
MITNLRRRTAMAAAGLGAALGLGILLVPTVDAHLANGSMSEVMMSEIAGLPIPPIIHYGAIAYAPSGASG
KAWHQRTPARAEQVALEKCGDKTCKVVSRFTRCGAVAYNGSKYQGGTGLTRRAAEDDAVNRLEGGRIVNW
ACN

Molecule Role : Protective antigen
Molecule Role Annotation : ID93/GLA-SE protects against TB and MDR-TB in animals.(Bertholet et al., 2010)
Related Vaccine(s): ID93/GLA-SE

37. Rv2660c

Gene Name : Rv2660c
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 887222
NCBI Protein GI : 15609797
Locus Tag : Rv2660c
Genbank Accession : CP009480
Protein Accession : NP_217176
Taxonomy ID : 83332
Gene Starting Position : 2980962
Gene Ending Position : 2981189
Gene Strand (Orientation) : -
Protein Name : hypothetical protein
Protein pI : 4.63
Protein Weight : 6788.85
Protein Length : 75
Protein Note : Rv2660c, (MTCY441.29c), len: 75 aa (questionable orf). Hypothetical unknown protein.

DNA Sequence : Show Sequence

>NC_000962.3:2980962-2981189 Mycobacterium tuberculosis H37Rv, complete genome
ACTAGTGAAACTGGTTCAATCCCAGTATCGCGCACCACGATTGACCTGCGGTTTCATCCACAAAATCTGG
GCTGCGTGAACTAAATGTGAACTGACTCGGTGCAACCACCGAAAGGTTCCTCTGTTCCGTGCCCACGCCG
ACACCGACGGTGACCCCACCAGATGCGCCTGCCGCCCGCTGGCTAGCCTGGCCTGTTGCTGCAAGCGCCT
GGTCGACGCCCGCTATCA

Protein Sequence : Show Sequence

>NP_217176.1 hypothetical protein Rv2660c [Mycobacterium tuberculosis H37Rv]
MIAGVDQALAATGQASQRAAGASGGVTVGVGVGTEQRNLSVVAPSQFTFSSRSPDFVDETAGQSWCAILG
LNQFH

Molecule Role : Protective antigen
Molecule Role Annotation : When combined with AG85B and ESAT-6, Rv2660c provided mice protection against challenge (Aagaard et al., 2011).

38. SecA2

Gene Name : SecA2
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
VO ID : VO_0010950
NCBI Gene ID : 885594
NCBI Protein GI : 15608958
Locus Tag : Rv1821
Genbank Accession : BX842577
Protein Accession : NP_216337
3D structure: PDB ID : 1NL3
Other Database IDs : EMBL: AE000516
EMBL: BX842577
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
HSSP: O05885
InterPro: IPR000185
InterPro: IPR001650
InterPro: IPR011115
InterPro: IPR011116
InterPro: IPR011130
InterPro: IPR014018
InterPro: IPR014021
KEGG: mtc:MT1869
KEGG: mtu:Rv1821
Pfam: PF01043
Pfam: PF07516
Pfam: PF07517
PIR: F70720
PRINTS: PR00906
PROSITE: PS01312
PROSITE: PS51196
TIGR: MT1869
TubercuList: Rv1821
UniProt: P66785
Taxonomy ID : 83332
Gene Starting Position : 2066456
Gene Ending Position : 2068882
Gene Strand (Orientation) : +
Protein Name : preprotein translocase subunit SecA
Protein pI : 5.11
Protein Weight : 82502.57
Protein Length : 808
Protein Note : SecA2; functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond to SecA2; which is non-essential and seems to play a role in secretion of a subset of proteins
Protein Annotation : FUNCTION: Involved in protein export. Interacts with the secY/secE subunits. SecA has a central role in coupling the hydrolysis of ATP to the transfer of pre-secretory proteins across the membrane (By similarity) (UniProt: P66785)

SUBUNIT: Part of the prokaryotic protein translocation apparatus which comprise secA, secB, secD, secE, secF, secG and secY (By similarity) (UniProt: P66785)

SUBCELLULAR LOCATION: Cell membrane; peripheral membrane protein; cytoplasmic side (By similarity) (UniProt: P66785)

SIMILARITY: Belongs to the secA family (UniProt: P66785)

DNA Sequence : Show Sequence

>gi|57116681:2066456-2068882 Mycobacterium tuberculosis H37Rv, complete genome
CGTGAACGTGCACGGTTGTCCACGAATTGCGGCCTGTCGGTGTACAGACACGCACCCTCGCGGCCGGCCG
GCATTCGCGTACCGTTGGTTTGTGCCCAAGACCACCCGCGCTCAACCCGGCCGGCTGAGCAGCCGATTCT
GGCGATTGCTCGGCGCCAGCACCGAAAAGAACCGGAGCCGCTCCCTGGCGGATGTAACCGCTTCGGCAGA
ATACGACAAGGAAGCTGCCGATCTGTCCGACGAGAAGCTGCGTAAGGCGGCAGGCCTGCTCAACCTCGAC
GACCTCGCGGAGTCCGCCGATATCCCGCAGTTTCTCGCGATTGCCCGGGAAGCCGCCGAGCGGAGGACCG
GGCTGCGACCATTTGATGTGCAGTTGCTTGGCGCGTTGCGCATGCTCGCCGGAGACGTGATCGAGATGGC
CACCGGTGAGGGCAAAACCCTTGCCGGGGCGATCGCGGCCGCCGGTTATGCGCTGGCCGGCCGGCACGTG
CACGTCGTGACGATTAACGATTACCTGGCCCGCCGCGATGCGGAGTGGATGGGCCCGCTGCTGGACGCGA
TGGGCCTGACGGTCGGCTGGATCACCGCGGACTCGACCCCTGACGAGCGCCGGACCGCATATGACCGTGA
TGTCACCTATGCCTCGGTCAACGAGATTGGCTTCGATGTACTGCGCGATCAGTTGGTGACTGATGTCAAT
GACCTGGTATCGCCCAATCCAGACGTGGCTCTCATCGACGAAGCCGACTCCGTGCTGGTCGACGAGGCGC
TGGTGCCCCTGGTGCTGGCCGGAACCACACATCGTGAGACGCCGCGGCTGGAGATCATCCGGCTGGTCGC
TGAGCTTGTTGGCGACAAGGACGCCGACGAGTACTTTGCCACCGATTCCGATAACCGCAATGTCCACTTG
ACCGAGCACGGGGCACGCAAAGTCGAGAAAGCGCTCGGTGGCATCGACCTGTACTCCGAGGAGCACGTCG
GCACCACACTGACTGAGGTCAATGTCGCGCTGCACGCGCATGTGCTCCTGCAACGCGACGTGCACTACAT
CGTCCGCGACGACGCGGTGCACCTGATCAACGCGTCGCGTGGCCGTATCGCGCAACTGCAGCGCTGGCCG
GACGGGTTGCAAGCTGCGGTCGAGGCCAAGGAAGGTATCGAGACCACGGAAACTGGGGAAGTGCTCGACA
CCATCACGGTGCAGGCCCTGATCAACCGGTATGCGACTGTGTGCGGAATGACGGGAACCGCGCTGGCCGC
CGGTGAGCAGCTACGGCAGTTCTACCAGCTCGGTGTCTCACCGATACCACCGAACAAGCCAAACATCCGC
GAGGACGAGGCCGACCGGGTCTACATCACCACTGCAGCCAAGAACGACGGGATCGTCGAGCACATCACCG
AGGTGCACCAGAGGGGGCAGCCTGTGCTGGTCGGTACCCGCGACGTGGCCGAATCCGAGGAACTGCACGA
ACGCCTGGTGCGCCGCGGTGTGCCCGCCGTGGTGCTCAACGCGAAGAACGACGCCGAGGAGGCCCGGGTC
ATCGCCGAGGCCGGCAAATACGGCGCGGTCACGGTGTCAACTCAAATGGCCGGGCGCGGCACCGACATCA
GGCTCGGCGGGTCCGACGAAGCTGACCACGACAGGGTCGCGGAATTGGGCGGCCTGCACGTGGTCGGCAC
TGGCCGTCACCACACCGAGCGGCTAGACAACCAGCTGCGCGGTCGGGCCGGGCGCCAGGGAGATCCCGGG
TCGTCGGTGTTTTTCTCAAGCTGGGAAGACGATGTCGTTGCGGCCAACCTCGACCACAACAAGCTGCCGA
TGGCAACCGACGAAAATGGCCGGATTGTCAGCCCGAGGACGGGTAGTCTGCTCGACCATGCCCAGCGCGT
TGCCGAGGGCCGGTTATTGGATGTGCACGCCAACACGTGGCGCTACAACCAGCTGATCGCCCAGCAGCGC
GCCATCATCGTCGAACGGCGTAACACGTTGTTGCGCACCGTAACCGCGCGTGAGGAACTCGCCGAACTGG
CGCCTAAGCGGTACGAGGAGCTGTCCGACAAAGTATCCGAGGAACGCCTCGAGACGATTTGTCGGCAGAT
CATGCTGTATCACCTCGACCGTGGCTGGGCCGATCACCTGGCGTATCTGGCCGACATCCGGGAGAGCATC
CATCTACGCGCGCTGGGCCGGCAGAACCCACTCGACGAGTTTCACCGGATGGCTGTGGACGCGTTCGCGT
CGCTGGCCGCCGACGCCATCGAGGCGGCTCAACAGACGTTCGAAACCGCGAACGTCCTTGACCACGAGCC
GGGGCTGGACCTGTCCAAACTGGCCCGGCCGACGTCGACATGGACCTACATGGTCAATGACAACCCACTG
TCCGATGACACGCTTTCTGCCCTCAGTCTGCCCGGGGTGTTCCGCTG

Protein Sequence : Show Sequence

>gi|15608958|ref|NP_216337.1| preprotein translocase subunit SecA [Mycobacterium tuberculosis H37Rv]
MNVHGCPRIAACRCTDTHPRGRPAFAYRWFVPKTTRAQPGRLSSRFWRLLGASTEKNRSRSLADVTASAE
YDKEAADLSDEKLRKAAGLLNLDDLAESADIPQFLAIAREAAERRTGLRPFDVQLLGALRMLAGDVIEMA
TGEGKTLAGAIAAAGYALAGRHVHVVTINDYLARRDAEWMGPLLDAMGLTVGWITADSTPDERRTAYDRD
VTYASVNEIGFDVLRDQLVTDVNDLVSPNPDVALIDEADSVLVDEALVPLVLAGTTHRETPRLEIIRLVA
ELVGDKDADEYFATDSDNRNVHLTEHGARKVEKALGGIDLYSEEHVGTTLTEVNVALHAHVLLQRDVHYI
VRDDAVHLINASRGRIAQLQRWPDGLQAAVEAKEGIETTETGEVLDTITVQALINRYATVCGMTGTALAA
GEQLRQFYQLGVSPIPPNKPNIREDEADRVYITTAAKNDGIVEHITEVHQRGQPVLVGTRDVAESEELHE
RLVRRGVPAVVLNAKNDAEEARVIAEAGKYGAVTVSTQMAGRGTDIRLGGSDEADHDRVAELGGLHVVGT
GRHHTERLDNQLRGRAGRQGDPGSSVFFSSWEDDVVAANLDHNKLPMATDENGRIVSPRTGSLLDHAQRV
AEGRLLDVHANTWRYNQLIAQQRAIIVERRNTLLRTVTAREELAELAPKRYEELSDKVSEERLETICRQI
MLYHLDRGWADHLAYLADIRESIHLRALGRQNPLDEFHRMAVDAFASLAADAIEAAQQTFETANVLDHEP
GLDLSKLARPTSTWTYMVNDNPLSDDTLSALSLPGVFR

Molecule Role : Virmugen
Molecule Role Annotation : Vaccination of mice and guinea pigs with a secA2 mutant significantly increased resistance to M. tuberculosis challenge compared with standard M. bovis bacille Calmette-Guérin vaccination (Hinchey et al., 2007). In another study, lysine auxotrophy combined with deletion of the SecA2 gene made a live attenuated vaccine for Mycobacterium tuberculosis. SCID mice was highly protected from challenge after the vaccination (Hinchey et al., 2011).
Related Vaccine(s): M. tuberculosis secA2 mutant , Mycobacterium tuberculosis lysA/secA2 mutant vaccine

39. sigE

Gene Name : sigE
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 888751
NCBI Protein GI : 15608361
Locus Tag : Rv1221
Genbank Accession : BX842575
Protein Accession : NP_215737
Taxonomy ID : 83332
Gene Starting Position : 1364412
Gene Ending Position : 1365185
Gene Strand (Orientation) : ?
Protein Name : RNA polymerase sigma factor SigE
Protein pI : 4.82
Protein Weight : 27091.02
Protein Length : 257
Protein Note : Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; in M. tuberculosis this protein is involved in heat shock, oxidative stress and virulence

DNA Sequence : Show Sequence

>gi|57116681:1364412-1365185 Mycobacterium tuberculosis H37Rv, complete genome
CATGGAACTCCTCGGCGGACCCCGGGTTGGGAATACGGAATCGCAACTTTGCGTTGCCGACGGTGACGAC
TTGCCAACTTATTGCAGTGCAAATTCGGAGGATCTCAATATCACGACCATCACGACCTTGAGTCCGACCA
GCATGTCTCATCCCCAACAGGTCCGCGATGACCAGTGGGTGGAGCCGTCTGACCAATTGCAGGGCACCGC
CGTATTCGACGCCACCGGGGACAAGGCCACCATGCCGTCCTGGGATGAGCTGGTCCGTCAGCACGCCGAT
CGGGTGTACCGGCTGGCTTATCGGCTCTCCGGCAACCAGCACGATGCCGAAGACCTGACCCAGGAGACCT
TTATCAGGGTGTTCCGGTCGGTCCAGAATTACCAGCCGGGCACCTTCGAAGGCTGGCTACACCGCATCAC
CACCAACTTGTTCCTGGACATGGTCCGCCGCCGGGCTCGCATCCGGATGGAGGCGTTACCCGAGGACTAC
GACCGGGTGCCCGCCGATGAGCCCAACCCCGAGCAGATCTACCACGACGCACGGCTGGGACCTGACCTGC
AGGCTGCCTTGGCCTCGCTGCCGCCGGAGTTTCGTGCCGCGGTGGTGCTGTGTGACATCGAGGGTCTGTC
GTACGAGGAGATCGGCGCCACACTGGGCGTGAAGCTCGGGACGGTACGTAGCCGGATACACCGCGGACGC
CAGGCACTGCGGGACTACCTGGCAGCGCACCCCGAACATGGCGAGTGCGCAGTTCACGTCAACCCAGTTC
GCTG

Protein Sequence : Show Sequence

>gi|15608361|ref|NP_215737.1| RNA polymerase sigma factor SigE [Mycobacterium tuberculosis H37Rv]
MELLGGPRVGNTESQLCVADGDDLPTYCSANSEDLNITTITTLSPTSMSHPQQVRDDQWVEPSDQLQGTA
VFDATGDKATMPSWDELVRQHADRVYRLAYRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRIT
TNLFLDMVRRRARIRMEALPEDYDRVPADEPNPEQIYHDARLGPDLQAALASLPPEFRAAVVLCDIEGLS
YEEIGATLGVKLGTVRSRIHRGRQALRDYLAAHPEHGECAVHVNPVR

Molecule Role : Virmugen
Molecule Role Annotation : A sigE mutant is highly attenuated in mice and induced significant protection from challenge with wild type M. tuberculosis, more so than the existing vaccine BCG (Hernandez et al., 2010).
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

40. sigF

Gene Name : sigF
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis CDC1551
NCBI Gene ID : 922492
NCBI Protein GI : 15842877
Locus Tag : MT3385
Genbank Accession : AE000516
Protein Accession : NP_337914
Taxonomy ID : 83331
Gene Starting Position : 3663004
Gene Ending Position : 3663789
Gene Strand (Orientation) : -
Protein Name : RNA polymerase sigma factor SigF
Protein pI : 6.53
Protein Weight : 26809.27
Protein Length : 261
Protein Note : Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this sigma factor is a general stress response regulator; expressed in stationary phase and under nitrogen depletion and cold shock

DNA Sequence : Show Sequence

>gi|50953765:3663004-3663789 Mycobacterium tuberculosis CDC1551 chromosome, complete genome
GCTACTCCAACTGATCCCGTAGCCGTGCCAATGACTTGGCCAGCAGCCGCGACACGTGCATCTGTGAGAT
ACCGACGCGCTCGGCGATCTGCGTTTGGGTCATCGAGTCGAAGAACCTGAGCACCAAGACCGTTCGTTCC
CGCTCGGGCAACGCCTCGAGCAACGGACGAAGCACCTCCCGATTCTCGATCTGGTCAAGACCCGCATCCA
CGTCGCCCAGGGTGTCTGTGATTGCGCGGGCATCGTCGTCGCTGCCGCCACCGCTGTCGATGGACAAGGT
GTGGTAGGAACTACCCGCCAGCAAACCTTCGATAACCTCAGCGCGGTCCATCCCGAGCTCCGCGGCGAGC
TCCGATGCCGACGGCGCCCGCCCGAGCCGCTGCGACAAATCGGCGGTGGCGGTACCTAGCCGCAGATGCA
GTTCCTTGAGACGCCGGGGAACCTTGACCGACCAGCTGTTGTCGCGGAAGTGTCGTCGGACCTCGCCCAT
GATGGTAGGAACCGCGAAGGAGACGAAGTCCGACCCGGTCTTCACGTCGAAGCGAACCGCGGCGTTGACC
AGCCCGACCCGCGCGACCTGAATAAGGTCGTCACGCGGTTCGCCGCGACCCTCGAACCGCCGCGCGATGT
GATCGGCCAGCGGCAAGCACCGCTGAACGATCTTGTCCCGGTGCCGCTGGAATTCCGGTGAGCCGGCAGG
CAAACCAACCAGCTCGCGAAACATCTCCGGAACGTCGGCGTATTCGTTAGCTCGCGATGCAGAACCGCCG
GCAGCGCGCGCCGTCA

Protein Sequence : Show Sequence

>gi|15842877|ref|NP_337914.1| RNA polymerase sigma factor SigF [Mycobacterium tuberculosis CDC1551]
MTARAAGGSASRANEYADVPEMFRELVGLPAGSPEFQRHRDKIVQRCLPLADHIARRFEGRGEPRDDLIQ
VARVGLVNAAVRFDVKTGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGR
APSASELAAELGMDRAEVIEGLLAGSSYHTLSIDSGGGSDDDARAITDTLGDVDAGLDQIENREVLRPLL
EALPERERTVLVLRFFDSMTQTQIAERVGISQMHVSRLLAKSLARLRDQLE

Molecule Role : Virmugen
Molecule Role Annotation : A sigF mutation is highly attenuated in Mycobacterium tuberculosis and induced protection from challenge in mice(Geiman et al., 2004).
Related Vaccine(s): Mycobacterium tuberculosis DeltasigF mutant vaccine

41. SodA

Gene Name : SodA
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 886174
NCBI Protein GI : 15610982
Locus Tag : Rv3846
Genbank Accession : NC_000962.2
Protein Accession : NC_000962.2
Other Database IDs : EMBL: AE000516
EMBL: AF061030
EMBL: BX842584
EMBL: X52861
Gene3D: G3DSA:3.90.149.10
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
InterPro: IPR001189
KEGG: mtc:MT3960
KEGG: mtu:Rv3846
LinkHub: P17670
PANTHER: PTHR11404
PDB: 1GN2
PDB: 1GN3
PDB: 1GN4
PDB: 1GN6
PDB: 1IDS
Pfam: PF00081
Pfam: PF02777
PIR: S15205
PRINTS: PR01703
ProDom: PD000475
PROSITE: PS00088
TIGR: MT3960
TubercuList: Rv3846
UniProt: P17670
Taxonomy ID : 1773
Gene Starting Position : 4320704
Gene Ending Position : 4321327
Gene Strand (Orientation) : +
Protein Name : SUPEROXIDE DISMUTASE [FE] SODA
Protein pI : 6.43
Protein Weight : 23033.8
Protein Length : 207
Protein Note : Rv3846, (MTCY01A6.22c), len: 207 aa. sodA (alternate gene names: sodB, sod), superoxyde dismutase (EC 1.15.1.1) (see citations below), equivalent to many e.g. P47201|SODM_MYCAV|SODA|SOD from Mycobacterium avium (206 aa), FASTA scores: opt: 1210, E(): 1.8e-73, (82.5% identity in 206 aa overlap); Q9F9R1|SOD from Mycobacterium paratuberculosis (207 aa), FASTA scores: opt: 1207, E(): 2.9e-73, (81.65% identity in 207 aa overlap); O86165|SODM_MYCLP|SODA|SOD from Mycobacterium lepraemurium (206 aa), FASTA scores: opt: 1204, E(): 4.5e-73, (82.05% identity in 206 aa overlap); P13367|SODM_MYCLE|SODA|ML0072 from Mycobacterium leprae (206 aa), FASTA scores: opt: 1169, E(): 9.6e-71, (80.5% identity in 205 aa overlap); etc. Contains PS00088 Manganese and iron superoxide dismutases signature. BELONGS TO THE IRON/MANGANESE SUPEROXIDE DISMUTASE FAMILY. ALTHOUGH FOUND EXTRACELLULARLY, NO SIGNAL SEQUENCE IS PRESENT. AN ALTERNATIVE SECRETORY PATHWAY MAY BE USED.; sodB; sod
Protein Annotation : FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems (UniProt: P17670)

CATALYTIC ACTIVITY: 2 superoxide + 2 H(+) = O(2) + H(2)O(2) (UniProt: P17670)

COFACTOR: Binds 1 iron ion per subunit (UniProt: P17670)

SUBUNIT: Homotetramer (UniProt: P17670)

SUBCELLULAR LOCATION: Secreted protein (UniProt: P17670)

SIMILARITY: Belongs to the iron/manganese superoxide dismutase family (UniProt: P17670)

CAUTION: Although found extracellularly, no signal sequence is present. An alternative secretory pathway may be used (UniProt: P17670)

DNA Sequence : Show Sequence

>GeneID|886174 [Mycobacterium tuberculosis H37Rv ] 4320704..4321327
gtggccgaatacaccttgccagacctggactgggactacggagcactggaaccgcacatctcgggtcaga
tcaacgagcttcaccacagcaagcaccacgccacctacgtaaagggcgccaatgacgccgtcgccaaact
cgaagaggcgcgcgccaaggaagatcactcagcgatcttgctgaacgaaaagaatctagctttcaacctc
gccggccacgtcaatcacaccatctggtggaagaacctgtcgcctaacggtggtgacaagcccaccggcg
aactcgccgcagccatcgccgacgcgttcggttcgttcgacaagttccgtgcgcagttccacgcggccgc
taccaccgtgcaggggtcgggctgggcggcactgggctgggacacactcggcaacaagctgctgatattc
caggtttacgaccaccagacgaacttcccgctaggcattgttccgctgctgctgctcgacatgtgggaac
acgccttctacctgcagtacaagaacgtcaaagtcgactttgccaaggcgttttggaacgtcgtgaactg
ggccgatgtgcagtcacggtatgcggccgcgacctcgcagaccaaggggttgatattcggctga

Protein Sequence : Show Sequence

>gi|15610982|ref|NP_218363.1| SUPEROXIDE DISMUTASE [FE] SODA [Mycobacterium tuberculosis H37Rv ]
MAEYTLPDLDWDYGALEPHISGQINELHHSKHHATYVKGANDAVAKLEEARAKEDHSAILLNEKNLAFNL
AGHVNHTIWWKNLSPNGGDKPTGELAAAIADAFGSFDKFRAQFHAAATTVQGSGWAALGWDTLGNKLLIF
QVYDHQTNFPLGIVPLLLLDMWEHAFYLQYKNVKVDFAKAFWNVVNWADVQSRYAAATSQTKGLIFG

Related Vaccine(s): M. tuberculosis DNA vaccine pAK4-sod

42. trpD

Gene Name : trpD
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 887681
NCBI Protein GI : 15609329
Locus Tag : Rv2192c
Genbank Accession : BX842579
Protein Accession : NP_216708
3D structure: PDB ID : 2BPQ
Taxonomy ID : 83332
Gene Starting Position : 2455630
Gene Ending Position : 2456742
Gene Strand (Orientation) : -
Protein Name : anthranilate phosphoribosyltransferase
Protein pI : 6.4
Protein Weight : 34267.71
Protein Length : 370
Protein Note : Catalyzes the conversion of N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate

DNA Sequence : Show Sequence

>gi|57116681:2455630-2456742 Mycobacterium tuberculosis H37Rv, complete genome
GTCAGATCTGCCGACCGAACCGCACCCATCGCGCGAGCAACTGTTCGGCCGCACCGGTGTCGATTGCCGC
ACTGGCCCGCCGCAACCCCTCCTCCCAGGCCGGCAGCCATTCAGCACGGCTGGATAATCCGGCGTGGGCG
ACGATCGCACCGGCGGCGTTGAGCACCACAGCGTCCCGGACCGGGCCCCTGGCACCGCCCAACACCGCGC
GCACCGCGGCCGCGTTGGCTTGCGCATCGCCTCCAGCCAGCTGGTCAAGCTGGGCGCGCGCAAACCCGAA
TCCGGCGGGATCAAACGTCAACTTATCCACGCTGCCCGCCGCAACGCGCCAGATCGTGCTCGTGGTGGTG
GTGGTCAACTCGTCCAGCCCATCGTCGCCGTGTACCACCAGCACACTGGACCGGCGCGCAGCAAACACCC
CGGCCATCACTTCGGCGAGGTCGGCGAACGCGCATCCGATCAGTCCAGCCCGGGGCCGGGCCGGATTGGT
CAGCGGCCCGAGAAGATTGAACACGGTGGGCACACCGATCTCGCGGCGTACCGCGGCCGCGTGCCGGTAG
GAGGGATGGAACCGCGGCGCGAAGCAGAACCCGATCCCAACCTCCGCGAGGCTGCGCGCGACCAGGTCGG
GTCCCAGGTCGATGCGCACCCCCAGCGCCTCCAGCGTGTCGGCGCCACCGGACAACGAGGACGCCGCTCG
GTTGCCGTGCTTGACCACCGGCACACCCGCAGCCGCCACCACAATCGCCGCCATGGTGGATAGGTTCACC
GTGTTGACTCCGTCGCCACCGGTGCCGACGACGTCGACGGCGTCGTCGGGGACCGTATCGGCGGGCAACG
GATGCGCGTGGCTGAGCATGACGCCAGCGAGCTCACCGACTTCGTCGGCGGTCGGAGCCTTCATCGTCAT
CGCCACCGCGAAGGCGGCGATCTGCGCCGGCCGCGCATTGCCGGTCATGATCTGGTCCATGGCCCAGGCA
GCCTGGCCCCGCGCCAGATCGCGGTTGTCGGTCAACCGCCCCAAAATCTGCGGCCAGGACGGCACCGATG
CGGCTTCTGCTTTCGGCGAGCCCCCGCGAGATCCCCCCGAAGAACCCTCAGCTGACAGCGCCA

Protein Sequence : Show Sequence

>gi|15609329|ref|NP_216708.1| anthranilate phosphoribosyltransferase [Mycobacterium tuberculosis H37Rv]
MALSAEGSSGGSRGGSPKAEAASVPSWPQILGRLTDNRDLARGQAAWAMDQIMTGNARPAQIAAFAVAMT
MKAPTADEVGELAGVMLSHAHPLPADTVPDDAVDVVGTGGDGVNTVNLSTMAAIVVAAAGVPVVKHGNRA
ASSLSGGADTLEALGVRIDLGPDLVARSLAEVGIGFCFAPRFHPSYRHAAAVRREIGVPTVFNLLGPLTN
PARPRAGLIGCAFADLAEVMAGVFAARRSSVLVVHGDDGLDELTTTTTSTIWRVAAGSVDKLTFDPAGFG
FARAQLDQLAGGDAQANAAAVRAVLGGARGPVRDAVVLNAAGAIVAHAGLSSRAEWLPAWEEGLRRASAA
IDTGAAEQLLARWVRFGRQI

Molecule Role : Virmugen
Molecule Role Annotation : A trpD mutant is highly attenuated in mice and induces more protection from challenge with wild type M. tuberculosis than that induced by the current vaccine BCG (Smith et al., 2001).
Related Vaccine(s): Mycobacterium tuberculosis trpD mutant vaccine

III. Vaccine Related Host Genes

1. APOBEC3B

Gene Name : APOBEC3B
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 9582
NCBI Protein GI : 22907025
Locus Tag : CTA-150C2.15-003
Genbank Accession : AL022318
Protein Accession : NP_004891
Taxonomy ID : 9606
Chromosome No : 22
Gene Starting Position : 22346831
Gene Ending Position : 22357253
Protein Name : apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B
Protein pI : 5.94
Protein Weight : 44235.85
Protein Length : 382
Protein Note : Also known as ARP4; ARCD3; PHRBNL; APOBEC1L; bK150C2.2; DJ742C19.2; FLJ21201

DNA Sequence : Show Sequence

>gi|157729478:22346831-22357253 Homo sapiens chromosome 22, alternate assembly HuRef, whole genome shotgun sequence
CACAGAGCTTCAAAAAAAGAGCGGGACAGGGACAAGCGTATCTAAGAGGCTGAACATGAATCCACAGATC
AGGTACCGCTGCCCACTCTGCCTGCTGGGCCCCTCCTGCCCCTTCCTGCCTGGTGGTCCTGCTGGGCCTC
AACCCTGGCCTCCCCCCGCCCCTGCCCCAGCCCTGGGCTCCCTCCCCTCTGGCTCCCCTGCCACACGAAT
CCCAGTCAGGCTCTTTGTCCTGCTGTGTGGTCACCCCACTGCTGCTTCTGAATGGGCTGCCTCCCCTACC
TGCACCAGCCCAGGCCCCGTGCTGAGACTCTCCCTTAAAGTCATGCCCGGACTGGTGCTCCCCGCCCTGG
GAGGGTGCTCCCTCTGTGTGCTTCCTGCCACTCCTGAGCTCAGAAACTGGGAGAATTGAACCAAAGGGTA
ATTGGAGCAATCAGGCATTTTTGTTCTCAGTGTTTTGATGAAATTTCTGTAAGAATTACTTTGCTTAAAT
ACAAAGTCTTAGGAGAGGGTGCGGGGGTAGGAATGGTCTCTGAAGCACATGATAAATCGTGTCATCCAAG
GATGACTCCATGGTGAGCAGATGTCCCCAGACAGGTTCCACTCCAAGGTCACTCTTCATCTTTTAGAACA
TGACCAGTGGTTGAGCGTGGTGGCTCACACCTGTAATCCCAACACTTTGGGAGGCCAAGGCGGGCGGATC
ATGAGGTCGGGAGTTTGAGACCAACCTGGCCCATATGGTGAAACCCCGTCTCTACTAAAAAACATACAAA
AATTAGCCAGGCATGGTGGCGCATCTCTAGTCCCAGATACTCAGGAGGTTTAGGCAGGAGAATTGCTTGA
ACCTGGGAGGTGGAGGTTGCAGTGAGCTGAGATCATGCCACTGCACTCCAGCCTGGGCGACAGAGTGAGA
CTTCATCAAAAAAAAAAAGAAAGAACATGATCAGTAACATGGAATGTGAGAATGTGGGAAAAAAGTGCAA
TGATGAGGAGAAATGCTCTGATTTTAAAATTTAGTGAAAAGAGAGCCCAGTCCCCAACAGCTTTCTCCTA
CAGGATCCGTGATGCAGAGGCTGGACAGGGCTGGCCTGGAAAGGGGCCCCTGCTCCATCCGTCCCCAGCT
CTGTGGCCTCGGCAGGTTACCCCGCCTCTCTGTGCCTCTGGCATCTCCTCTGTAAGATGGGAATGGCCCC
TGCAGGCCTAGGGTAGCCTCACGTGAGCTCACACCCGCTCAGCACTTAGAAGAGTGGCCTGGGCCTCAGA
ATTCAGTTCTACCATGATTTTAAAATAATGTTAACACGCAGAGTGAAACATCAGGAAGGAATTGAGCTGA
AAGGTTAAAAGCTATTTTTTAGGGGTGAAGGGTTTTATTCTCTTTTGCATTCTCCTAATGGGTTGGGGAG
TGGGTTGGCAGAAATACTAGGAGGGTGGGGAATGTACCCCTGGAAAGGCCAGAGTTGGACCCGAGCTGGG
AGAGATCACCCCAGCCCGCCTGCCAGCATCCCCTCCTCTTCCTCTCCCCTCAGTCTTCCTGCCTGGGAAG
GCAGCAGAAATTCTCAGGGCTGTGGAGGGGTCGGGGAGGCCCAGGGCCATCCTGGGAGCTGTGTTCAGTG
GACATGAGCCCCGAGGACTCCCGGGAGGGCTCCCTGCATGGGCCGGTTTCTCTCTTGTGCCTTCAGAAAT
CCGATGGAGCGGATGTATCGAGACACATTCTACGACAACTTTGAAAACGAACCCATCCTCTATGGTCGGA
GCTACACTTGGCTGTGCTATGAAGTGAAAATAAAGAGGGGCCGCTCAAATCTCCTTTGGGACACAGGGGT
CTTTCGAGGCCAGGTACCACCCAAACTTCAATCGAATCACAGGCAGTGTTGCAGGAATTAGAGGACTGGA
GAGACTGATATGGGTGAGACAGGAGGATTTATTTAGGTGCACTGGTTCAGCAGACTCACATCCAAAAAGG
CTGAGCCCTTAACAAAGACAGACTGAGGTATTTATAAGCAAACTTACAGAAGCAGAACAAAGGCGATTAA
TCAAACAGTGACAGGTCACATAATCTATAGCATAACAGATGACTTAGCATAACTTGGGGCCTTCTATAGC
TGGTGGCCTTGCAGCTACATCAAAAGGAAAACAGGAACTGGCTAAATACAGACATTTGTAAAATATAGTT
ATAATGGTTCTGGAAAGGAGAGACAGTAAAGGAATTTGTCTTTTTTTTAAAAAATTTTTTTAAAATTTTC
TTCAACCTTGCCCTGGAGGGGTGTGGGGGTGGTGTCTGGAGCCCATTCCTTTGGCCTTGACTTTTCAGGC
AGTGTTATAACTGTCGTTGGAGTGAGCTGGCTAGGCAGAGGAAAACTTGTTCTTCTTTTCTTTTTAACCC
TTGCTACAGTAGGACCTAAGCAGCTGGAAATGCAGAAAACACAACAATAAGTAAAATGCCCAGCACTGGG
CTCTCAATTGTGTATTTTTCCCACATTTCTTTTTTCTCTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTCC
GAGATGGAGTCTCGCTCTGGCACCCAGGTTGGAGTGCAATTGCACAATCTTGGCTCACTACAACCTCCAC
CTCTCAGGTTCAAGTGATTCTTCGGCCTTAGCCTCCCGAATAGCTGGGATTACAGAAGGCTGCCACCACG
CCCAGTTAATTTTTTCTATTTTTAGTTGAGACTGGGTTTCACCATGTTGGCCAGGCTGGGGAATTCCTGA
CCTCAGGTGAGTCACCTGCCTTGGCCTCCCAAAGTGCTGGGGTTACAAGCATGAGTCACCGCACCTGGCC
ACTTCCCACAGTTCTGTTTTTTTGTTTTGTTTTGTTTTGGTTTTTTTTTAAGACTGAGTTTCACTCTTTG
TTGCCTGGGCTGGAGTGCAATGACTCACTCTCAACTTCCACCTGCCAGGTTCAAGTGATTCTCCTGCCTC
AGCCTGTGAGCTGAACAGTCACATGAGGGTGAAGGTTGTTGCCACAAGCTCAGGGGATGCTCCAGACAGA
GTGGCCTGGGATGTCGAGTCACAGCTGCTCCATGCCACGGGGACAAGAGGAAGCAGGAGTTTAGTCTGAC
ACACTGCCCTTCCAGATAGAGGGCAAGAGACAGAAAGAGGGGCTGAAGTCACCCTTGAATAACCACAGCA
GTGGCACCCACAAAGGTGCAGTCCCCACAGCCTCATAGCCTCTTCTAGGTGCCACCTCTTAAGGCCATTA
CAATAGCCATTAACACTGAACGTGAGCTTTGGAGCAGACAATCACTCAAACTAAACAGGGGGGATGGAGG
AAAGGAGCTTCAATGGCAAGATCCCCTGGGCTCCTGTCCTGGCCCCTCCTCTCCCTGCCCCACCCCTGCA
CTCCTCCTGCTCCCCCTCTCAGAGCATCCCCTGCCCCCTGCTCCTCTCCCAGGTGTATTTCGAGCCTCAG
TACCACGCAGAAATGTGCTTCCTCTCTTGGTTCTGTGGCAACCAGCTGCCTGCTTACAAGTGTTTCCAGA
TCACCTGGTTTGTATCCTGGACCCCCTGCCCGGACTGTGTGGCGAAGCTGGCCGAATTCCTGTCTGAGCA
CCCCAATGTCACCCTGACCATCTCCGCCGCCCGCCTCTACTACTACTGGGAAAGAGATTACCGAAGGGCG
CTCTGCAGGCTGAGTCAGGCAGGAGCCCGCGTGAAGATCATGGACTATGAAGGTGAGAGGTGGAGGGGTC
AGGGGAGCGTGAGCGGGAGGAACAGCATGAAAGATGGATGGATCTGCAATGCCATGGCTGGGGGTGTCCC
AGGGCAGCCTGCAGGGGTGGGACTGGCACTGACTGCAACTGACAGCCAGGAGACCAGGCCTGGGAGGACA
GGCCAGGGTCAGGGGAGAGCCTGACTGCTTCCCGCTTCTTCATCTCAGAATTTGCATACTGCTGGGAAAA
CTTTGTGTACAATGAAGGTCAGCAATTCATGCCTTGGTACAAATTCGATGAAAATTATGCATTCCTGCAC
CGCACGCTAAAGGAGATTCTCAGGTGAGGGTCTCCCTCTGGCCTCATCATCTCTCTCCTCTCACCTGCTC
ATCCTCCTGAGGCCTCCGTTGGCCTGGCCCTCCCGCCCTCCCTCTTGACCCACTGCCTGCCCTCATGGTC
ACACCACCTTCCCTTAACTCCTGGTGCTCCCTCCACACTGCCTCCTCCCTGCTTTCCTGGGCCCTTCCTG
TGAGTAAGAGGCCCCTTCTGCCTCTAAGGCACCTGGGTCTTGGACTAGAGGAACAAACAGCAAGACAGGG
TGGCCTGGGACACCAGCCGCTGCCCTTCTGTGACATGGGGACAAGAGGAGGCAAGAGCTCAGCCTTCCAG
GGAACAACTCTGGGCAGGAGATGTGGAAGGAAGGAGCTCAGTGTGGGGGCACCCATGGCATCCTGGGGGG
ACATCTGAGGGCCACCCCCACCCGCTATTCCTCCCTCCAATGGTGGCCTCTGAGTGTGAAGGCATGGGGG
AAGGCAGACACCTGCCCCTGACTCTCCCTCACTACCACATAGCTGCTGGGTGGCTGGGGACGGGGGGGGT
CCCTGGGATGATTCCTGAGGGCAGGATCCAGGGGTCCCTCCAGATGTCCTCCCATCCAGGTGAGAAAGCA
GCATAAAGTGAGGCTTGCACTCAGATGGGCCTGGGAGGTCACTCACAGCGCAGGTGTCTCCTGGAACAAG
GGCCCTGGAAGATAGAAATAGAATCCAAACCCAGGACTAACATCAGGGCCAAGACAAGCCTGCCAGGGAG
GCTGCACATGAAGCCCCAGATCAGGGACCACTGCCCGCTCTGCCCATGGGGCCTCTGCTGCCCTTTCCTG
CCTGGTGGTCCTGCTGGGCCTCAGCCCTGGCCTCCCCCTGTCCCAGCCCCAGCCCTGGGCTCTCTCCCCT
CCTGTTCCTCTGCCACCCCCACTCCCAGCCAGGCTCTTTGCCCTGTTGTGTGGTCGCCCCACTGCTGCTT
CTGAATGGGCCACCTCCCCCACCTGCCCCAGGCCAGACCCCCTGCTGAGACTCTTCCCTGAAAGTCACCG
CTGGGCTGGTGCTCCCTGCCCTGGGAGGGTGCTCCCTGTATATGCTTCCCACCATTCCTGAGCTCTGGAA
CTGGGAGAATTGAACCAAAGGATGATTGGAGCAATCAGGCATTTTGTTCTTAGCATTTTGATGGAATTTC
TGTAAGATTTACTTTGTTTCAACACAAAGTCTTAGGAGAGGGTGTGGGGGAGGAAATGGTCTCTGTACCA
GAAAATAAGTCATTTCTTCCAAAGACGCCTCCGCTGTGAGCAGAGGAGGATGCACACGACCCCAGACACA
GGCTCCTCCTCCGTGAGCACCATTCACCTTTTAGAATGACACCTGCAACATGGAGTGTAGGAAAACATAT
GATCAGGAGAAATGCTCTTATTTTAGGCAGGGCACGGTGGCTTATGCCTATAATCCTAGCTCTTTGGGAG
GCTGAGGTGGGTGGATCACTTGAGGTCAGGAGTTGGAGATCTGCCTGGCCAGCGTGGCAAAACCTCCTCT
CTACTGAAAATACAAAAAATGAGCCAGGCATGGTAGCGGGAGCCGGTAATCCCAGCTACTTGGGAGGCTG
AAGCAGGAGAATCGCTTGAACCCGGGAGGGACAGCAGTGCTGGTCACGGAAACACCCAGGACTCAGGACT
GAAGCACAAGTGTTTCCAGTCCCCACACACTAAAAAGGAGACAAGAACTCCTCTTCGAGCTTTTCTGATG
AGCACTCACCTCTCACCCTCAAACCCCTGGGGCCTCTCTTTTCCTCCTGACCCTCTCTCTGGACCTGGCC
TCTTGCTCTAGGTTCCCAGTTTTGGTCCCAGTGCTGGTCTCTCCTCCAATGGTACCAATTCCAGGTCTCC
TTCCACCTCCTGGGGCAGATGCACAGGGAGCCTGAAAATCCCAGTGGATAATGATGCTGTGCCCCAGCCA
GTGAAGACAGTTGGAGAGGGGATTCTCTTCACTTGTCTTTAAATGAGCATCTACTATTTCTTGCAAATGT
TCCATGTCTCTGGACTTGGCCTAAGCCATTTTTATGTTCAGTGTCATCTCGTTCTCCCTAATATTTAGAA
AAGGGTGTTGCTTGTAGGACCACTTTGCTATGAAGAAGCCTTGTTTCCTTTTCTTGAGAACACAAAGCAA
GTGAGTGGTAGAGCCCAGATTCATACCCAGGTCTACTAGAAATTGTGGCAAACAGAGATTCCTTCCTTCC
TTCCTTCCTTCCTCCCTTCCTTCCTTCCTTCCTTCCTTGCTTCCTCTCTCTTTCTCTCTTTCTCTCTTTC
TCTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTT
TCTTTCTTTCTTCTCTCTCGTCTTTCTTCTTTTGCTGCTGCCTCCAAACAGAGATTTTTCTAATTCTGGT
TTTGTCAGGATGTCTACAGAGGGGCCGGGAGAGGCCTTCAAGGTGGGGCTGGTGTTTCCAGCCCAGGAGT
CCTGAGCTGCCCCATCTGGCCTGAGAAGCACGTTTGGGAGACCTCATGGCATCCAAAGGGACGTGGAAAA
TGCTATGAATTATCCAAAAAGATGTCCCTGGCAGGCTCCTCTCCCCAATGATCCCCCTCAGGAGTGTCCC
TGCCCCAATGCTGGGTGTGGTAGGAGTTAACCCTGCAGGCAGGAGGAAGCCCCAGCAGGTCCATCTCCAG
CAGGGGCTGTGGGTGAGCAGAGCCAGGACAGGGGTGCATGGTGAGGCCAGGGAAGGAGACCCAGCTCTAC
CCCAGGGAGAGGGGCAGGGTCCTCCCCGGAGGGCCTGAGCACACTGAGCTGACCCTGGGGAGACCCTGAC
AAGGCCTAAACAGGCCCCGGGGCTGTGTTGACTTCCTGATAATCCACCAGAAGGGGTCAGAGAGAGAGGA
TCAGTGGCCCCCACAAAGGGAGTGGAAGCGCCTCCTCTGACAGGTGCCTCAGTATATGGGGAGCAGGGAA
GGAGTGGGGGGTGCAGGAGAGGAGCGAGAGGTAGTCCCAGGAGGAGTCTTAGGGCTTTTGGTTTCCCCTG
TCTTTGTCCACAGATACCTGATGGATCCAGACACATTCACTTTCAACTTTAATAATGACCCTTTGGTCCT
TCGACGGCGCCAGACCTACTTGTGCTATGAGGTGGAGCGCCTGGACAATGGCACCTGGGTCCTGATGGAC
CAGCACATGGGCTTTCTATGCAACGAGGTGACCGACCCAGCCACCTGCATCCAGGCAGGGCCCTCCCAAT
CCAGGGACACTCATAGATAGAAGGTTCTGGGTGGTGCCTGTGGTTTCCTGCAGTGTTTGTCACTTGTGCT
TCCTGCAGCTGCTGCTGCTTGGCCCTGGAGTTGGGGGGAGACTTTGGCTTCAGTGACTCTCCATGGCCAG
GTGGGGTTGACTCTGCCCAATGGCAAAGTGCTTCCTGAGGACCCTCCCAGGATCCCCTCACAGACACAGC
TCTCACCAGGAATGATTCGGAACTGTGGGATTTGAGGACTCAGGGCCTACCTGACTCACAAGGCCAGGAT
GTCCCTGTGCCCTCTCTTGGGCTTCATCCTGCGGAGAGACAGACTGGGGCAGGAGAGGCTGATCAGGGGT
TTTGTCCTGCCCAGGGTGGAGCCCACAGCAAGGCCAGAACAGGTCCCATGTCAGGATGCAAAGATGGCCA
GCAGTTGGGGAAGAACTGGGCCAGGAAAGGCTGAGGGGGCCAGGAAAGGCTGAGGGGGCCAGGCTAGGCT
GGCCAGGAGCCCTGAGCCCAAGGGACTTTCTTCCCTGGCCCCTACCCAGCACAGCCTCTGTCTGGAGAGG
CCAAGTTCTGCTTGGCTCTGTTGCTGGAAACTGGGGGCTTCTCTGGGCTCTATGAGCTCAATGTGGGCCT
CGCTGGATCCACACACACTGCTTGGAACATCCTTTCAAGGGTGCCAGTCTCCATCACCGCCTAAGCAGTG
GGACTCCCCCAGGAATGACCCACAGCCCCTTCTCAGCACAAACCATGTCATCCACTTCACCCTGCATCCC
CTCTGATGGAATCCCTCCCTGTCTTTGTCCCCATCACAATCACAGTCCTTGCTGCCCTGCTGTCCCCAAC
TTGACCGATTCCTAGTCTTAGTGGAAAATCCTTTTCTCTGAAGTTTGGAGTAAATACCATATTTCTATAA
GTCAAAATAATGACAATTATACCATGTCACAGGGACCTTCCCACATTGGAGTGAGTCCTGCTGGTCTTCT
CTTCCCCACTACTTTTGGTGGAATTCATTTTGGCCTAAGTCTATATCACATTTGGGCTCAGCACAGGTTG
TGGCACAGAATAGAAGTTTCTAGAATTATGGGCCACAGATGGGATGGGAGTAGGGGAGTTGATGAGTAGA
GCAGGTCAATCTCCCCTTGAAGGAAGCACACTCACTCAGGGGCATCAGGCCAATGACCTTGGGGCTCCGC
CGGCCCCTCCTCCCTGCCCCCATCTCTGTAGCCCCTCCCTGCAGGGCTGGGTCTGGGGTGAGGGTCCTGA
AGGCTCTGACCTTGGGTACAAAATTGATGGGGGCTCCAAATGCTCAGTAATTAGAGAAAACACATTTTAA
TTTAATAATTAATGCAATATTTTAAAACGCAAATTTAATACAAATAGATCATGATGAATAAAAGCAAAGA
TTTTAATAAAGCCTGGCAGGAGAGTGCTGTCCTGTGTATATTGGAGCTGGGGCAAAATAAAACCTCATTG
CTCCTGATCTGTTTGTATTTAAATGGGCAACATTTTGCTTATCTTGGGATTTTTGCATAACATCTGATAT
TTTTAATATTGCATAGAAAATCATGCATCCTAATAACCGGGGTTTTGGCGACCCCTCTAATTTCGTGCTG
GAGGCCCCCGCCTCCCCAGCCCCACCTGGTCCAGGCGCTCCCTCCCTGTTCACCACACATCACCTCACAC
TCTGTTTCCTTTTCTAGGCTAAGAATCTTCTCTGTGGCTTTTACGGCCGCCATGCGGAGCTGCGCTTCTT
GGACCTGGTTCCTTCTTTGCAGTTGGACCCGGCCCAGATCTACAGGGTCACTTGGTTCATCTCCTGGAGC
CCCTGCTTCTCCTGGGGCTGTGCCGGGGAAGTGCGTGCGTTCCTTCAGGAGAACACACACGTGAGACTGC
GCATCTTCGCTGCCCGCATCTATGATTACGACCCCCTATATAAGGAGGCGCTGCAAATGCTGCGGGATGC
TGGGGCCCAAGTCTCCATCATGACCTACGATGGTAAGAATGGAAGGTTCAGGTGGGGTGGGGTGGGTGGG
GGCAGGAGAGGTTCCTGGGAAGAAAAGGAGAAAGGCCTTGGTCTGCTGCCTGCAGAAACGATGGCTGGAC
TCTGGGACCTGACTTTGGGGTCGATGGGAAGAGAGAGGCCAGGCCAGGAGATATGGGCCCGGGGAGGGTG
GCTGGAAGTGGAAGCAGAACTTGGGGATTTCCGAAAGAAAGAGAACTGGGCTGGCCCAGATTCCAATGGG
AAGGAAGTACCTGATGAAGGAGCTAAGTCCCTAGGGGAGGGAGAGGGAAAGGAGGGACTGAAACCAGGAT
GTGGGAAGTCTGTCCTGAGAGTCATGGGCCCTTGGTGCTGCCCCCTCCCCACAACAGGAGCGTGACTTAT
CTCCCCTGTCCCTTTTCAGAGTTTGAGTACTGCTGGGACACCTTTGTGTACCGCCAGGGATGTCCCTTCC
AGCCCTGGGATGGACTAGAGGAGCACAGCCAAGCCCTGAGTGGGAGGCTGCGGGCCATTCTCCAGGTGAG
GGCTTCCTCCCTCTGCCTGGTGCCCCATCGGCCTCCCCCTCCTCCCCGCTCCCCTGTGCCTTGCCTTCCC
CTCTGCTCAGAGCCTCCTCTGGGTTCCCTGCTCCCCACAGGGCGCCCAGCTCCGTCCCTCCCTTTCCTTC
TCACAGCCTCCTTCTCTTTCCCACCTCCCGCATCCCTCCCTCCTCTCCCGTCATTGTCACTGTCCCCAGG
CCACCTCCCTGTGCCCTCTTTCCACTCTCTCACCTCCTGCTCCATTCAACCCCCCTGCTCTTCCAGAATC
AGGGAAACTGAAGGATGGGCCTCAGTCTCTAAGGAAGGCAGAGACCTGGGTTGAGCAGCAGAATAAAAGA
TCTTCTTCCAAGAAATGCAAACAGACCGTTCACCACCATCTCCAGCTGCTCACAGACACCAGCAAAGCAA
TGTGCTCCTGATCAAGTAGATTTTTTAAAAATCAGAGTCAATTAATTTTAATTGAAAATTTCTCTTATGT
TCCAAGTGTACAAGAGTAAGATTATGCTCAATATTCCCAGAATAGTTTTCAATGTATTAATGAAGTGATT
AATTGGCTCCATATTTAGACTAATAAAACATTAAGAATCTTCCATAATTGTTTCCACAAACAC

Protein Sequence : Show Sequence

>gi|22907025|ref|NP_004891.3| probable DNA dC->dU-editing enzyme APOBEC-3B [Homo sapiens]
MNPQIRNPMERMYRDTFYDNFENEPILYGRSYTWLCYEVKIKRGRSNLLWDTGVFRGQVYFKPQYHAEMC
FLSWFCGNQLPAYKCFQITWFVSWTPCPDCVAKLAEFLSEHPNVTLTISAARLYYYWERDYRRALCRLSQ
AGARVTIMDYEEFAYCWENFVYNEGQQFMPWYKFDENYAFLHRTLKEILRYLMDPDTFTFNFNNDPLVLR
RRQTYLCYEVERLDNGTWVLMDQHMGFLCNEAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFIS
WSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFEYCWDTFVY
RQGCPFQPWDGLEEHSQALSGRLRAILQNQGN

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

2. C19orf66

Gene Name : C19orf66
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 55337
NCBI Protein GI : 154350198
Genbank Accession : AC020931
Protein Accession : NP_060851
Taxonomy ID : 9606
Chromosome No : 19
Gene Starting Position : 10196805
Gene Ending Position : 10203927
Gene Strand (Orientation) : +
Protein Name : chromosome 19 open reading frame 66
Protein pI : 7.28
Protein Weight : 31422.73
Protein Length : 291
Protein Note : Also known as FLJ11286; FLJ40743

DNA Sequence : Show Sequence

>gi|224589810:10196805-10203927 Homo sapiens chromosome 19, GRCh37.p5 Primary Assembly
CGACTGCAGAGGCCGCGCCGCCTCCCCCGTCCGAGGTCTGCGCGCTCCGCCGCAGGGTGCAGACCCGGGG
CGCCCGCCTGGGTTTGGGGCGCAAGAGCAGAGGCGGAGCCAGGGCGGAGCCAGCGCGCCGGGTCCCCCCT
GAATCGAAAGCGAAACAGGGGCCGGGGAGGAAGGGCGGAGCCGGCCCCGAGGCACCGCCCCCTGCCCTGC
GCGGCTGCTGGACCGACGGGCGCACCCAGGTAGGGGGGCGGCTGAGCCGCGCAGTGCGGACCCTCGCGGG
GAACTGCGCCGCCGCCACCATGTCTCAGGAAGGTGTGGAGGTAAGCGATGGCTACGGCTGGGCCGGGGTC
AGCCGCGACAGACCCCGAGGAGCGGCCGGGAGGCGCGGAGGGGGCTTCGCAGTTCCTGGGGACCCCCATC
CTAGAACCCCAGATCCTTACCCCTGCCTGGCGCTCGCCCCCCTTGAAAAGGAAAGTGACTCCCACGTTTC
CCCAGAGCGAAATGAGGCCTCCCCGAGGAAAAGCGGGGGCTGCAGGGTCAAAGGTCAGAGGCCAGAGATA
ACCTGGCCGCCCCCCCACACCTTAGGCTGGGACGCTCGCCCCAGTCCGCGCCTTCCCCCAACGCCCTGTG
GGGACTTTGGCTTTTTCCAGGAAATGCCGTAAAGGGATGAAAGGCGGGGGGGGGGCGGCGGAGGCCAAAA
CCAAGGGTCAAGTTCGGGCCGGGAGAACGGTGCCTAGAGATGGGGGAGGGATGATCCCGTTTCCCCTTCC
CCCACCGGAACCCCCCTGTCTCCATCCCAGCTGGAGAAGAGCGTCCGGCGCCTCCGGGAGAAGTTTCATG
GGAAGGTATCCTCCAAGAAGGCGGGGGCTCTGATGAGGAAATTCGGCAGCGACCACACGGGAGTGGGGCG
CTCCATCGTGTACGGTGAGGCTGCAGGGGTCCCGGGCCTGGTGCGGGGACCGCGCGTGGGAGCGCCGAGG
GGGCGTGGTCTAGGGAGAGGCGTTGAGATCACCTCCCCCGGAGGTCCCAGCCTTGGGGAGGCTCTGCGTC
GCGGCTCTGGGTGGCCTGGAACTCCCACAGACCCTTCAAGGGCCCTTCCCTTATATGCACTCTCCCCCGC
AGGGGTAAAGCAAAAAGATGGCCAAGAACTAAGTAACGATCTGGATGCCCAGGTAACCTATCCCCTCCCC
TCGCAAAGGACCGGGTCACGGGAGGGAGAGCAAGAGGGGGGACCCGACCCGTTCATGGTGACTGACCCCC
CTCTGAAACAGGAGGGACCAGGCATTGTCGGTCCATAACTCATCACCCAATCTCACTTTTTCACCTCTCC
ATCATTTGTCCGGCCAGGGAGCCTGCAAACAGTAGGCGCTCATTATGTGCTGATTGTATTCAATGGGGGA
GAGAGCCATGAAACTAAGCAATGAAAGGAGCCAGGATTATTGCCATCATAGCGGGGGCGCAGAGGTCAGA
GAAGGCTTACTGGAGGAGGAGGTACCAAGTCTTAAAGGATGGGGAGGAGTGAGCCCTGGGAGGGGAAAGG
GAAAGAAAGGCAGGGGTCTTGGGGTGGGGGAGGGAATGGCATAAGCAAAGGCTGGGAGGTGGGGAAGGGT
TGTTAGTGGCAGTCTCTAGAAAGGCTAGGATGAGCAGAGGATTGGGGAGGAGAGAGAGGCAGGGGAATGG
AACCCTTGTGTTGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGGCTTTGAAGCACAGGTG
CATCATGGCTACTTTGCTTCTTTGACAGCTTTGGTGATGGGAACGCTGTGGATTTAGCCTGCCTGAAAGG
CTTAGTGTAGGGCTGTTAAAGAGTATAATAGGCCAGGCGCGGTGGCTCACGCCTGCAATCCCAACATTTT
GGGAGGCCGAGGCGGGCGGATCACCTGAGGTCGGGAGTTTGAGACCAGCCTGACCAACATGGAGAAACCC
GTCTCTACTAAAAAATACAAAATTAGCTGGGCATGGTGGCGCATGCCTGTAATCCCAGCTACTCGGGAGG
CTGAGGCAGGAGAATCACTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCTGAGATCACACCATTGCACTC
CAGCCTGGGCAACAAGAGCGAAACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAATGCCGGGCGCGGTGGC
TCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGGATCATGAGGTCAGGAGATCGAGACCAT
CCTGGCTAACATGGTGAAACCCCATCTATACTAAAAATACGAAAAAAAAAATTAGCCAGTTGTGCTGGCA
GGCCCCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTG
CAGTGAGCCGAGATCGCACCACTGCACTCCAGCCTGGGCAACAGAGCGATTCTCCGTCTCAAACAAACAA
AAACAAACAAAACCAGAGTATAACAAAACATGTATCATAGTCTACATTTATACATATAAAAGTATGATAA
AAATGATAGCAGAGGGGCCAGGTGTGGTGGCTCACGCCTGTAATTCCAGCACTTTGGGAGGTTGAGGCGG
GCAGATCACTTGAGGTCAGGAGTTCGAGATTAGCCTGGCCAACACGGTGAAACCCCTTCTCTATTAAAAA
TACAAAAAATTAGCTGGGCGTGGCAGTGCATGCTTGTAATCCCAGCTACTTGGCAGGCTAAGGCAGAAGA
ATCACTTGAACCTGGGAGGCAGAGGTTGCAGTCAGCCAAGATTGTGCCATTGCACTCTAGCCTGGGAGAC
AAGAGCGAAACTCCGTCTCAAAAATATAATAATAATAATAATAATAATAATAATAATAATAATATTAGCA
GAGAGTAGCATTCATTGAGTACTTCCCACATGGCAAGATTCCAGGAATTTATTTATTTCATCACTGCAGT
GACCCCAGGAGACAGGTCCTATTATTTATCCCCATTTCACAGACAAAGAAACTGAGGCCGAAGTCACTTG
CCAGCAAGCAGGGGCAGCTGCGATTTGAACCCTGGCCACCTGGAGTCAGAGCCTGGGCTCTAACATGCCT
GTCAGTGGGAACCCAGGGGGCAGGGAGTGGCTTGCCCAAGTCCCCACAGCTGGAAGTGATACAGCTGGGA
TTTGAACCCACATCTCTCTCCCACTTAGCCGAGCACAACGTGGCCTCAAAGGGGCGATATGTCACAACAT
CAACAAGCATGTGGCATTTGCAGGCAGGAAGTGAGTATGGGGGTCAGGCTAGCCTCACTCAGGCAAGCCC
TCCCCTAAGCCTCAGTTTCCCCATCTGAGCAATAAGGTAACAGGTGTGCCCACCTCTCAGGGCTGCTATG
AGGACAACTGAGGCCACACACCTGGCTCTCAATAAGTGAGTGGTGCTCTGTGAGCTTCTGGCGAGTTAGG
GGCACCCTACTCATTGCGGCCGGGGGCCTCCCCCAGCAAACGGGTGGAAAGAGGCCTGAGCCCCATCCCC
AGTTTCTGCCCCTGCTATCTCCACCCAGGATCCACCAGAAGATATGAAGCAGGACCGGGACATTCAGGTG
AGTTGGTGGCTAGGGCTTGGGATGGGGGAGTTGGGAGGGGGACATCTGCAGGCACAGAAGGATGTCCATT
AGGGCAGGAGGGGAGATATTCACTGGGGCAGGAAGAGGACTCGTCTGTAGGGAGAGGAGGGCTGATGCTT
ATGTTGGTGCAGAAGGGGTGACACCCCCCCACCTCATCCCCACCTGCCCCATTCCACAGGCAGTGGCGAC
CTCCCTCCTGCCACTGACAGAAGCCAACCTACGCATGTTTCAACGTGCCCAGGACGACCTTATCCCTGCT
GTGGACCGGCAGTTTGCCTGCTCCTCCTGCGACCACGTCTGGTGGCGCCGCGTGCCCCAGCGGAAGGAGG
TGATGACCTAAAACTTAACCTCGACTTTGACTGTCCCGAACTCCACTCCTGACTCCTATCCTCAGTGACT
GTACCTCTGACCTAAGGATTTCAATCACTGAGAGTCCAATCCCTGACCTCCAAACTCAGAGCTCAGCCTT
GATCTCTGATCCCAACCCCTGACCCAGGACTGGACTTCTGACCTTAAATGTGAGAAGCCCAATACCTCCT
TACCCCAGAACCTGACCCTTGACCCACAATTGAACAGCTACTACAGAAATCAAACCTGGCCAGGCGCAGT
GGCTCATGGCTGCAATCTCAGTGTTTTGGGAGGCTAAGTAGGGAGGATCGCTTGAATTTAGGAGTTTGAG
ACCAGCCTGGGTGACAGGGTGAGACCCTGTCTCCAAAAAAAAATGTGTTTTGAAGACAGGGGGTCTCGCC
CTGTCACCCAGGCTGGAGTGCAGTGGCGGGATCATAGCTCACTGCAGCCTCCACCTCCTGGCCTCAGGTG
ATCCTCCCACCTCAACCTCCCAAGCAGCTGGGACTACAGGCCTGCGCCACCACACTTGGCTAATTTTTGT
ATTTTGTGTGTGTGTGTGGAGATGGGGTTTTGCCATGTTGCCCAAGCTGGTCTCAAACTCCTGGGCTCAA
GCAATCTACCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGTGCCTGGCAAAAAAAA
ATTTTTTTAATTAGCTGGGAGTGGTGGTGTGTGCCTGTGGTCCCAACTACTCAGGAGGCTGAGGTGGGAG
GATCGTTTGAGCCTGGGAGGTCGAGGCTGCAGTAAGCCATGATCACAACACTGCATTCCAGCCTGGGTGA
CAGAGCAAGACTCTGTCTCTAAAAAAAAAAAGAAAGAAAGAAAAGAAACCAAATCTGACCTCAAAAACAC
AGCCCCAAATCTGGCTTCTGAACCTCCTGACTAGTAGTTCACTTCTGGTCAATGCTTAACTAGTTCTTGC
AAAAGAGGCAGGAAGCCAAGATGTGTAGTGTTTGCCAATTTCTGTGGTGTAAATATTCCCACCATGGTCA
ATATCAGGCTACCCACGTGAAGTCACTGATTGAGTTGGGTTGCTCAAGCTGAGGCCAGCCGCTGCAGCAC
ACTGCTAGCCCTGACCCCAACCTCAGACACCTCTGACAATCCTTGGTCCCCTCTCTGTACCTTCCTGCCC
ACCACCACCAGGTATCCCGGTGCCGGAAATGCCGGAAGCGCTACGAGCCAGTGCCAGCTGACAAGATGTG
GGGCCTGGCTGAGTTCCACTGCCCGAAGTGTCGGCACAACTTCCGGTGAGGGCGCTGACCCCCAGCTCCC
CCTCAGCCCTGCCCTACCCCAGCCCTGCCCCTCCCTGCCCTGGCCCCACCCTGGCCCAGCCTCGCCCTCG
GACCCTCACAGCCCTGCCCGCCCCCAGGGGCTGGGCACAGATGGGGTCCCCGTCCCCCTGCTACGGGTGC
GGCTTCCCCGTGTATCCAACACGGATCCTCCCCCCGCGCTGGGACCGGGACCCGGATCGCCGCAGCACCC
ACACTCACTCCTGCTCAGCTGCCGACTGCTACAACCGGCGAGGTGAGGCTCTTCTCCCCCAACAGCCTGG
ACAGTCTTTGTCCCCTTCTGTGCCTTTAAGTCCCCAAATCTCCACTCAGTCCACTTTGTCTCCCACAGCA
GCAGCCACTGCTTCCACATGGCCTCCATGACCCCCCAGTCTCCGTGGTCTTGCCCAGGAGGCTCTGAGGT
TTCACCCCAGTGGCCCGTGCCTGAGGGTCCCCATGGCCTCTGCCCCCATGGTCTCTGGGTTTGGGGCCCC
TGTTTTGTCCCCTGTAATCTCAGGTGACCCCCATGTCCCCAGGTCTCCTGTATCTTCAACACCCCTGAAT
GTCCAGTTGTGCTGGTCCCCGTATCTGGTGGTCTCAGCTCCTCCCTAGAGCCCCGCGTGGCCTAAGTCCC
CTCCTCCCCAGACTCTCATGGGACCTCCAGCCCCATGTCTGCAGCACCTCTCCCTCCCTAGAGCCCCACG
TGCCTGGGACATCCTGTGCTCACCCCAAGAGCCGGAAGCAGAACCACCTGCCCAAAGTGCTCCACCCCAG
CAACCCTCACATTAGCAGTGGCTCCACTGTGGCCACCTGCTTGAGCCAGGGTGGCCTCCTGGAAGACCTG
GACAACCTCATCCTGGAGGACCTGAAGGAGGAGGAGGAGGAAGAGGAGGAGGTGGAGGACGAGGAGGGCG
GGCCCAGGGAGTGACCCCTGCCAGGTGCAGATACAAACCAGACACGGTCTGTGGCTACTTTGTGTTATTA
TAAGATATGAGCTCAAACCGAGATATGAATGACCTTGGGGAGCCATCTGAGGCCAAGATATTGACGGGGG
GGATTCCTGGGTCCCATTTTCAGCGCCCAGGGTCACAGATCCACAGTGGGAAGTTCTGTGGGACACATTG
GCACTGAGCCACAAAGAAGGTGTGGCCAGAACAACTTGGGCTCCTGCTGACCAATGTCCTCTAGGGCCTA
GGGGACAGAGGAACACAGAGTCACAGCTTCAGGGGCCGAATGAGCATGGCGGCCTTCCTGAGAGAATATG
CCCCACCACGAAACTCAGCCCAGTAGACACCATCCTGGTAGCGGCTTCGGTAGTGGCCGCCGTGGTGCCA
CACACCGTTGAGGTTGGAGTGGGCACAGGCATGGTACCACCAGCCTCCCCGCTGGTACAGGGCACAGTTA
CCTGAGGGGAGAGAGAGAGTCCATGTCCTCTCACCAGAATAAAAGCCTCTACCTGCACCTCACAGTGCAA
GGCTTTTGCCAGGCATCCCCTGGCCCCTCCCATTCTTATTGAATACAAGCCCTGATCTTCCATCTCCTCA
GCAAAAAAATAGGAGCCCTGGCCCCCCAACTTTCTTCAGAGTAATAGCCTTAATTCCTTCCCTATCTCCT
TACCAAAGTACAAGTCACATCTTTCCCACCTTTTCTGCAAACTAGGAGTCTACCGTTCATTCCTTTATCA
AAGAAAAGTATCTACTTCCTTTCTAGAATAAGAGTACTAGCTCTCACCCTCTGCCCTTTACTTGAACAGG
AGTCTTGATTCTTTTTTTGCCTCATCAGAGAAGGAATCTGGACTCCCCATCCCCCCACCAGGATAAAAGT
CCTGACCTTTGTTCTCTTGACGGAATAAAAGCTTGCTTATCCTTATACTTACC

Protein Sequence : Show Sequence

>gi|154350198|ref|NP_060851.2| UPF0515 protein C19orf66 [Homo sapiens]
MSQEGVELEKSVRRLREKFHGKVSSKKAGALMRKFGSDHTGVGRSIVYGVKQKDGQELSNDLDAQDPPED
MKQDRDIQAVATSLLPLTEANLRMFQRAQDDLIPAVDRQFACSSCDHVWWRRVPQRKEVSRCRKCRKRYE
PVPADKMWGLAEFHCPKCRHNFRGWAQMGSPSPCYGCGFPVYPTRILPPRWDRDPDRRSTHTHSCSAADC
YNRREPHVPGTSCAHPKSRKQNHLPKVLHPSNPHISSGSTVATCLSQGGLLEDLDNLILEDLKEEEEEEE
EVEDEEGGPRE

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

3. CD70

Gene Name : CD70
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 970
NCBI Protein GI : 4507605
Genbank Accession : AC008760
Protein Accession : NP_001243
Taxonomy ID : 9606
Chromosome No : 19
Gene Starting Position : 6346586
Gene Ending Position : 6351901
Gene Strand (Orientation) : -
Protein Name : CD70 molecule
Protein pI : 8.6
Protein Weight : 19828.21
Protein Length : 193
Protein Note : Also known as CD27L; CD27LG; TNFSF7

DNA Sequence : Show Sequence

>gi|157718668:6346586-6351901 Homo sapiens chromosome 19, alternate assembly HuRef, whole genome shotgun sequence
CTGATTTTTGTATTTTTTAATAGGAAAATGAAAGAAAAAGCTCAATGCCTTCTCTTGTCCTGCCACCACT
ACCCCCCACCACACTCCCACCCCAACCCCGGGTGGCCCCTGTGTGTACACTTTTTCTCTTGAACTTAAAT
AAAATAAAATAAAATTTAAAAAACCCTAATCAGCAGCAGTGGTCAGGGGCGCACCCACTGCACTCCAAAG
AAGGTCTCATCAGTGTTTCGGGAAGGCAAAAGTGTCCCAGTGAGGTTGGTGCAGAGTGTGTCCCCTCGGG
CCAGGGGCGTCAGGCGCTGGGAGGCAATGGTACAACCTTGGTGGAAGCTGAGACGCAGCAGGCTGATGCT
ACGGGAGGCGGGAGAGCAGATTCCCACGGCCAGGGTGGTGGGGTGGTGCCTGGAGGCCGTCGTGGAGGAG
CAGATGGCCAGCGTCACCTGGATGTGTACCATGTAGATGCCATCACGATGGATACGTAGCTGCCCCTTGT
CCAGCTCTGGTCCATGCAGGAAGGAGCGGCCCAGTGCTGGGCCCCCCTGCCAGTATAGCCTGGGGTCCTG
CTGAGGTCCTGGGGGCACAGGGTTAGAGGGATGAGAGGATGGAGGTTTAGGGAAACTGAGGCACAGGGGG
TCATAGAGAAAGTCAGATATAGAGATCGAGAGTTAGAGATCAAGGAATAGGTTGGACGTGGTGGCTCATG
CCTGAAATCCTAACAGTTTAGGAGGCCAAGGCGGGTGGATCACCTGAGGTCAGCAGTTCAAGACCAGCCT
GGACAACAGGGCGAGACCCCATCTCTACAAAAAATAAAAAGTCAGCCAGGTGTGGTGGTGCACGCCTCTG
GTCCTGGCTACTCTTGGGAGGCTGAGGCTGGAGGATCGCTTGAGCCTGGGAGGCTGAGACTGCAGTGAGC
TATGATCACACCACTGCACTCCAGCCTTGGTCACAGAAACCCTGTCTCAAAAAAAAGGGAGGGGGATAGA
AGGTTAGAGATCTAAAGGGAAGGAGAGAGGGGTAAGGGGAAAGGGGTAGAGACAGGGAGAGAGAGACACC
AGGAGAGAGAGAGACAAAAAAAAAAAAAAAAAAAAAACAGAAACAGACAAGGGTAAAGATAGAGGGGGAG
GGAGAGATATATAGAGAGAGAATGCAGAGAGAGAGCAAGAGAGAGAGTGTATGTGTGAGAGAGGACGAGA
GTGCACGAGAGAGTGCGACAGAGAGCATGAGAGAGAGAGCATGAGAGAGCGAGAGAGTGTGTGAGAGGAC
GAGAGAGCTTGAGGGAGAGTGCACGAGAGAGTGCGAGAGAGCACGAGAGAGCAGGAGAGAGTGCAAGAGA
CAGCATGAGAGAGAGAGAGATCATGAGGGAGAGAGAGCATGAGACAGCGAGAGAGTGTGAGAGAGAGGAC
GAGAGAGCTTGAGGGAGAGTGCACGAGAGAGTGAGAGAGAGCACGAGAGAGCAGGAGAGAGTACGAGAGA
CAGCATGAGAGAGAGAGCAAGAGAGAGAGTGTGTGTGTGAGAGAGGACGAGAGAGCTTGAGAGAGAGTGC
ACGAGACAGAGTGTGAGAGTGAGCTCGAGAGAGTGCATGGGAGAGTGTGTGTGTGAGAGAGGATGAGAGC
TACAGAGCTCGAGAGTGCACGGGAGTGAGCGAGAGAGTGCCTGAGAGAGTGAGACAGACAGCACGAGAGA
GACAGCGTGCGCACGAGACAGCGCGCAAGAGAGTGCACGAGAGAATTCCCCAAGGGACGCCTGCTCGGGT
CACACCCTGAACGCCAATAAAAGCTTTGGCTCCCAGGACCCTTTTTTTTTTCTTGCTTTCCACCCGCTGG
TTGAGTGTGTGTCTCAGAGAGCTCCCCTACCTTCCCGTTGGCCCTCTTCTCTCTAGGATCTTTATTTTCT
TTAGAGACAGGGTCTGGTCCTGTCACCCAGGCTGGAGTGCAGTGGTGCAATCATAGCTCACTGCAGCCTC
AGCCTCTGGGGCTCAAGCTATCTCCCACCTCAGCCTTCCCGAGTCACTGGGAGGTGGCATGCGCCACCAT
GACCAGCTAATTTTCAAAATTTTGTTCTAGAGATGGTGGTCTTGCTATGTTTCCCAAGAAACATAGTCTA
GAACTCCTGGGCTCAAGCGATCCTCCCACCTCAGCCTCCCAAAGCGCTGGGATCACAGCCGGGAGCCAAG
GTCCTCGGCTATATTTCACGTGTTTCATCTCAGCGTCTCCTGTCTCCGCAACTTATCTGACTGACACACC
TGAGCCTCACTCTCCTCCTCACTCAAGGCTATAGTGGAGACTGGCTATCTTGTTAGGAAGGAACTGACAC
GGGGTCAAACAGGAGCCCCAAGGGTGTTTACCAGGCTTTGCAAATTTCCTGTAAGAGTGACACCTGGTCC
CCTGTCAGACACTTAGGCATGATATCATCCACCAGAAGAAAGAACTGTCCTGTGAAAGACACTGTCAACA
CCTGTGGCCACCACCCCTGGTGCCCCATCGATGTGGGGCTGGACAGCCACTCTCCAGAAAGGGGCCTCAA
GACCAAATTACAGGAAAAATATGGCAAAGGCCGGGGAGGGGGGGAAGATATATCAGGGGTCAGAGACCCA
AGGAGAAGAGAGAGCAACAAAGGTCCCTTCCATTACAAAATGGAAGGAACAAAACACGCAAGCGTGCAGT
AATTTTAGTCTCTGGGGATGAACCGTGGAAAAACAGAAGAGGAAGAAGCGTTCGAGAGAGGCTGGGAGGA
GGTAGAGACTTCCACCTAGAGCAGAACTGTTCATTTCTTCTCCCTCAAGGCTTCTTGCCAATGCCACCCA
CTCAGATACCACCCCCCCACCCGACCGCCGCGACTCCACTTTAAATGAAAATCCCCAACCCTGGCATGCC
CGGATTCCCCACCCACCTCCTTGCCTGATTTTTTCTCTAAAACCTGCTCTTGGCCTGTTTTACTCTACGT
GGCATTGCTCTGTTTTTCACATGTTCGTTTTTATTTTTTTGGTCATCTCCTCCTCGGTAGGAGTGATAGT
AGAAGGGAAGCTCACCAGGCAGGGATTTCTGTCTGTTTTGTGCGCGACTGCAGTACCGGAACAGTGCCTG
CCCACTGCAGATGTATAATTAGTGCTTGTAGCATGAATGGAGGAAGGGTAGCCTTTCCTGTGAGCGTTTT
CGTTTACTAACAAAACCCCTTTGATTCTTCCAAGGGAGGAGATCGAATGTGAAGGACTGAGAGGGAAAGG
GAGAGAAAGGTAGTGGATGCCCAGAAACACAAACCTGCTCGCTCGCTCTCTCCTCTCTCTCTCTCTCGTT
CTCTCCCTTTTCCTTTTTCTCTTGGGAATGATCTCTTTCTCCCCCGCCCTGTTTTTCTTCTTCCCCCTCT
CTGTCTCTGTCTCTTCCTCTCTGTTATTCTCCTTCTCTTTTCCTCTCTCTGGCTTTCTTTCTTTTCTTTC
TCTTATTTCTTTTTTTCTCTTTCTTCTTTCTTTTTCCTTTCTCTCTCTCTCACCTCCCTCCCTCTCTCCT
TCCTTCCTTCTTCTCTTTCTTTTCCTTCCTTCCTTTCTTTCTTTTTCTTTTCCTTTCTTCTTTTTATTTA
TTTATTTGACAGAATCTCCCTCTGTCACCCAGGCTGGAGAGCAGTGCCATTATCTTGGCTCACTGCAACT
TCTGCCTCCCAGGTTCAGGCGATTCTCCTGCCTCGGCCTCCTGAGTAGCTGGGATTACAGGTGCCTGCCA
CCACACCCGGCTAATTTTTGTAATTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAA
CTCCTGGGCTCAAGTGATCCACCCGCCGCGCCCTCCGAAAGCGCTGGGATTACAGGCTCCAGCAACCACG
CCAGGTCGCCTCTCTCGTTTTTATTCTCTCTTCCTCTATCTGTCTTTTTTCTCTTTCTCCGTCTCTCTCC
CTCTCTCTCGTTCTGTCTCCCCCGCCTGTCTCCCTCCCTCCCTCTTCCACTCTCCCTGCGTCTCTCCCTG
TTTCTTTCTTTCCCCTTCTTCTCCTGTCTCCGTCTGTCCCCTCTGTCCCTCTCTCTGTTCTTTTTTCTGT
CTCTCCCTCCCTTTCTCTGGGCCTCTCCCTCCCTCTCTGTCTTCTGTCTCCCCCTCTCCGTTTCCCTCCC
TATCTCTCCCTCCCTCTCTCCCTCCGTCTCTGTCTGTGTCTCTTTCTCTCTGTCTCTCCCCACTTGTCTT
TCTACCTCTCCTTCCTTCTCTCTCTGTGCCTCTTCTTCTCCCCGTCCCTCCACGTCCCCCGTGTTACCTG
TGTGATTCAGCTGCAGCTCAGCTACGTCCCACTGGAAGAAAAGACCAGAAAACAGGGCACGGACGTAAGC
AGAGAGGTTCTATGTGTCCCCTGTGCCAGGAGCTCTCTTTTCTCTGTCCATCCTCCTTTCCACCTCTCAT
CCCACGGCGCGCACTGGTGATTTTATTTCATTTTATTTTTTTTTACTCTTAAGACTTCTTAAAGAGGAAG
CAGGTCTGAACCAGCGGGGAACGTGAAGTCGAGACCTTCAAAAGAACTTCTTCGTTTCCCGAATCGTTTT
CCTGGATGTCACTGGGCGCGAGAGCACCGCGCGCAGCGGACTCTCTACGCTGCAAAGGCGCCCGGCGCGG
TCCCCAAAGGGACCCCTCCCCCACCCGGAGCGAGTGGCTGGGGCCATCCCGTCCTAGGAGGCCCCAGGAA
GCCCCCACGCCCAGCCTTGTGAAGCCCCCAGCAGCCTCTGAGTCAGCCTGCCGGGGGAACACCAGAGCCG
AGTCATCTTCCATTTCCATGTGTGCCTACCTTTCCCATCCCGGGATCTCCCTGTACCTCCTGGCAGGGCT
GCCTGTCTCGTAGCCCCTACACCGGGCAGCCTGGTCCCCGCCTCGCCTCCCTGTTCTGGTCTCTGTCTCT
GCCCTACCAGATCTTCCTCTCTGGCCTCTTTGTACCCATCTCATTCTGTCTTTTCGGTCACGCGCCTCTC
TATGTTTTCTTCCCAACTTTTCCATCTCAACTCACCCCAAGTGACTCGAGCGGCAGCTGCTGCTGAGCCT
GTGCGAAGCGCTGGATGCACACCACGAGGCAGATCACCAAGCCCGCGACCAATGGGACCAAAGCAGCCCG
CAGGACGCACCCATAGGGCCTGCGCCGCACCGAGCAGCCCGAACCCTCCTCCGGCATCGCCGCGGCGATC
ACCTCCGCTAGCGCAGGAGGGGCGATGGGGGCGCGGAGCGCTGCCGAGAAGGAAGGAAGGAAACTGCAGC
CCCCTCCCGGGGCTCCTGGGCGTCTACTTGCTTCAACCTGTCAGGGGACCAGCCTGCCCCTCTCTG

Protein Sequence : Show Sequence

>gi|4507605|ref|NP_001243.1| CD70 antigen [Homo sapiens]
MPEEGSGCSVRRRPYGCVLRAALVPLVAGLVICLVVCIQRFAQAQQQLPLESLGWDVAELQLNHTGPQQD
PRLYWQGGPALGRSFLHGPELDKGQLRIHRDGIYMVHIQVTLAICSSTTASRHHPTTLAVGICSPASRSI
SLLRLSFHQGCTIASQRLTPLARGDTLCTNLTGTLLPSRNTDETFFGVQWVRP

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

4. Cxcl10

Gene Name : Cxcl10
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 15945
NCBI Protein GI : 10946576
Genbank Accession : AC109603
Protein Accession : NP_067249
Taxonomy ID : 10090
Chromosome No : 5
Gene Starting Position : 92346637
Gene Ending Position : 92348888
Gene Strand (Orientation) : -
Protein Name : chemokine (C-X-C motif) ligand 10
Protein pI : 10.57
Protein Weight : 10391.5
Protein Length : 98
Protein Note : Also known as C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10

DNA Sequence : Show Sequence

>gi|372099105:92346637-92348888 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
ATTTCATGTCAGTTATTATTACTTTATTACATTTTGGGTCTCTTACTACTTTCAATACAGTACATTGTCT
TTTGAATGTTACATAATACAAAAGGTATTGGACGGTTTTAAAAATAAACTTTAACTACCCATTGATACAT
ACTTGATGACACAAGTTCTTCCATATACAATGCAAAGCATACAAAAAATACATTAGGAATTCTACTTTGT
ACAGTCGTTCATTAAATAGTATTTACACATACATTTTCAGGTTCCTCTGAGTATCTTGATAACCCCTTGG
GAAGATGGTGGTTAAGTTCGTCCTTACAAACTTAAATTTGTAGTCTTACATCTGAAATAAAAGAGCTCAG
GTAAACTTAGAACTGACGAGCCTGAGCTAGGGAGGACAAGGAGGGTGTGGGGAGCAGCCTGGGCATGGCA
CATGGTGAAGGGCGTCGCACCTCCACATAGCTTACAGTACAGAGCTAGGACAGCCATCCCAGCCACTTGA
GCGAGGACTCAGACCAGCCCTTAAAGAATTCTTGCTTCGGCAGTTACTTTTGTCTCAGGACCATGGCTTG
ACCATCATCCTGCAGGAGGAGTAGCAGCTGATGTGACCACGGCTGGTCACCTTTCAGAAGACCAAGGGCA
ATTAGGACTAGCCATCCACTGGGTAAAGGGGAGTGATGGAGAGAGGCTCTCTGCTGTCCATCCATCGCAG
CACCGGGGTGTGTGCGTGGCTTCACTCCAGTTAAGGAGCCCTTTTAGACCTGTAGGAAGAGGAAACTGTA
TTGAGCGGAATAGACTCTGCTTTCACTTTGGGAACCCAAAGACCCTGGGGCAAAAGGCCTGGTGCATTTT
CCTCTGCTACACAATCTCCATGGGTCACGGGTGGGTGGGGGTTGAGGGGACAGAATGGGTTTGTGTGTTT
TTTGTTTTTTTTTTTTTTAAGTGAGGAAGTGGGGGGGGGGTGGGCATTTGGCAGCTTTACCCGTGACAGG
TATAATAACTGCTCCACTTCTAGACACTAGGAATGTATGACCTAAGAAGGCACCTCAGTGTCTGTGTAAA
TGGAGAGACAAAGGCACATGCCGGTGCCATGAGTTTAGGGCAGATGCCCCACAGCAAAGCCCAGGGCAGG
CGCTGCTCCCCAGACCACCATTTCCTGAAAAAGCAACATCAAACCTACCTTTTTTGGCTAAACGCTTTCA
TTAAATTCTTGATGGTCTTAGATTCCGGATTCAGACATCTCTGCTCATCATTCTTTTTCATCGTGGCACT
ATAGAAATAAGAGTGAGATACTCAGATTAACGTCAGGCCAACAGAGCTGATGGCCCTGCAGGTCCATGTC
AGAGTTCAGGCACTGTGCCTTAGTTAGAGTCACAGACCCGTCCCTATCTTTCCTGGTTTCCCTCACTTAT
TTACTGTGTGGTTACGGGAGGGACGTTTATCGGCAGGTGGGCTTGTACTCACATGATCTCAACACGTGGG
CAGGATAGGCTCGCAGGGATGATTTCAAGCTTCCCTATGGCCCTCATTCTCACTGGCCCGTCATCGATAT
GGATGCAGTTGCAGCGGACCGTCCTTGCGAGAGGGATCCCTGCAGGAAGAGAGGGGGAGAGCCAGGAAGG
TCAGCACACGGTTTCATTTTAGGCACTTAAAATGGATGAGGTAGTGTTTAGCAGACCCTGTCTGATCTGT
GGAGAGACGCATCCCATCGTATCATAGGGCTGAGTGTTTGAATATCTTGGTCATGGTAAAGAGAGATCTG
CAACTTGTCAGTTACGAAATCCTGCATTTATGCACTGCTCACAGTATTGGTTAGGCTCAGGCAAGGAGGC
ATCCGTGCCTTACCCCATCCCACTCTTTCGCCCTTTCTCCTGACTCTCTCTTCCCTCCTTCTTCCTCCTC
CCTCCTCCTTTTCTCCCTTTCTTCCCTTCTTCGTTCCTTTGCTTTTATTCTATTTTTCTCTGACTTCAAC
CAGCCTATGATAAACTCTGCAAGCTGAAGGGATTTCTTCCTGCTTCCCTCTTCTTTATAGGTACACACAT
TAAAGCTTAAATACATGCTGTGTTTAGAACTGAGCTTAATCGTCTGACTTCTGACTTCGTTAATTAAATG
GCCTTGGTGTCCCTTACCTTGAGTCCCACTCAGACCCAGCAGGATGAGGCAGAAAATGACGGCAGCACTT
GGGTTCATGGTGCTGATGGGGAGGCTTCCGGAAGGTTGGCTCGGGATGTCTCTCAGCGGTGGATGAAGCG
CTTCTCGAGTGC

Protein Sequence : Show Sequence

>gi|10946576|ref|NP_067249.1| C-X-C motif chemokine 10 precursor [Mus musculus]
MNPSAAVIFCLILLGLSGTQGIPLARTVRCNCIHIDDGPVRMRAIGKLEIIPASLSCPRVEIIATMKKND
EQRCLNPESKTIKNLMKAFSQKRSKRAP

Molecule Role : Vaximmutor
Related Vaccine(s): MVA/IL-15/5Mtb vaccine

5. Cxcl11

Gene Name : Cxcl11
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 56066
NCBI Protein GI : 9507071
Genbank Accession : AC122365
Protein Accession : NP_062367
Taxonomy ID : 10090
Chromosome No : 5
Gene Starting Position : 92359543
Gene Ending Position : 92363295
Gene Strand (Orientation) : -
Protein Name : transcript variant 2
Protein pI : 11.02
Protein Weight : 10670.03
Protein Length : 100
Protein Note : Also known as Ip9; H174; Itac; b-R1; Cxc11; I-tac; Scyb11; Scyb9b; betaR1

DNA Sequence : Show Sequence

>gi|372099105:92359543-92363295 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
CTTTACTTTAAGATAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGAAACTCCTGGTCTGTAGACCAGGTT
GGCCTTGAACTCAAGAGATCCTCCTGCCTCTGCCTTTCAAGTGCTGAAATTAAGGTGTATACCACCACAC
CTAGGTAATTTTTTTTATTGAAATTTTTAACAGTACCTTTTTATCATATTTTTTTCTCATCTTCAGCTCC
TCCTCACTAAAGTATATTCCTTTCTATTTTAAAATTTTGTGTAGCCCTGACTGTTCTGAAAGACCAGTCT
GGCCTGGACCTCACAGAGATCCGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGTATGTTTCT
AAAGAATTCTGACAAAAACTTGTGTAGCTCTAAAAAACAAAACAAAACAAAACAAAATCTAAAGGCATCT
CAATGTCCAGAGAGCATGTAGTTGTTGATGATTTAATAAGTGTTATAATAGCTTACTAGAGAAACACAAT
TCTTATTCTTTTCAAGGAGAGACAGAGCCAGCCATCCCTACCATTCATTCACAGTATGACCAAGGAAAAG
AACAAAGTACATATTTATATATTTTTCTCAAAGGCAGACTCACAGGAAACAGACTGAAAGAGGTGCACAG
ATTTTTTTTTCAACATTTTTCTTTGGTGAACAGAACCAATTTATTTTAAAAATCTATCCTTGTTATTGTC
ACTGATAAAACCAGACGTACAGGAAATACACATCTTAACCCCTTAGAAGGCCTCAGGGGGAAGCCAGTGT
GACTAGCTAGGAAACAAACAGTAGTGTTCTTACTAGTTTCTACAACGCAGAAATGAATCGTAGTCTTTTT
AAATGAACAAACAAGACAAAATGTGGAAATGTCTTCCTAGATGTTCGTGTGCCTCGTGATATTTGGGGAA
AGGAATGTTTATTTGCATGAACAATTCTTTGAGGAATGTTTTACTACACACAGAGATGCTTTTGAATCCA
GCACTGGTCCGGATTGCAGTAACAATGCATCCACTGTAACCACAGAAGGTAGCGTGGAGTGTGCACGTGA
CTTTTAGAGACAAATATTTTTCATAGCCAAGTAGAACAGTTTAGATGTATCGGACCTTCTAGAAAGTTCT
GCAGCCTGGTAATACGTGGCTGCATGTTCCAAGACAGCAGAGGGTCAGGTTCCTGGCACAGAGTTCTTAT
TGGAGGGCTCACAGTCAGACGTTCCCAGGATGTCACATGTTTTGACGCCTTAAAAAATTCTTTTTTTCTA
TTGCCTGTAATGAGAAAATGAAGAGTTTTACTTACTGGGTTGTTTCGTTTCCTCAGTAATTAAGAGCTGA
CCTGTAAAAACAACGCGGAGGGGTAAGGATGTGGCCAGGTTTAACCCAGGACCCCTTAACTGGGTGCTTC
TGTTAGGATGGTAATGGGGTCTTCCGGCAGTCGCCTGTTCTCTGTGTTCCAGCACTCTCGTGGGTAAAGC
ACTTTCCGACCTGGCCGACTGGCAACCTACCTGCATTATGAGGCGAGCTTGCTTGGATCTGGGGTCCAGG
CACCTTTGTCGTTTATGAGCCTTCATAGTAACACTGGTAAGGGAGACACAAAAGAGTCTTAAACTTTGGT
GGGAAAACATCAACCAAACAAAAGCACCCATACCATTGTCACCTCCAGCACATACATCACTTCAACTTTG
TCGCAGCCGTTACTCGGGTAAATTACAGAAGCTTTCTCGATCTCTGCCATTTTGACGGCTTTCATCCCGG
GGCCGATGCAAAGACAGCGCCCCTGTTTGAACATAAGGAAGCCTGGGACAGACCACAGTGTTGGTTAGTC
AGGGCTGTGAAGTGGATGGCAGACGCATTTGTTAATCTCAACTGTGCAGACTGAAATTAAGTTTTTGAAT
CTCTCTGCATTAGGCTAATTTGCTGTATCCTTGATGATTTCCTTCCTTAATTTTTGTTAATTATTTAATT
TAGGTTGCAGACAGGACTCACTTGCTATTTGGACCAGGTTTGCCTCCAACTCACCACAAAGCCCAGGATG
ACCTTGAACTTGAAGCACTCACTACAAAGCAGCCCCCTCCCCCCATCTTGGCATTATCCTACCTCACTCA
GCTCACCCTCCTAAGTGCTGAAGTTATATTACAGTATGAGTATGCTATGAGACCGGCTCTTGATTACTTT
AAATAACACCTACACAGTATACAGCAACCCAGAGAACAAAGTGGCTATTTATTTGGAATTATTTTATGAC
TGGTGGAATGGGTATACAAGCGTCTTTCACAGTCGCTGGTGATCTGTTCTTTACTAAATGAGCAAATAGT
TGATAATTTCTTTGATCTTTGTCTTCCCAGTTCAAATTCTAGATTAAGACTAAGTATTATGAATCATGTT
GTCTCCCACAGCTGAAACTGTCTTTAAAGCTTCCAGATGCTCCCAGGGAAACCTCAAAGGTTCATTATTT
CATAAGAGCAGCAAGAGTTGTAATAATGGCTTGGCTTGTCACATTAACTTCATATCTGGCTTAGCCCAGG
TCTGCTGTGATAATCGTGTAAGAACCATCAGCTCACAGGAGAAACACTTCCAGGTTCAGTCATGGGACAT
GTGAATAATGCTTCTATTTAGAAACAATATTCTTTGGGAAGAGCTTGTGGAGCATTGTCAGTATCCACAC
TACCTTTAAGTCACTCTTGCCCTCAAGTGACATATTGATCAAGCACGTATGACATTTTTATGACCATTGC
CCCAGAGAGAAATTTCCTTTTCTCTATTCTGAAAGTATTGGTCATAACAAATTAGCAGTCTCCTGGTTAA
AACATTAATTTTTTCAGAATACCAGCATAATCCCACATAAGATTCTCTGAACAGGCATTGAGGACTGGAT
AATTGTCAGAAAATGAAGAAATTGGGCAGGGGTGGCGCACGCCTTTAATCCCAGTACTAACAACTGTGGA
GGAATACAATGGCATTGTTTACTGCCTAATATTTGTAAGTTGCTCTGACCATAATATCTGTGGTTTATTT
GAAATACAAATCGCCAGGTGTGGTGGTGCACGCCTTTTATCCCAGCACTTGGGAGGCAGAGGCAGGCGGA
TTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCC
TGTCTTGAAAAAAACCAAGAAAGAAAGAAAGAGAGAAAGAGAGAAAGAGAGAAAGAGAGGAAGAGAGGAA
GAGAGGAAGAGAGAGAGAAAGAGAGAAAGAAAGAGAGAGAGAGAGAAAGAAAGAAAGAAAGAAAGAAAGA
AAGAAAGAAAGAAAGAAAGGAAGGAAGGAAGGAAGGAAGAAGGAAAGAAAGAAAATGAATCCATAAACTG
ATCATTTTCTTTAAGGATTTCCAACATTTGCTGCGCCATTGCTAGAACTTCTTTTTTTAATTATTTATTT
TTTATTAGATATTTTCTTTATATACATTTCAAATGCTATCCCGAAAGTTCCCTATACCCTCCCCTTAGAA
CTTCTAATATGTGAAATAAAGTAAATTTTTAAAACTGTATATTTTAGAGAAAAAGATATTTGAAAAGTAA
CACAAGGAACAGACAGACTCAGAAGCTACGGGCACCAAATACCTTGAGCAGCTGTGGCCCAGATATTGCA
GCCAGGGCTATGGCTGTGACCTTCCTGTTCATCTCAGCAGCCGCTGCAGCTCTCTGCCTGGGCTTTGGAG
ATCTCTCTTTGCAGAGAGCAACGGCAGGCAGGAAGGCTGAACC

Protein Sequence : Show Sequence

>gi|9507071|ref|NP_062367.1| C-X-C motif chemokine 11 precursor [Mus musculus]
MNRKVTAIALAAIIWATAAQGFLMFKQGRCLCIGPGMKAVKMAEIEKASVIYPSNGCDKVEVIVTMKAHK
RQRCLDPRSKQARLIMQAIEKKNFLRRQNM

Molecule Role : Vaximmutor
Related Vaccine(s): MVA/IL-15/5Mtb vaccine

6. Cxcl9

Gene Name : Cxcl9
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 17329
NCBI Protein GI : 110626107
Genbank Accession : AC109603
Protein Accession : NP_032625
Taxonomy ID : 10090
Chromosome No : 5
Gene Starting Position : 92321330
Gene Ending Position : 92328078
Gene Strand (Orientation) : -
Protein Name : chemokine (C-X-C motif) ligand 9
Protein pI : 11.28
Protein Weight : 14046.52
Protein Length : 126
Protein Note : Also known as CMK; Mig; MuMIG; Scyb9; crg-10; BB139920

DNA Sequence : Show Sequence

>gi|372099105:92321330-92328078 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
AGACATTGAAAAACATGTTTATTTTGGAATTTAGTGCACTTTTACTGATATCACTACGCTTTCACGAAAT
AGCTTCAGAGCACATATGCTAATTTGCTTTGAATTAAAAATTAGAGTACACATATGAAGTCATACTGTAG
GCATATGCATCCAACATAACTATTTAGTAAAAATTACACAGGCTTTGGCTAGTCGTTATGCATACTACCC
AGCTGTCAGATGCAAGGGTAGCTAACTAGTTAGGAAACAATTTTAGACTTTTTTTTTTTTTTTCCAAAGT
AAATTACCGAAGGGAGGTGGACAACGTTTTGACTGTGCAATGTTGTTTGGATGGGAGTCCCACCAGCAGC
ACAAAAACCACCTGCATGCAGCGCCAGGAATGGCAGTATTTGAGAAAAAGCATGCTCAAGACGGAACTTC
TGCCCAGAGACATTCTTTTCTATCTAATTCATCAGACCTCCTCTCTAGAGCAGTTTCCAGCTCCCACACA
GGCTAACTTGGCCCTGTCCACACTTCCACTCGCAGAATTTACTGCTTCCTGTAGCTCTTAATTCCTTTAA
AAATATCTCTGTAAAGATTTTTATTTGCCGTCTGAGATTATGGGTGTATGTCATGGGGGCCTGTGTACAC
CTGTGCAATGCTTGCAGAGGCCAGAAGAGAGAAATGGGTTCCCTGGAGCTGGAGTCACAGGTGGCCACAA
GGCTCACGCACACGTGGGGGTGGGTGCTGGGAATGGAACTCTGGCTCCCTCTCCAGCCCTGATCTTTCCA
TTTCACCACTCCTAGCCTGATGTGGCAATCCATACAATCATACACTGGGATTTCTGCAATGAGGGAGGGA
AGGTGGCAAGGAAATTCATATTCACCTAAGATAAACTTGCTAAAGGATTTGGCAGCTCTTTTGTTGACTC
CTAGGGAGCTTGTCCTGATCAAGAATGATATTAGAGGAATTTTGTTTTGAGTGAGGAAAGATCTAGTATT
TGCCAATCAGTCAAACCTGTCTTCCATGTAAGCTTATGTTTCTAGATGGCCTGTGTAATTTTTCCTGAGC
AGTCCCAAATATCCGCAAATGTCTTGAACATGTGATTCCTGTTTGTTAAAGACACGAGCCTCCTGTCACA
AGGTAAGGCTCTTTTTCACCCTGTCTGGCTCTGTGCGCTGAAGATGTCTGAAGATAATAAGAAAGCTAGA
TAATTTAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGGAGGGAGG
GAGAAAGGAAGGAAGGCAGGCAATCAGGTGTAGTAGCACACACCTTTAACCCCAGCATTCAGGAGGCAGT
AGAATACAGATATGTATGAATTTGAGACCAGCCTTGTCTACTTTGAGAGTTTTAGGACAGCCAGATATAA
AACTTTCTTTAAAAAAAAAAAAAAAAAGTGGTAAGCAAGCAAGGAGGCCAAAGGTTAGTTAGGCAATTTG
GAGCAGCCGGGGATTGGCCAATGCCTGGTGTGTAACAGCCTTTCTACCTCTCACACATACAATTAGGAAA
TTATTCAAAACCAAGTTTTTGTAGGCACTGTGGAAGATTTAGGGGAATAGTCTGTGGTCCCTACTCTTAA
AAACCCGTCTCGTTGGAAAAAGTAAAACAAACAAACAAACAAATCCAATTAGATAGCAGCGAAGTGTTTA
CTTGTGAAACAGGAATAAATTTAAGAGAATTTGAGTAGCCAGAAAATGTTCTTTAAATTGGTTAAGTTTT
TGCACTGTTAGTGAAGGACCTGGTCCCTGCAACACCAAGTGTTCTGCCACTAAGCTACAGCCACAACCTT
CTAGGAGAGTTTTATTGAAGAGGCAAACTTAGAATAAGCTCTGAAGATGGGATCAAGTTAATAATGAAAT
CCCATGGTCTCGAAAGCTACGTGGGAGGTTCTGAAGGCCAACTCTGGTGGTCTCCACTGTAGAACCCCAA
AACCATTAGCACCATCTCTGAGACACAATGTGTGCAGGGGCTCTAGGCTGACCCAAATGCAGCTGCCGAG
GGCCTCTCCAGCTTGGTGAGGTCTATCTAGCTCACCAGCAAACAGACAGGCCTGGGATTTGGTGACGTGA
GCCTCAGAAGTCTTCGGAGGCTGCCAAATGTGTGGCTCTGGAAGCCAGACAGCTGTTGTGCATTGGATAG
CTATGAAGGAAAGGGACACTTGCCCAGATCTGATGTTATCACGACTAGGCTCAAGGGCGTGATGAAATTT
TGTTGGCTGTGTAGAACACAGAGTTTTTGCCTCTAGCAATTGTTACTATTGGAGTTTTGCAATTAACTTC
CTAAAGTTAGTTATAATTTCAAATTAACACTTTATGTTTTGTAGTTGAGTTAATGTATTAAAAGCCTATG
TATTAAAGGCTGCTCTGCCAGGGAAGGCTTTTCAGTACAATCTAAAACCCATTAAGATTCAGGGTGCTTG
TTGGTAAAGTAATGGTCTCTTATGTAGTCTTCCTTGAACGACGACGACTTTGGGGTGTTTTGGGTTTTCT
GTTTTTCATGTTCTTTTGATGTTTTTTCCCCCTCTTTTGCTTTTTCTTTTGGCTGATCTGCAAGAAATGA
GAAAAAGGCAATGCTTTCCAATGTTGTAGTTTCTCCCCTTCGAGATGGTCCAACTCCTGAGTGTGATCTC
TCCTTATCCTCAGAGCCAGCTCACGTCGCTGCTCTCTCAGAGGCGCTGGCCTCATTATCTGCTGCCCCTA
CATTAAAGAAGCTACAGTTCCCTCTTTGCTTGTTTGTCTGTTTTCTGGCATCTTGCTGTCTTTCATCTTC
TCCCTTCATCTCCCACCTGGGCCAAACACAGCCCTTTGGGGCTGGCTGGTAGCGTTATCACTCACTTTGC
ATATTAGCCAGTGGTTCTCAACTTGTATGTCGAAACCCATTCGGGGGCACATAGCAGATATCCTGTGTCT
CAAGTATTTACATTATGATTGTTAACAGTAGCAAACATTACAGTTATGAAGTAGCAGTAAGATAACTTTA
TGGTTGGGGGTCACCTCAATATGAGGGACTCATGCATTAGGGTCTCAGCATTAGGAAGGTTGAGAACCAC
TGAAACTGGACAAGGCTAGGCGAGTGAGTAGCCTGAAGCTTAAGGGCTAGGGTCTAGGGCAGACCCAGCA
GTTAGGTGTGGGGACCCAAGCTCCCCACCACATACCCCATGGCTCTTCTCAAACCCAGCTCCTTAGCTAT
CTCACAGCTCTTTTCCCTTTTTCCTTGGCTCCTTTCTCATCTCAATCCCTTTGTTCTCTGCATTTAAATT
CCAGTTACATTACTTCAGACTCACTGAACAGACTTTACTCAAAACCACCAACGCCCACACTGTACTTAAT
TAACTGCCTGGTAAATGTCTTATCCTCAAAGATCCAGATCAGAGATCACTTGATAGGAGAAGTCTACCTT
AATACTTTCTATATTGTCTCTTGGTCAAGTCAATTACCCTTTCCATCATAATTCTCAAAGTTCTCATGTA
CACACTATTTATTCTAAATGTCTCTCACCACGTCTGTTGATTTTTATACATTGTTAATGACTATCTGCTA
TATGGGGGAGGGGTGATAGCTTATTAATTTTGAATCCTTAGTGTCTTACTAGTTGATGCTATTCAAAAGG
ATGGAAAATGGGTCCTTAATGGGTGTGCCCAGGATCTGCTGACTTTGTGAGCTGACCTCTGAAAGAAAGC
CATTTGGGTTCCTCCCCCACCAACCTTCTTTTCCCATTCTTTCATCAGCTTCTTCACATTTGCCGAGTCC
GGATCTAGGCAGGTTTGATCTCCGTTCTTCAGTGTAGCACTGTGCCAAAGAAACACCAGTGAGCTTTCTC
CCCCCAGTAACAACTTCATAATTCTTAGTAGAAACAGTGGAGGAAAACGTGCATTTTATTGAGTTTTCCA
GTGAAACCACTTAAAATGTGGCAAAACGTCTTCCTAAGATTAACAGGTATATTTTTAAAAACTGTAGCAG
ATTTTTCAGGAGAAACCTCAATTTCTAAAAATTAATTTCTCTGTATAGCTTGAGAATAATAATGGCTATT
CTATATAGAATTCCTTATTTTAGTTGTCAAAGGTCAAATATATTACATACATGTATGTATATATATATTT
ATATCCTTTATATATGTATGTGTATATATTTATATCCTTTATATCGTATACATACATACACACATATAAA
TATATTATATAATATTATATATCCTTTAATTTCATAGTTCTGATTTCTAAGAGGCTTGGAATGGTGGTTT
ATTCCTGGAATCCCAGCATTCACGTGGACTGAAGTCAGCCTTGGCTACCTAGCAAGACTCTGTCTCAAAA
CAAAACAAACACGAAATTCTAGTCTCTAGAAAAGAATTACTAGGAAAGAATTAAACACAATCCTACAACA
GTAATAGTCTGACAACAGTGAATTTTTTTTAAACATAACCGTTGTTTCTAGCAATGGGCCATGTGCTATA
CAAATTATGGCTTATCAATATGATGTAAAACAGCTTAGCCATCAAAAGTCATTATTAGGCCAGGGAGTTG
ATGTGACTGCTAAGCATACTTGCCTCATAAGCCCAGAGAGCTGAGTCAACCCCAGAACCCCAAATTAGGA
CAGAAGAGAAAACCGACTCCACCAAGTTGTTCTGATTGCTGTATGCATGCTGTGGGATGTGTTCTTGAAC
ATACAAAGTATGGGTTAAAATTTATTTTATTTTTAACTTCTTTGTGTCTCTGCATGTGTATGTGTACTAG
TGTGCAGCTGACCACAGAATCCAGAAGAAGGGATAGGATCTCTGGGGTGGAGTTACAGATGGTTGTGAGT
TGTACACTGTGGGTAATAGAAACCAAACTCGGGTCCTCTTAACCTCTCAGCCATCTCTCTGCAACCCCTC
AAAAAAATTTTAAATGCCGCTTTTAGGATTCAGGGTTAGGGTTAAGTAGAGAGAGAGTACATGCATAGCC
TGTGCAAAATCCTAGGTTCAACCCTAGCACCCCCAGACAACTGACAAGTATGGCATTTTTAGAGAAAATT
AATTGTCATGAGAAACTGTCGTTGTTATTCTGTTAAGGAAAAAACAAATTACAAATGGCAACTTCTCCTC
CCCTGTAGGGAAGATGTCCAGGTTCCTACACTCTCAGATGGTACCTACGTACCTATATGCCTTACTTAAG
ATAAAGTCTAATGTGTAAACTAAACAGACTAAGGTATTAACCGCACATTTTATTATTTAAAGATATGTCA
TCTTCCATTTTCAGTTTCCAGAAGAAATTTCTTATTTTGAAGAGGAAGAAAGAAAAGGAGTAGACAGATT
TATGGGGGGGCCTCCTGCCTACATGAACAAGTAAGCAAGCTGAGGGTGAGAATCCCACTAAGTCCAGTGT
AGGATGCATGCGTTGGGAGCATTTACTTACATGATTTCAGTTTTGTTGCAATTGGGGCTTGGGGCAAACT
GTTTGAGGTCTTTGAGGGATTTGTAGTGGATCGTGCCTCGGCTGGTGCTGATGCAGGAGCATCGTGCATT
CCTTATCACTAGGGTTCCTAGGCGGAAGATAATGCACATTACTTAGAATGACATCCTTTCGAATGAGGCT
TGTCTCAGTCACTATTGCTGCGACTGAGTGTCTGTGACTGCTTTTCAAGGTAGGTGGTATTTCCATTCTG
TATACCAGGAAGCCAAGGTTAAGGGCAAAGTCATTTAGCAAAAGGGAGCAGGGGAAGAACTTCATTTTAG
GTCATTTAGAGTTCTTAACAACATCACTTCACAACCTTGGAAGAAGAGAAAACCTCCATCATACTTAAAT
CCCTACTGTTAGACACACATATATGCAACCTGTCAGTACTACGGGGACCGGTTTCTTAAGCAGTTCCTCA
ATCTACTTCTTGCTACTGGGGTCATGGAGTTGATGTAGGTGTGGACAGAAAGCTCTACTTACAACAATTG
ATTACCATTTCAATCCTATTCCCTCTCATCCTCACTGCTCTGAAAAGTCTCTTTAAACCTATGTCTCCAT
CCCTTATTGCTTACCCAGGTCAGTGTCTATTTACACAGGTTTTGCCAACACACAGTAACTATGGCACACC
TACTTTACACACGTGGAAAACGCTCTCTAGGAAGGTTTTTTTTTTTTGTCCGAAGTAAGCAAAGTCATGA
ATAAGGAGGTCAAAGGCAATCTCTCTGACTTCTGAATTCTCAGTTGGTTAACCACAGACATTCTTATCAG
TGCACAGCATCGGTTGAGGAAAGTGATGCAGGAGACAACTTAGAAAAGGATCTCTTATGTCACTCTCTGT
TGCCAGGGCTGCGGATGAAATATTTCACAGGATAAATTCGAAGTAAAGCACCCAGAATCCCCTTTACATC
TGAGAAATGTTTTTTCATTATTAAAACATTTAAATATGCTTTGAACCCTCATTGGGAACTCACAATACCT
ACGCCAAGAAGGCATATTTAACTTGGCAGGTATAGATTACGACCTTTGGATAACTGCTCACAGCATCTAA
CTGCTTTGATGACCAAACAGTATTACTTTTTATTGCATGCAAGACATTCAGCTTTATCTGCTGGCCTGAG
AAGGCTTTTACGGTGATTCAGTCCTCAGAACTTACTAACGTAAAACTCCTCACCTCGAACTCCACACTGC
TCCAGGAAGATGATGCCCAAGAGGAAAAGAACAGCGGACTTCATGGCAGAGCTGAGTTCTATTGAGTCAC
TGTGTTGGAGTGAAGTCCGAGAATGTCTT

Protein Sequence : Show Sequence

>gi|110626107|ref|NP_032625.2| C-X-C motif chemokine 9 precursor [Mus musculus]
MKSAVLFLLGIIFLEQCGVRGTLVIRNARCSCISTSRGTIHYKSLKDLKQFAPSPNCNKTEIIATLKNGD
QTCLDPDSANVKKLMKEWEKKISQKKKQKRGKKHQKNMKNRKPKTPQSRRRSRKTT

Molecule Role : Vaximmutor
Related Vaccine(s): MVA/IL-15/5Mtb vaccine

7. Defb3

Gene Name : Defb3
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 27358
NCBI Protein GI : 7304997
Genbank Accession : AC113099
Protein Accession : NP_038784
Taxonomy ID : 10090
Chromosome No : 8
Gene Starting Position : 19293360
Gene Ending Position : 19295338
Gene Strand (Orientation) : +
Protein Name : defensin beta 3
Protein pI : 11.07
Protein Weight : 6827.63
Protein Length : 63
Protein Note : Also known as BD-3; MGC129397

DNA Sequence : Show Sequence

>gi|372099102:19293360-19295338 Mus musculus strain C57BL/6J chromosome 8, GRCm38 C57BL/6J
CCAGGCTTCAGTCATGAGGATCCATTACCTTCTGTTTGCATTTCTCCTGGTGCTGCTGTCTCCACCTGCA
GGTGAGTCAGGGGAGTAGGGTCATCCCACACTGATAGGAGAGACAACACCCTACATCAGGTGTGTGTGTA
TGTGTGTGTGTCTGTGTGCCTATGTATCTCTGTGTTTGTCTCGGAGTCTCCCTCTCTATGTATCTTTCTG
TTTCTCTGTGTTTGCATCTCTCTCTGTGTCTTTGTGTTTCTGTGTCTCTATGTTTGTGTCTGTCTGTCTG
TCTATCTCTCTCTCTTTCTGTGTATGTGTGCATGTGTATACCTTTATGTCCCTTAATCTTCGTGTGTTGG
TTTGTCTCCCTCTCATTTTATGTGTGTGTGTTCCTCTGCGCATGCATCTGTCTTTCTGTCTCTATGTGTA
TATGTCTGTCTTTCTGTGTATAAGTCCATATCTATGTTTCTGTCTGTGTCTTTTTCTCCTTTTTACATAT
ACCCTCCTTACTTTTGCATTTTTCTTTCTTTCTTCTAAGCATTTTAGAAAATGTGTATTGTTGACTACTA
ACACAAACCAAGGACTCTTTCTTTTTCCCTTGAAGGAGTCTAAGGTAGACACATGCTCTGCTCATCTGCA
GCAATTGACTTCCATTTCCTAACTCCTGATGCTAAGCTTAAGATTATCAAATGAGTGTCTTATAGGTAGC
TTAAAAATAGTAACATGAAGAATGTTTGATTCTTACATGAACAGAAAGGTATTAATTTAGAAATCTTTAC
ATATAAGGAGTAGAGTCTCTGCTTACTCTAGAGAACTCCTCAGAATAACCAGCCTAGCCTGTTTGAGAAC
TTCTGCTCCCCTTAGCCCCATGGAATACCCCTGAGCACACAGTGTCTACAGGGTTAATGAGGTTCATAGC
ACAGGGGAGGCTATTAAATCCTGTCTCCCAAACTTTTGCTTAAGTGTATTTAGTGGAAAAAATTTATTTT
CTGTAAATTATGTGTATATGTATTGATTTGTATATGGGTATGTGCCAAGTAGTACAGTACTCACAGAGGT
CATAAAAGGAAATCATATCTCTCAGAGATGGAGTTATTTATAGGCACATATGGCCTGCCTGATATGGTAC
TAGGAACTTATCTCAGGTCTTCAGCAGGAGCAGTATGCACTCCTACAGGCTGAGCCATCTTTCCAGGCTC
CAAGCTGTGTTTCATACTTGACTTTAGACAAACAGAATAATGGGGTTAGACAACTCATGTGAGGGAGCTA
AAACAAGTAATGAGTTGGAGAGGAATTGATGAACACATCTTAGGCCAACCTGTGGCCTCTATATGTGTGT
GCACACATGCACATTCATGTGCACAGTCACACTCAGTGTCTGTTTCACATGCTAGAAGTAGGGGCTTGTG
TGTGGAGAAGCAAAGGCAGGGCTGCTAAGAAGTAGCTAGGGAGTCACACAAAATCTTTCCTGAGCTACTG
CATGCTGGCTTTGAATACTCCAATCAGTCAGGGGTTGAGCAGATCCTTCAACATTTGGGTCCTAGGAATA
CCAGCACCTCCTCTACACTGCAACTGTGCCTGTCTCGAACAAGAATTGGATTCAGCCCTGCTAAAACAGA
CATTACTGTTTGGGCAGAACAATCTGCTGGGATCTACTTATCATAAGAACACATTGGTATGTATTTCTGA
CCTACCACTTTCAATTTTAATGTATGGTTTTTCTTACTTATCTTTCAGCTTTTAGCAAAAAAATCAACAA
TCCAGTAAGTTGTTTGAGGAAAGGAGGCAGATGCTGGAATCGGTGCATTGGCAACACTCGTCAGATTGGC
AGTTGTGGAGTTCCTTTCCTCAAATGCTGCAAGAGAAAATAGAGAAGACCAAGATCCCGTTAAACAAAGA
AAACACCAGAATTTGCTCCTCCATGAAGATGGACAAATCTTAAACCAAATTAATATTCCTTGATTAAATG
CAAACAAGTGCTCCCTAGC

Protein Sequence : Show Sequence

>gi|7304997|ref|NP_038784.1| beta-defensin 3 precursor [Mus musculus]
MRIHYLLFAFLLVLLSPPAAFSKKINNPVSCLRKGGRCWNRCIGNTRQIGSCGVPFLKCCKRK

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

8. Defb4

Gene Name : Defb4
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 56519
NCBI Protein GI : 9789929
Genbank Accession : AC116394
Protein Accession : NP_062702
Taxonomy ID : 10090
Chromosome No : 8
Gene Starting Position : 19198703
Gene Ending Position : 19201546
Gene Strand (Orientation) : +
Protein Name : defensin beta 4
Protein pI : 9.22
Protein Weight : 6929.73
Protein Length : 63
Protein Note : Also known as BD-4; 2310001F05Rik

DNA Sequence : Show Sequence

>gi|372099102:19198703-19201546 Mus musculus strain C57BL/6J chromosome 8, GRCm38 C57BL/6J
TGCATCTCTGCATCTCACCAGGCTTCAGTCATGAGGATCCATTACCTTCTCTTCACATTTCTCCTGGTGC
TGCTGTCTCCACTTGCAGGTGAGTCAGGGGAATAGGATGGTCCCACACTGATATGAGAGACAGCACCCTA
CCCGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGCTTTATGTGTCTCTGT
GTATGCCTCTTCGTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTT
CTTTCTTTCTTTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCCT
TCCTTCCTTCCTTCCTTCCTTCCCCCCTCTCTTTCTTTCAAGATTTATTTATTTATTTTATGTATGTGAG
TACATTCTTCAGACACACCAGAGGCACTAGATCTCATTACAGATGGTTGTAAGTCACCATGTGGTTGCTG
GGAATTGAACTCAGGACCTCTGGAAGAGCAGTCAAGTGCTCTTAACCACTGAGCCATCTCTCCAGTCTCC
TCTTTGTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTG
TGTGTGTGTGTCTGTCTGTCTTTGTCTCTGTCTGTCTCTCTCTAATCCACTTATTGCCCTCCTTTTTTTC
ATTCTTTCTTCTAAGCACATTTTAACAGTGTGTATTGTGTGATTGCTGAAACAAACCAAAAACTCTTTCT
TTTTAACTTGAAGAAGCCTAATGTAGACTCACTCTGAGCTGATCTGCAGTGCCTGACTTCCATTTTCTAA
TTCCTGGTGCTAAGCTTAAGATTATGAAATGAGTGTCTTATAGGTAGCTTAAAATTTATAACATGAAGAA
TGTTTGATTCTTATCTGAAAAAGTTATTCATTTAGAAATCTTTATATTTAATGAGTATAGAGTCTCTGCT
TGCTCTAGAGAATCCTCCAGAATAACCAGCCCAGCCTCTATTTGAGAACTTCCTTTCCTCCTACCTCTAT
GGAATACCCTTAAGCATACACATTCCACAGGGGTAATGGGGCTCATAGCTCAGAGGGGTTATAAATCACA
GCTTCATAAACTCTGCCTACATTTTATTTAGTGGAAAATGTTTATATCCTTTTAAATTATGTACACATAT
ATGGATATGTGCCAAGCAGCACAGTACCCACAGAGGTCATAAAAGGACATCTAATCTCTCAGAGATAGGG
TTATATACACATATGGGCTGTCTGATATGGTACTAGGAATTTACCCCAGGTCTTCTGCAGGAGCGGTATG
TACTCCTAACTGCTGAGGCATCTTTCTAGGCTTCAGATTGTATTTCATCCTTGACAAACAAAACAAAGGG
ATTCACAACTCAAGAGAGGGAGCTAAACAAGTAAGAGAGAACATGAAATGAATGAATGAATGAATGAAGC
AAGGACCTCAAACTGCATCAGACAGAAATGATATAGAGAGGAACATAATTTCACTAATTTTGTTGAAGGG
TTCTGGTTTGGAAAGGAGCATCTGTGTTCCATGAGATACACAGACACTTACTCTGCCTGACATTTAGATG
AATGCAAAGAACAAAGGAGGGAGAAGAGGGAAGGATGAGATTAAGACTGGATTTTAGACATTTGAAACTA
TCAAAAATAAGATTTTTTACTTTTGATTTCTATTGTAAATTACTAAGGAATCAAGTTATCATTAAGCAAG
TCTTATACATGGAGATTTGGGGCCAATACTTCATTTAAAATAGGTACCTGGAAGTTAAAATACCTTAATT
AAAAATAGGAAGATGGCTCAGTGATAAAAAGTGCATGTTGTGCAAGTATGAAGATCAGAATTTGGATCCC
AGTGTCCATGAAGAAGCATAGAGGGCTTCTGGTGTACATCTGTAATTCTAGCACAAAGAAGGATCCCACA
GGCACATTGGCCAGCCAGCCAGCCAGACCTCTCAATGAATTCCAGTTCACTGAGACACTCTAGATCAAAA
ATTGAGGCAGAGAACAATTGATGAACATATCTTATGACAACCTCTGGCCTCTATATGTGCCTGAAAACCT
GTTTGTACACATTCATACTCAACAGCTGTTTCTGATACAAAAAGAAGGATTTTTTTATGTGGAAAAGGAG
AGGCAGGGCTGCTGTGAAGAGGCCAAAGACTCACACAGAATCCCTACTGAGGTCTAGCCTGATGAACCTG
AAAATTCCCATCAGTCAGGGGTAGAGCAGACCCTTCAACATTTGGGTGTTAGGAAAACGAGCACTCCTTT
ATACAGCACCAGTGGCCAGAACTGGATTCAGTTCTGCTAAAACAGACAACCCAGTTTTGGGGGCATGGGG
ATCCTGCTTGGAATGTACTCAAGGTAATAGCACACTGGACTGTATTTCTGACCTGAGGCTCAACTCTAAC
ATGTGTTTTTCTTACTTATTCCAGCCTTTACCCAAATTATCAACAATCCAATAACATGCATGACCAATGG
AGCCATATGCTGGGGTCCGTGCCCTACCGCTTTTCGACAGATTGGCAATTGTGGCCATTTTAAAGTCAGA
TGCTGTAAGATAAGATAGATAAAAAGACGAAGAACCAGCAAGATGAATAAATTGCCAGAATTTGCTCCTC
CATGAAGATTCACAAATCAAACCCAATTAAAGTTCTTTGATTACCTTCTCTAACTCCAGTTCTAGGGGAT
GCACACCTTCTTTTGACCTTTATAACAACCATTTGCAGAGTACATACAGAAAAAATACCATACAAATAAA
ATTATAATAATAGTAATAATTATAATTTTAAAAATAAAGTCACT

Protein Sequence : Show Sequence

>gi|9789929|ref|NP_062702.1| beta-defensin 4 precursor [Mus musculus]
MRIHYLLFTFLLVLLSPLAAFTQIINNPITCMTNGAICWGPCPTAFRQIGNCGHFKVRCCKIR

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

9. Ebi3

Gene Name : Ebi3
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 50498
NCBI Protein GI : 7657052
Genbank Accession : AC154235
Protein Accession : NP_056581
Taxonomy ID : 10090
Chromosome No : 17
Gene Starting Position : 55952622
Gene Ending Position : 55957021
Gene Strand (Orientation) : +
Protein Name : Epstein-Barr virus induced gene 3
Protein pI : 10.35
Protein Weight : 23864.43
Protein Length : 228
Protein Note : Also known as EBI-3; IL-27; interleukin-27 subunit beta

DNA Sequence : Show Sequence

>gi|372099093:55952622-55957021 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
TGACTTCTCCCTTTTGGGGCCCTCCTTCTACCTCAAGGCCTTTCCCCACACATACACACAGCAGCTGGTG
ACAGAATCAGGGTTCCCGTCACCATCATCATCTCCTGCCCCATACACTGGACAACTGAGCCACACTGGGC
AGGTCCTTCCCTGGGGCCAGGTTCCCTGTGTGAGTCCCCTGTCCTTCACCCTCTCTCTGATGGGTCACTA
ACTCGGATCCAAGGAACAGAGCCACAGAGCATGTCCAAGCTGCTCTTCCTGTCACTTGCCCTCTGGGCCA
GCCGCTCCCCTGGTTACACTGAAACAGGTAGGTGACCACAGGGGGACTGGGGCACCTGGGCGGAGGGAAC
TAGTCCTTCCCTTTCAGTTTAAGGTCATATCTACCATCAGCTGGGAACTCACACCTATACCCGTTGGACC
TGTTCTCCTTCTACTACAGGGATGGGGAAACTGAGGCATGAAGCCTCAAAACCTCATTTATCCAGGCAGA
GGCACCCTTGAACTTAGACATTTGTGGAGCTAGACAGTTGACTCTCAGAAACCCGAAACCTGAAGCCTCA
CCTTTGTGACTCAGCAGCTGTAAAAGAAAAGAAAACGAAAAAGAAATGTAGACAATACTGGGTATAGTGG
GGCACACCTGCCATGAAGCTCTCAGGAGTTCAGGGTCATCCACAGTCACATAGGAGGAAGCCAAGTATCA
AGAGATTCCAATGTCCTGATCCACTCAGGGTAGGAGGCAGCCTAGGCCCCTTGGGAACCTGCTTCTAAAG
CAACAGAACAAGTGTGGGGTGTGGGTAGTCTAGGGAGTTCTTCAGAATCCACAGTTCAAGGCCAGCCTAG
GCTATGTGAGACCCTCCAGAAGGAGGAAGAGGAGGAGGAAGAAGGAAGAGGAGGGAGATGGAGGAGAAGG
AGAAAGGAGAGAAGGGAAATTAGAAAAAAAGAAGAAAAAAAAAAAAAAACCTCAGGCCAGGCAGTGGTGG
CACACGCCTTTAATCCCAGCACTTAGGAGGCAGAGGCAGGTGGATTTCTGAGTTCCAGGCCAGCCTGGTC
TACAGAGTGAATTCCAGGACAGTCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACCAAAAAAAAAAAA
AAACAAAAAAACAAAAAAACCTCAGTTTTCTCATCTAGAAATTGAGCTAAGAACCTTGGGGTTTGTAGTA
AGTATAGGAGCTCATGCTTCCATGCATAACAGGTCCTACATGGCCTACGGAAGCTCCTGTTCCTTTGTCT
CCTCCAGCTCTCGTGGCTCTAAGCCAGCCCAGAGTGCAATGCCATGCTTCTCGGTATCCCGTGGCCGTGG
ACTGCTCCTGGACTCCTCTCCAGGCTCCCAACTCCACCAGATCCACGTCCTTCATTGCCACTTACAGGTC
AGAAAATGGAAAGGGTTTAGGAGGAAGCCAAGTATCAAGAGATTCCAATGTCCTGATCCACTCAGGGTAG
GAGGCAGCCTAGGCCCCTTGGGAACCTGCTTCTAAAGCAACAGAACAAGTGTGGGGTGTGGGTATTCTAG
GGAGTTCTTCAGAATCCACAGTCCCCAAGTCCCCCTGAGCATATCACCCCCTGAGCGTGACCCACAGTCA
GCTGTGGGTGAGCTGGGGCCTCACGGGCACCCAGGTTCCTTTCGAGATTCACCATGGTGCTTCCCATGCA
GGCTCGGTGTGGCCACCCAGCAGCAGAGCCAGCCCTGCCTACAACGGAGCCCCCAGGCCTCCCGATGCAC
CATCCCCGACGTGCACCTGTTCTCCACGGTGCCCTACATGCTAAATGTCACTGCAGTGCACCCAGGCGGC
GCCAGCAGCAGCCTCCTAGCCTTTGTGGCTGAGCGAATCAGTGAGTGTCTGTCCTCCCTGCCCCTCTCCA
CCTGCTCCATCCCGTGGCTCCTCCCTGCCGCCGCCCCCCTACCCCCACCCACCTCTTGCTTTGTCCCTGC
AGCTTCCATCCTTTGGCTCTGCTCCCGCCTCACAGGCTTCCTGTCGATGCCCCGGGGCCTTTGCATTGCT
TCCTTCCTTGGCTTTCCTGGGTCTCTGCTCCAATGTTGGCTCCTCAGTGAGGCCTCCCCAAAAGTATATC
ATTAAACATAAGCAATAGTCACAGTTAAGAGAGGATGAAAGCCAAAGGTGGTGTACAAGTCTTTAATCCC
AGAGGTAGGCAGATCTCTGAGAATTCAAGGCCAGCCTGATCTACAGATCATGTTCAATGCAAGCCAGGGT
CACTCCTGAGCACCTGTCTCAGAAAAGAAAGCCAAGAACAAAAGCTGATGCAGGTCGGTGAAAGGCTACA
CAGCTCTCGAGGGTTTAAACCTGAAGTCTCGACATGATCGCCCAACTCCAATCCTAAGGATCTGCTCAAC
AGCAATAAAGCTGTTCCTCCCTACCAATCCTTGGCAACATCATTGCTGGCATCCAGAAGGCCAGTGCCCC
GTGCGTTCCCATGGAGCGATGGGCACCCAGTGCTTATCTAGCGTTACTCAGCCCTAACAAAGAGAGAGTG
GGGCTCTGGCACCAACAGCTGTGGCGGGACCTTGAGGACACTACGCTCAGTGAGCAACCCAGACTCAACC
CACCCAGTGTGGGCTGCAGTACAGAGGAGTCTAGAGACAGGAAGCCTGAGTGCTGAGGACAGAGAAGGGT
AGGAAGATGGCTTGTGTTGTCATGAAGGAGTGTTCCATAAGTAGGTGGAGTAATGAAAACGCTCTCTGCA
GATGCACGGGATGTCTTCTAGCAGAGACTTGTGCTCTCAGAATTGTATCTCAGAAAAGCCCTTCAGCCTA
CTGGAGACTTGCACTTTTCTTTGCCTGTGCAACCCTTGTGACTTCTATCAGCAGTGATGAGAGCGGTCCA
GCAAATGGTTTTTCCTTGAGCAGATAGACGAATGAAGGAATAAATGAATGCCTGCATGACAGATATCAAG
TGGATGAGTGAATGATTTAGTAATGAATGAATGCATAATGAGTGAATGAAGGCAGAAATGACATGAGTGC
ATGCACACGAATGACTTAATGAATGAACGAATGAATGCATGAATGACTTAGTGAATGAGTGAATAAATGC
ACAACTGACATAATGAATGCATAATGAATACATGAAGGCCATGAGTAAATGAATGAATGACATGAGTGAG
TGCATGAACGAATGAATGACTCAATTAGGGAGTGAATGAATGAATTCACAAAGGATACAGTGAATGACCT
AATGACTGGGAAATGGATCCCTGAACGAATGAGTGTATAAACGATAAGCGCAAATGAATGAGTTAATGAA
TCCAGAAACAAATCTGCACTTGAATCAACCAATAAATAAATGTGTGAGTGAGATGGTGAGTAACTGAGTA
GAGGAATGAATTAGTGAAGAATTTAGTGAATACGTGAACGAATGAAGTGTGAGTATGAATGAACGGCAGG
ACGGAGCAAATCCATGAATAGCTGTCTGAGGGCGAAAGCGAACATGTGAATGAATGAGAGTCACAAACGA
AGACTTAGTGTGTGAGGGATGAACCGGCAGAGCGGACGCTGACCCCGGTCCTGCTCCCACCAGTCAAGCC
GGACCCTCCGGAAGGCGTGCGCCTGCGCACAGCGGGACAGCGCCTGCAGGTGCTCTGGCATCCCCCTGCT
TCCTGGCCCTTCCCGGACATCTTCTCTCTCAAGTACCGACTCCGCTACCGGCGCCGAGGAGCCTCTCACT
TCCGCCAGGTGAGCTGCGCGCGCAGGGGGGCGTGGTCTGGAAGAAGGAGGAGCACAGAGGTGGATGCTGA
GTGAGACTGGGTCCCACTGGGTAATCTCCTGTCTCCGCCTATCGCCTCTGTTGGAGCGTAGAGATTGTTG
GTGTGATTCAGCAATAGACTTTACCTGCTTGCTGGGAATTCGAACTCACTGCAAGCTCTTTACCCACCAA
GCCAGCTCCCCAGCTCCCCAATGCATTGTGGTCCTTCCCACCTCCTCCTTCCAATGGCCTGACACCCCTT
CTCCCCACAGGTGGGACCCATTGAAGCCACGACTTTCACCCTCAGGAACTCGAAACCCCATGCCAAGTAT
TGCATCCAGGTGTCAGCTCAGGACCTCACAGATTATGGGAAACCAAGTGACTGGAGCCTCCCTGGGCAAG
TAGAAAGTGCACCCCATAAGCCCTGAAGAGGGACTGGATCCTTGATATCAGACCCTGATGTCGTCTACTT
GAGGGTGGCAGGGTGGCCTGTCCTGAGTCTGAATACTGACTTTCCATGTACTGGGCTGCTCCGAAGCACT
GGATAATTCACTTGACTTCTTCAGACCTCAATTTCCAACCCTGTGGGATGATCTTTCTTCTTCTGCCGGT
GCGGGGCTTTGTGAACTTTGTGATTTAATTAGAAAGACGGATTAAATAAGAATCATCATC

Protein Sequence : Show Sequence

>gi|7657052|ref|NP_056581.1| interleukin-27 subunit beta precursor [Mus musculus]
MSKLLFLSLALWASRSPGYTETALVALSQPRVQCHASRYPVAVDCSWTPLQAPNSTRSTSFIATYRLGVA
TQQQSQPCLQRSPQASRCTIPDVHLFSTVPYMLNVTAVHPGGASSSLLAFVAERIIKPDPPEGVRLRTAG
QRLQVLWHPPASWPFPDIFSLKYRLRYRRRGASHFRQVGPIEATTFTLRNSKPHAKYCIQVSAQDLTDYG
KPSDWSLPGQVESAPHKP

Molecule Role : Vaximmutor
Related Vaccine(s): MVA/IL-15/5Mtb vaccine

10. Fth1

Gene Name : Fth1
Sequence Strain (Species/Organism) : Cavia porcellus
NCBI Gene ID : 100379547
NCBI Protein GI : 290491181
Protein Accession : NP_001166318
Taxonomy ID : 10141
Chromosome No : Un
Gene Starting Position : 9448211
Gene Ending Position : 9449938
Gene Strand (Orientation) : +
Protein Name : ferritin heavy chain 1
Protein pI : 5.3
Protein Weight : 20567.49
Protein Length : 182

DNA Sequence : Show Sequence

>gi|347623380:9448211-9449938 Cavia porcellus strain inbred line 2N unplaced genomic scaffold, Cavpor3.0 supercont2_65, whole genome shotgun sequence
CGCGGTCTCCTGCTTCAACAGTGCTTGGACGGAGCCCGGTGCTCGCCTGCCCTTACCGCCGCCCTCACCG
CCGCCGCAGTCTAACCTCGCCCGCCGCCACCACCACCACCGCACCGCCGCCGCCGCCATGACGACCGCCT
CGCCCTCGCAGGTGCGCCAGAACTACCACCAGGACGCCGAGGCCGCCATCAACCGCCAGATCAACCTGGA
GCTCTACGCCTCCTACGTCTACCTGTCCATGGTGAGCGCGTGGCGTGCAGCCCGCAGTGGGCGCTCGGGG
TTGCCTGGCGACAGCGCAAGCTCCCCAGGGACGGGAGTGCGCGCCTGGATGTAGGAGAGGCGGCGGCGCC
ACTGACCCCCCTCGGCTGTCTCCCGCCGGGGCGTGCACTTTGAGGGTGCAGCTGGAGAGCTCGCGAACCT
GTGTAGAAGATACCGTGCAGGGCGAACGCTCAACGCACTCCCTCTAGACAGGAGGATTTCCCTGTAAACA
CTGCTGGAGGTGATTTTCGGTGCTAGTTTGAAGCTTCTTGAAGCAGCTCTCAGCGACCAAGTCCCAGTTT
TTTGACACAAGATCTCACTGTGTAACCCAGGCTGCCCTTGAATGCACAGTTATCCTCTTGCCTCAGCCTC
CTCAGTGCTGGGATTACATGCGTGCTCTATCAGTTTTCTAGTTTAGAGTTAAGAGCCTAGTCCCTTTCAG
GAACACATCAGGCCCATTCAGCCCTGGCCCCTCTGGTTGTGTGGTGAGAAGGGCAAGCTGGACAGGCAGA
GGACACTCACCACCTCCCCTCGCGACAGCCATCTCCCTTCCCATGGGAACCTTCACTGGACGGTGATGTT
TATTTTCAGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTCGCCAAATACTTCCTTCACC
AATCTCATGAGGAGAGGGAACATGCAGAGAAGCTGATGAAGCTGCAGAACCAGCGCGGTGGCCGGATCTT
TCTTCAGGATATCAAGGTGAGTGGGGAAGGTCTGGATGTGAGAGACTCTAGGGGTCCTTCCCTCCCCTCC
ATGTGCAGCTATGGCTGGGGCTCTCTGCCTCTGTGCGTGTCAGGGCCACTTTATGCTAATGTAGGCACTG
AATCTGCAAGGACAGTGAAGCAGCAGCTCTAGAGGGGAGATGTGGGCATTAGACCCCAGCACTCACAGAC
TGGGGCTGGCCCTGTTGGGGGGAGTCTGGGCACTGGTTGATCTGGTGCCTTTGCTCAGAAACCAGACCGT
GATGACTGGGAGAATGGGCTGAATGCCATGGAGTGCGCCCTTCACTTGGAGAAGAGTGTGAATCAGTCAC
TCCTGGAGCTGCACAAGCTGGCCACTGACAAGAATGACCCTCATGTGAGTACAGGGATTGGGCTGGCAGC
AGAGGGAGGTGGGGTTCACGAGGCACCGGGAGCCAACCATCTACCTCTTGTCTCCTAGCTGTGCGACTTC
ATCGAGACTCACTACCTGGATGAGCAGGTGAAGGCCATCAAAGAGCTGGGCGACCACGTGACCAACCTGC
GCAAGATGGGTGCCCCTGAGTCTGGCATGGCCGAGTATCTCTTTGACAAGCACACCCTGGGGGACAGTGA
CGAGAGCTAACGGTGCTTGGGCAGTGTGTGCATGCTGCGGCTACCTTTACCTTTTCTATAAATTGTACCA
GAACATCTAAAGTTCTTTAATTCCTTCAAATAAAGAGATTTGGTACCC

Protein Sequence : Show Sequence

>gi|290491181|ref|NP_001166318.1| ferritin heavy chain [Cavia porcellus]
MTTASPSQVRQNYHQDAEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKL
MKLQNQRGGRIFLQDIKKPDRDDWENGLNAMECALHLEKSVNQSLLELHKLATDKNDPHLCDFIETHYLD
EQVKAIKELGDHVTNLRKMGAPESGMAEYLFDKHTLGDSDES

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

11. Ftl

Gene Name : Ftl
Sequence Strain (Species/Organism) : Cavia porcellus
NCBI Gene ID : 100379558
NCBI Protein GI : 290491214
Protein Accession : NP_001166329
Taxonomy ID : 10141
Chromosome No : Un
Gene Starting Position : 8357696
Gene Ending Position : 8359000
Gene Strand (Orientation) : -
Protein Name : ferritin light chain 1
Protein pI : 5.89
Protein Weight : 19110.14
Protein Length : 175

DNA Sequence : Show Sequence

>gi|347623385:8357696-8359000 Cavia porcellus strain inbred line 2N unplaced genomic scaffold, Cavpor3.0 supercont2_70, whole genome shotgun sequence
TGCTGTGAAAGGCTTTATTTTTCTTTTGGTCCAAGATTTGGGAGGGGACTCCAAAGAGGTGAAAAAACTG
CCTAGTGACTGGAGGGAGAGGGTCCAGCCAAAGCCCTGATGCCAGTGATGCTGGAGCCCCTCAGATACTG
CCAGGCTCTGGGGCCTCCTAGTCGTGCTTAAGTGTGAGCCTTTCAAAGAGGTACTCGCCCAGCCCAGCCT
GGGGACCATCCAGCCTGCGGAGGTTAGTGAGGTGGTCACCTATCTTCTTGATGAGCTTCACTTCCTCATC
TAGGAAGTGGTTCTCCAGAAAATCACAAACCTGAAAAGCAAAAGAAGTCAGAAATCCAATTCTGGGCCAA
GAGGGTGGAGCAAAGCCGTTTCTAACAATGGGACATGCAGGAGGCAGGCTCAGAAGTAAATGTGCCTACG
GCTCCTGGGACAGTCACTTACATGAGGGTCTGTCTTGGCAGAGCCCAAGGCATGGAGATCCAGAAGAGCT
TGGTTCAGGCTCTTCTCCAGGGTCAGAGCCGCTTCCATAGCATCAAGGGTCTTACCCCACTCATCCTCAG
AAGGCTTCTACACAGAAAACAAAAGGCACAGAAACGGCCACGTTATCGTACACACTCACAGAGAGCTAAA
ACTACAAATCTCAGTTTGAGTGATCCTTTCCTGCAGTAACTTGCACAGCCTACACCTGATTTCCCAGAAA
TCCCAGCGACAACTGATCAGCTAAGGCCCAGGAGGAGCCCCATGGCAAGGACTGAAGTCGCTACTTCAGT
CCTTGTTTACCTGCACGTCCTGGAAGAGCGCGCGGCCGCCGCGCTGGTTTTGCATTTTCAGGAGGCGCTC
GGCACCCTCACGTTTCTCCTTGGCCAGCTCACGGAAGAAATGGCCCACGCCCGCCAAAGCCACATCGTCG
CGGTCGAAATAGTAGCCCTGCGGGAAGAGATGGCGGGAAGTATGTGGAGAGTTGAGGCCAGGCAAGCGGC
CTCCTCCCTCCGCCTGCCCCGCAAAACAAAGACGGCCTGCGCATGCGCACAGCCAACAAGTGCGCAGTTA
CGAGGAAACAGGGCCTAGGGTGTTTGGGGGACCCTTACCAGAGAGAGGTAGGTGTAGGAGGCCTGCAAGT
GCAGGTTCACCAAGCGGTTGACGGCAGCCTCCACCTCGGTGGAATAATTCTGACGAATCTGAGAGGTCAT
TGCTGATCAGCGGGAAGGAGCTACCCACAAAAGAACGGTGTAGGCTGGCCCTGGAAGGAGCAGATGGTCG
AGAAGATGGTCCCAAAATTTGTACCTGGAGAGTGGATGGGAGATT

Protein Sequence : Show Sequence

>gi|290491214|ref|NP_001166329.1| ferritin light chain 1 [Cavia porcellus]
MTSQIRQNYSTEVEAAVNRLVNLHLQASYTYLSLGYYFDRDDVALAGVGHFFRELAKEKREGAERLLKTQ
NQRGGRALFQDVQKPSEDEWGKTLDAMEAALTLEKSLNQALLDLHALGSAKTDSHVCDFLENHFLDEEVK
LIKKIGDHLTNLRRLDGPQAGLGEYLFERLTLKHD

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

12. GPR182

Gene Name : GPR182
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 11318
NCBI Protein GI : 6005705
Genbank Accession : AC026120
Protein Accession : NP_009195
Taxonomy ID : 9606
Chromosome No : 12
Gene Starting Position : 54427153
Gene Ending Position : 54444913
Gene Strand (Orientation) : +
Protein Name : G protein-coupled receptor 182
Protein pI : 7.55
Protein Weight : 41850.38
Protein Length : 404
Protein Note : Also known as AMR; 7TMR; ADMR; AM-R; G10D; gamrh; hrhAMR; MGC34399

DNA Sequence : Show Sequence

>gi|157704453:54427153-54444913 Homo sapiens chromosome 12, alternate assembly HuRef, whole genome shotgun sequence
CCAGCCTCCTCACAGCTCCCCATAGCCTGGACCTGCCGGCCCTCCCTCCAGGACCGAGGGGCTCCCAAGG
GAAACTCAGGTGAGTGTCGCCCTCTCCCAGGGAAGATGGGAATCAGGGCTTGGAGACAACTGGCTATCCT
GCATGATCCACAGGTTTTCCTAGGGATGAATCAGATTGTGTGGTGTGCGTGTGTGTGTGTGTGTTCCACA
CTCACGGTCCAAATAACAAGACAGAAAAAGAGCAATAAAGATCTGTGCAAAAGGGCTGAGGAATGAAGTG
CCAGGAGAGAGGTGGAGGTTGGAGACCACCTAGGAATGGTGGGTGTGGGGGGATTGAGGGGGTTGTTGAG
GGGGAACTGGCTGGGGGTGGGGAGAAATGTTGGTGGACACCAACAGTCCTGGCCTCTTCCAGCTGAAACC
CCTTAAGAAAGTATCTCTGCTCTTGGGTCAGAGCTAGCAAGTGAGTTGTGTATTATTTATTCACAGTTTG
AAAGCTAAACTTCCCTTTCTTGCCTTAGCCCCAGAATCTTTCCCCAAAGCCCCCGACTCCCTCTGGCCTC
TACCTTGAGGACACAAGGCTCTCGAGGTCTGCTTTCCCTTCCAACCCTCCCTTCATCCCAATAGGCGTGT
GCTGGTCCCAATGTCAGTGAAACCCAGCTGGGGGCCTGGCCCCTCGGAGGGGGTCACCGCAGTGCCTACC
AGTGACCTTGGAGAGATCCACAACTGGACCGAGCTGCTTGACCTCTTCAACCACACTTTGTCTGAGTGCC
ACGTGGAGCTCAGCCAGAGCACCAAGCGCGTGGTCCTCTTTGCCCTCTACCTGGCCATGTTTGTGGTTGG
GCTGGTGGAGAACCTCCTGGTGATATGCGTCAACTGGCGCGGCTCAGGCCGGGCAGGGCTGATGAACCTC
TACATCCTCAACATGGCCATCGCGGACCTGGGCATTGTCCTGTCTCTGCCCGTGTGGATGCTGGAGGTCA
CGCTGGACTACACCTGGCTCTGGGGCAGCTTCTCCTGCCGCTTCACTCACTACTTCTACTTTGTCAACAT
GTATAGCAGCATCTTCTTCCTGGTGTGCCTCAGTGTCGACCGCTATGTCACCCTCACCAGCGCCTCCCCC
TCCTGGCAGCGTTACCAGCACCGAGTGCGGCGGGCCATGTGTGCAGGCATCTGGGTCCTCTCGGCCATCA
TCCCGCTGCCTGAGGTGGTCCACATCCAGCTGGTGGAGGGCCCTGAGCCCATGTGCCTCTTCATGGCACC
TTTTGAAACGTACAGCACCTGGGCCCTGGCGGTGGCCCTGTCCACCACCATCCTGGGCTTCCTGCTGCCC
TTCCCTCTCATCACAGTCTTCAATGTGCTGACAGCCTGCCGGCTGCGGCAGCCAGGACAACCCAAGAGCC
GGCGCCACTGCCTGCTGCTGTGCGCCTACGTGGCCGTCTTTGTCATGTGCTGGCTGCCCTATCATGTGAC
CCTGCTGCTGCTCACACTGCATGGGACCCACATCTCCCTCCACTGCCACCTGGTCCACCTGCTCTACTTC
TTCTATGATGTCATTGACTGCTTCTCCATGCTGCACTGTGTCATCAACCCCATCCTTTACAACTTTCTCA
GCCCACACTTCCGGGGCCGGCTCCTGAATGCTGTAGTCCATTACCTTCCTAAGGACCAGACCAAGGCGGG
CACATGCGCCTCCTCTTCCTCCTGTTCCACCCAGCATTCCATCATCATCACCAAGGGTGATAGCCAGCCT
GCTGCAGCAGCCCCCCACCCTGAGCCAAGCCTGAGCTTTCAGGCACACCATTTGCTTCCAAATACTTCCC
CCATCTCTCCCACTCAGCCTCTTACACCCAGCTGAGGTAGAGGCCAGACTCCTCCAACAGTGAAGGAAAA
GGCACAGGTGAGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGATAGCCATGGACTCTGGATCTTTA
AAAATCTGCACTTCCAGCTGGGATTGGGAAGGGAGCTGAGGCTAAAGAGGTGCTCGGCAGAGGAAACTAG
ACACATTCATTTCATAAATCACTCTGGCTGGAAACAGTGAACTCTGCAGGAACCTCACAAAACTCCTTTT
GCGTTTGTGAGCTAGATGATCTTCCAGCTCACTATGCAGGTTATGGGGACAAGAGGCCAGAGGCCAAGGA
ACCAGGCGGCCCCAGCAGGACGTTCCCTAGCTGTGCATCCTATGCACAGCCATGATAAGTGAAGGCGGAT
GGGGATGGCAGGGGTGGGGGCGAAGCCAAGGTCTGAGACCTGCACCCTGCAGCTGGGTCAGCAGGATGGT
ATCTCCACCCGCGGCCATCCAGGAAACACAGCTCCATTTCCTTGCTGACACCCTCCCCTCCCCGTTGTCC
ATCCTGCTCCCTCCCCACAAGCAAGAGGAGTTGTCCCAGAGTCAGCTCCTCATAGCTGACCTCTTACCAG
CTTCGGATCTAGGAAACTTCCAGACTTCTAGGGAGACACAGAGCTACCAGAAAGCCCAACCCACCCCCAT
CTCTTTCTCTCCAGATCATAGGAAAGTATCAAGGGATGTCATTCCGGGAGCAGCCCTTGGGACAGCCAGC
AGCAGGTGCTGGCAGATGCTCTGTCCTTCACCGTCTGTCCCCGGCACCAGATGGGGAAGGCCACAGTGGA
AACTTCTCAATTCCGTCACAGGGCAGAGCTCTGACACTGTTATAAGTCAGTTCTCCCTGGCCATGTCTTT
TCCTAATTTTCCTGTCCCACAACTTAGGTTCCTTAACACTTGTTCTACAGATGAAACAAAAAAGTCCTCT
GTTAACAAGTAGACCTTCTGGAAAAGGATCACTTATGTTTGGGAGACTAGGACCCTGTCCATGAAGAACT
CTGAAGTAAAGGCCTAAGAACAGGATCTAGTTTCCGCCTTCCAATTCCACGTCCTTTGCCTCTTAATCCT
GCCCGTGGTAAGGGGACTGGATGGAATCACTGATAGCCTCTTCACCATCTCCTCTGTCCTGCTTAAGTGC
TCCATGTGTCTGGAATGTTTAACTTGGAGTAACTACTCACTGACTTGGAGACCACCTGTTCTACTTTGAG
AAATCAGAACTGTTCTGTGGATGCACTGACAGCGTGCTCCAACTGCATATGCACCCAGGTTTGTCAGGAG
CATGGAAGGAAACCTGCATCTCCCTGTTTACTGGGCCCAAACCTGAAAGGGTCTTAAGCTGCAGACAGCT
TCTCAGTAGCACAGAGCTGAAGGCTTTTTGAACCAGGAGTCTGGGTTGTGGCTTGGGTCGCAGTAAGGGC
TTAGCCTATCAATGCAGAATTCACATTGAAAAAGACTTTCCTCTCTTGTCCCAACACAAAAAAGCAAGCC
AAGATCTGATGCCCATATCACTGCCTCCTCTGACTTGGGCTACAGGAGTTTCCTGAAGGAAACCAAATTT
TACATCTCTGTCACATTCCAGATCACCTTAGAGGCCACGCTCCCAAGATTAGTTATCACCCTGGCAGTAT
GAATACTTCCCTAAGGCCTCCCATCCATGGAGGGGAAGAGTGGGAACCAGCTGTTACACTCAGCATCTAC
TGAGCACTGATGGGAGCCCTGCCTGGGCCATGTGCTGTGGGGCCAAATGGACATCACTCCCTAACTTCTT
GAGGTCTGTCTCCTCTGTGGATTCACGCATGTATCAACCTCATTAAACTATGCCAAACTTTTGCCTCCTC
CCTGTTTCAATCTGCCTCGCTCCACTCAAAGACACACCATGTCCCCGCAAATCAAATGGCATTGGCAAGA
CAAACTGTAGCCAAATCTCTCACTTAGTAACGTTAGTCCAACTAGACACGGGCAGGGACCCACCACTCTC
TCTGGGCTGACGGAGGATCCTCTCAGGGATACCCCCTGAAAAGTTTGAGTCTTTGACTTAATTTCCCACT
AAAAACAGGTCAACAAGTTAATCCTGGGGAATTCCAGCTACAGGAAAGGAAAATGTGCTACCTGAAAGAA
TTCTGGCATCTGCTGGGCAGAAATGTGCTGCTGAAAGGGTGATGAGCCCAGATATTCTGAACTCCAGGTA
TGAGCATATTCATAGTAGGTAGGAAGAGAACTCTCATTTCAAATTTCTTTTTTTATTCTAAATAAAAACC
ACACTTTGTGCTGAACAATGGAATATAAAAGAATCAGATGTACAATGATTTTAGACATCTACTATTTGGG
GAAAAAAGTTTACAAAATATACAAAACTTTACAGCAATAGATAGTAAACACTTAAATATTAGGAGGTCAG
TACTTATTAATTTAATGGAAGGAGTTAGACGTCAACAACTCTTCTCTGTTTCATAAGAGACTTTAGCTAT
TCAAGGTGGGGGCGGCGAGTGGATTGGACAGGGGAAGGGTTTTAAAGAAACATCAAAATCGAGTTTCATT
TTCATTCTGATGTTAAGCTTATGATGCATAACCACATTGAAACTAATGCTGTGCACCCTGCCTCCTGACT
CATTTGGTGCAGGATTTAGGGAAAAGAGTCCCAGATGGGTGCAGACCACCCCATAGAGCCCACTCCCATA
CCAATAAATACAGTGTCTATCTAGAATTTGCTTTGGAAGTTAAGCAGTCTTGGGCTTGGGGAAAGGGGCA
CAGAAGGAGGCCAGAGGAAGAAGAAAATTCAACACTAGCAAAAACCTGTCTAGGAAAAAGATATTTAAGC
CCAAAGGTTTGGCCTGGAGACAAACAGGTACTCACAGATCAGAAAAGCAGAATATAAAAAAAAACTTTTA
CCCATCTCCTGGTTCTTAGGGGTCACCTGGGCCTGACCCTTATCCCACCTCCTTTCTTCCTAACTAGCCA
TGGACTACGGGAACTCCATGGAGTCATAAAATTTAGGACCAAATACTAAGGTTCAGCAGAGCTTCCTAGC
GGGTGCTGTGGCATCAGTGAGTCACCCAGTGGGTCACTGGAGTGCCAGGATACTAATCTCTGCCTCTGGC
CACATGGAGCCTCTGCTGTTTAACCCCGGGTGCCACACAAGTATTACCATTTTCCCCTCGGAAAACAAGT
TGGGAATGGCCTCATATTTGGTTTAATGCTCTATGACCACCACCTTGAAATTTTTAATAATTTATGAATA
AGAGGGCTCCACAAATGATGTAGCTGGTCCTGAGTACATACCAAGAAAATGGGAGAAGAGGATGTTCAAG
GCTTAAACAGAACTTCAGAGTAACACAGAAGAGAGTAGTAAACTAGGTTCCAGGAGAGAGGGAGCAACAA
AATCAAATGAACAGAAAGTCAAAACAATTAAAAATGCAAAATAAAATGAATACCTTCTACCAGGTATCCC
AGGGTGCCCTCTGCCACAATATAGTGTTTCAAGCAAAACCATTCAGAAGAGGGTGAGCTGGAGGCAGAGG
AAGCAGACATGACTTTGGTGGGGTGGGGGGAAACGGGAAGTTCAGGTGTGAATACAGGGGTCACCAACAG
GGAGAGGGTTTGGTCAACAAATCTCATGAGCTACTGATCCTATGACGTATCTGACTGGGACCAACTGAGC
CACCCTGATCAATCTGATCAGCCCCCGGGGGATCAGGGGATGCAGTTTTCTGTTTCGTGTTTCAAGGGCC
AAGTTTCAAGGATCTGCTTTTTGGTTTACTGGCAAAAAGGGAAAACTTCACAGTTTCAGGTTAAGTACAC
AGACCACCCAGCATCCATGATGCCCTCTGTTAAGGAAAAAGGTTTCTCCAGCACATTTATCTGCTCTCAG
CCACTCGGCTGGTTCCTGTAATTACCACCTCTCTCACACCAGCATTTCAAAAATCAGTCCTCACCAACAG
TCTGGGAGGGATTGCTCATGCTTGAAAATGTAGCAGACACTTGACTGTAGTCTGTTACACATTTCCTTCT
TAAATTCTAATGGTCAGTGTGAGTAAGAAAGGGAGAAGCCAGTCAGCAATGAAGAGTCTCAACACTGCTT
TCCTGATGAGGATGTCCTCAGAGGACACTGAACAAGGTGGGTTTAGATGGTCAAAGGTGGACCTTTAAAC
TGGCCACTGTACAAGTCCCTTCATCTAATGGTCACCCCACCAGAGAGCCAGGCACTCCAACTCTGAGGCT
TTATCAGTTCAGGGCTGGGGAGAAGCAGCAGGTCCAACAAGGATGGAGCTTTGGTTTGTCTGACATCTCA
ATATAAATAAAGACAGGAGGCTTCCAAACTGTGCAAATAGGTCATTCATGTCACTTGTTCCCCTCAACCC
TGTCCCCAATCTACTCCATCACAGGAAGGAAGGGGGATGAAAGGCCTGGAATTTAACTGTCCCGAACTTT
ATAGAACATACACCCATGGCCTAAAGTGTTCCCTGGGTTTATTATGCTAGAGACAATCTGGAGAAGAAGA
ATTTGCTTAAGGGCTTGACATGTCTTCCCTTAGTTATACCCCTGGTTCTCCACATCTCTGGTCAAGGCTG
TTCTCTGGAATATGGCTACTTGCTGGGGAGAAACTCATTTTCCTAAAGTCTCTTATACAAAAGAGATGCT
GGGTAATGTGTATACAAAAACAAAAATAAATAAATATTAATGTCCAAAAAGGCTTTGTGAGTTAATGAAA
ACCACTTGCTTGTGAAACAGCCCGGGGTGTTGCTGAATCCCACCAGGGGCAGTGCCAGGGCTGAGGCCAG
GGACTCCCAGAGCTCACCTGAGCAGGGTGAGGTAGTATGAGCTACTGAGGTGGCACCTCACAAGTATGCA
AGATCGAGGGCCCTATCCCTCAAGACTTCTTTGGGCAAAGCCCCTTAGAAAACCAAGTAAGAGTGGAGCT
AACCAGCTGCGTGGGCTGTGCCGGCACACTCTGCAGCCACCCACGGAGGGCCTGAACCAGCTGCCATCCA
ATAGCGCCCCCTGGAGGTGATGGCAACGACAGATCAGGATAAATTCCAGCAGGTCAAAGGTGAGCTGACA
AAGCTCCGGCACTGTTAGCACAAAGTAAACAGGTTACCCGACTAGGACAGACAGTCAGAACGCACACCCT
GCTACACACCACCAGTTACTCAGGTCCCTTTAATCCTGAAGGGCTCGCGGTCTGCCACTCCACCACTTCA
TGGAGTCAGCTCATCTACACCACGGGCCAAACACTTCTGGACGGGAGACCAGATATAAATTAAGGCAAGG
CTGCTGCTGTCAGAGACATCCCCACCTCTCCACCAAGACACTCATGGCCAACAGAAGTGGCCAAAACAGA
TGACTATATCAAAGTGGTTCCCTATCCTCTCTGCCCCACAGAAGGCCGGACGTCCCGGGCCATCACTCCA
TAAAATCCTGTTTCTTTGGAAATGAGGAGAAAAGAGAAACCTCTCCTGTCCATCCAGGCTGCCACTTCTG
GTGGCACTGCAGGACACCCCTTCTGGCTGGCAGGGGTTCATCCCAAAGCTTTCTGACCTAAATAGAAAGC
TCTGTGCCTAGTATGGATCTAGCTGCCAAAAGATTTTCACTGTTCAATCAGCAGATTAGGGCTGTAGATA
AGCAGACACCAACATCCCTGATGTCTTGTCCCTGCTGAGGGCTGGAACCTGAGGGGACTGCCCTGCTGAC
CTGTCTTTTCTGAATGATCACATTCCCAAAATCTTTCTCATTGCTGGTCATCTCAGTTGGAGGCCTGCAG
TAACACCCACAGTGAAAACCTCCCTTTACCTCCTCTGTTAAGTGAGGCTGTTGGTTGGATTCTTAGCAGC
ATAAAACCTATCAGTAAACTAAAACTAGAGAGGCTGGGGAAGGGGGTCTAGTGGACACCAACTGCTTGAC
TAGATCCCAAAACCAGCCTCAAAAGAATGAAAAACATAGCCCTGCATGGGCAAGAACAGGTATGGAGGTA
AATGTGATAGGGCTGTTGCTTCTCAACAACAGTAACAGCTTATGTGCTATTTCTTCTTCTTTTCTTCTTT
AAACACAACACAGAACAGAATTCCTACCAAGGCAAAATTATAACTTCAGCTGGGGAGGGACCAACACCTC
TTAGATATCAGCATCCTGGCTGCTGCTTGGGAGCTCCCCGTGGCTCTGCCGGGACCCAGTCAACTGTCCG
GGTTCTTATCCGCCTCTTTGGAATGGATGATGTACATGAAGGTCTGCTCGATGGTGAAGTCAGGGGGGAG
GCTGCCTTTGTGCACACCAACATGTTTGCTCATGAGGTTAAGGGTGGAACTGTAGTGCCCACAGACTGTG
CAGCGGTACATGAGGGCCCCCGAGTGTGTCTTTAGGTGGCACTTGATGGTCGAGCGGCCTCGAAAGAACT
TGTGGCACACCTTACATTGGTATGGCTTCTCCCCCGTGTGGATCCGCCGGTGGTAGTTGAATTCGCTTGT
GTGGGCAAATCTTTTGCCACAGACCTTGCAGCTATACTTGCTGTTCCCAGGTTGGCCCTGCAGCGCCAGC
TCTTCACCCAGAAACTCCTCTGCTGGCAGCTTCCGCTTCTGGAGAGCTGGGTGCTGCAGCCCAAAACCAG
GCCGTGCATCAAGGCCACTGTTGCATTTGTGCTGGCTGACGTGGTAGCGATAGGCAGCCTCTGACTTGAA
GCTCTGGCTGCACAAGCGGCAGTGGAAGAGGGCGTCTTCCAGACTTTGGTGCACAGCCATGTGCTTCTCT
AGAAGATGCCAGTCCACAAAGCTGTTCGCACAGACAGAACAGGAGAAGACTGAGATGCCGAGATGGGACA
GCGTGTGCCACATGAGGCTGCAGAGGTTAAGGTGACGCTGGTCGCAGACACTGCAGGCCTGGCCCTTCAA
GTTTAGATGGTCAAGGATGTGGCCCCGGACCACATGGAAATCTTTGGCCAATACTTTCCCACAGGCAGCG
CATTTCAGCTCTGGGGAGGCTGCCCCTGAAAAGAGACCCAGCTTCCCTGGCAGGGGATCGTTGGGGTGGA
CAATGATGTTGTTCTCAAATATTCCATCCCGTCGGTGAAGGGTCAGCTGCCACTGGGTAAAGAACTTAGT
TTCACACATGTCGCAGGAGAAAAGGAAAATACCAATGTGGGACAGGACATGAGTTACCCGGGAGTTTCGG
TCCTGGAAGTGGGTCTCGCAGACCTTGCAGTTGCCCGTCAGCAGGTCCACATGGTCCCGAGCATGCTGCC
GGATCAGTTGAATGTTGGGCTCTAGAACTTTCTTGCACACCTGGCAGGGCATGGCCTTATTCTCCCGATT
GTCATTCTCTCCAAAAGCCAACTCATCCTCACTGTCATCACTCAGCTCAATCACCTCCTCAGTTGTGCCT
ATATCCTCAGCAGGGTCTTCAAACTGCAAGTCATCATCCCCCAGGTCCTCGAGCTGGAGCTCATCCATCT
GCCCAAAGCCAGGATCTTTGGAGTGCTCACTATTGCTCAGACAGGAGTTGGTCCCAGTGGTAATGTCTAC
TGCATTGTCAGGGGTGAGGAAGCTGTTTTTACTGAAGTCTCCATTGCTTTGAACTTTGTATGGCTGGCAG
GAGCTCGTGCTGGTCTGGATGCCCGTGCTGACAGTGCCTAGGAGTGGCTGGGTCATCATGCTAGGAATGG
ACGTGAAGGGAGCAGGGGTGTCATGGTCTTCTGTCTTTGGCGGTGGTGGGGGCGGTTGCGGCAGATGCAG
GCTATGGTTAGCGTCACTGGCAACATTCTTCTCTTCCTCTTTATAGCTCCCTCCAGCATCACTGGGCAAC
ACATAGTTTCTATCAGCACCAAGGTAGTCCCCACCACCTCGGAGTTCTCCAAGGGGATGGGCAGGTTCTG
CCGAAGGACAACTCAGGGTACCAGAGTGGCTCTCACTGGTGCTGGTCAGGGGCTTGGCCCTGGCAGTGCT
CTCCAGATCAGGAAAGGTAGAGTGACAGGCCTGGAGGAGGTCCTCCATACCCAGACGCTCAGCTACCTCG
TAGATGACCCCAACATTGATCAGGTCTGTGAAGAGTTCTGAAGTGTAGACAAAGCTCAGAACCTTCTCAA
AGTTGGCAGGGGTGATGAAGTCCACCACATAGGTCCTGGCAGCATCAAGCCCAGTATTCAGGAAGAGGTT
CTGGAAGTAGCCAGCTGCACAGGCCAGCACAGCCTTGTGGGCCGGGAAGGAGCGGCTCCCCACCACAATG
GTGACGTCGCACATGGTCTCTGAGAGCCGGCACTTGTTGAGTTCCTTCAGCAGGTTGTTGGGGTGGTTGG
TGCTTTGCAGTTTGATCCTCATGCCCATCTTAGCAGCTCCTATGAAATTACCTCCTTATCAGCACAGTTA
ATCTGTGGATAGCAAGAGAAAAAGATGGATGGCCAAACACATCCGTGCAAAAGAGGGGCTATGCAGGAGA
AAATACCATCACTCTGAATGCTTCCAAGCCCCAGGTAGGGATCAGGAGTAAACTGGCATGGAGAGTTCCT
CCATTATGACAAGGCAATGACATCTCCCCCTGTATATACTCAAAGAGTTATGACAAAAACCAAAACGACT
GATGACAATCTGATAGCTTGGTTTTAAGCTGGTGCCTAGAGACGAACCAGAGGCATTCCCTTGGACACAA
TCTTATACTTCATTACCAGAGCCACTGGATCAAATTTTTCTCCTATTAATTTCCTCCCAAATACAAGCTT
TCCTAATTCATTGAGCATATTCTTTTCCCTCATGATGCCAAATACCATGCACATGCTTGAACACAACTAT
CAAAGCTCCACTAGCTACTGAGGAACATATAAGGTGGTTCCGTTTTTTAGCTTTGTCATTGCTGTGTCTT
CAACCTCTCAATAATCTCCAAATAAATCTCACGTTCTTGAATCCCCATCTCTCTCTGCCTATACTACATA
CCCGCTCTCTGGCCTTTCGATAACCCAGAGCCCCTCCAGATATTGCTTTATCACAGTCCCTGTATTCTGT
GAAGTTAAAACACTCAGAATGCACAGTAAGAAAAGTAGAACACAACTGTGGTAGTATTTAATATTACTTT
AATATGTTGTATTCTTTTTCGCCACCCTTTTCGGGGTTTATATTTTGTTGTCCCTGCACTAACAGATTTA
CTTGATGCCGCAAGAAAGACTTCTAATTATACAGGTAAGACTCCTAGAGGCTTGTTCACTACCACTTTCT
GCCCTTTCTGACTCCTGCACGACTAAGGAAGAAGAGCTACTTGCATCCCTGTTCAGTCTCACAACTCTGC
CCAACACAGCCCCTTTCTCCATCTCTCCAGATTCTAACAACTCCCAACTTTTTCTGGGGCCCAGGAAGCG
TCTAAGTATTGTGGGGTACCGGCCAAGCATCTCCCGGGCCCTGGTGGCCACCCCTGAACGATGGGCGGGT
CGGAGCACTGGGCAGCCAGAGGAGAGGGTGGGAGAGGGGGAAGCTGGGAACTTCCAAGGACACCCCAACA
ATCCCCGGGCGCCGCGGTCTCCCCTCCGGAGTCGCATCTGGGAGGGAAGGGCGCTGCATCGGCTGGGGCG
CTCCCTCTGGAGGCAACGAGCGCCTCAGTCCCTCCACAAACAGCGCGGGGCTCACCCGGGTGAGCCTGTT
GGGGGCATGGGGCTGAATAAGGGGCCAAGCGAGGGGCGGCGGGGCCGGCCGGGGTCGGCGCGCGGGCGGG
GAGGATGGGTCCGGCCGAAGGGAGCGGGCAAGAGGGACCCCAGCAAGAGCGAGATAAATGCCGGGCCGAA
GGTCGGGCTTCGGCCAGGGGCAGCCCTCGGCCTGGCCGCGCTTACCCGGCTCTGCGGCGCCCCGAGCACC
ATCGCCGCCGCCGCTTCACACAAAGGCCCCGGCCCGCCGTGCCCGGCCCGACGAGGAGGCGGCGCCGCCG
GCCGCGGCCAGACTCTCCATCCGCCGCGCGGNNNNNNNNNNNNNNNNNNNNAAAACCCTATCCAAACTCT
CTTCACCTCCCCTGTTCTACGCTCACAGTTCCAAAAGCTTCTCCTCAGAACATCTACTATGGGCATTTTA
CAAAATGTTGTAAGATTGTTTGATTACTATATATCTCCTCCACTAGGCTGTGGGCTTCCTGAAGGCAGGG
ATCTGTGGCTTTGCACACGGCCGCAGGTCCACACTGACCCTTACCACACTTCCTGGCACAATAAGTGCTT
AATACATTAAGCCTGCTTGAATGAATGAAGGAGCACTTGAGGGCTAGACCGCTACTACTGGACCCTTCTG
ATAATCCACTGCTGTCCCTCCTTCCATCTAGGTGGCCAAATGAAGAAAGTCACCGGATCTCACTGTACTT
TGGCACCCCAGGCCTGCTTCCTGAAGCCTGCCCTCCCCAACTCTGTGTAGAACTCGATCACTCTTCCTCC
CCACCCTTCTCCCAAATCATCTTTTCAAATGCTTCTGCCCAAGAATTGTCATCAAACGAAAACCACCTAC
ACTGCCTGCCCCACCCACCACCACCAAAGACATTAGAAGTCTTCGTCATGTCGCCCTCCCTCCCTCAGTA
CAGAGGAAGCTCTTAGGCTCCAAGACCCTTCAGTTTCATCCTCTCTCCCATCCCATCCCAGTCACTGCCT
GAAAATAGCTTGCAGCCAAGGACAAACTCCCAGGACAGGCGCATGGTAGGATTCAGTAATTCCTTGATGG
ATAAGTGGAAAGGAAGGGACACACTGAATGTTGCCAACAGATCTAAGCCAGTTTGGCCATTCAGCAATTG
TCCATTCTCTTGATGAATTTGGGAGATTGTGTGTCCTCCTTTCCACAACCTCATTAACTGGTGGCAGACT
ATACTGAAAGCAAGCACTATACCAAATGCTGCCAAACATGTAACATTTGTTAGCCAGGAGATACAAAAAC
AGCCATAGAACTGTTCTTATAGTTTGGGGATGAGGCATCTCACTTTACCATCTAGTGAAAGGGTCACTTA
GAGGTTGACACAATGTTTCAGTGGCAGGCCCAGCTCTCACAGTTGTGCAAGGTGACTGAGTCTCCCTAAA
GTGGAATATCACACAGCCAGCAGGTCCAGACCTCCAGCATGACTCTGGAGACAGAGGGCTGCTGGGCTTG
CAAGGGCCTGGGCCTATACAGTCTTTCTAACCAACTGCCCCAGCCATCTCTGGAGGGTTGACCCCAATAA
ACTTCACATGAAAACAAATCATCCAAAAGACGCAGGTGAAAGTATATACCACTTATACTGAAGTCTTTTT
AAAGTAAATCACCATATAGTCACAATAACAAACATATACAAAGTTAAATGGAGAAGAAAGGGCCAGGAAT
TTAGTCAAGGTCTTGTGGACCAGATCTCTTTAGGCATCAACTGTTTTACAACAGGAAAGGGAGGGTCAGG
ATCCCTTCACACATTCTTCAAGTACAAACACAGGAATCTAACTTAACATTTGTCTTCATTTTTTTCTGGA
GTCCCCAGTACTGCAATTTTTGTCAGTCCCACTAGAGAGCAATCTTTCTGCACTTAATGTTCCTACTTGC
AAAGCAATTGTCTCATCTCAGCCTATAAGCCTAACTCCCACCAAGTCTGAGCAGAACCAACTCTTGTAGC
TTTCATGACCCAGAGAAAGCCCCTAGACTCTGCAAAAGTGTCCCCAGTTTTAAGCCCTATAGATAGATAA
TTTTGAAAAGTCAAAGAGATGACTCACTGGAAAAAGGGTCTAGGCTCTGGGGTGGGGGCATTCTTTGACC
TCTGGCTATAAAGCTGACATAAGTAATACTAGGGTGAGTATTCCCTCCTTTCCCCGCCCCCCACTATACA
GCTGTCATTCCAAAGTGGCCCCAAGTCAGGCTGTAGGAAGATAGAATTTTCACTAGAATGAAAGGACTGC
AGGAAGTGGCCTCTGGCTCTGTGGCCCTGTCCTTACACTGCCTTGGGAAATCTATGTGTTGATGTTTTAT
CAGTGCAATTCAGGGGCATAACAAAGGCCCCTGTACCACGCTAAGATAAGGAGCCTGAGGATTTCACTGG
CTGTTCCATTAGGGTACAGAACCCCCCTTAGGATGGCGGGTGATAGTTGACATCATAGATTAGTAAAAGA
CATCCATACCCATGCATACCACATGTAAGTGCCTTACACATTCTGGGGACTGTTAAGTCAGAGCTGCAGG
AGCTGGTAACAGGCACTTTAGAGAGGAGTGGGAGATAAATGTTTTCCCTTTATAATAACTAAGGCAGCCA
GTTAACGGTGAAGATATCTGCTCCTCTCTTTCTTGGCCTCGGTAGTAACTTCCCTGTCCACATCCCATCC
TAGCTGGATCAAGTTCTGTTTGCTTGTCCACAGCCCTGCTAAACTGTAAACTCTGTGTATGAGTCATCAT
TTCATCCCCAGGATCTGTCACAGAACCCAGGCTCAGCATTTGGAGACAAAAAAATTGTATAGGGTCATAG
ATTTGTCCTCCTCTGTGGCTGGGTCTCCGGACAGCCTTGATCCCCACCTCCTTCTCTAATTAAGTAGGGT
CAGATTCAGGAATGAATATCTCCCAGACCCATCCTAGTAATACAAGTCATTCTGAATATTTTTTTTCTTT
TTCTTTTTCTTTTTTTATTGTTTTTTTTGTTTGTTTGTTTTGTTTTGAGACGGAGTTTTTTGCTCTTGTT
GCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAACCCCTGCCTCCTGGGTTCAAACGATTCT
CCTGCCTCAGCCTCCCAAGTAGCTGGAATTACAGGCATGCGCCACCATGCCTGGCTAATTTTTGTATTTT
TAGTAGAGAAGGGGTTTCACCATGTTGGTCAGGCTGGTCTCGACCTCCTGACCTCGTGATCCACCCGCCT
CGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGGGCCCGGCCTGTTTTTTCTTTATCTAAAAC
TGGTCTGTAAAAATCTTTGTGTGTTAGAAACCATCTTCCAAATTCGTGTATTTATTTTAATCACTAAATA
AATCTGATACTCATCGTGCAAAGCACTGTGTAAGGCACTGTTCAGGAAATAGATATATACAAAAAGATAT
GTAAAGTTATTTACATTTAAATATGAAGTGTGTGTATCATAAGTATGTGTGTATTTACCATGTAGCTGGA
GGAGTCAAGGAACAGAAAAACCCACCAACCAAACAAGTAAACACACACACCACATCATTCATATGCAAAA
ATCCTTTATTGTTGAGGAATAGAGAGAGACATTATATTTTTAATGTAGGACACTACAGGAACTCTAGGGT
ATTCTACAGTCAATGCCCATTGGGGTTGGGGATATTCACTATGCAGTTTCCACACCAGGGTCAGGTAGAA
AAGATGGAGAAGGAGTCAAAGCACAAGAATGTTACAAGAACAGGGAAGAGAAAGGGTAACAGGAGCGTGC
GCACCTGGGGATTGGGACAGGGAAAGAGGTCTTCCTAATGCAGTCCAGGAGTCCGGAAGTGGAGTACTGA
GGACAAAAAACAAAACAAAACAAAAAAAAACACTGAAATCACATCTTATATCCTGTAAAAAAGTCCCACT
GAAAACCTGCTGTGGCAGCAGGAGAGAAGGGGAGAGGCGTCTGTGAGAGAGGAAAGAACAAGTCTTGACC
CTCAGCCTGCCCTCCAGGCTTTGGGCCTGAGGATGGAACTCTCCTAGCCGCAGGTACTTGCACCTTCCCA
GCTGCCATATTTACATTACTCTACACACCTGTGTGCATGGCCAGTGGGGCCCATGTGGACCAAAGATGTA
GGAACCAAGTTCATGCCATCCTAGCACCACCATGAGCCAGACACACACACAGCCCTGCCCAAGGTGCCCT
ACTATGAATGCTCCCCCTGCCTTATCCAGTTCCTCCTCTTCCCAGAAACCTCCCAGAGGAAGCACAGGCC
CAGGGATTACTTACAGTTTCACATGAATGAAATCAGATGACACAATGATCTGTGTTGATCCATCTCATCT
AATCTTTAAATGCCAGCTCCCAGAAACCTTATGTCCTATTCTGCCTGACACCTAACAGGTTGGGAATGCT
CCCTCAATGGTAAGATTATGAAAGCTGCAATTATGGGTCCCTCTGAAAGAGCCTGGATGCACAAACAAGC
ATTAAAAGTAGATTTCCACAATGCACTTGTTTTTGCCATTTGTATAAAATACTATAGCAAAATTGCACTG
TAATTATCTTTATGGCCAGTGCATAACGCTGGAGCAGAGTATGTCATCCAGATATAGCTGCTCGTATAAT
TATCTGGCCGTCGGCAGGACTGAAATAACCATCTAATGAGAGCAAAATGATACAGTAACTACCATGTAAT
CAGCAAGTTACAGTTATTATAGCTCTTTGTGCCCTCTCACCCAACACTAAATGGGGGGCCAGACTCAGTC
TCTGGTCCCAGGAATTCATGCACAGGCAGCATCATCAAGCACCAGCTCCTTGGGTCTTGGGCGTGCCACC
TTGAGACCAGTGAGTATGGGTAACAGGGACACAGAGAGAAAAGCAGGAGGCTCTCCATGATTACGAGCCT
TCCCAATCTCTCTCCCTCTCCTTTTCACTCCCTCCACCCCAATTCTTTGGAAGCTAGTGAAAAGAAAGCC
ATTGGTGGAGCTGCTCTTGCTGTGTGTGGGAAGACAGTGTCAAGGCGGAAGGTTCGTGTTTTGACTAGAC
ACTTCCCTGGGGGACTCTTACACTCGCATACCAAGTGAGGAATTTGTCAGCCCACTCAGGCCTGGGGTCC
AGACAGAGCCAGTCAGGGGAAACATTAGGACATCCCCCATACCTCCCAAACTGGGACAATTATCATAAAA
TTGGCACACAGCACTGTGCACCCGGCACTGTTCTGAACACTTTACCTATACTCAATCCTCAAAACCACAA
CCCTGTGAGATACTATTATCACTTTCATTTTTCAGATGAGGAAACTGAAGCACAGGAGGATATGTACCTT
GGCCAAAAAGATACAGATAGAGTGGCAGATCCAGGATTCACATTCAGGCCATTGTTCTTATTCATTATGC
TAAAGAGAGATTCAGATATGGTTTCCTGCTAATCCCCATTAGATTTCAGAGTTCCTTGCATTTCTTCCTG
AATCATCCTTGTGTATGACTACCTTCTGGGCCCCGTAACCCAGGATTCTGAGTGATTTGAAAGGCTCAGC
CTGCCTGGGAGGCAAATGCTTGCCTGTGAATTGGTTGTGGTATTTGTAATTATCAGATAATAATTTCTCC
ATCTTTTTGCATAGATTACAAAAGAATGAAGTCCAAACAACAGAAATTGCCCATCGGGGAAGCCCCTCTC
TCCTCATCTCCCAACACTCACTCCTGCTCAATAGTCAGCTGCCGTCCATGATCCAGAGGAAAGAGATGAA
AGACGGGGGATGTGTACAAACACACGTGCCTCTAAGTGTCTTTGTGTTTGGCCGACAGTGTGCTGGGCAT
TGGCAGTGTATTTCAGGGCAAATCTATGAGCTTTAATACCTGTAGCATGGGAGGAAATGCACAGCTGGCA
ACCCCCACAGCCCCCTCCCCAGTCCCCTCTCCCCTCTCTAATGAACAATCCTTACCCTATTCTGCTCTCG
GGGGATACTTGAGGATGCCAAAGCTGGGGACGTTCTCTTGATTCACATCCGTAGGAGAGTCTAGGGTAAA
GCGAACAGTGTAAGTAAGAGGGATCTTTCTGAATGTGAACTACACCCTGATCCAACAGCAAAGGGCTGGC
TATGACGGGCAGTGTTCAAAGACAGAGTTGGGAGACTGGGGTGGAAGCAGA

Protein Sequence : Show Sequence

>gi|6005705|ref|NP_009195.1| G-protein coupled receptor 182 [Homo sapiens]
MSVKPSWGPGPSEGVTAVPTSDLGEIHNWTELLDLFNHTLSECHVELSQSTKRVVLFALYLAMFVVGLVE
NLLVICVNWRGSGRAGLMNLYILNMAIADLGIVLSLPVWMLEVTLDYTWLWGSFSCRFTHYFYFVNMYSS
IFFLVCLSVDRYVTLTSASPSWQRYQHRVRRAMCAGIWVLSAIIPLPEVVHIQLVEGPEPMCLFMAPFET
YSTWALAVALSTTILGFLLPFPLITVFNVLTACRLRQPGQPKSRRHCLLLCAYVAVFVMCWLPYHVTLLL
LTLHGTHISLHCHLVHLLYFFYDVIDCFSMLHCVINPILYNFLSPHFRGRLLNAVVHYLPKDQTKAGTCA
SSSSCSTQHSIIITKGDSQPAAAAPHPEPSLSFQAHHLLPNTSPISPTQPLTPS

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

13. GPX1

Gene Name : GPX1
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 2876
NCBI Protein GI : 41406084
Genbank Accession : AC121247
Protein Accession : NP_000572
Taxonomy ID : 9606
Chromosome No : 3
Gene Starting Position : 49394608
Gene Ending Position : 49395790
Gene Strand (Orientation) : -
Protein Name : transcript variant 1
Protein pI : 6.51
Protein Weight : 20647.83
Protein Length : 203
Protein Note : Also known as GPXD; GSHPX1

DNA Sequence : Show Sequence

>gi|224589815:49394608-49395790 Homo sapiens chromosome 3, GRCh37.p5 Primary Assembly
GTTCTGCTGACACCCGGCACTTTATTAGTGGGGAAACTCGCCTTGGTCTGGCAGAGACTGGGATCAACAG
GACCAGCACCCATCTCGAGGTGGTATTTTCTGTAAGATCAGGTGTTCCTCCCTCGTAGGTTTAGAGGAAA
CACCCTCATAGATGAAAACCCCCCCGAGACAGCAGCACTGCAACTGCCAAGCAGCCGGGGTAGGAGGGGC
GCCCTAGGCACAGCTGGGCCCTTGAGACAGCAGGGCTTCGATGTCAGGCTCGATGTCAATGGTCTGGAAG
CGGCGGCTGTACCTGCGTAGGGGCACACCGTCAGGGCCCACCAGGAACTTCTCAAAGTTCCAGGCAACAT
CGTTGCGACACACCGGAGACCAGGTGATGAGCTTGGGGTCGGTCATAAGCGCGGTGGCGTCGTCGCTGGG
AGCTGGCAGGGCCTCCCGCAGGAAGGCGAAGAGAGGGTGCGCCCCCGCACCGTTCACCTCGCACTTCTCG
AAGAGCATGAAGTTGGGCTCGAACCCACCACCAGGCCGGACGTACTTGAGGGAATTCAGAATCTCTTCGT
TCTTGGCGTTCTCCTACAGGAGAGAAGGGCAGCTAGAACCCGGGGTCAAGAGGAGGAGAGAAACGTTCTA
ACCACAAACAAGGGAGATTTTCTATGAGTCACCGGGATTTTGCCCTCCATGCGCAATCCCAAGGGCGGAG
AGGAATTTCAGCAGCTACGAGCAACAGAAAGGAAACGAGAGAGTAGCCAGACTCTCCGCGCATGGAGCCG
ACGGCACCCACCAGCACACCGCCGGCGCCCCCAGCCACTACTGCACGTCCGCCCCCGCCCCGCCCCGCTC
CGCCCGGCGCACCTGATGCCCAAACTGGTTGCACGGGAAGCCGAGCACCACCAGGCCCCGGGGTCCGAGG
CGCCGCTGCAGCTCGTTCATCTGGGTGTAGTCCCGGACCGTGGTGCCTCAGAGGGACGCCACATTCTCGA
TAAGTAGTACCTTGCCCCGCAGGGAGCCCAGGCTCACAGGCTCCCCGCCGGCCAGCGGGCGCGCCGAGAA
GGCATACACCGACTGGGCCGCCGCCGCCGCCGCCGCTAGCCGAGCAGCACACATGGCGCAATTGTCCAAG
AAGCCAGCGGAGCGCCCCGAACAAGCACTGTAAGGGGAGGCCAGCAGGCGCCTCCTTTTAACT

Protein Sequence : Show Sequence

>gi|41406084|ref|NP_000572.2| glutathione peroxidase 1 isoform 1 [Homo sapiens]
MCAARLAAAAAAAQSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLUGTTVRDYTQMNELQRRLGPRG
LVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDA
TALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

14. HIST1H2BE

Gene Name : HIST1H2BE
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 8344
NCBI Protein GI : 21396484
Genbank Accession : AF531288
Protein Accession : NP_003514
Taxonomy ID : 9606
Chromosome No : 6
Gene Starting Position : 26184023
Gene Ending Position : 26184457
Gene Strand (Orientation) : +
Protein Name : histone cluster 1, H2be
Protein pI : 11
Protein Weight : 13013.18
Protein Length : 126
Protein Note : Also known as H2B.h; H2B/a; H2B/g; H2B/h; H2B/k; H2B/l; H2BFH; H2BFN; dJ221C16.8

DNA Sequence : Show Sequence

>gi|224589818:26184023-26184457 Homo sapiens chromosome 6, GRCh37.p5 Primary Assembly
CATGCCTGAGCCAGCGAAATCCGCTCCCGCCCCGAAGAAGGGCTCCAAGAAGGCCGTGACCAAGGCGCAG
AAGAAGGACGGCAAGAAGCGCAAGCGCAGCCGCAAGGAGAGCTACTCCGTATACGTGTACAAGGTGCTGA
AACAGGTCCACCCCGACACCGGCATCTCCTCTAAAGCCATGGGGATCATGAATTCCTTTGTCAACGACAT
CTTCGAGCGCATCGCCGGCGAGGCTTCCCGCCTGGCGCATTACAACAAGCGCTCGACCATCACCTCCAGG
GAGATCCAGACGGCCGTGCGCCTGCTGCTTCCCGGGGAGCTGGCCAAGCACGCTGTGTCAGAGGGCACCA
AGGCCGTTACCAAGTACACCAGCTCCAAGTAAACTTGTCCCTGCAACTGCCTTAGTAAACCCAAAGGCTC
TTTTCAGAGCCACTC

Protein Sequence : Show Sequence

>gi|21396484|ref|NP_003514.2| histone H2B type 1-C/E/F/G/I [Homo sapiens]
MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDI
FERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

15. HIST2H4A

Gene Name : HIST2H4A
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 8370
NCBI Protein GI : 4504323
Genbank Accession : AF525682
Protein Accession : NP_003539
Taxonomy ID : 9606
Chromosome No : 1
Gene Starting Position : 121215050
Gene Ending Position : 121215445
Gene Strand (Orientation) : -
Protein Name : histone cluster 2, H4a
Protein pI : 11.91
Protein Weight : 10474.58
Protein Length : 103
Protein Note : Also known as H4; H4/n; H4F2; H4FN; FO108; HIST2H4; HIST4H4; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4B

DNA Sequence : Show Sequence

>gi|157704448:121215050-121215445 Homo sapiens chromosome 1, alternate assembly HuRef, whole genome shotgun sequence
GTGGTCGGCCCTAAAAAGGGCCTTTGGGATCGAAACGTGCAAAGCTGGAGCGGCGGCCTAGCCTCCGAAG
CCGTACAGGGTGCGCCCCTGGCGCTTGAGCGCGTACACCACATCCATGGCTGTGACGGTCTTGCGCTTGG
CGTGCTCGGTGTAGGTGACTGCGTCCCGAATCACATTCTCCAGGAACACCTTCAGCACACCGCGGGTCTC
CTCGTAAATGAGGCCAGAGATCCGCTTAACGCCGCCACGCCGAGCTAGACGCCGAATGGCAGGCTTGGTG
ATGCCCTGAATGTTGTCTCTCAAGACCTTGCGGTGGCGCTTAGCGCCCCCTTTGCCTAAGCCTTTTCCGC
CCTTTCCTCTGCCGGACATGACCGCTGGAGCCCGATAGACAGCTTC

Protein Sequence : Show Sequence

>gi|4504323|ref|NP_003539.1| histone H4 [Homo sapiens]
MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDA
VTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

16. human IFNG

Gene Name : human IFNG
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 3458
NCBI Protein GI : 56786138
Genbank Accession : AC007458
Protein Accession : NP_000610
Taxonomy ID : 9606
Chromosome No : 12
Gene Starting Position : 68548549
Gene Ending Position : 68553520
Gene Strand (Orientation) : -
Protein Name : interferon, gamma
Protein pI : 10.01
Protein Weight : 18454.87
Protein Length : 166
Protein Note : Also known as IFG; IFI

DNA Sequence : Show Sequence

>gi|224589803:68548549-68553520 Homo sapiens chromosome 12, GRCh37.p5 Primary Assembly
AGTTGTGAACTTACACTTTATTCATATATATTAGATATTGATAATTTTAACAAATGAGTTACTTTCCATT
TGGGTACAGTCACAGTTGTCAACAATATTTGGAAGCACCAGGCATGAAATCTCCTGAGATGCTATGTTTT
CATCAGGGTCACCTGACACATTCAAGTTCTGTCTGACATGCCATTAAAGCACTGGCTCAGATTGCAGGCA
TATTTTCAAACCGGCAGTAACTGGATAGTATCACTTCACTTATAAGTGTTCATTGTATCATCAAGTGAAA
TAAACACACAACCCATGGGATCTTGCTTAGGTTGGCTGCCTAGTTGGCCCCTGAGATAAAGCCTTGTAAT
CACATAGCCTTGCCTAATTAGTCAGAAAACAAAGGATTAAGTGAGACAGTCACAGGATATAGGAATTATA
AATAATACATATATTAATAGATATTCATTTTCATTACACAAAAGTTGCTATTATAAATACTTATTTGATT
GATGAGTCTAAAAATATATTCCCCATATAAATAATGTTAAATATTAATAAATAGATTTAGATTTAAAATT
CAAATATTGCAGGCAGGACAACCATTACTGGGATGCTCTTCGACCTCGAAACAGCATCTGACTCCTTTTT
CGCTTCCCTGTTTTAGCTGCTGGCGACAGTTCAGCCATCACTTGGATGAGTTCATGTATTGCTTTGCGTT
GGACATTCAAGTCAGTTACCTATCGGGAAAGAAAAGAGCAAAATTAATTTCAGGCATATAAGCCATCAGG
ATATTCTGCTAATGTCATGATGGTCAGTGAAAATAAAAGTATTCCTTTAAAAAATGGACCGTATTACCTT
AATATACAATATTTACTTCTTCAGCAGTTGAAGCTAAAAATTACAATTACATTTCTAGTTGTGCTATAAT
GTGGTGGAGTCCATCACATATCTTTAAATCTACTTCTATAGAATTCTTGAAATTATATAGTGAAAAGACT
CCTGGTTGTGGAGTCAGCATAAAAAGGTGGGTTTGAATCTCATGCATGCAATAATTCTGACCTTGAGCCA
GCTACTTAACCTCTCTGAACTTCTATTTTCTCATCTAAAGGAGGATGAGACTGTTTCTATGACCTCTCTA
TCATCAATCTACCAATTATATCACTTTATACTTCAGTCTTTCCTGTATGCATCAAATGAGTAACTATAGG
TCAATGATTCTCACTGTTGTGCATAGCAGATTAATCCGGAAACCTGTCAAAGACCTCAGAGATCCAGGTG
CATGAAGTGGAAGGACTGTGCCCTGTGCATTCACTAAGCTCCCCAGGTGATTCTGACATCCACCTAAGTT
TGAGAACCACTGATCTAGGTTAGGTACCCTAGAAAACACCAAATCCAAAACGAGTGAAAACTGTAATTTT
TAATTTCTCCCAGATTAGGTCAGTCTCTCAATTTCCATAATAGATTTTAAAATAAACACCCCCAATCATA
TTTATATATGGCTTACTGATATATAAAGTTTAAAATTAAGAATTGCAACAACTTTTCCAGTACCCTGCCT
TCTAGGCAAGCAAATTGAACTACTTGCATCTCCTCACTCTAACCAATAGGGCCATTTAGATGATGCTTCA
TAAAATGTGGACCATTTACCTTCCTCTTGGCTCTGCCATTACATTTTTTCTAATCTTGGGAACACTATGG
CTACTCACCACATTTCTGAGGATGGCTGGGGGATTACAGCTAAAGACTAATTGTCAAAGGGCATAACATG
CATAAAATAGCTTAGCAACTGAGCCAAAGAGAATAATAATTAATAGAGCTCACTCAAGATTGCCCATCAA
GAAACAGGAGGTTTGTAGCTTCCAGAAGGAAGAAACCACAGATTTTTTTTCATTTTTTTAAGGCCCAGTT
CCTGCAGAGTAGAAAAAATTTTCGACAAGCTGTGTGTTTGCAAGTGACAGATTTTACTTTCCAATGTTCA
AATCATCACCAAGAAACTAAGAGACTTATCCAGGACTGAACAGTAAGCCAGGGGCACCACTGGATTAGAA
ACAAGTGCTGAGTGTCTAGTTTATAACCAAAGGAGACCTGTTGCCATGTCTCTCCTTTCTGTTTCTGGGG
GCTTACATGAGAGGAGTGAGAGGCAGGCCCAGCAATAGTGAAAGCTGAGCACATATCCTAAGAACACAAA
TGCTAGCTTGAAGACCCTGGTCCCTCTCATTTGAGCCCATTTATATCAAAGTCAACCCCAAAGGAATCTA
GAGGCCTTTGTCTTCTAGAAAACATTTTGAAAAACATGATTTGTCTTTATGCCTCAACGTTATCTTTCTG
TTTATAGAAGCCATAACTTAAAGATACAAGTTCATGAGGTAAGACTTAGGAGTCTAAGGAGTTCTCCAGC
TGAGATATTCTGAAAGTTGATAGAGAGTTGATAGAACAATCTTTTCATAAGGTATAAATTCTATCTATTT
TTCCCTAAAAACAAACAAACAGCAACCATTCTTGCTTCTAATTGGGCAGTACAATCTGATAGGTTGGCTA
GAGACTTGCAGTGGGGTGTCCCTGGTACCTATTCAAAGACTGTAGCTTTCTTCTATCTCATTCTCATTTT
CTATTCTTTGCATTGTAGAGTTTTGGAGCAAAGAAGGTCATCAAACTTATACAGTGAGCCTAACAGTTTC
CTTTTAAGATGAGGAAACTGAGCCCCAGCCAGCCATGTGATTCATCACAGTTCCTTGGTGGCTGAGTTGG
GAGGAGAACACACATCTTCTCAGCTCCTCCCACTGCTCTTTCCATTAAGACAGACAGCCTCTCATTCAAA
GTAAGAGAATTTCCATCATATGAGCAAGGGACAATGAGAGAACTGCTTCTCAGTACTCCCCGCTTCTTCC
TCACCTACTTCCTCTTCACTGGATTTGTCAACTCACCTGTCTTTACGCAATAGTTACAATGCCAGCATTT
CTCTACATTACATACTTCAGCGATTCTCTTACTGGCTTTGCAAAGTCACCCAAACACGAATGGAAATAGT
AAGGTAGAGTTTCAGCCATGAGTTGAGTTCATAGCTTTAGCAACTGTTAAATAGCTAATGTCTACTTTCT
GGAGAATAAATGCTTTGCAAGACCCTCGGCAATGAAACCAAAGAAAGAATTTAAATAGCCTCACCGAATA
ATTAGTCAGCTTTTCGAAGTCATCTCGTTTCTTTTTGTTGCTATTGAAAAACTTGACATTCATGTCTTCC
TTGATGGTCTCCACACTCTTTTGGATGCTCTGGTCATCTTTAAAGTTTTTAAAAAGTTTGAAGTAAAAGG
AGACAATTTGGCTCTGCATTATTTTTCTGTCACTCTCCTTGGAAGGAAAGAGCACAAACAGAGGATGATG
TGAATTTATCCATCAGAAAGCAAGCAACAGGAAAATTAGCCAAATGGGAATATTCAGCTTACCTCTTTCC
AATTCTTCAAAATGCCTAAGAAAAGAGTTCCATTATCCGCTACATCTGAATGACCTGCATTCTAAAAAAA
AAAAAAGAAAAAATTGGTTTACAATTAGCCCATAAATTGCCTTAAAAATATATTTCAAGTTTCATTGAAC
TCAGATGTGACAATATTCACTGATTTCCTTTTCAACTCTTCTGCTTAGTTCTAACAATAAGTATTCCCAA
AAGGCTTATGTGAGATATAGACAAAGACTATTATGTTCTTTTAGCTTTTTTATTTCCCAATATAACCATT
AAATTGCAATGTCACAAATGAGCTCAACAAAGCTGATACTCCAAAGGTCCCAAAAACTATAGTAAGCTAA
AGAAAGTATTTTCAAGCTAGGCTAAAAAATACAAAAACAAAAAACAGCAAAGCCACCCCACTATAAAATA
CTGCCCCCCAATGGTACAGGTTTCTATTACATCTACTGTGCCTTCCTGTAGGGTATTATTATACGAGCTT
TAAAAGATAGTTCCAAACATGTGCGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATTTGATTTTGTGTT
GTAAGAATAAAATCAATTGTGAATGTCTGAATATTATTATGACAGCTATAATTATAACAGCTAAGTTTTA
AACAAATAATGAAATATTTAAAATGTAAAAACTTTTCATTAACCATCAATAAAATTTTAAAAGAAACTCA
AAAAATTCACTTAAAATTTTTAAACATTCCTTAGCCAGAATCATAATTCTTCCAAAATCCCAGTGAGCAA
CGAAGTTCCGGAATACTTCACAAGTAATATAGATAGAGAAGCAGAATGTTGAAATCACTTTTTGGAGAAA
ATCACCTATCAGTTATTTTTTTAAGCCAAAGCTTTAAGGACCTTTTTGACTTTAATTTGTAAACATTTCC
TATTACCTTTTTAATGCTAATCTTGACCTTAGAGTCTGAGAAGTCAAAAACTAATAACCAATCATTTTTT
TCAATCTGCATACTCCTTGACTATCACAACTGAATGAGTTCCCACCACAAAATTATTAACATCATTATGC
TTCATACCCATATTATGCCCATCTTTTGGAAAATTCATAAATCCCTTTTAAGTCTCTATACAAACTGAGC
AGAAGGTTAAAACAATATCTAGATTATTTAGTCAAGGAACTCATCTAGTCAATAATTAACAAAATAACAC
CAAATCTCAAAATGACTGCCTACAAGAGATGACAGCCTATCAGAGATGCTACAGCAAGTCGATATTCAGT
CATTTTCAACCACAAACAAGTACTATTAAAAAGTCATACTTACAAAATATTTCTTAAGGTTTTCTGCTTC
TTTTACATATGGGTCCTGGCAGTAACAGCCAAGAGAACCCAAAACGATGCAGAGCTGAAAAGCCAAGATA
TAACTTGTATATTTCATCGTTTCCGAGAGAATTAAGCCAAAGAAGTTGAAATCAGTAGTTCTTGTATCAA
GCTGATCAGGTCCAAAGGACTTAACTGATCTTTCTCTTCTAATAGCTGATCTTCAGATGATCAGAACAAT
GT

Protein Sequence : Show Sequence

>gi|56786138|ref|NP_000610.2| interferon gamma precursor [Homo sapiens]
MKYTSYILAFQLCIVLGSLGCYCQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQS
QIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVM
AELSPAAKTGKRKRSQMLFRGRRASQ

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

17. IFNG

Gene Name : IFNG
Sequence Strain (Species/Organism) : Bos taurus
NCBI Gene ID : 281237
NCBI Protein GI : 31982949
Locus Tag : BOS_5228
Genbank Accession : AF140653
Protein Accession : NP_776511
Taxonomy ID : 9913
Chromosome No : 5
Gene Starting Position : 45830157
Gene Ending Position : 45834980
Gene Strand (Orientation) : +
Protein Name : interferon, gamma
Protein pI : 10.24
Protein Weight : 18866.5
Protein Length : 166

DNA Sequence : Show Sequence

>gi|258513362:45830157-45834980 Bos taurus breed Hereford chromosome 5, Bos_taurus_UMD_3.1, whole genome shotgun sequence
AATTAGAAAAGAAAGATCAGCTACCTCCTTGGGACCTGATCATAACACAGGAGCTACCGATTTCAACTAC
TCCGGCCTAACTCTCTCCTAAACAATGAAATATACAAGCTATTTCTTAGCTTTACTGCTCTGTGGGCTTT
TGGGTTTTTCTGGTTCTTATGGCCAGGGCCAATTTTTTAGAGAAATAGAAAACTTAAAGGAGTATTTTGT
AAGTATGATCTTTTAATGAGACTTTCTTGTGATTGAAATGACTTGATGTTTGATGTTGACTTGGAATTCT
ATCTATGATGGGCTCTCATCTCTAGTCTTCAGTCATTTTGAGAAGACTTGCTGTTATTGTGACTGTTAGC
TAGATGTGTTTGTTTGTTTGGTTTTTTTTTTTTTTCTGACTAAACAATCTCTGCTCAGTTTGCTATAGAG
ATTTAGAAGGGATTCATGAATCTTTCAAAAGATGGGCATAATATGTGTATACATCATAGTGTTGATATTT
TGTGATAAGAATCCATTCAACTATGATGTTAAGTAGCAAGCATACTGAAAAGACAGCTGAGTCTTAATTT
TTGATTTCTCATGTTACTATAATGATGACAGTCAGAAGTCAAGATCAGCATTAAAAATGGTCATTTGACA
TAATTGATCAATTAAAGAAGATGAGGTCCTTAAATATTTGGCTTAAAAACAAAAACACTTATAGGTGTTT
TTCTCTAAAAAGTCATTTTAAAATTTTACTATCCCATCTAGATTGTCTGTGAATTACTCTTGGACTCAGT
CACTTGCTGAGATTTTTTTTGTAGGAGTTATAATCTAGTTTATATTTTAAAAAATTTTTTGTCTTGTTTT
AGGAATTTCTTAAAAATTTACTTATGGTTAATTAAAATAGCTTGGGCATTTTAAATATTTTATCATATAT
CCAAATTTAGCTGTTATAATTATAGCTGGAGCTATCTTTTAAAGGCGATATAATACCTTATAGGAGGACA
CAGGTAGAGGTTATGAACCGTATACCATAGGGGGGCAGTATTTTATAGTGAGATGATGTTGTTGATTTTT
TTTTTTTTTTTAACTATACCTTTTTCTTACCATAGTAATTTGGGACAGTTGAAGTATCATCAGTCTTGAA
TGAGTTCATTGTGATGTTATATTAGTAAATAAGAAAGCAAAAATCACATGATAGTCTTTGGCTGTTATCC
AACCAGGTAGCTTTTGTGAATATTTTTTGTTAAAACTAAACAGTGTTGAAAAGAAAATCAGTAAATAGTG
CCAGCATCCAAGTTCAATAAAACTTGAGGTACATTTTTACAGCAATTTATGGACTAATTTTAAGCCAATT
TTTTATTGTGTTGTTTTATTTTCCAGAATGCAAGTAGCCCAGATGTAGCTAAGGGTGGGCCTCTCTTCTC
AGAAATTTTGAAGAATTGGAAAGATGTAAGCTGAACATTTCCATTTGGCTGATTTTCCTGTTGCTTTTTT
TCTGATGGATAAATTCACATCAACCTCTCTTTGTGCTCTTTTCTCCCAAGGAAAGTGACAAAAAAATTAT
TCAGAGCCAAATTGTCTCCTTCTACTTCAAACTCTTTGAAAACCTCAAAGATAACCAGGTCATTCAAAGG
AGCATGGATATCATCAAGCAAGACATGTTTCAGAAGTTCTTGAATGGCAGCTCTGAGAAACTGGAGGACT
TCAAAAAGCTGATTCAAATTCCGGTGAGATGATCTTAATGCTTTCTTTGGTATCATTGCAGAGGCTCTTA
CAAAGCACTTCATTCCCAGAAAATAGAAATTAGATACCAGGTGTAAAGCTCTGAAGTGAACTTGTGGTCA
AAATGCCTCTTTGCTAGTTCTCTTTTGACTTGGGTGACTTTGCCGAGTCAGTTATTGGAGGCACTTAAGT
CTGTGATTTTGGGAAAATACTGGCATTATGCCTATCGCACAAAGATGGGTGAATGGACAAATCAGTGAGG
AGGAAGCAGAGAAGGAGTTGGGGTGATGGGGACAGAGTACTGAAAACCAGTTCTCCCATTGCCCCTTGTC
TGTGTGTTGGAAATTCTCTTGCTTTGAATAGGTTGCATGTCTTAATGGAAAGAGCAGTGGGAGGGAAAGG
GAAGAAGATGTGTGCTCCGCCCAGCTTAGCCACCAAAGAAATGTGTGACTTCAGATGAATCACAGGCCTG
GCTGGGGCTGTTTCCTCATCTTAAAAGGAGCCTGTTGAGTTCACTGTAATTTCTACGGTCTCCTTTGCTC
TAAAATTCTACAATGCCATGGAAAGAAAATGAGAATGAGATAGGAGAAAGGAATAGCCTTTGAGGAGACA
AAGGCCCTCCCACTGTCGAACTCTGGTCAATCACCCAGATTGTATAGCCCAAGGAGAGGTGACTGCGTTT
GTTTTTAGAAAGAAAATAGGTAGAATTGTCCTATCAATGAATTTTTAAAGTTTTTAGAAGCTGAAAGATC
ACTTTGAAGCATAAGGACAAGTCACATTTTCCAAAATTTCACCTAAGGGACAACGGTCTCCAGATTTTTT
TGCGGGGGAGAGTTGGCTTTTGTATAAATAGGCTTGGGTGAGAAGAGGAGGGGTCCCATTATGACTTGTG
CTTTCAGGATTTGTACCAGCTCTTGCTCTAACCTGGTCTGCCTCTCAACAATCTCATATAAGCTCCCAGG
AACTGAAATAAGTGGTTGCTCTTCAAGATTTCTCTAGTCTCCCATATATTAGATGGTATGAGAGTGGCTT
GGAAAAATTATATGTAATGTTTTCCATGTTCAGCAGATCTCCATTGGTTGCAGGCTAGAGAATGAAAAGG
CACTGTTCCTGACCCAGTGCTGCCTCTGGTTGCTGTTCAGTCCTAGAGAAGTCCCTTAGTTTCTTGGTGA
TGCTTAGAAAACTGGAAAGTTATCACTTGTAAACACACAGCTGGTAGAAATTTTTCTACTCTGTTGTAAC
TGGGCCTTGAAAACAATAGGTTGGTCCCATCCTTCTGGAAGCTCCAAGCCTCCTTCTTCACAATGTGCAA
TCTTGAGTGAGTGCTCAACCATTGTCGTCTTTTGCTCAAATGTGAACATATTTAAGGCACGTTTTGCCTT
TTTGTCTTGTAACTTAGAATCTCCTAGTCATTCTCAGAAATGTGGCAAGGTGGCTGCAGTGTTTCAGCTT
TAGAAAGAAATGTAGTGGCAGAGCAAAGATGAAAGCAAATGGTCCTCATTTTATGAAGCATGAATTCACC
TATATTGTCCTACTGGGAAGAGTGAGAAAATAGTTCAATTTGCTGGAGTGGAAGGCAGGGTACCGGGAGA
TTGCTTTCATTTCTAACTTTATTATATAATACCAGTAATTGATTACTACCTTTAAATAAGATCCGTGTTT
ATTTTTAATCCCATCATGGAAACAATGAGACTGACCTAATTTGGAGGAAATTAAAAATAATGGTTTTCCT
CCTTCTAGAACTTGGTGTTTATAAAGTACTGAACCTATACCAATGGTTTTCAAACTTGGGTGGTGCCTAA
ATCACCTGGTGATGTTGGTAACCACACAATGGCCCAGGCCTAGTCCGATTTCACCCATCTGGGTGTCAGA
GTTCTTCATTAGGTCCTCAGGTTACTCTTTTACGCACCAAACATGAGAGCCATTGGCTATAGTAACTCAT
TTGATGCTTACAGCGAAGACTAAGTACTAAGTGATAGCAGTGACGGAGACCCTAGGGATCATCTAAACAC
TCTCCTCATACTTAGTGGAGAAAACAGAGATCTGGAGAGCCTCAGTGACTGGTTTAAAGTCAGATGTATT
GCACACACAGAATTCACACCCACATTTTTCTGTCACTCTACTTCTGGGGTTTTTTCACTATATAATTTTC
AGATCTCTGAAGTGGTAAATTTATGAATATGTGATGTGCTCCATCCTGTTACAGTACAACTATCAATTGA
ATTGTTATAATTTTAGTTTTTATCACTGAAGAAACCAACATTACATATTAAGGTTCATTACTGCTAGTGA
AAATAATTTGTTTTAAAGAATACTTTGATTTTCTTGGGCCTAGACAGCAGAATATCCTAATGACTCATAG
GCCTGAAATTAATTTTGCTGTTTTCTTTCCCAATAGGTGGATGATCTGCAGATCCAGCGCAAAGCCATAA
ATGAACTCATCAAAGTGATGAATGACCTGTCACCAAAATCTAACCTCAGAAAGCGGAAGAGAAGTCAGAA
TCTCTTTCGAGGCCGGAGAGCATCAATGTAATGGTCCTCCTGCCTGCAATATTTGAATTTTTAAATCTAA
ATCTATTTATTAATATTTAATATTTTACATTATTTATATGGGGGATATATATTTAGACATCAAAGTATTT
ATAATAGTAACTTTTATGTCATGAAAATGAGTATCTATTAATATATGTGTTATTTATAATTCCTGTATCC
TGTGACTATTTCACTTGACCCTTTTTTTTCTGAGTAACTAGGCAAGTCTATGGGATTTCAAGGTTTTATC
TCAGGGGCCAACTAGGCAGCTAACCTAAGCAAGAATCTGTGGGTTGTGCACTTATTTCACTTGATGATGT
AATGAATGCTGATAAATGAAATGATGCCATCTAGTCACTACTTATCTGAGACTAGATCTGGATTCTGAGC
CACTACTTTGATGGCATGTCAGACAGCACTTGAATGTGTCAGGCTATATGACTTGTGCCCTGACAAAAAC
ATAGCATCTCATCTCACCTTATACCTGGCGCTTCAGGATATCACTGACAATTGTGATTACACCCAAATGG
AAAGTAATATGTTTGTTTAGTTTATCAATATTTAATATATATGAATAAAGTATAATTTCATAAC

Protein Sequence : Show Sequence

>gi|31982949|ref|NP_776511.1| interferon gamma precursor [Bos taurus]
MKYTSYFLALLLCGLLGFSGSYGQGQFFREIENLKEYFNASSPDVAKGGPLFSEILKNWKDESDKKIIQS
QIVSFYFKLFENLKDNQVIQRSMDIIKQDMFQKFLNGSSEKLEDFKKLIQIPVDDLQIQRKAINELIKVM
NDLSPKSNLRKRKRSQNLFRGRRAST

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

18. Ifng (Interferon gamma)

Gene Name : Ifng (Interferon gamma)
Sequence Strain (Species/Organism) : Mouse
NCBI Gene ID : 15978
NCBI Protein GI : 33468859
Genbank Accession : NM_008337
Protein Accession : NP_032363.1
Other Database IDs : MGI:107656; UniProt: P01580
Taxonomy ID : 10090
Gene Strand (Orientation) : ?

DNA Sequence : Show Sequence

>gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
AGTATACCTTTGCAACC

Protein Sequence : Show Sequence

>gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
LLPESSLRKRKRSRC

Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
Additional Molecule Role : Vaximmutor
Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
Related Vaccine(s): Bacille Calmette-Guérin (BCG) , M. tuberculosis DNA vaccine pAK4-sod , M. tuberculosis HBHA Protein Vaccine , M. tuberculosis phoP mutant SO2 , MVA/IL-15/5Mtb vaccine , Mycobacterium tuberculosis drrC mutant vaccine , Mycobacterium tuberculosis fadD26 mutant vaccine , Mycobacterium tuberculosis mce-2 mutant vaccine , Mycobacterium tuberculosis mce-3 mutant vaccine , Mycobacterium tuberculosis RD1/panCD mutant vaccine , Mycobacterium tuberculosis sigE mutant vaccine

19. IL-1b

Gene Name : IL-1b
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16176
NCBI Protein GI : 6680415
Locus Tag : RP23-384K11.2
Genbank Accession : AL808143
Protein Accession : NP_032387
Taxonomy ID : 10090
Chromosome No : 2
Gene Starting Position : 129364578
Gene Ending Position : 129371119
Gene Strand (Orientation) : -
Protein Name : interleukin 1 beta
Protein pI : 4.31
Protein Weight : 29045.55
Protein Length : 269
Protein Note : Also known as Il-1b; IL-1beta

DNA Sequence : Show Sequence

>gi|372099108:129364578-129371119 Mus musculus strain C57BL/6J chromosome 2, GRCm38 C57BL/6J
GTTTGTTTGTTTTAATGAAATTTATTTCATACTCATCAAAGCAATGTGCTGGTGCTTCATTCATAAAAAG
TCTCATCCATATTATAGGAACCTATTTATTCTCAGCTTCAATGAAAGACCTCAGTGCGGGCTATGACCAA
TTCATCCCCCACACGTTGACAGCTAGGTTCTGTTCTAGAGAGTGCTGCCTAATGTCCCCTTGAATCAACT
TAAATAGATCAACCAATCAATAAATACATAAATAAATAGGTAAGTGGTTGCCCATCAGAGGCAAGGAGGA
AAACACAGGCTCTCTTTGAACAGAATGTGCCATGGTTTCTTGTGACCCTGAGCGACCTGTCTTGGCCGAG
GACTAAGGAGTCCCCTGGAGATTGAGCTGTCTGCTCATTCACGAAAAGGGAGCTCCTTAACATGCCCTGG
GGAAGGCATTAGAAACAGTCCAGCCCATACTTTAGGAAGACACGGATTCCATGGTGAAGTCAATTATGTC
CTGACCACTGTTGTTTCCCAGGAAGACAGGCTTGTGCTCTGCTTGTGAGGTGCTGATGTACCAGTTGGGG
AACTCTGCAGACTCAAACTCCACTTTGCTCTTGACTTCTATCTTGTTGAAGACAAACCGTTTTTCCATCT
TCTTCTTTGGGTATTGCTTGGGATCCACACTCTGCAGAAAAGTAGAAAAAAGAATTGTGTTAACGTCTGT
GTCCGTGTGCATGTTGCATGTATGACAGAAAGGATAAAGCTACCACGGTAGACATATTCTCAGGGCTGCT
GGAGAAGAGAATGAAAAAGAGGTTGTGGAACTTGAGGCCACATTCTTTACTGCTGAACCCCATCTTTCTA
TAATCACTGAAGGAACCTTTGTTATATATATATTTTTTCTGAATCCTACCATATGGGTGAGAATTATATT
AAAGCTCCCAAGGGTACCCAGTTTCCAGGCATTTTTGTCACAGTTCCCCCTAAATCCCTGGATAGAGTGG
CATCAGCTTGGAAATGAAGCTTTTGTTAGGAATGTCACAAGTGCCAGTACAGGTCTGAGGATCGTTGGTT
GAACCCTTTCACTGCATTCTCCAAACTGTTCTCTGTCAAAGGAGGAAAAACTGTCACCATATACAACTCT
TTCATCCTTGATGCTACGACTTCTGTTGGGGGTGATAACGGTGGCCTGACATGACAACAGCATCAAAAAG
GATTGATTTCCCATGGTGGCAGTGAGGTTTATGTGCACTTAAAAGTTATGCTAATTTTATTCTATATTTA
TGTAAATCGTCAAGAAGACCTCTAATGTTTTCTGGCGGGTCAGGCAGGTGGATGTCTGGGAAGTAAGTAA
AGGCAGAGTCTTCGGTGAGCAGTGGGGGAGGCTTCTCTACTGATGGACTCACCTCCAGCTGCAGGGTGGG
TGTGCCGTCTTTCATTACACAGGACAGGTATAGATTCTTTCCTTTGAGGCCCAAGGCCACAGGTATTTTG
TCGTTGCTTGGTTCTCCTTGTACAAAGCTCATGGAGAATATCACTGGAGAAAGGAGAGGAGTCTGGGTCA
AGGGAACAGCAATGCAGGGTGGGCTCAAGCATTAGACGTGACAGGCAAAGCCTATGCAGTCCCCCGTGTG
AACCCAGAATGAGCCTCCTCAGACACAAGATATGGACTAGCAGGGCTATACAGTCATAGTCATAGACAGT
AGCAAGGTCAGTAGGCCGATCCAGGATTCCCTCAGGACCTCTTAGGAGCAGCAATGAAGAACGAAAGGTG
TCTAACTGTTAAGCTCTAAGGGCTAAAGCTGTTATGTGGCTGGCAGGCTGTGTGTCCGTCTACGTGAGAA
GTACAGTTTGGTCGCTATGAAAACAAAGCTGTGCTCCTAAAAGGACCTAGGGGCCAGCACGTTTTGAGGA
AAATAGAGCAGGAGGCAGGTAGAACTCTATTAGCTGGGGAGAGTGGCTCCAAAAAAGAGACCCCCCCCAC
TTTGATCTTAGCCACAGTACCTCAATTGGAGGCCCCTGGCTGCTTTTATGACTTTATCAAGAAATAAGAA
TTATTCTTTTTAAAAGGTATAAAGCAATAAGATTCTTTTCACCTGGACCAGAGTAGACAGCTCCCTCCAA
AGCTTCTAAGATCAGAAGCTAAAAGGAGACAGCACACACTGCTGCTGTTAGCTCTTGTCCCCTCAGCTGT
GGTGGTGTGGTGCCAGGTCCACACCATCATCTAAAGAGGCCCTGACCGCGCTTGGAATGCTTGTTATTAT
GGAAGAGAGATGTATGCTCCTGATAGTCAGCAAACCTCCCTTCAAGGAACTGTGTGTGTGGTATTGGTGG
GAAGGATATCAGCACGAGGGATAGGACTGGGAACAAAAATGGACATATCAATTCTCTACCATCTACATTT
CAGCCTCAAATGAGGGAAAAAGAACCCACAGGCAGTTATTGCATGTCCATCAAATGACTTCACACTAATG
TGTTATCCCTATATGACTGGGCTGGAAAAATGGTCCTTTTTCGTTCTGTCTCTGTCTCTGTCTCTGTCTC
TCTCTCCTTCCTTCCTTCTTTCCTTTCCCTTTCTTTTTCCTTTTCTTTTTTTGTATAATGACCATAGTGT
TCCATTTTAAAACACAGGGACCCAGTAGGCTGCCAGGAGGCCAGAAAGGGAAACCAAGATGCTCCTACTT
ACCTTGTTGGTTGATATTCTGTCCATTGAGGTGGAGAGCTTTCAGCTCATATGGGTCCGACAGCACGAGG
CTTTTTTGTTGTTCATCTCGGAGCCTGTAGTGCAGTTGTCTAATGGGAACGTCACACACCAGCAGGTTAT
CATCATCATCCCATGAGTCACAGAGGATGGGCTCTGAAAGTTCAAGAGCAGATGTTGTTTAGGCAGATAA
TTAGAAATGCAAGCACTGTGAAGTGAAGCAGCAGAATTTGGCTTATTGAGCAACACCTAAGCATGTGCAG
TCAAGATTCTATTCTAATTTTAGTGGGGGTATAAAAACACTGGGAGTGGGCTGGGCAAGAACATTGGATT
TGGCATCGTGAGATAAGCTGCAGTGTTATAACCATTCACTAGGGACTAGCAGTAGTGGTCCTTGCATGAA
ACGATGCCAGTAATAAGACCAGCTTAACTGGGAAATTGTGAGATTCACAACCTCTAGTATAAGCCAAAGT
ACTTGGCAAGTCAATGGTTGAGTTGCAAGTGTAAGCATCATTAAAGCCTTTTTATAAGTTAATCAGCTCA
AGATAGAACTGATTTGTTTGGAGGTTTCCTCATTTTCTTCTAATGGTTGGAATGTCTAGCACTAAGGGTC
TTGCACTTGAGGGGCGTTGAGTTCTAAGAGGTGGGCTGAGAAGGACCAGAGCGAAGGTCTGCTGTCTGCT
CTCAGTCCTCATTAAGTACCTTCTTCAAAGATGAAGGAAAAGAAGGTGCTCATGTCCTCATCCTGGAAGG
TCCACGGGAAAGACACAGGTAGCTGCCACAGCTTCTCCACAGCCACAATGAGTGATACTGCCTGCCTGAA
GCTCTTGTTGATGTGCTGCTGCGAGATTTGAAGCTGGATGCTCTCATCAGGACAGCCCAGGTCAAAGGTT
TGGAAGCAGCCCTGTGGAGAAACCAGGCAGTGAGCACATCAAGTCATCAGACCAGAGGACTCCTACATCA
ACCCCAGAGAACACCAAGGGCCCGGGAGATAAGTTCTTAACCACAATCCCTGTCTATGTGGAGCCCAGAG
AATTTAATTGGCCTACCCAGGGGTACATGGCAACCAGACACAGGCTTCCTGGCATTCTCTATAGTAAACC
TCATTTCTACTTTATCTTTCTTGTACAGACCTTGCCTCACTGTATAATTAGACCTACTTAAACGTTTCAT
CTTTTGGTATCCTTGCTGCTCAAACATCATGTTATTTAATTTCTCATGACAGTATGTTCATGTTGTCTTC
TATTCAACGGAGATCACCCAACAGGCTCTCAATGGCTTCCCATACATTCTTTTTCTCATAGGAATATTTA
GCAGACACTATAAGCATAGCTTTTGTTTGTAAAATATGTCACCACTTTTCTGTTCTTTGGAGAAACACCA
CATTAAGGCCTAAACACAGTTGCCTTGAGGCATGATAACAGTTTCAAAGCTTGAAGGACAGCGATAGATC
ATTTCAATAGTGTAAAGGAAAGGTTGATTGGGAGAGAAGTTTCTGTCCAGAGTAGAGGCTCCCAGGGAAA
GCCATGTTCTGGAGCAGGCAGTGGGGGACAGAGCCTGGCTGGGAAAGTGAGAGGCGTCACCCAAGGCTGA
ACTAACTAGTTAGAGTGGTCTTAAAATACATTCATGTAAGCTAAGGACTAGATTCTGTAGCTGGGGGAGA
CTGTTGAGGGATTGGGAAGAGGTCTGAGAGAAACAGCTGCCTCCTGAGTGGGGCAGTGACGTGGCTGGAC
TGACGCCATGTGGCATTAGAGCGTTCTCAGTCATCATAGGAAGGGACAGCTGGGATGCAACATGGCTCTT
CATGAGAGCAAGTCTGGAAAGTGCCCTTGTGCTAGGATGGACTTGGCCAGCACTTAAAACACTTCAGAAT
GGAAGGTTGTGACAGATCGCCAACATTTCAGAGATCTTCTGACCTGTGTTTCCCAAAGGACACAGTGGGG
ACTGTCTATGATCACACAGCAATGAAAGGTCTTGTCTAAGGCCACAGGTTATTTTTAAAAATCCTAACTA
ACTTTTTGTTTATATCACTTATTTCTTATTATCTTAATCTCCCCAGGAACATGCGAAACTGTTCTTTGGC
AGAGAAGGAAGAGATGCTAATTCTGAAGGTCATGCTCTCTAGATCCAGAGTTCGATTTAAGTGGTCAGAT
TAAGCTCCATCTCTCTCAAGGGGGGACTAAATGTATGTTGAAGACCATACTGTCTTCTTAGCATAAAACA
CCATGATTGCCATCAGCAAGCCTTGGTGTGTGGCTGTGGTAAATGAAACCTGTGTGGGAGCCAGGTCAGT
GTGTGGGTTGCCTTATCTATGTCACTGAAAGAGGCAAAATAATCCCATCACAAAATAGCCTGGGAGCAAG
GCAGTCCCATATCCCTTCCCGTTTGGGTTTTTTTTTCCCATGCCACAGTCCCTCCACAGGTTAGGAGCCA
AGCTCAGAGTCTCACCTTCATCTTTTGGGGTCCGTCAACTTCAAAGAACAGGTCATTCTCATCACTGTAA
TGAAAACAGATGTGGTCAAGTGATAGCTCCTTATCAATACTGAGCAGACAGACTTCCATTGTTCATAAAA
CCACACCTACCATGACTCTTAAAGCTTCCCAAAGACTTCTGGGAAGTTCTACAGTGCTACGTAAGTAACA
GTCTAGAAGCAGATTCTGGGCCAGGTAAGTGGGTGCTCTGGTTGCTCTCTGTTGACTTCTGACACCTAAA
TTCTAGACTAGCAGAGTTAGCTAAAGTCTGAGCTCTCTGCCTTTGCTTCCAAGCCAGACTTTGCAAAAGA
GTCTCTTATCCTGTCCATGTGGAAGTTTTTCTTACCATTGCATCTGTAGGAGTCCCTGTAGTGACAGCAC
CTAAGTCCCTAGGTTGGGCTTGGGAGTGAAGAGGTCAGTCTTTAAGCTAGGTTGCTTGAACTCTGATAGC
CACTGAAGGAGATGAGTTGGGGACTCTCTGGCCTCTGAAAGTAGATAGCCCTCCATTCCTGAAAGCAGAG
TGCTGGATATGTGACCACTCTCCAGTACCCACTGACCTGTCAAAAGGTGGCATTTCACAGTTGAGTTCAG
GAACAGTTGCCATAGCTGCTTCAGACACCTGTGTGAAGAAAGAAAGTGGGTGGGCATCCAGCGTTAGCTC
CCCGAGGGGCGTCCTTCATATGTGTTTCTCTTAGCTCTAGTTCAGGGCAGGCATGCTAAACTGGTCTCTG
AGGAATGAAGGAACGGAGGAGCCGTTGTATAGGTCTAGAACAGTGGCCCTTTTGGAGTCAAGTGGCCCTT
TCACAGGGGTCACCTAAGACCATTGGATAACACAGATATTTACACTTCATAACAGTAGCAAAATTATAGT
TATGAAGTAGCAATGAAAATAATTTTAGGGTGTGTGGGGGGTCACTACAACATGGGGAACAGCATTAGGA
AGATGCTCTGGAAGGAAGCAACAGCAGAGCCAAACCCTAATAAAGAAGCTTTCCTACAAGACAGGAGCTG
CCTTTTAGATGTCAGCGGCTATACAGACAGTACTCATTTCCAGAGTACTTCACAAACCATGGAGGTACAG
CTACTTTTTATAAATAAAAAGCCCAAAGTCCATCAGTGGGGGGGGGGGAAAGAGAGAGAGAGAGAGAGAC
AGAGAGAGACAGAGAGAGAGACAGAGAGACAGAGAGAGACAGACAGAGAGAGACAGAGACAGACAGAGAG
AGAGAGAGATAGACAGACAGAGACAGAGAGAGAGACAGACAGAGACAGAGAGAGAGAGACAGAGAGAGAG
AGATAGAGAGAGACAGAGAGAGAGAGAGACAGAGAGAGAGAGAGACTTACTTGCACAAGGAAGCTTGGCT
GGAGAGGATCCCAGATGAGCCTATTAGGCCTC

Protein Sequence : Show Sequence

>gi|6680415|ref|NP_032387.1| interleukin-1 beta precursor [Mus musculus]
MATVPELNCEMPPFDSDENDLFFEVDGPQKMKGCFQTFDLGCPDESIQLQISQQHINKSFRQAVSLIVAV
EKLWQLPVSFPWTFQDEDMSTFFSFIFEEEPILCDSWDDDDNLLVCDVPIRQLHYRLRDEQQKSLVLSDP
YELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKK
KMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

20. IL-6

Gene Name : IL-6
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16193
NCBI Protein GI : 13624311
Genbank Accession : AC112933
Protein Accession : NP_112445
Taxonomy ID : 10090
Chromosome No : 5
Gene Starting Position : 30013160
Gene Ending Position : 30019967
Gene Strand (Orientation) : +
Protein Name : interleukin 6
Protein pI : 7.5
Protein Weight : 23291.86
Protein Length : 211
Protein Note : Also known as Il-6

DNA Sequence : Show Sequence

>gi|372099105:30013160-30019967 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
ACCAAGAACGATAGTCAATTCCAGAAACCGCTATGAAGTTCCTCTCTGCAAGTAAGTGAAGGCAGTTCCT
TGCCCTCTGGCGGAGCTATTGAGACTGTGAGAGAGGAGTGTGAGGCAGAGAGCCAGCATTGTGGGTTGGC
CAGCAGCCATCAGCTAGCAGCAGGCGCCCAACTGTGCTATCTGCTCACTTGCCGGTTTTCCCTTTTCTCC
ACGCAGGAGACTTCCATCCAGTTGCCTTCTTGGGACTGATGCTGGTGACAACCACGGCCTTCCCTACTTC
ACAAGTCCGGAGAGGAGACTTCACAGAGGATACCACTCCCAACAGACCTGTCTATACCACTTCACAAGTC
GGAGGCTTAATTACACATGTTCTCTGGGAAATCGTGGAAATGAGAAAAGAGGTGGGTAGGCTGTGAAACT
GATGAAGACCCAGTGTGGGCGTCCATTCATTCTCTTTGCTCTTGAATTAGAAATTCTCTGCTGGGATCTA
GGGCCCTTAGGATTTGAAGCTAAAGGTCAGACTAGACTGTGTTCTCTCTTTCTCACCTCTTTGCTGGTTT
TGAGTGGAGGTTGGGAAGGGGTTCCTTTCCTGTCTGGAAGATACAGAATGTGACTGCATTTCTAGAAAAT
TCACAGTGGGCCATTCTCTGTTCCAATTTAGCTATTGCTTCTTAGGTGGGGATTCCTTTTCCCTCATTAT
TCCTTTTTCCTTATCTCTTTGCCTTCATTTATCCCTTGAAGTCTACAAGGACCAGAAAGTGCTTTTTGGC
TGAATGTAGTAAGTTCTGTGTGTGTGAAATCAGGATGCTCTAGGGTCAGCCCAGATAAGATGCATATTGT
ATAGAGCCCAATAAAGTGCTAACACACACACACACACACACACACACACACAACCTAGATTAGGCTGGGC
ATGGTGGTGGATGCCTCCCAGCACTTGGGAGGCACACGCAGGAAGATCTCTGTGAGTTAGAGGCCAGCTT
GGTCTACATATTGAGTTCCAGGATATCCAAGACTACATTGAGAGATCCTTTCTCAAAAAAAAAAAAAAAA
AAAAAAAAACAACAACAACAAACAAACAAACAAACAAGAAAAAAAAAACCCAACCAACCCTACCTAGATT
AAAATATCTCCCATCTTTAAATGAAAAGTATTTTGACTCTGCTTGACAGAAAGGAAAAAAAAAATCACAA
ACAAGATTAATAGCCTAGGTCTGGGTGTGTAGAACCAACTTTGTAATCTTGGGAAAATCCTCAACTATTT
AGTACTCTTTATCTGCATAATTATGGAGTTACAGATAATGGCACAGACCCTTCCAGATGGAATATTCTGT
GACTTAAATTTATTTGCCCCAAGATATCATCAAAATTTCTGTCCTTTACCAAGTTCCTACTCTATGCTGG
GCAGTTTTTATCGTTGAATCCTTGAGACAAATGTATAAGAGGGACATCAATAATTCTTTATTGAAACATA
GGAAATACTTAGGAGAGTAACAAGCTTAAAGCCAAGGGACTGAGCCTAAGGGTGCATTTTTATTTATTGG
GCTGCTTAGTAGGGTTCTAAGAAGGTATAGCTTCCCTTTTCTGTGTCCTGGCTATGAAAGAGCATGACTT
GGCCCTCTAGTGGTGCTTGTTGTAAGAGGTGTTTAAGTGATTGCTCTGGTAATCTTTTGCAGTTGTGCAA
TGGCAATTCTGATTGTATGAACAACGATGATGCACTTGCAGAAAACAATCTGAAACTTCCAGAGATACAA
AGAAATGATGGATGCTACCAAACTGGATATAATCAGGTAGAAACTTGTCACGTTTACTTTCAACAATTCT
TCAAGGTTCTCTCTTGCACGTGATACCTGCATTTGTACAGTCCCAGTCAGGCAACAAAGATGGGGGGATA
CATCCAATACCACTTGGATGTAACTGGCCTGCAGTAGCTTGAATACAAAAGGAGATTGAGAGGTACCTCA
GCAGCTGCTAGGGTGAGGGAAAGTTTACCAACCAGTGCTATAAATGTTCACTCAATCTATGTCCATCAGG
AGCTAAATGTTTAAAGTCTCTATTCTCATCTTGGCTTTGAGGTGTTAGATGATTAAAAAAAAAACATTTT
TTTTGTCTAAAAAAGTGGAAGAGTAGATATAAAACATGTTATGTAAACGTCATGAGGAAAGTAACTTACA
GATTTAATTAAAAAGCAGTTTATTTAAACAGGTAAAGGCCCACTATGCTGGGATTCTTCACCACTGTTAA
ATGTTAGAAGTCATAGCAAGTTTAGCTAGTTCATGAGCCATCTCTTCTCTGGCTGCCCCTGGAAGGATCC
AGGCCTACACTCCCTACTGAGAACACTCTTCACACCCCTCTCCTTCCAGCCGGTGAGATAGTCTACATCA
GGTCTCAGGACAAGTCAGACAAGAACAAGAGTACAGGAAGGGCACTGCAGGATAGAAGGGAAGGAGTGAG
GGTCAGAAGTGGATTCCCACTGCCACACTCTGGGGTAGGGGGTGGGGGGATATTAAGTGGTGCCTCTCTC
TACTTGTCCAGATAATTCAGGAGGTCAGCTGATGCTGCCTGTTGTCTAATTCTGTAGAAGATACTGAACA
GCAGGAATCTTTTTTTCAATTTTTTATTAGATATTTTCTTCATTTACATTTCAAATGCTATCCCCACAGT
CCCCTATACCCTCCCCCCCCTCCCCGCTCCCTTACCCACCAACTCCCACTTCTTGGCCCTGGCATTCCCC
TGTACTGGGGCATATAAAGTTTGCAAGACCAAGGGGCCTCTATTCCCAATGATGGCTGACTAGGCCATCT
TCTGCTACATATGCAGCTAGAGATATGAGCTCTGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTAT
AGGGTTGCAGACCCCTTCAGCTCCTTGGGTACTTTCTCTAGCTCCTCCATTGGGGGCCCTGTGTTCCATC
CAATAGAAGACTGTGAGCATCCACTTTTTGCCAGGCATTGGCCTAGCCTCACACGAGACAGCTATATCAG
GGTCCTTTCAGCAAAATCTTGCTGGTATATGCAATACTGTCTGGGTTTGGTGGCTGATTATGGGATGGAT
CCCTAGGTGGGGTAGTCTCTGGATCTTCCATCCTTTTGTCTTAGCTCCAAACTTTGTCTCTGTAACTCCT
TCCATGGGTATTTTGTTCCCCATTCTAAGGAGGAATGAAGTATCCACATGTTGGTCTTCCTTCTTCTTGA
TTTTCTTGTGTTTTGGAAATTGTACCTTGGGTATTCTAAGTTTCTGGGCTAATATCCACTTATCAGTGAG
TGCATATCAAGTGACTTCTTTTGTGATTGGGTTACCTCACTAAGGATCATACTCTCCAGATACATCCATT
TGACCAAGAATTTCATAAATCCATTGTTTTTAATAGCTGAATAGTACTCCATTTGTAAATGTACCACATT
TTCTGTATCCATTCCTCTGTTGAGGGACATCTGGGTTCTTTCCAGCTTCTGGCTATTATGAATAAGGCTG
CTATGAACATAGTGGAGCATATGTCCTTATTACCAGTTGGAACATCTTCTGCGTATATGCCCAGAAGAGG
TATTGCTGGATCTTCCGGTAGTACTATGTCTAATTTTTTGAGGAACAGCCAGACTGATTTCCAGAGTGGT
TATACAAGCTTGCAATCCCAACAGCAATGGAGGAGTGTTCCTCTTTCTCCACATCCTTACCAGCATCTGC
TGTCACCTGAATTTTTGATCTTAGCCATTCTGACTGGTGTGAGGTGGAATCTCAGGGTTGTTTTGATTTG
CATTTCCCTGATGATTAAGGATGTTGAACATTTTTTCAAGTGCTTCTCAGCCATTCAGTATTCCTCAGTT
GAGAATTCTTTGTTTAGCTTTGTACCCCATTTTTAATGGGGTTATTTGAATTTCTGGAGTTCAGCTTCTT
GAGCTCTTTGTATATATTGGATATTAGTCCCCTATCAGATTTAGGATTGGTAAAAATCCTTTCCCAATCT
GTTGGTGGCCTTTTTGTCTTATTGACAGTGTCTTTTCCCTTACAGAAGCTTTGCAATTTTATGAGGTCCC
ATTTGTCGATTCTCGATTTTACTGTACAAGCCATTGCTGTTCTGTTCAGGAATTTTTCCCCTGTTCCCAT
ATCTACGAGGGTTTTTTTCCCACTTTCTCCTCTATAAATTTCAGTGTCTCTGGTTTTATGTGGAGTTCTT
TGATCCACTTAGACTTGAGCTTTGTACAAGGAGATAAGAATGGATCAATTCGGGCTGGAGAGATGGCTCA
GTGGTTAAGAGCACTGACTGCTCTTTCAGAAGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGTTCAT
AACCATCTATAATAAGATCTGATGTCCTTTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATGTA
ATAAATAAATAAAACTAGATTATTTAAAAAAAAAAAAAAAGAATGGATCAATTCACACTCTTCTACATGA
TAACCACCAGTTGAGCCCGCACCAATTGTTGAAAATGCTCTCTTTTTCCCACTGGATGGTTTTAGCTCCC
TTGTCAAAGATCAAGTGACCATAAGTGTGTGGGTTCATTTCTGTGTCTTCAATTGTGTTCCATTGATCTA
CCTGTCTGTTGCTGTATCAATACCATGCAGTTTTTTTTATCACAATTGCTCTGTAGTACAGCTTGAGGTC
AGGCATGGTGAGTCCACCAGAGGTTCTTTTATTGTTGAGAATAGTTTTTGCTATCCTAGTTTTTTTTTTT
TTTTTTTTTTGAACAGCAGGAATCTTATTCTCAAATTGAATCTATTCCTAGAAAGAACTGACTTCCTTTT
CCATTTTACTTATAGGAAATTTGCCTATTGAAAATTTCCTCTGGTCTTCTGGAGTACCATAGCTACCTGG
AGTACATGAAGAACAACTTAAAAGATAACAAGAAAGACAAAGCCAGAGTCCTTCAGAGAGATACAGAAAC
TCTAATTCATATCTTCAACCAAGAGGTGAGTGCTTCCCCATCTCTCATGCAGTGTGGGAAAGAGGACACC
CGGCACCTCAGGGTAGCGGCACTTTTTCCAGACAGCTGCTCAGAAGGGAGAGAGTCTGAACAACAGTTCT
TGATTATAAGGCCTTGCTTTGTTTTACTTTGGGGTTTTGTTTGAGGTTCTCTTTTGCAAAGAACATCAAT
ACCTGCTTTAAACTGTATTAATAGAATGTTACTAATTGCGTAAGAGGTATGAAAACTATGACAGCCATAC
ATAGTCACCCATTATGAGAGCACAGAGACAAAAGTGACTTTAATATTTAATCCTTGGCAAGTGACATTTT
TGTAACCAGAGTTCTAATGCAGAGAAGTAGCCAAAAGCTAAAATGTCAGGGAAGGAAGGGATCAAGGGCT
TCTGGCTACCATTAGCCAGAAGAATGGTAGATATGGAGGATTAATGACTCAGAGTGTGGGCGAACAAAGC
CAGATGCAATAAGAAGGGCCTGGAATGAAACCCTCTTGCTAAGGCTGCTTTTGCCACTTGTAGTTTCTTG
CCTTAAACCAGAGAGTTTCCCAATTTAATGTGCACAGGAACCACTTAGGGTCTTGTTCCAGCAGGGTCTT
ACTTAGGAGGTCTAGGAAGGGAACTAAGATTCATTTTTGTAAACAGCTGCTTGTTTATGACCCTGCTATA
TAGTATTCAGGCTCCTAAGTAGTGGGGCAAGCCTTCCAGTTAGTCTTCCCCATGCCAGTGGGCCCATGGA
AGGGTGTTTCCAGACTTCTTCATGCTACCACACTAAGAGACTCTCAACAGAGTCTGAATGGAAACCACGA
AGGAACACATTTGTTTTAGATTCCTCTGTGCCACCTTTACTGATGGGAGCTTCTGTTTTCCAGTAGAATA
CAGTATGTTAGCATGGATGCTTGGATAACAGACAAATAAGATGGTGGTGCTGACCTCTGGACGCTTACTC
TCTAGTGGCAGACAGAACAGTAAGGTTAGAATTCTGTTGCTATTAAAAAACTAATAATAATCACCTTGAA
AAAGAATGGAGTTGTTAGGCATGGGTCTCTCTCGAGTAAGCTTGGAACAAAAGCATCTCCCCTGGCTTGG
GTGAGTCAAAGCAGATGGACTTAGCTCGTCTCATTCATTCTAAATTAGAACTTCTTCCCACAGCCCAGAA
CACGCCACAAGAAAAAAAAAATGTGCAATATTTAACCAGTCTTTGTTTTTTCCTCCTTTAGGTAAAAGAT
TTACATAAAATAGTCCTTCCTACCCCAATTTCCAATGCTCTCCTAACAGATAAGCTGGAGTCACAGAAGG
AGTGGCTAAGGACCAAGACCATCCAATTCATCTTGAAATCACTTGAAGAATTTCTAAAAGTCACTTTGAG
ATCTACTCGGCAAACCTAGTGCGTTATGCCTAAGCATATCAGTTTGTGGACATTCCTCACTGTGGTCAGA
AAATATATCCTGTTGTCAGGTATCTGACTTATGTTGTTCTCTACGAAGAACTGACAATATGAATGTTGGG
ACACTATTTTAATTATTTTTAATTTATTGATAATTTAAATAAGTAAACTTTAAGTTAATTTATGATTGAT
ATTTATTATTTTTATGAAGTGTCACTTGAAATGTTATATGTTATAGTTTTGAAATGATAACCTAAAAATC
TATTTGATATAAATATTCTGTTACCTAGCCAGATGGTTTCTTGGAATGTATAAGTTTACCTCAATGAATT
GCTAATTTAAATATGTTTTTAAAGAAATCTTTGTGATGTATTTTTATAATGTTTAGACTGTCTTCAAACA
AATAAATTATATTATATT

Protein Sequence : Show Sequence

>gi|13624311|ref|NP_112445.1| interleukin-6 precursor [Mus musculus]
MKFLSARDFHPVAFLGLMLVTTTAFPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELC
NGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKAR
VLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQ
T

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

21. IL10

Gene Name : IL10
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 3586
NCBI Protein GI : 10835141
Locus Tag : RP11-262N9.1
Genbank Accession : AF295024
Protein Accession : NP_000563
Taxonomy ID : 9606
Chromosome No : 1
Gene Starting Position : 206940947
Gene Ending Position : 206945838
Gene Strand (Orientation) : -
Protein Name : interleukin 10
Protein pI : 8.12
Protein Weight : 19821.37
Protein Length : 178
Protein Note : Also known as CSIF; TGIF; GVHDS; IL-10; IL10A

DNA Sequence : Show Sequence

>gi|224589800:206940947-206945838 Homo sapiens chromosome 1, GRCh37.p5 Primary Assembly
TGATGTGAATAAGATACATTTATTTATTCAAAATTAAAATCTGCTATGAAGACAGACAAACAATGTAACA
TTCCCAGAGGAATTGAATAAATTCTAGTTAAAACTGAGTTCTATTAGAACCAAATTTATTTTTATTTTTA
TTTTTTGAGACAGAGTCTTGCTCTGTCACCCAGGCTGGAGTACAGGGGCATGATATCAGCTCACTGCAAC
TTCCATCTCCTGGGTTCAAGCAATTCTCTTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCGCGCC
ACCATGCCCGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGACCAGGCTGGTTAGGA
ACTCCTGACCTCAAGTGATCCACCCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGC
GCCCGGCCTAGAACCAAATTTAGGTTGTTATAAATAACAAGCTGGCCACAGCTTTCAAGAATGAAGTGGT
TGGGGAATGAGGTTAGGGGAATCCCTCCGAGACACTGGAAGGTGAATTAATCATCAAAGGGGCTCCCTGG
TTTCTCTTCCTAAGAGTATTTGTAGCAGTTAGGAAGCCCCAAGCCCAGAGACAAGATAAATTAGAGGGAG
GTCAGGGAAAACAGCTCAACAGCTAGAAAGCGTGGTCAGGCTTGGAATGGAAGCTTCTGTTGGCTCCCCA
AAGAACATTTTTTTTCCTCCCTTATGTAACTTATAATTTATCTTAAAACACTCAAATACCATAGTGTGTC
ACCCTATGGAAACAGCTTAAAAACAGGTGAAAATAATAAATATTGAAAAAAATTATAATATTGGGCTTCT
TTCTAAATCGTTCACAGAGAAGCTCAGTAAATAAATAGAAATGGGGGTTGAGGTATCAGAGGTAATAAAT
ATTCTATAAGAGAGGTACAATAAGGTTTCTCAAGGGGCTGGGTCAGCTATCCCAGAGCCCCAGATCCGAT
TTTGGAGACCTCTAATTTATGTCCTAGAGTCTATAGAGTCGCCACCCTGATGTCTCAGTTTCGTATCTTC
ATTGTCATGTAGGCTTCTATGTAGTTGATGAAGATGTCAAACTCACTCATGGCTTTGTAGATGCCTTTCT
CTTGGAGCTGTGCAGAGAGATAAGAAACATACAGTTAAAATCCTTTCTGGGGACTAAATAGGCTCCCCTC
CCTCATGCTCATGGATGGGCATGACCTTGAGTGCAGAGGTTGCTTGTTCTCCCTCACGCTGGGAAGTAAA
CCAGAGGATAGGCCTAGCTGCCTTGGGAAAGGCTGTGCATTGACCTTCATCTCCTCCAGGCCTCCCCCTA
GCAAGGGGAGCATTCATTTTGCAAAGCACTGTCTTCAAAATGATGCCTTTTCAACAATTTTAAAGGTCAC
GTATTCACATGATGATTATGCAGAGTTTGATGAAAAGACATTAGAGGAAGCGCTTCGAAAGCAAGACGGT
GAGAGGAGAGGAGGGAGCCTTTTAAATGTCTGTGTTCTCCCACCAACACACTGAGCAGGCCCTGCCCTGC
CGCCCTGACCTACTATGAGAAATTCACACCCCCTGATGTTGGCACTCTGTGCAGTGTCTGTCCCCAGCCC
ACGAGGAGGACATAGGCCGCACGGTTTCTGGGAAATCAGACTGAAAAAGTTCACGCACAGTTGGAAGTGT
GAAACCCCATTTCGTTTGGGAACTGACATTCTCAGGCACCTCCGCAGGTGCGAAGGGCAGATGGAAAGCG
GGCTTGAACCAACTCCAGGTTAAGTGAGAATCCAGCTTCCTGCTCATTCTGAGTTCTGGGGAGTCTGGGC
TGAACCCCCAGGTGTATTTGTATGTGCACATTTATGTGCCAGCTTCCTGCACGTGTGGGTTCAGCCTAGA
TTCCCAACAAGCTACTTTCTGTACTTTCTGTTTCCTTTGTCACTCTCCCCAGCATGCAGCTCACTGTGTT
CACAGGCTGGCCGGCCAGCCTAACCCGCAAGCCTGCAAAGGCAGCGAGCAGTCATTTAGAAACCCCCAAA
GACACTTAACAGAAAACAAATACAATCAAGGCAGCAGCTCCTCACTGGCTGAGCAGGTCATACCATCTGT
CAGGTTCCCACACTCTCTCCAATGAAGAATGGGGCCTATTGAGTCCCCACCACCTTCCATGCTTTGGGGT
TGGGGAGTGGGCATGGGTTGTGCTCTGCAGACCCTCTCTGCCACCATCCAAGCTCACCTTATTAAAGGCA
TTCTTCACCTGCTCCACGGCCTTGCTCTTGTTTTCACAGGGAAGAAATCGATGCTGTGGAAGAAAAGAGA
AAGTGTTGGTGATCCTGGCTTCCAGCTCCATGTCCATCACACTTAGGGGACAAGCTGGTGGCATCATGAG
GAGGCCAGATTTATCCAAATGCCTTGCCTCACAGCTCCCAGAGAGAACTGAGCCCTGCTTCCATGGCCCT
GGGAGTTAAATGTATTTCTCCAGGACTCACTCCAAGATGTTTGCAAGGCTTGGATGACAACCCAGGTCTT
GGGATTCCTTCCTCCCCCAGAACTCTACAGTATCTCCTGCAGTGCTGAGCGAGGCATTCTTCAGCCTGTC
CTTTTCTGCCTCTCTCTCCTGTTCCTCCCCGTCAGTCACAGCCACCGAGGAACACTTCTAAGCATCTGAG
GAGGGAAGCTACTGGGACCAAACCCTTCACATAGCCTTGTCCTACAAATAGACACCAACTGATTTTCCAA
TATTGGTGACAGAACAGGGGAAACCATTAGTAGTGAGGGCATCATTTCAGTTGGAGAAATAAATTACCTG
GAGGAATTACTCATAGACACTAGCCTGGGGATGAATAGCCCCCTTGTCCCTTCTAAAATGCCAGGGGAAA
TCCTGTCAGTCAATTGGGCCCTTCTTAGAGCTTTGCTTTCAAAACATTGAGATTAAAAGAGGTGAATGAT
GTTTCCTCTGCTTGGGAACCTTAGCTCCCCGCCCAGGGTCTAGTTACAAAGAGGCAGTGGAGGCCTGACT
GAAGCTCTGGGCTCCTTTTATGAAGAGAGAGGGGAGTAGTTAATAACCCACAAATGACTCACAAATAACA
GGAAAGCTGTTTGCAAATTTCACATGTAAATGCCTAGCAGCCACCCCCAACGCCTGCTCAAAGAGAAATG
AGCAAGAGATCTGACTCCAGGAGTCTTTCCTCATTTACAGCTAGCTCTGCCAGTCTGTGTCTTTGCTGTG
TCTGTGGATGTGAGTGTCCCTGCTGGTCTGTAGGAGATGGTATTTTGGGGGCAGCTGCAAGGGAAAAAAC
TGATCTGCTACTTACACAGCGCCGTAGCCTCAGCCTGAGGGTCTTCAGGTTCTCCCCCAGGGAGTTCACA
TGCGCCTTGATGTCTGGGTCTTGGTTCTCAGCTTGGGGCATCACCTCCTCCAGGTAAAACTGGATCATCT
CAGACAAGGCTTGGCAACCCAGGTAACCCTAAGGGCAGGAGCCAAAGGTGAGTGAGAGATTGGCGGAGGT
GGCTGGGAGGAGGGGCTGCTGCAACTAGCTTAAGAGGACAGCTTGGTTCTAGCGATCCTCCTTCACCAGA
AGCCTCCCCGAAGGGACTGAGCCCTTTGTAAACCCTCTGGCTGCTGGATGTGCTGAGTTAACATCTTCCC
ACTTCTCCTTTTCAAAGCGAAGGAAACAAACCCAATTCCCTGCAATCAGGAAGCAGAGTCTCCCTTCCCT
TAATCATGCTGCACACTCCCCCAGCACCCCGCCCCTGCTCTCACCTTAAAGTCCTCCAGCAAGGACTCCT
TTAACAACAAGTTGTCCAGCTGATCCTTCATTTGCTGCAGGAAGAACAAAAGGAGAATGAACTTGAGGTT
TGGGGAAATAACTGAAATGCGGTCTTTTTGATGCCCTTTCATTTAGAGATTTTTTTTTTTAAATAAAATT
GGCTCTGGCCCAAAAAAATCAAAAGGGGAGTTTTAAAAACAGAGGATTCAACAGTGATGGGACCATCACT
TAAATCAGGTCCTCCTCCTCAGAGTCAAGTTATTTAAAAAATCTGGCCGATTTTGAAGGGAAAGCAGAAG
CTTGACTCAGTCCTGGTCTTCTTTCTGCGGAGCTACATTCGGCTTTAAGGGACACCTATGTCAACCCTTC
GGGGCCTTGAGCCCCCCTCATGTCCTGTTCTCTGCTTTTCTTTCTCCCCACTGTAGACATCCAGTTTAGC
TTGGAAAGATCCCAGGGATTAAGAAGCTCCTTCTAATGCAGGTTTCCCTCATGTAGAGTGCTTCCCTAAA
CCACTGGTCTTTCAGGTCTGGAAACGCTCTAAGCAGAGGCAATTTAAATTTCCCTGCTGCAAGGCATGGG
GAGCATCTTCACCTCGAATCAGACATTTCTTTACTTCTCCTAGCCAATAAAGTATAGAGCGCCAGCAGGA
TCTTATAAGTTTCTCAGCACGAGAGAGAACGCATTTACTTTATAAGGCAGGTTTCCTGCACATTTACTGT
ATCATCTGGCTTAGCTTAACTCACCAAACCCATGGCTTGATCTAGGATTCTCTTAAGAATTTAAGTTTGG
GGGAATAGGTGTTGGGGATGGAGGTGGAGGCGCAGGAGGAGGGTTCTTATAGTTCCAGGAGAATGTCTAG
TTCAGGCAGTCCCAGGAAGAGAGAAAGGACAGGAAGGAATCATACTCACAAAGAAAGTCTTCACTCTGCT
GAAGGCATCTCGGAGATCTCGAAGCATGTTAGGCAGGTTGCCTGGGAAGTGGGTGCAGCTGTTCTCAGAC
TGGGTGCCCTGGCCTGGGCTGGCCCTCACCCCAGTCAGGAGGACCAGGCAACAGAGCAGTGCTGAGCTGT
GCATGCCTTCTTTTGCAAGTCTGTCTTGTGGTTTGGTTTTGCAAGAGCAAGCCCCTGATGTG

Protein Sequence : Show Sequence

>gi|10835141|ref|NP_000563.1| interleukin-10 precursor [Homo sapiens]
MHSSALLCCLVLLTGVRASPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESL
LEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVE
QVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

22. IL10

Gene Name : IL10
Sequence Strain (Species/Organism) : Bos taurus
NCBI Gene ID : 281246
NCBI Protein GI : 41386772
Locus Tag : BOS_15514
Genbank Accession : GJ062448
Protein Accession : NP_776513
Taxonomy ID : 9913
Chromosome No : 16
Gene Starting Position : 4402473
Gene Ending Position : 4406411
Gene Strand (Orientation) : -
Protein Name : interleukin 10
Protein pI : 8.14
Protein Weight : 19616.38
Protein Length : 178

DNA Sequence : Show Sequence

>gi|258513351:4402473-4406411 Bos taurus breed Hereford chromosome 16, Bos_taurus_UMD_3.1, whole genome shotgun sequence
AAATAGCTCACAGAGAAGCTCAGTAAATAAATATATGTGGGAGCTGAGGTATCAGAGGTAATAAATACTC
TAGGGGAGAGGCACAGTACAGGTCTCCAGGGAGCTGGCTCTGCTCTCCCAGAACCCTGGATTGGATTTCA
GAGGTCTTCCGTTTACGTCATGGAGTCTAGTAGAGTCACCATCCTGGAGGTCTTCTTCCCTAGAATGCTT
CACTTTTGCATCTTCGTTGTCATGTAGGTTTCTATGTAGTTGATGAAGATGTCAAACTCACTCATGGCTT
TGTAGACACCCCTCTCTTGGAGCTGTGTGGGGAGACAAGACGTGTTCAGTGAAAACTCCCTCTGAGCAAG
TGAGTGGGTCCCTGCTGGGAAGGTCATGACCCTGGGCCCAGAGGCTGCACTAGCTTGCTTGCATTGGGAG
ATGAAGGACAGACTCTGGGCATCTGGAGAGATGCCGGACTGTCAGCAAAGGCTATGCGTTGCCCTGGACC
TTGCCCGGTGGGCAGCAAGAGGAACATTCACTTTGCAAAGTGTGGTTTTTCGAACGGTGCTTTTCCAGCA
ATTTTAAACGTCATGTGCTCACGTGACGCTTATTCAATATCTGATGAAAACACATTAGCGGAACCACTTT
GAAAGAAAGACAATGAGAGAGAGAGAGAGAGGTAGCCTTGTTCCTGTTTATGTTCCCCCACCCGTGGAGT
GAGCAGGCCCAGCCCCGCTGCTCTGACCTACTGCAGGGAGGTTCATGGCCCCTGATACTGGAACCTGCCC
AGGGTCTGTCCCCCACCCACGAGGAGGACATAGGTTCCGCGGCTTCTGGAAAATGGGGTAGAAGAGCTCA
TGGACAGCTGGAAGTGAGAAAGGCCACTTCGTTTGAGAGCTGAGATTCTCAGGTGCCTTGTGTGGTGAGG
CTGGCGGGTGGAAAGAGAGGGGTCAAGTCAGGTAAGGAGAGCTCTGCTTCCTGCTCTGGGCTCGGGGTGC
TCAGGCTGCGCCCCTGGGTGTGTTTGTAAGTGCACGTTCACGTGTCAGCTTCCTGCACGTGCGGCTTCAG
ACTGGATCCCAACAAGCTACTTTCTGTTTCCTCTGTCACTCTCCCCAGCACCCCGCTCGCTGTGTTCACA
GCTGGCGGGCCAGCCCAACCGGCAGGCCTGCGAAGGCAGGGAGCAGTCATTTAGAAACCCCCAAAGACAC
TTCACAGAAAACAAATATAACTGAGGTGGCGGCCCCTCACTGGATGAAGAGGTCACCCCACTTGCCGGGT
TCCCACACTCCCTCCGAGCAAAGTTCAGGGCCTGCTGAGCACCTGCCCACCTCTGCCCCTTCGAGAGTGG
ATGGAGGTCACGGTCCTCAGGCCCTCCCTGCCAGCAGCCCAGCCTCACCTCACTGAAGACTCTCTTCACC
TTCTCCACCGCCTTGCTCTTGTTTTCGCAGGGCAGAAAGCGATGCTGTGGAAAAGGAGAGAGTATGAGCA
ACATTGGTGGCTGGTCCCAGATGCACCGTGCTTGGGAGACAGCCTGGGAGCAGGAGGAGGCCAGCTCATC
TTGAAGGCCCACCCTGCATCTCAGGGAGGACTGAGCCTGGGCACCCTTCCCCACCAGGCAGAGAGGAAGC
ACAGCAAGGCAGGGGCCAGGCGGCATGTGTGTCTCTAGGAGCTCCTCCCCACCGTTAACAAGGCTGCATC
AACACCTCTGTCTTGGAGTTTCCTTCCGGGCGCATCCCAGTGCCAACCAGACTGCTGAGCCAGCCCTTCT
TCCATCCTGTCCTGCTTCTGGCCTGTCCCCCTCCTTGGTCCCAGCCACAAGGACCATTTCTAAGTGCCCC
GCAGAGCAGAGACACAGGGTCAGCCCTGCCAGCCAACCCCCTTGCCTAGCGCTGTCCTGCAAATACAGCC
CAACTGATTTCTCAACATCGGCGAGGGAGCAGAGGAAACTGGTGGGGAGGGTATCATTGCAGTTGGAGGG
ATCAGTAATCCTGGGGGATTATTCGCAGACACTAGCCTGGGACCAAGGATCCCCCTGGCTCTTCTAAAAT
GCCAGGACAGTCCTGGCAGTCAGCCGGGCCCCACCTGAAGCTCTGCTTTCAAAAGGTCGAGAAGAAGAGG
AAGGGGCCTTCCCTCTCCCAGGCCCTCTGCTTCTGCCTGCTTCCGTGGGGCCCAGGGCCTCTGTCCCCAG
GGACAAGGGAGCCCTGGCTGAAGCTCTGGGCCCCTTTCACGTCGAGGCCGGGGACTGCTAATAACCCACA
AATGACTCACAAATAACAGGAAAGCTATTGCAAATCTTCACATGTAAGCACTCAGCAACCACCCCCACCC
GCGGCTCAGAAACAGCCGAGCCAGGCCTGGGGTTCCGGAGGGGCAGGGGCCCCGAGGGCTGGTGTCCCGG
GGTCTCTGGGCGAGAGCAGAGCTTGAAGAGGAGGTCTCAGCACGAGCCCAGAGGAGCCAGAGCTGGCCTG
CTACTCACACAGCGCCGCAGCCGCAGCCGGAGGGTCTTCAGCTTCTCCCCCAGTGAGTTCACGTGTTCCT
TGATGTCAGGCCCGTGGTTCTCAGCCTGTGGCATCACCTCTTCCAGGTAAAACTGGATCATTTCCGACAA
GGCTTGGCAACCCAGGTAACCCTGAGGGCGGAAACCAAGGCGGGTGGTGACCCGGGAGGAAGGGCTACAC
TGCCTGAGAGGACGGCTCTGTTCCCCAAGGACCTTGTTCAGTGGCCACCTCCCCCTGGAACGGAGCCCTT
GGCAACACCCAACACCCTGCCTCCCTCTGGCTATGTGCTGAGTTAAGATCTTCCCACTTCTCCTTTTTTT
GAAGTGAAGGAAGCAGACCCAGCTCCCTGCAGGCGAAGAAGCAGAGTCTCCCTTGGGCACACCGCAAGCT
CCTTGCCCCCGCCAAACGCACTCACCCCAGCCCTCACCTTAAAGTCATCCAGCAGAGACTGGGTCAACAG
TAAGCTGTGCAGTTGGTCCTTCATTTGCTGCAAGAAGAACAGAAGGAGAATGAACCCAAGGTTTGGAGCA
ATACTGAGAAGGACTGTCTTTCTGATATCCTTTTATTGAGAGACTTTTTGATTTTTAGTCAACACAGCCC
TGTCTCCAAAAGGGAAAGGAGAGGTTTTGAAACAGGACATTCAGCAGCGCTGAAGCCCTTAGTTAAACCA
GGTCCTTCCTCCGGGGATCAAGTTCTTTTAAAAACCTGGCTGATTTTGAAGGCAAGGCAGAAGCCTGCCT
GGGTCCCAGTCTTCTTTCTGTAGAGCTGCGTCACGTGTGCAGGGACCCCCATTCCCAACCCCTCAGCACC
CTCTGCTTTGCTGTCTCCAGCTGCAGCACTGACCGTTCTCTGGGAGTGGCAAGCATCTTCTCCTGCAGGT
CTCCCCCTGTATGCGCTACCTCCAACCCATTTGTCATTCAGGTTCTGGAAGCCCTCCAAGCTGAGGCAAC
TTTTGTATCCCTGCCACGGGGTTTTGGGAACATCTTCACCTCCAATCAGATCTCTCCCAGCCAATGAGGT
ATTGAGCTCCAGAGGAATCCTTTACAGATTTTCTAGCATAGGCTAGATGTGCCTTTACATTTTTAAAGAC
AACTTTTCTTGCACAATCTGCTACATCACCAGAGCTCAGATTAACTCACTGAACCCACAGTTTGACCAGG
GACTCTTAAGAATTTCAGCTTCGAGGGGAATGGGTGCTGAGGGTGGAGGTGAGTGAAAAGGAGGCTCTTG
TGAGTGCCTGCAGAATGGCTCTTCCTGCGGTCCTAAGGGAGAAATGAAGGACAGGAGGGCTTTATACTCA
CAAAGAAAGTCTTCACCTTGCCGAAGGCAGCTCGGAGCTCCCGCAGCATGTGGGGCAGGCTGGTTGGCAA
GTGGATACAGCTGCTGTCAGACAGGGTGCTCGCATCTCGGCTGGCTGCCACCCCAGCCAGGAAGACCAGG
CAACAGAGCAGGGCTGAGC

Protein Sequence : Show Sequence

>gi|41386772|ref|NP_776513.1| interleukin-10 precursor [Bos taurus]
MHSSALLCCLVFLAGVAASRDASTLSDSSCIHLPTSLPHMLRELRAAFGEAKTFFQMKDQLHSLLLTQSL
LDDFKGYLGCQALSEMIQFYLEEVMPQAENHGPDIKEHVNSLGEKLKTLRLRLRRCHRFLPCENKSKAVE
KVKRVFSELQERGVYKAMSEFDIFINYIETYMTTKMQK

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

23. Il10 (interleukin 10)

Gene Name : Il10 (interleukin 10)
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16153
NCBI Protein GI : 6754318
Locus Tag : AL513351.1
Genbank Accession : NM_010548
Protein Accession : NM_010548
Other Database IDs : MGI:96537; Ensembl:ENSMUSG00000016529;
Taxonomy ID : 10090
Gene Strand (Orientation) : ?

DNA Sequence : Show Sequence

>gi|6754317|ref|NM_010548.1| Mus musculus interleukin 10 (Il10), mRNA
GGGGGGGGGGATTTAGAGACTTGCTCTTGCACTACCAAAGCCACAAAGCAGCCTTGCAGAAAAGAGAGCT
CCATCATGCCTGGCTCAGCACTGCTATGCTGCCTGCTCTTACTGACTGGCATGAGGATCAGCAGGGGCCA
GTACAGCCGGGAAGACAATAACTGCACCCACTTCCCAGTCGGCCAGAGCCACATGCTCCTAGAGCTGCGG
ACTGCCTTCAGCCAGGTGAAGACTTTCTTTCAAACAAAGGACCAGCTGGACAACATACTGCTAACCGACT
CCTTAATGCAGGACTTTAAGGGTTACTTGGGTTGCCAAGCCTTATCGGAAATGATCCAGTTTTACCTGGT
AGAAGTGATGCCCCAGGCAGAGAAGCATGGCCCAGAAATCAAGGAGCATTTGAATTCCCTGGGTGAGAAG
CTGAAGACCCTCAGGATGCGGCTGAGGCGCTGTCATCGATTTCTCCCCTGTGAAAATAAGAGCAAGGCAG
TGGAGCAGGTGAAGAGTGATTTTAATAAGCTCCAAGACCAAGGTGTCTACAAGGCCATGAATGAATTTGA
CATCTTCATCAACTGCATAGAAGCATACATGATGATCAAAATGAAAAGCTAAAACACCTGCAGTGTGTAT
TGAGTCTGCTGGACTCCAGGACCTAGACAGAGCTCTCTAAATCTGATCCAGGGATCTTAGCTAACGGAAA
CAACTCCTTGGAAAACCTCGTTTGTACCTCTCTCCGAAATATTTATTACCTCTGATACCTCAGTTCCCAT
TCTATTTATTCACTGAGCTTCTCTGTGAACTATTTAGAAAGAAGCCCAATATTATAATTTTACAGTATTT
ATTATTTTTAACCTGTGTTTAAGCTGTTTCCATTGGGGACACTTTATAGTATTTAAAGGGAGATTATATT
ATATGATGGGAGGGGTTCTTCCTTGGGAAGCAATTGAAGCTTCTATTCTAAGGCTGGCCACACTTGAGAG
CTGCAGGGCCCTTTGCTATGGTGTCCTTTCAATTGCTCTCATCCCTGAGTTCAGAGCTCCTAAGAGAGTT
GTGAAGAAACTCATGGGTCTTGGGAAGAGAAACCAGGGAGATCCTTTGATGATCATTCCTGCAGCAGCTC
AGAGGGTTCCCCTACTGTCATCCCCCAGCCGCTTCATCCCTGAAAACTGTGGCCAGTTTGTTATTTATAA
CCACCTAAAATTAGTTCTAATAGAACTCATTTTTAACTAGAAGTAATGCAATTCCTCTGGGAATGGTGTA
TTGTTTGTCTGCCTTTGTAGCAGCATCTAATTTTGAATAAATGGATCTTATTCG

Protein Sequence : Show Sequence

>gi|6754318|ref|NP_034678.1| interleukin 10 [Mus musculus]
MPGSALLCCLLLLTGMRISRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSL
MQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVE
QVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS

Molecule Role Annotation : IL-10 plays an important role in Th2 type immune response.
Related Vaccine(s): M. tuberculosis DNA vaccine pAK4-sod , Mycobacterium tuberculosis sigE mutant vaccine

24. Il12b

Gene Name : Il12b
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16160
NCBI Protein GI : 6680397
Locus Tag : RP23-388G23.1
Genbank Accession : AL607030
Protein Accession : NP_032378
Taxonomy ID : 10090
Chromosome No : 11
Gene Starting Position : 44400062
Gene Ending Position : 44414016
Gene Strand (Orientation) : +
Protein Name : interleukin 12b
Protein pI : 6.5
Protein Weight : 35952.67
Protein Length : 335
Protein Note : Also known as p40; Il-12b; Il12p40; Il-12p40

DNA Sequence : Show Sequence

>gi|372099099:44400062-44414016 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
CAGAAGGAACAGTGGGTGTCCAGGCACATCAGACCAGGCAGCTCGCAGCAAAGCAAGGTAAGTTCTCTCC
TCTTCCCTGTCGCTAACTCCCTGCATCTAGAGGCTGTCCAGATTCAGACTCCAGGGGACAGGCTACCCCT
GAACCAGGCAGCGTGGGAGTGGGGTAAGTGGATTCTGGGAGCATCTCGGATGGCTTTCCCCGCTGGTGGA
AACCAGGGGCTTTGACGACAGGGGCTTCAAATAGTCATTTAAAAAGTCAGCTAATTACAGATGTGATGGC
GCACACCTGTAATCTCCAAATCTGGGAGGCAGGAAACAGAATGCACAGGAGTTTAAGGCCAGATTCAGCT
GTGTAATGAGTTTGAGAGAGTCTCAAAAAATTGAGCTACTAATAATATGTGCCAAGCATTGTGGACAGAT
GTGTATTTGATAGAAAGGTTTGCTTTGTCTATATAGACACATATACTATGTGCATTTTCTTAAGTATTAA
TGCATATGTGTTAAGGAGTGTTGTTAAAATTCTGTTCTTGCCTTAAGAGTGAAACTTAAAAAAAAAAAAA
GCCTTTCATGTACCCCTTCAAGCCTAAGCTCATCACTGAATGTCCTACCTGTGATGGCCTTTAGAATCAG
CTATGATAATCAAAGTGGAGAGCTGGGTTTGGGGGTTCCTTTGGATTTTAACTAACTTTGCCACCTCTTC
AAAACTGGAAAGCCCAAGGCATGTTCCAAACTATTTCCTAAAGGGTTCTAAAATCTGTGAGAAAGGGAAG
GCTAATCTTCATGGGTTGGCTTCCTCTGCTTGCCCAGGTGAAATCATGAGCACCAGATGGGGCAGGGTGA
TGGGGCAGGGTGACATCTTGCTTTCACCCAGGGATCACTGAGCCTCAGCTGGGGGCTCCGTTTGTTCAAG
CAAGGCATTTCAAGATGAAGTTAGGTCACCCCCAAGAATTCATTTTGCTGAGATCTGAAATACTGTGATT
ATTGCACCAACAATCCATGGACTCTCTCTAGGCTAGATTCTGGATATAAGACTAGACAGATATGACCTCT
GTCACACACCTCGGTATGGGAAAGGGGGGACAGACATTAAAGACGTAATCAAATGATTGTCATTTCAGCG
ATGCTGAAGGGGAAGAGTAGCTATTTGGGGTCCTGTTTTCATTTGGGGCTCAAGCTGGCACTTTCTGTGG
ATCATTTCATGAACTCCTAACCTGACAGAGTTATTTTTAATCTAGGAGTACAGGCTTGACAAAGTCAACA
CAACTTGTCAAGGTCACATAACTAACAAGTGGCAGACTGAGGTCTGTTTGGCTGTAGAACCAAGGCCCTT
TGCCCGTGCCCTCCTTGCCATCCCTGACAGCCACGCCATCTGTACTGTGCATTGTTGTTTCTGGGCATTT
TTATTTTTACATATCATTTCTCTTTTAAATTAAAGAATAACAGAATGAAACTTGCAAAAGGGATGTCCAC
TCAGGCACCCGCTGGCAGCAATGTTTGGAAGCATTTGCATGCTCTAGGGAGCTAGAGTATCCAGCCCAGG
GTTCCTGTGCTCTTTGCACAAGACTTTCAGGACTTAAGAAAACTAATGAAAACAAACAAACAAACAAACA
AACAAAAAAGAATTGTTAAAACATAGCATATGGAAGAATTGTGGAGTGAGAGGGAAACAAAGCCCAGCAT
CTGCCAATCAGCCTGAAAGCCAAAGCCTGGCTACATACTGCACACTTTTTTGCTTGTGCAGATTTGATTA
AGAGGCGACCTGCCATCATCTCCACCCTCTGCTGGTCTTCTATGATCTAAGATGTAGGTGATGCCGAAAT
GAAGATTGTTCCTTGGACCATTGCCAGGGCTCCCTTCAGCCTGAAATATGGTTCACGTGTGATGCTCAAG
TGCATCTGAGGCTGACAGAGGAAATGCTTGGCATGTAGCCAAAGGGGAAATCCCTGTGTTGAGAGCATAT
GAAGAGATCACCAGGAGATGCCAGGAATCTCTGCCTTTTTTCCCTGCCCAGAGTCCTACCATCTGGGTTT
CACAGCCGTGTATAATTGCATCACCTTCTCTGGGCATTTCTCTCTACAGCCTGGCAGATTTTTATTTTTC
AACCCAGTGTGCACAAAACAGAAAGAATTTCAATTTTTTTCCTTAGCTTTTTACTAGTGCAAGGTATTAT
ACAATGTCATCTCACTGCATGCCCAATAGCTCTTGAAGATCACATAAGCCTTATGTCTTTTTATATGAAG
GCAGTCGGGCTCAGAGGAACTAAACCAACACCAAACAGCTACTGAGTAGTAGGGCCAGAATCCAAGTCCA
TGTCTGCTTGACTCCAAGCCTATGAATGTAGCAACCAGCACCATTTCAGCTTGTACTTGTCTTGACTATA
TGATAATTTGGACCTAGACATGTCCTGACTCCTAGTCCTGTCTCTGTCATGAAAAAGAAATCATAGCAGG
GCCCTCAAGAGGTTAGCAGTACTTCTGTTCTCTCAAAGGTCGTGATTTCTGACCAAGGCCTGTTTATTTT
TAAGGCATTTGCCTATAACATTTAAGCCTTAATTCATATGGGTTAGACTGTGGTCTCTCAAATCTTGTTC
CTGACGGGCGGTGGCACCCTCCAGGATAGATGAAAAGTATTTCTGAAGGGAATTAGTAATCTACACTGAT
TATCTCAACCACATTTATGAGTGAGGTATAATCTTTACAGTCTAAAGGTTTGTGTTGCCCTGGTGTTAAT
CTAGACTGGGTTACAATACAGGATGGTTACTGACTTATCTATTTCATGATAGGTTAATCAATATGGTACA
GTCTTCTGCCAAAGAACACCTTGAATTTTAGGAGTTCTGGACTCTCAAACCTAGCTAGCACATTTGCCCT
CACAATGTGTTCAATGGATCATAACATCAGCTTTCAGCACCTCCAAGACATGTTAAGTCAGACACTCTGT
TCTGGGCTGAGACCCAACAGTCTCCGTGAACCCAGGTTATACACACACCCAAGCTTAAGAAATGCTGCAT
TTTGTTGTGAGGGAGAGAATTATTTTAGAATTTGGAGTAATCTCTGCCTAGGTTTAAAAAAACCTAATCC
ACTACAGGTCCTTGTCAGAACTTGAAAGTTTACCCTGTGTCACCTTTCCCACACCTAAGCTGGTCCTCTA
GCCTGGGGCATCCCAGTGCCTACTGCACAGCAGGGTGATTCGGATGCCCAGTGACACTGTTCTCGTCCAT
ACTGGCAGCATGGCAGTACTTTCCCATTCTGTTCAAAGCTCTTTGGGTATTATGTTAATATTACAATCAT
AACTAAGGAAATATCTGATGACTGTGGACCTGGGAAGCCACGGTCATCTCTAAGTTTATGTTTCTGAACC
TTAAAAAAAAATAAATAAGATGGGTGTGGTGGCTCTCGCCTTTAATCCCAGCACTCAGGAAGCAGAGGCA
GGTGGATCCCTGTGAGTTTGGGGTCAGCCTTATCTACATAGAGTTCCAGGACAACGAAGGCTGAAAGTCT
GTCTTAAGAAGAGGGAGGGAGGAGATGATTGTGGATATCAGGAAGACAGTTCAGCAGGTCACATTACTTC
TGCACAAGCCTGGTGACCTGGGTTTGAACCCTGAACCCAGAGTGAAAGGAGACAGTCAACTCCCAAAAGT
TGTCCTCTGACCTCCACACATGCACACATACAATAGTCATACATCAATATATTTTTAAAAAACAAAATGG
TATCAGGTAGCTGGGCCCTATGATTATTTAAGCTCCAGGTAGCCTCTAGTTCTTTATTTGGAGATGTTTT
TACCCAAATCTAGAAGAGACCTCCTATAATCCTGGTCCAACAGTGTGAAAAGAAAGGGGAAAGTGAGTAT
TTGGAAGGCAGATAAGCTGTTGATCGGGTTAATTGGATAACAGTTGACATTCTCTCTCCCATTTCCCCGC
CTTTTCCTTCTCCTCTGCTCCCCTTCTTCCTCCCTCCTGCCCCTCCTCCACACAGATGTGTCCTCAGAAG
CTAACCATCTCCTGGTTTGCCATCGTTTTGCTGGTGTCTCCACTCATGGCCATGTGGGAGCTGGAGAAAG
ACGGTAAGCAGCCTTTTCCCTGTTTTCTTTGGAGCGCCAGCACCTAAACTTGATCTCCAGGCAGCCTGCT
GGGAAGGGATAGTGAAGTCTGAAGTAGGCAACCGTGCATGGGATTGTCTCAGGAAAAATCCTCAGGGAAG
ATGGAGGGTTAGCGTCTCTTCCTCCACCTATCTCTCTCCCTTGTTTGGTTTTCATGTTGGATGACAGGAA
AGGAGTTTCAGGTGAACTATGGTGGTGTGTGGGATATGATCTTGGCCTTAGATACCAGAAAATGACTCTT
CTAAACAATTGAAATTCCCTAATAGAGAATTTGTAACGTAGCAAGATTGTCTTCTACCCTCTCTTGTCTT
GCATATTACAACGTGCTCTCAGTGGGCACCATGATGGTTGTCCTCTGGCTATTTTGGGATTGGCATTCAC
ACTGACTTGGTTTCAGGTCATGGCTCTGCCAGGTATTGATACTGAACCTTTGAGGCTGGGTAGTCTTGTG
ACCTCAGTTTTCTTTCCTTTTTTTTTCCCCCAGACAGGGTTTCTCTATATAGCCTTGACTTGTCCTGAAA
CTCACTCTGTAGACCAGGCTGGCCTTAAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGAGATT
AAAGGAATGCACCACCACTGCCTGGCTGACCTCAGTTTTCTTAATTGGGTGTTCAGTATAGCACAGCCCA
CATGGACTATTGAATATGTTAATAAATACAGAAAATAACATATGGTAGAGTGAGCAAGCACTATTATTTA
AAACAAAAAGTCTCTGAACTTTTCATTATCCCAATGGCAATAAGATTATTAGTAGACAAATAATGAAGGC
ATAGAAGGGGGTGCTCTCTGTCCTCTGTCCTTGTGCCATGTCACAGTTAGCAAATGTCCATCAACTCAGT
CCTCAGATGGTCGTCACAGGATCTCAAATGTTTCATGGAATACAAGGTAATCAAGTCAGGAATTTCTGCT
TTTATAGCTACACTTTAGTAATAATTAATATTTATTTAACACTCAATTTCGCAACAACCATGTAAAGTAG
GAGCTATCTGTATTCCATTTTACAGATAGTAAAAACTGAGGTTCAAGAGGCTAAGTAAGTTTCCCACAAG
GTCCAGAATAGAACTTGATCCCAGTTTAGTCTCACTTCAGAGCCTGTGTGCTTAAATGCTAGATTTTACA
AGTAAGGCGTGAAGCAACTATTTCCTGTGGCCATAAAGAGGAAATGGGTGTAAATTATAAATGAAAAGAT
TTCCCAGCTGCTGATTAGAAATAATCTTCTGAGAATCCAGGAAAAAAAAAAAAGGTGAGCCTTTTTTTCA
TTCTGAAGAATTACAAGAGTTTGAACGATATTCGCAGGTAAAGGTGCTTGCCACCAAGTCCGATGACCTA
GACTTCATCACTGCGATCAGCATGATGGAAGGAGAGGATCGAGTTCTCTAAATTGCCCTCTGCTCTCAAG
ACATGAGCTGTGACACCTGTGCACACACACACACACACACACACACATGAAAGAATTAAATGCAACTTTA
AAAAGAGTAATCTCCAGCCTGGCCACCCACGCCAACAGAATTTTGGATGCTTTCCTAAGTAAAGCCTTTG
CCACAGCCAGATGGACAACTTAGGCCCTGGCAGAGTTAACCCCTTAGTTACTTCTGACCTGATAAACCAC
AGAAATTTCAGAAAAGCAACAGGAGAAACACAGAGAGAGCTTGTGTTGGAGTCACAATGGTGGCCCGAAG
CCCAGCAGATATCTAAGCTCTGATTCCAACGTGTCCAAGAAGTTCTTTCCCTGCCCTGGGGCTAAGGTCT
TCTAGGGTTTCTGAGTTCAGGTTCTAAAAGGAAAATAATAGACAGCCTGTTTCATGGCCTAGGGGACAAA
GGGAAACTGGGAGCCAAAGGGTCCTGTGCACTGGAATCAGCTCAGGCTAACGATTTCTGAAGAGCTTGGC
CTCAAGTGATTTGTACCTCTTATCTTTGCTCCCCATTCAGCAGTGACAAATCCCACTGATGTAAAAGTAA
GAGCTTAGCATTTCACAAGGCTTTAAGCTCCAATGCCAATCACAAAGCCACCATTCACAGGGCACTTAAG
GAAACTGTCTTAAGCTTGCATACAAATTAGTTTAGATGTTACCTTCTAAAGAAAGCACTAATATTTTGCT
TTCTTTTTTCTTTTTTTAAAAGAATGACATCAGACTAGGGCATGGCAGTTCATGCCTGCAATCCCTGAAC
TTGATAGATCAAGACAAGAAGAAAGCCCAATTTTGTGGCCAGCCGGGGCTATAAAGTAAAATTCTAAGTA
GCTGGCTGTCTCTTCCCACCCCCGAGGCCATGGCTCTCTGAAAGCCCTTTGCTGGCTCTGCTCCATAGCC
GTGCTATTCCAAAACCCAGGCTCTTCACCACTATGCCTTCCATATTCAATCAGGCATCAGATGACTGGGG
CCATCCTGATCTGACTCAGCAACCAAGAACAAACCTCACTGATGGGTTTTCAGGTGATGCTGATGATGGA
GGTTGAAAGACGACCCTGCTTTACGGGTCACTGCATTAAGCTAGTCTTTCGATTAATAATTGATATGTAG
GTAACCTTTGAAGCTTTGTCACATGAGGCTTTGGTGTGACCCACTCCAGAATACCTTATGGAGATATTCT
GGAGGCTCCTTCAGAAACTAGCATCAGATATCCATTGCACAACACAGGAGACAAAAGATCCGAAAGACAC
CATTTGTTACCCTTCACACTTGATGGGACCTCACTTAGCCCTTGGCCATCAGCAAAAACAGTTCCTTCAA
AAACTGAGACAATCATCTACTGCTTTGTAAGAGTGTAAAATAGCTTTTAAAAACAAGTAGTTAATGGCAA
TCTCCGTCCACAAATTTCATGCAGAAGAGACTTAAAAAAAAAAAAACCTAAATCTTTCTGGATAATTTTA
AGAGTTTTTCAGTGCATTTGCCTGGCTCTTTGGCCTGTGCATGACAGTGATGATGATGATGGTGGTGGTG
GTGGTGATGATGATGATTTTAAAAAGCTAGCACAGGGTTATCCTGCCGTAACCTTAGCTGTGCTAAGGTG
CATGCTTTTTTACCATCTATCCTCACAGCTAATCCTATTAGCATAGACAATATTGTTCTTACCATTTTGT
GAGGACTCTGGGGCTACTGGTGGTTCAGGACCTTGCTCAAAATCTCCTTTGCAATTAGAGAAGATAGGAT
TTCTGCATCTTTCTTTCTCCACACCTATGCTTTAAGCCACTATGTTATTCTGCTCAATACTCTGCCCTGT
GTATTCCCTGTGAATTACAACTGGAAGCTTACTCCCCCTCATCTCCATTCCCAACACACACACACACACA
CACACACACACACACACACACACACACACTAGTGGCTTCTGACAAGGAATCTTGTGCTGTTTGCATCTTT
TCTTGTTTCAAGGTCTTCCCTTCCTAATCAGAGACCGATTATTACTGGCCACCTGCCAGTTAAATGACTC
GGTGGCAGCTATAGAACTGTTTCTCTGTTCTGTCCCCATGCCCTGAGATCTCTCAGCAAGCACTTGCCAA
ACTCCTGTGAGCTATGACGTTCCAAGGGCCATGGGTGTGTCAAGACACTGAGTGAAACCCAGTTCCCGGG
GTTTGTGAGGCTCCTGGTCCAGGTCCTCACTGCTGGTGCTCTCCAGTTTATGTTGTAGAGGTGGACTGGA
CTCCCGATGCCCCTGGAGAAACAGTGAACCTCACCTGTGACACGCCTGAAGAAGATGACATCACCTGGAC
CTCAGACCAGAGACATGGAGTCATAGGCTCTGGAAAGACCCTGACCATCACTGTCAAAGAGTTTCTAGAT
GCTGGCCAGTACACCTGCCACAAAGGAGGCGAGACTCTGAGCCACTCACATCTGCTGCTCCACAAGAAGG
AAAATGGAATTTGGTCCACTGAAATTTTAAAAAGTAATTCCATCCCCCTTGACAGTATCTTTTCTTCTAC
CCCTCCTTAAAAAGTAATAATTGCAAGTGTGTGTGTGTGTGTGTGTGTGTGCGCGTGCGCGTGCGCGTGC
ACTGAGGCTCAGAAATGTTTCTCATTTCCTTTGAAACAGGAACTCTCATTGGCCCATAGCTTACCAATAA
GCTTTCTTGGTTAGCCAATAACATCCAGAGACCTTGTCTCAAAAAAAAAAAAAAAAAAATCTCACCCTTG
TACCATGTAATGGCCAGTGATCCATCCACAGTAAAAAAGAAATAAATAAATAAAATAAAAATAAAAAATA
ACAAAAACAAATTTCTTTATTAAAAGAAAACTCCTAAATATTTAATTTTTCTTTTCTTTTCAAATGCAGA
TTTCAAAAACAAGACTTTCCTGAAGTGTGAAGCACCAAATTACTCCGGACGGTTCACGTGCTCATGGCTG
GTGCAAAGAAACATGGACTTGAAGTTCAACATCAAGAGCAGTAGCAGGTAAGGGAGCTGCCATGATTTCT
CAAGGTTTTGTGTGATCTACTTCATAACAAAAAGAAGTACAAACTGCCCATGGATTATCAGTGACTGGTA
ATGGAACAGCAAAGTCAATTCTGTATTTTTTTTTTCAACTTCCCTCTGCTCGCCTAAGGAAATAGCATGG
AGCTTTAAGCCAGCCCTCTGAGACTAGTTGGCTGGGTAGTTTGAATTCACTCATCCGAATTGCCTGGCCG
TGCTTCATTAAGGCTCACAGCACCATGGGGAGGAATTAAAAAGAAGCCACAGCTACAAATAACTGGCCAG
AGCTCGACCTTGTGTCAAAAGCAGAATTGAGTGCTCCAGGGCCTTGCTGTTGGAAGCCCTGGAACAGATT
CCAGAACCCTCTGAGGCCTTTCGAGATTTTCCTAGAGCCTTTTCAACTTCTTCCCCCCCCCACCCCCACC
CCCCAACCGTTATTCTGAAGTTCAGTTTCCCCAATGCACTTGGGCTTTTCCTAAAAACATCATCTCCCAG
GGCTGCAGAGCGGGTGTGTCAGACTGCTCTACAGCTGACTTTAGTCAGCAACTAAATCCCTGAGCCACAT
GGAGGGCAGGGAGTGAGCGCCCTGGCTCTCCCACTATTTAAATGAATAAACGGGGGACAGGGGGTGTGGG
GGGGACGACTCCCTTTTCCATCTTTATCATGAATTTGCTATGACTTAACTTAGCTAAGGAAAATAATGGT
TCAGGGTGACATCAGAGTCCTTGTTGCTTCTATGAGAATTTTCTGATCTGGTAAAGGTGGCCCCAAAGGA
GACCAGTGTACAATGCAGATGCTTACATATAGAAACATGCCAGACCTCTATGTGCTTTAAAAAATTAGAC
AAGCACGATACATTTTCCTGCTAGAGAAATCTTGCACGTGTTCCTGAAGAAACTAGTACATGGATAAGGG
TCAGTGGAACATTTTGCATAATTAGCAAAAGCATGCTCAAATGGAACATCAATGTGCATCTATTGAAATA
AATAACTCAACTTTCGTGTTCTGTTTAGAGAACTCCCCCACAATACTTTCAGAGAGGAAAAAGACGTTAC
AAACTCATGCATATATATTTTTCATTTGTCTTTAAAGTTGTGCTACTCTTTGGATAAACTACATATGTAC
ATATATGTCTGTGTGCATGTCTATCTTAAATATTCATTTGGAAAAACTCTAGAAGTAACAAACTGTGAAC
AATGTCTATATGTGGGACTCAGATTTGAGTAAAAAGGAGAATTGTGGCGACACGCATGTTTTCTTTATAA
TCATCTTTGTTGTTATAATTCTTTATAATTCAGCAGTTTCTCTTGCATTACGATATTTTCAGGTTCATTT
AGGCAGAGATGCAACTTTCAGTCTCTTACAGCTAAGCTTCCACTCTGCAAAGGGCAAGGGGGTTTTTGTT
TGGTTTTGGTTTTGGTTGTTTTATTCCACTGGAGAAATCCAACCAGCTGGACAGACTGGCCTCGGACTGT
AGACACCAGGCACTCTATCTCTGATCTATAATACAGAACTCATTCTGTCTTTGCCATCAGTTCCCCTGAC
TCTCGGGCAGTGACATGTGGAATGGCGTCTCTGTCTGCAGAGAAGGTCACACTGGACCAAAGGGACTATG
AGAAGTATTCAGTGTCCTGCCAGGAGGATGTCACCTGCCCAACTGCCGAGGAGACCCTGCCCATTGAACT
GGCGTTGGAAGCACGGCAGCAGAATAAATATGAGAACTACAGCACCAGCTTCTTCATCAGGGACATCAGT
GAGTTTTGCCTGGGAGCAGGTGCTAGGGAAGCAAAGACGATGTCTGCCACGTGTCCATGATACCCTGGGG
CATAGGATTGGCCTGCATTCTTTTCCATTCTCCTAAAAAGCATGATACAGACACTACCCTCTTCATCTTA
CAGATGAGGAAATTAAAGGGTTAAGCTAACCTTTTTCCAGATCTCAAATCTCACAGACAGCAGACCAGAC
CCAGACCTGCCCCAATGGTGGCACCTAATGGATTATGGGTTAACTACATGCTTAGTGTAAAGCAGAAACC
AAGCACAGGGAGGGTTCCCTGATTGCTGAGCCCAGATACGAGACTCTATTAAGCAGAATGACTCAACCAG
TAGGGACTTCCGGTCTGTGCAGTGCAGAAAGGGTTCTAAAATGTAGGATATGGCCAATAATGGTGACTTC
CTCGAGTGGAAAGATCACTCTCAGCAGATCCCTCACAGGTGCTGCCCTGATGCTCAGAGAAGTGAAAGAG
CTATTCCAGAGACTAGAAGGTGGAGGGCTCTGTCAGCCAAGCTCAGGAGCCTAAGTCCTAGCTCCAAAGC
CTGGCCAGGATAATAAAGGATGCTTGTCTTCTTCTGCAGTCAAACCAGACCCGCCCAAGAACTTGCAGAT
GAAGCCTTTGAAGAACTCACAGGTGGAGGTCAGCTGGGAGTACCCTGACTCCTGGAGCACTCCCCATTCC
TACTTCTCCCTCAAGTTCTTTGTTCGAATCCAGCGCAAGAAAGAAAAGATGAAGGAGACAGAGGAGGGGT
GTAACCAGGTAGGATTTATGCAGCCCATGTTGGGGCCATTGGAAAGATCCTGACAACCAGGTGCTTGCCT
CAGGACCAGAACTGTGTTAAGGGCTCTCCATGTGAATCACACACAACCTATACAAAGTCCCAAAGGCAGC
CAGTATTGCTGTCACCTCTCCACAGAGGCCAGGAAAGCTCAGAGAGGCTATGTTTTGCCCACGTTCTTCC
AGCTAGAGCAGTGGCAGAACCAGTACCCAGCAATGGTTTGATGGAGAAAGAGATGGTTAGCTCAACCACT
TCAATTCCTCCAGTATAGTCGATTGTCAATCCTCAACCAAAGTTGTTCATGATATAGAAACCTGGGATCC
AGGAGCCTATAATGATTGTTTAAACTGAGTAAATATAGTAAATGCTAAAACATTGATGCTGGGACCCACC
AAATAAGGATAATCTCTACTTCCTTAATATGAGCAATCATTTCACCTTACAAGATGTCCACCTTTACTGG
GCTAATATGGTGACTTTCTACCTTTCGGGTCCTTAGCACAGTGATCTCTTGCTAGGTTTGAGGAACACTT
TTATGAGCATTTAAGTCACAGAGCAAAGGATTCTTCCAACCTAGCATTGAGAATGTGATAGCAATTCAGC
TCAGAGATTCAACCACTTCCTAAAAGCAGGAGCAAAAAATGGGATTATTTTGAAACAGATTTTAACTGCT
ACCTAAGTTTCATCCCTGTGTGTATGTATTTAATATACCAATCTTAAATAGGTAATGTCTAGTGCTTTCT
ACTTGCTAAAAGCTTTCTACGCATTTTTACATAAATGTTGTATTTAGCTGCTGCAATTCTTAGAGGCAGG
CATCCTGGGACCTACTCATGAATAAGGAGAGACACAGAGAACCAGAGATATTTTCCTGAAGCACCACAGC
TGTTTAGTAGCATTGCCAGGATTTGAAGTCACTTTTGTAAGCTGACAGGCATGGTAATTCTTGATGTCAA
GACAGCTAAAGAAAGCTGGTCTCAGTCCCAACATCAGCTTCATATGGGAGTGGTTACCCATGGGACCTTG
GGGTAATGCTGCTACTTCAAGAGGCAGAAGGGGGAGCAAGCCATTTCTGTTGGAGTTTACTAAATAGAAA
AGTGAGAGTGGGGTGGTGGATGAATCATGCTGGAATATGAAGACCTGAATTTTAACCCCCAGAACCTCTG
TAAAACTGAGTAGTAATGCCTGAACCTGGGCTCTGGAAAGAGGTGGGACACATACACACACACACACACA
CACACACACACACACACACACACACACACACACCTCTGATTATAGCTTCTACCAGCAGGTGGTGCAAGGG
GAGCGCCCATGCCAGCCTGCCTTATATTGCCTAAAGCCCTTACTTAAATTTTCCTCTCCTTTGCAGAAAG
GTGCGTTCCTCGTAGAGAAGACATCTACCGAAGTCCAATGCAAAGGCGGGAATGTCTGCGTGCAAGCTCA
GGATCGCTATTACAATTCCTCATGCAGCAAGTGGGCATGTGTTCCCTGCAGGTGTGTGAGCAGGACCGTG
AAGCCCAACCACACCTGCACATTTAGTGGTCCTGGTGGAGGACAACAGGAGGCTTGTGAGGGAGGTGGGG
GTGGAATTCCTGGCATAGGGATTCCAGTCAGAAACACATTTGTTCATCAGCAGATGTTAACTTGATGTGT
ATGTTAGGTCAGGCACTATGCTAGGCCAAGGATATTACAATAAAGAAAACAAGCATGGGTCCTACCCTTG
TACAGGGAAAGGCAAGAAGGACCAGAGGAGGAGGAAATCAGGAGTCAGAGTATGGAGAACTTGGAAGTAG
ACAGAAGTTGGAACCATATCTTAGGGGTTCTGGGGAACCACTGAAGTCTTTTTAATAAGGAAATATCCCA
ATCAAAGCCCTTTTAAGAAAAATCTCTCAAGAGAGTCCCTGGCAAGCATGAGACCAGCTGAAGTCCAAGC
CCTGTAAGGAAGGGTTGGGCTGTCTCGCACAGAAATCCTCAAGACCCAGTGTCTGGGGATAATAATGTGT
GGTGTGAAATAGGAGGGTATTGAGGCTATACCTCTGACCAATATGGACTTACCTACCCACATTCCTTTAC
AGGGTCCGATCCTAGGATGCAACGTTGGAAAGGAAAGAAAAGTGGAAGACATTAAGGAAGAAAAATTTAA
ACTCAGGATGGAAGAGTCCCCCAAAAGCTGTCTTCTGCTTGGTTGGCTTTTTCCAGTTTTCCTAAGTTCA
TCATGACACCTTTGCTGATTTCTACATGTAAATGTTAAATGCCCGCAGAGCCAGGGAGCTAATGTATGCA
TAGATATTCTAGCATTCCACTTGGCCTTATGCTGTTGAAATATTTAAGTAATTTATGTATTTATTAATTT
ATTTCTGCATTTCACATTTGTATACCAAGATGTATTGAATATTTCATGTGCTCGTGGCCTGATCCACTGG
GACCAGGCCCTATTATGCAAATTGTGAGCTTGTTATCTTCTTCAACAGCTCTTCAATCAGGGCTGCGTAG
GTACATTAGCTTTTGTGACAACCAATAAGAACATAATATTCTGACACAAGCAGTGTTACATATTTGTGAC
CAGTAAAGACATAGGTGGTATTTGGAGACATGAAGAAGCTGTAAAGTTGACTCTGAAGAGTTTAGCACTA
GTTTCAACACCAAGAAAGACTTTTTAGAAGTGATATTGATAAGAAACCAGGGCCTTCTTTAGAAGGGTAC
CTAAATTTAAAAGAATTTTGAAAGGCTGGGTATCGGTGGTATATGCTTTTAATTCCAGCACTCAGGAGAC
CAAGGCAGGCAGATCTCTGTGAGTTTGAGGACAGCCTGGTGTACAGAGGGAGTTCCAGCACAGCCAGTGC
CACACAGAAATTCTGTCTCAAAAAC

Protein Sequence : Show Sequence

>gi|6680397|ref|NP_032378.1| interleukin-12 subunit beta precursor [Mus musculus]
MCPQKLTISWFAIVLLVSPLMAMWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIG
SGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCS
WLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELAL
EARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEK
MKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG) , MVA/IL-15/5Mtb vaccine

25. Il15

Gene Name : Il15
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16168
NCBI Protein GI : 363000984
Genbank Accession : AC156993
Protein Accession : NP_001241676
Taxonomy ID : 10090
Chromosome No : 8
Gene Starting Position : 82331623
Gene Ending Position : 82402585
Gene Strand (Orientation) : -
Protein Name : transcript variant 2
Protein pI : 4.96
Protein Weight : 17600.72
Protein Length : 162
Protein Note : Also known as IL-15; AI503618

DNA Sequence : Show Sequence

>gi|372099102:82331623-82402585 Mus musculus strain C57BL/6J chromosome 8, GRCm38 C57BL/6J
GTTAAATTTCAATTGATAAAATATCCATACAACTTTATTCCACATCTTCTGTTATATTACAAATATGTAC
AAATGAAGCAATAAATTAAAAATAAGCAAGCAAAAATAAGTCTGAGACGAGCTCTTTTCCTCTCTTCATT
TCCAAGATTTCATGTGATCCAAGTGGCTCATTATCTCCTTGTTAGGAAGCTTAGTTTGCCCAGCAGATTT
CAAATGCATGGAGCTGTGCTGCCTCTGAGCAGCAGGTGGAGGTACCTTAATAACAGAAACACGGAAGAGG
CTCGCATGCAGTCAGGACGTGTTGATGAACATTTGGACAATGCGTATAAAGCTTTGCAAAAACTCTGTGA
AGGTTTTCTCCTCCAGCTCCTCACATTCCTTGCAGCCAGATTCTGCTACATTCTGCCAAATAAGGAGGGA
GAATTAGATCTGCAGTCAGAACACACTTCTTCGTTGGAGGATATCATACTATTGCAACACATGGTGGAGT
CTTCAGACATGAAAACATTGGCAAATGAAATCAAGTTTTGAAAAATAGTGAGAAAGAGTCAAGAGATTTA
TAGGGGTTGGGTATTCGTACGTTGGATTAAAATCTTTGCAAATGTACAGGACATTCATGTTACATTGTGT
CCTTCCTGCTTCAAGCCCATCACTGTGTTGTTGTTGCTGTTGTTGTTGTTGTGGTGGTGGTTTTGATTAT
TTTTCCTTGTTGTGGTATTTCTTTTTCTTATCTTTCGTATCTCTCTCAAAGTCTGTACTTCATGAGCACT
TGTCTTTCTGTTCTATACATATAGTGTGTATGTGTGTGTGATATATATCACATATATAATAGTTAGATAT
GCATATATCACAGACTTTTGGCCTTTTTACACCACTTTTAGCCTTTTACACTACTGTTGCCTAACTACTA
TTCTTGGGAAGCATAGCCATCTATCTTTTGTTTCCCAGCAATAAACACAAGGCTTGGCACAGAGTAGCTA
ACTGAATATTATTGGTTGATCTGAACCACTTAGGCAAATGCCATAAACATAGCTTTGGGGACAGCTAGAA
TACTATAAGTGTAGCCACCATGAATAAACATTTTAGAGATCATCTGAACCACGGAGGCTGTGCCAGCAGA
AATCAAAATTATATTTCATGTTCATTAGGCACTTGGAATTCAGATTCCCCAATATGTATAAGGCACACAA
TGAATGCCAGACACTGCTGTAATGCTAATAGGATTCTCTAGTCGGTCCAGAAACAATTTGAAAGAGGAAA
GCAAACATGCTTAGTGGTTCATCGCCACTGACATGTAAACTGAAATTCTACACAAGTCTCCTAACCATGC
AGTCCTGTTCATTCTTCCAGTATTTATTTAGGCCAAACTGTCACTTTGGGGGCTCCAACATGCAGCCTAA
AGCTCAGCATTGATACTGTGATGAGTTATCTGTTACCATTAATAGGCAATGATTCACTTGGTAAGAATCC
CAAGTAACGGATACCTGTAAGTTTATGTACTGGGAATTGTAATAACACAGTCAAAAGAACTCTAAAATGT
ATGTACTCTGATGCTGACTTTCTAATGGTGACAGATGATTTCAGCTACTGACTCTAGGGCATAATTATCA
GTGTGGGGCATATATATATATATATATATATATATATATATATAGGTGGGAACAGTGAGCAAGGCTGTAG
GGAAAGCATGGCAAATATGAATAAGTGATATCACTGAGGATGTAGCATCTGAGCAGACACTGAAGCAGCC
CAGCATTTGGGAAGAGAAGCCAAGACATCTCAAGATCACTGTACCTCCAGTTGTGTCTGCCTAAAGACGC
TCCCGGAGTGTCCCTGACATCCATTCCCTGACTTCTGTGTAGCATACCACCTTATTGACTAACTGCTCTT
CACAAACACACACACAGACACACACAGACACACATAGACACACACACACACACACACACACACACACATA
TTACCTCCTTCCTCAGAACATGTGCTTCTTAAAGGGATATGTTGAAGTGCATACTAAACGAAACAATTTT
ACTATTCCCCCTCTGATGATAATAATCCTTTGTTTGAATCTTGTTTTCTCACTATGGTTGTCCCTCTCAG
GCCACAATTCCTTGTACCTCAGGACATTTGTGTCACTTACCCTTTAGCCAACCACAGGAGTCACTAGTAT
TTGTTTCTTGTAACCCACTCCTGCTCATGTTGTCTGGGATGTTCTAAATAATACTTGAATTGTTAACACT
TTTCATCATTCTAATTTTTCCATTACTTAAACGTTTAGAAGGTGAGAGGGCAGAAGCTGGAGTAGAATCA
CAGCTCTAATGCCTTTGGCTGCACAAACTGGGGCATGTGGTTAGCTTTCTGCTTCCCATGTGCATCAACA
TTCGAACAAGAACAGAAATAGCCAGCCATGAAAGAAGGGAAGTGACCAGTCTCTGAGACTGATTTTGAGG
ATTTAGTACCTTATCAGTCTAAAGTATAAAGATCAATGTGTGCTATTAAGACCTAACCCAATAAATGTGA
GCTACTGTTTCTGATGCTAAACACTTGATCACATTAATGTCTATCCACTGCACCCACTTGCCAATAAGAC
ATTTTATTTTAAATCAATATGACTCTGACAGGGAGCACACTGGTTGGCATGATTCAGAAATGTCTGTTTA
CCAAGGAGACCAACATGTTTAGGATTCTGTGCATGCCAGCACTGGTACCTCTTTTGGACACCACTCCACT
GGAATCAAATTCTCTTCTGTGTGAACCACAGGAGACTCTCCTTATATATACATGCCTTATTTCAAGCAAG
CCAAAACTAGTCAGATGCAGCTCTCTCTGGATGCTGGACTACTCACCTTGTTAGAAGACAGAGTGCTGTT
TGCAAGGTAGAGCACGTTTCTTACTGTTTCATTAAGAGTCATGTTACTGTACTCATGTAAAATAACCTGC
AATTCCAGGAGAAAGCAGTTCATTGCAGTAACTTTGCAACTGGGCTAAAAAGAAAAGAGCCAATTTAAAA
AAAATAAACAAAAAACCTTTTCCCATTATTCATTTTCATATTGATAAGACTTTGATTGAAAAAAAAAAGT
CAAATCCATCTAGTTATTTTACAACGAAACGTAATATCCACACTGCTTAGTTTTAATTGTCAACTTGACA
CAACTAAGAACATAGTCTTAATGGGGACCACTTCTCAGATAACCTGTAGGCATGTCTGTAGAAGCTTGTC
TTCCTTGTTAATTCATGGAGGAAGGGCCAAACTAGTGTGGTGGCACCATTCCCTAGGCAGAGGGTCCTGA
ACAGTATAAGACAGAGGAGAGCCACCTGAGAGCGAGCAAGCAAGCAAGCATTCTCGCAGTTACTGTGTCT
CTGTTCTTCACTGTTAGCTGGATACTTTAAGTTCCAGTCCTGACTTCCCATAACTATGGACTACAACTTG
GAACTGTAAGCCAAATAAACACTTTCTTCCCTAAATCACTTTCCGACTGTGTGCCAGCAGGGAGTTTTAT
TTACACAGGAAGTGCCTTTCTTTTTCCATTTGATTTTGAAATAAAAACCAAAACCACTAATAGAGAAGGA
TAGTACAGAAGGAAACACAGCATAAAAGGCTACACCATCTCTCACTAAACGCCCTCTTCCAACGGTTGAA
TGAGAAGCCTAGAAATGACAGAACATATGTAAAAAGTGTTTAGGGTCATACTACATGTTCACATCTTACA
GAGTGGTAACTGTAAGTCACACTGTCCCTATAGTTCTCAGCAGAAAGAATTCTAAGACATTTGAGAGAAA
ACCCAAAGCAAAGGCAGTGCCCACTTATGCACTGGCCAAAAAGGGACTGTGGACAAGAAAATGTATTTCT
TGTAGGAGTTACTTCACAAAACCCCAAACTTAAAAAAAAACAAAACCAAAACCAAAAAAAAAAAAAACTT
AAGGAAACATTAAATGGAAAATTAGCTATGCCTGAGCCTCAGTATTCACATCCACACTTCAATACTGGAA
GCTTTGCCTCACCTTTGAGTTGCAAGGCTATCATCCTGCATGGGAAAACTTTCTAATTAGGAAAAAAAGC
AGAGAAAAACAAAAAATATGCTAAACTCAGATGAGGAGTTATCTAGATCTCACACACTTGTTTGGTGGAC
ATTTGCTTGTCTGAGTTTCAGGCTCTACCTTCTATGCAACAAACCATGAGCAGAGAGCACACACCTTCCA
CCTTGGAGAAGTTCCAGGTTCCAAACACAAAATACACAGTTTGGTTTTGGTTCTAGGTTCATTGCTTTCT
CTGTCTCTGTGATAAAATACTCTGACAAAGAGTAACTTCAAGGAATCCGGGAACCAAGATGGCTCCCCCA
GGTGCTCCGGCAAAGCTCCTTCTTAGAATTGGGAACAAAACACCCATGGAAGGAGTTACAGAGACAAAGT
TTGGAGCTGAGACAAAAGGATGGACCATCTAGAGACTGCCACACCCGGGGATCCATCCCATAATCAGCCT
CCAAACGCTGACACCATTGCATATGCCAGCAAGATTTTGCTGAAGGGACCCTGATATAGCTGTCTCTTGT
AAGGCTATGCCGGTGCCTGGCAAACACAGAAGTGGATGCTCACAGTCAGCTATTGGATGGAACACAGGGC
CCCCAATGGAGGAGCTAGAGAAAGTACCTAAGGAGCTAAAGAGGTCTGCAACCCTATAGGTGGAACAACA
ATATGAACTAACCAGTACCCCCAGAGCTCGTGTCTCTAGCTGCATGTATATCAGAAGATGACCTAGTTGG
CCATCATTGGAAAGAGAGGCCTATTGGTCTTGCAAACTTCATATGCCTCAGTACAGGGAATGCCGGGGCC
AAGAAGTGGGAGTGGGTGCGTAGGGGGTTGATGGGGAGGGTATGGGGGACTTTTGGCATAGCATTGGAAA
TGTAAATGAAGAAAATACCTAATATAAATAAATAATAAATAAATAAATAAATAAATAAATAAATAAATAA
ATAAGCAACTTCAAGGAGAAAGAAGGTCTGTCTGTTCACAATTTCAAGTCATAGCTCATCATCAAAGGGA
AGTCAAGGCAACAGACCTTAAGGTAGCTAAGCAGAAGCATAGTTAAGAGCAGAGAGAATGTGTTCAGCTG
TGCTCAGGCCTTCTCCAGCCTTCTCTACTCTTTAGTCCAGGGTTTACCCCATGAACTGATGCAGCCCACA
TCAGAGTGGATCTTCTCACCAGGATTAAAGCATTCGGGAGACATGCCCCCAGGGGCAAACCTCACTGATG
TTCTTTCTAGGTAACTTTACATTGTGGGAGGTTAACAATTATTTAAATTAACATTTTCTGAGCAATGGAA
TTGGAGATCTATTTCTTCATTTTAGCGATAGCCTTAGAGTAAAAGGAGAAACCAAGTGACCAAATGCCAT
CAGCAACATCAGTATACTGTAATGAGGCTGCCTCATCCTTTAGAAATTCTAGTACATCATTTGGATTATT
ATTTTATCTCACATAGAGACTAGTGGAAATATTTCTTACAAGTGTAGAGATCCATACAAAATACAAAGCA
AGGATGTCTTTAAGCAAGTTTCAGTACATGTGCTGAGGATGTTCACACACTACAGCACTAAAGGAATGGG
GTAAAGAAGACATTTAGATGGGATCATTTAGTCCCATTCTAGCATCTAATTATATGATGATCTAACTGCA
TCACAGAAATATGTAACAGATTTTGTTTGACATTGATGAAAAGTTGTTTTTTTTTATTCCACTGTGATTT
GACACCATTATAATAGTAGCATTTATGTAGCATTTTTCCATGAGTCCCAAAAATTCCACACTCCCTCAGA
AATATCATGGATCAAACACTAAGAGAACCCACAGTAAGTTAGATTTTAAAACTATAACTGTTTCAATGAA
GTTATGGAAATACGGCTGATGAACTGGAAAAATAGCAATTTTGAAAAACCTAACTATACTCACATGAAAG
TCACTGTCAGTGTATAAAGTGGTGTCAATATGAATAGACTGAAAAAGAGAAAAGGACGTCATATGAAAAA
TCTAGGTATGATACTAGCAACTTCCTGTAAACAATAAATTTTGTCCCTTAACTTAAACGTGAATTTTGCA
ATCATTTTAAGCTCCTGTTATTATAAGAGAACATATTGAGAGACCGCCTGACCCTCCTCTGCATTTCCCA
AGCAGGGAAATTCATATTGATGTAACATTTCTCTCTTCTCCAGATTTACCAGTTTTCATCCCCCTGTCCA
ATCCTAGACCTTTAACATTTCAAGAATGTGAAAGAAATGGAGTTATATAGAATGCAACCTTTTAAGATTA
GCTTTTTAAAAAATAACATTTTGAGAAGTTCTGTTATAGGGGAAAGACAGCAGAAGGGCCAAGGTTTCTT
CACCCCTTAATCTGACTCCAGTTCCCTTCATGGTTAATGTGCCTCACACACCATATGTGATTGTTATACC
CAGTGTGTGGACACCCCTACATAGCTACAGTGTGTGTTTAATGCCCTGAAAGTCTTGGTTCCAGAATAGA
AATAGTGAGCCACTGGAGGGCAACAGTCACTGGGATTGGACTTTGAAGGACACTGTAGGACTTTTATCTC
TTCATTTTTTCTTCATTTTTCTTTTCTAGAAAATATGTGAGCAGATTGTTTTGCCATTAGAAGATGGCAG
TCCCACCAGAGACCCAAAAATAAATACTTTAGTATATAATATAATAATTATATAATATGTATTCTATGGT
TTTTAAATATATATATATATATATATATATACAATGGCAGGTACCTAGCATTCTAATGGCACACATTGCA
GTGGAACTATGTTAAAGAACACCATACTCTCCCTACTCACCCCTCCTTTTTCCGTCTGTCTTTTTTTTTC
TGTAATTTTATCATCTCTCCAAGGAAAAATAGTTGGACCTGCCCAACATGAAACATGTTTAGACTGGGTT
CTTTCACTGCATGGCCGGCTTTTGGGGTATCTGTTATCTGTTTCCTTTCATGCCTTAGCCACATCTATCT
CTTGATCTACTGAACGGGTACACCAAAAGGAATAGCTTGGCAAATAAAAGTGGTGAGAGATTTGTTTTAC
TCCCAGAGGAAAACTGTCTTTCCCTCACTGGCAAAAGATTAAGTATTTTAGTAATATTTTATATTAAGTT
AAAGAAGTTGTCCTTTGTTTCTATTCTGCTGAGAAATTTTATCTTGAAGTAGCTCTACAGCAGTTTGCTG
AAGCTGCTATAACAATTTCATATATGCTGGATAGCGTAAGTAAAGACATGTACTTTTCTCGAATTCTGAA
GACAATCAGTCTCAGATGGAGATGTTAGGAGTGGTCTCCCAAGACTTCTCACTTTGCCTTATCAATTCTT
ATCCTATCCTATCCTCTCTCTGTCTCCTCAGGGTATCTTCCTCCTGTGGATACCCTTCTTGGAGCATGGT
TTCCTGCCCTTCTAAGGAAACTGGACATATTAGATTAGAATCATCCTAATGGACCTGTTTTCACTTAAAT
TTCTCTTTAAAGACCCTCTGTCAAAAGACAGTCACACAATGGGATATGGACATTCATTAAAAAAGGAAAA
TCCATCCTGTATAAGAGGTACTAAATTTATCATGTCTTTTCTAAATCAATTGATATGATTTTCCTTTTTG
GCCCCTGATATGTCAGCCTGAGTTGACTGATAGTTCTTGAAATAATCAAAAGACTTGCATAACTAGAATA
AATTCTAAATTCTATACAGTGATGATATAGGATTCTTTTTATAATACACTGGTAGATCTGATAGGTTGAT
ATTGTACTGAGGAATTTTATACTAAGTACATGAGAGTTGCTTATATTTGGTTTCATTTTTTAAAATGTAT
CTTTATCAAGTTTTCACATCAAGGTAAGATTAGTCTCCTAAACTGAATTAGAGGGACTTGCTTCTTCCTG
GGGGGGGGGGGGAGGCATGATCAATGTTTGAAGTAAGTTTCAGGATGATAGAGCAAGGCTAGTCGCTCCC
AAGTGTTCTTCTAGGAGGCTCTCCATGGTGACCTGAACAGTGTCAGGAGTTGTAGGAGCTCTTGAACCAC
CAGTTTCATCATTGTTATGTTTTGGGGAGTTCATGATCTTTGAAGAATGTTTCCATTTCCTGTAAATCAT
TGGTACAGTTATAAGACACTGAGGGAAACAGTGACAAATGTTATCCCCACAGATCCTGTGTCAATCGCCA
CCAATTCCATCCATGGTGCCTGGTACCATCTCTATTATTGTTAGATGTTTACAAATTTTATTGATTTTTT
TTTACTAGTGCCTTTATGTTTCATTGATTTGCCCCCTGACTTCCTGATTTCAGTTTCATTAATGCCTGCT
CCCAGATCTATTTTTTCCTTCCTTCTACTCATATTGAATTCATTTTGTGATTCTTCTAATTTGGTGAGAC
AGAACTTCAGGTGCTGGCTTGATGCTTAGTGCTTTTCCTCTTTTCTAATTCACGCATTGCATTTTTAAAG
TTTCCTCATCTTTAGCAGTTCACATTGTAACAGACAGGGTTTCATTTTATTCATGTCTGTGAGGTTGTTT
GCGTATCTTTGGGAATTAGTCTTTGACCCATGTGTTACTTAGAAATACGTTGTTTAATTTGCAAATTTGG
GGGACTTTCCTTTTGTATCTTTTTATCTCCAGATTGATTCCATTGTGTTCTAAACATATTTTCTGCACAA
TCAAAGTTCTGCTGAGTTCAATGCCCCTTACTTTATGGCTCGGGAGATATTTAGTCTTGGTGACTGTTTT
ATGTGAATTTTTAAAAGTTTGTTTTGATGTCGTTGGGTAGAATGCTTCATGCATAAAGTCAAATCCTATT
AATTGTATTTGTTATGGTTCCTATGTTTTTGGAATATTGCTACTTAATAGTTTAGTTAGTTATTGGGCAG
GAGATGCTGAGATCCACAACTGTAATCATAAATTTGTCTGTTTCTCCTTTGAGCTGTTAAATTTTTGTGT
CATGAATTTTGAGATTCTGTTGCTTGGTGCATATATATTAAAAATCAATGTTTCTTCTTCATGAATTTTC
TTTTGTCATTAATGTTGCTCTTGGCATTGGCAGCTCCATTTTCCATCATGTTGGCTTATCTAATACTAAT
ATAAGACTAATCCTGCTTTTGATTGTGACTTTCCTCTTCTTGGTATGATTAATGCTTTTTCCCTGTGCCT
GGATATGCTGGATATTATGCTAGGAGACTCTTGTCCTACTTAAGTATTCTATTTTAATATGCAGCCAATC
ACCTATTGAGATGTTTTGTGCTCAGCCTGACTTACTGTTGTGTGCTGTGATTCTAATGGCAGCTTAGCTT
TCAGAACCTCTTCAAACCTTCCACTGTAATTCTCAATCACTGCAGTAAGAGTTGCTTCTTCATACCAGGT
TATGTGGTTCATGGGGACAAAATATATATTTCAGGGTTGTCATTTATGATGAGATTCCTAATCTTTCTTG
ATTAGATTGAGCCGAACCTGCTACTAGGCCATCTAGCAACTCTAGGCAGGAGAAGATGATCTACAATGCC
CAGAAAAAAAATACCTCATTCTAGGGCTAACACCACATTGGGTCCCCTTCCTGTAACCCATTTTCCAGAT
AAATAAGTTTCTGATTTGTGAGTTGGCAATTGAATTTATTTGTTATTCCTTTAGTAGTTCCTTGACATTT
TTGTACACCAGTTTTGATACTTACTCTCCTTCTAACTCCTTCCATGACCACATCCCGTCCCTACCCACCA
AACTTTGTGCCCTCTTCGTTTCTTCTTTCTTAATTAAGTATGATCTGTGCTGACCATATACTCTTATTAG
ATGTGTGGCCATCCACGGTTGCTAGGTAACCTAACCAGGGACAACACACTTAAAGAAAACTTACTTCCCT
TCTCTCAGCAACTACTAATTGCCAGCACTTCTTCAGTTGGGAGTGAATTTTATGTTATTTTCCACATTTC
ATGTTAGGATTTTATTTGACTTGAGATTGTGTGGGTCTGGGGTATACTGACAAATGTGTATGTGTTCACA
TGTGCAGCTGCCCTGCTGTGTTGATCAAACACTTTCCTTGGAGTCATCTACTACCCTGGGCTCTAACAAG
TTTTCCAATACCTCTCCTACACAGATAGCCTAGTCTTGGGAAGAGGGTATGTGATACAGAAGTCCCATTT
AGGGATGAGTGCTCTGCAGGCTCATATCCTCTGCACCTTGACTGGTTGTGGTCTCTGTGTTAATGACCAT
CTAGAGCAAAACGAAGCTTCTGTGATGAGTTTTGGAAAATGCACTAATCTACAGGTATAATGATGTCATC
AGGAGTCACTTTTATGCTATGCATTTAGCGAAGAAATGATAATAGGTTCTACATTAGAGTCCAGGATCTG
TCTACACATAGTTCTTAGCTCTGATAAAGGTGTAAATCTTAACAGTAAATAGAATTTTAAAACAATGTCT
TAGAAGGCTTTGCAATGTTCATATATTTCTTCACAAATATTATTATTTCAAATGGTATTTCATAGAAATT
TGTAGAAAAACATAAGACATGCATACTTCATAGCCATTACATGTTTTCTTTCTGGAAAAACAATGATATC
AATGACCTAACCCAGGACTTGGCTCTATGTGCCATAATACTAACCTACCATGTGGGATGTAGCTAGTGGC
AAGGCTCTTGTAGACAACTAGCCACTTTTAAGTTGGATTGGAGAACTGCTCAATACTACTACTACTACTA
CTACTAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAAATTATGGCTGGTACTATATC
CCTGAGCAAAACCCCATGGCTGGGCAGCCATACGCCCTAGGCCACATGCCACTGATATTTTCTCAGTGGT
TTTGCTGTCAAATTACCTTCTCAGTATTTATAGTTGTGTTGGTAGACTTGGTATCCGTCGGTAGACTTGG
TATCCGTCACCCTTGGTCAGAGGCATTTAGTCCCCCCCCCCAGTAGTAAGCAATCAGTGCTGGGGTATAA
TTGGTCAAAGTGTTATGAAGAAGAGATTGACTGTTCAGCACTAAATAAGACATTTTTATGAACCCCACTC
CTAGTCCTGCCAAGACTCTGAGGATACTGATGAAAAGGATGCAGAAAAAAAGTTAAAAACCAGAGGGTGA
GAAGAAGGACAATGGAATACTGTCCTCTGGATCCAATATGGCTGTCACATGCATGAGCTCACAGTACTCT
GTTATCTGTACAGATCTTGTCTGCCAAAATGACAGCTTAAAAGGGGATGTGGTCTCTTGGCCCAGAACCC
CCTTAACAACCTGTTGGCATGTGATAGCTACTGAGAGAAAATGTATTATTCCCTTTTGTTGGTGTCTCAC
TCTTATTTTTCTGTTTCAGTGGATGGCAAAATGGCAGTGTACATACTGGTGACATTAATTGGACTTAATG
GGTTATCAAAACAGCATGAAACAAAAGATGCGAAGTTGGGGCAGACACAGTGGCAAAATAGAAGAGAGGT
CTTAAAAGGAGTAAAATGGGGAGTAGATACAATCATATTTTACTACATATATATGAAATTCTTGAGAATA
AAAGTTATAATACAACAATATATATCAAATATTAAAACAAACAAAAAAAGAAAAATGTGTACTCACTTGA
ATAAGGCTTTCAATTTTCTCCAGGTCATATCTTACATCTATCCAGTTGGCCTCTGTTTTAGGGAGACCTA
CACTGACACAGCTGTAAGAAGACACAAAACCAAGCTCTTTGGAGGAGGGTACCATTGTCTCACTGAGTAG
ATAAGCAACGGAACAATCAAGATCAATTATCTTCTCACTAGGAAAATGTCTTATTCTGTCTCTGTGCATC
TGACAGCCTGGTGGGTGAATGGAAATACCAAAAATACAGTATAATTTTGATAGTCTAAGCTAATTTGAGG
AAGACTGTCCAACTGTCCAACTTAGTGATCAGAGAAAGGCTAAATGGATAGCTTATAAGTAATCACAGAT
GTCAGGAGTTATAATGGGACAAGCTAATAATTAGCAGGTGATCTTCCTGAGATGGCCTGCTTCAGATTAT
TACATAATCTGTTTATCACACTGATATTGAGATAGCACCTGATCTGTGCCATAGCTTAATGAATGTTTCT
GAAGTGATTATTAGTTGTCTAGAAGTACAGGCTCCATGAAACTTTTAGGGCTAGTAAGTGTAAGATTTTG
AACACTTAACACTTCCTCTTAACAACTGTGAGAGTAAATGTCCCTTTTTCATGATGCCCACAGTAATCTG
ATGCTAGGATGATTTCATAGTCACTGAAGATTTCGCTAAATACAATATGGGTGATTACAAGTTTATTCAC
TTTGGTTAAAACTCACATTTAATATCCCGAATAAAAGACACACTGATCACGTGACTGTGGAGTGATTCTT
AAAGCCCCAGGAAAAACAGTTATTCTAAAAAAGACCAGGAACAAACCATGTCAGCCAATGACAATCTGTC
TAATGAGGACAACTTACATACATGATATCAGTATATGGAACAGTTATAGCAAAAAGCTCAACTGCTTTAA
GGGATAGAATACTCATATAGAATACTGCCAAGGTGCCGAAATCCTCAGTTAAGGATTTGCACGTCCCCCA
AGCAGCTCCACAGTCACCTGATATCCTACGCTGAAGAGTGAAGACCTATATACAAGGAAACTGAACATAA
TACAGTTTCAACATCAAGATACTCAGGGACAAATGATTGTGGCCACTAACTTGATAATAGCTATGACCAA
AGATGAAATTACCCCAAAATGAAGACATGAATGCCAGCCTCAGTTAAAAAGTGACTGTTTAGAAGGAAAC
ACAAGTAGCACGAGATGGATGTATTCCTCATATATGGTTTCTGGAAAACATAAATGTAGACAAAATGGTA
AAACATCTCTAGTTAAAAACATGGAAGACTGAATTTTATTGTATTTTAGGTTCAAAATTGTAAAGGTGTC
ATTTCACAATCAATACTCTTTATGGAAACAGTGAAATTTCAGTGGCTCTTCTGTTTTGTTTTATTCTAAT
ATCCAGCTCTCTGTATGTACAATATCTCCAGTAGCCATTTCTCCAATTGAAGGTCAATGATAAACTGACA
TTCTTAATTACTGACATGTATACTCTTGGGACTACTGAGCTTTTTCTGTCATTATCCTCTTCCTCTTGCC
CTTCTGTCTTTATTAATAGACTACACATTATAAACAGCATAGATTAGTAATGTCACACAATAGCAAGCAC
ACTAGTTTTACATTCATTTCATACAAGTATATGGAAATTAAAATACATATTATCACACAGAGAAAAACTG
GATAAAATTTAAACCAATAAAAGTATTTGGGCCAGGGCTAGGATTGATGTCGTCGGACCTAGGATGAGAG
AGACAGGAACAAGGGATTCTGTAAGCTCAGGACTACCCTGGTGAGTTCCAGGCCACCCAACGCTACATAG
TTATGAAATCCTGTCTCAAAAAGAAGTATATCCTTTTTCATTCTAACAGCTCCTACATGAAAACTTAATT
TGGTTATTTAGGACCTTATTTGTCTGAGATAGACACAGTTTTAATTCTCTGACCTGCCTTGTCAGTGCTG
TCAGAGATTTTATAGTTACAGAGAGCAGCGCCAGTCAACACCTCAAACACCAGGAGTCTGTACTCTGTGT
AGACTCTGCTGAAAGATGCCTAACACCAGACCTTGCCCTCAGAGACACAATGTGCGAAGTGAATAGACAT
TTAGAGAACAAAAGAGACTGAGTGAAGTGTAATAACACCTTAACAATGGCACAAAGAATACAGGCATACT
GTTAGTCTTGTTTTCCAAAGCCCATGCTACTTATTCTTAAAATCAAGATAAATCTTGGAGAAACAGATGT
CTGTCGCAGCCAGAGATGTTTAGAGAATAATCATGCGACTTATTATAAAGTAGAACTGAGATTAAGGTGA
CCTTATAAGAAAAGCCATATGCTATTTTTGTCTGCTTTTTTCCTTACCAAAATTTTCATTACTTAATGCC
CAGGTAAGAGCTTCAATGTTGAAGATGAGCTGGCTATGGCGATGGGCTTCCAGCTGCCATCCATCCAGAA
CTCTTCCTGCAACAGAACCAGGATGAAGGATGACTATCAAAACACAAGCGAGTAAGCCAGAAGCAGATAG
AGAAACGCCTGGTGCTTATGCCACATCTGCTTTCCATAGGGTAGGCTTTGTGTTCCTTAACACACGCATC
AGATTTAGCCCTCCTGAGAGCATCATTTGGAAAATCTGATCTGGCGTCACAGAAATCCCATGACTGCCCG
GACTAAAACCATTTTAGTTTGTCTGAATAAAAGAACAAAGACATTATTTCACACAGTGTCCCAGATCTGA
CAGGTTTCAAGGCCATCTCAATTTGAATAAGAGCAATCAAATACAAAGATTCACATAACCACCAGCTGAT
AGTCGAATTTACAACAACAGCCTCATCTACAGACTTCACATAGATGTCTTAGGTATGTTCTTTAAAATAA
AGTTTAATGAGAACACCATTTGTGAATTTTCTATATAACAAAATTATATTTTTTTCTAAACCAAAACAGG
GGAACATGTGAAGTTTAAACACTCCCATCAAACTCCATTTCATTAAAATATTTAGGCTCAAAATATCTCA
AATATGGATAGTCTTGCTGTCAAAAATGTTACGCATTAGTAAACCTTACCAATGAAAATATCAAGGTCTG
TTGCTTCACTGATATAATTTATCACAATCAATTATCTCAATATTTTACACATTTCAGATGCTTCCTAATA
TTAAAATATTTGCTATAAATTATGTGCAATGTATAACTCTTATTTTTTTACATTTACTATGTGTGTGTGT
CTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGCTCATACACGTGCCA
TGGTACATGTGTGGAGGTCAAAGAACAAATTGTGAGAGTTGCTGCTCTCCTTTCACCACTTGGGGATCTA
GGAACAAACTTAGGCCATGAAACTGGGCAGCAAGGACTGTTACCTACTGAGTCATCTTGCTATCTTGTTT
ACACTCTACAGTGTATATTACATCATTCTTAAGAGAAAATAAACCTAACATTTACCAGGGTATGAAACAG
TACACTAGACTGTGTGGCTAGTACAATGCTTCCATGAAGTCTTCACCCCACGTTCCCGTTCCCTGTGACA
CTGGCATTAGACTTCCCCCTCCAGCAGTGGTTCATTTATTAAACAGAGAGGCTGGTTTACACCTAGGGCT
GACGTTTGCCCCTAAAGCCTAAGTCCCCTGAGCCCACAATGGAAAGTAAACGCAGCAAAGACTTGAGAGT
CAGCCAGGGTTTCCCACTCAGCCCGCAATGACTTTTGTGAGGTCTGTTCTTAACTGCCTTGCTTTCATTT
CAGTAAAGCTTGATTAACTTCTCCTCAGGACAACGTGTGTTCTTTGTCTGGAAAAGTGCTCTGACTGTAG
TCTTTTTGCTAAGGACCATGGCTTTCCAGTTCACTGGTAACAAGAGCCTATTATGTTATCTCTGTGTTAT
GTCTGTGTATGCTCCAGGCATGGCAACCCACAAGTGACAGATTACCTGGAAAAGGCAGAGAAATGGATTA
AAATCTGCGTATGACAAGTGAGCACAGACCAGAAGGGAGCAGGGAGCTTGGAAGTATTAAGGCCAGTGGA
ATGTGAAGCATGCCCTATTCGTTCCCTACATTGCAGGTCTTTATAGCAAAGTGTTATTTTAGCCCTACCT
TGAGATATGGTCAGATGTTAACCATGAATGAGAACTGAAATAGTGTGAAGTTGGGGAGAAAGCACCTGAG
CAAGACTATGTCCACCCAAGCGTTCATTTATGGCAGAAAAGTAGGAGATAACAGTTATCATTACTGTACA
AATTAAAGTAATTTTCTACCAAGGGCCAGGTAATAAGTAGCACATACTTGGAAAGTCTTATGGTTTTTCT
TGTAATTACATCAATGTCCACGATAGCTTGAAAATGGCGGCAGATGATGAATGGGGCTGCATCTCAGTTC
AGCTTTGTCATCAAAATAGGTGGCTGGTGCCTCCTACTTACCAACTGAGAAAACAGATCTTCTGGCAAGC
AAATATTTGCTTTGCTGATGACATCTGGCTTCTATGTGTCCCTTACTCCAGCTTCATTTAAACTTTACTT
GTCTTGTAACTCATAGTGGCAGTTTTTCAGTCATTGGCAGTCTTAGAGCTGCCCTTGGAAGGCGTTAGTG
GAACTCTACAGGAATCTCATCCAGTTCCCATCAGAACGAGCTGTTATAAGAATGCCAACCCACATTACTC
TCTCACCATGTGCTCTCTCCTTCTTGTGTGTACCTTCACCATGGGGAAGCCACATATACCCCAACGGCAC
TGCGACCAAGTGACACATTAATGAGGTCACTTGGTGTTGTCCAACACTGTGCATTAAATATGCCTCTTTC
CTTTAGAGGTGTCCAGCTTCAGGGATGTTAAATCTTACAGTTTTTCACAAGATAACAACAAATGATCAGT
TAAGATGTTTAATATTTTTTATATGTCATCTCTATAAAGATATGCAGGCCTGCACACATAATTTCAATAT
TAACATGGAATCCTCTTTCTTAGAGATCTGTGCAGTTCACTCTTGGTTATCAACTTGATTAACCTGCAAT
CAGTTAAGAAAAATGCCTATGGAAGGATCTGGGTGGTTGTTTCCTTTAAGAAGTGGGGAAGGTAGACCAG
CATATATCCTTGGGGTGGCCTGAGGGAAAGCTGCTTTTTGCCTGCTTCCCTTCTCCCCCTGCTGGTGAAC
ACATCTACTCTACTGTTGCCATTGCTGCTGTCATCCTTCAATGACATTGGATGCGGCTTCTTCAGTTTTC
CAAAATGGACTCAAAAGGAACACTCCAGGCCTTGATTATTAGATCAGCACTTCAGAGGCATGTCGGTCTG
AGCAGTTACCTCTCAGTGTGCAGGGTCCATTGAACTCCCTACTCCTAACTGTGTAAGCCAAGCTGGTAAC
TCTCCTTTGTAATAGTGAGTCATTCCATCAGTTCTGTTCTGTACAGAAATCAGATGACGCTAGATCCCAA
ACACTGCAGAAATTTAGGCCCATGGACCGTATTCCCTGTGTGCTACTACCTAGGCATGTTCCCAAGCATC
TCAGAGGAAAGACCTGTCTCCCAGCCCTCTGCCTCATCTGGTCTTCTACCATTTTCCTCCCTCTTCTCAG
GTATAATCACTCTCACAGTGGATTAAATTTGTTATGAAGAAAACAATAAACTGCATACAATATACAAATT
ACGTCTGAATAAGCATGCAATAAAATTTCATAGGCAGAGGGAGAAGCAACTTTATCATGGCACAAGAATA
ATAAGCAATATCAATACATGTTTCCAAAATAGAGGACGCTTTGGAGTCAAGTGGCCATATGAGGGAATAA
ACTTTGCACAGAAGCAAAGAGGTTATTGCGGGAAACCACTGCACCGTGAGACTCCTTGAATGATGCCACT
CTCTGAAGAGTCACTGGTGGAAGTCAAGAGATACAGTTGTAGAAGTGTGGTGCCAGGTTGCCTGTGACCT
TCACAAACAACCTGCAGACTCTGACTGAGTATAGTAGGCAGGAAAAACCACGGGATCCTTAAACAGTGAT
GCTGAGCAACAGTTGCACATGATAGGAATTCAATGCGGTATTTTAATAGTTCAACTATAAACACAGACCA
GAAGTTTACTTCTCAGACAAACTGTGGCTCTAAAGCAACGAACAGCCCTTGTACTTGAATGAATTGTTTT
ACAACTCCTGGCTATCCATGCTCACTCTTCAGGGAAGGAGAACCTGTCAGTTCTGAAAACGAGTTTAGCT
GAATGCATGTGGAGGTTACAAGTTAATGATTAACTAGTAAAGTACAGGGCTGTTCTCTAACAACTAAGAC
CCTTACATGCCCTCTCTCCATTTCTCCAACACACAGACCCCACAGAACCTCTACTGGGATGGCTTTTGAT
TGTTGCCTTCTTGTCTGAATTTCCAAACATCTCCTGCATCCTATTTTCCTGCACTTTAACCTCCACACTG
AAGCTAAACATTAATCTCTCCACAAACCTATTTTCGGTAATTTAGATATCTCAGCAGAGATTGAAATAGG
AAAGATTTGTCTGAAAATTTCTTATAAGCCCTCTCCTCCTAAAGATCTCTTTTGTGTCTCCACCATTACC
TTCGTATTTTTATAAAACTATTACTGTAAGAACCAAACAAGGAAAACCTGGAATGGGTTCCTACATGTTC
TTGTTCCTGCAGACTGATAGCAAGACCCCTCATTCTTGATACCAAGCCCTACTGATAGTGATACCCAAGT
TTCTTGTATATTAGGGGAGAATACTTGCTCATATTTTAAAACATCTTTAAATTACCCATACTAACAAATT
CAGTATAAATGCTATAGAAATGAATACACTAATGGTCAAGAAAAAATAACAAAGAAAAAAATCTGTGCAT
GGCCAGTGTGCTAGTTTGAATATGCTTGGCCTAGGGAGTGGCACTATTAGTGGCTGTGGGCTTGTTGGAA
TAGGTGTGGCCTTATTGGAAGAAGTGTGTCACTGTGGGCGTGGGCTTTAAGATCTTCATCCTAGATGCCT
GGAAGTTAGTCTTCTCCTGTTTGCCTTCAGATGAAAATGTAGAACTCTCAGCTCCTGCTGCACCATGTCT
GCCTGGATGCTGCCATGCTCCCACCTTGATGATACTGGTCTGAACCTCTGAACCTGCAAGCCAGGCCCAG
TTAAATGTCCTTAGAAGAGTTGCCTTGGTTATCATGTCTTTCACAGCAGTAAGACCCTAACTAAGACAGT
CAAATTTTTCTGCCAAATCTATTTGATCTGTGATTATTGAATCATGGCGTTGAAAGGGCCAACATCCCAT
AGCAGGTGGGAAGTGAGCCAGAAGTTCTTTAACTACTTGAGTAACTCTTTCCAGCTTCCTACAGGGATAA
ACAACCAAGAACAGAGAGAATCTGTACTCCACACTAACAACAGTAGCTACACCACATTGTCCAAACCCCT
AACTTCTCAGAACTTTCCAGTATTGACTTTGCACTCTGAATGGACCACTGTGCAGAAGAGAGGAGTCAGA
CCCAGAACCTTATAGAGCCTCTATATGAAGCTGCACAACCTGTCAATGAGTCCTGATTCCAAAAAAGATA
CTTCTGAGAAATATGTTCTAGCTAGAAATATGTGATTACGATGGCTTTGATCAAATGTTTGATGGGGAAA
AGATAATTCTTGATAAAAAGAAATTACGTGGAGAACTCAATTACCAATATTTTCCATAGTTATACTGTCT
AACAAGGCTGGAGAGACGGCTCAGCAGTTAAGAGCACTGGTTGTTCTTCTAGGGGTCCTGAGTTCAATTT
TCAGCAACCACATGGTGACTCACAACCATCTATAATGGAATCTGATTCCCTCTTTTGGCACACAGGTGTA
CATGTAGATAGAGCACTCATCTACATTAAAACAAACAAACAAACAAACAAACAAACAAATCCTGGGGCTT
AGATTGTGTAATGAAAAAACTGACTCTAAGTTGTTCTCTGGCCCACAGTGACACACTTCTTCCAACAAGG
CCATACCTACTCCACAGCTCCATAAGCTCCTGGGATACATATCCCATGGGCACATAAATAAATAAATATT
GTCTAACAAAACTAGAAAAATTACCATTGTTAAATATTTCCCAGATTTTACATTTTCTTTTAAAAATCAT
ATTATAATTGAAAATTTTTACTTTAAATTATATACAAGCATACATGAATGCAGTACCTGAAGAGTCCAGA
AGATGGCATCAGATCCTGCAAAATTTAAGTTAAGGATGGTTATGAGTCACCTGACCTGGGTGTAGGGACC
CGGATGTGGGTACTCTGCAAGAGCAGCAAGCACTCTCAGCTTCTGAGTCATCTCTCCAGCTTCCTCATTC
CCCAAGACACACATCAAAATATTAGAAGTACATAAGCAAACAGCAAATCTGAGTATTGGTTTATTAGTAA
CTTATATGTGGTGGTAATAAATAATCAGTTTTACTTTTGTTGGCATCTGAACAAATATTCAGACAACTAC
AAGTTAGCAAAGTGTTTTTGAAAGGGAAAGTACACCCAAGAGCAAAGTCCCACATTAATAACCTCTTCCA
TACAGAAGAGCAGTTTTATTGTTCCAATGCTGTTTAGAGCTGGAAAAAAAATGTCTGCAAGACTCATATC
TTATTTGATTTTACTTCAATAAATTTTTATATTAAATTGAAGTTTGTGGACAGTACACTGCCAGCAGACA
ACAATGACACTTGCCCTCAGCATCCACACAACTGATCGATGTTGGAGAATGGTTCTCTGCCTATAGAGAA
ATCTCCTTGCACTAACAGCCCTCAGTCAAGCTCCAAATGACTTGGCTGGAGCGTGCATGGAAACACTGCA
CGCATGAACAGCAAGAGTCTTCTGGAACAGTTATGGACCACTCAAGTGCTTGTCTTGTCTTGGGAGAAGT
GATTTCGTAGCTTCCAATAGAGACAGACAACATAAAACTTATAAAAGGGTAAAAGACGTGGTACGAATCT
GGTTGCCTCAGCTTAGCCCTTCAGTCACTCAGGACTGTGCCTGACTGAAATCTTATCAAGCAAATGACAA
GAGGAGAAATGAAAATGTGTTTCCTGGCCATAGATAGCATTTGAGTGTCAGCACTGCTCATGGCATGGAT
GTACAAGGAAACAGCTTGTTGGGGTCACTAATTACTCTTGACTTATTTTCTTATTTTTAAAGAGAAATGC
CTCTGTTGTTGGGTTTCCTTTTGAACAGCAAGTGTCTTGGATGGTTTTATGTCACCTTGACATAAGCTAG
GATTATCAGAGAGGAGGGAACCTCAGCTAAGCAAATACCTCCATAAGATCAGAATGTAAGCAAACCTGTA
GAGCATTTTCTTAATGTGTGATTGATGGAGGAGGCCCCAGCCCAGTGTGAGAGGTGCCACCCATAGACTG
GTGAACCATCGACTGTGTTCAATGAGAACACAGGATGAGCAAGCCATTAGCAGACCTCTTCTACGGCCTC
TACATCAGTTCCCACCTTCAGGTTCCTGCCTGGCTTTGAGCTCCTGCCTTGGTTTCCCTCTGTGGACTGT
GATTCGGGATATGTGAGCCAAAGAAACCCTTTCCTCTAAGAGTTTCTTTTGTTCATGATGTTTCATCCCA
GAAAAAATAACCATAACTAAGACACCAAGCAATCTGTTCCCAGACCAAATGAAAGATTGCTAAGAATTAT
GCTATCCTTTAAAAACGTTTCGGCTCCTTTTTGTAGAAGTTAATCAAACAGGCCAACTGCCAAGAAAAAC
TGAAAGGAATAAAATGTTTCATGACTCATTTTATAGAAGAATTTAAAGCATCAATACAAAACTGTTTGAA
GGAAGAAAAGTAAGGAGGGAGGGAGTAGGAGAGAGTAAGAAAGGAAGGTAAGCAGAGAGAGAGGGAAGGA
AGGAAAAAGGAAGGGAAGGAGGGAGAGCAGAGAGTAAGAGAGGAAGGAAGGTAGAAAGGAAGGGAGGGAA
GGAGAAAAGAAGGGAAGGAAGGAGAAAAGAAGGGAAGGAGGGAGGGAAGGAAGAAGTGAGGGAAAGAGGA
AGGGAGAGGAAAGAAGCAAAAAGGAGAGAGGAAGAAAGGATGGAAGGTAGGAAGAGAGGGAAGGAAGGTA
AAAGAAAGGAAAGGAGGGAGGGAGGAGGAGAGGAACTATGTTGTAAGGGATATAGACACTTGGTCATACA
TCATTCAATTAGTATATAACATAACCACTATGTTGCTGAACTGTAGGACATCAGGTGTGCAGTGGCTCGT
CCAGATATCACCAGTACCAGCATGTTAAGAGAGCAAAGCTGTGGCCATTCAAGGTCAAGAGAGGGTCTGT
GAGATAGATCTGGGAGTGAGGGTGGAGCATGACTTTGTGTGTGAGAGACAGGGAGTGACAATGCAAGCAA
GCCTGGGTCCGAGCTTAGGCATCTGGATCCACGTGCTTAACGGAACGGTTGTGGATTTTTTTTTTAAAGA
AAGAAAATATTGGAACAAGAATTTGTAACATGGCAGAATTGAAATGAAAATATATTTGCCACAGTGAAAT
AATCTCCAAACCACCTGATAGATATTGGGGACTGTTTCATAGGAAGCACGTCTCAGCAGCTAGGTCTGGA
GTCCAAGGGGACATGTTAAATGATACGTGGTTATTGTCATTTCAAATGTCCAAGTAAGACACCCAAAAAG
GCTTGGTTGGTTTCCACGTACAGGGAGGAGAGATGCATAGACTGTGTTGTTCTTTCCTACAAGAGGCTGG
TGGGGGAACAGTTCAGAGAGAGAACAGTGGGTGCTAAGATGAAGGCTATGTCCAAATACCCAAGTCAAAC
ACGCCCTCAGTAAAAATAGCATGGGGAGCTTTGTTAGTTCATCGGTAGCTAACAGGACGGCAACACATAA
AGATGCTTTCCGGCCAAATCTTATGAAAGCTTACTTTATAGGCAAGGCATAAAGCCATCTCCAGGAGGCA
GCTTTACGAGAGTGCTCTCCAGCAAGTGTCTGACAAAAGCAGCCATCTTACAAGTCTCAGATAGTTTGAA
CAAAAGAAACAAAACCAACCGGCAACATAATATTTACCCCATAAACGCAAGCTCAGAGACAGGGAGGGCA
CACTTCTGAGCATGGGACTGGTGGCCTGTCACTCCCTGTCTCTCACAAACAGAGTCATGCTCCTCCCTCC
AGCTACAATAAGGAACTGCTATCAAACTGGAAAACCAACACATCGGCTTCCAAAACACATGGCCTCCATG
GTCCCCCCTAACAGAACCCTACAGCTTTATGAAGCCACGCACTGACAGAACAGCAATGGAAACAGAAAAT
AAAATCAAAATGAAAACATAAACAGCTGTTTCCCTTGCACCGTGGCAACACTGCAAATATATAGCAGTAT
ACGTGCCTGCCTGGTGGAGGCTTCTTTTGAATTTTTTGGAATGCCAAGTGGCAAGGCAAATAGATTCACT
CTCTGTTGGTTGTTATTGTCCTAATTTTATTGCCAAGAAAAAACAATCTTTCCCTATGTGCATGTTACCC
TTAATATCCTCTCTTCCGGTGAGAGAAGTTATAGCTTAGGCCCAATTTATAATTTTACTGTTCATTTAAT
TTCCTTTAACACTTTAAAATGTTATGTGAAGGCTCTGACTTGTCAAAACTGAGCCATCCATTTCAGGCAT
AACCCATCAAAATGACAACACGAACAGTTCTCATAATCTACAACAGTGAAAAAGGCAGACCCAATGCTTT
AAAAGAGAGAGTTTGTCCACACCAACTACTCTGCACTATCATGTTTGACATTCGTGTGTTATTTTAATAA
TCTGTGCTGGAGAGCAGAAATATTTGTGCCTCATTTAAAATGCTATCAAGTATAAAGTTTTTTTGTTTTT
GTTTTTTAAGTAGCATTTGAGATAAATATATTTCTGGCCCAAAGAATAAAGAAAGATGTCTTTGAGAATA
CAAAAGCCTTTATTTCATAAGAAAAAAAATTATCTCTAAAGCTTCCCCACAGCCGGTCCTCCAGACTTTC
CACCAGGCTTTGCTTGAACATGGACGAAAGTACACACACCTGGAATTCCTTTTTCCTCTTTTTACAAGAT
CCAGGAAGTGAATTCTTATTAGTCAAACCCAACTCAAACACATCATAAATGATACTTGTATCATTTATGG
AATTTGGGAACTATGCATATTTATAAGCATGAATGCTGCAGCCCAGTGATGTCTATTGGCTTGCCCCTAG
TGAGTTATCAAGTAATCGAGCTTTAAAGCTGTTTAGCCCATAATCCACACCATTCAAAACACACAGAAAG
TGGTTACAGTCTGTCAATGGACTCTCTGGCCTGGTGAATACCAGGAGAAAAGGTGACTCCCTGGGTATTC
AGCATAGAGACTAAGAAGCTGTCAGTTAAGAAGGCAATGGTTCTATCAAAGTGTTATATTCTACTGCCCA
AGAGATGCGCGCCATCTTTATCCTATTTAAAATCAGTAGAACAACGCTTGTATCAGCTTCTCCTTTGTGG
GGACATTAAATCTGTTCATGTAAATGTTTGTGATCTCTAGTTAATTATCTGTGGGTATTTTATTATCCTG
GGTATAATTTCCCTTTTCTCTTCTATACCTTTGTTTTCTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
GTGTTTTAAGACAGGGTCTCATTCTGTAGCCATGCTAGCCTGTAACTCACTAAGAAGCTTAAACTAAGGG
CAACCCTCCTGACTCAGGTGCCCAAGTCCTTGGATTACAAGCATGAGCCATGATGCCTCCTTGGCTCTTT
CTCAGTTGATTTTGCATCTTTTGCAGCTCCCCTGATGCCTTATTATGAGACTCAAAAACATTTAACACAA
TTAACTGGGAAAAAAGGCAAGTAAGTAGAAAAACTGTAATTTCCATGGGTATTTGGACATGATGCTAGTT
AACCAAATTTCTTCAGTGGGTTTATGAGACAGAGGGGAGAAGGCAGCATCAAAGCAGGTGATAAAATAGG
GCAGATTACACTAACAAACACAAGCACAAATCAAAAATTATGTAGAGCATAACGAAGCATCATTTATGCA
CTGCTTGACCATCTGGACCTGCTGTTTTATGGGACAAGGGGGTGAGAACTTGTGCTTGAGCCTTCTGAGG
TGGTACTTTTTATAGCAGTATTTGAGACCCACACAGACTGGCTGTACAATGATATGGCTGCTTTACAGAA
GGCCCATCCATAGAAATCAAGCCCTGCAATGAATGCTGTGTAAGGACAAAGCCCCAGAGTTCAGTCGGCT
GCAGACTTGCTGACATTAATTCTGCCAATTCCTTGTGATTCATAGATTTATGCTTCTCCCTTCAAGTGGG
AGTATGACAGGGGGAGGACGCGTGTTGAGATATCGTCTCTGTGCTGTGGTGGGTCACTTAGGACTATGTT
GTGTCATACTGAATGGCTTACCAGAGAAGTTGCTGAGCACAAACATCAAATATGACATTAGTTATAAAGA
AGAGAGGAAGAGGGAGAGGGAGGGGTGGGGGAGGGGAGGGAGGGAGAGAGAGAGAGAGAGAGAGAGAGAG
AGAGAGAGAGAGGGTCGAAAAGGGGAAACGACTGACAGAAATTATGAGGAAAAATTAGACTTAAAATTAC
ATATACTATATAAATCACCAACAGTTTGAAAAGGATGAGAGCTATGAAACACAGCATCCATATTTCCAGC
ATGATAAGCAGCTTTTGCTGATAGCCAGTGAGGAAATATCAATCTCATTCCTAACCTACAAAGACTGAAT
TCTGACAACAAACTGTAATGAGCCAGGCAATGGATTCCTTCCAGCATCTCCAGATAAGAGCTTCCCCGGT
AAATGTCTTGCTTTCTACCTAATGAGACTCTGGAAAAAATATCCAATTGAACTTGACACCCATGTAAACT
GTGACTCTATTGAAGATGTTGTTTTAAGCCTCTTTGTTCATGACTTCTTACACGGCAATAGAAAGGTAAT
TTATAGAGCAATGTGTTTCACTGCAGGCATTGTGGAAAATAGATATAAACTAAATATTATGTTTATCTAT
TTTATAAATAAATATATACAATACATATATTATATAGTTGCCTTAAATGATTTTAATAGGAAGCAATGAG
ACTACATATGCAATCAATCTAGAGCAGGTTTTTGGGTAATAGTTATTTTTAACGTGTTTTTCTTTCATCT
TTGGGGTTATGTCATTTATCCTTTCCCATCCTCCCTCAGCCCTTCTATACACTCCTCCTGCTCTCTCTCA
AAGTTATGGCCTTTTTTCATTAATTGTCATTGCAGGCATACGGGGGGGGGGGAGGGAGGGAGGGGGAGAG
AGAGAGAGAGAGACAGAGAGACAGAGAGACAGACAGACACACACACACACAAACAAACACACAGATATAT
ATTACAAAATATAACCTACTCAGTCTGTATAATGTTACTTGTATGTATGTCTTCATTACTAACCATCTGG
TATTGGATAACCATTATTGTGCTCTTACCTGGAGAAGCCTATTGCTGCTACCCCAGCTTTCCCTATGGTG
ATGAGGAAATATATCAAAGGCTTAAGGGAAGACTTTGGTGACTATGGTTGCCTGATAGTCTAGGGAATTG
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTCAAAATTGTTCCCTTG
GTTGTATAACCTGAGAAGTGACCTTATGAGTGTTGCTCTCCAAAAGGCTATGTTATATATTATGAGGCAG
AACACTTCATAAATTGCATGTGCACACACACACACACACACACACACACACACACACAATCATTGCATGC
TTCTTGTTTTCAATGCCCAAATTCATCCGTACTGATAAGAAAGAGGGATTCTACAGTACCATGTGCTTAC
ATGTGAATATGTAAAAGAAGGAGGGTAACTTTACTACAATTTATTCCAGATAAAATTAAACATCAATTGC
CCACTGCAGTGCTCTAACAACAGTGGGAGGAAATGATACAGTCATGAACAAAGACAAGGTCATATTTGTC
TTCTCTGAGCTAGCGCTCTACAGAAGAGAGATGAGCCTAGCAACTGCAGATAGTAGTCTATATTAGAGGA
AGGTAATGTGCTAGCATATAGAGGAAGAGGTTTCGCTGGGGGAGGCTTTCTGCAAGAAGCAATGGGAGTG
GACCTAAGATCTTCAGGGCGAGAAAACACTTATTGAGAGGGTTTCAAGCAAAAGTAAACTAATCATTGGC
CTTGTTATAGGGGCAGGAAAGTGACAGCAACCTAGAAACAAAGTTTACTAATGCTTACTAACACTGAAAA
TGGTGTAATGATCAGGACTTTGAAATGCTCAACCTGCTGGACTTTCTCACAGGTGTAAACCCTTCCCTCA
TGACACACCAGACAGTAGCTAACCAAAAACTCGTGCCAGCAGCAACTGCAAGCAGACTGAGCTGAAACTT
GCTGGCCTGCTTTCCTTATTATTATCTACTACTTGTGGTTACGAAATAGGAAGAATACAATGGTTGACCA
TCACAAATAAAAATGAATTTACCCTTTACTACATGGTAAATTAAAACCTGCCCCACATGTATGTGGGCTA
AGACAGTCACAACAATCTTACTTATTTTGTGTGCCTTTCCAAAGTCCCTTTACTGATGTGGGGTTTCTAC
TTTTCTCCTCAAATCAGCACCAAGTCCTGACCATACATGCGTAGTTATGCCTGAAAATATGTGTGAGTTC
CACGGCTAGGACTTAAAAGGTCTGTCCTCTCTGTTCCTTCTACTGGCACACAAGAAAACTCAGCGAGACA
ATAATGATTGAAAACAGTATAGACGTTTATACCTGCCATCCCAAGACACGGAAATGAAAATAAATCAAGG
AATCAATCGATAAAACTATGAGGAATAACAAAGTATATCTAAAATAACTCTATGTGACCTAAACTGAGCA
TCTATAGATACAAAACTATGTACTATAAATGTTATTAATTTATCTGAAATAAAATATTGTCTTTAAAAAC
TTCTGGGTTGTAATTGGGGAAATTTTGATCTCTATAAATATCTCTTGAAAATAAACCTATCCTGGCTAGC
CTTATAATTAATAAAATCATGCCAACAAAATATATTTAATCTCTAATATCTATGTGATCAAGGGAACAGG
ATGGACTCATTATTCATGGCTAGTTTAAGAAAGTCCTTGGTTAGAATGGACAGAATACCAGAACTCAGAT
TTTCTTCATTTGAATATTATCTAAGATGTATTTGCTTTGGTCAATATTTAAAGAATGTAACAATTTCACT
TTCCATATGCCAAATCATCACAAAGTGTTGGTGTCAAACTGTTTCCTAAAGCTAGTATAATTAATTTTGT
CTTATACTATAAAACTCTTGTAAATCAAAGGTATCACTCTTTGTTCTTGCAATATCTAGGAAGTCAAGAA
TTATTCATAATTACTTCCATGGACCTATCAAATTAGGAATTTTCTGATATAATTTTGTTAAAATAAAATC
AACATTTAAAAAGTCTGGATTTTGTTTAACTTACTTTATAGTTGATTGACAGACGCCTTTAAAAGATGCT
TCAAGGTGCTGACAAGTTAAGGATAATTGAGAGTCTTCTTCTGGACAGCCTTGTGAGAGAAGCTAGCAGT
GGCTATTGAATGCTCATCCCTGAATGGAACATCTACATTACACCCACCAAGGCCCACGATGGTAACCAAG
AAAGGATGGAAAGGCTGTAAAAGCCAGATGCAAGGAAGGAATGCTGAGAAGAGTGGTGTCTTCTGGACAT
GGTCTGGCTGTCACTGTCATGGACACATAGTGTCTGTGGCTATGTGCTCAAGAAAAACATGAAAGCAGAA
ACATGGATGGTTGAAAAGAAAAAGGCCTGTGTGTGAGTAAGAAAGGCATTAGAGAATGTATTGGGGAATG
AATATGATCACAATAAATCGATGGTGTGCATGTAATTGTCAAAATGAATAAAATTTTCTTTCAACTTACA
AATATTTATATACTTTAGTGATAACAGTCAATTTTAATATTTTCATGCTATAATTCAACTTCTGTTACTG
TGTGGTTACAGCAAAGATCATTTTCTCAGTCCCAACATACAGGTATTTCTTCCCATCCCTGAAACCAAGC
AACTCCACAGGAGACACCAGTTAAGTATTTGATAATTCATTATACGCCTAAGGTTGCCTCTTTGAGAAAG
TGTCAGACCTGGTGTATTGGTGGCTTTGTTCCACAACTGTACCCCACTTCTGACACCTGAGACACGTCTG
CCCTCTGGAATTCCTGCTGGACTGCCTGCAGTTAGAGCCCAGCATCCTTTGTTAGCCCTATTAAATTGCT
ACAGCATTATTCAAGATGCCATGGGAACACATTTACTAGTTTGTTGAGAAGGATATTTTTAAAGAACAAA
CAACCATTTGAGCTACTATGATAAGGCCTGGTAGGGGCCAGACACAGGAGCTTCCATCCCTGTGTAACTG
ATATGTGCAACTCTCCTGGAGTGAGAACAATTCTCAGCTTGCACATGTTCTAGAAAGTTCTCTGAACCCA
TCTCTATGGCTATTTTAAAGGAGATTTGTTTACATAAGCAAGTCTGGAAAACAGGAACAGCATGGAAATT
GCTAACATACTAGATGAGACCTACCATACTAGGTGAGACCTACCTACGCTGTTGCACCCAAGGCTTCTTG
TCCTGCTGAGTAATACTCTTCCTGTTGGCTAACTCACAAGATTCTTGCTAAAATGAGATTTTGTGAATTA
CAATCAAGTAAAGCAGGCAAGAGACCTCCTCTAAGACCAACTTCAAGAAGACAGTGGAATAGAGAAGCTG
GAAGCTGTTTTTAGTTGCCATGATGTGAATTGGGGAAGAGGAAAGAGGTGAGACTATCTTTAGATCTATG
ATTGCCATTTAGGAAGAGAGAAGGGAATGGGGAAAGGAAGGGCAGGACATGTTAGGGAGCAGTTGCAGTC
AGAAGCACTCCACCAGCAGAGCAGCCAAACCTGCCTACATTGCCTCCACATGGCAGCAAACATCAATGCC
AAGTTATCTAGTAACCACAGTAACCTCACTTTTTAATTCTCCTCTGTACAGAGACTAATCTAGATCCTGA
GATGTACCATCTCAGTCAAGGACCTGATCTCCTACAGTGGATACAGTAGATGACAAATCGTGGTTTCCTG
TGCCAAATTAGGTCAAGGTCATTTACCTTGTTTTGTTCCACATTCTTATTTCTGAATTACACTTAAGTAT
AAAATTACATCATATATATATATATATACTTATATTTTAAATATAAAATCACAATATTTAATGTTAACAT
ATGTATTAAATATGCTACTTTTACTTTTAAATATATATATAATTTATTTTAAACTATATATAATGTATTT
TACTTTAAATTATACTCACAACTTAAAAAGTAGTACATATATATATATATATATATATATATACACACAC
ACACACATACATATATATTAAAATGTATACATTAAATGTGAATATGAAGAATGACTCTTTAAGGTGACTT
TCTAAGGCAGGTCCACTCAGGAGTGCACAGGCTACAGAAGCAGAAAAACTTCTGGAACAGGGTCCTACAG
TGTTGCTCTGCACCTGGGAGATGGGGCTGTTCCGCAGCCCTCTGTATACTGGTCTTGTAGGGAGAGAGCT
GGTCTCCTAAGAGTGCTGACACGGGCTTAAAGACTCACAGGAGGAACAAGCTCCAGGCAGAGACAGCAAG
AACATCTAACACCAGAGATTACCAGATGGTGAAAGGCAAACACAAGAATATTACCAACAGAAATTAAGAC
TACTTGGCATCATTAGAACCCAGTACTCCCAACAAAGCAAGTCCTCGATGCCCCAACACACCAGAAAAAC
AAGATTTAGGCTTAAAATCATACCTCATGGTGTTGGTAGAGGATTTTAAGAAGGACATTAATAACTCCCT
TAAAGAAATACAGGAGAACACAACTAAACAGGTAGAAGCTCTTAAAGAGGAAACACAAAAGTCTCTTAAA
GAATTACAGGAAAACACAACTAAACAGGTGAAGGAATTGAACAAAACCATCCAAGATCTAAACATGGAAG
TAGAAACAATAAAGAAATCACAAAGGGAGACATCTCTGGAGATAGAAAACCTAGGAAAGAAATCAGGAGC
CATAGATCACCAACAGAATACAAGAGATGGAAGAGAGAATCTCAGATGCTGAAAGTTCCATAGAAAACGT
TGACACAACCATCAATGAAAATGCAAAATGATCCTAACTCAAAACATCCGGGAAAGCCAGGACACAATGA
GAAGAACAAACCTAAGGATAATAGGTTTAGATGAGAAGGAAGAATTTCACTTTAAAGGGCCAGCAAATAT
CTTCAACAAAATTATAAAAGAAAACTTTCCTAACCTAAAGAAAGAAATGCTCATGAACATACAAAAAGCC
TACAGAACTCCAAATAGACTAGAACAGAAAAGAAATTCTTTCTGACACATAATAATGAAAACACCAAATG
CACTAAACAAAGATAGAAAGATAGCAGTAAGGGAAAAGGGTCAAGTAACATATAAAGGCAGGCCTATTAG
AATTGCACCAGACTTCTCACCAGAGACTCTGAGAGCCAGAAGATCCTGGACAGATCTCATACAGACACTA
AGAGACCACAAATGCCAGCCTAGTCTACAATATCCAGCAAAACTCTCAATTATCAAAGATGGAGAAACCA
AAGCATCCCATGACAAAACCAAATTTACAAAATAACTTTCCACAAATCCAGCCATTCAAAGGATAATAAA
GGGAAAATTCCAACACAAGGAGGGAAACTATGCCCTAGAGAAAGCAAGAAAGTAATCTTTCAACAAACCT
AAAAGAAGATAGACACATGAACAGAATCCCAAATGTAACAACAAAATTAACAGCAAGCAACAATTACTTT
TCCTTAATATCTCTTAATATCCATGGACTCAATTCCCCAATAAAAAGACATAGAATAACAGATTGGCTAT
GTAAACTGGACCCAACATTTTACTGCATACAGGAAACCCACCTCAGAGACAAAGACAGATACAACCTCAG
AGTAAAAGGCTGGAAAACAATTTTCCAAGCAAATGGTCCCAAGAAACAAGCTGGAGTAGCCATTCTAATA
TCGAATAAAATCAATTTTCAACCTAAAGTTATCAAAAAAGACAAGGAGGAACACTTCATGCTGATCAAAG
GTAAAATCTACCAAGACGAACTCTCAATTCTGAACATCTGAGCTCCAAATGCAAGGGCATCCAAATTCAT
TAAAGAAACTTTAGTAAAACTCAAAGCACACAGTGCACCACACACAATAATAGCAGGAGACTTCCACACC
CCACTCTCATAAATGGATAAATCATGGAAACAGAAACTAAACAGAGACACAATGAAACTAACAGAAGTTA
TGAACCAAAGGGATTTAATAGATATCTATAGAACATTTTATCCTAATACAAAAGGATATACCTTCTTCTC
AGCACCTCATGGCACCATCTCCAAAACTGACCATATACTCAGTCAAAATACAGGCCTCAACAAATATAAA
AATGTTGAAGTAATCCCATGTATCCTATGAGATAACCACAGACTAAGGTGATCTTCAATAACAACATAAA
TATTAAAAAGCCTCCATACACATGAAAGCTGAACAACACTCTACTCAATGATAACTTGGTCAAGGAAGAA
ATAAAGAAATTAAAGAATTTTTATAGTTTGATGAAAATGAAGCCACAACATAACCAAACGTAAGGGACAC
AATGAAAGCATTCATGAGAGGAAAACTCATAACTCTGAATAACTTTAAAAAGAATCTGGGGTGAGCATAC
AATAGCAGCTTGACAGCATACCTAAAAGCTCTAGAACAAAAGGAAGCAAATTTACCCAAGAGGAGTAGAT
AGATAAACCCTTAGCTAGACTAGAGGGCACAGGGAGAGTGTTCTAATTAATACAATCAGATATGAAAATG
GAGACATAACAATAGAATCTGAGGAAATAAAAAAATCATCAGATCCTACTACAAAAGCCTATACTCAACA
AAACTGGAAAACCTGGATAAAATGCACAATTTTCTAGACAGATACCAGTTGCCAAAGTTAAATTAGGACC
AGATTAGTGATCTAAACAGTCCTATGTCCTCTAAAGAAATAGAGATGGTCATTAATAGTCTCCCAAACAA
AAAAAGCCCAGGACCAAAGCAGTTTAGTACAGAGTACTATCAAACCTTCAAAGGAGAGCTAATTCCAATA
CTTCTCAAACTATTCCACAAAATAGACACAGAAGGTACTCTACCCATTCATTCAAAGAAGCTACAATTAC
TCTGATACCTAAACCACACAAAGACCCAACAAAGGACAACTTCAGACCAATTTCCCTTATGAATATTGAC
GCAGAAATACTCAATAAAATTCTCACAAACCAAAACCAGAACACATCAAAACAATCATTCATCATGATCA
AGTAGGCTTCATCCCAGGGATACAGGGATGGCTCAACCCACTATATAAACAAACTCAAAGAAAAATTCCA
CATGATCATCTCATTAGAGGCCGAGAAAGCATTTAACATAAGCCAACACCTGTTCATGATAAAAGTCATG
GATAGGTCAGGAATTCAAGGCATATACCGAAACATAATAAAATCAATATACTGCAATCCAGTAGCCAACA
TCAAACTAAATGGAGAGAAACTTGAAGCAATCCCACTAAAATCAGGGATTGGACAAGTCTGCCCACTCTC
TCTGTACCTATTCAATATAGTACTTGAAGTCCTAGCCAAAGCAATTAGACAATGAAAGGCGATCAAAGGG
ATATAAATTGGAAAGGAAGAAATCAAAATATCACTATTTACAGATGACATGATAGTATATATTAGACACC
CCCAAAATTCCATATGAGAACTGCTAATCCTGATAAACAAATTTAGTGACATATATGGATATAACTCAAA
CAAATCAGTAGTCGTTCTCTACACAAAGGATAAGGCTGAGAAAAGAAATTAGAGAAACAACATCTTTTAC
AATAGTCACAAATAATATAAAATACCTTAGTGTGACTCTAACTAAGCAAGTGAAAGGTCTGTATGATAAG
AACTTCAAGTCTCTGAAGGAAGAAATCAAAGAAGATCTCAGAAGATGGTAAGATCTCCCATGCTCATGAA
TTGGCAGGATTAATATAGTCAAAATGTATATCTTACTAAAAGCAATCTACAGATTCAATGCAATCACCAT
CAAAATTCCAACTCATTTCTTCAGATTTAGAAAGGGCAATTTGCAAGTTCATCTGGAATAACAAAAAACC
TAGGACAGCAAAACTATTCTCAACAATAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCTGGTGGAATCAC
CATCCCTGACCTCGAGCTGTACTACAGAGCAATTGTGATAAAAACTGCATGGTACTGGTACAGTGACAGA
CAGGTAGATCAATGGAATAGAATTAAAGACCCAGAAATGGACCCATATACCTATGGTCACTTGATCTTTG
ACAAAGGAGCTAAAACCATCCAGTAGAAAAAAGACAGCCTTTTCAACAAATGGTGCTGGCTCAACTGGCA
GTTATCATGTAGAAGAATTCAAATTGATCCATTCTTATCTCCTGTACAAAGCTCAAGTCTAAGTGGATCA
AGGACCTCCACATAAAACCAGAGACACTGAAACTTATAGAGGAGAAAGTGTGGAAGAGCCTCGAACATAT
GGGCACATAAGAAAAATTCCTGAACAGAACACCAATGGCTTGTGCTGTAAGATCAATAATTAACAAATGG
GACCTCATAAAATTACAAAACTTCTCTAAGGCAACGGACACTGTCAACAAGACAAAAAGGCCAACAAAAG
ATTGGGAAAGGATCTTTACCAATCCTACATCAGATAGGGGAGTATTATCCAATATACACAAAGATCTCAA
GAATTTAGACTCCAGAAAGCCAAATAACCCTATTAAAAATGTGGTACAGAGCTAAACAAAGAATTCTCAA
CTGAGGAATACTAAATGGCTAAAACTGCTCAATATCTTTAATCATCAGGGAAATGCAAATCAAAACAAAC
CTGAGATTCCACCTCACACCAGTCAGAATGGTTAAGATCAAAAACTCAGGTGACAGTAGATGCTGGCCTG
GATGCGGGGAAAGGGGAACACTCCTCCATTGCTGGTGGGATTGCCAGCTGGTACAACCTCTCTGGAAATC
ATTTTGGCAATTCCTCAGAAAATTGAACATAGTTCTACTGGAAGATCCAGGAATACCACTCCTGGGCATG
TACCCAGAAGATGCCCCAACCTGTAATAAGGACACATCCTCCACTATGTTCATAGCAGCCTTATTTACAA
TAGTCAGAAATTGGAAAGAACCCAGATGTCCCTCAACAGAGGAATGAATACAGAAAATCTGGTACATATA
CCTATTGGAGTACTATGCAGCTATTTAAAACAATGAATTTATAAAATTTATAGACAAATGGATGGATCTG
GAGGATATCATCCTAAGTGAGGTAACCCAGTCACAAAAGAACACACATGATATGAAATCACTGATAAGTG
GATATTAGCCCAGAAGCTCAGAATACCCAAGATACAATTTGCAAACACATGAAACTCAAAAAGAAGGAAG
ACCAAAGTGTGGATATTTCGATCCTTCTTAGAAAGGGAAACAAAAAAGGGAAGGAATTGCGGAGACAAAG
TTCGGAGCAGAGACTGAAGGAATGACCATCTAGAGACTGCCCTACCTGTGTACCATAAACAACCATCAAA
CCCAGGAACTATTGCAGATGTCAACAAGATTTTGCTGACAGGAGCCTGATATAGCTGTCTTCTGAGAGGC
TCTGCCAGTGCCTGGCAAATACAGAAGTGGATGCTCACAGTCATCCATTGGATGCAGCACCGGGTTCCCA
ATAAGGAGCTAGAGAAAGTACCCAAGGATCTGAAGAGGTCTGCAACCCTATAGGAGGAACAACAATATGA
ACTAACCAGTACCCCCTCCCCCCAAGCTCCCTGGGACTAAACCACCAATCAAAGGAAACACAAGGTAGGA
CTCATGGCTCTTAGCTGCATATGTACCAAAGGATGGCTCATCAATGGGAGGCGATTCCCTTGGTCCTGTG
AAGGTTCTATGCCACAGTATAGGAGAATGCCAGGGCCAGGAATCAGGGTTGGAGTGCAGGGGGCATGGGA
GGGGATAGGGGAATTTTGGAGGGAAAACTAGGAAAGGGAATAACATTTGAAATGTAAATAAAGAAAATAC
CTAATAAAAAAATTAAAATAAAAAAGAATGAAAAACAAAACACAAAGCACATATATTAAATCTGCAAACA
TTAAATATTACTCTCTGATCCTTTACTTCCAAAAGGTTTGTAATTTCCTGACTTTGAAAGAATTCTAATT
GGATAACCCACTACTATCTCAAATACAATAAATCTAAAGTAAAAATATTTTCTCAGATACAGCACTCTCA
TCCTGCAACTCTTCATCTCTCCTAAAGCCAACAGAGTACAGGAATTCATCACCCATAAGTACCCTAGGCT
GGAGACTATTTGCTCTCAGATCAACTATTCTTACCTATCTTGTCACCATGATACTGGAAGCCACACAGCC
TGAACTGAGCACATCTTTCCATTGGATCCATGCAACTGTCTGTTGCTCATACACATGCTTATGCTGTGTG
CTCCTGGTTGTGGGTTTCAATCAGCATCTCTTAGTTAAGTAATGACAGTAATATGTTCAGGTCTTCTTGC
TAATCTTTGATTCTAATGCACTTTCAAAGTCTTGCTCAATTCTGCAACTTATTTCCAGCTTCATTTCCAT
TTAGTTTCCCTCAGGAAGACACTTGTGCCCTTCACTTTGGATTTAAGAATGATGTAGCCATATCCTCTTT
GCATTTTGCATCTTCAAATATAAAAATCAGAGTATTTTGCTATTACTGATGACATGAATAATAGGTAAGC
ATTATCAAGACTTCTAATCTTATGTTTATATGTTACTAATTTCTGAATCACAGCAACATTGATAGGAAAC
ATTATCATCATGTTAAATGTAATAAAGCTTCAGTACAAAGAAGTTGCATGTTGTCTTATACGATTGACAT
GATAAAATGCCATAACCCAGACAACTTGGGAAAGAGAGGGTTTATTTTGCTTACACTTCCACACCACAAT
TCATCACTGAAGAAAGCAGGACAAGAACTCAAAATGGGCAGGGACCTAAAGACAGGAGCTGATGCAGAGG
TCATGGAGGGGTGCTGCTTAATGGCTTGCTCCCTATGCCTTGCTCAGCCTGCCTTCTAAGAGAACCCAGG
ACCACCAGTTCAGGAGTGAACTCACCCACAATGAGCTGAGCCATCCCCCATCAGTCACTAAGAAAATGTC
CTACAGGCTTCCCTACAGCCCTATTTTATGGAGGCATCCTTTCAATCAAGAATCTCTTCTCTCAGATGGC
CTTACCTTGTTGACACAAGGTATCTTACTGCACAAGTGCCCAGATGATAGCAGCACCAGAACAGAAAGTC
AGGAAGCCTCTTTTGAGAATTAAGCCAGGGGTATCTCCAATGTTAACTGTCCCCACTACTTGGCTTCTTA
AGGATGCCACCTTGGAGATGAAATGTGGTCTAAATCTGGTTTCTCCATACGAAGAGAAGAAATTCAATCG
GTGAAGACTCACTATGAGGATGACCTACATTCTGATATTCTTCCAGGAGAGAGGGACAACAAATTAAAAC
AAAGCAGAAGCCACCATGCATGGACTGACTTCTCATTCCAGGGCTGGTACCCATGGGAAGGGAGTAGAAC
ATCATTGGTAGTGAACTGAGTACGCTGTATCCAGAATTACCAACACAAAAGCCATCCCAGTCTGGAAGCA
ACCATGTGACAGTGTGATAAGGATGAGAGTTAGTCAGGCAAAGAAGGACTGAGTTAACAGACACAAACCT
CCTCTCTGGAAGATAAAGAGATTTCTGCCAAAACTCACAGGGAACTTGAAAGAGAGCTGAGGCGTTTGTA
CTGGAGCAGCAGTTGCAAGATTGCCTAAAGGAAGCTGAACCCAGCCAGCATGTCAGAGAAAATGTATCCA
TGTAGCTTGGGAGACAGACAGGGTCAGGAGAGCACCAGTGTGTCCTCACATAAAGCCCTCCATTCAGAAG
ACAAGGGTATGCATATGTTCTAGGTTTGTGTGACATTAATGCAGAAGCAATCATTAATAAAATTACAAAA
TGTTGTCAACACAATTATATAATATGTATTAATAAAGAGAATTTGTTCCCTCTACAATCTTTCTGTGCTC
AAATTATATTTCTGGTACAATGGCTTTACTAACTATGACAATAGACAGAGCTTAATGTCTCATAATAAAA
ATGTTAACAATGCCAGACTATAGAATATTAACATAGCCCGTAGTACCAGAAAATTTAAATGAAGAGATTA
AATAAATAAGTTCATTAAGGGTTGATGGTAATAATAGTTCTACATGTCCATACAGATCTGAGTAAACTCC
AGAGTGTTCTAAGAGTGTGTTCTATGCATTTTAAGAATAAGAGACAAATGAACACACTCACATTATCAAG
CACTCCTAGAGAATTTAACAAAACGGACCCAGTGTTTCAAAAACTCCCTGTTTGATTAAATTCCACCCTT
CGTTCCTCCAACAGAAGAAACAGAGCCCTCAAAAGCAGAAGACCATCTTGTTCTTTCATTTTTTTTCCTG
ACTGAACACAATGCATCCTCTTTTAAAAGACACATATCCTGTGAGAACAGAGCAGGAGTAAGGTAAATCA
TGGATGGAGAGATGGAAGTTCTCACGGTGAGCCACTGTCAAAGTTCCAGGCAGGCTTGCTCTCACTCTTC
ATCCCTTTTGCATTGCCCCATAGATAAGCACAGGTATCTCAAGAGATAATAATACATACACCATGGCTTT
TCTTTTTAAGCATCTGCTACAAGTGCCATACAAGCTGTGTTACAGGCTATGGATCAGTCACGTGCCTAGG
ATATTTATGAGGCACATCTAATAATAGTCTCCATTTTACAAGTAAAAATAAGAGCACAGGAAGCTTGTGT
GAGGTTGTAATAAAACCACTAAATGGCACGTCTGGGATCTAAATTCCACTCCATTCAATACTAAACACAT
AGAGGTTTATGCAAATATAGCCACCACTATTTATAAGGAGAAAACACAATAGCCATGACTTCTGAAATGA
GGCAATTGATAATGCTCAACAAACACCAGAGGTGCCACAAAGATTGAAGTGTCAACATCTATGATATGTT
GGGGGAAATGTCAATAATAAATAAAAGCTACATAACCAACATCTTTCTTATGTATTGTTCTATACCCCAG
CACTATGCAGGAGAAGTAGGTTATAGAGTTTTAGGAAAGAGTATAATAATGAACACACTGGCATTACATA
GAGAAAACAAGTGTAGATTGGAAGACAGGTATGCAGTTAAAAAATTTCAGGTGTGGACTTAATCCTTAAA
TAGAAATACTACATTACAAAAATGTCACTTTTTTTTCCAAATTTGTTCCCAAGAAATGAAATTGAAAGCA
ACGTTCACATACAAGAGCTGATTAAGGAAGTGAAATCGATGCCGTGCTCTTAAGTTCATCTAGAAAATAA
TCCATGGGTATAAGCACTATTTAAATAGTAAAAGAATAAGAGGAAGCAGACAGAGTGGAGCTTACAGTCC
ACTAGTTTATGAAAAGAACATGGTTCACGAGTGCTGCAATACGTCTGTCTAGGAAACCTTCTAAATCTGC
ATCTTGAAGCAGCAGCTTGGCCGAACCACTTAGCACAGCACATGAAAGAATTAACCCACTTTTTTCAGAG
AAAGCATAGGGTCTTAGCTTATATCATGTGACAAACTGCAAACTGCAGATTTTCAAACAAAGCATCCAGA
GGAAACATGTCTGATGTCTAGCTAGAGATACAGAGTTGATATTTTTAATTCAAAATGGGAAGAATAGCAG
ATTTGATACAATTTCCTAAACACATGAAACTCAAGAAAAATGAAGACTGAAGTGTGGACACTATCCCCCT
CTTTAGAAGTGGGAACAAAACACCCATGGAAGGAGTTACAGAGACAAAGTTTGGAGCTGAGATGAAAGGA
TGGACCATGTAGAGACTGCCATATCCAGGGATCCACCCCATAATCAGCATCCAAACGCTGACACCATTGC
ATACACTAGCAAGATCTTATCGAAAGGACCCAGATGTAGCTGTCTCTTGTGAGACTATGCCGGGGCCTAG
CAAACACAGAAGTGGATGCTCACAGTCAGCTAATGGATGGATCACAGGGCTCCCAATGGAGGAGCTAGAG
AAAGTACCCAAGGAGCTAAAGGGATCTGCAACCCTATAGGTAGAACAACATTATTAACTAACCAGTACCC
CGGAGCTCTTGACTCTAGCTGCATATGTATCAAAAGATGGCCTAGTCCTCCATCACTGGAAAGAGAGGCC
CATTGGACACGCAAACTTTATATGCCCCAGTACAGGGGAACACCAGGGCCAAAAAGTGGGAGTGGGTGGG
TAGGGGAGTGGGGGTGGGTGGGTATGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATAC
CTAATAAAAAATGGGAAAAAAAGATAATACAAATGTCAAAAGTTCTATAAATTAATACATAGTAAAATTA
TAAGATTAGTGGAGTTTATAAAGTAAAATAAATATACTAACATATAGCTCCTTATTATAGGAAGTGATTA
TAAAAAATTAAGAACTTTTTCATATCTTTCTATTTCTAATTATAAAAGAAATGGGGCAGATAGTTTCAAA
GTATGAAAAGGCAAACATTAAATGAATATACTGTGGAGCTCTGTCTCTGTGGTATTCAGGAGAGATGTCA
TCTTTATACTTTGCAAAGTTAACAAAAGCTAGGGCTTTGTGAGGCAATGGAATGGGCTCAGTGAGTAGAG
TAACTTGTGACCTGCCAAGAGAACTCCCGTGGAGAAATGGTAGAGATGATTTCTGAAAATTGTTCTCTAT
TCTCCACACTTACGCATCACTCGCATCCCAACTCTAATAATAAATTAAAAATGCAAATTTAAAAATTACT
AGTCTGCATTCTCCTCCAGTTTTCTATACAGGCCGTGTGGGGATGTGACTGAAGATGCAGGCAAGGTGAG
ATGATGATGATGATTCTCATTTCCAAACCTTGCACAGGAAAGGTAAAGGCAGGAAGATCAGAGTCCAGGG
CCAGCCTCTCTAAAGAGGTAGCATATTAGAGCTATCCGGAGCATTTCAGCTCTGCACGGAAAGAGATGCA
CTGAAACATGAAACACACTGAAATGATACTCATAAAAATCACCCTCCAAATCCTGCCTATCAACTGCATT
CTACTCTATCCCGGGTCCTTAGGCAGTGCTTCCCACCAGTTCTTGCTCATCCTGAAAAAAAATCTCCCTG
CAGAATACAAGCATGTTTAGGACATAGACTTTCAATCCATCATTTCTTCTATAAATATTAAATAACAAGT
AGCATTTTGTGTGTCCTTGAATATCTATTAATGAGCAGAAAGGTCATTTATATTTCTTTTTATGCAAAAT
GTCTATTCAAGTTCTTTTCCCACACTTGCACCCATCCTTCTCTATTCATATCCACAGTTTCCATCAGAGA
AAAAAGAATTCCTACCTGTCCCCTCCAACCCCAGTTCACCAGTTTCATTTTCAATTTCTCCAGATTGTTG
TTATTTTCACCCAAGGGGAGTTTTATACAATGAGTCAGGTTTATGTTTTTGAAGATGCCTGTTTTAGACG
TTGAAACTGAGTAAGAGTTGGAATTTTCCGATGAGATGCTTTGGGGAATTCCTAAGTAACAAGTCACCAA
CATGGAAGAGTTTTACTTCAGTCGGCTTGTTTTAGAGTGCTAGCTCACCCTCTTGTCGGCGTTGATAATT
GAGCTGGTCCAACATCACCAGGACAGTTGAGCTTTGAGCTATGGACCTTTATCAGATGCCTCTAGCATTT
TGTCATTAGTGACGCCATACTGTCTGGGCTACAGCTGTGTATATGTGCACACCAATGCAATGTTTATCTG
TCTGTCTCTTACTCTGTCGAGTCAGTTATTGTGTATGAATTTGGGCATATACGTACCTGCTGTCTGTATG
TGTGCCTATGAGTGTGTATATTTGGCATGCAGCTCCACATACAAGTGTGTGAGTGTGTACATGGAGGTCA
GAGGTCAACATCTAGTGTTTCCTTTGATGGTTCTCCACCTTAAGTTTTGAGGAAGCATCTCTCTGAAACT
GCAGCTCATTGTTTCATCAAGGTTGAGTAGTTAATGAGCTCTGAGGGTTCGCCTAGCTTTGACCTCAGCC
CAGTGCTGGAGTTACCAATGTGAACTGCTGAATCCAGCTTTAAATAGGTGCTAGCTATCCAAAACGGATC
CTCACATTTGTGGACCAAGATCCTGACCTACTGAGCCTTATTATTATTTTTTTAAACATGTGTAAAGACT
ATTGAATATTTTTTAACTAAATTTATCCATCTGTGAACATTTGCCTATTATTTTTCTTTGGCTTCTAGGA
TATGATGAATTACTTTTAGACAATAAGCTTTAATTTCTAAGATAAATCTTAATTGAACATAATATATTAT
TCTTTCAGTGAACATCTAGGTTCTTTTTAATATACTAATGTGTTTCAAAATTATGTTCATAAAAGAATAA
GCTAGGGTATCCACCACAATATTAATGTGAACCAACATTAACTCAAATAGCCTTATTTGGAAGGTGGTAT
AGCATTTCATTGGCTTGAGAAATCCAGTTTATCATGGAAGGCAACACAATATAAAGTATGTGTTTTCAAA
CTCACAGCTTTAAGGTAGAAATCAGAAGCAGTCAAATGCACTATCACCACGGACACTTCCTAAACATTTC
TTTTAAAATCCCATGTAACAGAAAGTAGAAAATTTCAGCACCTGCGGTATCCCTCTAGGACTAATTCCTA
ACATTGGCCCAGCTGATTCGTGTGGTACATGGCATTCAACAGGGGCATAGCTCATCAATAATGGCACAGC
TCACGCCATGCCCAAGCCCTCCTAGAATCTTACCAAAATCAAGCATATGTTGTATCATCAAGAAAGCCAT
TACAAAATTAAATTGACAGAAATCCTAGCTGTGAAAAAAGCAAGGAAAAATGATATAAGAACTTTTCAAT
TGCTCGAAAATGAGGCAACACAGGTTTAACTAGTCTGTAGAACAGCGTAGAAGTCCCAAGAGACATCAGA
AGTGCAATAAAGATATATCATGCTATCTTTAAGCATGGGAATATTTACATAAGTGAACTTCCAGGAAAGC
TGGAGACTTTAAACTGAGAAAATAAGAGAAAACGGGAAAGTCAGTTCGGGGAAGGGCTGCAGAGTAAACA
CTCCCAGTGCAGAGAGGCAAGCAAGTTGCACTGTAAGGAGTTACTCATAGACAAAGAAAGGGGAAGGAAG
TTCTGCTAACTTCTGCCTCTGACTCATCACTGAGCAGCAGCACTTTAAGTTTCCCTGGGAAAAAGGAAAC
AATTCAGACAGGTGCCTGCATCTGCCTGTTTGTATGGATGTGGTGACGTTCACTGGCACTTGGGTACCAG
TTCTGTCAACTCCAGCTTCATTGTGAGACGGTGCCACCAAGAAACATGCCATATCTTTCCCATGAACAGC
CTTCTTTGTTTCTTCCAACAAGTATTGCTGTTTAGATTAATTTCTCAGTCAGAGGGGCATGTTGCCTCTC
AGAATATTCAGTTGATGGTGGTTCACTGTTACCAGTGGGTTTCCTCCATGCCCCTCTACTCCCTCTATCT
CAGGGGAGGTTCCCCCACAAAGATTTTTGCTTGGGCCACAAAGGCTAAATTGTTTGGATTTCATAGTATC
TTTTATATTCCTCGAGCTCTGCTATAGAATTATTTATGAGTACCCCCCCCCACCGCCAAGTCCCTGCCAC
CAGGTCCAACCTCTTTATTCTCTGTATGGTTACCTGAGAAAACAGCTTATTTTAATATAAATTTAAGTAG
TAGATTAATCAAAACAACTGTATTTCCTTACCTTTGCAAGTTTCCTAGTAACCATACAGAGAGACTAAGA
TTTATTAATATTGCCTTGAGCCAGATACTTAGCAATCATTGCTCCACTCTTAACCTCCAAGCTCATCAGG
TTTCCTCTCAGACATCAATCCCAGTTTATTTCCAGCCCTTTGTCCTTTTTGACTCAGCTCTCTTCTGATG
TTTTTAATCAACCTCTCCCCAAGGTTCCCTCTGGTTCTTTCTCCACCCTTTCTAGAATCAGAAATCCCAC
CTTGTTCTCTCTAAAGCTCTGCATTGGCTCGTTGGCTTTTATTGGCAAGACAGAAAATGAATGAGAAGCA
ATGTTTAAACAAACTTGAGACAGGAGTTTCTAAGAATAAACATAGCAAAGTCATATCTAGATTGAAACAA
GATATGAGAGCAGAGAAATCAATATTTGAATAAGACAGGGATAACCTTTATGCAGTGCACAAAAAAGTTA
TGCCTACAGCTTCCAGAAAGATATTTATATTTGGCATCAATTGTTGCCATCGTCGGTATGGAAACAGAAG
AGTTCCTGGAATGTCAGCATTGGTGAAGTAGCAATGTTAGGAAGATTCACAGAGATGATCAAACCAGTGT
TCACATATTTTCTATAATATATGCATAAGTTCTGATGTCTATGATGGCTACTCTGGCTTGGCTGAATTGA
GAAGATGCCCAGAAAACTGGCAATCCATACTTCAAGGGGTATCTATGGAGGGGAATTTGGGAAAAACTGC
TTCCTGGGTGAATGAGCAGGGCAGACAGGGATCCTGAGAGGTGGGAGTGGCCATGAGCAAAATGGACCAC
AAAAGTAGACGTGGGAAGCCAGCTTTCACTCACACTCTCAGTCCCTGGCCAAGTTCACTTATTTGTTGCT
GTTGCCTGTGGACATAAGACTCCAGCCTCTTTTAATTATTGATCTTAGAATTGCACCAGAGGCTCTGTAG
GGACTGCCAGGCCTCTGGTCTTAGACCAGGGCTGTCCCTTGGCTCTTCTATCATTCTGAGATTTCTAGCT
TTTCCAGTTAATGCAGATTTAGCTCTCCACCCTGCAAGCAGTCACTGCAGGATTTCTCAGCCTCTGTGAT
GTTCTGAGTCAGCTCAATAGGTCCTCATTTGTAATAGCATGTACTTTTGGTTCTGTTTCTCTGGAGAAGC
CTGAGTAATATGCCCAAACTCCAAAAATTTTTTCTAACAAAACTGTTAGAAATATGCCAGAGTCATACAC
AAAAGAAATGTATTCTATTGAGTATTTCCAATATCTACCCAAATTTAAAAAAAAATCAATGATGGCATTT
AATATATTTTAAACTGCATTACTATGTGCAAGGTGTTAATATTTACTTCTGTAATTCCATACAGAAAAAT
GCCATTATATGGTGTTAAGTACACAAAGCTACAAAATAAAATTTTCGTTCTTTTTTAATAGAGAGTATAG
AAAACTAAGAATAGAGTGTTGAATTAGCTGTTAGTTACATAACTGGCAATATGTCTTCCACTTCACACTT
CTCTCTTGGCTTCACATATACTATAGTGAACACACATTATTTTGATAATCAGAAGGATAATAAGAAATAT
AATTGAAAACTTTAGCCAAATAGAGTTTAACATGGATATCAGGACAACCAGAGCCCATAGGCTGTGGTAC
TCGCTGAAATACAAAAGGAGAAGACTTAATATGAAAACACATACAGGAGAGACCTCTGAAGTAGAAATTT
CTGGTTCCTTCGGAAATTCAGTTATACCATATGATGTTGGCCTGTGGCAGACAGGTGAAAGGGTGTTTTG
ACGAAGCAGACAAGTGAGGGGATGTTTTGCTGAAAGAGACACATGAGAAAGGGCACATGATGTTTAGGAA
GAATATAAATATGACTCCACAGCCAGTGGGAGGAGGCTCTGGCATTGGTTCAATTTGCTCTGTCTTGCTG
GTCTTCACTGATGGTGCTCTTGTATTGGTTGGCCTTGCATCATGTTGCTGATCTTTGCTTGTGGTGACTT
CATAGAGAGTAACTCACCAAAGAACTTCTCATGATATTCTGCCAGCTTCTTGTTGCTTTAATGGACCTGG
GGCATTCGCCAGAGCCTTGAGATTTCTTCTGGATTGAGCTGCTGTTTCTGATTCACGTTTGTGTTTGCTA
TTAGACTGAACTGCTGAACTGCTGATACCCTGACAATAAAGACTGGAATTGTCCAAAGAACTATTTCTAA
ACAGGTCTACAACCTCTGTTTCCTATTAACCTTCCTTTCCCACTACCTCTGGTGGGTGGTGAGTTAGAAC
GGAGGTTGAAGCACTTAAGAACCCTTATTAGAGTAGGTTTTGAAAATTCTAAGCCTACAGAACCTGTATT
TACATACAACATAAACAATTTTTTTTCATTGTACAAATGTGTATTTTTACGATGTAAGTATTAAAGGGCA
TGAAGAATGCAACAGGTGAGATGGGCCAACAAGACTGTCACTGCTGCCCAGGGTTCTTGACTGTAGGGCC
TATGAACGTTCTCAATTGAGTGTCCAAAAGGAAAGCTGCTTTGATGGCAAACAATCCTATTCAAAGTGTG
CTTCCTTGGGTCCTTATGGTAGTAAAGAGCAAGGGTCATGAAGTGACAAATTTTACTTAATTGCCTGTGT
GTTAGATGCAAATTAAAAGAGATTCCTTAAGTTTCTAATTCAACTCAGCCTAAATTTCCAGCCTTCCCTC
ACATCCATTGGAAAAGCTATCACTGGTCATCCTCCTATAGCCAGTTGAATGACCTAGTTTCTGGCTTTTG
AACAAAGGAAGCCAGAACGAAATTATTTAGGGAATCCAGAGAGTTGAAAAAATTCATAAAGCATGTGACA
GTTTTCTTATGTCTTCAGGAGCCTTATTTGTTACTGGTTACTGCTGGGTAGGGCATTTCCTGCTTAAGTT
TCTTACATCCTTCAAGTGCAGTTTGAATCAAAGAAAACTCTGGCACAATAGTTTATTAGTCCTATAGTTT
ATCACGTGGAAATTTTAAAAATATGATGGTTATTTATTTTTTATCACTATCTCACAGTGGCAGAGTAAAT
TGGAGCTACAGTGTTTTTGCTAAAATTGTCCCAACTTCTATAACTTCAACATCCAGGATACTGCCTGTCT
CTTCCTTGAGCCCCATGGCAACAATTCCTAAATGTGTGTGTCTAAAACAATAAACACGAATTGATTTTTG
AGTTCAGGGAATTTGCCACAAAACTAGCTTCCCTTCGAGAAAGCCTGGGAGATCACTGAGTAAATGTCTG
CTTAGTTGATCAGTACCCAGGACATGTTCTTTGCACTTTTTCTTTCAATTTTTATTATCTTTATAATTTT
CCAACAACAAAAAAATGACTGAAAAAGACAGGAATTAACTTTCTGTTTTGGATTCTTGTAAAGTTATTCT
TCTAATAAGCCTGGTCACTTCTATCACCTCCCTTACCTTGTCTACTGTCAATCTTGGTCTGACCACACAC
GACAGAATTTTGTCTAGAGGTCACACAGAAATTACAACAGAGAATAACAGTGAAAGATATGCATTTACCA
ATAAGATGCCAGAGCTTAGTTTTCTGGGTGTTTGTTTGCTTGGTTGATTGGTTTGGTTTTGCTTGGCTTT
GCTTTGCTTTGCTTCTTTTTATAGCTCCCTACACTCAATGTATCCAGACAGTGTTCTCATATAGTTATCA
AATGTTGAAATTATTGATCAAACAATCAGACCTGTGCTATTTTCTAATCTGTTTGCATTAAAAAAAATTC
ACTGAAGAAAGGGATCTTAGAATTACTTTTGAGTGAAGCATGAAGTATCCCATTCTTTCATATTACCCAG
GAACAGAGCAGAAAGTGAGGGACAGAATTTATTCAACATAGGTGCCAGAACCATCTGGTTTGGAAATGAA
ACTCAGCTCTGGACACTCATATTCATCAATGACTAAGGTTCCTATCAACCCACGTGATAGAGGATAACTA
GTCTGGACAGAGAACTTCTAAATAAGATGACCTAAAATATTCTGATCTGACTCCAGGTAAAAGGAAACAA
CCACCTATGTTATGTGCCATACTCTCTGACCTCTCAAATGCTTGTAAAGGCTGGCATTTCCTTCTCCAAT
AACATGCTCGTTTTCTTAAACCTTGAGTGTAAACAACAGCACCTTGCTTTTCATGGCCTATTTATTGTTT
GTCACATTATATTGCATTATTATTACATTGTTGCATGATACAATTCTTAAAGTTTGCTTACTTGGCCTCC
TGTAGGCTGGTTATCTGCTGACATGGGTTTCTGTGTTCTTAAGGACCTCACCAGCAAGGACCATGAAGAG
GCAGTGCTTTGAAGAGCCAGAGGGCCATGTGTCAAGGTGGATTCTGTAGGAGTAAAAGGCAAGGCTTAAA
GAGATAAAATTCATACTGACTCTTTTCCTCAAAGTTATGGTTAAATATGGACCTGTCATTCTGATAAAGA
CTTGTGTGGAGAAAACTTGAAAAACTAGATCTGAATTGTTTTTTTTTTTTTAAAACATTCCTAAAGTCGT
CAGTTCCCCACAATATAATTGACACCCATGTTGACTGTAACTCTCATAATAAATCAAAGCAAATAGGACA
TCAAAGATAACTGTGGGCTTCATTACAATTACAAAATCTAAGCAAGATAATGGAAGTTACTTGTAGACAT
CTGTTCTAAGATTTTCCACTCCTGTACTTAGAAATGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTGTGTGTGTTTTATCATTTACAAAGTATATCTGCTTTTTAAGGGGTCTAGTTAC
ACTGTTAGAGAAGCTTAAAATAGCCTACCTCCTATTTTGCTTTCCAGAAGTACACACTTTATGCTTTTCA
AGAGTAAAATAATATATTCTATCTTTTAAAATAATTAAGAAACATGCACATTACAGAATGATTAATGATA
TGAAGGATATCATATTTATAGCTTTAATATTATGTTAGATAAGTTCTTCTGATCGTTATTGGGATATTAG
GAGTTTGAATAAAAAAGAAGCAGCTGCATTGTGTTGCCTAAACTTCCCAAGTGAGTCCCAAGTGAGGGTT
TACATATTAGCTCTAAGACTAACTCACAGAGAGGAGAACTCTAACTTAGCATTTGATATCAGCAGCCCAC
CCCAAGACTTCATCTCTAGTCCACATAAGGAAGAGTGAATGCCAGGGAGGAGAGGAAAAGGCAAAAACTG
GAGGTACAATTCAACAACATCCCTCCTAGAGGATTAGCGCTCAACAGGATGCTAGCTTCTCCTTGTCCTT
CTCTTTCCCATCCTGTCATCCTGTCATCTTCTTCTTTCCTTTCTCCTCTACGATGTGTAAGAACATGGCA
AAATGATAGCCATCTCTGGAGCAGGAAAAATCATCTCACCAGAACACAAGTCAGCTGGATTCTTATTCCT
AAAATCCACTGCCTTCAGAATTGTAAAAAACAAACTCTCCCTGTTTAAACCACTCAGTCTCTAGAACTCT
GTTACGATACCCACAGCTGACTCATACAAATTATAACACATCCCCACCAGCCAGGAAACACCAGCCAGGA
AAATTGATTTTCCCTTAAATATGCCCGTAGAAAGGAATAAAATAACAGAAAAAAATTATTGAAGGCTTTG
CTTCAATAAAAATAAATAAAAATTTCCACTCTGGAAATCAGTCTGGCGGTTCCTCAGAAAATTGGACATG
GTACTACCAGAGGATCCCGCAATACCTCTCCTGGGCATATATCCAGAAGATGTTCCAACTAGTAAGAAAG
AAACATCCTCCACTATGTTCATAGCAGCCTTATTTATAATAGCCAGAAGCTGGAAAGAACCCAGATGCCC
CTCAACAGAGGAATGGATACAGAAAATGTGGTACATTTACACAATGGAATACTACTCAGCTATTAAAAAA
TGAATTTATGAAATTCCTAGGCAAATGGATGGACCTGGAGGGTATCATCCTGAGTGAAGTAACCCAATCA
CAAAGGAACTCGCACAATATGTACTCACTGATAAGTGGATATTAGCCCAGAAACTTAGAATACCCAAGAT
ATAAGATACAACTTGCCAAACGCATGAAATTCAAGAAGAACTAAGACCAAAGTGTGGACACTCTACCCTT
TCTTAGAAATGGGAACAAAACACCCATAGAAGGAGTTACGGAGACAAAATTTGGAGCTGTGACGAAAGGA
TGGACCATCTAGTGATTGCCATATGCAGGGATCCATCCCATAATCAGCTTCCAAATGCTGACACCATTGC
ATACACTAGCAAGATTTTGCTGAAAGGACCCAGATATAGCTCTCTATTGTGAGACTATGCCGGGGCCTAG
CAAACACAGAAGTGGATGATCACAGTCAGCTATTGGATGGGTCACACGGCCCCTTATGGAGGAGCTAGAG
AAATTATCCAAGGAGCTAAAGGGAACTGCAACCCTATAGGTGGAACAACAATATGAACTAACCAGTACCC
AGGAGCTCTTGTCTTTAGCTGCATATGTATCAAAAGATGGCCTAGTCGGTCATCACTGCAAAGAGAGGCC
CATTGGACTTGCAAACTTTATATGCCCCAGTACAGAGGAATGCCAGGGCCAAAAAGGGGGAGTGGGTGGG
TAGGGGATTGGGGGGGTGGGTATGGGGGACCTTTGGGATAGCATTGAAAATGTAAACGAGGAAAATACCT
AATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAGAAATATTCTCTAATAAGAAAA
AAAAATTATTGAAGGCTTTGCTTCTCAACAAAATCCTGTGTGTTCTCACTAATATCCTAGAGTGGCTACT
ATTGCTGTGATAGATTCCATATGCAAGAGCAACTTGGATTGGAAAGGGTTTAATTCAGCCCTCAGTCTAT
AATACATTATGAAGAAAATTTAGGGCAGGAACTAAAGAGAGGCTATGAAGGAAAACTGTTTACTGCCCTG
TTCTCTTTATCAGCTGACTTTTTTATATCACTAAGACTAGCTGCCCAGGGATGGCACCACCCACAGAGGG
TCATCTTCTTGATTAATGATTGATATGGGAGGTCTCAGCCCCTGTTTATTATTTTTTTCTCAACAGGGGT
TCTCTCTTCCCAGTTGATCCTGGCTTCTGTCGAGTTGACAAAAAAAAAAAAAAGTCTAACAAGACAAAGG
AAACTAACATACTCAGATCTGATGAGCCATAAGATGAAGAAACGGAATTGAAAGTGAGAAATTCAGCCTG
CCACCCTCTATAGGCCAGTGCAGAAGCATTGTTAAAGAGACCTCACACCTATTCTGGAGGAGCAGCCCTA
ACCAAGGCCCCCCCATCCAAAAGTCTTCTTCAATCCTTAGCTGGTCCAGGAGAAATTCAGCCTCTTAGCC
CTCTACCATCTCCTCATGTCCAGCTTGAGGCAGTTAGAATCAGAAGGAAGCTAGAAAGCATGCTACATGG
ACTGTAATGTTCCTGCTTTTAATTAGGAAATATGGGCATAAGACTCTCAGTATAAGACTAGATAATTAAT
ATAGTCACAAAGTTGCCCCTATGTAAAAATTAGGGAAAGTAATAAAATTTAAAATGGGAGGGGGGAATAG
ACAACGTGCCCCTCCCAAATAAAAGGCATAGGAAACACCTTGTAGCTTTTGAAGTTTAAAATTGCAATTT
GAATGTATCTATGGAGTCAAGCATTAAGTTTGAGAAAATAACAGCAAATGTTAAACGTATTAGACAGGGG
AGGTTTAGTTGCTTTCATTAATCAAGCAACTATGAAATATATCTATGTTATCTTGTATAAAGGTATTTAG
GCTAAGTGGTGCTTGAGAAGACTATGACCATGTCACTTAGCACTCTGAAAACACCATGTCTCATTCAAAG
GAGATGAGAAATATGTTTAACTGAATTACCACCCCTCTTAATGCAGTAACCAAGTTAAGACCCTTTGCTT
TGTTTTTGTTAGGAAAAGAAAAACAAAAAACAACAACAACAAAAACTCTAGAAATGGTGAACAACCATTA
CTTTCTATGAATGTATTTTCTGTGTCTGGTTTTCCTGTTCCTAACTTAGTGATCCACACCTTAAAAACAT
GCAGAGCTGTGCTGTCAAGCTGTGCACAGTAGCCACAAACCCTACGTGACCATCTGAGGGTAAATGAAGT
AAAAGAGAACAAACCAGAAATCCACTTCCTCACAGGAGCCAACCACAACTCCAAAGCTGAAAAGCTGAGG
CACATGCAATTTTGAAACATGACTTAGCCACCAGCCAGCAATACCACAGCATGCGTGAGCTAACTCAAGA
TACTTTCACCCGTGGCATTGTGAAAGCAGTAATATCTACCTCACAGGATGATTGTGCAATTCAGCATCTC
AGGCATGTGAGTTGTGTAAAATGGGCCATGGCACACAGAGAAGACTCAAAACTATTGTTGTATTTGTATT
ATAATTTTATGTTCATATTTGATTCTTTTACTGCTACTGGTGATTGGGTGACTGAGACTTAAACTGTTTA
TCAGATCTAAGAACATGAGCCAATACATTTGTACCTGAGATTATGATAGTCTCCTTGAGATGACACACCT
CTGGACAGGGTCTTAAAATTTCGAAGAAAGTGGTCAAAGATCATGAGGAGTTGGACAAAGCCACAAAGCA
GAACAGGAGGGTGAGGTTTTCAAAGGTTGAGAGCTTGAGATAAATTTCAAATAACATAGGTACTGCAGAG
GTAGAAGAATGAGAGTGGAGAAGATGTTAGAGGGATCAGGCCACGATGTTGGAAAGTGAAGCAAGAGGCA
GGGAAGAAGCTCTGTAGTCTTTGCTCCTCAAAAAATATATTTCCTTTCCATCCATAGGCCAATCCATATC
TTGTTTATTTGTAGGTTACAGCACCAAAATAGTCTAGCTAGGCTTGACTTTAATAGTGGTCACTTTTGTG
TTCTTTCATCCCTTCTCCATGTTATCTTTATATGAAAGCATTGTGCATATATGCTACTTTTAATTGAAGA
AATAGATATGGAAAGCTGTTTCCTGAACTTTGGAGATTGGCTGTTAAAAATAAACTACCTCCCTCCAGTC
CAGAGATATGTCATAAACACAAAAGATCCCCAGGTATGCACTTAAAATAAAGGATCTGCTTTTGATTCCT
TGTGGCATTTAGATAAAAAGATCCATATAAAATGTCAGTTACAATTCATTATAAAAATCAGCAAATCATC
ACAAAACAATTTTCCAGTGCTGCCACTAGAGATAATTGTTTAACTATTAAAGTGTATGCTATGATTTATA
GCAGTCACTCTGATTCCAATCACAGCGTGATCACTCAGCACTGCCACAGAAGACACACCCCATCTGTTTG
TCTCGGGAAATGTTGCATGACGACTGTAATGACGCGTACCTGCTATCACCTGCTGATAACATCCCGTCAG
AAGGAACAACAGCCTAATGACACAGCTCAGCTCCTTCTGGCAAATGGCCAGATTCAAATATTTCTACTGC
CTCCCGCAGAAGTGAGCAGCATGATTACCACAGTCCTCGGTAATAACGCCATCAAATAAAGCAATCTAGA
ACAAAGTGGTTATTTTGTAGAATACTAGTATACTTTTGACCACAAAAATCCCTTGGGGTCTATTCTGTCT
ACAGACAGTCTCACCAGCAGTGACTGCCATTCTCCTTTGCTTTAATTATTCTTGCTGATGAGACCTCAGA
CATCACTTATTTTGAATACTTGGCCTTTTGTGCAACATAGAGAGTGGTTTACACTGTATATGTCAATCAA
CAGAGTAGTAACAGATTTCTGGAGTGATCCCAAGCATTATTTGTCTACAGCATTGCTTGAAGGGCCCTTC
CTGACAAGGGAACAAAGCTTCTAGCAGCAATCCAACAGCTCGCTGAAATGCTAAGATCTTGATTAACCAA
GTTTATTTGTCAGTTGGCATAACAAACGTCAGATGCCTGGGCAAAGAAAGTGGCTACTGCTTTCCCTCTC
CCTGGCCTAGTCAGCAGACAGTATCCTCACTCTCAGTTATCATTTTTTCTTCTATAGGGGTTATGTGCAG
TTATTTCAGGTAGAGCCAGTATATCCCTGGCCTCCAAGACAAAGGACTTAATTTAAAAATATATCTTCCT
TCCATTGACCTAATCAACTACTACTTGTTAAAATAGTTATCAGAAGGCCAACTATTACTCTGGTAAAGTA
GTATTCATCAAGCTACTACTAAGGATGGTCATTTTTACCTTGTTAATTAAAATTTTTTTCTCTGATTTAT
ATTACTTCAATCATTCTCTTTAGATTCAGTATCAAGAAAGTGGGTTTCCTAAGCACCAGCAAATGCTGTG
CCATTCCTTGTGGGATGAAGCTTGCAATACTCCAAGAATTGACTTGTTGAGGGAGGAACTGAAGGATCTG
GCAGACAGACATCAAAAACTTGCTTCTTACATCAGGACAATCTCTCCACATGGAAGGCTGCCATGCTACA
TGACTAAATTACAGAAAAGTCGATTCTTTAACACACACACACATGTTGAGAACAAAGACACCTTAAAAGG
CTTTGCTATAACTTAGGACTTAACATATCCTAAAATGTTAAGTCATATAATTCAGGTGTGGTCCCCTGTG
CAAATGGTGTTTGGAGGCAGAGTTGTGGGGAAGTACTTGCATCTTGGAAGTCCTGGCTTCAGAAATAGAT
TATTCTATTGGTATATCAATATTTGAATAGGTTGTACAGAGGAGGTAGAGCTGGGTCCTAGTTAAGGGAA
GTAAGTGACTGAGGAAACACCTTGGAAAGATAAATTGACCCCTTCCTGTTATATTATGCTATCTGCCCCC
TAGCACCTTGAATTGTACCTTTGCTCTCTGTCTTATTTCTGCCTCACTACAGGCTGAGAGACACTGCAGC
CATCCAATCATGGACTTTAAACTAAACTGTGAATCAAAGAAAATTATTCCTACTTCCCCCTCTGCATTGT
TTGTCATATTGACACCAACAATAACAAGTACAACACAGTTTTATAGAATTCATATGCCATGTAAAAGCAT
AACATAAATCATCCCCATGTAAGCACGGGCCATAGCCTTCTAGGCAAGTAGCATCCAGTATACCCCTAAC
ATTTGCTTACAAGAATTTGTTTCTAAAAAGGCAGAATCTCTGTCTGTGGATTGTTCTCTGAACAATGATA
GAGGATTCACCATCTTCTATTATATTCTCATGAAGGCTACCATGTTCACAATTAAGTTACTAAAATAATG
GTAGCTTTATCACATTGAAGCTGAAGAATTTTTTATAAAATCTGGATCCTCATCAACATTGTCAGAACTA
TGAAGGAGATGGGAAGCCAAGAAACATGAATATACATTTTAGTTCTGTCTTCAAGTAGCTTCATGGCCTT
AGACAACTTATCTTTGAAATACAGAAATTATATTGGAGGTGACCCTAATTCAGTGTTGAAGTACCATACA
TAAAGTCATAGTTGACCACCACAGCATATATTGCCAAGTCTTTTCTTTCTTCAGAATATCAGATGTAGCA
GCCCAAGAATTTCATCTAATCATCTTCTTTTTTTTTTTCAGAAAAGAAAACACACAGAAATTAAGTGCAT
TGTATAGATTTACACAGTGAGATAAAGTTTGGGTTCAACAATGTGTTTTGTCCCCCCTGCCCCGGGGCCT
TTGCTGTTCCCCAGTTGGTTCTTGATCTGTCAGTAAAGAAGCCTCGAGACAATTGCTGGGGGCTTGGGGG
TCGGGGGAGGAGGAATAGACAGGACGTCCAGGTCCCTAGAGGAAAAGAGACAGGTACAACTCATCATGCT
TTGGAGAGAGAAAAGCTAAACAGCCATGTGAGGTCTCAGGAGGAATGGCCACTCACACAGGTGGGTGGTC
AGGGATGTTTACTAGGGACTAGATGTATCTGAGCCACTAAAGTTTAGGGCAGGTGGGAGGTGCAGAGATA
AGACTATTAATAAGGGCACACTTTTCCAGGTGGAAGATAATAGTACCCAGCAATTGTGCCAAAAGGCAAG
TTGAAAATGAGCAGCGTATATGTATTTTATCCATGGATTCAAGGAAAACTGGGTGGGGTCTGGTAACACA
GTCCATTCCCAGACCTTAGGCAAGGTAGGGAAAGCTACATGCAACACCCTCTCCCCAACACACATTAGTT
ATCTACTAGAGTTTACTTAGCTACCAAGAATATGTGAGCATTATGCTAGCTGCCCACACAAACTTTAGTT
CCGAGATGAAGGCAACTTGTTCCAATTAAGTAGGATTGTGATGGTTAGTCTTGCAACTCTACATGAATTG
ACTATATATAAAGGTGTACTTATTAGTAGATTTTTGAATTGCTGTTTTACAGCTTATAATTAGCCATCCT
ACAGCATCAATACTATGATTCAAAATGCTATCACACTGCTATGAATTCAATCTACTGTTTGCAAATAGAG
AGCTTAAGGGTCAGAACCAACCAGTGTTCCAGAGTAATTCTCCTTATTCTAACCCACAATAGCTCAGTTA
TCTAACTTCCTCCTTGACACTTTTGCTAACAGCACTCTCCTTTTTGCACTATCTGGCAGACCATAAGTAT
CCTGTCTAAACATGCTCTTATCGTTGTCTTCAGCACTTCCTTTAAGGTTGAAACAATGTTCTATCCTCAT
TAGCTTCCAAGAATATTTCTTCACATTTCTGTATTAAATTCAATATTTACTATACCTTTTTAAGCATACA
GTTCTCCAATTTTGAAGGGTCTCATTTATCTTTACTCCCTTTGGAGCTTGACATTAGCTTTTTTTTTTTT
TCCCTCATTACTAAGAATGTACTGACAGGTTTTTTTGTTGTTGTTGTTGTTGTTCTGTGTTATCTATTCA
GTCTTCAAACCACAATTTGTCATGGAGAATTGTTGTGTCTCTGCTGGGAGATGTTGTATGATCTGTACTA
ATGTATTATGCTAGCTAGTGATAATTTACTGTTGAAAAATATCATAAACTTACTCTCTAGTAAACTTGCT
GAACTCTCTAGTAAGTTTTAGTAATTTCTTGTTTTACATAGCTTTCATATATAAATTATGGAAGTTTACA
TTCTCCTAGTCACATTTTTATAAATGTTGATTATTACCTGTGAGAACTACTAGCAAATAATAGTCTTCGT
ATGAAGAATGCAGAATCTAAAGTGAGACCTGAGTTCAAACCCATTCTATCACCTTCTAAATGACAGCACT
GAACAGACTATTTGAGTCATATGTGTCAGGGATAAAAACCAGACACACAGGAGAAGAAGATGTTGGATGA
ACCAGTACATTCCTAACAAGGGCAGCATGTCCTGATGCCTGTCTGTGAAGGAAAACAATGCATTTAAAGT
CAGTCATTCTACACCAAGGGTACTCAACTCTAGATTGACAGCTACTAACTGCCCCATTAGATAATACATC
TCTGGAGAGTTTCATGATTACTTATGTCTGTCTCTCACATAACTTGGCAGAGAACCATACACAGAGAGAG
TGATCAGTAAATAGGCACTATTGTTCAGCAGAACTGCCCTGATGTTAGCCTTCCAGAACTAAAATCCACA
GCTTGGAGGACCTAGTTGGTAAATAACAGTGGCTGGGGTTAAGAAAGGCCAAGGTTAGTAAGATAAGTAT
ATTCATGCCAAGTCAAACAAGACCTCAGCAAAATTATGTCTTATGTTTCTTCACAAGCAACCACTAGGGC
ATTGCTTTACAAGAGATAACATCTAAAGCTATAAACTATAGATTTGTAAATCATCATTGGACTCACACTG
TGACATTATAATTCTAAGATGATATATTTAATCTTTAATATTCTTGTGAGAAGAGGCAGTAGATAAATTG
CATGTCAACAGCAAGCCTAGTTTTGTAAAGTTCCCTTATCCTCTTCTGTTGAATTGTTTCTAAAATACTT
GGCAATGAGAATTAAGAAAACCAAAAGAGCCCTGTTCGAATGAATTTAGAGATTAGGGTTTGCCGTATTT
CAATTTCCTTTCATTTATGAAAGCATTTTCTTTGTAATTGCAAATCTGCCCACTCTATAATAAAATGTGG
CATAAAATATTAGACAGCCCTGGGTTGGTTTTATTATGTATTCTATATTCTAAAACATTCACATTTCTAA
TAATGAATCCATGTATTCTGCCTGCATCCTGGGTCAAAGTATGCTCCTAGGGGATTTTAGTTCACTTCTT
CATCCTAAAAATAAGGAATACACTAAGATTTCTCAAAAGCCATATGATCTCACCAGGGATAATTCATACT
GGTCAAAACTAAATACACTTCCTCAGACACTTAGTTTTCTTTACACTCAACAAGGAGTGGCTTAGAGGCA
GCACAAATAACATTTGTAAAGCTCCATAATCTTACAGGCAAGGTGGCAGGCATTCAAGGATTCACTGCTT
CCCTCATGTCAGCCCTTCTGACTCCATTGCTGCAGCTCTGAGTATTCTCCATGCCTGAGAAGCTTCATAA
ATACGTTTCCATTAAACTGAGCATTACTGAAGCTCTATTCTATATGTATAGTTCCGAATCAAACAATTCA
ACAAATAAATAATATACACTAATAAATCAATTATCCTTGAAATTGTTCTTCTTTCTTTTCCCAATTGTTT
TTATTCTTGGGATGCATGTGTCTAGTTGGTTGGCTGGTTGGTTACTTGGTTGGGTGGTTGGTTGGGTGGT
TGACTGGATGGATGGCTTTTGGACACAAGGTCTTACCAAGGTAGCCTAAGCTGACCTTGAACTCAGCATC
CTTCTTCCTCCACCTACCAGTACTGAAATTGCGTGGTGAGTTCATAAAGGTAGAATGCTGACAAATTTCT
AGGAGCAAGACAGGAGACCGGTATAAAGTTTGATTTGGCGTAGAAACATTTATACAAGAAGTGACTTTTT
AAAGACATACATGTGAAGATAGGCACTGGGTAGACAGAAAAGTGTTTCAGAAGACAGCAAGCAGTGCAAG
TTTGACATGCAAGCAAAAATATGTGACGCTTGACCTTTTTACTTGAAGATCTCAGAAAAGAGCAAGGCTT
TCCCAAAGTCTTCACTGAGCATTGCTTCTCCCAGGGTAACCAGCTCTGTGACAATCCATTGTGTATATGA
CCTGACTCCTGAATAATAGTCTCAGAATTGGCCATAACTGATAATTTTTTACTTGCTAATTTACAGGCTA
TGCTCTTAGTCATTTGATCAATAGGCTAGTACTCCAAATGACTAATATTTTCAATTGTGTAATCCACTTT
TATTACAGTCTTGAGATTCCAAAATTAGTATCTCCTTTTCTTATTTTAGGTGTAACCAAGATCTATCTAT
CCTTGGCACCTTTAATTTTCCTATCTCTCAGGATTAATTCCCAACTCTTGTCTCATGCCTCCTGGTGACT
CAAATCTTGTCAAGAGTTAGAATACAGACCATATAGTCTAGGAATTCAACATTACTGAACTGGCAACTGA
AGCAAACAATTTAATGGATAACTAAGATGTTAATAAGTAGATTCTCTTTGAAAATACCTCTTTTTTTCTT
TCTCTCACTAGTTTATATTGTTCAGTTGGTTGATTGGTTGTTTGGATGGGTGGTTGTTAGATGGTTTTCA
AAAACAAAAACTTATGCTGTAGCCCAGACTGGCCTTGACCTCAACATCTTCTTGTCTCTGCCTGTTGGGG
ACTGGAATTGCCAGGTATGGGCAACCATGCCAAGTCTTTACTATTTTTTTAGCCTGTTGGTATTCCTTAG
TAGCAATGTATCATTAATTCGTTCATTCATTCATTGAATTTCTAATAGAAACTACCATATGCCAGAACCA
ACAAATGACAGAGCTGTGTCAATGAGGAAAATAATAAAAATATATGTTCCACTATAGTTTACATTGTAAC
AGAAGAAAAGAATTGTTCACAAGTTATTAAATCAAGCATATACACATCCAGAGTTCCTTTTTATGAACAT
AAGAAAAGCTTTGAAAACTGAAACTTTCTCTGTATCAGTTAATTCAACATGAATTGAGCAGATATATGTC
TCTATAAACTGCATTTAGTTCATTTTTTAATTGGTATCTGAATGTCTTATTGCTGCCATTTGCACCATCA
GATATTGTTTAACTTTTAAAACCTGGAAGCCTGTATTTCTAATCATATTTGATCCTCTAAGTTTCAGACC
AAGTATTACAAAGTTGCATTGTATTAGGTAACAGCAGAGATGTAGAGAAGTTGTGAGGTAATGGCTGTTG
TTGGTCATGCAATTTGAAGACTGGTGATAACAGAAAACTTCAATGTAAATGGGGTACTCAAGTCAAGCCC
CAAAGGAGGAGGTAAAGGAGAATGGACATATCTCAGATAAGAGAAGTCTAGGCAAAAATAAAATAACAGA
AACTTCAAAGGTTCTTGGGCAAGAATGTAGAATGATGTTTAATAAGCAGAAACACTGTATTAGCAAATCA
GTGAACCAAGAAAAGGGAATGAAGAGATACTAAGCATACCAGTCATTATAACTGAATTTGGATTTTACTT
AGAGGAGAAACCAGAGGAAGATTCTGAAAAGAACAGTAGTGTGTCCTAAGTTTTTGTTTGTTTGTTTGAT
TGTTTGTTTGTTTGTTTGTTTTTTACAATACCACCCTTTGTAAAATATAACTTCCAGAAATAGCTAACAC
AAAGCAGTGGCTTCCAGCCTGTGACTATCTATGCCAGGCTACTTGTCAAAAAATGATGTCTATGTCCTCT
GATCTTGAGTGGAGGATGGCCTTATGAGTTCTTTTTCCCAGAAGAAGGAAACAAGTATGGCAGTCAGAAC
TGAATGTTTTGTCCTTAAGTGTTGGCAGGTTCTACTTCCTGTCTTACAGGAGTTCAAGATTATATAAAGT
GGACATGGGCTCCAACCCTTAACCCAGAAGATTTCTGCAATTGATTCCCACTAACAAAAAGACCATTAGC
AGGTATATTTACCACACATAACAGGAGGCATCATGCCAAGCAGTAGATGGCCAACACAAAATGAACTCAG
TGGAATTTTTGTAGACTCATATTACATTCTTTGGGCATTTTTTCATCTTAGTTGGCTTTTGTTTGTATAC
TATGGTTTCTGATGTTGTGTTTTTATGGGTCCTGTGTGTGTCTGTGTTTCTTGTGGTTTTATTTTTAAGA
TTTATGGTGTTTTTGTTGGTTTGCCTGTCTGTTTGCTTTGTACGGCAAGAAATAATAGGCTTGGTGTTGG
TTGGGAATGGAGGTGGGGCGGATCTGGGAGGATTCAGGGGAGGGGAAACCAGGATATATCCTCAAACCCC
TGGGTGTAAGGAAGTTACACCCAACCAGAGGGAAAGTCTTGTGAAAGACAACTAAGTCACACAAGCAACA
GGATAAGAAACGGATATAAAGTGCCATAATCCTGTGTCCATCATTGTCTTGGGCCATAATGAACACACCC
CTGCTCAAAGGGTGCTGGCCTTGATAGATAGTACTGAAGGAACAAAATGGACTTTTGAAAGTGACAATTG
TCTGCATTTAGGTGCTAAGATTAAGTATAAGACCAGAAAAAGAAGAGAATTTCAAGCTGACTGCAATGGA
CCATACTTGTAAATACAGCACAGGAGAGGCAGAAGAGGTGAATGACAAATCAAGACCACACTATATAGCA
AGTTCCAAGACAATGTGGCACTTTATCCCAAAAACAAAATACTTGACTACATAGAAATTAAACTTGTCCT
TGCTTGTCTAGGCATCAACAAATTTTCAAAGGAACACATAGTCTTGGGGCAAAGATCAAATCCAGGGTTC
ATTAGAACAACCTACTCTCAGGTTATAGATTATATGACTTGTGTATCTGAATCTCTCATTAAGACCTGAG
AAAGTTTTAAGGTCATGTCTTGCAGATCTGACCAAGCAGTCTAAAGGTTACATAGTACCATATTGGTTCT
TCTATAGTGTTCAGATTCACAGACAAAGAGGAACCAGTCTTGGGGGTGATGTGAATATTGTCTCTTAATG
GAATGAATCTCAATAAAACTCAAAGGAAACCTCAACATTTAAAAGATATTTAAACATAAAAGAAAAACAA
ATAAGCAAATAAAAAGAATAACAGCTATATAAAAGAACGTCTCTAACTCTAACAGCAGGAAGTATACAAC
TTAATTGAAAATGTGTTGCTATGGAAAAAAAGAGGAAGGGTCCAAGAAGGAGCCAAATATCCAAAAGTGA
AGATAAAGGACAAGAAACCTATCCTCTGGTGTTCACCACTCAAGTCAGTAGTATTAACCTGGGAGAGCAG
AACCTAAAAATGAGGCAGAAGAAAAGTCCTGTACAATGGCCAACTTTATTCAGAATATCAGAACAAATTT
ATGTTCTAAGAGTTTAAAAAGAGTCACCTGAGTAAAGTGTCCAATTACAAGAACAAGCCACAGGTGGTAA
AAATATCTTTTTCCATAGAGGCATATGAATGACAACAGCTGAAGTAAACTGACTCCTATCTGCTGCACCT
TAGAGGAGAATGAAGAAACATTCTGAGAATTCTGTGTTGTGAAGAAACTGAGGTCACAGGACACATGTCT
TGTTTCCTTGGAGTTTTCTCATATGTGCTCAGAATTCCCCTCACGGAAGTCCATTCTTGGACTGTGTTCT
GATTCTCTGGGAATAAGCCTGAACTTTTATAGGCCTACTTTCTGCTTTGAGGGTAAAGAGAACTAAGAAA
ACAGGCCTGACTGAGTTTTGAACTGCTAGGAACCAGCGGCTATCACCGCCCTTTTTTCTCAGTTTTCAAT
GGCACTGTCTATTGTAATTCTCCTGTTCATATCATCTCACTCTACACTGCAGGCAAGAGAAAGTCGCTAT
ATTTTTAGGCCAGAGAAACAAACAGCACCAGAAAAAGTGTATCCATAATTTAAAGATGTAATTTAAAATC
TTCAAGTCTGAGCTACAGACAGCAATATAATAAGCCATGGGGTGAGAAGTGGCAAAGTGTCAAAGTCCAC
CATTGCTATCATTGAGATATTGAACATTATGGCTAAGTCAGTGTCGGCATGAGAAAAACAAATTAAATAT
AACTCACACTGACTTACACATTGGTTATCTATACAACTTAAAAAATGGAATTGTCACCAAGTAACATAGG
AAGGACTATGGGGACAGGTGGATTATGGAGTAAAATTGAGAATCCAACATGTTAAACATGTACTCAGGAA
TGATGTGTAGGCTTCGAATAGGAGTCTGGAGTTCACATGGAAGATCATGACTAGACACACAAATTTATGA
ATCACCAGCATATGAAAGCAGTAAAACCAGTGAGCCAGGGTGAGATCACCAGGAGAGGATCCCCAGGTTA
GACACCAGCTCTCAACTACTGGTTGAGAAATAAACAACTAGTAAAAGAATGAAATGGTAGGTGGTGAGGT
AGTAGAAAGACACAGATGGGGATAGAGTAGGAAAGGGCCCATGGGAAGAGAAGCAAGAACTACCCCATGG
AGTTAACCACAACTCCATTCTTGGACTCTTTAACAAGAAGTATATTTTTAGGGAAGAAGTGGGCATATGT
TGGTTGCAGTTTCCTCCAAGACTCAAACCTTTTATCCTCTAGAGAATCTCCTTAAGTCACAAATTAAACA
ACTCAAAATTGATTCAGGAAGTCCCTGAAACTCAGAAGACTCACTAAGAATCAAGACTGCTGAGCTTTAG
TCTTTAGCCAAGTTGCAAAGACAAAGGACTCTGAGATTAGACCAGCTGCCTAGAAGGATCAGAAATCAGA
CATGCTGCCCACAAGAGTCTCAGATCAACCTAATCACCTGGAAAGGGGGCACACTTTAACCTTTTGAGCT
GCGGGCAGGCTGTGCAGTGTTCTCTAGGTTTCCAGGTTTTATGAGCTGTCACCTATGCTGAAGTGGGCTT
TGATGATGCAGCTGCTTTTGAGTTATTTCTGCTCTTATAAGTAATTCTCACTCATAGTTCTGTAAGCAAC
TCAATTTTAAAAGTTGGACTTTGGTTGTTATGGTGGTTTGTATATTCTTGGCCCAGGAAGTGGCACTATT
AGGATGTGTGGCCTTGTTAAAGTAAATGTGGCCCTGTTGTAGTAAGTGTGTCACTGTGGGGGTGGGCTTT
GAGATTATCCTCCTAGGCCAGTCTCCTGTTTGCCTTCAGAAAAAGATGTAGAATTCTCAGCTCCTCTATT
GCCATGCCTGCCTAGCTGTTGCCATGCTCTTGCATTGAGGATAATGGACTAAACCCCTGAACCTGTAAAT
CAGCTCCAATTAAGTATTGTCCCTTATAAGAGTTGCCTTGGTCATGGTGTCTTTTCACATCAATGAAACC
CTAACTTAGACAGAGTTATCCCTAGTTTGGTAGATTGTGGGTTTCATATGCAGGGTGAATAATGGTGTGT
GTGTGTGTGCGTGTGGTCTTGTATCTCTTCAAGAAAAGCCTTCTCACACAGCAGCATAAAATTCTGGTTT
GTCTCAAACAAGTCAATGGCCTTTCTCTATACAAAGAATAAACAGGCTGAGAAAGAAATTAGGGAAACAA
CACCCTTCTCAATAGTCACAAATAATATAAAATATCTTGGCGTGACTCTAACTAAGGAAGTGAAAGATCT
GTATGATAAAAACTTCAAATCTCTGAAGAAAGAAATTAAGGAAGATCTCAGAAGATGGAAAGATCTCCCA
TGCTCATGGATTGGCAGGATCAACATTGTAAAAATGGCTATCTTGCCAAAAGCAATCTACAGATTCAATG
CAATCCCCATCAAAATTCCAACTCAATTCTTCAATGAATTAGAAAGAGCAATTTGCAAATTCATCTGGAA
TAACAAAAAACCTAGGATAGCAAAAACTCTTCTCAAGGATAAAAGAACCTCTGGTGGAATCACCATGCCT
GACCTAAAGCTTTACTAGAGAGTAATTGTGATAAAAACTGCATGGTACTGGTATAGAGACAGACAAGTAG
ACTAATGGAATAGAATTGAAGACCCAGAAATGAACCCACACACCTATGGTGACTTGATCTTCGACAAGGG
AGCTAAAACCATCCAGTGGAAGAAAGACAGCATTTTCAACAATTGGTGCTGGCACAACTGGTTGTTATCA
TGTAGAAGAATGCGAATCGATCCATACCTAACTCCTTGTACTAAGGTCAAATCTAAGTGGATCAAGTAAC
TTCACATAAAACCAGAGACACTGAAACTTATAGAGGAGAAAGTGGGGAAAAGCCTTGAAGATATGGGCAC
AGGGGAAAAATTCCTGAACAGAACAGCAATGGCTTGTGCTGTAAGATCGAGAATTGACAAATGGGACCTC
ATGAAACTCCAAAGCTTCTGCAAGGCAAAAGACACAGTCAATAAGACAAAAAGACCACCAACAGATTGGG
AAAGGATCTTTACCTATCCTAAATCAGATAGGGGACTAATATCCAACATATATAAAGAACTCAAGAAGGT
GGACTTTAGAAAATCAAATAACCCCATTAAAAAATGGGGCTCAGAACTGAACAAAGAATTCTCACCTGAG
GAATACCGAATGGCAGAGAAGCACCTGAAAAAATGTTCAACATCCTTAATCATCAGGGAAATGCAAATCA
AAACAACCCTGAGATTCCACCTCACACCAGTCAGAATGGCTAAGATCAAAAATTCAGGTGACAGCAGGTG
CTGGCGTGGATGTGGAGAAAGAGGAACACTCCTCCATTGTTGGTGGGATTGCAGGCTTGTACCACCACTC
TGGAAATCAGTCTGGCGGTTCCTCAGAAAATTGGACATAGTACTACCGGAGGATCCCGCAATACCTCTCC
TGGGCATATATCCAGAAGATGTCCCAACTGGTAAGAAGGACACATGCTCCACTATGTTCATAGCAGCCTT
ATTTATAATAGCCAGAAGCTGGAAAGAACCCAGATGCCCCTCAACAGAGGAATGGATACAGAAAATGTGG
TACATCTACACAATGAAGTACTACTCAGCTATTAAAAAGAATGAATTTATGAAATTCCTAGCCAAATGGA
TGGACCTGGAGGGCATCATCCTGAGTGAAGTAACACATTCACAAAGAAACTCACACAATATGTACTCACT
GATAAGTGGATATTAGCCCCAAACCTAGGATACCCAAGATATAAGATATAATTTGCTAAACACATGAAAC
TCAAGAAGAATGAAGACTGAAGTGTGGACACTATGCCCCTCCTTAGATTTGGGAACAAAACACCCATGGA
AGGAGTTACAGAGACAAAGTTTGGAGCTGAGATGAAAGGATGGACCATGTAGAGACTGCCATATCCAGGG
ATCCACCCCATAATCAGCATCCAAACGCTGACACCATTGCATACACTAGCAAGATTTTATTGAAAGGACC
CAGATGTAGCTGTCTCTTGTGAGACTATGCCGGGGCCTAGCAAACACAGAAGTGGATGCTCACAGTCAGC
TAATGGATGGATCATAGGGCTCCCAATAGAGGAGCTAGAGAAAGTAGCCAAGGAGCTAAAGGGATCTGCA
ACCCTATAGGTGGAACAACATTATGAACTAACCAGTACCCCGGAGCTCTTGACTCTAGCTGCATATATAT
CAAAAGATGGCCTAGTCGGCCATCACTGGAAAGAGAGGCCCATTGGACTTGCAAACTTTATATGCCCCAG
TACAGGGGAACACCAGGGCCAAAAAGGGGGAGTGGGTGGGCAGGGGAGTGGGGGTGGGTGGATATGGGGG
ACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATACCTAATAAAAAATGGAAAAAAAAATTCTGGT
TTGTTCAAAAGAGTCTAAGAATAGGGAAATCTCAGATACATTTTTTCTATATGGTGGGATAAATGACAGA
TATAGTGAGAAGGAATAGAATTTAAAACATTAGGCTATGTATTTCTTAATAGGAGGAATTGGAGCAGAGG
CATGATGGCAGGAGCTCAATCTATAAGACATGATAAGGAAGCATTGCCCATTCAAGCCATACATACATTC
AGTTCCACTGATCACCAGGAGACCCTCCACAGCAGCCAAACTAGGTCCATTACAGCTCCCACTCTGCCCA
GTTAGAAAGAAGCCCAGGGGAGAGGTTAAACTAGATCAGGCATTTCTCTGAGCACACTATCACTTGACAA
AGGGGAGTAAGACACAATAACTAAAGGAACTAAAAACCTACACTCACTATGTGTAGAAGGGATTTGACAG
ATGAGCCTGGTTCTCCTTTCCTGATGCCTGAGGAATGACCCCCAACACACACACACACACACACACACAC
ACACACACACACACACACACACACACACACCTATAACCCTTTCCCAACATAAACCATAAAATACAATTGA
AAAAACATACATTTATGAAGAATTTCAGCAGAGAGACAGTTCCTTGATCGCCTCAAAGCTTGGTCATGAT
TTCTCAAAAGCTGTCAAGTCTTTCACAGTTCAACTGGAAAGTTCAAGAGTAAGCTGTCCATCTAGAACTG
TGACTCAATGTTATTGGCCGCCAACAACATTGCCAGGGCCTCTTTCTGTTCACAACAATGAGTTCAGCTC
CCTTCCCCCACCAGTCCTGTGGTCATTTCTTCCCTAGGCCCCACCCTTGGCCATTTTCAGTTTCTATTTC
TTCATTTGGCAAAATAATTTTTTATCATCATCCTAATGTGGTCTGTGACCTCTCAAGAAGACAGAAGAGA
TCAACATTTGGAGAAAACACCCATCTTTTACACTCAGGTACATAGATCACCATCATACTGTCTAGTTCCG
TGCCCTGTAGCGGGATAAAGCACTTAAACACACATGCGTACAACACTCGAGTCTCCAGACAGAGAATGTG
TATGATCACTGCTAATCAAATGGCTCAAGTTACCTCACTTTGCTAATTCTGACCTCAGCCACTCAACTCC
TTAATTTTCTTTTGACTTTTATCATCATGGTGTGTGTCTGCACGGTGGCGGTGACGGGCTCATGTGGCAT
GGCACATATGCAAGTGAGGTCAGAGGACAACTTTATGGAATGAGTTCTCTTTGCACCTTTGAACTCAGGT
CACCAGGCTTATGTAGCCAGCTCCTTTACTCACTGGCCAAGCCACCTCACTGGCCCAGGATAACACTTTA
GAAAGGGATAACATTGTTTAATATACAGCAAAATAATCAGTCAAATGATGGCCAGTGTCTGCTTCCTGGC
AGAGATGGTTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTCCTTTC
TCACCATACTAAACACAAAGAACATGACAACCTACAAAATGAGCATTGGTAAGAGTGAGCCTTAAGCTCA
GCAGCAGATCCTACTCCACTAAGGAGGGACGGCAGCACCACCATTACCGTTTTCCTTTCAGTACAGTAGG
AATAAACGTGGAATGGCATTAGAATTCAACAGGACTATGCCTAAGACAAGCTGGCATGGTGCCTTCCTCT
TAGTACTTTCAGGTTCACACTAGGTTCCTTCTGAACATGTTGTTTTACTTCAATATGTTAGGATTTCTAG
GCTAACATTGATTTTTCCATCCAACTCTAATCCAAAAACAATTGCAAATAGCCCTTCTATCTCCTGACAG
CTGCTAACATATTCACAAACACACATATACAAATCTATAGGATATATCAATATATGTATAAATGCATATG
TAATTTTGATATTAGTTAACTGAAACACCATAAAATAATTCTTCATCGATTGCATGCCATTTCTCCCTAA
CAACACTCACTTTCACTCAACACCCTGATAGACTTTTTCCTCCCACCAGTGCAAAGGCAAGCACAAGCCT
CACACAATGTTGAGTTGCAGCCTCAGGTATGATGTAATGGGAGATTCTTCTCCTCCTATCTCTCTTTGAG
TCCTAGCTTCCTGTGTGGATGGATGGAATGAGAAGCAGAGAGATTCAGATACAGAAGTCAATCCAGATTT
ACCCATTATGAGTCATTATTTTAAAAATCTGATACATATTAGGCAGCTATTCCCATTTAGAGATCATGAA
TTTCTCTAAGGGATCAAATGCATACAGATATTATGTGATTATAATCCTTAAATATATAATTAATAATATC
AGCTATAAAAGTAATCAGAAGCCAAAATTCTAGCAACATTGGCCCAAAATAAATTAGTAACTGTCACTGT
CAAAACTGTGGGGAAGCTGTTGAATAGGAAATTCTTTCATTTTTATCAGCATTGCAATCTTAGCTCTGTC
TGGGGTGGAAGAGATTAACCCTCCCAAGACCTGTTTCCCTGCAAATGAGTTACTCTAGCAAATGTGCCAT
ACACACGAATATTTATAAACAGATGTCTAGCATGACACTCTCTAAACACATGTGCACTTAGAACAATATT
GGGGGGTTAGAATTTAAGTTGTTGACTGTTATTTATGTAACAGGCTGTGGGATGAAGACATTATTTCCTG
GGTTGTGGTTAACCAGCATTCAGAAGAAAAGTTACATAAACAGAGACCAGATTCTAAACGTTTTAAAGCC
TTGGGTCAAATATCCATTTATAAAAGCTGATTGGTGTTACAAATAGTTCCCTCAGGAAAATCCACAGAGC
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTAGCTTCCCTGAAATCACAGAGCTGAAATCACAGAGCGGCA
GCACACACCTCAGATGAAGTGCTTCCCAAGGTCAAAATTCTGCTTCCAAATTGTTTCAATAACCTTAAGC
CATTTTGCTAAAACTCTTCAAAATTAGTTTATATATATATATATATATATATATATGTATATATATATAT
ATATATATATATAAATCTGTGTCCTTGACAAATGTTATATTATGCAATATAGCCACAGACACTTCTTAGA
ATTGAAACAAGATTTAACTAAGAAAGCACCATTGTATTTTTCCTTTAACCCACCAGCAATCTGCAGGAAA
ATTCTAAGAAGCAGCTTCCCACCGCACTGTACACTGCCTCTGCCTGCCAATCAGCAGCCAGCAGTAATAT
CTAAACTACTAGAAACCTGTCAGGGATCTGAACTAGGGAAAACCTGACCCCCTATCATGTTGCAAAACAG
ATCACAAGGCAAGAGGAGCCATTAGAGTGAGAATGGGACTGCAAACTCCCAGAAGGGAAAAGGGGAAGCA
TAAAGATCAGAGGAGAAGCAGACACATTTATAACAAGCCTGAAGCAATTTCTGACCCTTCCACATTTTAA
GTAAAACTCCGGAGTTTGTTGTTGTTGTTGTTGTTGTTTTTTTATTCTAAAAGCCTAAAATAACTATAGT
TTTGTCTCTACTCCTTTATTAATATTGATGTTCCCTCTTGAGTCGCTTCTTTCTTATCAATCCCTCTAAA
CTTCCTTTAAGAAATCCTTTAAATTTTAGTGAGCATTCAATGTTGATAAGGATCTAACTTGAGGCAGCAA
TCGTAGTAATCTGTTTATTTACCGGCTCAGACTGGAGAGTCCTGGCAGGTCAATGGGGCCTCCCTGGCGC
CACCAATGAGAGTATCAGAAATCAATTTAAAATCACCAGTGGAAAGTAGCTGCTGCCAATCACCTCATTT
CCATTTCAGCTTCTGCGGGACTCTGTCTCTGGTTCTGTCTGAAACCTGTCAGGACGCTGCCTGGTTCTGT
CTGGAACCTGGATCATTAACATTGTTTGGGCATTAACATAATATTAAATGCAGGGGGGAGGGGCTATAGA
CATTCTTTTGAGCTTCCCTTTCTTGCAACTTTGCACTCTCCAACCCACTTGAAAAAGTTAGCTGGGTTTT
CATCTAAAGCCCGCTTGAGTGAGGATTTTATTTCAGGACAATCTTCCCCGTTGCTTCCCTTCAATCTGGT
ATTTCATACAGACAACTGCTTTGATTAAGCCTTGAGCTAAGCTGTTGCTAATACAGACCCAGACCTATGG
CCATTCCTGCACATTTATTTCCCTAGAAAACGCATCTTCAACAAGGAAAAGGTGAGTAATTTTTGCAAAG
GGAGAGAGGAGAGAGAGAGAGAAAGAAAGAAAGAAAGTCCATAGCAGCCATCCATTCGCCGGGGCCAGAA
GTTTGAAAGAATGTGGCAAGCAGAGCCGAGTTAGTACCTTTCCTGACCTCTCTGAGCTGTTAGATGTGGA
ACTCAGCCTTCCAAACACAGCAGGATCCCGTCTTCGTCCAACTCTGCAACTGGGCGGGGGCAGTCCTGCC
CTGGCAAGGTCCCTGCAGCGCGTAAGGAGATGCACTGCAAGTCTTTTGGGCGCAGAGAATTGGGAGAGAC
TTGCAGGAGGACCTGCAAGCTGCCCTACTCTGCAAGGGGGAGGATGAAGAAGAGAACTCTGGGGAAGAGT
GGCTGGACAGAAGGGGAATACAGTGCCACTGAGTATTGAAGTCTTGGTCAAGA

Protein Sequence : Show Sequence

>gi|363000984|ref|NP_001241676.1| interleukin-15 precursor [Mus musculus]
MKILKPYMRNTSISCYLCFLLNSHFLTEAGIHVFILGCVSVGLPKTEANWIDVRYDLEKIESLIQSIHID
TTLYTDSDFHPSCKVTAMNCFLLELQVILHEYSNMTLNETVRNVLYLANSTLSSNKNVAESGCKECEELE
EKTFTEFLQSFIRIVQMFINTS

Molecule Role : Vaximmutor
Related Vaccine(s): MVA/IL-15/5Mtb vaccine

26. Il17f

Gene Name : Il17f
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 257630
NCBI Protein GI : 119360346
Genbank Accession : AC159614
Protein Accession : NP_665855
Taxonomy ID : 10090
Chromosome No : 1
Gene Starting Position : 20777145
Gene Ending Position : 20784269
Gene Strand (Orientation) : -
Protein Name : interleukin 17F
Protein pI : 8.94
Protein Weight : 16848.95
Protein Length : 161
Protein Note : Also known as C87042; IL-17F

DNA Sequence : Show Sequence

>gi|372099109:20777145-20784269 Mus musculus strain C57BL/6J chromosome 1, GRCm38 C57BL/6J
ATTTTTGAATTAAAAATATTTTATTATATAATTAATGGATATATTAATTTTCTACTAAATTGATATATAA
TCAATAGGTCATAAATATATGTATAGTTCCCCTACCAACATAAATATATAATAGTTTATGCCAACTTCAT
TTATATTTACCAAATTTTTCTCTAAAAACACACATACCATGTGTGCTTCTTCCTTGCCAGTCCAGCCCCT
GCTACACAGGCCTCCTCCCATGCATTCTGATTATGTTTGTTTTCTTTCCATCACAAATATGAGCATTTCA
TTGAACTCTACAAGAATGGTACTGCATCAAAGTGACAGTCTTGACAGACAAGAACTGTGGTCATCATCTA
ACACAAAGAGACTGGGCCAAGGGTGCCTTCATTTACCTTTGTTTTTAGGAGTTTAAGTGAGGCATTGGGA
ACACACCAGGACTGGTAACAATTCTAAAGCAAGCCAGAAAGTTCAAGCTATATCTACTGCAGAGCTGCAA
GAATGCCATGCACACCTTACTGAGAGTCCTAGAAAGCATAAAGACTTGGAGATCGGGCTTCACACAGAGC
TCTGGATAAAAGGAGGCTTTTTCTCAGTGGAAGTCTTCAACTGATTTTTGTATGCAGCGTTGTCAGGCCG
CTTGGTGGACAATGGGCTTGACACAGGTGCAGCCAACTTTTAGGAGCATCTTCTCCAACCTGAAGGAATT
AGAACAGCCCTGGGGCTCCCTCCGAAGGACCAGGATTTCTTGCTGAATGGCGACGGAGTTCATGGTGCTG
TCTTCCTGACCCTGGGCATTGATGCAGCCTGAGTGTCTGCACTGGGCCTCAGCGATCTCTGAGGGGAACC
GGTGGGGGTCTCGAGTGATGCTGAAGGAAAAAAATAAAAAGTAAAGCAGGATGCTTGAGGCCAGGGAGAG
GAATAGAGAAGGGTTTCCGCAGCACTAGGTGTTTCCCTTCAGGGGCAGTTCTGAGTTATGCAGGGAGCAG
CGCAGACTCTGAATTCTTGGACCAAGCTCTAGCTGATAAGTAACTTCCTAGACCTCAGATGCCTCCACTA
TAAAACGGGGTTTCTCCCATATCATTCTCTCAGAGAGTTGTTGGGGATATCACATGGTTTGAATGCATAC
AGAGTAACCCCATAAATACTTTGGTTACTCTTAAATCTACATTTTCTTTGCATTAGTGTTATTATTCATC
CTTTAAGCAGAGGTTGAACATCACTAGTAGGTCTTCCAAAGTACAATGCATAAAACTCACTGCTTGGAAA
GCAAAATGAAAGCCTTCAAGGTCTGTGAATCCAGAGCCTCACTTGGTTCCTCTCTTGAGACTCCCCTCTG
GCTGAAGACAGGCAGCCAAGTGAGTCTACCACAGTCTACAAACCTCTCAAGAAGGGAGCTATGTATCAGC
CTCAGAGCACTGCTTTGTCCCTAGAATCTGTTTTTATTCGTGTATTTGAAGAGTATTGATGGGTGGTGAG
TGTTCCCAGGACACAAGACTCTGCCCTATACTACATCTATTTCCCAAGCTGACTTTTCTTCCAAATCCTG
ACTTACCATCCCTACTCAAAGAAAATGTCTAATCTCAGTAGATCCCTAAACGGATTCATTGTTTCCACAT
ATCCTCACAGGCTGAAAAAAATATTAAGACTTCCAATCACATAGGTGACCCAAGGCAAGAAACTGAGTTA
GTGGGAAAGGTGCAACTTCTATTGGAGACGGTGAATGTGAAGCCAGGCCTGGTGGCTCATGCCTGTAATC
CCAAAACTCAGAAGACTGGGGCAGAAAGGATAACCATGTATCTGAGGCTAGCCTTGGCTACCTCATAAGT
TTCTGGGCTACAGAGTGAGGCCTCTATCTCAGAAAAAAAAAAAAAAGTGAATGTGAGTCCTGAATACTCA
TAAGAATGAGGCATTGTAATCAAAATAATGTAGCATGTTTTTCTTTCTTTTCTTTTATTATTTTGGTTTT
TCAAGATAGAATTTCACCATGTAGCTCTGGCTGTCCTGGAACTCATTACATAGACCAAGCTGGCCTTGAA
ATTAGAGATCTGCCTGAGTCTGCCTCCTAAGTGCTGAGGTTAAAGGCATCCACCATTACATTGGGTTTAG
CACATTCCTTTCCATGTAATGTGTCTATCTCTCTTCCTTGACTTACTTGTAATCCCATGGGGAACTGGAG
CGGTTCTGGAATTCACGTGGGACAGAAATGCCCTGGTTTTGGTTGAAGATTCGAATGTCAACTCTCACAG
TGTTATCCTCCAGGGGAGGACAGTTCCCAGCCTTCTGCAAGGCAGGAACCCCTGCTTTGGGGTTCTTCCG
AGCTGCTACCTCCCTCAGAATGGCAAGTCCCAACATCAACAGTAGCAAAGACTTGACCTGGAAAATAGAG
ATTACCACAGGTTAGAGCCCTGGTGGTGGGCAATGTACTTGAACTCTGTATAGGGTCACTTTACAGGATC
AAGGTTTCTTTTTTTTTTTTTTTTTCATGGTTCACTAGCAATGAGAACTGCCGTGTTTAATGTTATCAGG
ATACAAAGCAGAAAGGGTCAGGAGTAAAGCTCTCTGGAGTTTATCTCCGGCCTTATAGTCCACAAAACCT
TTCTCTGGGACATTCCACCAAACCTCACCCTACTGTCAAGGTCCCATATGTAATTCAATTAGAAGTATAT
TTATTGGGATGGAGGAATGGCTCCACAGCTAAGAGCACTGATTGCTCTTCTAGAGGTCCTGAGTTCAGTT
CCCAGCAACCACATAGTGGCTCACAACCATCTGTAATGGCATCCAATGCCCTCTTCTGGTGTGTCTAAAG
GCAGAAACAGTGAACTCTTATACATACAATAAATATTTTTTTAAAAGTATATTTATCAACATTAAATAAT
AAAGATTTTAGCATGTATCACTCAAGAAGTATTTTTTCTGTTCTCTAAAGATGTCATATCTGTGTTCATA
CCAGACTCAGAGTTAACTTCCTGCGAGAGCATAGTTGGTCCATGAAAACTTTCTGAATGAATGAGAGAGT
GGGGAAGTGAATGAACAGGGCAGCCCAGTTAAATTAACCAGTATAGTTATGCCACGCTCATTGAGTAAAA
GGATTATGGGAAACTAAGCACAGCAAAATTTCATTTATCCATGCTAATTGAAAGAAGCAGGGACACTGAT
GACGCCTGGCATCACTCAGAGTGCAGAACCCATTTCTTTTAGCTCTAGAATCTATCTTTTCTGTACTAAC
ACTGAAATCAGTTTCGCTGCTGTTACTAGCGCACACAAAGTGCTCAGTGGAGCTCTAAACACAAGAAGAG
CTAAAACCTCAACAGCTCCCCTTGGGTGCAAATGTCCTTTGCCGTCCCTGCTCACACCTTCCTGGGGAAG
AACAAAGCCCATGGTAAGGCAGAAGAGCTAAGGCTGAGCTTTTCATTTCTGGGCTCCGTGTTGTTCAGGC
TCACACTGTGTAGGTAGGGAAAGTGACTGTGCATGTTGGATTTAAAGATTTGCCTGTTCTTTTCAACATC
CTGGAGGCAAGAAATATGCAGAATAAACAAAGAGGTCATGGAGTCAGCCTGCTGGACTACGATCACTGAT
AATACATTGAAAGCAAGCTGCAGGATGAAACAGATTTGTCTCCAGATAATCAGCAGCCATATGCCTTCGC
CTCTGAAGTCATTCGTTCACTGAGTAGCTAATCCTCGAGGCCACAGTGTTGTGGTTTCAGGCATTATCTC
TAGAGTTAACTGTGTGGAGTCCATAATTCATTTATAGGACTCACCGCACAATGACTTGGTTTCCTACATC
CTCATAAATCCCAAAGATCCCTGTAAGCATGCAGTAGTTCCACAAGGCAACAACGGTTGGCAGTGTATTG
ATGTTACCCTGTGCATGGGATGGATGTAAAATGCAGTCTTCACAGGTGCTTTGAAATTTAGAAAACATAG
TCACACATCTATCAACCACTTCTTTAGATTTCTTAAGTAAATTTCTCCTCCAGAATCTTCTCTTTCTTCT
TATATGTCATGTTTATTGTCTCACTTCTCATTTTGACTAGCAAGACTGAAAGTCACTATAGTTCGGGGTT
ATGTGATTAATGTAAGTTGTTGTGGTGGTTGAACTGCGAAGCAACCTGCATAGGCTTATTTATGTGGATG
CTTGGTCCCCAGTTGGTGGAATTATTTGGGAAGGAGTAGGAGGTGTGGTCTTGTTGGAGGAGGTGTGTCA
CTGAGGTGGGCTTCGAAATTTCAAGTCTTATATCATTCCCAGTTAGTTCTTTCTGCCTCATGGTTATGTC
TTAAGATGTGAACTCCCAGCAACTTCCCCAGCGCCATGCCTACCTTCCTGCCTCCAGCCATGATGATCAA
GCAGCTTAACCCTTGGCAACTGTAAGCCCCAAATTAAACACTTCCTTTTATATGTTGCCATGGTTACAGT
GTTTTATCACAGCAATCGAAAAATAACTAAGACAACGATCATGTCCCTAACAGCTCATTATGTTTCTTTA
TTAATAGCTAGACATTCTTGGAGTATTTTAGAAAATGTTTTCACACAAGATTTGAACAGCTAACTCATAG
ATGAAACTCATCCTAACTATCTAATCAGCTCTCAAAGGACTTATGTCAGAGAGGCAATAAACAACCCGGC
AGGGTTCCCCAGCCTCATCGTGACAATCTATAGGATCCACCTTCTATGGCAAGGCTTGGCCACAGCGACT
GAAGGTCATTACTATTCTTCACATTAGTCTTCACTGTGTCTAATTTCAAGAGCCACGAATTCTAAACACT
CTCTGCACTGCATCAGTAAGACAGAAGTATTGAAAGCCACAAACCACTGTTTGCTTTATGCGTTTTTGGG
GTTTTGTTTGTTTGTTTGTTTGTTTTTTGGTGTTTTGCAGCTCTGATAAGGCTGGTGCTGAGTATGGAAA
AACAATAGAAACAATCCATCTTCTCAGGGGAGTCGGGAGATTTTACAGGTTTAAATTCCACCTTTAAAGG
GGATTGATAGGTGTGATTCAAATCCTGTAGGATGGGAAACAGCTAGGAGAATATTGAAACTGAAGGTGTT
CAGTCATAGAGTGTGAATCTTCCCAGCTTAGCTTTGTACATTACTCTTGTGTGCTCGCTGGTGGCAGAAC
ATCTGCACACTTGCCCCTACACTTTCCTCCCTACACAGGAATGGGTGACAGAGACTGGTCAGCTCCAAGC
TCCATCCAAATTTCCCTCAATTGCCCACAGCATATGTCTTCTCTGCTTGCCCCTCCTCATAATCCCGTTT
CAACACTTCTTGCTCCACCATGTTTCTTGCTGACAATCCAACTGATTTTTTCTCCCCCAAAGCAATAGTA
ATGGTTTGGAGCATTAAACTATCCAAACGATTTTCTTGGCTACAATGGCATTAGGTTGAGAATCTTGCCT
CTCTCGATCATCCACAGATAAGCACACTATTGTTGGGAGTCAGAAAATCTAGATGCAGGTCTCAATATTA
TTATATTAGCAATATATTAGCATTGCAATAGAAAATGACAGCAAATGAAAACAGATGACTGAGCTACTGT
GTAATGGAGAAAATGTTCGTGATCTATTCCTGTGGGTTGACAAGGGTCTGAGAGGTAATAGTAAATCACA
GAGCAAGGCTTGGTGGCACACTCCTGAAGTCCCAACACTCCAAGAGAGCAAAGCAAAAAAGACAGTTCCA
GAGCAGTCTGAGCCCACATAGCAAACTCACGACTATCGTGAGCCACACAGAAGATCTTGTCTCAAGGGCT
GGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCCTCCAGAGGTCCTGAGTTCAATTCCCAGCAACC
ACATGGTGGCTTACAACCACGTGTAATAGTACCTGGTGCCCTCTTCAACAGTGTATTCACATACATTAAA
TAAATAAATAAACAAACAAATAAATAAATAAATCTTTTTTTAAAAATCTTGTCTAAAAAAGCAAAAACAA
TAATATAAAGAATTGGTATTTGTATACTCTTTCTACCTTTACCTCTTATAGCAATTGCAGTGTCCAAGTT
AACTAAAATCTAAGCACTTATGATCTACCTTGCAAGTCTAGATTCATATCAATATAACAATCTCTTCCCC
CATAATTATGCACAGGTAGGGGAAGTAAAACTCACAAAGGGAATGACCAACACCAAAGTGCATACTTAGC
AGGTAGGAGAGACAAATCGGTCTGACTTCAAAGCCTCTGTCTCTAAATACTGTATTTTGGTGGTTTACTG
CAATCACTATAATACTATACACTATGCAGTAATTAAAAGAATATGGCCATTGTCGGAAAGCATGAGCCAT
TTATAAAAGCCATGCTCTTTCAGGTCTCAAGGCTTAGCCAGGGAAAATCAAAGGACTATCAAAACCAATA
ACACAGTGTGTGATTTCCTAAGCTTACTCCATGTGCCTCTATATTAAACCCTTCCCAGGTATTAATTAAT
TAGTCAGTTACCAAAGTCCCGAGACACTTCTCCAGGAGCTGAGCAAAAGTTGAGCCCTGGGCTATTCTAC
TTTCTCCTCCATCCAAATGACCTTGGAGGAACCAAACCATGGGTCCCAGCTTCTTTGTGGTATATATTAT
ATATTAATATTTAATGTATATTTATACTACTATCGCATGCAATGTAACACGATATTATATATAGATTCCT
GCCTTGGAATACATATTTATATAAAACAAAGCTTGGCCTGGCTCTTATTTTAGGTCAACCTAAGTCTTTA
ATGAGTAGGTAAGAGAGACCATGATGGTCACTGGAGATAACCAGATACACACACCAGCCCACCTGAGCAA
ATCTATCAGGTAAGATCATCTGCTCTTCTCCCATTCTAACTGTTCCATTGATGTTGGTTTTTATTCTTCT
CTTCTCTTCTCTTTTTTCTCCACCAGACAAATCAAAATTAAACTTAACTTCACGTTTTAAAGAGCTAATT
CACAAAAGACTCTAAGAAGGCCTAAAGCCTTAGAAATCTCCCTTTCTTAAAGCTTAGACGCACCTATCCT
TAGCCTATGATTATTCAACACCACTCACCATGGCTGTTTCACGGGTGCACTTCATGCTGGTTTCTCCAAT
GGCTGCTTCTTCCCTCAGGAAGTACTTTCTTCTAAGTATGTCTTCCTGGGTATGT

Protein Sequence : Show Sequence

>gi|119360346|ref|NP_665855.2| interleukin-17F precursor [Mus musculus]
MKCTRETAMVKSLLLLMLGLAILREVAARKNPKAGVPALQKAGNCPPLEDNTVRVDIRIFNQNQGISVPR
EFQNRSSSPWDYNITRDPHRFPSEIAEAQCRHSGCINAQGQEDSTMNSVAIQQEILVLRREPQGCSNSFR
LEKMLLKVGCTCVKPIVHQAA

Molecule Role : Vaximmutor
Related Vaccine(s): MVA/IL-15/5Mtb vaccine

27. IL2

Gene Name : IL2
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 3558
NCBI Protein GI : 28178861
Genbank Accession : AC022489
Protein Accession : NP_000577
Taxonomy ID : 9606
Gene Starting Position : 123372624
Gene Ending Position : 123377649
Gene Strand (Orientation) : -
Protein Name : interleukin 2
Protein pI : 7.95
Protein Weight : 17130.78
Protein Length : 153
Protein Note : Also known as IL-2; TCGF; lymphokine

DNA Sequence : Show Sequence

>gi|224589816:123372624-123377649 Homo sapiens chromosome 4, GRCh37.p5 Primary Assembly
GTTTATATTTATCAAATTTATTAAATAGTTTTACTAACCAATCTACATAGATACTATATTTAACATTCAA
CATAATAATAAATATTTTGGGATAAATAAGTGAAACCATTTTAGAGCCCCTAGGGCTTACAAAAAGAATC
ATAAAAGATCCATATTTATAGTTTTAAGATTAAGAATAATAGTTACAATAGGTAGCAAACCATACATTCA
ACAATAAATATAAAATTTAAATATTTAAATAAATAGAAGGCCTGATATGTTTTAAGTGGGAAGCACTTAA
TTATCAAGTCAGTGTTGAGATGATGCTTTGACAAAAGGTAATCCATCTGTTCAGAAATTCTACAATGGTT
GCTGTCTCATCAGCATATTCACACATGAATGTTGTTTCAGATCCCTATAAAAGAAAAATGTTAATTTTTT
AAAGTACAGAGTAGTTTACCTTATATACAGTTATTCCCAATTGAAGTCTTATAGGCCTGTTGCCTTTATT
TTCAAGCTTACCAAACATATTAATTATTCACATTTTCTTGAAATGATCAAAATGAATGCCAAAATTACAT
ATTTTGTTATGATACCAAATGATAGTAACACAGAAGCTGAGATTTTCCTCATTTGTTAATTCAGTCAATG
AACACAAAAATTTTTTCCCTAAATTGGGTGCAAATAAAGATTTAAATAAAAATAGATCTAGACAAATATT
AAATGCTGGATGTGAAATAAACTTGATCGCTTTTCCTCTGAATGTACACCTATATTTGTGTAAGTTAAAT
GTATGAATTGATATGCACATTATACTTTCTTCTTTTCTAAATCTTGATTAGAGTCTCCTATTTTTCTACT
TAAGTGAATCTCCTTATATTTTCCCCAAAGCAGAACAGAACTACACTAGAGTAAGCTAGGACATGCTTCT
GTTTGGGATAAATTTGTGTGAAGAACATCTTCATAAATACTTACACCTATCCCTACCCCATCATAGTATC
AATGCAGGTGAATTTGCCAACTTTTAAAAGTTTAAACATTAGAAATTTTGTTCTGTGGACTGGCTTTTTA
GAAATTAGGCTTCTCTGCTGAAAGGAGCTATTTAAACGCTTCCTCTAGTTGATAAGCTAAGGAGGAAATA
TTTTGATATATAGTGAGATTTAAAAGAGAGCTTTTCAAATTTGAAGTGATTCCTACCCTGGGAACAAAAG
CAGGTGAGATAGAGTGTTCCCTTTAAATAAGTATTCTCTTTTAAAAAACTGTTGCAGTGTTCTGTATCCT
CTTAAGCATAATTGTTTAAATATTGTCATATCTTGGGATTTTTTTATTTTAAAAAGTGTTTGGCTTTTGA
AACACAGTTTTCAGATTTTTGCATGGTTTTGTCTTAAATTTGTATCAAGAGTTAAGAATGAGTTTTCTGA
TGTTAATTATTTTAGAGTAAAACTCTTGCCATAGATAAGGAACTGAATCTGTCAATGTCTAGATGACATT
GATCTCAATGTATTTTTTTAATTCAACTGAAACAGTCCACTAATAAGTGATTCATTTGGACACAACTGGA
GTTACTTTTAGGAATGTCTTTTACTACTATACTTATGCTGCTTATTTAGGATACCTATTAACTCATGAAT
TTTATACCTTAGGAGACGGGATATAAAAAGACAAAAGTGAATCTCTTTTACATCACCTGAGTCCCTTGCA
TATTTTATGCTTCCTGTGCATTTAAAAATACTTAAGATTCTGAAAGTGGGGATTGCCTCTAAAAAGATTG
TTGTTTGTTTAGTCCACTGGCCATTTTAATGGCTCCAGTTTTTTTTTTTAAGACAAATCTCTTATGGGTA
GTCCTTCCATCAAATGATAAGGAAACACCATTTGAAAAGCCTTTCAGGTAATCAGTATTGGCTATTCCAG
TAAATGATCTGAAGGAATTGAAAAATATATAGTAAAATCTGCCTGCTTTCTGTGAAACTCAACTCAGTAC
CTTCTCTCACTCCCAGTTTTGTGTTAATATGAAAGGTGATGGAGTTTGGGATGAGGCTTGTCCATTAGTA
GATGGTAGGTGCAAACTCAGTAGCTTATACTCCCCAGTTAATCATTTAACATTAGAAATGATTCATGTTC
ACAGTGTACTTAAAATGTAGGCTAATTACATGCATGGGTACTTTACAAATACCAAAAATGTTATGTCACT
TTAAAATGTGGTACTTTTCCCCCTACTTTTTTTTTTTTATTTCCAGGAGAGCAAATAAAGTAATGCCTTA
CCTTTAGTTCCAGAACTATTACGTTGATATTGCTGATTAAGTCCCTGGGTCTTAAGTGAAAGTTTTTGCT
TTGAGCTAAATTTAGCACTTCCTCCAGAGGTTTGAGTTCTTCTTCTAGACACTGAAGATGTTTCAGTTCT
GTGGCCTAGAATAATAATTATCATCAGCTCAGTTTACATAGAGGTCAGAATCAGAAATTAATTTCCAATT
TATTTTATTTGGAGTAGCAGTATGTAGTTTTTACCATTCTCTCTGTTCTCAATGTCACTGTAAAGATGGA
CCAGTAGAGTTTACATTATAAAACTGGAAAAACTAGGGCTCAAGTACCTATGACTAGCGTTAAGTGATAG
TTGACTCACTGGTTTTAGCTTAATTTGGCTTTGCAAAATGTTAGACTTTCTGCATCTAAATGCCTATTAC
AGATGTAGGCTGATTACTTTTCCAATCTGCATAGAAAATCATTAGAACCAGATGAGTTAGAATGTGGAAG
CATAAAAATCACATTCTATGATTATAATATTTGATAGTCTAAGAGTTAAGAATCACAGAATGTTGAGAGA
GAAGGAACTCTTGGATGCCATCTATCACAATCCCAACATTTTACACTAGGTAACAATAATCATGTAGTAG
TGGCTCTCAATATAGCCTTGAGCAAGACACATTTTTAAATATGTCTTAAATTTTTACTTTTCTACAATCA
CTACATTAAGATGCATTTTAAAAATGTTTGTCCCAGTGAAATAAAAAATTATAATACTTTGGGAGGACTT
TTGGACAGGCCTCTTGATTCTATATTATATATAATATTTCATATTTCTATGGTATATTACAATTTAAAAG
AATTTTCTCCAAAATAGGCCCATTTACTTCTCACAGTCATCTTTTGAAGTAGGCAGTGAAGTATCATCTC
CACTTTATAAATAAGCACACTAAATCTCAGAATTTGACCACATAAGTAGGTAGAGCCAGAATTAGATTTC
ATTTCTTTCTCATTATAGCATTAACATAGCATCCTTGGAATCAAATATTTTTATTAGTTTCTAAAATCTT
TTGTAAATGTGCAATGAGGGTTAAATGAACAAATGAATAGAGTTTATCATTACTTTTGGTTTCAAACTTA
GATTTCCATGAATTCTACCACCCCCTAAATTATGCACATAATCTCATCTTTCTAACACTCTTTAGTGGGA
AAGGTAGGTCAAGACAATACCAATCCCCTGATATGTCCATCTATTGCGCTTTCAATTCACCACTACAAAT
TCTACAAATTCGGGTTTAAGTTTTTGGCATGAACAATGTGGAGAAATTTTCTTCAGAAAACAGCATCTAG
TATCTTTATGTTGCTGGAGTTGCATTATGTGGCTCTGATTAAACTAGAGTAACATCTGTAAGCTGTCTCC
TTTACATCTTTCAGAAGGGTAGTTAGAAGCAGATTTCTCTTGAATTAATAGAATTTGAGTCAATGGGATT
GCATCTGTGTCCCCTTAAATCCTATTATTTTCTGGGCTTGGATTCACCCTGGCACAAGCTCTTTCTGGCA
CCAGATTTTGTTCATTCTCTCTTAACTGGCACAGCTACTAGGTAGCTTTACACACCTAATGTAGCCTAGG
AGCTTGGATGATTATGCTTATGCTCTATCTGAATCCAAAAACAACCTAATGAAGCTTTTAATCTTGGAAA
ATAAGTCATATAGCATTATAAATCTCAAGTTCTATTGGTAAAAAGATAAAGTCCAAAATTTTACTTTGTA
TTTCTCAGTGAAAATTTTATCTTTAATACTACTAATAATAGCTAATGCTTGTATGGTATTTACCTTATGC
CAGACACGGTTCTGAGTGCTTTACATTTACTTACTAATTCTCACAAAGCCATATTAAGTATATACAGTTA
TTCATTTAAGTAGACATGAAACTGAATCACAAACAGAGTAGGTAATTTGCCAAAGATCATGAAAGCTAGG
ACATGGCAGAGCTGAGATTTCACTTCAGATATCTGGCCCCAGACTCTGTGCTATTAGTGTTATGCTGCCT
CTCACTTAGAAACCTTTCCTAAAACAAGAAAAGATTTTTAAAAGTGGGCTCCTTGGAAATAGATTTTGGT
ATTTTACATACTAAAATGACATAATCAAATATTTTAGAGTTTTACATTTTTAAATGAAACCATAAATGAA
CATTTTTGTTTAGAATTGTCTTATCGATAAAGTGAGAATATTATTTTTATGTTTTCTTCCCACCCCCACC
AACACACAAATATATATTAAAAATATAGGTAAAACTGTTTTAATAGAGGCTTCATTATCAAACTTGGGTT
TTCAAAAAAACAAATTAAAGTTACTCTTTACCTCAGATGAGCTGCTATTAGTCCCATCTGTGCAAATTTT
CATATTACTTTGAATTTTATTAAAACAGAAATTGAACATAAAATATTGTACTTACCTTCTTGGGCATGTA
AAACTTAAATGTGAGCATCCTGGTGAGTTTGGGATTCTTGTAATTCTAAGAAAGTATAATGCATTGTTAT
TAAGAAATGATCTCCAGCTAGATTACTAAATGTAATAATTTTAGTAAGAAAGGAAATATACTTACATTAA
TTCCATTCAAAATCATCTGTAAATCCAGCAGTAAATGCTCCAGTTGTAGCTGTGTTTTCTTTGTAGAACT
TGAAGTAGGTGCACTGTTTGTGACAAGTGCAAGACTTAGTGCAATGCAAGACAGGAGTTGCATCCTGTAC
ATTGTGGCAGGAGTTGAGGTTACTGTGAGTAGTGATTAAAGAGAGTGATAGGGAAC

Protein Sequence : Show Sequence

>gi|28178861|ref|NP_000577.2| interleukin-2 precursor [Homo sapiens]
MYRMQLLSCIALSLALVTNSAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKA
TELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNR
WITFCQSIISTLT

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

28. Il2

Gene Name : Il2
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16183
NCBI Protein GI : 7110653
Locus Tag : DN-144H19.3
Genbank Accession : AF195954
Protein Accession : NP_032392
Taxonomy ID : 10090
Chromosome No : 3
Gene Starting Position : 37120712
Gene Ending Position : 37125953
Gene Strand (Orientation) : -
Protein Name : interleukin 2
Protein pI : 4.63
Protein Weight : 18506.61
Protein Length : 169
Protein Note : Also known as Il-2

DNA Sequence : Show Sequence

>gi|372099107:37120712-37125953 Mus musculus strain C57BL/6J chromosome 3, GRCm38 C57BL/6J
ATTTTTTTTTAGAGGAGAGCTTTATTTCTTGAAAACACTGATTAACATAGAGTTCACAGGAATAACTGAG
AAGTTATTTTAGCGCTTACTTTGTGCTGTCCTAAAAATGACAGACATCTGAGCTTATTTATATTTGAATC
ATCTAAATACTTTTATTAATGAGTCTACCTACACATGATATTTAACAATTCAATATAATAAATAATTTCA
GATAAATAGTTTAAAACATTTTTGAGCCCTTGGGGCTTACAAAAAGAATCTTTAAAGATCCATATTTATC
ATCTGAAGACTAGTAGTTACAAAAGATAGTAAACAATACATCCAAAAATAAATTAAAGTTAAATATTTAA
ATAAATAGAGAGCCTTATGTGTTGTAAGCAGGAGGTACATAGTTATTGAGGGCTTGTTGAGATGATGCTT
TGACAGAAGGCTATCCATCTCCTCAGAAAGTCCACCACAGTTGCTGACTCATCATCGAATTGGCACTCAA
ATGTGTTGTCAGAGCCCTAAAAAAGAATATAAAATTGTAGGCTAAGGTAGCTTACTTTGCATATAATTAT
TCTTCACGGAAGCTTTTAGGCATTTATTTTAACACTGGTTAAATATATTGAACATTCACATTTCTGAAAT
GATCACAATTATTGGGTTATGTTTTATTATGATATCAAATGGTAAAGCATAAAAAATAAAGCTCTCCAGA
GTTGATTACCAACGAGCATTTGATGGGAACACTGAACACTTCTGTAGAATTCAGTTGCTTGACCACTTTT
TCTCTGGTAAGTGTACACAGCTATGCATCAATTTAGGCTTCAGGAATGGAGAAATGATACTTGTTATATG
TTCTTTGCTAAATCTTAAGAGTCTATTATTCAATTCTTTCCCCCCCAAGTCAGAATGGGAACAACCCAGA
GTAGGTTAGGACAAGCATCTATTGGGGGAAATTTGGAGAATTTTGTTATAGATATACCTACCCTGATACC
AGTGTAGATGAATCACAAAGTTTGTACATGTATAAATATTATAAATTTGTTCTGTGGATTAGCTTTTTGA
AAGCTAACCCTCTTTGCTAAAGGAGATATTTTAATTCTGTTTTTAGGTGACAAGCTGAGAAGGAAATATT
TTGACAAATTTATGGGATGGCTTTTCCAAATCTGACTAATTCCTTGTAAGTCAAATGAGGCAGATTATTC
CTTTTAAATAAATACTCCTTTTCAAATAAGCTGCTGTGGTCTTATATACCCTCTTTACATAGCTACTTCA
ATATTCTAATGTCTTGAATTTTTCATTTTTAAAAATATGTGGCCCTTGAAACATTTTTCTAGTTCTCATG
TAGCTCTAAAGTTTGTACCATTCATTAGGGGTGAACTTTCTGATGTTGGCCATAGATTTTAGAGCATCCA
TCTTTGCATACGTAAGTACTCGATTTTAGAGCATCCATCTTAGCATACGTAAGTACTCACACCTTTCAAC
GTCTAGATGATGTGTATTTCAGCCTGCTTTAAAAACCTCAAATGGAAGCTGGGCAGTGGTGGTGCAGGCC
TTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGT
GAGTTCCAGGACAGCCAGGGCTACACAGAGAAATCCTGTCTCAAAAAAACAAACAAACAAACAAACAAGA
AACAAACAAACAAAAAAAACAAAAACAAACAAAACAAAACCTCAAATGGAAACAGTGCACTGAAATGTGA
CTTTGGTTTGGAAAGGACTAGCCCACACCCTCTTGGAGGCTTGCTGCTACTGTCTCACCAGAGTCCATGA
TTCTTGTGCATTTAAAAACAAAGCTCTGCAGTGGGCTCTCCCCGTCCTGCTCTGTCCATTTTAATGGCTG
CCATTTTTGGAGAGAAATGTCTGTTTTTCTACCAATACCAGCACAACTTCTCTGGAAAAACTTTTCAGAT
AATTTTTTCTGATCTGATGAATGTAACACCAGCAAGAGTTGCTTGTTTCTTGTGGAATTCTACTCCGTGC
TTTCTCTCACATCCAGTTCTATGCTGGTGTGGAGGGAGCAGAGTGTTCATGTTCCCAGTTTCCTTGCAGG
TGATGGTAGGTGGAAATTCTAGCATCATCCTACAGTGGAAGGATTCACTTGCACAGTGACTTTAAACTTT
GGCTGACTAAATGCACAGAACCCATCAAAGACCAGAAATGGCAAGCCACTTAAAAATGCATTTCTTCTCT
ATTTTATTTCCAGATTAGCAAATAAAGCAACACCTTACCTTTAGTTTTACAACAGTTACTCTGATATTGC
TGATGAAATTCTCAGCATCTTCCAATTGAAAGCTTTTGCTTTGAGTCAAATCCAGAACATGCCGCAGAGG
TCCAAGTTCATCTTCTAGGCACTGAAGATCTTTCAATTCTGTGGCCTAGAGGAGTAATAAGCTTAACCAT
CAGCTCAGCTCACCACATACTGAAGAGCCAGAAAGTTAGTCTGCAGTCTCTCTAATCAAGAGAAGGCAGC
ACATAGCTTTTACCACTCCCTGTCCTCTGTAAAGGGGGACTGAGGTAATCGATGCCATAAATCTAGAAGA
GCAAGAGCTCACAACCATAAGGGTCACCTTGACTGTTAGGCCACTCTAGTGAGCTCTTCTGGCTTCATTA
GACTTTGTAAAAAGTCTGTGTTTCTCTACCAATGCATAGCACAAGTTCAGACTATTGTTCCAATCTACAA
AGAAATCTGCAAGGTTCACATTCTAATATCTAATCGCAGAGTTGAGAATCACAGAAGGGTAAGGGACAGG
AATCCTTGGATGCCAACTGTCATAGGCCTAAATCTTACAGATTAGGTTATCATGGCCCTGACTGAGGGGT
GTCAAGATAGCCAGGAAGACACATGTAATATTTTTTAAATTTATACTTCTCTGCAATTACTAAAGATGAG
TTTTTTAAAAACCCATATGTCAAGCTAGGAAGATGGCTTTTAAGGTAAAGGTACCTGCCACCAAACTTGA
TGATTTGAGTTCAATTGCAGTGACCCACCCACATTGTGGAAAGAATGGCCCAACTTTCATATTTCGTCTT
CTGACCTCCACAGTTACACTGTGATACACATGCACCTCCACACATACATACATACATACATACATACATA
CATACATACATACATACATACATACATAAAATGTAAAATCTATTTGTCCCAATGAAAAAAGTCATAACAT
TTTGGCAGAACACTTGGGAGAATTTTTCTGATCCATGTATGAAATAATGTTTAAATACATCATGCATACT
TACATACATATACAGGGAAAAAGGATATTGTCTAGCTCTATAGTACACTATGATTTCCATAAAGTCAGCT
GCTTTAAGTCTACAGTGAACTGTGTTATCTCCATTTTACCAAGTCTCACAATCAGTGTCTTTCCCACAAC
CACATGAACGGGGAGCAAGGGTGGCCCGGTCGGTCGGTCGCTCCTTTCATTAACCACAGCCTTTGAATTA
CATGCTTCTATTGTTTTTAAAATCTTACATACCGAATGCAGGTTAAATGAAAACATCAGCAGAATTTACC
ACATTACTCTTGTTTTCAATAATTATTTTAAAACTTAGATTTCTAGGAATTTCACCACTCTCTAAATTAA
CATGATCTCTGTTGTTTCTCTAACAATCCTTAGAGGAAGAGGTTTGACAAGATAACACGTAGTTCCTGAT
TGGTCTGCTATTGACCTTCCAACTTGTTATGGTGTGTCTTGGGTATTCAGTGTGGAGCTCCTTCCCTCAC
AGAACTCTCCAGTTTGTTACTGTGGGAGCTGAACATTATGTGACTGTAATTAAGCTGGAAGAGCAACTGG
GGCCTTTCTTTTTCATCTCCTGTAGAACAGCTAGAGGCAAAGTTCCCCTGACTCAATAGGAATGGGTCAA
CAGCTGCCTCTTTGTCCCCTTAGATCCCATTTTGCTCTAGGCTTGGATTTACTTGGGACAAGCTCTTTCT
AGGACCAAAGTTATCCATTCTCTTTTGACTTTCGGGACTAGAGCAAATTTCTACCCTTGCTGGAGTTCTA
AGAGCTTGTGTAAGCTGTTTTGTATCACACAACATTCTGCCTCCAAAGGAGACCAAAAAACCTTTCAATA
TTGAGAGATAGTATCATGTAGTAATATTACAGCTCTCTAGTTCCACTAGTTAAAAGAGACTGTATAAATC
CAAAATTGTTTTATACATCTCGGACTTCTTCTCTAGCACTAATAGTGTGTTTATGTGCTGTTGGCCAGAC
ACTGTTCTGAAACCTGACAAGTGATAAGCATAGCAATGATCATTTATGTAAGTGGGTGAATGAGCATAGA
CAACTTCCTGAGGCCATGAGAGCTAGGCCCTAGCAGGGCTGGACTCCACTGCAGATCATTGGCCCTAGAA
TCTTAACATTGGTTATGCTGCTCCTCAAAAATGCAGTCATAGGAATCATTCTTAAAACAGTAAAGTGTGT
GTAAAATGAGACCCTCTCAAGTCAATTTTTACATTTAAGAAGCTGAAATAATGCACCTCTCTCAAATATC
TGTAGGACAAGCATAAAACAGAAATCAACAGTCTTTAAATTATCCCAGCATCAAAATGCAATCATCTTGC
ATTTTCTTCTCATTCCCTTTCCCAACACATAAATACACAGGAAAAATGTAAAGAAAACCATTTAAAAGAA
GTACCATTGTCAAGATTTTTTTACAGAGAGATATCAGAGAAACTTATTTTCTTCAGATGAGGTACTATTC
ATCTGGTCACATGTTATTTACACGTTATACACATGTATGTTACTTTGAATTCATTAGAGCACCAGTTAAA
CACAGAAACTCACTCACCTGCTTGGGCAAGTAAAATTTGAAGGTGAGCATCCTGGGGAGTTTCAGGTTCC
TGTAATTCTGAGAAAGCGTAACACATTATTATTAAAGGTTATTAAACACAGCCTTTGGCAAGAAAGCTAA
AGGTATTGCCTATAGATGGGATGGCTGTGCACTTACCTCCATCCTGCTCAGGAGCTCCTGTAGGTCCATC
AACAGCTGCTCCAGGTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGTGCTTCCGCTGTAGAGC
TTGAAGTGGAGCTTGAAGTGGGTGCGCTGTTGACAAGGAGCACAAGTGTCAATGTGACACAGGATGCGAG
CTGCATGCTGTACATGCCTGCAGGACTTGAGGTCACTGTGAGGAGTGATTAGCAAGGGTGAT

Protein Sequence : Show Sequence

>gi|7110653|ref|NP_032392.1| interleukin-2 precursor [Mus musculus]
MYSMQLASCVTLTLVLLVNSAPTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKL
PRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFEC
QFDDESATVVDFLRRWIAFCQSIISTSPQ

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

29. Il4 (interleukin 4)

Gene Name : Il4 (interleukin 4)
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16189
NCBI Protein GI : 10946584
Locus Tag : RP23-188H3.4
Genbank Accession : NM_021283.1
Protein Accession : NM_021283.1
Taxonomy ID : 10090
Gene Strand (Orientation) : ?
Protein Name : IL-4, Interleukin 4

DNA Sequence : Show Sequence

>gi|10946583|ref|NM_021283.1| Mus musculus interleukin 4 (Il4), mRNA
GGATCCCCGGGCAGAGCTGGGGGGGGATTTGTTAGCATCTCTTGATAAACTTAATTGTCTCTCGTCACTG
ACGGCACAGAGCTATTGATGGGTCTCAACCCCCAGCTAGTTGTCATCCTGCTCTTCTTTCTCGAATGTAC
CAGGAGCCATATCCACGGATGCGACAAAAATCACTTGAGAGAGATCATCGGCATTTTGAACGAGGTCACA
GGAGAAGGGACGCCATGCACGGAGATGGATGTGCCAAACGTCCTCACAGCAACGAAGAACACCACAGAGA
GTGAGCTCGTCTGTAGGGCTTCCAAGGTGCTTCGCATATTTTATTTAAAACATGGGAAAACTCCATGCTT
GAAGAAGAACTCTAGTGTTCTCATGGAGCTGCAGAGACTCTTTCGGGCTTTTCGATGCCTGGATTCATCG
ATAAGCTGCACCATGAATGAGTCCAAGTCCACATCACTGAAAGACTTCCTGGAAAGCCTAAAGAGCATCA
TGCAAATGGATTACTCGTAGTACTGAGCCACCATGCTTTAACTTATGAATTTTTAATGGTTTTATTTTAA
TATTTATATATTTATAATTCATAAAATAAAATATTTGTATAATGT

Protein Sequence : Show Sequence

>gi|10946584|ref|NP_067258.1| interleukin 4 [Mus musculus]
MGLNPQLVVILLFFLECTRSHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCR
ASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS

Molecule Role : Vaximmutor
Molecule Role Annotation : IL-4 plays an important role in Th2 immune response.
Related Vaccine(s): Mycobacterium tuberculosis fadD26 mutant vaccine , Mycobacterium tuberculosis mce-2 mutant vaccine , Mycobacterium tuberculosis mce-3 mutant vaccine , Mycobacterium tuberculosis sigE mutant vaccine

30. IL6

Gene Name : IL6
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 3569
NCBI Protein GI : 10834984
Genbank Accession : AC073072
Protein Accession : NP_000591
Taxonomy ID : 9606
Chromosome No : 7
Gene Starting Position : 22766765
Gene Ending Position : 22771620
Gene Strand (Orientation) : +
Protein Name : interleukin 6 (interferon, beta 2)
Protein pI : 6.52
Protein Weight : 22923.52
Protein Length : 212
Protein Note : Also known as HGF; HSF; BSF2; IL-6; IFNB2

DNA Sequence : Show Sequence

>gi|224589819:22766765-22771620 Homo sapiens chromosome 7, GRCh37.p5 Primary Assembly
CAATATTAGAGTCTCAACCCCCAATAAATATAGGACTGGAGATGTCTGAGGCTCATTCTGCCCTCGAGCC
CACCGGGAACGAAAGAGAAGCTCTATCTCCCCTCCAGGAGCCCAGCTATGAACTCCTTCTCCACAAGTAA
GTGCAGGAAATCCTTAGCCCTGGAACTGCCAGCGGCGGTCGAGCCCTGTGTGAGGGAGGGGTGTGTGGCC
CAGGGAGGGCTGGCGGGCGGCCAGCAGCAGAGGCAGGCTCCCAGCTGTGCTGTCAGCTCACCCCTGCGCT
CGCTCCCCTCCGGCACAGGCGCCTTCGGTCCAGTTGCCTTCTCCCTGGGGCTGCTCCTGGTGTTGCCTGC
TGCCTTCCCTGCCCCAGTACCCCCAGGAGAAGATTCCAAAGATGTAGCCGCCCCACACAGACAGCCACTC
ACCTCTTCAGAACGAATTGACAAACAAATTCGGTACATCCTCGACGGCATCTCAGCCCTGAGAAAGGAGG
TGGGTAGGCTTGGCGATGGGGTTGAAGGGCCCGGTGCGCATGCGTTCCCCTTGCCCCTGCGTGTGGCCGG
GGGCTGCCTGCATTAGGAGGTCTTTGCTGGGTTCTAGAGCACTGTAGATTTGAGGCCAACGGGGCCGACT
AGACTGACTTCTGTATTTATCCTTTGCTGGTGTCAGGAAGTTCCTTTCCTTTCTGGAAAATGCAGAATGG
GTCTGAAATCCATGCCCACCTTTGGCATGAGCTGAGGGTTATTGCTTCTCAGGGCTTCCTTTTCCCTTTC
CAAAAAATTAGGTCTGTGAAGCTCCTTTTTGTCCCCCGGGCTTTGGAAGGACTAGAAAAGTGCCACCTGA
AAGGCATGTTCAGCTTCTCAGAGCAGTTGCAGTACTTTTTGGTTATGTAAACTCAATGGCTAGGATTCCT
CAAAGCCATTCCAGCTAAGATTCATACCTCAGAGCCCACCAAAGTGGCAAATCATAAATAGGTTAAAGCA
TCTCCCCACTTTCAATGCAAGGTATTTTGGTCCTGTTTGGTAGAAAGAAAAGAACACAGGAGGGGAGATT
GGGAGCCCACACTCGAATTCTGGTTCTGCCAAACCAGCCTTGTGATCTTGGGTAAATTCCCTACCACCTC
TGGACTCCATCAGTAAAATTGGGCGTGGACTAGGTGATCTCATAGATCCTTCCTGCTGGAACATTCTATG
GCTTGAATTATATTCTCCTAATTATTGTCAAAATTGCTGTTATTAAGTATCTACTGTGTGCCAGGCACTT
TAAATAAATATTGTGTCTAATCTTCAAAACAAATTTGCAAGGAAGGTTTTTGGAGATAAGGAAACTGAGA
CTCAGGATTAAGTAACACACCTAAAGTCACAGGTGAGCTTGGAACTGAACCCAAGTGTGCCCCCACTCCA
CTGGAATTTGCTTGCCAGGATGCCAATGAGTTGTAGCTTCATTTTTCTTAGAGACTTTCCTGGCTGTGGT
TGAACAATGAAAAGGCCCTCTAGTGGTGTTTGTTTTAGGGACACTTAGGTGATAACAATTCTGGTATTCT
TTCCCAGACATGTAACAAGAGTAACATGTGTGAAAGCAGCAAAGAGGCACTGGCAGAAAACAACCTGAAC
CTTCCAAAGATGGCTGAAAAAGATGGATGCTTCCAATCTGGATTCAATGAGGTACCAACTTGTCGCACTC
ACTTTTCACTATTCCTTAGGCAAAACTTCTCCCTCTTGCATGCAGTGCCTGTATACATATAGATCCAGGC
AGCAACAAAAAGTGGGTAAATGTAAAGAATGTTATGTAAATTTCATGAGGAGGCCAACTTCAAGCTTTTT
TAAAGGCAGTTTATTCTTGGACAGGTATGGCCAGAGATGGTGCCACTGTGGTGAGATTTTAACAACTGTC
AAATGTTTAAAACTCCCACAGGTTTAATTAGTTCATCCTGGGAAAGGTACTCTCAGGGCCTTTTCCCTCT
CTGGCTGCCCCTGGCAGGGTCCAGGTCTGCCCTCCCTCCCTGCCCAGCTCATTCTCCACAGTGAGATAAC
CTGCACTGTCTTCTGATTATTTTATAAAAGGAGGTTCCAGCCCAGCATTAACAAGGGCAAGAGTGCAGGA
AGAACATCAAGGGGGACAATCAGAGAAGGATCCCCATTGCCACATTCTAGCATCTGTTGGGCTTTGGATA
AAACTAATTACATGGGGCCTCTGATTGTCCAGTTATTTAAAATGGTGCTGTCCAATGTCCCAAAACATGC
TGCCTAAGAGGTACTTGAAGTTCTCTAGAGGAGCAGAGGGAAAAGATGTCGAACTGTGGCAATTTTAACT
TTTCAAATTGATTCTATCTCCTGGCGATAACCAATTTTCCCACCATCTTTCCTCTTAGGAGACTTGCCTG
GTGAAAATCATCACTGGTCTTTTGGAGTTTGAGGTATACCTAGAGTACCTCCAGAACAGATTTGAGAGTA
GTGAGGAACAAGCCAGAGCTGTGCAGATGAGTACAAAAGTCCTGATCCAGTTCCTGCAGAAAAAGGTGGG
TGTGTCCTCATTCCCTCAACTTGGTGTGGGGGAAGACAGGCTCAAAGACAGTGTCCTGGACAACTCAGGG
ATGCAATGCCACTTCCAAAAGAGAAGGCTACACGTAAACAAAAGAGTCTGAGAAATAGTTTCTGATTGTT
ATTGTTAAATCTTTTTTTGTTTGTTTGGTTGGTTGGCTCTCTTCTGCAAAGGACATCAATAACTGTATTT
TAAACTATATATTAACTGAGGTGGATTTTAACATCAATTTTTAATAGTGCAAGAGATTTAAAACCAAAGG
CGGGGGGGCGGGCAGAAAAAAGTGCATCCAACTCCAGCCAGTGATCCACAGAAACAAAGACCAAGGAGCA
CAAAATGATTTTAAGATTTTAGTCATTGCCAAGTGACATTCTTCTCACTGTGGTTGTTTCAATTCTTTTT
CCTACCTTTTACCAGAGAGTTAGTTCAGAGAAATGGTCAGAGACTCAAGGGTGGAAAGAGGTACCAAAGG
CTTTGGCCACCAGTAGCTGGCTATTCAGACAGCAGGGAGTAGACTTGCTGGCTAGCATGTGGAGGAGCCA
AAGCTCAATAAGAAGGGGCCTAGAATGAAACCCTTGGTGCTGATCCTGCCTCTGCCATTTCTACTTAAGC
CAGGGTTTCTCATATGTTAACATGCATGGGAATTCCCTGGGCATCTTCTTGTGGTGTGGAGTCTGACTTA
GCAAGCCTCGGGTGGGTTTGAGGGTCAAATTTCTACCAGGCTTATATCCCTGGTGATGCTGCAGAATTCC
AGGACCACACTTGGAGGTTTAAGGCCTTCCACAAGTTACTTATCCCATATGGTGGGTCTATGGAAAGGTG
TTTCCCAGTCCTCTTTACACCACCGGATCAGTGGTCTTTCAACAGATCCTAAAGGGATGGTGAGAGGGAA
ACTGGAGAAAAGTATCAGATTTAGAGGCCACTGAAGAACCCATATTAAAATGCCTTTAAGTATGGGCTCT
TCATTCATATACTAAATATGAACTATGTGCCAGGCATTATTTCATATGACAGAATACAAACAAATAAGAT
AGTGATGCTGGTCAGGCTTGGTGGCTCATGCCTGTATTCCCTAAACTTTGGGAGCCTAAGGTGAGAACTC
CTTGAACTCCTAAGGCCAGGAGTTCAAGACCAGCCTGGATAACATAGCAAGACCCCATCTCTACAAAAAA
CCAAAACCAAACAAACAAAAATGATAGTGGTGCTTCCCTCAGGATGCTTGTGGTCTAATGGGAGACAGAA
CAGCAAAGGGATGATTAGAAGTTGGTTGCTGTGAGCCAGGCACAGTGCTGATATAATCCCAGCGCTATGG
GAGGCTGAGGTGGGTGGATCATTTGAGGCCAGGAGTTTAAGACCAGCCTGGTCAACATGGTAAAACCCCA
TCTCTACTTAAAAATACAAAAAAGTTAGCCAGGCATGGTGGCATACACCTGTAACCCAGCTACTCAGGAG
GCTGAGGCACATGAATCACTTGAACCCAGGAGGCAGAGGTTGCTGTGCACCACTGCACTCCAGCCTGGGT
GACAGAACGAGACCTTGACTCAAAAAAAAAAAAAAGAAGTTTGTTGCTATGGAAGGGTCCTACTCAGAGC
AGGCACCCCAGTTAATCTCATTCACCCCACATTTCACATTTGAACATCATCCCATAGCCCAGAGCATCCC
TCCACTGCAAAGGATTTATTCAACATTTAAACAATCCTTTTTACTTTCATTTTCCTTCAGGCAAAGAATC
TAGATGCAATAACCACCCCTGACCCAACCACAAATGCCAGCCTGCTGACGAAGCTGCAGGCACAGAACCA
GTGGCTGCAGGACATGACAACTCATCTCATTCTGCGCAGCTTTAAGGAGTTCCTGCAGTCCAGCCTGAGG
GCTCTTCGGCAAATGTAGCATGGGCACCTCAGATTGTTGTTGTTAATGGGCATTCCTTCTTCTGGTCAGA
AACCTGTCCACTGGGCACAGAACTTATGTTGTTCTCTATGGAGAACTAAAAGTATGAGCGTTAGGACACT
ATTTTAATTATTTTTAATTTATTAATATTTAAATATGTGAAGCTGAGTTAATTTATGTAAGTCATATTTA
TATTTTTAAGAAGTACCACTTGAAACATTTTATGTATTAGTTTTGAAATAATAATGGAAAGTGGCTATGC
AGTTTGAATATCCTTTGTTTCAGAGCCAGATCATTTCTTGGAAAGTGTAGGCTTACCTCAAATAAATGGC
TAACTTATACATATTTTTAAAGAAATATTTATATTGTATTTATATAATGTATAAATGGTTTTTATACCAA
TAAATGGCATTTTAAAAAATTCAGCA

Protein Sequence : Show Sequence

>gi|10834984|ref|NP_000591.1| interleukin-6 precursor [Homo sapiens]
MNSFSTSAFGPVAFSLGLLLVLPAAFPAPVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKE
TCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQA
RAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALR
QM

Related Vaccine(s): Bacille Calmette-Guérin (BCG)

31. lipY

Gene Name : lipY
Sequence Strain (Species/Organism) : Mycobacterium tuberculosis H37Rv
NCBI Gene ID : 888677
NCBI Protein GI : 57117054
Locus Tag : Rv3097c
Genbank Accession : BX842581
Protein Accession : YP_177924
Taxonomy ID : 83332
Gene Starting Position : 3465777
Gene Ending Position : 3467090
Gene Strand (Orientation) : -
Protein Name : triacylglycerol lipase
Protein pI : 4.26
Protein Weight : 40955.98
Protein Length : 437
Protein Note : 3.1.1.3

DNA Sequence : Show Sequence

>gi|57116681:3465777-3467090 Mycobacterium tuberculosis H37Rv chromosome, complete genome
ATCAGGCGGCGATACCGAGTTGCTGGTTAATCTGCGGCCAGGACAGCAAACCCCAGGGGGTGAGCAGTAT
CCAGTCGTGGATTTGCCAGGGGGCCAGTACGAAGCTGAACGGCGCTCCTTGGACTACGGCTGTGTGCTCG
AGGACAACCGCTTGTTGTGCGAGCGGATCAAGCGAGCCCGAATAGACATACGTCGGCGGAAGACCGTTCA
GCGACCCATACAGCGGACTGACCAGCGGGTCGTTGACCGCAAGATTGCCTGCCCACGCCTGGCTGATCTG
CCAGGTCCCCACATCGAGCCACGGGGACAGCAACACCATGGACGACGGTACTGGGTTGCCCTGGCTCACC
ATGTATTGGGCGGCCGCCAGTGCGAGGTTGCCGCCCGCGGAGTCCCCGACCACGCTGACGTTGGAGACCC
CGTGTTGCGCGATTTGCGTGGAGATGAGCCCGGCCATCGCCGGTACTACCGTCCCGGCAGTGCCTCCTTC
CTGCACCAACGGGTAAATCGGCACTTGCACGGTCGCGCCGGTCTGGTAAGCCGTCACCGAGTAGTTGAGC
CAGTGGAAGATTGACGGCGGCAGGATAAACGCGCCGCCGTGAATGGCAACCACGTATTCGCCGGTTGGAT
GAGCCGGCGTGATCTGCACGACGCTCATCCCGTCATAGGTGGTGTACTGGACCGTCTGTCCCAGCAGCGA
GTTCAGCAACGGCGGTGGGGAGTTGCCAAGAAACCACGACAGCGGCGGTATGTCGCTGGCAATGAGCGCT
AAAAGTGGATTGTTTGGGATTGCAAAGTGAGTTTCGAGCGCAGACAAACTCAGCAGGGGTTTCACCGGCC
ACAGCGAAGCGATGTCGAATCCGGCAGCCCCTGACGGCGTGCCGGTGAAGATCCCTGCCTGCGCCGCCGC
GAAAGGCGGAACCTGCGTGAATCCGGCGGCCAGCGCCGTGGGGGCCCGCTGAATTTCCTGGTGAATCGTG
GCAAAACCGTTCCCGATACCGCTGGCGAATTCACTCTGCAACAGCGAAGCGTTGGCCAGCTCGGCGGCGG
CATACCCCTTGGCCGCGCCTGTCAATGCCTGCACAAACCGCTCATGAAACACCGCAAGCTGTGCGCTAAG
AGCTTGATAGTCCTGACCATGGCCGGAAAACAAAGCCGCGATCGCGGCTGACACCTCGTCCTCGGCAGCG
GCTAATACCGTCGTGGTGGCACCCGCGACACCCTGGCTCGCCGTCGCGACCACCGAACCAATCGAAGCCA
CGTCTGTGGCCGCGGCGGACATCACCTCCGGCAACGCAACAACATAAGACACCA

Protein Sequence : Show Sequence

>gi|57117054|ref|YP_177924.1| lipY gene product [Mycobacterium tuberculosis H37Rv]
MVSYVVALPEVMSAAATDVASIGSVVATASQGVAGATTTVLAAAEDEVSAAIAALFSGHGQDYQALSAQL
AVFHERFVQALTGAAKGYAAAELANASLLQSEFASGIGNGFATIHQEIQRAPTALAAGFTQVPPFAAAQA
GIFTGTPSGAAGFDIASLWPVKPLLSLSALETHFAIPNNPLLALIASDIPPLSWFLGNSPPPLLNSLLGQ
TVQYTTYDGMSVVQITPAHPTGEYVVAIHGGAFILPPSIFHWLNYSVTAYQTGATVQVPIYPLVQEGGTA
GTVVPAMAGLISTQIAQHGVSNVSVVGDSAGGNLALAAAQYMVSQGNPVPSSMVLLSPWLDVGTWQISQA
WAGNLAVNDPLVSPLYGSLNGLPPTYVYSGSLDPLAQQAVVLEHTAVVQGAPFSFVLAPWQIHDWILLTP
WGLLSWPQINQQLGIAA

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

32. Ltf

Gene Name : Ltf
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 17002
NCBI Protein GI : 31560677
Genbank Accession : AC118727
Protein Accession : NP_032548
Taxonomy ID : 10090
Chromosome No : 9
Gene Starting Position : 111019291
Gene Ending Position : 111042765
Gene Strand (Orientation) : +
Protein Name : lactotransferrin
Protein pI : 8.61
Protein Weight : 73470.01
Protein Length : 707
Protein Note : Also known as Lf; Csp82; Ms10r; MMS10R

DNA Sequence : Show Sequence

>gi|372099101:111019291-111042765 Mus musculus strain C57BL/6J chromosome 9, GRCm38 C57BL/6J
CAGGCTTGTCCTCTAGGTCTCCCAAGACCACAGACATGAGGCTGCTCATCCCTTCCTTGATATTTCTTGA
GGCCCTTGGTAAGTGCAGGTGCCCAGAGGTAGGGAGGGGCGGGAGGTCACCTGCTGGGAAGCTGGGCACT
AAATGCACTCTTCTGTTATCTAGGGAAGACTGTTGGGCTCAGAGAGGGTGAGATGTTCCTGTGAGTCCCA
GTTCACTGCCTCCCAAGAAGGCTTCTTTGTACCAACATTGGGGACCCTCCATGTTTGACTCTATGCCCTC
TCTTAGTCCTCCTGCTGCTGGACTCCACATCTAACCCAGGCATCCTATACAGGCAGGCTAGCTCAGTGGC
CAGTGGTGCTGGTGCCCAACCGCCTAGGTTCAAATTCTAGAGTCAACACAACTTAGCTGGAAAGTCATAT
AACTTGGTAAAGCTACACAATGCCTAGAACTATGGGGGTATTGACAAAAATCACACTGAAGGATAGATGG
GGTGACCCAGTGAGTAAAGGTGCTTGCTACCAAGACTGAAGACAGAAGTCCTAGTCCCAGAACTCACTCA
ATGGAACTACCAGCTTTCTATCTATAAATTTATCTTTTCTGGTCGAGTGTGGTGGTGCACACCTTTGATT
CTAGCACTTGGGAAGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGC
AGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAG
GCAGAGGCAGGCAGATCTCTGTGAGTTCAAGGTCAGCCTGCTCTACATAGTGAGTTCCAGGATAGCCAAG
GCTACGTAGAGACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTGGAGTTATCCAATATTTTGTCTT
GGATTTGGCTTCCTTCATCTAGTCATGTTATATGTATGTGTATTATTTTTTATATAATTGAAGAATATTC
CATTGAATGGATGTACTGCATTTTCTTCAGCCATTCCTATTCACCAGTGTTTGGGATTTATGTAGTTAGT
TGGGTTTTTTTGTTGTTGTTGTTATTTTGAGACCTTTTTCCTATCTAAATAATGCTACTATGAATATTAA
TGCTAGATCTTTAAGCAGATTTATGTTTTTATTTCTATGAGCAATTGCCAACCTGTTTCCCAAAGTGGCT
ACATCATTTTACAGTCTCAGCAATGTAAGAAGGTTTCCATGTTTGCAACATTAAGATCCAGTGTGCACAT
GAGTAAAACATGAGCCACGCCATTTCCCTGCTTCCCTGCCAGGTTCATCTAGGACAAGGCTGATGGAGGT
CAATAAAATGATCTTTGGAGTGGATCATCTTTGGGGGTGGGGTGGGTGTCTGAGTCTCATCCCTCTGTGG
TCCAGCAGCTTAGCTTAACTAGAGTACAGTGGCTGAACAAGACCACGTAACACGGAGGGTGGGGCAGTGA
CCACGGGCAGTCTACAGAATGCCCTCAGACATCGGGGAGGTCTTCCCCAACGAGCACTGCTGGATTCCAT
TCTGTCTTTCTCCCAGGACTCTGTCTAGCTAAGGCAACAACTGTTCAATGGTGTGCTGTGTCAAATTCTG
AGGAAGAAAAATGTTTAAGGTGGCAGAACGAGATGAGAAAAGTGGGTGGCCCGCCGCTCAGTTGTGTCAA
GAAATCCTCCACCCGCCAGTGCATCCAGGCCATTGTGGTAAGCTAACACTCCAACCCGAGTGGAGGAAAG
CAAGGAGAGGTGCGCAGTCCAACCACAAGTTCGAGTCTCTGGTCCCTCTGCTTCACTTCCTGGGCACCAG
TCAGTTGAGAGCAGTCTTTGCTCACAGAAACCGGTGTCTCTTGATATCTAAAGAAAGACAAATGACAGGC
CTCTTATGGGGTAACTGGGGTCTTATTTCAAATTGTTCATAGGAACTATTTTTTTAAAAGCCTAAGCAGC
CATATTTGATGAATATGTAATGTCTCTAATCCCTTGAGAAATAAGCCTGGTGCAGCCCTAGATGCCATAT
CCCAACTGTTGGGTAGGGATGGCTAGGACTCCAGACATTAAAAAGACACTTGTTTGCCCTCCAGCACCTA
TGTTAAAACCTAGAGATAGTAGCTTTCCCACTTGGACTCTGGGTCTAGGAAGTAGAGACTGGAGGTCTAA
ACAAACTGGTGAGCTCCAGGTTTGGCAAAAGACTTTGTCTCAAAACGTAAGTCAGAAAGAGATGGAGGAA
GACACCAGAAGTCGAGCTCTGGCCTCCACACGTTCATGTCCTCCAGTGTGTGAGCACACACACACACACA
CACACACACACACTGCGCACATCTACAAGACTGAAAATACACATGAAGGCAAAATAGGTGATAAAGTATG
TGGATCAAAAAGCTTTAAATACAGAAGATTGTGACAGTGTGACCAAGAGTAACTGAAGTGGACAGGCATA
CGTAGGGGTCACACACAACTTCAGGAGTTAAAGCCCTGGGGGTGCTTACTGCTCACATCGAATGACCCCT
GACCTGTGCTGTTGTCATCTTCTAGGTAGGACATATGGACAATACGAAGGGCTAACTCATTCTGAAGTCC
TGAAATCTTTTCTCCTGAAGCATGTCTGGACCCATTTTCTTTCAGACAAACAGAGCTGATGCCATGACTC
TTGATGGTGGCACTATGTTCGATGCAGGAAAGCCCCCCTACAAACTGCGACCTGTGGCAGCTGAAGTCTA
CGGGACCAAAGAGCGTGAGTTCTTTTTCTGTGGACGCTGAAACCTCGTGACTGTGTTGTGGCTATGAAAT
GGTTTCTGTGGCGTGGGAGTGGACGAGTAAACCTAGTCAGTGCCTCTCACTGGGGAACAGAGTCTCTGGA
CTCTGGATGACAGGTCCTCCTGGTGCTTGGGTCTCCTTGCCATGGCAGCTTGGGCAGAGTGGATTCTTCA
CCTCCCTCTCATCGGGGCTAAGCCTGTCTAGTGCCTCTGTTCCTCTGCAGAGCCCCGGACTCACTACTAT
GCGGTAGCAGTCGTGAAGAACAGCAGTAACTTTCATCTGAACCAACTCCAAGGCCTGAGGTCCTGCCACA
CCGGCATTGGCAGGAGTGCGGGGTGGAAAATCCCTATAGGGACACTTCGTCCATACCTGAATTGGAATGG
GCCACCTGCATCCCTTGAGGAAGGTAAAATGGCTTGTGGGGGAGCGGGGAGAGATCCTGAGTGGTCCAAG
CTGGGAATTTCTAGTCTCACTGTAGACACAAGCCACTTTGGGATGGCTTTCCATATGGGATGAGTCCAAG
GTTGGGCAGAGGGAGTCTAATCAAGTAGAAGCAGGGGCCATCACACCAACAACTCCGGGAGCCGCCAGTA
GCTGTTCTCTGGGCAAAGATGCACGTCTGAGTTCCGAAGCCCACTCAGCTCTGTCCTGTTCTGCCTGGCG
GAGGAGGGGGCTCTTGGTCCGTGGAGGGTGGAGGGGTGGCTGCTCCTGGGAGACAGGACTGCAGTGGCTG
GGATCGGCTCATCCTCTGTGGCCCACTTCTCTGTGTTTGACAGCGGTATCCAAGTTCTTCTCAAAGAGCT
GTGTTCCCGGTGCCCAAAAGGATAGATTCCCCAACCTGTGTAGCTCGTGTGCAGGGACAGGAGCCAACAA
ATGTGCCTCTTCCCCCGAGGAGCCATACTCAGGTTATGCTGGAGCCTTGAGGTGAGCGACACCATTCTCT
CCTCCCCCACGGCCCATGGTCGCACAGCTTCAGGCCAAGGTGCGGGATGGAGATTTGGTCCTACTGATGT
GCAGACGGAGAACCCAAGCCTAAGAAGTGGTTGGTCTCTCTACTACATGTCCTTCTCCAGCCCCTTAGAG
GCCCAACAAGGCCCCAAAGTCTATATCACTTTGATGGAAGCTAACCTGGTTATGGCCCCTCTGATCACCT
CACGCCTGATTTTGCTCTCCTATTTTTTGGGGTTATCCTGCTAACTTTCTCCCTGTTTTCATCCCATCTG
GGTGGGGCAGCCATTGTGGCCTTGATATTTAGTCCAACTCAGGCCTCTCTCTCGAGTAGGAGAGGGTGAC
CTCTGTCTGACTTCCTCCTCTGGCCTCTATGCTGTTGCCTAGGCTCAGCACCTCCTCTCTAGTCCTTTCC
TGAAGCTCAGGCACTTTTGTAGAGTGACTTCCCTAGGTTCTTGCTGGCCCTAGCACATTTGCCCACTCAG
CATGGAGAGAAAGAGCCCCATGGTCGGGGACACCTCATCTTTAACTGCGTTCTTCTCATTTCACAGGTGT
CTGAGAGACAATGCTGGAGATGTGGCTTTTACCAGAGGAAGCACGGTATTTGGTAAGGGCAAGGGTCAGG
CTGTGGGTGTGGCCACCTCTTAAGTGCTTTATCTGTGATGTGCGGGTCACCAGATTCCCCAGTTGTGATC
AAGTTGCCTACTGGGTCCAACTCTTCTTTTAGGTTTTTCTTTTAAAAAAATACTTTAAGATTAATTATGA
TTTTACGTGCATGGGTGTTTTGCCTTCGTGTATGTCTGTGCACCACATGTGTGCCTGATACTCAAGGAGA
CCAGAAGAGGGTGTCAGATCCCTTGGGACTGGAGCTACAGGTGGTTGTGAGCTGCTATGTGGGTGCTGGG
AAGTGAACCCAGGTTTTCTGTGAGAGTAACCACTGAGCAACCTCTCCAGCCCCTACCTCATGCTCTGAAA
TCTCCCACTTCTACCCCCTTCTCCTTCCCCTCACAGACACTCTCACCAGGGAGTTCGTCATGTGCTTGCC
CAGGTGTACACGCCTCTGCACCATAAACTTCACTGGCTTGTGTCTCTGGATTCTGAGTTCACACAGCTTT
TACTGAACACTAGAAGCAGCGCGAGCCCAGGGCTTTCCACTCAGCATTGGGGGTCCAAGATTGATCCGTG
GTTTTACTACACGAGTAACTCCGTGGTTCTGACAGTAGAAGAACGATCCGTGGAACATGTCTACCATATT
TAATTAACGTCTCTGTCAGTCTAATGACAGACAGACAGGCGCGGCGGTCCTCCCTCCTTTCGGCTTTACC
TTTTTGAGTGCTCAGGAAGAGTGCTCATCTTCACACGTTTCCCTTCCTCCCAGAGGAGTTACCAAATAAA
GCCGAAAGGGACCAGTACAAGCTGCTCTGCCCAGACAACACCTGGAAGCCGGTGACAGAATACAAGGAGT
GCCACCTGGCCCAAGTCCCTTCACATGCTGTCGTATCCCGAAGCACGAATGACAAAGAAGAGGCCATCTG
GGAGCTTCTCCGCCAGTCACAGGTACAGCTACAAGTGCACAGCACCCCTTGCCTGCTTAGGTTTAGGGGT
GGGGAGGGCCTCTCTTTCCCTAACTTCAGCAGTTGAGAGAGTTCCATAGTTTAAGGACCATGGGCCCTGA
CTCTCCCTTGGACAGTAGAGACATAGGAACCTTCTTCAGTGTCAGCCCCTAGCAGGCTTTCCTGGGCTTC
CGCAGAGGCAGGGGTGGGTACCGGATGCTATTCTTAGGTCCCCTAAGGACCTTGGGACAAAACAGTTTTC
TGATGAGGTAAGTTATCGTTGAGCTCATGTCTCTGCAATGAGCAGAGTCCCTGGGAGCTCGGATATCAAT
TCCATTTGAATTGTCCCCAGATCCCAAGTAGCTGTTTCCCACACTGGGTTTCGATTCCTTCCTGCCTACT
GCTGGTGACCATAATTCTATTTTCTTTCCTTAAACAGGAGAAGTTTGGAAAAAAACAAGCATCGGGATTC
CAGCTCTTTGCCTCCCCCTCGGGACAGAAGGACCTGCTGTTCAAGGAGTCTGCCATTGGCTTTGTGAGGG
TTCCCCAGAAGGTAGATGTAGGGCTCTACCTGACCTTCAGCTACACCACATCCATACAGAACCTGAATAA
AAGTAAGTGGACCCCAGGCAGGAACCCAAGGGTACTGAGCCAGCAGTACCTCTGGGTGGGTGACCTCTGC
TCTGGGAGGGTCTCTCCGCTACTGGTGGGGCTGACGCACATACTGTGTGTCAAGTAAGTCAAGGCCATTG
TGAAAAATGTCTCCAAAAAAAGTCCCAGACGCTGGACTAATGCTTCCATATTCCTCTGTGTATGCCAGGA
TCCCAAGGCCTCTTATTAAGTGAGAAAGAGAGGCATGGACTAAGAAAAGAAGAATCTGGGGAGATTACTC
CAGAGGCTAGGTGAAGCCCCCCTTCTGTGCTGGGATTCGAACTTGGCTGAAGAGATTACAATGGGCTGTA
GAGATGACAACTTTGGCTATGTTTATCCTACTTGGGAGGACTTAGAGAGGGAGTCTGAGTGAACCAGGAG
ACTGTCCTGTGACATCTGCTGGTGACAGAGCCTTATGGGTGGGTGGTCGGGCTCAGTCAAGGTCCTCTAA
GCCAGCCTTTGGGGTATACACTAGTTTCCATGTCATGAAGCTTTTATTCTGAGGGACAGAACATTAGGCA
CTCCACTCATTACACCAAGGACTGATGGATGAATTAGAAGCCAGGGAAGCCCTCTGAAAGAGCAGAAGGA
GAAACAGAAAGCTTCCGGCCAAATCCAGTTTGAGGGCTGTGATGCGGGGAAAGAGAAGGTCTCACACAGA
TTCCTCGGTCTCAGAAGATCTGGGCAGGACCTTGATCCCCAAGGAGGGCAGAGGTGGGAGTGAGGCAGCG
CCTGAGTGTTCCTCACCACTGTCCTTCCCCACAGAGCAGCAGGATGTGATAGCCTCAAAGGCCCGGGTCA
CATGGTGTGCCGTGGGCAGTGAGGAGAAGCGCAAGTGTGATCAGTGGAACAGAGCAAGCAGAGGCAGGGT
CACCTGCATCTCATTCCCCACCACGGAAGACTGCATTGTCGCAATCATGGTAGGGAGCCCGGGTCACACG
TGCTGTGGGTGGTGGGCTTCTTAGAGGGCACGGACCATGGTCATCTACTTATAGTGAGGTTGGTCACCCG
AGAGGCCACTGTGAAGAAGGAACACCCAAAAGAGAAGGTGGGCTCGGGACCACTCATGCACATGTGTTGC
ATGAAGGAGCGGCCTTCTAGGTTTGAAAACCAATTTGTTGTGGAATTTCTGCCCTTTCCAGTAAAAGTAC
AATTTAGGGTTATTAGGTTCATTTGGAAAATATGGAACAATTTTAGCATCTCTCTCTCTCTCTCTCTCTC
TCTCTCTCTCTCTCACACACACACACACACACACACACACACACACACACACACACCTCTGCATGCAACT
TCATATAGAAATATTTACAGTGAGAGTTTTGAAATTCCCCACCCCAGGTTTCTCTGTCCATGTAGGCCGA
GCAATTCTGGATTTGTATGGGTTATTCTCTTTTGATCCCTCGTTCAAATAAGCAGTATACCATGGCTACT
TCCCCACATCAATGCTAATCTCGGGAAACAGTTTTAAATGCTTATTTTATTTTATGTATATGTGTGAGTG
TCTGAACGTGTATGGGAGCCACATGCATGCAGGTGCCAGAAGAGGGTGTTGGATCCTCCAGAAGTAGCGT
TAGAGACAGTTGGAGCTGCCATGTTGGTGCTGGAACTGAATTCAGGTTCCCCGAAAGAGCAATGGACGCT
CTTAGCCACTGAACCAAATCTCTTGTCCCCTCTCATCATTTTTAACGCCCTTGTATAGCAGTGCTGACTG
GGTAAAGGAGCGTGCAGTCCTGATAAACTCGTGTTTCTGCGAGGGTTGTGGCTTGTTCATTCTCATGAGT
CAACTTGATACCCAATCTCTAGGTACCACGTTGAGTATCGTGCTAAGAAGCAGAAGTTAGCTGAAAGCAG
CTTTTCTCAGCGTGTTTTCTGTGAGTGAGGCTGGTTCAGAAAGGAGTGCCAGGAGTGCTCGGGTACAGGA
AGAGTTCTGTTCAGAAGACAGAGTTAGTGTCTCAGGTGTGTGGGTGACAGCCCTAAGATAGTGAGACCGG
CGGAGGACTGAGTCCATGTGGTATTTCCTGGGGGTGTTACGTGCAAACAACACGTGTCTTGAGGCCATCC
CTGAGAGCTCCGAAGACAGCATCTTTGTGTGGTGTGTGTGACTGGCTCTGTCTCTGCAGAAGGGAGATGC
TGATGCCATGAGCCTGGATGGAGGCTATATCTACACTGCGGGCAAGTGCGGTTTAGTTCCAGTCTTGGCA
GAGAACCAGAGTAAGTGGAGTTGGTGACCACAGGCTATGCCATATGAAGTTGACTTGGGAGTAGAGAGGT
GTTACCTCACTCACTAGATCTTGGAGAAGATGGAGACAGAATCAGAAGAAGGAATAAATGAAAGGGGCCA
TGACACCCACTTCACCCCATTCCCTTAGATTGACAGATCATGATAGTTTACTTCTTGATTAAAACAAAAA
CAAAGACATCTTTGGGAATGGTGAGATGGCTCAGCTGGTACAGGTGACAACGGCTGCCAAGCCTGTTGAC
CTAAGTTTGATGCCTAAGACCCACATGGTGGAAGGAACCAACTCCCACAAACTGTATGCAGCCCTCTATG
CATGCACACACAGGTCATGTGCACCTGACATTATGTATATATAACATATTATTATAAATATGTATTTATT
AACATATATGTTGCACACACAGAGCATGCATGTGCTGACAACAGGTGCATATTGACAACATGCATGTGCT
ACTAGCATGAATACACTGATAGCATAAATGTACTGGCACCCAGATGAAGGGTCAGCCATCCCCAGAAGTC
ACCCTGTGCCCTGCTGTCACCATACTTCCAAAAGGCAGCGACTCTCCTGACTTCCAAAGACATAGATCAC
CTTGCCTCTCCTTTAAGTTAATGGAAAAAGAATTGAGCATTTGATGTCTGCTGTGCCTTGCTCAGCATTA
TTTTTGTGACATCCACCCACACAAACGTGTGGCTGCTGATTAGCCAGTCTCGTGGCTGCATAACGTTCTA
TTGTGTGACTGGGTGGCCGTGCTTCTCCATTGTCTTCATGGATGGGTGTTTCAGTGATTTCCATCTGGTG
GTTATTGTGAAAGATACGGCTACAGCCATCCACGACCACGTCTATGATGAAAATGTGTATTTATTTCTGC
TGCATTGATATACCGAGGCGTTCCCTTTGAGTTTGATCTTATCGTATTGTGGGCATTTGAAAGTTTATGG
CCTCTCAGGAAATCTGAGTTGTCAGTAACGCTTTGCTTGTCTTTGCCCCTTCAGAATCCTCCAAAAGCAA
TGGCTTGGATTGTGTGAACAGACCAGTGGAAGGTAAGTCGGGGGCAAAAATAAGTCACCACTCGAGAGGA
GGCAGGGGGAAGGTCACTAACCACACAGAAGCCAAGCCTGCTGTTATTACAGATACAAATGGTTGATGGA
GGAATAATTACAACTAAAGTCTGAATGCATCCTCATGCTTGCTGGGGAACTTTCATGTGGTCACTCTTAA
TGCAAGCTTCTGTGGAAAGAGAAATGTTGCTCCCCCATTCTGTACATTCGAGGGAACAGTGGCCCAGAGA
GTCCCAGTGTCTCCGATCTCACTCATACTGGCACTACGCTGCTGCAGATGCTGGATTGGAACCAGGGCTT
CTCGCCTCCGCAAGCCCACGGCTCTGCACTAGCCTCAGCTGCTCTCCCGAGTTGGAGCTTGCTGAGCTGT
GGAAGCCTTGGTACTTGCCCTCAGTTGGGTTATCAGATGCCTTCGGAGGGGTTCAGCAACCATGTTAACT
CCCTCACTGTGCTGGGCTGGTTGCACTCAGCTCTTGGCGGTCCTTGGGTGCATGCCCTTAGCATTCATGA
AGCAGATCTACTGGCCACCACAGCACTTACCTGTGTGTGTGATTTGTGCATTGCTGAGACCAATCTCATT
GTTCAGAGTCTGTTGCCACTGGGTAAAGGTTTATAACTCAGCAATACTATGAGGCTGGTTGAGGACAGTT
GCCTGTAACCACTTAGAGGACCAATTTTTCATATTATTTTATTTAACAATACAGGTTGGTCTTCCCTTGA
CACAGAACACCTTTTTAAAATTTTCTTTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC
CTTCCTTCCTTCCTTCCTTTCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCT
TCCTTCCTTCCTTCCTTCCTTCTTTCCTTCCTTTTCCTAACAATGGCCAATGATCCATGGTCATGTAATC
CTGTTGTTTTTCCGGCCTGGGTTGGAGACACTGTACTTGAATCCATGTGCACCCTAGAAGCTAAGATGTT
CTCTCTGTCATCCGTTGCACAAGGGTACCTTGCTGTAGCAGCAGTTAGAAGAGAAGATGCTGGCTTCACC
TGGAGCTCTTTGAGAGGCAAGAAGTCCTGCCACACTGCCGTGGACAGGACCGCAGGCTGGAACATCCCCA
TGGGCCTGCTTGCTAACCAGACCAGATCCTGCAAATTTAGTAAGCATATGGGAGGTTCTCACGGGGTGGT
CATCCTGGGTCTGGGAGTGAGTACTTGAGGCTGTGGAACCTTAGGGGAGGGAGAGGGCAAGACGGTTGCT
GACTGGCTGTCGCTTGTGGGCCTCTTTTAGAATAATTAGGTTCTCCTTCTGGTCGCCTCATGGAACCATG
GGTGCACAGGATGTTTCACACAGAACACTTCCTAAGCTTCCCCAATTCCACCCCACCCACTGGGAGATCC
AGATCGCAATAGCATTTAATTAAAACATTAAGATATGCTCAACCTTATGAGTTAGCAGTTAGCTGAATTG
TTAACAATCAGATGTTTAGGTTTTTTGTTTTGTTTTGTTTTGGTTTGGTTTGTTTTTTTCTCATCGTAAA
TCTAGTAAGATAGCGTGAATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTATATG
TAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGAGGGAGTCAGATCTCATCAGATCTCATTACAGAT
GGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACCTTCGGAAGAGCAGTCAGGTGCTCTTA
CCCACTGAGCCATCTCACCAGCCCTAGCTTGAATTTCTAATGTAGGCAAGAATGCAGTAAAATGGGCATT
TTCAAATGTTTTCATGTGTATAAAAAACTGATACCTGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACT
GAACTGCTCTTCCAGAGGTTCTGAGTTCAATTCCCAGCAACCACATGGTGGTGGCTCATGACTATCTGAT
GGAATCTAATATCCTCTTTGGTATGTCTGAAGAGAGTGACAGTGTACTCACATACATAAAATAAATCTTT
TTTAAAAAAAAAAAAACAAACCCTGAGGCCTTTTAAGAATGTTTCCAGAGAGTTGGCACCCAGCACTCCT
GAACACAAATCATTAGACCTACCTAGTACCATGGGTGTACCAGTCACAGGAACATAACAGCCATCAGGCA
CCTTAATTGAGAAGCCTGGGTTTCTATTTCTGATTTTAAAAAATAAAAAGAAGTCAAGTTGTGGAGCAAG
AGGCCTATCTGTGCAGGTGTGGCCTTCTCAGACACCAGCCCTGATATCCTATCCATTTGTACAGTCTGAA
GCCTTTCTGATTGCCAGTGGGTCTTTGGTTGGTGGTAGAATGTCATTCTGGGGTGACAGGTATCCTGACT
GTGCTGTCTACGTACTGAGCTGACATCATGCTATGCCATCTGCTGGGTAAAGACTTTGTGTCGACTGTGG
TCCGAAGCACTAACTTATTGTCTTTCCTCTGTCTAGATGAGTTCTTTAGCCAAAGCTGTGCCCCTGGTGC
TGACCCCAAATCCAATCTCTGTGCCCTGTGTATTGGTGATGAGAAGGGTGAGAACAAGTGTGCTCCCAAC
AGCAAAGAGAGATACCAAGGCTACACTGGGGCTTTAAGGTAAGTCTGGGGCAGAGTGCCACCAGCCCAGG
GCCTCAGCCCCCATTCTCAACAAGCGGCACTGTAGTTGGGGTGGGGTGCTCTCATAGAAGAGTCAAGGAT
CAATGGGGGCAGAAGAAGCAAGAGCCTCCTATCATACCCAGCACCCCAGAGAGTAGAGGTTGGACAGCGG
GTCATGGGGCTTACAGGGCTGCAAGGCTCAGCTTTGTCACCCTCAAAGTCAACGGGTAGAGAGTGTTTCA
GTGCATTTCAGAGGATTCTAGTGACCACCACCCTTCCTTCTCCCTCACTCAGATCACCATTATCCTATGT
TTTTAGTTTTCTCCTTGATTTTGGCCTTGAAGGGGCTGGAGAGATGATTCAGCCGCTGAGTGCTTGCTGT
TTCATACAGAACTGCGTTCAGTTTCCAGGATCCCAATCAGGTCCAGGGGATTCAGTGTCCTCCTCTTAGG
TCCAGAGTACCCACATGTGCACATATGTACATACAGGTGCACACATAAATAATAATAGGTCTTTTTAAAA
AGAAGTGGCAAAGAGAAAGCTACATTACCACACCTTCTCAGTGCTGCATGGGTCACAATGACTGTACAGC
TCACATTTACGTGGGTGTTGATTCTCACGGCCCTGTGTAGAACATGAGGCTCACGCAGAGGCTCCTTCCT
CCATCCTTCCTTTAACACTAGATGTTGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT
TCCAAAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTAATGAGATCTGATG
CCCTCTTCTGGGGTGTCTGAAGACAGCTACAGTGTACTTACATATAATAAATAAACAAGTTAAAAAAAAA
CACTAGATGTTGTCACAGACCCTCAGATGACAATATTCAAACGGGACTTTGGGCTTTTAAATATAAATTC
AATAATTTTGCTCCCGTGAAACAATGACTTTTTTTTGGCTATATCTAAATGATTTGTAAACACTGATTTT
TTTACGTGTATGAAACATGTCCTTAAGGACTCACATAGCCGGGCATTGGTGGCGCACGCCTTTAATCCCA
GCACTAGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAAAGTGAGTTTCAGG
ACAGCCAGGGCTACACAGAGAAACCCTGTCTCAAAACAAACAAACAAACAAACAAACAAACAAACAAAAA
GATTTACATATAGAAAGCCTACAGCATGATAGCATGATTCACTCTTATTGATTGATTGATTCACTCTTTA
AATAGATAAGTTTGATAAGAAGAACCCAAGGGAATCCTGGGATCTGTTCCCCCAGACTTGCAGCCTGGTA
TTTTCTCTCTGGAAACATCTGCACACCCTTACTCCTGTATACACTAAAAGTTTTTCTTCTGCTCAATTAG
ATCTAGAATCTAGGCTGGGGACTATACAACAAAGTAATCCAAAGCTGGAGCCAGGACTCTTGCTTTTCTC
TTACATCTTTATCTAAAACTCATTTAGGTTAGCATATAGCATGCTGGGTTTCATCATGGTGTCTCTAAAT
TCACGTGACATTATGTTTGGTTCCCATCTGCTCCCCTGTACTCTGTCACTTGCTTCTTTCCTCCCCAGCT
CATCATTATTACATGCGTTCTTTACTCTCTCTAGTTCCCTGATTCCTTAAGATCTCTCCCTTCCAGGGAT
GGAGAGATGGCTCAGCAGTTAAGAGCCCTGGCTTCTCTTGCAGAGGACCCAGGCTTGGTTCTCAGCATCT
ACATGGTGATGCAAAAGCATTCCTCTGGCCTCAGCAGGCACCAGGCATTCAGCACACAAACATTAAGGAA
ACACTCATACACATAAAATAAAAATAAATAAATACTTTCAAAATGTTCTCCTCCTTCTCTCTCATAGTCC
TCGTTCTGGTTTCCTCACACACATGCATATACACACATACACACATATATACACATATACACATATACAC
ACACATATACACACATATACACATACATACACAACACACACATACACTCACAACACACACATACACTCAC
ATATACACATACATACACAAATGTATATATACACACACATACACACATATACACACATGCACACACATAC
ACACACAAACAAACATATACTTTTACATTATGGCCCCACATGTGAAAGAAAACATGTACTATTTGTCTGA
GTTTGGCTTATGAACATAATAATCTCCAGCTTCACACACTTTCTATAAACATAATGATTTCCTTTTTCTT
TAAACTCCCATTGTGAATTCGTAGCACATTTTCTCTACCCATTCATCTGTTGATGGACATCGTGGCTGCT
TCCACTTCTTGACTATGATGAATAGAGCTTCTATAGACACGGATATGCAAATATCTCTGTGGTAGTACAC
AGAGTTCTTTGGGCCTATACCCAGGTGTGGTATAGCTGGGCCGGATGATAGTTTCAGTCTCAGGTTCTTA
AGAAACGTCCACAGAGGCTGCACACGTTTCATTCTTGTATTAATAACTAGCATGCATATACTATTGGTTC
TTACCTTTTTTAAGTTTCACGTATTTTTCTGAGTTCCTGTTATGCTACAAAACCTAGCCTCTCACACTGT
CCCTGTCCCTCAATAACTCAACCACAAACTTGGCTTTTTCTGACACCTGGATTCATCTTATCGATGCTTG
TGTTGCAATGACTACTCCAGCATCATTCTCATACTGAGGATGTGGCACAAGAGCGTGTGTCCCTAGCCAG
GCATGGAGGTGCAGAGAAGTAGAACTTACTTCCTCAGGCTCTGAGGCAAGAGCTTCTACACGCTGAGCCA
TCTCCTCAGCCCTGTTTTGATCCCTAGTGTTCTGAGGCTTCATTTCTCTATGTGAGTCCTCTCTGCTTTA
TTAGAGTGGGCAGTTAGCAGACATGGATGGCTACAAATTATTCAGTTTGGTGAAATTATTATTTTGTTGA
TACTTTTTATGCTCTTGTGTTTTAAACTCCAGACTCTTGTGGGCCGAGATCTGAGTTCCTAATATCCATT
AGTCATATTTTCACTTCTAGTTTCTGTAAAATTTGCTTGGTTGAATCTTTTAATCCTGTGTCATTTTAAA
GCATTTTTATGGTGAAGATTATTTTTATTTTCCTTTTAAAAATATATATATATTTATTTATTTATTTATT
TTAAACATGTTTGGCTTGCATGTGTGTCTGCATACTACCTACATGCCTGGTGCCCTCAGAGTCAAGAAGA
GGGAGTCAGAGCCTCTGGAACTGGAGGGATGGATTGTTGAGAGCCTCCATGTGGGTGCTGGGAATAGAAC
CTAGGTCCTCTGGAAGAGCAGCCAGTGCTCTTAACCATTGATCCATCTCTCCAGCACCTATTTATTTTCT
TTTGAATCTTCCTAACAATTCTTTGTATTTCCATTATTCCTTTTTTTAAAATCTTCTTTTATGTGCTTCA
TGAATGTTTCTCATTTCTTTCTCATCTTTAATGATATTAAGTGTAATCCTCTCCCTTCTATCGTCTCTGA
ATCTTGGGAGTACAATGCTTTTGGTATCCCTTTTCATGCTCTCCCTTCCGGCATCTGACAGCCTTTGCCC
GTCTGACAGTGAGGTGGGTGAGTGCCACAGCTGACTCTCAGCTCTCCATCAGTTTTCCAGTGTGAGGTGC
TGGGCCCCAGTTCAGGGCTCTTGGTTTGTGCCATTTGTCATGAGCATCCTAATAGCAGAGTCTACGGATG
TTTTCTTGGACTCATCAGACTCTCTTGACGAAGAGTCTCACTGCCCACATCTTGGGAGGGATAGCCCTAA
TGGCCAGCCTGGGTGGTGCGTAGGGATTGACTCCATTTTCTAGAACACAGACTTTTCAGTTTACTCCTTT
ATGGATGGGGGTTGGAGGCGGGGAATGTTGAGACAGATCTAACCCTGTAGCCCAGGCTTGCTTCACACTC
ACAGCAATCCACTTGTCTCAGCTTCCAGAGTCCTGGGATGACTGGCATGAGTCGCTGTACCCAGCTCCCT
CTCCGTCTTCTCGGTTGTGTTTTCCTATACTTCCATTGGGTTTGTTTCCCTATACTGTGTGTGTTAAGGG
TCAACCTCCCAGATGTTGGGATGAGAGTTGCTGCATGGGATGGGAGCATTAAACTCTGTTTCCAGCCACT
CTCAGCGATGCCTTATATTTCCTGTTGCTGAGCCTTTCTGGCATCCCATGGTTGCACATTAACTTTCGTC
AGAGCTGCTACCACCCCTGCCTTATGTTACAGCCTTCCTCCTTCAGTTAGGCCAGGACTGCTCAGCCATT
TTCTTTCCAGATTCCAAAGGGTGTCTTCATTACCTTCTCTTTCTCTTTTATATGTTTATGGGTTAAAAAA
AAGAGAAATCTCATGGTGTCATCTCACTGGGGTTTTAGAGGGAGAGGTTACCCAGATGCTTGCAATCTGC
AGTGTTAAATCTTAAATCCCTGGAAACAGTTTCAACTTGTGTATATTTACTTTTATGTTTTAGTTTTTGG
AATGCATGTCACAAATATTTATGAGAATTCTAATGGTGTCTCCCTTGTGGTGTATTTATTTTGGAAAATA
TTTTAGGTGTCTGGCTGAGAAGGCAGGAAATGTTGCATTTTTGAAGGACTCCACTGTCTTGCAGAATACT
GACGGTATGTGAAGATGTTTCGCTTCCTCCTTCAGGAGCAGTCTTTAGCACCCTTAACATATCCTCATTT
TGGGGTTGGTCTTTGGAAAGATAGTGTAACTATTTCAGACTTTTGACTAAAAGAATTCTCAAATATTTTA
AATCTATCTTCCTGCCCCCCCTCTTTCTCTTTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTTTCTCTCT
CAAGGCAGAGTCTCATTTTATAACTCAACCTTGAACTTACTACATACCTCACATGGTCCTTGAGCTCATG
GAAGTCTTCCTCTCTTGGCAAACTGAGTTTGGGAAATTACAGGCATGAACCACACCAATTGGTGAGCTCT
CAAGTCTCTACTGCTGTAGGCAGCATAGAAACACCTGTAGTGTCACAGTGTTTTTGAAGCAATGCACACA
CACAGGTACACACATACAGATATACACACATTCACATAGACATACACACTTACATGCATATATACACAAA
CACACATATATGCATATATACATACACACATTCACACAGATGTACATACACATATACATAAGCATACATA
CTACACACACTGAGCTGCTACACATGAGTATATACATGAATATGTATGTACATACATACATCCACATATA
CATTCACACAGGCATACACACACACACACACACACACACACACACACACACATACACACCTGTCATAGTT
GGTAGCAGGCACATGAATGAAGAAACTCACGGTCTGTCTGTACCAGATGAAACAAGAACAGACAGTAATA
ATAACTGAACCCATTCAAATAAGATTTTATCTTGAAGATGGGAATAGAGGCATTTCTTCTTCTCATCTCC
ATATTAAAACCTTGGTCTAGAAACTCCTTGAGAGTCATTGAGTAAGAGTCCACACGAACATGTAGGTTAT
GAAGAGAATGTAGCAGCCAACATTAGAATAAGATCCCCCCCTCTTCTGGTCGCTAGATTGGGAAAACAAA
ATAGGGTACCCAACTCTTGCCAGCTGTTTGCTACTGCTCAAGTTGCTCTTCCTTCCAGGTCTCCTCAGCC
TCCATGCCTCTGCCTCTGAAGGCAACCTGCTGCTTTGTTCAGTAAACTCCAGTCCAGCCCCTTCTTCCTT
TCCCTTCTGCTTGCCTGTTGTCTCTGTGTGTGTGTGTTTGTGTGTGTGTGCTTGTGTGTGTGTGTGTGTG
TGTATGTGTGTGTGTTTGTGTGTGTGTGTATGTGTGTGTGTGTTTGTGTGTGTGAGGAGACCTCTGAGAC
CGAAATGCTCAGTATTAAGTAGAGAAAACCCAGGAAGGGGATGAAGTGTTTCCCAAGGAAGGAGAGTCAA
GCAGGAGCTTGGCTAATCAGCCTTTGTGTGCACATGGTGTGCCACATTTACACCCAGAACTCATGGCAGC
CTGTGCGTGATACTCCCTGAGTATCATCAGACTGATGTCAAATTTAACTTTAAGGCTCTTAGGTACTTAC
TACCTATGTGTATTGGAGTTAACTAGACCTATTGATAAGCCCATGGAGTTGCGCATTTTTTCCATGTTGT
ACTGAAATGAGACGGAAAAGACTGTCTGTGAAAGTCTGAGCTTGGAAATGCTCTAGGATTGGAAAGTTTT
CCCACACCACACGATATCTAACATGTGGGGGAATCCTGCACACTTGACCTCATCTGATGAGTCAGAGCCA
GACTGTGGTCACTCTGGCAACACTAGGCAATATGACCTTCAGGTGACACCTGCAATTTGCATATGAAACA
TAATGGAATATTTTGTTTATTTTTGCATTACCATGACCACAGTATTTCATTATGTACATACAAATGTTCA
AAAACTTGAACAAATTTCAAAGCAAAAGTAGTTCTGGTTCCAAACGTTTTGAATAAAGGGAACTCAACCT
GCTTTCTTTCTTTGGAAACTACTAGAAAATATATTGCATAAATGGAACTCTTTTGTAAAAGGAAGGGGCA
GGAAGATACATCAGTGGGTAAAGTGCATGTTATGCAAGCATTAAGACTTGAGTTCGCACTTGGGAGGCAG
AGACAGGCAAATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGCAGACAGAATGTCAT
GGGCCTGCAGTCCCAGCTCTGAGGATGACAGGAATGGAGATAGGTGGATCCCCAGCCTTCTGGTCTAGCT
GAATCAGTGAGCTCTGGGTTCTATGGAAAACTGTGTCTCAAAAAAAGAGCAAAGAAGCGATTGAGAAAGA
TACCCATACGTAACTGCACAGGCAAGCATACCTGAACATATGTGTATGCACACATACAACACACACACAC
ACACTTAAAAGAACATTTTTAAAAAGATTTACTTATTTACTCTGCATATAGTTTTCTGCTTGTAGGCCTG
AAGAGGGCATCAGATCCCATTATAGATGGTTGTGAGCCACCATATGGTTGCTGGGAATTGAACTCAGGAC
CTCTGGAAGAGCAGTCAGAGCTCTTAACCTCTGAGCTATCTCTTCAGCCCTTAAAAGAACATTTAACAGC
TTGATTGGGATACAACTTGGTGATTTCAGAATCCACCCATTGTCTGTGTACAATCGAGTGGGCTCTAACA
CACTCAGGATGGTGAAACCACCAATGCTACCATCCAACTCCACAACACTCATTTGAAAAACATGAAGCAC
CCCATCTGTTGGCAGCCCACTGTCTTTGCTTTTTCAACATCTGCTTCCTGTTCCCATGGCTTTGTCTGTT
CTCATAAATGCAACTATCATTTGTGGCCCTTTGTAGCTGGCTTCTTTATCATAGCAAAATTGTGAGAATC
AGCCATGTTACCCCATGAATGAACTTCTTATTAAATAAGAATTTCCTTTATGACTAAGCTCGACATATTT
GTCGCCTGAATAGCTTGTGAAGGATAAAGTTGCATTTTGTCTCCTGGACATCACCACAGCTTTCAAACTA
GTCATACCTTGACCTAGCAGACTAGTTATGGCTAATGGTACCTGTCTTTTACCAGGGAAGAACACTGAAG
AGTGGGCTAGGAACTTAAAGCTGAAGGACTTTGAGCTTTTGTGCCTTGATGACACCCGGAAACCTGTGAC
TGAGGCTAAGAACTGCCACCTAGCCATAGCCCCAAACCATGCTGTAGTGTCTCGGACAGACAAGGTGGAA
GTCCTTCAGCAGGTGCTGCTTGACCAACAGGTATGGACCAGCAGGGCTCCCAGCATGCTCTTCACTGCAC
TGGCTCCTGCCGGGAGGGGGTTCATCCACTCAGACCTTTCTTTCTAGCCTTTCTGCTTGATCGATGGGGA
TAGCGTTTGCTGCATGCTGTGAGTCTTTCTAGACTTCTGCCTGGATTCTCGGCTCCCAAACTTGAACTTT
CTGACCTCTCCCTCCTGCTGCTTCCTCTAGTGACTCAGAGAGAGGGACGAACAGTGGCCAGGACAGAGGC
TGTGAAGCAGTAAATCCAGAGTGTGTTCCATGTGCAGGAGACACGAGGTCTTTGTGCTCACAGATTAAAT
ACTAGGAAAAGCAGGAATATTGAACATGTGGCGTTGTCTCTTCAAAAAAGAGATGTATGACCTATTTTTG
CCAAGAAGCCTGGGAACAGGCGAGGTTAACAGCCTGGCGACTTCTACACTATCTGTATGGCCCAGCCATT
CTGGATCCCAGACTATATGCCTGCCTCAGTCAGTCTATAACATTATCTTCTCTGAGCCTCTTATCCCTGA
GCCTGGTTTTATAGAACCCATCTTTATGGAAGATGCCTTGTTCCTTACAAGAGTTATTCATGTAATCATG
TCTTAAGCTTTGTTGTATACACAGGACAGTTTTTTCCCCCCTTAAATTGTACTGTGCTTAAATATGCCTG
AAACAGACTACCCAGTGTCAGGGTTTTGAAGTTTGACCTAACACTGGCTACTGAGTTGTGTTGAACCAAA
CTTCCTTCTTGCCTCAAACACAGTGCTGGCCCTGGTGTTTGTACGGAGCCCCGCGTCTGTGGATTTAGGG
ATGACTGGGTTCTAGTGTTCCCTGTTCTAGTTTCTATTTTCCTATCACAGCATGTTGGCTCTGGGTACAC
TTTTGAGGCCAACAGCCCTGAGTAAGGAGAAGGGACTGCAGCCGAGAGAGGCTCAATGCCCTGTGGTGGC
CCAGTTCACAGAAACATGGGAATTTTTAAATTACCCGTTTGGACAGCTGGACACTACAATGTTCTAGATG
ATCAGCTTTTGAGGGAAAATCTCAAAACATCTTCGTCTGAACTCTAATCACCAGCAGGAAGTGATAACAG
ATTTAGTAAAAGGAAAGATATGGGAGGCAAGGTCAGAAGTGGTGTGAGGCTCCAGCCCTGGCTGAAGTGG
GCATGCTGCCAGGGGGAGCACAGCACTGGGAGCACGGTGCTGGGAAGAGTCATCCCAGGCAGAGAAAAGA
ACACTGCTGCTTTTTAGCTCTGCTGTTTACCCAAAATGGAGGAACCCTTATCATTCCCGCCCCCCACTCT
CTGACTACTCACAGAACAGTCCACTCTCTTGACACAGGTTCAGTTTGGGAGAAATGGACAGAGGTGTCCA
GGAGAGTTTTGCCTGTTCCAGTCTAAAACCAAAAACCTTCTGTTCAATGACAACACTGAGTGTCTGGCCA
AGATCCCCGGCAAAACCACATCGGAGAAGTATCTGGGAAAGGAGTACGTCATAGCGACCGAGCGCCTGAA
GCAGTGCTCCAGCTCCCGTGAGTGGGTTCTAGATATAATTGCCAGGGACCAACATGGGAGAAGCTCCGGG
TTCGGAGGCCAAAATGCATGGAGAAGTGCAAGTTCTTAAAACCTTACAATGTGGAGCCAAAGAGATGGCT
CAGGGGGTAAGAGACGGACTGCTCTTCCAGATGACACCGGTTCAGTCCAGAGCAACTGTCCATCTGGCAG
CTCGCAGCTGTCTGTAACTCCAGTTCCAGGGAAGCTGACACTTCTTCTGGCCTCTGTAGGCACCAGGCCG
GCATGTAGTCATCAGACATCCGTGCAGGCAAAGCGTTCACACACATGACACAATAAGTTCAAATGTAGGA
AAGTATAGTGATAATAATTCCATTCTCGAAAACTGTTGGGAAGAGTGGCTGAAGAGTTAGTAGATTATTT
GCCTCCCGTGCACAAAGCCCTGGGTCCAGCCTCTAGCATGGCATAAACTGTGTGGTGCACACCTGTAACC
CCAGAACTCGAGAGGTAGAAACAAGACAACCAGATGTTCTAAGTCATCCTTGGCTACATATGGAGATCGA
AACCAGCCTGGGGGACATAAAACCCTATCTCAAAAAAATTTCAAATACCTGTTATACATATATACATGCA
TACACACACACACACATACACACACACATACACACACACACATATACACACACACATACACACACACATA
TACACACACACATATACGCGCACACACACACATATACACACACATATATACACACACATATATACACACA
CACACATACATACAGATTGCAATCCCCAAGCATCCCCACGCACCGATAGCCTGTGCACAGTAGCCCCTTG
AATCCCTACATCTGGTGGTCCCTACTCTGAGAGGCAGAGGCATGGCAGATGTTGTGGTCCAGGAGTCGGA
GCAGGCCCAGAATCTCCTTGTCAGTTGTTCTCAACTTAGGACAATTTTATCCCCAAAGCAGACATTTGGC
CATGCCTAGAAACATCTTTGATGACCACAGTTTAGTAGGTGCTTCTGGTAGGAGGCAGTAGAGGTTGGGG
TCATTAGCAAGCAATGCATAGGATACCACAGCCTACGAAAAAGGATTGTCAGGTTCCCTTTGCTGGTGGG
CCTGAGGTGAGGAGCCACGATCTGTATTGAGCAACGTCTCAGGCTGTGGCAGGTGTGGTCCACCCTGGGT
GACCCAGGGCTATTGATGAACCTGTGAACAGAAGGCACTGGTGCAGAGGCCATGTGCTCGAGGACAGAGC
TGTCTGCCCATGTTCTCCCAGCTCATGGTGAAGTGGCTCACACAAGGCAGGTGCTCAGCCAGTATTTGCA
GATGAAGGATAAGCAAACAGATCAGCCTTCATCGTGCACCATTCAGGAGCCAGAGGACCAGTGTCTTGTG
AACTTGAGTGGTGTGAACTGATTCACTTAGAGGTGGAGTCACATGACCCTCAAGTCAGAGCAGGGAGTTG
CTGTGGCCCAGGGCGCCACAGTTGCTAGAAGAGGTGGTAGAAAACATGTGGACGGACGAGGCAAGCCCTG
ACTCACGATGGCAGGACAAGGGCTTGGACACTGTGTTTGAAACCTCTTTGATGTATAGATTTGGGGGGAA
ATCTGTGTCTGAATGACCTGCTTTCTGTGTCCCACAGCACTCCTGGAAGCCTGCGCTTTTCTTACCCAGT
GAAAACACTGAGCAAATAGCAGAACCTTCCCAGGAAGTCTCATCCCGGAGCCACGGTCCGGGGGCCTTCA
GACCATCTGGTCTCCTCACTCCCTGCTGTCACTTTAGGTAGAAATAAAATGAAGTAGTGTTGAGTTTCTC
GTCATATGAGGTGTCACTCATTGCTCCTCAAAGTTTATACTGGAATCCTGTGTCTGACCACTTGCCTGCA
CACTTGGACTTGCTTTTGGAGATTCTCGGTCCAGCCCTTCCTTTATCCTGAAGTTTCAGGCATGGTGCCC
ATTGACCTGGAGTCTGGGGACAACCACGTGATGTTTCTGTCCCCAGGGGATCTGGTTTCTTCACATAGAG
ATCACAGTACATGTGCATCTGTGTGCCCCAGATCATGCGTGCGTATGGGTGACCATGTATGCCTCAACAT
AAGTTTGCACATGACTGTGTGCACTCCTTGCACTGATGTTTGCATAGGACTGTGTACTTGCACTAATGCT
TATGTGTGTGTGTGCACACACGTGTGAAAGCCAGAGGACAATCTGGAATGTCACCATCCACCTTTTTTAA
AAATTAAATTTATTTATTTACATCC

Protein Sequence : Show Sequence

>gi|31560677|ref|NP_032548.2| lactotransferrin precursor [Mus musculus]
MRLLIPSLIFLEALGLCLAKATTVQWCAVSNSEEEKCLRWQNEMRKVGGPPLSCVKKSSTRQCIQAIVTN
RADAMTLDGGTMFDAGKPPYKLRPVAAEVYGTKEQPRTHYYAVAVVKNSSNFHLNQLQGLRSCHTGIGRS
AGWKIPIGTLRPYLNWNGPPASLEEAVSKFFSKSCVPGAQKDRFPNLCSSCAGTGANKCASSPEEPYSGY
AGALRCLRDNAGDVAFTRGSTVFEELPNKAERDQYKLLCPDNTWKPVTEYKECHLAQVPSHAVVSRSTND
KEEAIWELLRQSQEKFGKKQASGFQLFASPSGQKDLLFKESAIGFVRVPQKVDVGLYLTFSYTTSIQNLN
KKQQDVIASKARVTWCAVGSEEKRKCDQWNRASRGRVTCISFPTTEDCIVAIMKGDADAMSLDGGYIYTA
GKCGLVPVLAENQKSSKSNGLDCVNRPVEGYLAVAAVRREDAGFTWSSLRGKKSCHTAVDRTAGWNIPMG
LLANQTRSCKFNEFFSQSCAPGADPKSNLCALCIGDEKGENKCAPNSKERYQGYTGALRCLAEKAGNVAF
LKDSTVLQNTDGKNTEEWARNLKLKDFELLCLDDTRKPVTEAKNCHLAIAPNHAVVSRTDKVEVLQQVLL
DQQVQFGRNGQRCPGEFCLFQSKTKNLLFNDNTECLAKIPGKTTSEKYLGKEYVIATERLKQCSSSPLLE
ACAFLTQ

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

33. Nos2

Gene Name : Nos2
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 18126
NCBI Protein GI : 6754872
Locus Tag : RP23-341J22.2
Genbank Accession : AF427516
Protein Accession : NP_035057
Taxonomy ID : 10090
Gene Starting Position : 78920858
Gene Ending Position : 78960123
Gene Strand (Orientation) : +
Protein Name : nitric oxide synthase 2, inducible
Protein pI : 7.76
Protein Weight : 123528.57
Protein Length : 1144
Protein Note : Also known as iNOS; Nos-2; Nos2a; i-NOS; NOS-II

DNA Sequence : Show Sequence

>gi|372099099:78920858-78960123 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J
TGGCCCCACGGGACACAGTGTCACTGGTTTGAAACTTCTCAGCCACCTTGGTGAAGGGACTGAGCTGTTA
GAGACACTTCTGAGGCTCCTCACGCTTGGGTCTTGTTCACTCCACGGAGTAGCCTAGTCAACTGCAAGGT
GAGTCTTGTGTTGGGGACAGCTGCTCTTAGGAGTGTCTTCCTGCTTGGGAAAGCCCAGAAAACCAGGCTT
TCCCTTCTCCCCCCACCCCACCCCTCTTTTTGTGGCTGGACTGAAGCTGATATGGAAGGTGTAAGAAGCA
CACCCACAGAAGGGTCTGCCAGTTGAACAGGGCGTGTGGTCAGATTTCTTCTCTGCAAATGTTTACTGTG
GTGATTACACTACAAAGGATAAACTTTCTAGAAAGCTCCGAATAGGCTGGGGGGGGTGGTGTTAATACAT
ATAGCTTACTTTACTCATCTTTATGAGATGTGCCCTCTGCCCCACCCAGATCTGATAAGATGGTTATTAT
TAATCCCATTTTAGGAGTGAAGCAATGAGTTTCAGTTAACTAACTTGATTTAGATGAGTAACTAGAAAAA
CTCAGGGTAGGGTCCAGTTCATCGGGACTCTGAGTTTCACTGTTCCCCTTAGGTGGGTTCTTGTACCCCC
CCCCCCCGTATCTTCCAAGTATATCTTTCCTCAGGGAGGGCACTCGCCAAGGAGAAGCCACATACTCTCA
GGGCAGTTCCACTTTCTATTTCTTGTCTTTTTCGTAAAGGATATAATCTCAACTCTGGAGAGTTTAATGC
TCTTTTATACAAATCATTGACTTCTTCTCAAGTAACAAAGAAGAAGAAGGAACATGTGACCTAGTGTCCT
TGGCTCAGGATGCGTGATGACTCCCAAATCAAAGGAAAGGTGGCCAGGACTTGAGTGCTAGCCCCAAGTG
CAGGTCCAGAGGGAAGGGTACTAATGACAAGTGCTTGCCCCTCTCTCTGGTCAGCAGGTGGCAGGAAGGG
TCAGGCAGATGCACACACTCCTGTATTAGTGACCCCAGCTCCTGCCCACCTTGCTGGAGGCCATGGAGTA
ACTCCTGCCAAGAGAAACTGAATTGTCCTAAGGAAGCTTGTGGATGCTTGCTGTCCTTGGTAGGTGGCTA
TGTGGCATCCCAAGTTATCCCAAGTTTGGTTTGTTATGTGTCTTTGTAGAACTGGAGTCTGTCCCACAAG
ATTTCAACTCCAAATAAAACTGCCGAGTGCAAGCATGGAGAGAGAGCACTTCTGTGCTAGCCTTAGCTGG
GCTCTGTCTCCATTCTCTGGGACACTAGCGCCCGCCCGTGAGAAGGCTCTGTTCTGTTAGGCTCTTCTTC
AACCTCACCTCACAGCCTCCCTCCCCTTCCTCAGAGAACGGAGAACGTTGGATTTGGAGCAGAAGTGCAA
AGTCTCAGACATGGCTTGCCCCTGGAAGTTTCTCTTCAAAGTCAAATCCTACCAAAGTGACCTGAAAGAG
GAAAAGGACATTAACAACAACGTGAAGAAAACCCCTTGTGCTGTTCTCAGGTAAGCAACCTCTCTCCCAC
TTCCAGCTTTCTCCTCAGGGCTTCCAGGCCCCTGCTGCCTCTCTCTGTTCTGGGCCACCTAAGAGAGTCT
GGTCTCCAAGGCCCTGCTTGTAATACTCTACTGGAGTCTGATGCACCTTCCTTGGTGTGGACAAGGAAGG
CCCAGGATCCCCATGCTGCTCCTGAGTGAGTCTGTACTCTCTGGAGGAAGAAGGAAGGGGGAAGGGGAAT
ATGAGAGAACGGAGACTGGAGAGCCTAGTGGTCAGCTCAGTAGTGACGTCCTGATTGGCCAGGGCATGTG
CCTCACCAGTTAGATATTTTGACTATTAGGCAACTGAAGGGTGTCAAGATGGCCCTGTACTATCCTCAGT
GACTGTGTGACTTTGAGTAAGTGCACCTGTCTGAGTCTCAGGTCTGCAGTGTGATTTACCTGATAGTCAC
AGAAGGGCTTGTCACCATTTAAGAGGCTTCTTTGTCACAGGACTACACCGTGCACACACCTACACTCTTT
GAACGTAACCTTCCTGGGGCCCTTAGTTATGATCTCATTTTGCAAGTGAGGCTCTGGAGGGCAAAGTCTG
GCTTACATGACTGCTAATGTCAGAGACGAAATCTGAGTTTGGCCTGAGGCATCTTAGAATTGAACTAGTC
CTCCAACAATTTATCCGGATGGACTAGGAGAATTCTAGAGAATGCTAGGAAACTACAGCCATAGCCAGGG
ACTTGAGGCTAACTTACGTAGAAAGATGATGAGCTGGCTTTCTTTGCAATGTGACTGCACAGAATCACGT
CCAAGTCTCAGTCTGTACCTGGCTCAACATCTGCAGGACTACAGTGGACAGCGCCCCCTGGAGGCAGTAA
GCAGGAAATCTCTAAATTTCCCTGTCCTGGTGTTGCCAGGTTTAGAAAGCAAGTTTAGGCAGAAGGGAGT
AGCTTTGCCACTTCATAATCCTGTGGCATTTGTGACGTCCCTTAGCCTCCAAACCTCTGCCTCCTCACTT
GCAGGAAAATAGTCATTATGTTAGGAGTAGAGGAGACAGATGGCTCAGGAGTGACAGGCAGAGTGAGTGG
GAAGGGACTGGACCACAGCTGTCAGCAATCCTGGGGCTCAGGTGCCCTCTAGCACTTCAGTCTCAAAATA
CCGTGACTTCATCCCGTTTTTTTCTCTTTACCCGGTCCCTACAACCCCTACCTTCTGTCGCCTCAGAATC
CAGGTGCCTCCACACCTCTTTCCCTCCAACTTGACTTCATAGAAGTCATGCCCCTGCTCCAAAATCTTGG
GTAGCTGCTGGTTACCTGTCATCACACTGAGACAAGAAGGAGGGCACATCCTCTGACAATCCAGAAGGGG
ACTGGGTTGTCCTCCCACTTTAGGGAAGAGGAGACAAAAGTCCACCGTGCTTGAGTCATCTGAAGATGGC
AGTGGTGAAGCAGGCATTCAAACTGGACAGTCTGGCTTATGGCCACCATCCTGGTAGCTACACTGTAGGT
CTCCAATCGAGATGTATCCCAAGGACAAGGACACTTGAGGCTTTCTCACCTGGCACTATACTGCCAGTTT
ACATGTCAACAGAGCCCTTCTGCTTCATCAGTGGATTGATGCAACCATTTATTAACTGCATATATGGCCA
CACATATTTATAGTGTCCTTAACACTATGTCAAAAACCAGGCAGGAACTGCTTTTATCGTGTCTAAAGAG
GTCAGTTTCTAGAAAGTGGAAGAGCTGAGATTTGAGCCTAACTCCTGTCTCTGAGGACTTAGTAAACTCT
GGGCTGAGAAAGAGACAGATCCAGTCGCTACAGTCAGAGAGGAAACGGGTGGGGTGGGGGAGGGGGTTGC
GCATGGAGAGCAACAGTTTCCTCCCTAGTTGGAGGCAGTCTTGGCAAGGCAAGATGTTAGAGAGGTTTAT
GCGTCTCTGAGCCTCCTCCTAAGGGCACCGCCTGGCTGTGGGGTCAGGAGGGCCTAATGAGCTGCAGGTG
GCTTTCTTAGAGGAGGGGGCCCAGCCGAGATGGGACTGCCCAGGAAAGGTGGCAAGAGGATGTTCCTGGA
AGAGGAAGCCGCGTACAAAGGGGATTTTAGTCTGAAAGCAGTGGGAGCCCGTTATTTATTTTTATTTTAA
GACATAATTTTCAAATCTATGCAGACGAAGAGCCTATTCCTGGTGGCTTTCAACTTTGTGTAAACAAGAG
ATTCAGAAGTGTGCAGGGTGTGTCCTTGCATGTGTTTTAGAGACAAAGGATAAATGTGGGACCGTCTAGG
TGACCCACAGGGAGTTTAGAGCAAAGGGTTTCAGGATGCTTAAAAGTACAAAACAAAACAAACAAAAACA
CCCAAAACACTCCTCGCACTGTAATCGGGCTTGGGGATTCATTTGCTGAAATATTAGCCAGAAGGGTCCC
TGAGGGCTGTAGCCCTATTTCACCTGCAACAGGGAGAAAGCGCAAAACATTTCCTGGGAGCGCTCTAGTG
AAGCAAAGGTAAGAGATGACCCTAGGGCTGTTGTATGGCCTTGAGCAGGATCCTCATTTCTCTGGTCCCC
CAGCTTCTGCCGCCATAAAGCAGGGGTAGTAAAGAATTGCCTTCTTAGTTGGATTTCGTTAGGGTGTGAG
GACATAACCCGGTGCAGCGGGGAGTCTGGGTCGCGATCCTTACTGTCCCTTCGAGAGGAATACACAAACG
AGGCACTCGCGCGCTCCTGCTTGTCCTGGGCCACTAAGAGCTGAGTCCTGCCCGCAGCAGGAGGGCGGGC
GCTACGGGGGCGGGCGCTGAGGGGTTTCGCGGCGATAAAGGGACAGCGCCAAAGAGGAGCGCTGGCCCAG
CTATGCGCGAGGGGCTGAGCAGCGCTTGCGACGCCGCCCGGCCGCGGCTCTGTCGCTTCCTGCGCAGGAT
CTTCGGCCCGAAGGTCGCCAGTCGTGTGGGCCCAGCCAGGTACAGAGCGCGGGACCTGGGGAGGGGCCCG
GGTGGCCCCGCTGGGACGCGAGAGAGGCGGGTCAGAGCGCCCACGCCGGCGGCCCTGTAAGGGTTTCTGC
ACCAAGGAACTTTTGCGCTGCACTGCTTTGGACAGGTTCTAGCTATAACGATTACTTGGCGTCAAACCTG
GAAATCTTCCTGCCTCGGCTTCTCAAATGCTGAGATTACAGGCGTGTGCCACCGCTTAAAGATGTTTTTT
TGTTGGGGGGGGGGGGGGAAGCAGTACTGGGAACTGAACTGCATTTAAGCAAGTGCTCTTCCACTGAGCC
ACTCTCCCCCTCCCGGCTTGCTGCTCTCCCTCCTTAATGCGCTCTGAACAAACAAGGTTAAGAAGCCAAA
GATGATCTTTTACGATAAGACGTTCTCCCTTTCACAAGAGAGGTGGGCTGGCTGGTAGCAGTTCGATCCC
AGGACTCAAAACCCTAAGTAAGCCCCTGCTGTGGGGTCTGCACTCACCCCTCAGTACCTAGTTCTGGGAC
ACCGTGACAGCAGATGGTTCTATGGACCTGAGCAAGCCACCACAGTTCCCTTTGTCCGAAAGTCCATAAA
GGTCGTTCTCCCTGCAATGGGCCAGTCAGGTCTCAGCAAGGCTGTGGGTTTAGAACTGCAGGAATTTGGA
GCTAAGGGTCCCGAGCCTTAAAGTGTTCACCTGATATCTGGTACCTAGATTCCCTTTAACAGGTGGCAGA
AGAAATCTGCTAGCAGGACATAATAGCTGGGGCAAAGCTTTATTTATCGTTGTGGGGTTTTCCCCTTAAT
CTCTATTATAGCAGTACTTGAAAAATTATAAAAGTTCCAATTATAATAAAAACGTCAAAATACTTAAAAG
TTAAAAAGAAAGAGATTTAAGTCACCCGTGGCTTATCACATCGTGCTAATCACTGTGAATCTTTGCTGGC
ATTTCCCCCAGTTGCTTTCAGGCCTGCTGGAGCCTGCATTCATTCACACACAAGCACACATTGCATGCGT
GCACAAAGACTGTCATCACAGCGTCACAGATATCCAGGCTGAGGCATTAGGCACAACCTCAGGCTGTGGA
CCCTGAGGCCTGCCTAACTGTGGCTCATGTCAGCAGTGAGACTGCAGACAGCATTTAAAACATTTTGCCG
TCTGGGGCAGGTTTACATCCTCACCAGAAGGAGGATCCCCAGAAAGCAAATCTTGGCTTCCGGATTTTTT
GGGTTTTTTGTTTTTGTTTTTGTTTTTGTTTTTTTTCCTCCTTCTGGTGAGGTATACCAGTCCAGATCAG
AGGAATATGTTAAAGAAAATATTATTCAGATGGGTGTGGTGGCACATGCCTGTAACCCCAACATTCAGAA
GGTAACATCAAGAGTTCAAGGACATTGTCAGGCAAATGGTGAGTTCAAAGGCAGTGGAAGGTCATGAAAC
ACTGTCACAAGCAAGAAGGAGAGAATTGTTCAATGAGTTTCTTGGTTTGCTTTGGTGGTGGCGGTGGGAA
GTTATTGTTTTGCTTTGTTGTTTGAGATAGCCCCTCACCTGAGATAGCCCAGGCTAAGCAAGTCTCCTGC
CTCACCTTCTGAATGCTGGGGTTTCAGACACCAGTCAGTGTATGTATCCCTCTTTAGTGATACCGGCTAA
CATACCGCAAAGAGGACTTTCCTGAGGACTGTCCCTCAGGTGAGTCCAGGACCCACTGCAGTCCGTAGCA
GAGGGAGAGATAGGGCTCCACCCAAACCCAGCAGGGGCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGG
GCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGGGCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGGGC
AAGGGCAAGTTACAGCCAAAGGTAAAGAAGAGCCTCACTGAACTGACCTAACAATCAAACCAGGGATCGA
GTTATTACCAAAACACAAAGCAGGGCCATAGCTAAGCTGGAGTTTGGGGGGTTTGTTTTGTTTTGTTTTA
GATTTATTTATGTGTATGGATGTTTTGTCTGACTGTATGGGTGTATAGCCATGGCTGCCCTGGAACTCTG
TAGACCAGGCTAGCCTCGAACATAGAGCTCAGCCTGCCTCTGCCTCTTAAGTGCTGAGATTAAAGGGACC
CACCACCATAGCTGGCTATATATATATATATATATATATACACACACACACACACATATATACATATATA
TATATATATGCACACACACATATATATACACACACACACATATATATACACATATATATGTATATATGTG
TGTGTTTCTTTTTATATTATATATATATAATTTTTATAAAATTAAATTTATTTTAATTTAATTTTTATCT
TATTTTCAAAACAGGTAGCATATAGCCCAGGCTAGCTTTGAATTCCCTACTTAGCTGAGGATGGATGACC
TTGAACTCCTGATCCCCCTGCCTCAACTTTCCAGGTGTTAGAATCATAAGTGTGTGCCTTCTGTACCTGC
CTTTGTTCCTTTCACTCTTTACAAGCACGGGGCTTGAACCTGGAATCTCTGCACTCAAGAAGCCCTGGCA
AGCGGATGGAGGCCAGTGCGGTGCACAGAGTAAACAAACGAAATCAAGGGTTTGGGGTGCTTTGTAGGTT
CTCTTGGAGCCCAAACTGGCTTTGAAGTTTCTTTGTAGTTGAGGTTGGCCTCAAACTCCTGACCTTTCTG
TCACAACCTGCCAGATGCTAGGATTCCAATTTGTAAAATGCCCAGCTTGAACTAAGAGATGAGCTGGGCC
TGGTGATGCATGCTTTTAATCCCAGCACTCAGGAGCCACAGGCAGGTGGATCTCTGTGAGTTCAGGGCCA
GTCTGGTCTACCTAGAAAGTCCCACATCTTAAACATACAATACTCACCAAAATGAAAACCGAAAATACAT
AAGATAAAGTTTGCTAAGCCAAGTAGGTGGTGAGTGATGGACCCTGCTGGCCTGGGAGGGCTTTTGCACT
TGGCCCTCTGAGGCAGGCATCTCCCCTGAACTTGTGTGTGTGCCTCTGTCTGAGTAGTTTTGGCTGGCTC
CCCTCATATCATTTTGGCTTTGAGTGGTCAAAGCAAAGATGAAAAGGGAAGTGGGGACAAGATTCTCTAA
GACTGGCTCCCTCATCCTTCCTGATTGGAAGCCGGGAACAGGTCATTCCCACCTCTAGCCCTTGGTACCC
CGTCTGCCAGATCGGGACTCACAGAGGCCACTGGAGATCCCCTCCGTGGCCAGACTCCATCTCACAATGA
CTGTTTCCTTTCGCAGCCCAACAATACAAGATGACCCTAAGAGTCACCAAAATGGCTCCCCGCAGCTCCT
CACTGGGACAGCACAGGTCAGTGGTTTGGTCATACAGTATGGGAAGCCCTTCATTCTTTTCCTTGGTAGA
TTCAGGCTAAACAGCCCTTTGGAAGCTGGGCAGGAAGGCACAGGAAGCCAAGGGCTGGGGAATAAGTGGA
GCACCCCAGACTTAGGGTACAGAGGGAGCCAAGGAGGTTGCAGACAGTAGGACAGTCTTGATGCTCATCA
ATAGAGGTCCCCAGGGTGCGCTGGTACCTAAGTAACTTGCCTGGTGGTTGACAGAATGGGTCGGGGTAGT
GCCATTAGGGCTCTGTTTCTGTCAAGAGATAGCATGACCAAGGTAATTCTTTTAAAGGACTACATTTAAG
TGGGGCTGGCTTACAGGTTCAGAGGTTCAGTCCATTATCATCAAGGTGGGAACATGGCAGCATCCAGGCA
GGCATGGTGCAGGAAGAGCTGAGAGTTCTACATCTTGTTCTGAAAGCAAACTGGAGAAAACTGTCTCTTC
CAGGCAGCTAGGAGAAGGGTCTCAAAGCCCACACCCAAAGTGACACACTTCCTCCAACAAGGCCACACCT
ATAAGCAGTATCATTCCCTATGGGCCAAGCATATTCAAACCACCACAGGTAGTTTAGGAGCATTCTCTAA
ATCAGGGAAGGACGGTTTTCCAGAATGATTTCAGATTCAGCTTCTGAGTCCCTAGTCTCAGCCCCTGGCT
TCTTCCATCTTCCTGAAGCCCCTTACATCTTAAGGACTGTAGAGGGGTGGCAGAGAGATGGAGCCGGGAA
AGGTGTCCCTGGGGAAGGGAGACAGTGGAGTATCTGTTGACTTCCCAGCTCTGCCCTTGGCAGTCCCAGC
CACAGCTTCGGCCCTCCCATCAGACTTGAATTCCCCTTATGAGCCAGCACTGGCCAGGCCTTGGTAAGGA
CAGAAGTCTGGCCTTGGTCCAGCCACCTGCAGTGCTCAGCTGGCACTAGCCCAACTTCTGGGAAGGGTGG
AGGACCGAGGACTGGCCCCTTGCCTTGTGTGCTCACGCAGAGCTCCGTCCCTCATCCAAGCAGAGTACAG
AGGCTCAGGGGACACTTGTATGGTTGTTGAGGTTATAGACATTTAGAAAACTCTTGATGTTTACCCATTT
TAGAATGTTCCAGAATCCCTGGACAAGCTGCATGTGACATCGACCCGTCCACAGTATGTGAGGATCAAAA
ACTGGGGCAGTGGAGAGATTTTGCATGACACTCTTCACCACAAGGCCACATCGGTAAGAAGTCTGGGGGC
TGGGGAACACCACCTTTGTCCCAACTAGGCCATCATAAGGGCCCTTTGTGGGTCTATCACATATTTCCTG
GTCTTATTAAACAGCCATCTTCTCAGACAAAAGATGTTGGGAAGTCTGTCTTTATAAATTATAGCAAGAA
GAAGGAGAGGTTAACTACTGGGTTAGACAGCAGATAACCTAAGATGTCAGACAAAAAGCTACAGAAACCA
GTAGAGTATCTGAGAATTGTCTATTAGAATTGCCCAAAGCTGGACTGAACTGGCAGAGGAAGCTTCCTGA
CTCTTTAAGGACAGCAAGGTAAGCCAGGCAGTGGTGGCAAATGCCTTTAACCAGCACTTGGGAGGCAGAG
GCAGGCAGATCTCTGAGTTCGAGGCCAGCCTGGTTTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACA
GAGAAACCCTGTCTCAGGAAAAAAAAAAAAAAAAAAGACAGTGAGGTAACAGTGGTTTAAGCAGCCAGTG
AATGAAACTAATCACCCTTTCAGGTTCCTTTCTACCTTTAAGTACCCTACTCTGACTTTGGAATTCATGC
TATGAGGCTGAGCAGTGCACCAGTGCCCACCTTTAATTCCTTTGATGGAGACAAGCAGATGTCCATGAAT
CCTAGACCAGCCTGGGCTACATAGTGAGTTCAGGGACAAGCCAGAGCTACACTGCGAGACCTTGTCTCAG
AACAACAATAATAACAACAAAACATAAAATAAGCCAGGTGTGGTATACACCTTTAATTTCAGGAGGCAGC
AGAAGAGGCAAGTGGAACTCTGTGAGTTCGAGGCCAGTCTGGTCTACGAAGGAGTCCAGGACAGCCAGGG
TTCTGAGAAACCTTGTCTCAAAACACCAAAACCAAAAAATAAAAAAATGAAAAGCCCATAAAACAAAACA
AAGGGGGGAGAGAGAGAGGGAGGGAGGGAGGGAGAGAGAGAGAGAGAGAGGGAGAGAGAGAGAGAGAGAG
AGAGAGAGAGAGAGAGAGAGAGAATAATTCATACTGTGGTTATAAAGTGGGAAAGAATCTTTGAAGGCAG
GATGATTTGCTGAACCCCCAGAAACTGTGTCTATAATGCCAGCTTGGAAGATAGAGGCAGGAGGATTGGA
AGCTCACAGTGGCCTGCTATGTAGTGAGTTTGAGACCAGCCTGAGTCACAGATGATCTTGTCTCAAAACC
CAACAGCAAACCTCAGCAACAAAGCAAAACAGAGAGACGCCACAGTTGACATGTGGACATCCATCTTACT
GGCATTTCTTTCCAAGCCTCCCCTGACTTTGGAATAACCATTCTCTCACTCAGCTTCTCTGGGCTGACAC
TGGAGCCCTGTTCCCTGAGGAGCTAGCCGGGTCTCTTTAGAAAGGAAAGCAGACCCCCTAGGGAAAGATA
TCCCCTAGGAGGAAGCAGTAGAGTTTTCTCACAGGCTGTCACTGTATCTTTAGCTTACTGGAGAACAGAG
AGAAGCCAGCTCACACCCACCTAGTCTCAGGAAAGGAGAGGGGAGCTAGGCTTGGAGAAAGCTTGGTTGA
CTCCACGGAGAAGGAGCCTGCATGGGGTCATTGTGAGCATACCGGCCAGAGAATGGCCAGGCTTATGACT
GGTTTGGAGCATGGGGGGTCTCGGTGGGATGAAGTGGGGAATAGGGGAATAGGGGCCCTACTCAGCCCAT
TTCCTTTTCCAGGATTTCACTTGCAAGTCCAAGTCTTGCTTGGGGTCCATCATGAACCCCAAGAGTTTGA
CCAGAGGACCCAGAGACAAGCCTACCCCTCTGGAGGAGCTCCTGCCTCATGCCATTGAGTTCATCAACCA
GTATTATGGCTCCTTTAAAGAGTAAGTCTGGCTTTCGTCAGGGTGACATCCCCTCTGCCCTACTCCCTGG
GGCTGACCTGTTTCCTACTGTTCCACTCTGATCGGTGAACTCTGCAGTCTCCAGACTTGATGGGGCTAAA
GTAGGCTCAGGCACTGCAAAAATGTCTGAAAGTTTGGAGGAGAGTGTAACCGGTTTTGGATTCCGGCGGG
CCACCAGGTCACTGCTGAGTCCTGGAGAAATCCATTGGGTCTCAACCTCCTCATCTGTAAAGTGGGCATC
TTATAGCATGACTCACTAGACAGCCATGAGATGTGTACAAATTTAGAACAGTGTTCTAGCCTAGGGACTA
TAATACAGTGACTTGGAATGAATCCTTTTATCCTGAAATAGAAGGACATTTAGAGACTGTCTGGTCAGGC
CTCTCCTCCTACAGATGTGAGCAAGATTCCCAGAGACAGCACTGGCATCAGCCTAGTCTAGCTAGAGTCC
GGGAGACCTGACTTGCTGGGGTGCTACATCTCTGGAGTGCTCTCATTTTATGCCATAATGCTTCCTTGAG
TCCAGTGTCCAGACCCCTTTATTCTTTGGGGTTTTGTTGTTGTTGTTTTGTTTTGTTTTGTTTTGTTTTT
TGTTTTATTTATTTATTTGGTTGGTTGGTTGCAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGC
TGGCCTCGAACTCAGAAATCCGCCTGCCTTTGTCTCCCAAGTGCTGGGATTAAAGGTGTGAGCCACCACT
GCCCGGAGAGACCCTTTATTCTTAAGCCCACTAGTCAGTCACTCCAAGCACCAAGTATTGTCCAGCATGA
AAGGCAATTGTTTTTCATTGCCAGTTGCCAATGTTGTTGTCAATGCAGTTGCAATCATGGCATGGCTGTG
TGGCAACTCTTGGTAAGAGTTGGGATCCAAGGAGCCATCTTCTGAGGTTCAAGCCTTGGTTTTCTTGCCT
CAAAATATCTATTATAAAATACCCACCTTGTAGGAGTTTATATGAAGATTAAATGAATTAGAGGCTTCAG
AACAGTGCTGGCCTGTAATGAGTGCAACCGAGATCTTGGTTACCTCGTACGTACTCAAGCTTTTAAACAT
CTGTTTACTTGGTGGAGAATGGGGTACCTGAGCCACAGCAAGTTTGTGAAGGTCAGACAAGCTGTGGGGG
TTGGGCATCTCCATCCACAGTGCGGACTCCTGGGATTGACTGAGGTCATCAGACGTGGTATCAAATGTCT
TTACCTGCTCAGCCCCTCATTGGCCCCTTTACCCTCTGGCCCCTCATTGTTTTGGAGACAGCATCTCATG
AAATAATGAAGGCTGGCTTCTGCCCACAATTGTTCTTGAGAGTGCTGGGGTGACAGGTACAGGGGATGCT
GTCTAATTATAGAACATTGTTGACTGACTGATTTACCTGTCCAGTTGTAAGAATGACTGTCAGAGACAGC
GAATGTCTACTCTGGCAGCTGGGGACGGTGTGGCAGTGTCATTTTGTGGCACAGCCTCACAGAGCAGTTC
CACCTGGGGCGTGAGACACAGAAGGGTATCTGGTACCCAGAGCAGAAGTTTCCATTCTTGGGGAATCCCT
GTTCCACCTCTGTACAGCCCTGGAGTGCCAGAGCCGGGAGGGCGCTTGACAAGAGGTCTCTATCGTCTAG
AGTAGTGCTTCCCAGCCTTACCTGGGAGCTGGCAGAACAGTGATGACTGGTCCCCCCTGAGCCAAGGTCA
ACCATCTTGGGAAAGGCTGTCTGTAGATTTGTGAAGGGTGGAGGCCTGTGGGGGCACTGTCTTTCCTGTA
AGCATGTATCCGGGGCTTTGTGGCATTGAACTTCAGTTTCCCAGATCTTCCCACTCTGTTGTTAACAAAT
GGCTGTTTGGGGGGTCTGAGATTCTAGATAGGCTTCATCTGGCCATTTTTTTTAATTTGGCACCAAGCAC
ATGAAAAACTCTGAGTTGCTGGGTTAACTCTGAGAGCCAGGTGTGGGCAACACAGCTCTCTATAGATAAA
TAGGAAGATCTTAGGAGGACTCCAAGTTCAAGGATTGCGTGGGTGACAGGGTGAATACAAGCCAGCCTGA
GCAACATAGAGGGAACATGTCTCAAAATAAACGGTTCAAAAAACAAGTGCTGGGGCCACAGCTGAGCATG
TAGCTCAGTGGTAGGATAAGCCTGGTTTGGCGCCCTGCTTTTCTTTTGAGGCAGGTACTCACTATGTTGC
CCAGGCTGGCCTGAGTTTACCTAATTTACACAAACTCATTTGGAAATTACAAGTGGGTGTGGAGAGACGA
AGCCTGCCTATGGTTTGCTCCACGTGCCGAGTCCAGAAGGCCAGAGAAACAACTGTCTCCCTCCTGACAC
TCCTGTGAAAGACAGAGGCTCCTGTTTCTTCTTGCTACCATAGTTACTGCGCTTTTCATTACAACAAGAG
GATGGCTTTGCTCAGGACCTGTCCACTCCTTAGAATACAACGGGAGAGGCCAAAACTCTTCCAAAGAAAT
CAGGCCCACTCCTGGGTAAATGACACCAAGTTTTCCTCTTTCTCCTTGAAGCGGTGTTGCCAGACCACAG
ACACTGCTGTTTTCGTGTTTGGGTAGAGTGGGGTGTATTTCTAGAATCTTCCTCTGACGACTAAGGACAC
TCACCTCACCCAGAAGTTCCCCCTCACAGGGATCACTAGAGACAAACATCGCTGAATGTTTTGTCTTCAA
GTCTTTCTGTAGTCAAAAAGGAGTTGCCAAGACCCTCCGCCTGCCCCGAAGGCTGTTCATTCCAAGTCCT
CCTGCTGACCTGAATCCCAAGTTCATTCTTTAAATAATTTTGTTTGTTGCCATTTCGAAAGCCTCTGGTG
GTGCTGGTTGGAAATCTCAAGGTTGCTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
TTCAAAGTCTTTGCGACCAGGCTCCTCTCCCTTGGAATTATTTTGAAGTATGCTTAAATCAGTGAGTGTA
GCTCTGTTGGGTGGGCTCTAGGGTCCTTCAAGTTGAGTCCTGACCTCAGGTCTTGTCCGGGCCTGCCTTG
AGTTTTGAGGAGCCCTCGAGGCTTGCTTCTCCCCTTGGGTGGAAGGTGGGAGGTCCTATCCACGACAGAC
AGTTTGGAGCCTGGGGCTGGCAGTGAGACCACCTGGAACTATGTCCTCTCTGTTACTAGGCTTGTGTTCT
TGAGCCTGGGACTGTATACTCTAGAGTCTTAAGACTGCTGAGCAAACACAGCAGTGCCTCCTAAGTGACT
TGTGGTGGCATGTAGCTGCACTCTCAGGTATTTGAGAGGGGGAGGCCAGAGGGTTGGAGGTTCTAGGCTA
GACAAGACCTTGTTATGACTATAATAATCAGCAGGGTCCAGCGTAGGAACATAAGTGTGGTTTTTCTCCC
AACAGGACTCAAGATGGAGATGGCAGAGTATTAAAAATCGGCCCCTAGTAAGCGAAGAGTGGGTCACAAA
GCCAGCCCTACCGAGGACAATGTCCCAAGGGGCACAATTTGCCCTTCTAGTGTCCAAAATCAGTTCTTGC
TTTCTGGGGATTGAATTAATCTCCATGTAAGGTCTTGCTATTGTTGGTCTTCCTGACAATTTCCCGTCCC
ACAGGGCAAAAATAGAGGAACATCTGGCCAGGCTGGAAGCTGTAACAAAGGAAATAGAAACAACAGGAAC
CTACCAGCTCACTCTGGATGAGCTCATCTTTGCCACCAAGATGGCCTGGAGGAATGCCCCTCGCTGCATC
GGCAGGATCCAGTGGTCCAACCTGCAGGTGGGTCTTACCGGCAGAGCAGAAGGAGGAGCAGGGGTGGGCA
GGCCCTGAGACAGAGCAAGTGGCCAGGGCTCTGCAGACTACAGCTAAACCCTGAGGTGAGGCATCCCAGA
CCCCCTTCTAGGAGCCTAGAATCTTATTTTAGCACCAGGGTCTCTAGATGACCCACCCTATATAAAATGT
TGGGGACCACATGCCATCATGAGACAACCAGCAACCTTGGATGTCTTATTCCTGAGGCCTTGGTGCTACA
AATGTTTGCCTCCAGTGTTTGCGATCCTGGCCCTAGAGAGCATTTTATTTATCGTGGCCTCCTAGGCCAT
CGTCTCCTTGGAGGGTCATTTGGGCAGGGGTGGGGGGTTGGGTCCTATTGAGTGCACTTGGACTGAGAAA
ATCCAGGAGTCTCAATGGTGGTGCACTCCCATGGCCAAGATCCAGGCTAGGGCTGTGGGGATCCTGCCTT
TTGGTTCTTTTCTTCTTTTAAGATTTATTTATTTATTTTATGTATATGAGTACACCATTGCTCTCTTCAG
ACACACCAGAAGAGGGCATCAGATCTCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGA
ACTCAGGACCTCTGGAAGAGCAGTCAGTACTATTAACCGCTGAATCATCTCTCCAGCCCCTTTTGGTTCT
TTAAAGCCAGACACTTTTGGTAATTTGGTTTTACTGGGGTCTTCTGAATGGGATTCTCTGATCCCTTGTC
TCAAGAGTAGGGCAGCCTAGAGAAGGGCACAGGGGATGCAGAGAGGAGGGTGTGACCCTGTGAGAGAAAT
GCCCTCTGTCCCCAAGGACTTTGAATCAGCCCCAAGCCTCTGTCCTCACAGTCTTCCATGGTGTGCCCTG
TGCAGGTCTTTGACGCTCGGAACTGTAGCACAGCACAGGAAATGTTTCAGCACATCTGCAGACACATACT
TTATGCCACCAACAATGGCAACATCAGGTGAGGCACCTTTCCCAGCTGTGCTGGGCCCAACTGACTACTC
ACTCTCAGGAAGAAGATGCTGGAGAGCACAGGAGAGGGGCCTCTGGCTCCAGGAGAAACTAAGGAGAGAG
AAAAGAATCCAAGACTTAGCTTTTCCAGGACATGAAATGGCAGGGCAGCCTCCTCCCCATTAGTCTCTGT
TATAGCCCAGCTCTTCCCCGACCCCTTTCTAAGTTAGAACCTCAGGGCTGTTACATCTTTAGGAAATTAC
TTTGAGCAGGCCATGGTGACACATGCCTGTTATCCAAGCACTTGGAAAGGTGGAAGCAGAAGGATCAAGG
GTTCAAAGCCATCCTTGGCTACATAGCACGTTCAAGGCTAGCCTGGGCTCCATGTGTTCCTGCCTTAAAA
ACAAAATAAAACCAAAACAAAGAAAACCATTTTCTTAGTTGGGCATGGTGGTACATAACAGCAATTTCAG
CAGTGGAAGGCTGAGGCAGGAGGATTACCATGAGTTCCAGGCCAGTCTGGGTCACATTGACAGACCTTGT
CTTGTTTTTAAAAAGAATCTACTCAGAAGGCAGAGGCGGGTGGATCTCTGTGAGTTCAAGGCTAGCCTAG
TCTATATAGTGCTCAGTCTGTCATTCACTGTCATGCAAAAAACACGCACCTAATGGGGATTCAGCACAGG
ACTAGGCCAGGGGCCAGGGAAAAGGAGGTGACAGGGTGGGATACAGAGGCGACTGAGACCCTAGAATGCA
GCTCCATCCAGCTGCAAACCTATGGTGCACAGCCACCAAGGGTGAGAATAAAAGGATCCAGCAGCGAAAG
CCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAGGGACATGAGGCTTCC
CAGAGTGGTGGCCCCTTGTTGGTCTTGATAGTTAGGGAGAAGCTGGCAGGAGGAGGAAGAGGAGGGTGAT
ATCAGGGTGATTGGGATGCAGGGAAGAGTGGAGGGAAGTTGGCTGGGAACCCCTGACCCACTCACCCCAA
CCTGGGACAGTTGATAAACTACTCTGTCCCTGTTCTGGGGAAGGTCGGCCATCACTGTGTTCCCCCAGCG
GAGTGACGGCAAACATGACTTCAGGCTCTGGAATTCACAGCTCATCCGGTACGCTGGCTACCAGATGCCC
GATGGCACCATCAGAGGGGATGCTGCCACCTTGGAGTTCACCCAGGTACTGACTCAGCCTCTCCTAGATC
CCTGTGGGCAGGGGACCCTGAGCAAATGTGGGGCCTGGCCCTGCAGACTTACTGGGCCCCTCTCTCCCTC
AGTTGTGCATCGACCTAGGCTGGAAGCCCCGCTATGGCCGCTTTGATGTGCTGCCTCTGGTCTTGCAAGC
TGATGGTCAAGATCCAGAGGTCTTTGAAATCCCTCCTGATCTTGTGTTGGAGGTGACCATGGAGCATCCC
AAGTAAGTCACCCAGGGTACGGGTTATATGCATGCAAGCCCATCTCAGGCAGCACCCACAGGACAGATGC
TGCAAGGCCTCTTTGCCCTCTGCAGCAGCTCTGGCCTTTCCTCTCCAGCCTCGCTTTATGTGGAACTGCT
GATGGTTACCAAGGGAACAGCAGCCTAGCAAACAGGGTCCTGGGAGAGTGGGATGCAGTGACTTAAGGAA
AGCCCCTGAGCTTCTGTCTGAGCAGAACCAGGCTTTTTTCTCACCTCCCAACAACTTCTTTAATTTTTTT
CTTTTATTTATTTAGTGTGTGTATTTGAATGTGTGTGCTTGTGTGTGTACCTGTATGTGACTGTATGTGT
GCATAGGTGTGCATGTTTGTGTGTGTGTGTATATGATATGTGAGGTATATGTGGTATGTTTATAATCATG
TGTATGTTGTTTGTATGTATGGTGTGTGGTGTATGTTTATGTTTGTAAGTGTGGTGTGCTTGTATGTTTC
TGTGTGTGATGTGTATGTGTGGTTATGTGTGTGTGTGTGTGTATGGTGTGTGTGTGTGTGCTCAGAGGTC
ACTTGCAGGAGTCAGTTGATTTGCTCCTACCATATGGGTCCTAGGGACTGAACTCAGCTTATCTATCAGA
CATGGTAGCTAGTGCCCTTACCTGCTGAGCCACCTCAATGGCCTCCTCCCAGCTTTCTTAGGAAAAGGAC
AACCCTCCCAGACAGAGGAAGTGTAGGCTCACAAGGACTTTAAGAGTGCAGCTGGGCCAGGCAGTGGTGG
CGCACGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCGAGGCCAGCCTGGTC
TACAGAGCGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTTGGAAAACAAACAAACAAACA
AACAAACAAACACGAGTGCAGCTGGTTGAACCTCTGCAAAATGCCTCCATATGTGGAGGTTTACCAGCTA
GCAGGTACTGACAGCTCCTGTCCTTTGAACACACTGAACTCAGTCCTGCCTCAGGACCTCTGTCCTGCTT
CCCTGCCTGCTATGCCCTTTCCTCGGAATTCTGCAAGGCTCCAGTTTTCCCATCAAAAGGTATTCACTAT
GACATCACTGCACCTCGGAGAAGTCTCTCCTGATCTCCTATCTTCAACCCCACACCACACTTCACTTCGT
TTCTCATTTTCTCACTGGTGTATTTATTTACCCCTCATAGTCTATAGTCAAACGGTCTATAGTCTGGATT
CTGGGCTGCAACTTTGGGAATAGAATCTTACTTTTTATCTATAAAGTGCAGGGCACGTAGTAGGTGCTTA
ATTATAGATATTTAGAATGTCTAACTATACAATGAATGATACTATGTTTGCAATAATCCCATGAGTTTGT
TGTTATCGACTCTGTGTCTATGATGGAGATACTGAGGCTCCGAGGGGCTAAAAGGACCCTGGTCAGTGCT
TGAACCCCAGCTGTCTGGGTGCCAGAGTACTTTGCTGCTACATTATGTTGGAGTCTCAGCATTTAGTCCG
ATATACACCTGACTTATTAGCATGAATGAGTGAGTAAAGAGATGTCTTCTACAATAAATGACCCTAAGTG
TTCCCTGTTATCTCTCGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGACT
GAGATCATCTCTTCAAAAGGACTCTCTAGAGCTCGGGCTATGGCTCAGCTGAGCATGATTGCTTAGCATG
CTTGAAGCCCTAGCTTTGCTCCCCAGCAGTGCATAAACTGGGTGTCGGTGATACATGCCTATAATTCAGG
CCCTTGGGAGATAGAATCAGGAGGATCAAAAGTTCAAGGCCATCCTCAGGTACAGAAAATCAAGACCAGT
CTTGGAAATGAGACCCTGTCTCAAAACAACAAAGAAAAAGAAAAGGAAGGATTTCTTTGGTTGATCCTTT
TACTGAAGTTGATTTTTTTTGATTGGGGAGAGAGGGCAATAAAGATGGCCCTAAGTGCAGCTGGCTCTGC
CTCCCTGCCAGGTACGAGTGGTTCCAGGAGCTCGGGTTGAAGTGGTATGCACTGCCTGCCGTGGCCAACA
TGCTACTGGAGGTGGGTGGCCTCGAATTCCCAGCCTGCCCCTTCAATGGTTGGTACATGGGCACCGAGAT
TGGAGTTCGAGACTTCTGTGACACACAGCGCTACAACATCCTGGAGGTGATGAGGGTTACCAAGCTGGGA
CAGCTGGAGGATCAGGATGGGGGACCCACTCCACCCAAACTGCCTCACCCTCATCACTTGGCTTCTATAG
GAAGTGGGCCGAAGGATGGGCCTGGAGACCCACACACTGGCCTCCCTCTGGAAAGACCGGGCTGTCACGG
AGATCAATGTGGCTGTGCTCCATAGTTTCCAGGTATGCCAGGTATCTTGGGGGTTGGTAGAGGTTTTGGC
AAGAAGGTCACTTGATCCAGGTACTTGACTGGCACTTGATGTCCCCTTTACAACATCATTCATTCATGTG
TGCATTCATTCATGCTTGCTGGGTGCTGAGACCCAGCATTATTGCTGGTCTAGCTCTGGGCAACTCCTGT
CCTCCCAAGGCTGCCTGCTCCCTGCTCCTGCACTTGAGCACTGTAGGCAGCTTGGCTTCCTTTGCTCCAG
GACATTGCTTAGAGACCCCTCCTCCCGGCTGCATCTCCAGGGCCTTCCTCCCTGCCCCATCATTCCCACT
GTGCACCAACAGTTCCTGCCAACCTCTGGCTCCTTCTTCTTCTCCTGCTCCTGTTACAGCCAATGAGACC
CATCTCCTCACACAGCCTCTTCCTCCGCCACTTGCAGCCTCCTTTCTGACTCCCTGGGACATCTGAACTT
CTTGCAGGGATCAAAGACTCAGGAAAAAAAGGGATCCTAACACAGGACCATAGCTCTCCTAAGCACATGC
CATGGTGTGTGTAAAGGTCAGAGGACAGCTAACAGGACCCCGTTCTCTGCTTTCAGTATATGGGTTCTAG
GGATCAAACCCAACTTGACTGGCTTGGCAGCAAGCACTTTTACTCCTGAGCCATCTCGCTGGCCCCCTTA
CTTTTCCTCCCACCCCGTTTCTTCTTCTTTTAAATCACCCCACGACCAGGTCCTTCCCTCCTTGAAGGCT
TCAGTCGTTCATGTCCTTAAAGAACCTCTCCTGGACCAGGCCACTTTGGGCAGTGCTCCCCCGTCCCCCA
CACTGTCTTCTTTCCTTCCAACTCCCTTAACTTGACTTTGTTGAAGTGGTTCCCAGCACCAGGTCGTAGG
TTGACGACCCCAATCTTTGTTGTTTCTTTCCTCCGCTGGAAATGTGAGCTGCGCAGTGCTCAGTGCTCCC
TGTGGCCGGGATTGTGACTGAGTTCACTGCTACACCTCTAGTCTAGCTCATGATGGAGGATTGGGAATTA
TGGAATGGATGGACAAGTGGAGGTGAGCCGCCCCCGCCCCCCCCACACACACACATGAGCAGGGGCCTTG
TGTGCTAGGTGGACATGGTAGCTGGTTCCTGTGTGAACTGAGCCTTCTCTCCTCAGCTTTGCTTCTGTGG
TTCTGTTTCCTGTGGAGCCTGGATATGTGAAGAGGTGGGGAGTCCAGTCAGGGTCATTCATTCTTTCAAC
GGGGATTTAACAAATGTTTGCCCCATGTGTGCCATGCATTAGCTATGGGTTCATACAATGTCCTCCAGGA
CTGGGACACTCTCTGGAAGATTAATTTTGTGCTCACTGGAGTAAAGGGTGGTGAGCTAACACAAAGAAGG
AAGAGCCCCACCCCCAGCCCCTGGGGCAGGCCCAGGCAGGAAGGACCACCCCTGGTTCATACATCCTAAC
CCCCAGGGACTCCCACCGCCAACCCTGACTCAAGCAGGATGCAGATGTGTCCCAGCAAATGCACAGAGAC
ATAGTCACCCCCTGATGATGTCCCTCTACTGCCCAAGTCTCCAGGCCATTCTTGTCACCAACTTCTAGGA
CACCCTTCAAAAAGAGATGGCCTTTTTCCCTTATAAAGTGAGGGCAGTAGACAGGTGTGATGGAGCACTA
CTGTAAGTCTAGCATTCAGGAGGCGGATGCAAGAGGATGGTGAGTTCAAGGCCAGCCTGGGCAACTCCAT
GAGTTATTTCTCAAAGAGGGAAACAAAACAAAAAATGGGAATAGCGCTTCCCTCGATGAGTCATTGCTAA
GATTAACTGAACGTGAGACACCTTTGGAGCTCTCTTGTTCCCGAGGGGTGCTCAGCATACGTGGTTGGTC
CATCTATGCTTGTCGGAGTTATCATCCTCTCCTGACATCCAAGGGCACTGTATATGTGCACAGATAGTAC
CTGGCTTACGAAGGCTCCACTTGGGAATTTTTTACTTTACAAGGAAGGATATGAAGGCACCGTGAATCTA
GAGCAACTCTACTTGGAGTTTTTAAAATTCTTTTTTTTTTTCATTTTATTTTATGTGTATGGATGTTTTG
CCTGCACCACATGTGTGTAGTACCTGGCCAGAAGAGGGCATTATATCCTCTAGGACTGGAGTTAGAGGAT
TGTGAGCTGCCATGTGGTTGCTGGGAATCAAACCAAGATCTTCTGGAAGAACAGTCAGTGCTCTTAACCA
CTGAGCCATCCTTCCAGCCCCCTACTTGGCTTTGTTGTTGTTTGAGACCTTGACTTCTCTCTAAAACTCT
TTTTTTTTTTGGTTTTTTATTTTGAGACAAGGTTACCCAGGCTGATCTTCAGTCACTCTGTTGTCTAGGC
AGGCCTTAAACTCAAGGCAGCCTCCAGAGTCTCTGTGCTGATGGGCCTGTATGGTTAACCGCATAAGGAA
TTTTAATTTCGATCTTTCCTGGGCTAACGATGATGGCCGGATGCTCTTTTGAAGCTGGACAGCAGCAGCA
AGCTACAGCCTCAGCCAGCTATGGCAGCTACAAGGACTAGAAGCTGACACTATAGTGTGTTGTACAGTCA
AGCTACGATGGTTGGGAGGCAAAGTGCTTTATCAAAAAGACTTGAGATGGAGTCTCATGTACCCTAGGCT
GGCCTCACACCGGCTATGTAACCTCTGATCTTTTTGTCTACCTCCGTAGTGCTGGGATGACAGGTGTGAG
CTACCACATCTGAGTCATTTGTTTGTCTTTTTTTTAATGTAGTACTGGGCATTGAACCTCCAGGTCTTGG
TGCATGCTAGACCAGCAGGCTATGTTAGCTGAGCTACACCCCAGTCCCCATTCGATGTGTTCTTGACTGA
GGATACTTTCATCTTGTGATGGGCCCATCAGCATGGAGCCCTATCATGAGTCAAGCAGCAGCTGTATATG
AGTTTTAAAGTGTGTGGTTTGACTCATGAGCCTGACATCAGGTGGCAGCTGCCAACCTTGCCTCATCCTT
CCCCAGGAATATCCCTTCCTTGGCCAACTTGAAACTATTTCTTAAGACTCTTTTCAGCTGTCCTGACTCC
CACCTCTCCTCTAGACCTATCCCAGACACTAGGGTTTCCCCCTCTGTCCCCTCTCTGTCCCCTCTCTGTC
CCTTCCAAGCAGCCTTTGCTTGCCTCTGTCACAAATCCCACTAGGCCTCATGAAAATAGCTGATTTCCTT
CAGCCTTTCCAAGAGCCTTGCTGTTTGCTGGCAATACATGGAGACTGTCTCTCCTCTGCCCCATGGTCTT
TCTCAGTACCTGACAGATGAACAGGAGCAGATAAGACCCTTTGAGAGCACAGGATGCTTCAGTGTTGGTT
CTCTCCTTCCACTGTGTGAATTTCTGAGATGAAACTCAGGTTGGGCTTGGTGACCAACGCCCTTACCTAC
TGAGCCCTCTCCCCAGTTGTAAAGAAATTTCTCATGGGTGAATGGATCTTCAGACTTTGATGGGGCCGCT
ACTTTTACTGGATCATCCCAGGCTAGTTTAGGCCTCTAGTTTGTAAGATGTGAATGGCTTGTCTTCCCTA
CACTCCGGTCCCCACCGTCCCCACAAACCACTCTGGTGGTTTTGTGTTTGCTTGTTTTGGAGACAGAATC
TCTTGGAGCCCAGGCTGGCCTCAAACTCCCTACGTAGCTGAGGATGACCTAGAACTCCCAACCCTCATCC
TGTACACCAGAGTGATGAGATTATAGTTGTCTAGTGCTTCTTGTATGCCAGGCAAGCACTCCACCAAGTG
AGCCTCCTTTAAGTTTGTATTTTCTTTACCTGAAACAAGATGGTGCAGTGTGAGCTGCCCACACCTGAGG
CTCTCTGTCTGGTGGCGATGCATGGAGACCTGTGCATCGAATTTCTCGATGCCGCTTTTAGGGTAGTGGT
TCTCAACCTTCCTAATGCTGGGACCCTTTAATACACTTCCTCATGTTGTGGTGACCCCAACCATAGAGTT
ATTTTCATTACTACTTTATAACTATAATTTTGCTACTGTTTGAATCATAATGTAAATATCTAAAATGCAT
ATCTGATAAGTGACCCCCAAAGGAGGGGGAGGGAGAAGACACATAGGTTGAGAACGTGGGAACCTCTCAT
GTGACCGCAGGCTGGCTGCCATATGTGTGACCGTGGTGATGGCCTAGGTCTCTAAGTCTGTGGACTTGGA
CCTGTGTGTGTTTCTGCAGCAGTCAGTGAGAACATGAGGAGCAGGGGCAGAGTCATCGCGTAGATCACTG
GCCCTCCAGTGCGGCACTTCCCGCTCTCCTCTGCTGCTGCATCTCAGTCCACTCCATCTTCTTCTCCTTC
AGAAGCAGAATGTGACCATCATGGACCACCACACAGCCTCAGAGTCCTTCATGAAGCACATGCAGAATGA
GTACCGGGCCCGTGGAGGCTGCCCGGCAGACTGGATTTGGCTGGTCCCTCCAGTGTCTGGGAGCATCACC
CCTGTGTTCCACCAGGAGATGTTGAACTATGTCCTATCTCCATTCTACTACTACCAGGTCAGGGAGAAGC
CTCCACCCACCTGCCCATCCCCGCCCCACCTCCCATCTTATAGCTCAGCTGTGGAGACTCTGTGGTGTGA
GGACTCAGGGCTGTACATGCCAGCTGAGTGCCACCTTGTGCTCTCTAATCTAGTGTACCCAATAAAAAGA
TACTAAAAGCAGGTGGGCCTCTACCTTCTGTGACCATGACAAAGGAAGCCAAGTGCCCACTGCTGTGTGG
CTCATGTGGGGCCCAGGAAGCAGTACCCTGAGCTTTGGCTAACCCTTGGTGGAGTGGGCCTCTATGAGTG
CAGTAGAGGGTCCTCTGCCTCTCCAGCATCCCCCGGCCCAAGCCCAGCCAGAACCCCACCCCCAACTCTC
CTTCCTTTCTGCAGATCGAGCCCTGGAAGACCCACATCTGGCAGAATGAGAAGCTGAGGCCCAGGAGGAG
AGAGATCCGATTTAGAGTCTTGGTGAAGTATGTGGAAGTGGGCATGTCCCTCTGTCTGTGCTGTGTCCCT
TTTACTGAGGCATCCTTCCTGACTCCACCATGAAGGGCAGGGCATACGGAGAATGTCCCTTTTGGTGTTG
GCTCCCAGAACTCTAGACACCAATTCTGAGACTGATTTGCTTCTGTAGTTCAGATTTGCCAAAGAGACTG
AGTCAGGATGCACAGGGGCCACCAGTCAGAAAATGAGAAGTAGAGGTTCTGCCCCCCACCCCAGGGATAC
CCCTGTTCCGTCTCTTCGGGACACTAACTCACCACTCAGCTGTCTGTGTATCTGTCTGTTCATCCCCACA
GAGTGGTGTTCTTTGCTTCCATGCTAATGCGAAAGGTCATGGCTTCACGGGTCAGAGCCACAGTCCTCTT
TGCTACTGAGACAGGGAAGTCTGAAGCACTAGCCAGGGACCTGGCCACCTTGTTCAGCTACGCCTTCAAC
ACCAAGGTACCCACTTAGGTGGAAGGAGTCTGGAGTGAGTGTAAGCGGCCCCTTGGGAGCTGGGGGCTGA
TGTTCAACGGTTGTCACACAGGTTGTCTGCATGGACCAGTATAAGGCAAGCACCTTGGAAGAGGAGCAAC
TACTGCTGGTGGTGACAAGCACATTTGGGAATGGAGACTGTCCCAGCAATGGGCAGGTGGGTGGCCGGAG
ACTCGGCAGAGAGGCTTGGGAGGACCAAGGAAAGGGTCCGGGGGACTCAGCATGACACTCTGGGTTCTTA
TGCCACTTTCCCAATGGCACACAGTGTTTTCCTGTGAATTGACCAACTCTTACTTGTATGTGTTTAGATT
ACTTGAGGGGATTCATTACATTTATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGCAAGTCCCACAGCTTGCATGTAGAGGTTAGAGGACAACTTGAAGGAGACAATTCTTACCTTCTGTAAC
ATGAGTCCCTGAGTTTTTCAGGCTTGGTGGCACTTTTACCTCCTGAGCCATCACTGCAGCCACAACCTAC
TTAAAAAAATTTTTTTTCCAAAACTTCTTATTTAACTGGGCATTGTGATGTACACTCGCAATCCCAGCAC
TAGGCAGTAGAGGCAGGAGGATCAGAAATTTGGGCGCAGCCTGGACTCCATAGGAAATCTTGACTCAAAT
TCTCCCTCAACCAGGATTCCTCCTTAGGGGCTGAGAGATGGCTCAGCAGGTAAGATGGATTGTGGCACTT
TCAAAGAACCACAGTCCAGTCCCAGCACCTATGTCAGGTGGCTCATAGTCTCCTATAACTTCAGCTCTAG
GGACTCCAACATTGTCTTCTGGCCTTCCTAGGCACTCACACTCTGTACATAGACACAATCTCTGACACAA
ATCTTTTTTGGGGGGGGGAGGGGTAAGGTTTGTTTGTTTGTTTATTTATTTAATGTAAGTGAGTACACTG
TACAGATGGTTGTGAGCCTTCACGTGGTTGTTGGGAATTGAATTTTTAGGACCTATGCTCGCTCCAGTCA
GCCCCCCTCACTCTGGCTCAAAGATTTATGTATTATTATATCTAAGTACACTGTAGCTGTCTTCAGACAC
ACCAGAAGAGGGCGTCAGATCTCATTACGGGTGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTC
AAGATCTGAGGAAGAACCCTTATTAAATTGCTCTTACCTTCTGAGCCACCTCACCAGCCCGACATAAATC
CTTTAAAAAGAATTTTTTTTTAAATGTATTGTGGCTAGAGAGATGGCTTAGCTATTAAGAGCACTGACTG
CTATTCCAGAGGTCCCGAGTTCAATTCCCAGCAACCACATGATGGCTCACAACTCACAATGTAATAAGAT
CTGTTGCCCTCTCCTGGTGTGTCTGAAGACAGCTACAGTGTACTCACATACATAAAATAAATCTTAACAA
ATGAGCAAAGACCGTATCATTCTCACAAAGGAAAAATGGGCCGAATATATGAATTTTGTCTAGCTACCCT
CATGCAGGCCTGCACAGCCTTGTGTTTTGAGCAGAGACAAGTATGAGCAAGATGTTAAGGCTTGGTTAGG
ACTAATTTGAGTTAGTCTCTCCTGTGTTGGAATGATGCAAAGGCCATCGAAAAGAAAGTGCATGAGAAGA
GATTACAAAAGGCTTAGCTTCCCACAGTGTGATGAGTCCCACACTGCCTGAGACAGTTTCCTGGAAAATC
AAAGTTATTTTAGAGAATCTGTTGTTTCTAGCAACTTCCAAGCAAGAAGCAAGGTTCGTCTTCTTTTTGA
GGAACCTTGTGTTTTCCAAAGCTAATTGGGGAGGCTAGGGACTTCTGGGTCTTCCATTGAAGGGAAGGGC
CCACGGGAGCTGGTTCAGCTGCAGTGAGGGGCAATCTGGACTTGTCCATTGGCTTTCCCTTAGTATCTGG
ACTCTTTGCCTGCTCAGTGTGGCCAGTGTTTACACAGACCATCATAGTAAAGGAAAGCAGTGTTTTTGTA
GCTCCTGGCCTGAGATCTCCGCTTCCTTGTCTTTTGTAGACTCTGAAGAAATCTCTGTTCATGCTTAGAG
AACTCAACCACACCTTCAGGTGAGGCATCTCCCATATCCCTGACTCCTGGGCCCCAGTCTGGGATCAGAC
CAAGGAACAGGGTGTACACAGGATGTCTTCTCAGACATGTCCAGGGGCAGACAGAGTTCCGGTCTGAGGT
TTGTCTTCTGAGTGGGTTGAGGAGCAAGATGTGGAAACTGAGTCCCTCTTATAGATTTGCAGGCCTAGAG
CCCACTGGTCACATGACCAAGGAAGGCCAGACTACCGTGTGACTCAGTGGGCATGGGTCTGTCCTTGCAA
ACCATGGGCATTGATGCTGGGCATCCTGAGTCTCCATGTGTGTCCTTCTTGGCAGGTATGCTGTGTTTGG
CCTTGGCTCCAGCATGTACCCTCAGTTCTGCGCCTTTGCTCATGACATCGACCAGAAGCTGTCCCACCTG
GGAGCCTCTCAGCTTGCCCCAACAGGAGAAGGGGACGAACTCAGTGGGCAGGAGGATGCCTTCCGCAGCT
GGGCTGTACAAACCTTCCGGGTGAGACAGGCTTTAATGAGTCTATGCCAAACACATCTGGGCCTGGTGTC
CTGGGTGTCATGGCCTCTGCTAGGTGTAGGGCGGGGGTATGGGCCTGGCATCTCAGGTCAAAGCTGCTTC
CCCAACCCTCACCCCACCAAGAAAAATGTCACTCTGGTGTTCTGCTTCAGGTGGGAGGTGATAGCTGTCC
AGCTCCGGCCCTGACCTTATGTCTCCAGGGCTCTGAAAGCTTGGCCACCTCGTAGTAGTGAAGTCTTCCT
TCAGTCTTCCAGCTCTGCAGAGGAAGGGACACAGGACACACTGCTGAGCTCTCGTGACCTGACTTTATCT
TCTCAAATCTTTACTCTCTGGGTTCCTCCCTGCTGGTAAAGGAAGCTTAACACTGAAATGAATGGACAGC
CATGGGAATCCCAGCAGACTTGATTCCAAGACTCAGGCCTCCTCTATGAGCTTTTCTATCAGGATAGGGA
AACCCCAGACATCCCTTGCTATGTGGGAAGGGATAAATATAAGATGACAGCTTTACTATCCAGGAAAATA
TCACTAACCACTGTGTGCCAAGCCTAGGATTCTCTGAGCGCCTCCAGCCCCTTTCAGATGGCACGTTGTG
ATTCTGTGATGCCGCCATTCGGTATTTGACCCATGGTGAGGGGTGGTGACGGTGACGATGAATGCCATTC
TGCCTAACTTTCCAGGCAGCCTGTGAGACCTTTGATGTCCGAAGCAAACATCACATTCAGATCCCGAAAC
GCTTCACTTCCAATGCAACATGGGAGCCACAGCAATATAGGCTCATCCAGAGCCCGGAGCCTTTAGACCT
CAACAGAGGTAAGAGCCCCTCTGCACCCTACTGGGCCTTCCCTGCTTGAGCTGAGGCTATCTGAGCCCTG
AACCAGTAGTCAAAGAATCCACCATGCCCTTCTAATGAAGGCAGTCACCTGGAACTGCCAGGCAGCTGTC
TCCCTGGCCACAGGGTCTGTTGGTACAGAGCTTCAGGCTCTGAGGGAGAGCGGGTCAGGAGCCCTGAGAG
AGTGGTAGTGGGCACAGGGAGCTGGCATCAGCAGGTGGGTGGTGGCGATAGCTGTGCCAGGCACTGTGCC
GGCCTCTTGGCCCACATGAACTGCCCACCAAGGGCTAAGGTAGCATGCAGGAATGCTCAGCTCAGCGTCT
GGGGCTTTCCGCAGCACAGCACAGGAGATCTGTACCATTTCTGTCTGTCTTTCCAGCCCTCAGCAGCATC
CATGCAAAGAACGTGTTTACCATGAGGCTGAAATCCCAGCAGAATCTGCAGAGTGAAAAGTCCAGGTAAG
GATGGGCAGGGATGCTTAGCTGGTACCCAAGATTGCCCGCGAGATGCTTCTTGTGACAGAAGTGTGGCCA
AGTACTCCATGCTCCCTGCACTGCTAGGGAAGGCTATAGCCAAACTCCTCTGGGAAACTCCAGAGTGCTG
AGGGTCCCCAGCTGAGGGGATGGCTTGTCACAAGCTGGCTGGTGACCACACTTGGTGGTGGGAGAACACT
TATCCTGGGGTAACTGGCAGTCACCCATGGCTCCCTTTTCTTCTGTAGCCGCACCACCCTCCTCGTTCAG
CTCACCTTCGAGGGCAGCCGAGGGCCCAGCTACCTGCCTGGGGAACACCTGGGGATCTTCCCAGGCAACC
AGACCGCCCTGGTGCAGGGAATCTTGGAGCGAGTTGTGGATTGTCCTACACCACACCAAACTGTGTGCCT
GGAGGTTCTGGATGAGAGCGGTGAGCCCCCGGCCCTGGACAAATCTCTACTTCCCTCAGTCCCATCCCTG
AGAACTGGGCCTGTGTCTGTCCTGATCTACTTCCATCCCAACTCATGGGAGAAGCTTTCTGGCCTTCACT
GGTAGTGAGGCCTCCCCCTAGCCTTGGACGAGGCACTGGAGGAGCAAAACCTGGCAGTGTCCATCATCAT
CCAGGAGAAAGGGAGTCAGCCTGAAACTTAGAGACAAGAGACTTGCATCCTTGGGGCTGTGAGAATACAG
ATGTGGTCCAGATGAGATGCATTAATAATAAAGTCCTTGTCTCTAGAAGGAAGTGAGGGGAGTGGAGAGG
AGTTAAAAGCAATGTGCTAACACGCACTAAACGTGCCCAGGGAACGGGCACTGTGCCAGCGATTTCCCAA
ATGTAGTTTTTACTCATTGCCAGCACAGACTATGTCATCACATCTGTTTCTCAAGAAGCAGCTGGAGGTC
ACAGGATTTGGACACTCGGCTGAGAAGTGGGAACCAGAGTTTGCGCCTGGGTCATGCCGTGACTCTCCGA
ATGCTGCCTCTTGGGACTCCTGAAGCCCAGCCACCACAATTTACATTGATCTGTTAATATCTCAGGAGGT
TGTAGCCATAAACCTCTCTCTAAAAAAGCAACCAGCCTCTGGGGCGTATATCAATCTCCAGTAGACTTAG
TCACTAAGTTTGAGGTTCACCTGGGGATGTCAGAGACTCAAGTATGGGCCACAGAAGGATTGCTTAGTCT
GTCAGGACAAAGCCAATATAGGTGCCCACGTGGGGGCAAATTTTCAGCCTACCCTGGCAGCCCTGTCTTG
GCACCAGCTCCTGATCTTTGCTCTTTCTGGAGCAAAGATGTCAGAGTCTTCCCTCCCTTGCAGATGGAGA
ATAGCCATCATCTGAGAGACCCAAGTAGGGCTTTCCCCACCCTGACACCTTTCTTTCTTCCATTCAGGCA
GCTACTGGGTCAAAGACAAGAGGCTGCCCCCCTGCTCACTCAGCCAAGCCCTCACCTACTTCCTGGACAT
TACGACCCCTCCCACCCAGCTGCAGCTCCACAAGCTGGCTCGCTTTGCCACGGACGAGACGGATAGGCAG
AGATTGGAGGCCTTGTGTCAGGTGAGGCAGGGGCAATTCAGTGGAAGGAATAGAGCCCAGAGCAGAGTTC
AACCTCTGAGACCATCCTCCTGAGTCCCTAGGTGTGCCTTGTCCGTGTCTGGTCGGGGCTAGGCGTCGTC
TTCTGCCCCGTCTCTAGTACACAGCAGTATTGAGTGAATGAGTTTCTCTGGGAATCAGTATGCTGTAACC
TGTATTCATCACATGTCCCATTTCTGTGACTAACTGCATGTCAGAAGGAACTTAAGGGAAGGAGGGTTCA
TTTTGGTTCATGAGGGTCCAGTCCATCATGGTAGGGTGTCATGACAACAGGAGCATGAGTGACAGATCAC
ATTGCTTCTGGAGTGAGAAAGCAGGAAAGGTGGACACCAGTGTCCTGCTCAAAGTCCCAGACCATAGGAA
TTCGGGGTGGGTCTTCCCAGTCAAACCTCTAGAGAAACACCTTCACCAAGGTGTGTTTCCACAGTGATCT
GAAATCCAGTCAGGTTCACTGTGAAGAGTAACAGCCAAAGTGCACTTCCTTAGAACCTGACTAAAGGGGG
AAGCTCTATGGATGGCCTTTGTCTTCCCAAGTGTGGACCACGCTTGGTGGTGGGTAAATGGGGGTACTGG
CGGGTGGGGAGCTGAGTTAATGAGTTAGTGGGGTGATGCATTAAGTCACTTGCGACTTTAGAGTCCATGT
CTTTATAAGAGTCACTGGACAGCAAGCCATGGGACACATTGTCATTGTCTCAACAATCCTATATTAATGG
CTGCATCCACCTAACTCCACAAGGATTAGGGTTAAGCTAAGGAGGGACATCCAGCCATGACTTGAACTCA
GATCCAGTTCGTTCCAAGTTACAGTATAATAGAGAAATTAGCCCAGCTGGGTTGGGTGGTGCATTCTTGT
GATCCCAGCACACAGGAGGCTGATGCAGGAGGATAGTGAGTTAGAGGCAAGTCTGGGTCAACAATGAGTT
CAAGACCAACCTGGGCTATGTAGCAAGACCATGTCTCTACATCCTCCCCAAAGAAAGAGGACACTGAGCC
CTGCCCATGAGTTGCTGATGTTGGGAGGCTTCTGTGTTTTGATTTTTGTCTTTGTTTTGTGGGTACTAGT
GTTTTCCTTCAGACCTGCACACATGCCAGGCTCTACCACTGAGACAAACTCTCTCTTCTCTGACTTTTAA
TTCTGAGGCAGGGTCCCACTAAGTTATCCAAGCTAGCTCTCAATTCGGTCTCTAACCCCTGCTGGTCTTA
AACTTGGAATTCTCCTGCGTCAGCCTTCTAACTAGTTGGGGTTGCAATCCTGCCCCACTGTCATCTTAAT
TCTATTCATAGTAGAGTTCTAGGCACAATTGAGAAATGCTACTAGCTGCCCCAGGTCCTTGCTTCCCGTC
CACCGGAGTAGCCTCAGCACCCTGGATAGCGCCCGTCTTTTGCTGCGTTTGTTGAAGGAATGAATGGATA
CTGGGTGGGGCAGCTCCTACACGCACGCAGCTCCTACACGCACGCGCGCACGCACGGCAGGTCCTCCGAT
CCCTCACACTGCCCTTGCCTTTCAGCCCTCAGAGTACAATGACTGGAAGTTCAGCAACAACCCCACGTTC
CTGGAGGTGCTTGAAGAGTTCCCTTCCTTGCATGTGCCCGCTGCCTTCCTGCTGTCGCAGCTCCCTATCT
TGAAGCCCCGCTACTACTCCATCAGCTCCTCCCAGGACCACACCCCCTCGGAGGTTCACCTCACTGTGGC
CGTGGTCACCTACCGCACCCGAGGTGAGACAGGCAGGCTCTGGTGTCATCTGCACCTGTCCTTCTAGGGA
CAGCTGTCCTCGATTGCTTTCTGTTGCTGTGACCAACACTGACTGCCACCACTTTAGGGCAAGATCCAAT
TTAGGGGTTTAACTTAAAGTTTACAACTGTCACCAAAGGAAGCCAAGGCAGGAACCACAGAGGAACGTTG
CTTATTGGCTCCCTCTGGCTTGCTCAGCTGCCTTCCTTACCAGCTCAGCACCACCTGCCCAGGATCAGCA
CTGCTCACAGTGGGCGATGCTCTTCTCCATCATCAATTAGCAGTTAAGAACGCACCCCACAGACATGCCG
ATAGGCCTATCTGATGACAAGCCCTCAATTGAGGGTCCCTCTTCCCAGGTGTATCAAGTTGATGGCCGAC
ACTGGTCACCACAACAGCTGTTCAATACCTTGGGGCTTTGTCAGCTACATGCTGCAGTGACAGCCACCTG
CCAGAGTTGTCAGTGATATAAGATGCTGTGTAGGGCACAGTGTCACAAAGCAGGAGGGAAGCTCGCTCCT
TTGTTTACCATTCAGACCTGTAGGAGATGGCTTCCATGTGTAATTTTACGAGGAAGGAAAAGAGTCATGG
AGAGGTTAACCCACCTGTTCAGAGACACCTGTCATTAACTAGAGACACAAATTCAGGGAGTTTACCTGCT
GAAGCTAAGAACTGGACCATTGTTACAGTCTCTGGCTGTTGAATAGGCTCAGACGTGAAAAGCCTTGAAT
GCGTAGGGAACTCAGTTACTTTTGGTCATCCTAATGATGCAGGTGGATGCAGGGAACATTTTCTCAATTT
TTTTCATTCCCTCAGTGTTCCCATCCTGTGATGGCCGTGTCTCACCTCCATGCCAGGGGCCACACTGGGT
GTGGAAACTGCAGACAGGCCCTGCCCTCACAGAGCAGCCAGAAAGTCAAAAAAGAGTTCAGGCATGGACA
TACTTGGACATATGTTTGTGTGGGTGCTCAGAGGCCCCCGGTATCACGAGGGAGGGAGTGGGGTGTGTGT
GAAGCTGCCCTAGACTAGGAGGTCAAGTGTTCTAGGCTTGGTTCCTCCACTGGATGGACCTCCCTGTTCT
AACCACTGTAACTGAGCTCTGTTTGAGCTGGAAGGGCTGGAAAGCGTCCACATCTCCGAGATCTCTCTCC
TTGGCCAAGAGCGCAGTTTGTAAATACTGCACAGAGCCTGTGTCTGTCCATCCTCACTGACCATACCACA
CCATTGAATGAGAGAGGAGATGCGGGTGAGCCCTGACAAGGCTGGTTCATGCAGACCAGAACCAAACAAC
TATTCCAGTTGCTAAAGGCCTCCCTTATGTGTCAGGCCATGGGGAAGTGAATGTAACCCAGAGGACACTG
GGGTACCCACAAGCCCAGGAAAAGGCTTCTGGGGAAGATGGAGATGCCAGAATAGACAGGGGGCGTGGAA
GACCACCCCTCACCCAGAGTTCCTATTGGCAAAGGCCTCCTTCATGAGGACACTCAGCAATGGCCAGCCC
TGGGGATCATTTCACACTGCGTGGGCAGAGCCTTAGTGGGGAAAGGATGTTGGGAGGGTCCCTAGACAAG
CACTTGGGAGATGCTCTAGCGTAAGACTGAGGAACCTGAGCAGGAAGTTGAGTCCACCATGACTTTAGTC
CCTTGCTCCGTTGCCTTCTGTGTGACTCAGTTTCTACAATGGCAAAGGCGCAGTTGCTCACCAGGCCCCT
GACTGCCTTCCCTGATCTGTATAGACAGCTGGGAGAAGAGGTAACTCCTCATTCAGGAAGCAGCCACCCA
ACGGGGCTTGACACTGCAGGAAGAACACCCAGCCGGGTTGCACGAAGCCCACGGGGCTTGTTAGTCAGGC
CTGTGTTTTCCTTGCAGATGGTCAGGGTCCCCTGCACCATGGTGTCTGCAGCACTTGGATCAGGAACCTG
AAGCCCCAGGACCCAGTGCCCTGCTTTGTGCGAAGGTAAGCACCCACTCGGGGCCCTTTGGAGCCCCTAG
CCCCTGGGGATTCTGCAATGCAAGGGAAGGGTCTAAGACTGAGTCTCCTCTCGGCCCATAAAGGTTTAGG
GGGTAATTCTTTGTCTCCTCGTTTTGCCATCTGGTTACAAGGTGTCGTGGAGTCCATGACACAGATACAG
TGATGCCTGTGGTGGGATCCAATGAGACTGGCCCAGTGTGGAGAGAGGCCGTTGCCTGGGCTGGGAAGAT
GAAGATGTGCACAGGTCCCCGCCAGGCGCTTAGGTCCCCAGCCCTATCCTGCATGTGCTCCTAGCCTGGG
ATATCTGCAGACCAGGGTCTTTCTCCTTCTGTCTTCCTGCAGTGTCAGTGGCTTCCAGCTCCCTGAGGAC
CCCTCCCAGCCTTGCATCCTCATTGGGCCTGGTACGGGCATTGCTCCCTTCCGAAGTTTCTGGCAGCAGC
GGCTCCATGACTCCCAGCACAAAGGTACAGCTCTTACTCAATAGGGAGTGTGCCTGTCACTGTGACCGAC
CTGATCATTACACAGGCACATCAAATTACTACACCATATCCCATAATCAGGTACACAGATGTGGAGTGTT
TGTGCTCTGCCCACTCCACGTCTCCATGTTTCCTTGTTAATTTTTAAGGTATCTAGAGGAGTGTGTATGT
CCTGGTACGCATGTGGAGGTCAGAGGTCAACTTGTTTTACTTCTTCCAGGAATCAGATTCAGCTTGTCAG
TCACGACGGCAAGCACCTCTCCCTGCTGAGCCATCTCATCAGCCCATTTTGCTGGGTTTTAAATGCTTGC
CTCCTTGCTGGACCATCCATTAATAACACAGAGATTAATAAGCCTGTGTAGGGGTCCCCAGAGTGAAGGG
GGAATGAGAGTAACAGACTTTAGATGAAGACATTTTCTTTTCATTGGAGAAATAGCTGGGAAGGATGGAC
AGCTCTGTCTGGAATCCTCCAGATGAGGCTTTTCTGGAAGAAACCCTTAGCAATGTTTGACCTTCAAGAT
GCCTGAGGCCTCTGCACCTATATTTGATGTAGCATCTGGGTCAGGGGAGATGATTTTCACAAAAGAAGGC
CACGAGTTTCTGATATTCCACTAAAAGAATGTCCTTAGCATGTTACACACACACACACCTATACATATTT
GAGACAAAGTCTTGCTATTGTAGCCCTGGATGAACTGAAAATTCCCTATACAGAGGAGGCTGTCCTCAAA
TTTGCAGGAAATCAATCCTCCTGCTTCTAATTCCCAAGAACTGGCTATAATAATATTTTAAAAGAAGAAG
TAAGATTAAAATAGAATATGTCAAGTAAAAGAAATAGCAATGTGTAGACATAATCAGAACTGATACTTTT
TTTTCCTGAACCATGTTTTTTAAGAGTGCCACCATTAGCTATAAAATGGGAATAATGCCAGATCAGAAAC
CTACCCATTGGGTACCTGGTGCTCACAGGTTGCCCTCTGGTGGCCGTTGTGGTAACTGCAACCTTCTGGA
TCTTCAAGGGCTCAAAGGAGGCCGCATGAGCTTGGTGTTTGGGTGCCGGCACCCGGAGGAGGACCACCTC
TATCAGGAAGAAATGCAGGAGATGGTCCGCAAGAGAGTGCTGTTCCAGGTGCACACAGGCTACTCCCGGC
TGCCCGGCAAACCCAAGGTAAGTGCAGCCACCCAGCAAGCTGGCAGAGCACGCATGATGGCCCCTGCCCA
GGCTATACAGCCCTTCCACTCCAAGCCTGCAGTGAAGACAGTTGCACAGTGGCAGGGAGAGATGAAGAGC
ACGATGACAACATCAAGCTCACTGTAGTTTAATCTCCACAGTGCAAACACCTGCAGGTGCCCAGCATCTC
TGGGACGCAGGGCTGGGCCGGGCTGGCTTTACACTTAGTTCAGTAAGACTGGAAGGTGGAGCTGATTCCC
CCCAGGGCCCTCAGGTTGGCACTCTGCTCCAGGGGCATGAACAGTGCCTACACACTTTCCATTCAGGACC
ATGTGAACATTCCCTGAAGACCCCATTTGCATCCTAAAGCTGGACCACTGGTCATCTTAGATGAAAAACA
AAAACAAACAGACTCCTGACTGACTGCAGGACCTTCTTTATAAATGGAAAGCCTAGGAGCCTATACCTGA
GCTACAAATCAGAGCTGGGGCTAATACATACTGGGAAGCCCCCTCTTTCCGGGGTGTAGACAGGCAGATC
TCACAGCCCTGGGCAGAGCTATAAACTTGATGTCTCTAAAGTATGGTGACCTGAGGGAAGCTTTACGGGT
TCCATTGGCCAGGAGGACAGGATACTTGTCTATCCTGAGCTGAGGTCCTGACTGAAGCCCTCAATGGCCC
TCTTCCCCTCCCCATGGCCAGTGTGCCTGACCACAGTGACACCAGCATCCAGGGCTGGTCCCACCGTGTC
CTCTGCTTTTAGGTCTACGTTCAGGACATCCTGCAAAAGCAGCTGGCCAATGAGGTACTCAGCGTGCTCC
ACGGGGAGCAGGGCCACCTCTACATTTGCGGAGATGTGCGCATGGCTCGGGATGTGGCTACCACATTGAA
GAAGCTGGTGGCCACCAAGCTGAACTTGAGCGAGGAGCAGGTGGAAGACTATTTCTTCCAGCTCAAGGTA
TGGGAGCTGGGGTGGGACCCTCCAGAGGGGAGTAGACCAAGGTCACAAGCCTTTGGAACTAGGACCAGGG
ATGCAGAACTGGGGTGGGGGAGGTGTGTGCCCTGAGATCCTCGGCACCACTGCAGAAACCTGGGGCCGGC
TGGGCTCCTCAAGCCTGGTCTTTCCATGCCATAGTGGTTGCCAGCTAGCTGAGAAATCAGGCCAGTGTGT
TCCATTTCTCTATATCTGGGATGAAAGCAGCAGTCTCTGCTCCCCACACTCCTGCCTAAATGCCCATTGA
GCATAACAGGGCTGTGGACTGTCCCCTACTGCCTATGGGGGACAGACACTTATTTACAATAGGTTGCTGG
CTAGGTGCCCTCATTTCTCACAGGAAGGACTCTTGGTAGCAGGGTCTGCCTTGTGCCAGGTGCTCACCAA
TGATTGCCCCGTGGTGACCTTTCCTTCTGCCCTTCCAAGGGCTTTATGACGGAAGAATGACTGTTCCATC
ATTTTGCAGATGGAAGGGGCCCTGGTGCCCTAACAGCGAATGTGAGAGCCCAGACACATGGCCCCTCTCT
CCTTTTCCCTCTCTCTGTCTCTCTGGTTCCTAAACCTGCCTTTAAGGCTCGAAGTTATGCTCCCCATTTA
TATTAAACATCTACTATGTGTTAGAGGTAAGAAGCAGTCAACAAAACAGGTGAAAATGTTGTCAATATCA
CGATAGATCACAGAGCACACCTGTATCGCCAGCACTGGGGAGGTGGAGGCCCAGGGGTCAAGAGTTCAGA
TTGTCCCTGGGTTTCATAGTCAGTCCAAGGCTGGACTGGGCTATATGAGATCCTATTTCGAAAGTACAAA
AGCAAAAGACCTACCTAAACGTAGCTTACATTCTGGCAGAGTTGATCAAATCAAGCACATAGGGTGCGCA
CTGCTTTGGGTAAGACGCAGAACAGCAGCTCAGAGCTGGGGTGTTTGTGGTTTAATGAGGTTTGGGGAAG
GGGTCCCCGGGAGGTGACATTTGAGAAAAATACCTGAAGAAGGAAGAAGCTTTGTGCACACTGGAGACAG
AGGTCCAGACTATTCTGTGGGGCAGCAACATGTTCAAAGGTCCTGGGGTGTGGACTATTCTGAAACCTGT
GATGACCAGCAAGGGTATCATGGGTACTTCCCAAAATGTGTTCAGAGCTTGCCAGATGTGTTCATGGATG
TTAGACATCATTAACAATGGGCTGTAAGGGCAGAGGCAGTGCACACCTGTTAGTCCTAAGGCTTTGGGGA
AGCTGAGGCAGGAGAGTTGTAAGTTCAAGTCAGTATGGGCTACCTTAGGCTGCAAGAATGCAGAGGACAT
ACCTGTAACATCAGCTCCAGGGGATCTGATCCCTCATATACAGGTACATGTATACACACACACACACACA
CACACACACTTGAAAATAAAACTGTAGCCAGACATGGAGGCATGTGCACACCTTTAACTCTAGCACTCAG
AGGCAGGAAATAAAATCCTTTCTTTTTTATTTATTTTAATTTAAAAAGAGAAAATTAGTTCCAGGGGTAT
AGCTTAGGCCTTGCCTAGCCTGGTATATAAAAGGCCCCAGGTTTGATTTCTAGTACCAAGGGAAAAAAAG
ATTCCAAGGCCAGACATTTGGTACACACGTGCGCACCCACAAAGAGCAACAGGTGTCTGCTGTAAGATTT
CTCTCGGAGCCCTCAGCCTGCTGCTGATTTCAAGGCCTGAAGAATGGGAGGCACACACTGCCTGCCCTGC
ACATCCTGATCTCAAAGCTCACATCCGATGTGGGATGAGCACTAGACAGGTAGTGGGACCGACATCCATC
CATGAAGTCTGGGAAGAGGCTGAGGTGGGCCACACATGGGCAGAGGAGAAACCAAGCATGGGTGTAATGG
CAGCAATCCAGACAAGGGGGTAGACACAGGGCTAGCGAGCGCCTGTGTCACCAGGAAGCTGTGGCAGCCC
TAGGGTTTTGTCCAAGCCTAAACATGATAACTGAGAGGTGGCTGGGCCAGCAGGGTGACACTGTCACCTT
TGTTTCCCCCTCACAGAGCCAGAAACGTTATCATGAAGATATCTTCGGTGCAGTCTTTTCCTATGGGGCA
AAAAAGGGCAGCGCCTTGGAGGAGCCCAAAGCCACGAGGCTCTGACAGCCCAGAGTTCCAGCTTCTGGCA
CTGAGTAAAGATAATGGTGAGGGGCTTGGGGAGACAGCGAAATGCAATCCCCCCCAAGCCCCTCATGTCA
TTCCCCCCTCCTCCACCCTACCAAGTAGTATTGTACTATTGTGGACTACTAAATCTCTCTCCTCTCCTCC
CTCCCCTCTCTCCCTTTCCTCCCTTCTTCTCCACTCCCCAGCTCCCTCCTTCTCCTTCTCCTCCTTTGCC
TCTCACTCTTCCTTGGAGCTGAGAGCAGAGAAAAACTCAACCTCCTGACTGAAGCACTTTGGGTGACCAC
CAGGAGGCACCATGCCGCCGCTCTAATACTTAGCTGCACTATGTACAGATATTTATACTTCATATT

Protein Sequence : Show Sequence

>gi|6754872|ref|NP_035057.1| nitric oxide synthase, inducible [Mus musculus]
MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLH
VTSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEF
INQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARN
CSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEF
TQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANML
LEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSF
QKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPW
KTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFN
TKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCA
FAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEP
QQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIF
PGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKL
ARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDH
TPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGI
APFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYV
QDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHED
IFGAVFSYGAKKGSALEEPKATRL

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis fadD26 mutant vaccine , Mycobacterium tuberculosis sigE mutant vaccine

34. PCDHB1

Gene Name : PCDHB1
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 29930
NCBI Protein GI : 14195607
Genbank Accession : AC010223
Protein Accession : NP_037472
Taxonomy ID : 9606
Chromosome No : 5
Gene Starting Position : 19530
Gene Ending Position : 22063
Gene Strand (Orientation) : +
Protein Name : protocadherin beta 1
Protein pI : 5.03
Protein Weight : 82950.69
Protein Length : 818
Protein Note : Also known as PCDH-BETA1; MGC138301; MGC138303

DNA Sequence : Show Sequence

>gi|28558729:19530-22063 Homo sapiens protocadherin beta cluster (PCDHB@) on chromosome 5
CAGTAACCTGTTGCAGAAAAGTGAAAGTATATCCGCAACAGTTGGCTCTGATTGCAGAGAGCGCGCTTGT
GAGAACTGATGGCGGGTACGCGCAGAAAATCTTTGCAAAACAGGCAAGTGGGATCTCTTCTCATTTTTCT
GTGCATATCTGTGGGGGATGCGACAACTATCCGCTATTCAGTGGCAGAGGAAATGGAGAGCGGCTCGTTT
GTGGCCAACGTAGCTAAGGACCTAGGACTGGAGGTAGGGAAGCTGGCTGCGCGCGGGGCGCGGCTGGTTT
CCGAGGGCAACAAAATGCATTTCCGGCTCCACCGCAAGACGGGAGATTTGTTTGTGAAGGAGAAACTGGA
TCGGGAGTCACTTTGTGGCAAAGCCGACCCTTGTGTTCTGCACTTTGAAGTAGTCCTGGTGGAGCCGCTG
CAGTCCTTCCGGGCCGAGGTCAGGGTATTTGATATCAATGACAATGCCCCAGTTTTCCTAAACAAGGAGC
CGCTTTTAAAGATTCCGGAGAGCACCCCTTTGGGTTCACGTTTTCCTCTGCAGAGCGCCCAGGATCTGGA
CGTGGGCCTTAACGGTCTCCAGAACTACACCCTGAGTGCCAATGGGTATTTCCACCTGCACACCCGCTTC
TGCAGCCACGGGCCTAAATATGCTGAGCTGGTGCTGAACAAACCCCTGGACCGAGAGGAGCAGCCTGAAG
TCAACTTGACAATTACGGCGGTGGACGGCGGGTCCCCGCCTAAGTCTGGCACAGCTCACATCCACGTGGT
GGTTCTGGATGTCAACGACCACGTGCCCCAGTTCTCGCGACTGGTGTACAGAGCCCAGGTATCAGAGAAC
AGCCCCAATGGCTCTTTGGTGGCCACGGTGACTGCCGTGGACCTAGACGAGGGCACCAACAAAGCGATAA
CTTACTCTTTAGCTCAAAACCCAGAAGCAATTCTCAAGACGTTTCAGATTGACCCTCAAAATGGAGAAGT
TCGACTAAGAGGACCCCTCGATTTTGAAGCCATTGAAACATACGACATTGACATTCAAGCTACAGATGGT
GGAGGCCTCTCTGCCCACAGCAAAGTCCTGGTAGAAGTGGTGGATGTGAATGACAATCCTCCCGAAGTGA
TGGTCTCCTCTGTGTCCAGCCCACTCCCTGAAGACTCACCACCACAGACAGTAGTAGCCCTTTTCACTAT
CAGAGACCGGGACATTCGAGTGGGAGGAAAAGTCACCTGCTTCCTCAGAGAAGACCTTCCCTTTGTAATC
AAACCTACATTTGGGAATTCTTACTCACTGGTCACTGACAGAAGCTTGGATCGGGAGGAGGTCTCAGGCT
ATAATATCACCATTGTTGCCATGGATACTGGACCACCTAGCTTGTCTGCCGAGACTATGATAGAGGTGCT
AATATCCGACGTTAATGACAATCCTCCAATATTTCGGGAAGATTCCTATATCTTGACTGTTCGAGAAAAC
AACAGTCCTGCGGTTTTTATTGGCAAAGTCCATGCTGAGGATCTTGATTTGGGTGAGAATGCCCAAATAA
CATATTCTCTGTTGCCTCCAAAAAACGGAGATCTTTCAGTCTTTGCTTACATATCCATAAATTCAGGCAA
TGGGAAGCTCTACGCGCTGAGAACCATGGATTATGAGGCCATTCAAGATTTTCAATTTGTGGTAAAGGCA
ACTGATGGGGGCTTCCTGTCACTGAGTAGCCAAGTTACTGTCAGAGTGGTTGTCCTAGATGACAATGACA
ATCGTCCAATGATCTTATACCCACTGCAGAACGGCACCTTGCCCTGCAATGACCTGGTGCCCAGGTCTGC
AGAGGCAGGCTACCTAGTGACCAAAGTGGTGGCTGTGGATGGTGACTCAGGTCAGAATTCTTGGCTTTCA
TATCATCTACTTAAGGCCACTGACCTTGGGTTATTTTCTGTTCAAAGACAAAATGGAGAAATCCATACAT
TAAGGCAGATATCTGAGAGAGACCCCATGATGCAGAAATTGATCATTCTTGTTCAGGATCACGGCCAACC
AGCTCTTTCCACTACTGTCTCACTCAACATCCTGCTGGTAGATGGCTTTTCAGAGCCCTACCTGCAGTTC
CAGGATCCAACCAAGCATTCTAGAAAGGTAAATCCATCCACTAAATATTTGGTCATTTCTCTGGTCATCC
TTTCCTTTCTCTTTCTCCTCTCTGTCATAGTGATCTTCATTATACATGTCTACCAAAAGATTAAATATAG
AGAAAAGTTCACAATTCAAGAGCATTTCTATGATGACTGTAATTTCTCTAACAACCTGGTACAAGGACAA
GGCAATGGATCCTTATCTCGGCCTTGTCCATATGAAATGTGTTCAGCCACTGGCACTGGTAATAGTGAGT
TTCGCTTTCTTAAGCGTTTTATGCCCAACTTCCCTTTCCCTCATGCCACTGGGGAGATAAAAATGGAGGC
TGGCTCCAGTTTGCCCCCAAATTCTGATAGGAATAAGTCTCAGAGATTAGAGGGCCATGACCAGGTATCT
GATGACTATATGTA

Protein Sequence : Show Sequence

>gi|14195607|ref|NP_037472.2| protocadherin beta-1 precursor [Homo sapiens]
MAGTRRKSLQNRQVGSLLIFLCISVGDATTIRYSVAEEMESGSFVANVAKDLGLEVGKLAARGARLVSEG
NKMHFRLHRKTGDLFVKEKLDRESLCGKADPCVLHFEVVLVEPLQSFRAEVRVFDINDNAPVFLNKEPLL
KIPESTPLGSRFPLQSAQDLDVGLNGLQNYTLSANGYFHLHTRFCSHGPKYAELVLNKPLDREEQPEVNL
TITAVDGGSPPKSGTAHIHVVVLDVNDHVPQFSRLVYRAQVSENSPNGSLVATVTAVDLDEGTNKAITYS
LAQNPEAILKTFQIDPQNGEVRLRGPLDFEAIETYDIDIQATDGGGLSAHSKVLVEVVDVNDNPPEVMVS
SVSSPLPEDSPPQTVVALFTIRDRDIRVGGKVTCFLREDLPFVIKPTFGNSYSLVTDRSLDREEVSGYNI
TIVAMDTGPPSLSAETMIEVLISDVNDNPPIFREDSYILTVRENNSPAVFIGKVHAEDLDLGENAQITYS
LLPPKNGDLSVFAYISINSGNGKLYALRTMDYEAIQDFQFVVKATDGGFLSLSSQVTVRVVVLDDNDNRP
MILYPLQNGTLPCNDLVPRSAEAGYLVTKVVAVDGDSGQNSWLSYHLLKATDLGLFSVQRQNGEIHTLRQ
ISERDPMMQKLIILVQDHGQPALSTTVSLNILLVDGFSEPYLQFQDPTKHSRKVNPSTKYLVISLVILSF
LFLLSVIVIFIIHVYQKIKYREKFTIQEHFYDDCNFSNNLVQGQGNGSLSRPCPYEMCSATGTGNSEFRF
LKRFMPNFPFPHATGEIKMEAGSSLPPNSDRNKSQRLEGHDQVSDDYM

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

35. PTCD1

Gene Name : PTCD1
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 26024
NCBI Protein GI : 38492358
Genbank Accession : AC004922
Protein Accession : NP_056360
Taxonomy ID : 9606
Chromosome No : 7
Gene Starting Position : 93650104
Gene Ending Position : 93673129
Gene Strand (Orientation) : -
Protein Name : pentatricopeptide repeat domain 1
Protein pI : 8.78
Protein Weight : 75381.12
Protein Length : 700
Protein Note : Also known as KIAA0632

DNA Sequence : Show Sequence

>gi|157734172:93650104-93673129 Homo sapiens chromosome 7, alternate assembly HuRef, whole genome shotgun sequence
TGAAACAGGGTCTTGCTCTGTTCCACAGGCTGGAGTGCAGTGGTGCAATGATGGCTCACTGCAGCCTCAA
CCTTCTGGGCTCAAGGGATCCTCTCACCTCAACCTCCTGAGTAGCTGTGACTACAGGTGTGCACCACCAC
ACCCAGCTAATTTTTAAATTTTTTGTGGAGATGGGCCCTTGGCCCAGGCTGGTTTCTTAACTCAAATGAT
CCTCTGGCGTTGGCTTCCAAAAGTGCTGGGATTACAGGTGTGAGGCACCTTGCCTGGCCTAAGTACTTTT
TTTTTTTGTTCCTCCTCCAAGGACTGTCCCGTTGCAATACTGAATGCCTTTTTTTTTTTGAGATGGAGTT
TCGCTGTCACCCAGGCTGGAGTCCAGTGGCACGACCTCAGGTCACTACAACCTCCGCCTCCTGGGTTCAA
GTGATTCTCCTGCCTCAGCCTCCTAAGTAGCTGGGATTACAGCTGCTACCACGCCCGAGTAATTTTTGTA
TTTTTAGTAGAGACGGGGTTTTACCATGTTGGCCGGGCTGGTCCTGAACTCCTGACCTCAGGTGATCCGC
CTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCGGCCTGAATGCTTTTTTTG
ATCAAGGGTGGGGAAGGTTTTTAGACCATGGCCTGATGCTCTTTCCCCATCTTTTTGACAGAACTCTATG
AATATTGTATTAAAGAAGGCTATGCAGACAAAAACCTGATTGCAAAATGGAAAAAGCAAGGATATGAGAA
CTTGTGCTGCCTGCGGTGCATTCAGACACGGGACACCAACTTCGGGACGAACTGCATCTGCCGCGTGCCC
AAAAGCAAGCTGGAAGTGGTAATGTCTGACACTCAAGCTTGGTGTTGTTTTCAGCTCAAAATTCTGCCTT
AGTCGACTGCAAGGGATCTTATGTTATTTGGTTGGGCTGGAATGTCGTTTTGTCCCTGGATGTCCTGCTG
GCTCTCCCTCGTGGGAGTGGGTCCCTGTATTCAGGAATCCATGTGAGGCAGCGTGTGGCTGTGTGTTTGT
TAGGTCTGGGGTCAATCTCAACTCCACTTTTGGGCAAATTACTGAACCCCTTTCCTCACTTAGGAAATGG
TGGTGGGGAGCCCTAATCCCAAGGTGGTGGCAGGGTGACATCAGGGAAGGACAACCAGTGAGTTCCTGTC
CCTTTCAGTCCTCACAGTGATACTTTAACATTACCATCACTATCTTTCCCGCCCCCCCAAGACGGAGTCT
TGCTTTGTCGCCCAGGCTGGAGTGCAGTGATGCCATCTTGGCTCACTGCAACCTCCACCTCCCGGGTTCA
AGCGGTTCTCCTGCCTCATCCTCCTGAGTAGCTGGGACTACAGGCATGCACCACCACGCCTGGCTAATTT
TGTATTTTTAGTAGAGACGGGGTTTCTCAATGTTGCTCAGGCTGGTCTCGAACTCCCGACCTCAGGTGAT
CCACCCGCCTCAGCCTCCCAAAGTGCCGGGATTACAGGCATGAGCTACTGCGCCCAGCCCACTGTGTTTC
TTCTTTCAGAACTTTCTGTTCCAAGTACCACTCCTAGGCCAGGCGCCTCAGGAGTGGTGCTGAGGGACAG
GAGCTGAGCTCCATCCGGTTCCAAGCCCCTCTGAAAGGCAGCTGTGGCCCGAGGCAGAGTCACAGCAGTG
GCCGCTCAGTGCAGCCCCTCAGCACAGTTGTTGGCCCCACCGCCCATCTGCCAGCCTGGCCTTCTTTTCC
CCAGCTCCACACAGGGGCCTCTTCACATGAGTCCCCTTTATGCTGCAGTGAGACTGACAATGGAGAGCTG
TGGCTGGATGCTGAGGACTTGGGTACTGAGCAGTGGGGGCGAGCAAGGGCCGCCTCCCCGACGGAAGACT
GGGGTGTTGGTTTTCTGATGGAGGCAGGGACAGGTGGCAGAGTGGCAGAAGCCCCCTTCCTCTGAGTGCC
CAGATTCCACAAGGAGTCCAGGTCAGGGGCTGGTGGGCTGGGGTGCTGCTTCAGCCCTGTGGCCAAAACA
GCCCGCTGTAGACTTACCTGGGTTTCACCAGAGGATCCTGTTTACTTAAACTATGGTCTTGTTCTTCCAA
AAAAAGAACTTGAAAAAGTAAAATAAACACAAGCCACTGTTCTAGGGCTTTCTGGCTGGCCAAGTGTTGG
GCCGCACCAGGCCTGGGGGACAAGACCCTTCTTTCTGGGGGGCTCTGGGCCAGAAGGGTGTGACTCAAGT
GCATAGTCTCCTGCCCTCTTCCCCCACCAGAGTGTACCAGCCCAGAGGCCCCCCTGAAGTCCCCAAACAG
TAGCAGAGCCACGTCTGCTCATCGCAGGGTCTGTCATGCTCACTTAGCAGAATAACGAGACTCATTCACA
CCGATTTCTTTTTCTTGATTGGCAAACGTGTGTTGGATTTGCAGAACATATTATAAATAGACATACAGAT
GTAACCTCGGTGTCAACAGCAGCTGGTTCTACCTTCATGAGGGAGCCAAATTTTCCCTGTCCAGAGCTGT
CAAGGAAGGGTTTCTGAGGTGTGTCCCTATATGGCATGGTGGCAGGTCCTTCGTGGGAGGGTTGCCACAT
ATGTGAGTGTGCAGGGGCGAGCGTGGCGCAGTGGCATCGTCTCACTGTCCTCCTCCGTTGCAGGGCCGCA
TCATCGAGTGCACACACTGTGGCTGTCGTGGCTGCTCTGGCTGAGGCTGGCGCGCTCCACCCTGGACTCT
GGACTTCGCAGGTTCCTGCCTGTCACGCCACCCCCTTCCTGGGAGCAGCGAGCAGTGCCCCAGGCCCGAG
TTGGAGCACGGTCTCTATGGGGAAGGCTTCGCTGTCTATCAGCTGTGATTTGTAAAAATAAAATCTTTAA
ATCTCTCGAGCCCCACGTCTCTTCTTTCAGAGCATCGGCCTATGGAACCCGGCGGGGCGGCCCAGGCCCC
AGGGACCAGATGCCCCAGCCCCCTTGTGGTGTGTGAGGTGACACACAAAGGTAGCTGGAGCTGGAAGTCC
CGTGAAGGTGACACGCAAAGGTGGCTGGAGCTGCACTTGGACCTGCTGGGAGCACAGGCACCTTGGGCCT
AGTGTGTGTCCTCACCAACACCTGTGACACGCTGCGGCTGTTCCTCAGGGCCTGGCTCTTCCCCCAGGCA
GGAGGTGACACCAGCTCACTTGTCCTGGGGCTCCCACAGAGCACTGGGGGCCGAGCACATTGTTCCAGCT
GTGCTCCCATCACCTGCCCCCAAGGGCACATCCGTCATCAGCCTCCTTGCCGGTGTCCTGGTCCCCCTGG
GGCTTGGTCCGGAACTTCTGCCAGGGGTGCGGGGTTTCCTCTGCGGGCATCACTGTCAGCCACTGCTTGT
AATAGGCTCGGAAGCCGTCAATCTTCTCCAGGTAGGTGTTCTTCCCTTGGTACTAGAATTAGAAAAGTGA
GGCTAGAATCACTCCTGTTACCTACAGCTAATCCCAAACCTCGGCAGCTGGCTCAGGCCTCAGGGAAGCT
GGTGGCTGCCATTTTCCCTTCCAGGTGGCTGCAGAGGACAGGGCAGAAAAGCTGTGAACACGCACTATGG
GACTAGCTTACTTCAGAGAAACAGGTCCCCGCTGGGTACCCTGGGGCAGGCCCAGAGCCATCTGCCCAAT
GGCGTTCTTCCCAAATAGGCACCACTGTGCTCCTCCATCCGCAGTTGCCACCCCAGGCCCCCAAGCCAGA
ATCACTGGGTCCCACCAGCTCTGCCTCCACAGCCTGCCGAGCCCCTCCCCATCAAAGCCACCTCCCTCTG
ACCATTATCTCAACACTGCCAAGATAGGCTCCATGAGATATGGGGAAAAAAGCAAAGACACACCAGACAC
AAACTGAGAGCTGGTAGGGGAATATACCAAAAAAGCCAAAAAGTGCTATCCAACATACAGCTACTACCAC
ACAATGACAAAAGGTTTGGCCGGGTGCGGTGGCTCACGTCTGTAATCCCAGCACTTTGAGAGGCCGAGGT
GGGCAGATCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAGCATGGTGAAACTCCATCTCTACTAAA
AATACTGGATGTGGCGGTGCATGCCTGTAGCCCCAGCTACCTGGGAGGTTAAGGCAGGAGAATGGCTTGA
ACCCGGGAGGCAGAGGTTGCAGTGAGCTGAGATCACGCCATTGCACTCCAGCCTGGGCGACAGAGCAAGA
CTCCATCTCAAAAAAAAACAAAAACAAAAAAAGGTTTGACTCAAAAGACTGGACAATTCTAAACCTTAAG
GTGCCGGATAGAGCCTGAAAGCACAGAAAGCAAAACTCAACAGAATCACGAATATAAACAAGTCCACCAT
CATGAGGGAATATGAGTACAGATGCTCCTGACAGCAGGGTTCCGCCCCCCAAAAACCCATCGTAAATCAA
AAATCAATCTGGCTGTGTGTGCTGGCTCACTCCTATAATCCCAGTGCTTTGGGAAGCCGAGGCAGGCGGA
TTACATGAGCTCAGGAGTTCCAGACCAGTTTGGGCAATATAGTGAAACCCTATCTAAAAGATTAGCTGGG
TACAGTGGTACATGCCTGTAGTCCCAGCTCCTCAGGTGGCTAAGGTGGGAGGACTTCTTAAGCCTGGGAG
GTCAAGGCTACAGTGAGCCATAATCGTGCCACTGCACTCCAGCCTGGGCAACAGAGTGATTCCCGTCTCT
TTAAAAAAAAAAAAAAAAAAGAAAAAAGAAAATGCGGCACGGCATGGGGGCTCACAACTATAATCCCAGC
ACTTTGGGAGGCTGGGGTGGGTGGATCACGAGGTCGGGAGTTCAAGACCAGCCTGGCCAAGATGGTGAAA
CCCCGTCTCTACTAAAAAAAAAAAAAAAACCACAAAAATTAGCCTGGTGTGGTGGCAGGCTCCTGTAATC
CCAGCAACTCGGGAGGCTGAGGCAGGAAAATCACTTGAACCTGGGCGGCAGAGGTTGCAGTGAGGCCAGA
TCGCATCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCATCTCATAAAAAACAAAAGGAAAATGCA
TCTATTGCTGGCAACACAGCAGAGGGTCCTGACTTTATAATTATGCAAAAGTGATATACATTCAGTAGAA
ACTAACCCCGCACAAAGTCGATCCTTACAGAGTTACATCCTGATAAAACCATTGCAAAGTTGAAAAATCC
TGTGTAGAATCATCTTTAAGTCAGGGACATCTGTCTGTGACTTAAATATGATATGCTTTAGGTCTATGAT
AGTCTTGTGACTGTCGAGTTGAACCCCTAGTGAGCCCTACGCCAGAGGGAAGAGACAATCCACTAGGAAG
ATATGCACAACATGTGATCAACAAAAAACCAGTACCCAGAATATATAAAAAACCCTGGAATCAATGAGAA
AAGGCTTGCAACCAACAGTTCAAAGCACAGACACAGTTCAAAAATGCCCAGCTATGCCTTCAGCCAGACA
CAATTTCCATTGCTGTCCCACCTATTTGTAGGAAGCCCTCAAAATGGCATTCAGGCGTGACACCTGCACG
GCGTTCCTGACCCCGCACCCTCTAGCTGTGTGTGTCCCTGCCCATTTACTTGTTTCCGCCCCATCCGGGC
TGTGCTTCAAGCCATAGTGGCTCTCGATGCTCCGGAGATGCTCAGCACAGAGTGTGATTCGATGGACCCA
AAAGCCAAACCCCAGAGCCTGATAGCCTCAGAACAGCAGGGAGAGGCTGTGGCCGTGACAAAGCAGATTC
CATGACTCTGCCCTGCTCCTGCTGATACAGGAGGTTGAAAGAAATTATTTAGGCAGATAATAAGGGCAAC
AGAGTCCTTGGCGAAATTTCCCTTTTAATAAAAAGCAGCCCCCAAATAATTTCTTTTCTAACAAAGAGCA
GCCTGAAAAATCGAGCTGCAGACATAGATAAGCAAACTGGAAGCTTGCAGGGGTAAATGCTGGCGGCTGT
GCCAACAGAAAAGGGCTACCTGGGAGCCAGATATGTTCAACGTGGGGGCCCCATCTTCCCTTCTTTGTTG
CCATGTGTACAGTAAAGAACCAGGCAACATGGCGATGGCCACCTGTCTGCATAATAAAAGGTTAGGGTGG
GGCACCAGCTTTTATACATGCTATGTAAATGGCACACCTGGTCCAACTAATCTTTTGCGTCCTCTGTAAA
TCAGACACCACCTCCCCAAGCTCATCTACAAAACCTCTGCATTACACTATGGAAGTGGCAACCCACTTCT
CTGGCACCTCTCTCTGCAAGGAGAGAGCTCTTCTCTTTTTTTTTTTTTCACCACCACACCGGGCGAGAGC
TCTTCTTTCTTTCGCCTATTAAACTTCAGCTCTTAACCTCTTTTTTTTTTTTTTGAGACCAAGTCTCCAT
CTATCGCCCAGGCTGGAGTGCAGTGGCGCGATCTTGGCTCACAACAAATCTCAGGTATTCACCCCAGACA
ATGACACCACTTCACTGCCTGCCCAGTCCCTGAGCAAGCGACAACCTGTTTGGAGTACCCAGCAACGTGC
AGGTGCTGGGGACCCAGATGAGCACAGCAGACAGCCTCTGCCTCCAGAAGCCTGATACAGGGAAGGCCAG
GGCTGCCCATGAGCACATGATCCAAGACTGTAGGACACGGCATAAAGGAACAGAGTGGGAAGGGGGAGGG
GAGACTTTCATCCCAGGTCAGAGCGGCAGCCAGCTGGGCATGTCTGAGGGCCACTTAAGCAGAGACCTCC
AGGAAAAGTGGGTGCCACCTGAGGCAGCGGGAACACCAATCCCGAAGCAGGCAAGAGGTTGGTGGCCTCT
GCGAACTGACAGAAGGCCACTGGGACAGACAGAGAAAGCAAACGCAAGGCTGAGACATGAGATGAGCCAC
GCAGACAGGGACTCGCAGGCTGTGGTCTACAGACTTGGGTACTGCTCCTAAGGGAAGCTGCTGGACAGTT
TCAAGCATGGCAACACAATCCCAAGGGCCAGAACCGGGGTTGGGTGGTGGGGGGAGGGGGTAGCAATGGT
GAAGGAGCTGATGAGCTTTTGAGCTTGGGGCACACACCCGGTCAAAGGTGGGAGGGTACTGGGCTGCAAA
CTCCAGCTGGCGGATGACCACTTCGTTCACCGGGACCCTGTTCTGCTTCATGTCCTTCAAGATGCTGATG
AGATAGGTGTAGTTCAGCTTCCTGATGGCCGCGTTGATGAGGGCACTGTAGATGTGAGTGTTGGGGGTCA
CCTGGGACTTCTAGAACACAGGGACATCGTAGAATCAAGATGATGGCAGCCATTGGGACCCAGCAGCTCC
CACCCTCTGTCAATCAAGCTGGCTCCCGGGACCAGCGGCCAGGGCCAGCAAGGCAAACTTGGCAGCAACA
ATGTGCTGAATATCCCTCTCCAGCAGGAAAGCCAAGCCCAGCCCCTCTGCTCAGCCCTAAAGAAGCTGCG
GAAAAGGACGCTCGTGCTGCTCTCAGACACCGACTGACTCAGGCAGAAAATGATGTGGGAGACACCACCT
CCTTGCTCCCTGGGGTGGGCTTCCTGTGACGCACGGTCACCAGGTGGTGGTTGGCCTAGAAAAGTGCAAG
CCTTGGCTGGGTGAGCCCGGTAGGGTCCAGGAAAACAGCCAGAGGATGATGGACCTGGGGACACCGACAA
CCCAGGACGCAGCCTCTGCTGTGGTCGGATGAAATCCACGCAGCACTTCCAATGGGGGTTAGGTGGGAGG
CACAGACCTCGGGGGCTTTGAAAACTGCCCACGGCCCCTCAGGAATTCTGAGAATCCCTGCCATACCTCT
AGAGTGTTGGGGCCGAGGCCTGTGCCCAGCCCTGTGTCAACCCACGGCCACCTTCTTCCGGATGGGAACA
TCCGTGTGTCTCTACGCCCAGCCCTGAAGCACTCACTGGGGTCTGCTTCCCCCAAAATGGGACCAAAGAC
CAGAAGGTCATTGACCCCACTCTCTTTGCCCCATGCACAGTTCATCCTCTCCAGGTGGGGGGTCTGCAGA
CCCCTCAAACTTTCCTCCTGAGCTGCAGAGTGCTCCTGAGCACCATGGGTGTCCTGCCCAGGCCCGAGTC
CACTCACCTTCATGTCTGTGAGAAGCTGTAGACCGTCCTTCGGCCTGTGGCACCCGATGGCCAGGTTGCA
GAATGTCTGCAGGTTGGGGACGAGGCCCCTCTTTGCCAGGACCGGCAACAGCGCCTTGGCCCCCTCCAGG
TCTCCCAGCTTGCTCTTCTTTCTCACCAGCGTGTTAAAGAATGTCAGGTCGGCCTCTACCTGGTGCTCAT
CCAGGAGGGCCAGCAGCAAGGACTCTGCAGGACTCCCGGACTCCACCACCTCGGCCAGTAGCGTGAGGGT
CCTGATGTCGGGCTGCTGCCTGTGCTCTGCCATCTTGCTCAGGAAGCCCTCCAGGCCCCCTATCAAGGCC
AGCCGGTCAGCTGGGGTGGTCACCGTTCCAAAGGAGACCACTGTAGGGGGAACGGCCCCGGGGGTCAGGA
GGTTGACTTCCAGCTCCACGGGAGGTGGCTTCAGGGCCACTGCGGTGAGGGCTGCTGTGTGGCTGGGCTC
TGCCTTAGTATCCACCTCTGGTTGGGCCTTGCCGGGCACTCTGGCTTCCGGAGGCTCTGGAGGCCCAAGT
GCCTGAGAAGGTTCCAGAAACAGCTGCCTCTCCAGGGCCTCCACATGCAGCATGGCTGACATGAGGTTGC
CTGCCTTGGCCTGGGCTGTCCTCCTTGGCCGCTGCCTGCTCACTGGGGGCTGAAGCACAGTCGCCTCCTC
CCTGGGCTTCAGAAGCAGCTCTGAGGCCACCTGGGGGTCCCCTAGGCCACAGTCCCGAGCTGCCACCAAC
AGCAGGTTGTAGCTGTCCCGGCTCGGCTGTAGCCCTAGACTCAGCATCAGCCGCCACACCTGCCACGGAA
GAGACAAACGTCAGCTGGGTGCTCCCATTCAGCAGCTGGGCGCAGGGCTCACGCCTGGAATCCCAGCACC
TTGGGAGTTTTAGATGGGAGGATCCCTTGAGATCAGGAGTTCGAGACAAGACCGGGCAACATAGCAAGAC
CCTGCCTCAAAAAAATACGCCAGGCGCGGTGGCTCACGCCTGTAATCCCAACACTTTAGGAAGCTAAGGC
AGGCAGACTCCTTGAGATCAGGAGTTCGAGACCAGCCCGACCAACATGGTGAAACCCCATCTCTACTAAA
AATACAAAAATTATTGGGTGTGGTATTGCATGCCCACAGTCCCAGCTACTCAGGAGGCTGAAGTGGGAGA
ATCACTTGAACCCAGAGATCGAGGTTGCAGTGAGCCGTGATCATGCCACAGCACTCCAGCCTGGGTGACA
GAGCGAGAGTGTCTCAAAACAACTGTAAAACATTTAAAAAATTGGCCGGGCCTCTCCCTCTCCCTCTCCC
TCTCCCCCTCCCTCTCCCCACGGTCTCCCTCTCCCTCTTTCCACAGTCTCCCTCTGATGCCCAGCTGAAG
CTGGACTGTAACTGCTGCCATCTCGGCTCACTGCAACCTCCCTGCCTGATTCTCCTGCCTCAGCCTGCCG
AGTACCTGCGATTGCAGGCGCGCGCCACCACGCCTGACTGGTTTTTGTATTATTTTGGTGGAGACGGGGT
TTCGCTGTGTTGGCCAGGCTGGTCTCCAGCTCCTAACCGCGAGTGATCCACCAACCTCGGCCTCCCGAGG
TGCCGGGATTGCAGACGGAGTCTCGTTCACTCAGTGCTCAATGGTGCCCAGGCTGGAGTGCAGTGGCGTG
ATCTCGGCTCGCTACAACCTCCACCTCCCAGCCGCCTGCCTTGGCCTCCCAAAGTGCCAAGACGGCCGCC
ACCCCGTCTGGGAAGTGAGGAGCGTCTCTGCCTGGCCGCCCATCGTCTGGGATGTGAGGAGCCCCTCTGC
CTGGCTGCCCAGTCTGGAAAGTGAGGAGCGTCTCTGCCCAGCCGCCATCCCATCTAGGAAGTGAGGAGCG
CCTCTTCCCGGCCGCCATCCCATCTAGGAAGTGAGGAGCATCTCTGCCCGGCCGCCCATCGTCTGAGATG
TGGGGAGCGCCTCTGCCCCGCCGCCCCGTCTGGGATGTGAGGAGCGCCTCTGCCCGGCCGCGACCCCGTC
TGGGAGGTGAGGAGCGTCTCTGCCCGGCCGCCCCGTCTGAGAAATGAGGAGACCCTCTGCCTGGCAACCG
CCCCATCTGAGAAGTGAGGAGCCCCTCCGCCTGGCAGCCGCCCCGTCTGAGAAGTGAGGAGCCCCTCCGC
CCGGGAGTCACCCTGTCTGGGAAGTGAGGAGCGTCTCCGCCCGGCAGCCACCCCGTCCGGGAGGGAGGTG
GGGGTCAGCCCCCGCCAGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGTCAGCCCCCCACCCGGCCAG
CCGCCCCGTCCGGGAGGGAGGTGGGGGTGGTCAGCCCCCTGCCCGGCCAGCCGCCCCGTCCGGGAGGTGA
GGGGCACCTCTGCCCGGCTGCCCCTACTGGGAAGTGAGGAGCCCCTCTGCCCGGCTAGCACCCCGTCCAG
GAGGGAGGTGGGGGGGTCAGCCCCCCGCCCGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGGTCAGCC
CCCCGCCCGGCCAGCCGCCCCGTCCGGGAGGTGAGGGGCGCCTCTGCCCGGCCGCCCCTACTGGGAAGTG
AGGAGCCCCTCTGCCAGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGGGGTCAGCCCCCCGCCCAGCC
AGCCGCCCCGTCCGGGAGGTGAGCGGCGCCTCTGCCCGGCCGCCCCTGCTGGGAAGTGAGGAGCCCCTCT
GCCCGGCCACCACCCCGTCTGGGAGGTGTGCCCAGCGGCTCATTGAGAACGGGCCATGATGACAGTGGCG
GTTTTGTGGAATAGAAAGGGGGGAAAGGTGGGGAAAAGATTGAGAAATCGGATGGTTGCCGTGTCTGTGT
AGAAAGAAGTAGACATGGGAGACTTTTCATTTTGTTCTGTACTAAGAAAAATTCTTCTGCCTTGGGATCC
TGTTGATCTGTGACCTTACCCCCAACCCTGTGCTCTCTGAAACATGTGCTGTGTCCACTCAGGGTTAAAT
GGATTAAGGGTGGTGCAAGATGTGCTTTGTTAAACAGATGCTTGAAGGCAGCATGCTCCTTAAGAGTCAT
CACCACTCCCTAATCTCAAGTACCCAGGGGCACAAACACTGCAGAAGGCCGCAGGGTCGTCTGCCTAGGA
AAACCAGAGACCTTTGTTCACTTGTTTATCTGCTGACCTTCCCTCCACTATTGTCCTATGACCCTGCCAA
ATCTCCCTCTGCAAGAAACACCCAAGAATGATCAATAAAAAAAAAAAAAAAATTGGCCGGGCGCGGTGGC
TCACGCCTGTAATACTAGCATTTTGGGAGAGCAAGGCGCCGGATCACGAGGTAGGAGAGCGAGACCATCC
AGGCTAACATGGTGAAACTCTGTCTCTACTAAAAATACAAAAACAAAATTAGCTGGGCATGGTGGTGGGC
GCCTATAGTCCCAGCTACTCAGGAGGCTGAGACGGGAGAATGGTGTGAACCCAGGAGGTGGAGCTTGCAG
TGAGCCGAGATCATGCCACTGTACTCCAGCCTGGGCGACAGAGCAAGACTCCATCTCAAAAAAAAAAAAA
AAAAATTAAAAACATTAAAAAGAAAAAGAAAAAAAAAGCTCTCCAGCGGGGAGCTGTGGTTCACACCTGT
AATCCCAGCACTTTGGGAGGCCCAGGCGGGCAGATCACATGAGGCCAGGAGTTCAAGACCAGCCTGGTCA
ACATGGTGAAACCCCGTCTCTACTAAAAATACAAAATTAGCTGGGCGTGGTGGCGGGCGCCTGTAATCTC
AACTACTCGGGAGGCTGAGGCAGGAGTATCGCGTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATC
GCTCACTCCAGCCTGGGCAAGAGTGAGACTCCATCTCAAAATAAATGAATGAATGAATGAATGAATGACT
CCTGGGTTGCACCTGCAGAAGTGCTAATGGGGGTGTGGGAGGGTTGAACAAGTCCCCAGGTGAGTGTGTG
GCAGGCTTTCAAGGAAGGTGAGTTTAGAGGGGCTAAGCCGACTGCAAAGCAGGGTGGGAGGGGCGGGGGC
CACCCTCATTCCATCTCCAAGGGCCTCCTCTCCCTGCCATCTGTAGAATGGAGGCTACAGTGGCTGCTGG
GTTTTCAGAAAAGAGCACAGATATAAAAATACTCAGCACAGGGCCATCGTGGGTGCTCACAGGATCACCA
TTTTGCACCTCTGGCACCGGGGAAGCAGGGCAGGAAGGTGGGGTGGGAGGGACATACCTGGAGGGCGTAC
CGGAAGCCTGTCTTCTTGTCTTGGATGCAGCCCATGAGCAGGAAACTGAAGGTCTCCTCTGTGACCACGT
GCCCTTTGTGGATGATTTCCTGGGGGAGGGAACAAAGACGTCCCACTGAGAACCTGCAGCAGGCTGGGCA
TGGTAGCTCATGTCTGTAATCCTGGCACATTGGGAGGCCAAGGCAGGATGATCACTTGAGGCCAGGAGTT
TTGTGACCAGCCTGGGCAACATAGCAAGACCCTGTCTCTAGGAAGCCTTCCCTCGAGTGCACAAAGCAGG
ACACAGCACACGGTGGCATACACAATCTCCAGTAAGGTCTCATCAGATCCATCTGTGAACCCCAGGGCCC
AATACCGGCTGTCTCATCCTCCCTAAGCCTTCCTACATGAGGAGAGACAGACACCACAGCCCTGCCCCTG
ACACGTGGGACCAGCCCCATGAAGGGGAGCAGGACGGCAGGGGAAGCCCCACCTTGAACACATCGAGGCA
CATCCTAAGGTCTGCGCACTTGGCAGCCATCTTCAGCAGCGCGTGGTATGTTTTCAAGTTGAGCTCGAAG
TTTTTGGCCTGCAGCTGCTGCCGGAGCTTCAGGGCGCTCTGTAGAGCTGAGTCCTTCCAGGGGGACTCGG
CACAGACGTTGAACAGGGCCGTGTAGGTGGCGTCCGAGGGCTCCAGGTCCCGCTTTTTCATCTGTGGAGA
TGGAAGAAGCTCAGTGGTGGCCCGGGATCAGCTGGACGCACAGGTGAGCAGCTGCCCCAGAGGAGGCTGA
GGCTGGAGAGGACCCCGTCACTGCATCCACCAGGCCAGGCTCTAAGGCAGAGCCATGGACACATCAGAGC
CCAGCTCTCCAAACTCAGACACATTGAAGGGACTCTGAAAACTGAGCCCACACACCCAGATGTACCAAAC
AGGAGCTACCCTGCCCTGGGCACTTGTCAGCCCACTCCAGGCCTGGGGTCCTGGCAGCAAGGTCTTCCTG
ATGTCCCCACCTTCAGGCCACCCCGTCAAGGGGAAAGGGCCTGTAGTGCTTACTACCTGAGGGCTCCCGG
GCAAGTTAGCACTTCCTCGCGAAAATGGGGATGACACCATCACCTTGAAACCAGGCATGCCTGTGGCAAG
TGGGAGGATGCACTGCAGTGCTCTAGGGGTGGCCCCGAACCAGTGGCACAATGCACATTCTCAGGGCCAC
CTGATCTGGTAAGTCACAAGCCCCGGAGCTGAGTGACACTCAGCTTACTGAGCCTCCAGGGGACTAAGGC
ATGTGCTCAGGGTTGACAATCCGTGGTGAAGCCTAGCACTCGCTATTGGCCAGAGAAGGGCCATCTGGAC
AAAGGCAGGCGATAGGCAGCTCAGCGGCAGAAAGAACCCTGCTAGAGTCAGGCCATCTGGGGCTCAAATC
CCAGCCCCGGTGCATTCAAGTGGGGTGGCCTTGGACATGTCCCTTCATCTCTCTGTGCCTCAGTTTCCTT
ATCTGAAAAGCAAAGAGGGCAGTCCCTACTTTGAGGATGATGTAAGCTCTGCGTGTATTGCTGCACTCCA
CAGAGGTGCACTGACAACTCACTACCTGCCCAGCAGCAGCCCTGGGGTTAGCAGAGCGGGCACAGCCTAT
CCTTACTGAGCTTACACTCAAGAAGGGAGACACACTGCAGGTGAGTCACCAAAAACAGTAACAAGGCTGG
GTGCCGTGGCTCACGCCTATAATCCCAGCACTTTGGAAGGCTGAGGTGGGCAGATCACCTGAGGTCAGGA
GTTCGAGACCAGCCTGGCCAACATGGCGAAACCCCGTCTTCTACAAAAAACACAAAAAAATTAGCCAGGC
ATGGTGGTGGGCGCCTTTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGG
TGGAGGTTGCATTGAGCCGAGGTCGCACCACTGCACTCCAGCCAGGGTGACAGAGCCAAAACTCCCGTCT
CAAAAAAATAAATAAATAAAATAAAATAAATAAAAACAATAAAAATATTAGTTTTTTTTCTTTTTTTTTG
AGATGGAGTCTTACTCTGTCGCCAAAAAAAAGGACTGTAGGAAAAAGAAAGAGAGATCAGACTGTTACTG
TGTCTATGTAGAAAGAAGTAGACACAAGAAACTCCATTTTGTTCTGTACTAAGAGAAATTCTTCTGCCTT
GAGATGCTGTTAAACTGTAACCCTAGCCCCAACCCTGTGCTTGCAGAGACATGAGCTGTGTTGACTCAAG
GTTTAATGGATTTAGGGCTGTGCAGGGTGTGCTTTGTTAAAAAAGTGCTTGAAGGCAGTAGGCTTGATAA
AAGTCATCGCCATTCTCCAATCTCGAGTACCCAGGGACACAATACACTGCGGAAGGCCGCAGGGACCTCT
GCCCAGGAAAGCCAGGTATTGTCCAAGGTTTCTCCCCATGTGATAGCCTGAGATATGGCCTCGTGGGAAG
GGAAAGACCTGACTGTCTCCCAGCCTGTCCCCCAGCCCGACGCCCATAAAGGGTCTGTGCTGAGGAGGGT
TAGTGAAAGAGGAAGGCCTCTTTGCAGTTGAGATAAGAGGAAGGCATCTGTCTCCTGCTCGTCCCTGGGA
ATGGAATGTCTTGGGTGTAAAACCAGATTGTGTGTTCTATTTACTGAGATAGGCGAAAACCGCCTCAGGG
CTGGAGGTGGGACCTGCTGGAGACAATACTGCTCTTTGATGNNNNNNNNNNNNNNNNNNNNAAATGGGGA
TGACACCATCACCTTGAAACCAGGCATGCCTGTGGCAAGTGGTAGGATGCACTGCAGTGCTCTAGGGGTG
GCCCCGAACCAGTGGCACAATGCACATTCTCAGGGCCACCTGATCTGGTAAGTCACAAGCCCCGGAGCTG
AGTGACACTCAGCTTACTGAGCCTCCAGGGGACTAAGGCATGTGCTCAGGGTTGACAATCCGTGGTGAAG
CCTAGCACTCGCTATTGGCCAGAGAAGGGCCATCTGGACAAAGGCAGGCGATAGGCAGCTCAGCGGCAGA
AAGAACCCTGCTAGAGTCAGGCCATCTGGGGCTCAAATCCCAGCCCCGGTGCATTCAAGTGGGGTGGCCT
TGGACATGTCCCTTCATCTCTCTGTGCCTCAGTTTCCTTATCTGAAAAGCAAAGAGGGCAGTCCCTACTT
TGAGGATGATGTAAGCTCTGCGTGTATTGCTGCACTCCACAGAGGTGCACTGACAACTCACTACCTGCCC
AGCAGCAGCCCTGGGGTTAGCAGAGCGGGCACAGCCTATCCTTACTGAGCTTACACTCAAGAAGGGAGAC
ACACTGCAGGTGAGTCACCAAAAACAGTAACAAGGCTGGGTGCCGTGGCTCACGCCTATAATCCCAGCAC
TTTGGAAGGCTGAGGTGGGCAGATCACCTGAGGTCAGGAGTTCGAGCCCAGCGTGGCCCACATGGCGAAA
CTCTGTCTACTAAAAATACAAACAAAATGAGCCAGGCATGGTGGCATGTGCGCCTGTAGTCCCAGCTACT
CGGGAGGCTGAGGTATGAGAACTGCTCGAGCCCAGGAGGCGGAGGTTCCAGTGAGCCGAGGTCGCACCAC
TGCACTCCAGCCTGGGCGACAGAGCGAGACTCTGTCTCCAAAAAAAAAGGACTGTAGGAAAAAGAAAGAG
AGATCAGACTGTTACTGTGTCTATGTAGAAAGAAGTAGACACAAGAAACTCCATTTTGTTCTGTACTAAG
AGAAATTCTTCTGCCTTGAGATGCTGTTAAACTGTAACCCTAGCCCCAACCCTGTGCTTGCAGAGACATG
AGCTGTGTTGACTCAAGGTTTAATGGATTTAGGGCTGTGCAGGGTGTGCTTTGTTAAAAAAGTGCTTGAA
GGCAGTAGGCTTGATAAAAGTCATCGCCATTCTCCAATCTCGAGTACCCAGGGACACAATACACTGCGGA
AGGCCGCAGGGACCTCTGCCCAGGAAAGCCAGGTATTGTCCAAGGTTTCTCCCCATGTGATAGCCTGAGA
TATGGCCTCGTGGGAAGGGAAAGACCTGACTGTCTCCCAGCCTGTCCCCCAGCCCGACGCCCATAAAGGG
TCTGTGCTGAGGAGGGTTAGTGAAAGAGGAAGGCCTCTTTGCAGTTGAGATAAGAGGAAGGCATCTGTCT
CCTGCTCGTCCCTGGGAATGGAATGTCTTGGGTGTAAAACCAGATTGTGTGTTCTATTTACTGAGATAGG
CGAAAACCGCCTCAGGGCTGGAGGTGGGACCTGCTGGAGACAATACTGCTCTTTGATGCACGAGATGTTG
TGTAGGTGCACATCAAAGCACAGCACCCTTCCTTAAACTTATTTATGACACAGAGACCTTTGCTCACATA
TTTTCCTGCTGACCCTCTCCCCATTACCCTATCGTCCTGCCACATCCCCCTCTCCGAGATGGTAGAGATA
GTGATCAATAAATACTGAGGGAACTCAAGAGACCAGTGCCTGCGCAGCGCAGGTCCTCCGTATGCGCAGG
TCCTCCGTATGCGGGATGCTGAGCGTATGCTGAGCACCGGTCCCAGGGGACCACTGTTCTTTCTCTATAC
TTTGTCTCTGTGTCTTATTTCTTTTCTCAGTCTCTCGTCCCACCTGACGAGAAACGCCCACAGGTGTGGA
GGGGCTGGCCCCCTTCAGAGGAGCAGATGCGGTGGCTGGGCTGTGACCTGTAATCCCAGCACTTTGGGAG
GCTGAGGCGGATGGCCAGGGGTTAGAGACCAGCCTAGACAAAGCAGCACAATCCCACCTCTACAAAAAAC
TTAAAAATTAGCCAGATATGGTGCTACACACCTGTGCTCACAGCTACTGGCGAGGCTGAGGCAGGAGGAA
CTCTTGAGCCAAGGAGTTGGAGGCTGCAGTGAGCTATGACTGCACTCCAGCCTAGGTGACAGAGTGAGGC
TCCCTCTGTTTTTTTTTGAAGGGAAAAGAAAGGGAACATGGGGACCGCCACAGTCCAGGCAGGTGCTAAA
GGGCTTTCCATGTCATCTCGTTTATCACCCACAATCACCACCATTTTAAGGAGATGACTCTGAGGCCAGG
GAGGTGAAGTCACCTGCCCAGTGTAAAGCACTAGCAAGTGGCACACTTGGGATCCGCCCCCAGCTTTTCA
GAGCCTCACCCCGGCTGCCCTGCGCCCACATGCACCTGGTTGTAGAGGTTGAAGGCCTTCTTCAGGTAGC
CAACCCGCCCGCAGCCCCCAATCAGCACCGTGTAGTTGCTCTCCATGGGCTGCAATCGCTCCTCCTTCAG
CATCTGCCTCTCAAACAGGTCCAGGGCTTCAACCAGCTGCAGGGAAGAGGCAACAGGGCAGGGGCTCAGA
ATGGCCCCAGAGACCCTCAGCAGAGAGAGGGAGGTTGAAGCTGAGGGACCCTCCTAAAATGGTGGAGAAC
GGCGGCCCTGGGTGACTGCAAACCCCCTGGGCCCCCAGCTGTTTCTGCCCCATCCCTCACCTCACACCAT
TTCCCCGGTGAAGCCTCAGTTCATTCCTTTATTCCTTCATTATTTTCATCTGTTCATTCAAACAATAATT
ACTGAGTGCTTCAATGAGCCAGACAAGGTCTATCCACTGCATATACGGCTGGGAACAAGAGAGAGCTCCT
TCTTGGAGCTCATGTTTTGGTGTGGAAAGTCAGACATGAGGCCAGGCGCAGTGGCTCACGCCTGTAATCC
CAGCACTTTGGGAGGCTGAGGCGGGCAGATCACAAGGTCAGGAATTCGAGACCAGCCTGGCCAACATGGT
GAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCACACACCTGTAATCCCAGCTAC
TTGGAAGGCTAAGGCAGAAGAATTGCTTGAACCCGGGAGGCAGAGGCTACGGTGAGCCAAGATTTCACCA
CTGCACTCCAGCCTGGGTGACGGTACAAGACTCCATCTCAAAAAAAAAAAAAAAACAAAAAAACAAACCA
GACAGACATGAAATGGGATAATTAAGAAAGACACACAGTATATCAAGTGATGACAGGTGGTACGAACAAA
ACTAAAGCAGCGGCCAGGTGTAATGGCTCATGTTTGTAGTCCCAGCATTTCGGGAGACCAAGGCGGGCGG
ATCACCTGAGGTCTGGAGTTTGAGACCAGCCTGGCCATCATGGAGAAACCCCATCTCTACTAAAAATACA
AAAATTGGCCAGGTGTGGTGGCACATGCCTGTAATCCCAGCTACTCAGGATGTTGAGATGGGAGAATTGC
TTGAATTCCCCAGGAGGCGGAGGCTGCAGTGAGCCAAGATCGTGCCACTGCACTCCAGCCTGGGCGACAG
AGTGAGATCCTGTTATAAAAAAAACCTAAAGCAGGGAGTGCTAGAAGGGTGTGGAGGATTCACTTTTCTT
TTTCCTTTTTTTTTTTTTGAGACAGACTCTTGTTCTGTGACTCAGGCTGGAGTCTAGTGGTAAGATCAGC
TCAGTGCAACCTCCGTCTCTACAAAAAAAAAAAAATACAATAAAATAAAACTGCGGTGGAGGATGGCAAA
GGGTTGCGATACTTACAGCCATAAGGCTGGCAGTTCAACAGGGTAGGCCCAAAACTTAGAGGCTGGGAAA
GTCAGGTGACCCCTTGCAAAACACTGAGCCACCAGGGGAAAGAAGCCAGGAGCCACAGAACCCAAAGTGC
CCCTTACCTTCCCTTCCTTGATCAGGTGTTTGCACTGCAAGAAGTACCAGTACGGGGTGTTTCTCCGGCC
TCGCCATAATTTGGGCTCCGGTTCCATTTGCTCATCTCTCCGTTCCCCAAACCGCAGGTTATGGAACTGG
GCTGCGGATTTGCGGAATAGTCTCCGGGAGGAGTATTTGTCAGAGAGGGTCCCAAAACTCTCCTCCTCCT
CCTCGTCTTCTTCCTGCGTGGCCGTGGAGTTGGAGTGGCTCGGGTCAGAGCCCAGGCTGCCCGTGTTTTC
CTGACGCTCCTGGCCGAGGGGCAGCTGAGAGGAGGAGCTGCTGAAGGGCGCCCACATTGGCCGCATCAGC
CCCTCCCTGCCTCCTGCCCACCTGGCTCTACAGGGGTCCAGGTGTTGCAGGATGAACAGTCCCATGGGGC
GGGCCCTGGCGAACAGTCGAGCGAGTCTCACGAAGTCCATTTCTGGCTTTCCTGTCCCTCCAGGGCCTGG
AATATATCAGGAAAAATAAGAGAGTCAACTCAGTTCCTAGTAACAAAAGGCAGAAAGAAAGAAGTGGTTA
CAAGTATGGGCCCAGGCCAGGCGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGTCGAGGCTGG
CATATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGACAACATGGTGAAACCCCGTCTCTATGAAAAAT
ACAAAAATTAGCCAGGCTTGGTGCTGTGCGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAAT
CACCTGAACCTGGGAGATGGAGCTTGCAGTGAGCTGAGATTGCGCCACCATACTCCAACCTGGGTGACAG
AGTGAGACTCTGTCTCGAAAAAAAAAAGAAAAGAAAGAAAGAAAAAGAACAGGCCAGGGGCGGTGGCTCA
TGCCTGCACTCCCAGCACTTTGGGAGGCCGAGGCGGGCAGATCAGTTCAAGACCAGCCTGGCGACACCCC
ATCTCTACTAAAAATACAAAAAACATGAGCTGGGTGTGGTGCTACACGTCTAATCCCAGCTACTGAGGAG
ACTGAGGTAGGAGAATCGCTTGAACCCAGGAGACGGAGCTTGCAGTGAGCTGAGATCGCGCCACTGCACT
CCAGCCTGAGTGACAGTGAGACTCTGTCTCAGAAAAAAAAAAAAATAATAATAATAAATAAAATAAGAAG
TATGGGCCCAGAGGTTAGAAAGCCTAGGTTCAGGTCAGACATAGCTCACTGCAGCCCCACCCTCCTGTGC
TCAAGCGATCCTCCCATTTCAGCCTCCCGAGTAGCTGGAACTACAGGTGTGAGCCACTATGCCTGGCTAA
TTTTATTTTTTGGTAGAGACAGGGTCTTGCTATGTGCCCAGGCTGGTCTTGAACTCCTGGACTCAAGCAA
TCCTCTCGCCTCGACCTCCCAAAGCACTGGGATTACAGCAATGAGCCACCATGCCCAGCCTAAAGTATTT
TAGAGTCTGGTTATATTAGAAGGTTGCGATTGGAAAAGTCTTACAAGTGATGGCAGATTGAACAGATTAA
AAACTAGGCCCAGTAGGTCAGGAGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGG
GTGGATCCCCTCAGGTCAGGAGTTCGAGACCAGCCTGACGTGGTGAACCCTATCTCTGCCGAAAATATAA
AAATTAGCTGGGCGTGACAGCGTGCCCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACT
TGAACGTGGGAGGCAGAGGTTGCAGTGAGCCAAGATCGCGCCATTGCACTCCAGCCTGAGCGACAGAGCA
AGACTCCATCTCAAAACAACAACAACAACAACAAACAAAAAACTAGGCCCAGCAAGGGGAGAGACTTGCT
GGGGTCCTGCAGCAGTGCAGTGGGTGAGACTGGCCAGCTTGGGCTGGGCTGGCTTTGAACTCAGGTCCAT
GTAACGCATTGATGATTGTCCTGACCAGCACCTTTTGTGAAACTAGTGCAGGGGGCCTTTATCATTCTGC
ATCTGGCAAGCCCTGCCACTCCTTCTTACAAGCATGGTGTTTATGTAATTTTCTTTTTTGTGGAATGGGG
TCTTCATGTTGCCCAGGCTGGAGTGCGATGGCACAGTGGAGTGCAAATGCACAATCACTGCAACCTCCAA
CTCCTGGGCTCAAGTGATCCTCCTACCTCAGCCTCTCAATTAACTAGCACTACTGGTGCACACCACCATA
CCTGTCTAATTTTTTGTATTTTTTTGTAGAGATGGAGTCTCCCTATGTTGCCAGGCTGGTCTAGAACTCC
TGGCCTCAAGCAATCCTCCAGCCTTGGCCTCCCAAAGTGTTGGGATTGCAGGCGTAAGCCTCCATGCCCT
GCCTTACGTGCATTAATTACAATACACAGAATTACAAAACAAAAAGCAATGATATTGAAATGGCATCAAA
ATGCTTTAAAAACAAATTTCTGCTATACTAGTATAGATGCTTATTTTCAATGCATGAAATAACAAGACCC
TATCATAATTTCTTTTCTTTTCTTTTTTTTTTTTTGAGACAGTCTCTCACTCTGTCCTCAGGCGTGACCT
CGGCTCACTGCAATTTCCGCCTCCCGGATTCAAGCGATTCTCCTGCCTCAGCCTCCCCTGGAGCTGGGAC
TACAGACGCATGCCACCAAGCCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTGGC
CAGGATGGTCTGGATCTTTTGACCTTGTGATCCGCCCGCCTCGGCCTCCCGAAGTGCTGGGATTACAGCC
GTGAGCCACCGCGCCCGGCCGACTCTATCATAATTTCAAAGCAGTAATAAATGTAAGTGACAGCTTAAGA
TACCACCTTGTAGTTGCTGCACAAACTGGGTTTTTTTGCGTCTCACTCTGTCACCAGGCTGGAGTGGGGT
GGTACAATCTCGGCTCAATGCAACCTCTGCCTCCCGGGTTTAAGCCATGCTCATGCCTCAGCCTCCCAAG
TAGCTGGGATTACAGGAACGCGCCACTACTCCAGGCCAATTTTTATATTTTCAGTAGGGACGGGGTTTTG
CCATCCTGTCCAGGCTGGTCTCGAACTCCTGACCTCACGTGATCCTCTCGCCTCAGACTCCCAAAGTGCT
GAGGTTACAGGTGTGAGCCACCATGCCTGGCCCTGCACAAACTGTTATCTGGAACTATCATTTACACTCA
CCCTCACAGTTTCAGGCACTGCTAAGGCTGCTCCAGCAATCAGGAAAGGAAATGCTATACTTCACATAGA
GAAAAATAGGGGTGCATTTTGTTCCTACACAAGCTCATTACCCCACCCTGGTTAAAAAAAACCCTGAATT
AGGCTGTATAGTGGCTCATGCCTGCAATTCCAGCACTTTGGGAGGCCCAGGCAGGAGGATCGCTTTGGGC
TTGGGGCCAGGAGTTCGAGACCAGCCTGGGCAACATAGCAAGACCCGCCACCCGCCCCCCCAACCCCGCC
GTCTCTACAAAAATTAAAAATAAAATTTTAAAAATTAGCCAAGTGTGGTGGTGCAGCGTCTGTAGTTCCA
GCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGTCTGAGAAGTTGACTGCAGTGAGCTATGATCGTA
CCCCTGTACTCCAGCCTGGGCCACAGAGCGAGAAAAAACCCTGAATTAGAGACACGCTGCGGCCGTCCTT
CCTCGGAGAGACCCGCCCCTCCTCAGAGGCCCACCCGGACCCTCAGGCCAACGCCGACCCCTCTCCACAC
GGACTTCTGCAGCCTCAGTTTCCCTCCTCAGACGCCCCCGGCTCCAATCCCGGCTCGGGCACTTCCTTCC
CCTCTGGCGTGTCTTGGCCCGGGTCCCGGGGCTCCTGCGAGGATCACACAGGAACTCACTTGAAGTGTCC
GGCGCAGTGCACTCCGACGGGGAGCCCTGCCCGGTCCCCGCGGCGAACCAGTCTCTTCCTCGGGTCCCCC
TCTCCCCAAGCGCGCAGGCGCAATCCGCGTCGCCGACTCACGCGGCTGGTTTCAGGTTTCTGGCGA

Protein Sequence : Show Sequence

>gi|38492358|ref|NP_056360.2| pentatricopeptide repeat-containing protein 1 [Homo sapiens]
MDFVRLARLFARARPMGLFILQHLDPCRARWAGGREGLMRPMWAPFSSSSSQLPLGQERQENTGSLGSDP
SHSNSTATQEEDEEEEESFGTLSDKYSSRRLFRKSAAQFHNLRFGERRDEQMEPEPKLWRGRRNTPYWYF
LQCKHLIKEGKLVEALDLFERQMLKEERLQPMESNYTVLIGGCGRVGYLKKAFNLYNQMKKRDLEPSDAT
YTALFNVCAESPWKDSALQSALKLRQQLQAKNFELNLKTYHALLKMAAKCADLRMCLDVFKEIIHKGHVV
TEETFSFLLMGCIQDKKTGFRYALQVWRLMLSLGLQPSRDSYNLLLVAARDCGLGDPQVASELLLKPREE
ATVLQPPVSRQRPRRTAQAKAGNLMSAMLHVEALERQLFLEPSQALGPPEPPEARVPGKAQPEVDTKAEP
SHTAALTAVALKPPPVELEVNLLTPGAVPPTVVSFGTVTTPADRLALIGGLEGFLSKMAEHRQQPDIRTL
TLLAEVVESGSPAESLLLALLDEHQVEADLTFFNTLVRKKSKLGDLEGAKALLPVLAKRGLVPNLQTFCN
LAIGCHRPKDGLQLLTDMKKSQVTPNTHIYSALINAAIRKLNYTYLISILKDMKQNRVPVNEVVIRQLEF
AAQYPPTFDRYQGKNTYLEKIDGFRAYYKQWLTVMPAEETPHPWQKFRTKPQGDQDTGKEADDGCALGGR

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

36. SLC11A1

Gene Name : SLC11A1
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 6556
NCBI Protein GI : 109255241
Genbank Accession : AC021016
Protein Accession : NP_000569
Taxonomy ID : 9606
Chromosome No : 2
Gene Starting Position : 219246751
Gene Ending Position : 219261616
Gene Strand (Orientation) : +
Protein Name : solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
Protein pI : 7.72
Protein Weight : 56200.3
Protein Length : 550
Protein Note : Also known as LSH; NRAMP; NRAMP1

DNA Sequence : Show Sequence

>gi|224589811:219246751-219261616 Homo sapiens chromosome 2, GRCh37.p5 Primary Assembly
CACAGAACACGGGGTGCCTGGAAGGGGAACAGATGTGTTGTGGGGCACAGGGCAGGCTGGGAGGGGAACA
AAGGTCCACTCCATGGGTAACCAGACCCTTCCGCCAGGGCTGGCCACTTCTGCCTTTGGAAAATGTTTCA
CAACGCCCCATGTTGTGTGTGTGTGTGAATCGGCCGATGTGAACCGAATGTTGATGTAAGAGGCAGGGCA
CTCGGCTGCGGATGGGTAACAGGGCGTGGGCTGGCACACTTACTTGCACCAGTGCCCAGAGAGGGGGTGC
AGGCTGAGGAGCTGCCCAGAGCACCGCTCACACTCCCAGAGTACCTGAAGTCGGCATTTCAATGACAGGT
GAGTAGTGGCCCCTAGGGACAGAGCCTGATTGGGGGGTGGAGTGGAGGAGATCACTAGGCTGGTGGAGAC
TTGAGGAAGCAAGAAAGCCCTTGGTCCCCTGTGGAAGCCTCTGGTCCCCCGTGGGAGTCCTAGTCTAGAA
CACAGTTCTGGACACCAGACTCAATCTCACTGGGTGGAGTCCTTCACACCCCTAAGCGGTCTACCTTTCC
TTTGTCTGAAGTCCGTGGATCAAAGCCCCTGGAGCTTTCTGGGCTGCCGGAGAAGGTGAGGGGTCCAGCC
AAGGGGCAGACATGTGGCCTCAGCTTGCCCTTCTTAGAGCCTAGGGTCTCTCTGCATTCTCTGTGGCAGG
AGATCTGCCTCTGGGCATCTGTCCTCACATGAGAGGACAGTAATTCTGAAGCTGCCATGTCCCACACATC
TGGAATGTTTGTTTGAGAGGTGGAGGTGATGGACCCAAGATGGGGGCCAGGGACCTTAGTTTCTCCACTT
TTCCGGGAGGAGGAGGGAAAGGATCAGGCGGCTTTAACTCTGGGCCACCAGAAAGAGGCAGGACACTCAC
CATGCTTCATGGGCCCCCACAGGTGACAAGGGTCCCCAAAGGCTAAGCGGGTCCAGCTATGGTTCCATCT
CCAGCCCGACCAGCCCGACCAGCCCAGGGCCACAGCAAGCACCTCCCAGAGAGACCTACCTGAGTGAGAA
GATCCCCATCCCAGACACAAAACCGGTGGGATGCTGGAAACTTCCTGGGGGCTTGCAAGATTGACAGGAT
CCTGAAGGATCCCATGGGCTGGAGAATGGGGTCTCCTTATGATCATGGGTGGCAAGTCCCTTCCAGGTCT
GAGCAGCAGTGGTGGACAGTGGCAACTGCCCCTTCCTGCTTCCGTCCCCAGTGCTTAGCTGGGGTGCTTC
AGTATTTGTCTAAAAGCGATTAAATGACTTTCTCAATGAATCTCAGCTTTCCTATGAAGGAAAAGAGGCC
CAGGGGGAATTGGGATTGGAGCCTTGAGGACATGTTATAGCATTTAGGAAAGGGCATAGGGAGAAGTGAA
GGAGGCTGCAGGCTGGCATCTAGCATCTAGGATGCTGTCACCTGCATTTCCAGTATCTTCTCTTTTTTTG
GAGATGGAGTCTCGCTCTGTAGCCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAACCTCCGC
TTCCCAAGTTTAAGCGATTCTCCTGCCCCGGCCTCCCGAGTAGCTGGGATTACAGGCATGCACCACCATG
CCAGGCTAATTTTTGTATTTTTACTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCT
TAGCTCAAGTGATCCACCCACCTCGGCCTTCCAAAGTGCTGGGATTACAGACACGAGCCAACGCTCCCAG
CCTCCAATATCTTCAGCAGATTTCTAAGTCAATATGCCATGCTCTGGAACAGGGTGGGGGGACCATAGCC
TCAGCGGACCACAGAGAGGAGGCCCCGTGGTCTGAAAAGGAGCTGGCAAGGGAGGAGCTGGGCTAGGGAA
GCTTGGCCCCCTCACCCCCTTAGACAGCCCCCTGTGAGGTCTCTGAGGACCTGACAAGTCAGGGCTTGAG
GGGCAGGCAGTTCAGGTGCAAGCCGGCTGCTGAGCTTAACAGATCCAGAAAAAGATCCTCTGTGACTCAT
CAGAGCATGCTGCTTCCTCCACCCATGCCCTCCCCATCCCTTGGGTGGCAGACCCAGGAATGGGCCATGG
AGGGCAGGGCTGGGCTGATGAGCCTGTTGGGGCTCCTCTAGGGGATGGCCAAGGCCAGCTGCCACCATCC
CTATACCCAGGACCCCCTCACTCTACTCCTCCCACCCCCCAACAGGGCACCTTCAGCCTGCGGAAGCTAT
GGGCCTTCACGGGGCCTGGCTTCCTCATGAGCATTGCTTTCCTGGACCCAGGAAACATCGAGTCAGATCT
TCAGGCTGGCGCCGTGGCGGGATTCAAAGTAACTAAGTCGGGACCTGAGTGGGGACACTTTCGAGAGGGA
GGTGACCAGGCTAAGTGTTGAAGGCCCCTGCTGGCCATGTTTCTCCAATGTGGCCTGGGAGCTGGTGTTA
AGTTCAAATGGGCCCGAAAAGGGTCCCCAGGGCAGCCCTGCCAGAAGGTGGGGCATTTGTGTGGGGGGTA
GGGGACTGGACTCCTCTCTTTAAAATATATATATGTATATTTATTTATTTATTTATTTAATTTTATTTTT
TGAGACTCAGTCTCACTCGGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAACCTCTG
CCTCCCGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGTGCATGCCACCACGCCTGGCTAATTT
TTGTATTTTTAGTAGAGATGGGGTTTCACTGTGTGGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGA
TCCACCGCCTCGGCCTCCCAAAGTGCTGGGATAACAGGTGTGAGCCACCTTGCCTGGCGTAATTTATTTA
TTTATTTAGAGATGGGGGTCTCACTATGTTGCTCAGGCTGCTCTTGAACTCCTGGACTCAAGCAATCCTC
CCACCTTAGCCTTTTAAAGTGCTGGGATTACAGGGTGAGCTACCAGCGCCCAGCCAGGATGGGACTCCAT
CTCTCCCACCCCCTCCCCGAGGAGTATGCTTGGTTAGAGGGTACCACGAGCTCAGGGGCTTTCTGAGGCT
GGCCTCTCTGGCTGAAGGCCTCTCCCTGCCTCCTCACAGCTTCTCTGGGTGCTGCTCTGGGCCACCGTGT
TGGGCTTGCTCTGCCAGCGACTGGCTGCACGTCTGGGCGTGGTGACAGGCAAGGACTTGGGCGAGGTCTG
CCATCTCTACTACCCTAAGGTGAGCTTGGGGGGCCTGGACAGGGAGAACCACTGGCCCCAAACCCCAAAC
AGCCATTTTCAGCTTCCACGATCAAATAAATACATCATCTCACATGGGGCATCCCAAGTCTGTTTGTTTT
TGTTGTTGTTTGTTTGTTTTTCAGTCGGAGTCTCGCTCCGTCGCCCAGTCAGGAGTGCAATGGTGCGATG
TCAGCTCACTGCAACCTCTACCTCCCAGGTACAAGTGATTCTCCTGACTCAGCCTCTCAAGTAGCTGGGA
TTACAGGCGCCCACCACCATCATCGGCTAATTTTTGTATTTTTAGTAGAGCTGGGGTTTCACCATGTTGG
CCAGGCTGGTCTCAAACTCCTGACCTCAGATGATCCACCCACCTCAGTCTCCCAAAGTGCTGGGATTACA
GGCATGAGCCACTGCATCCGGCCCCAATTCCATTTGATAGATGGAAAGACTGAGGCTCAAACTGATAGCA
CATAGCCTAGAGGGACATGAGAGGCATGTCATTTCACAGAAGGGGTAATTAAAATTTGGGAAAGAGACTT
GCCCAAGACAAAAAATGGGGAGGCATCAGCTCAGGAATGGTCACTGGTTGGGGTGGACCCTGGGGACTTC
GTTGTTGTGAGTGTCCAGTTTGGAAAACAGAAATTACAGCACTGGACATTTCAAAGGTATTGAATGCAGG
GATTGAAAGGTTTGGGAGGGCTTGAGGGGCAAGGGAAGAGGGTGTTACAAAAAGATCAGAAAGCTGCTAC
ACCTCTGGCACAAGCCCCAGAGCCATCCTTTGCTGCTGAGCTCCTGGAAGCATTATTGATGCTGATGAAA
TGGCCACTAGTGCTGCCAGAGTCCGGGTTGCCTGGTGCCACTGCCAGAAATACAAGAAGCAGGGGGAGAA
AATGGTTTATCTCAGCCTGGGGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGT
GGATCACCTGAGGTCGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATA
CAAAACTAGCCGGGCATGGTGGCACATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCG
CTTGAACACGGGAGGCGGAGATTGTGGTGAGCCGAGATCATGCCATTGCACTCCAGCCTGGGCAACAAGA
GCAAAACTCTATTTCGAAAAAAAAAAAAAAAGTTTATCTCTGCCTTTCCATCTCCTGTATGCTCTTCCTA
CATAAGGTAGGAAGCCCATTGGCCAGACTGTCTAGTAAATGTAGTCTGAGACGACAGACAAATGTAGTCT
GAGACGACAGGCAAATAACCGGCCCACCCTTAATGAAGGATCATCTCCTCCCCATAGGTGCCCCGCACCG
TCCTCTGGCTGACCATCGAGCTAGCCATTGTGGGCTCCGACATGCAGGAAGTCATCGGCACGGCCATTGC
ATTCAATCTGCTCTCAGCTGGACGGTACCACCCCAGTGTACCCCAACTCTTCAGGCCAGGCAGAGAACAG
CTGCTGCTACTTCCCCCCCTAACCAGTCCCTCCCAGAGTCTATTTTATCCTGCTGTCCCCTCTGAAGCAG
GGCTGCTGCCCTGTTTTCCAGAAATGTAAAGTGACTTGTCTAAAGTCACACAGATGTGAGTCATGCAGGA
CTTTGGGACTGCAGCCCCAAACTCCCTGCTGCGCCGGGTGCCAGGTCTCTCCTCTAGCTCTGCCCTGCCT
CGACTGTTCTATGCCACACTCCCACTCCCCTTGCCCTAGCTGTCTGGGGGCGCTTAGGGTCCTGCTCCCA
GGAGGCCAGATTCCTGTCTCCAGCCCTGAGGCTCCGTAGGAGTTAGAGACCCCTGGACCAGGCTGGGCTG
ACCCGGGCCACTCTGGTTTCAGAATCCCACTCTGGGGTGGCGTCCTCATCACCATCGTGGACACCTTCTT
CTTCCTCTTCCTCGATAACTACGGTGGGTGCACACCCCACCTCATAGGGGAGTGGTGGTGGTGAGGGTGC
TGTACTGGGAGAAGGGCTCTGACATCGAACAGCCTGGGAGCGCACCTGAGCTCCCTCACTCTCCCTGGGT
GGCCTCTAGCGAGTTACTTGGACGTGCCTCTCTTCACCTGTAACATGGGGAAATAATAGCACAGACTTCA
GAGGGTGGTTGTGAAGACTGAGTGAGGTAACATATGTTAAGCACTTGGCACTGTGCCTAGAAGCGCTCGT
AGTATTAGCCATTACGAATTGTTGATGTCACAGATTTTTTTCGTTGGTTTGTTTAGTTTGTTTGTTCTGC
TCCGTAGGGCTGCGGAAGCTGGAAGCTTTTTTTGGACTCCTTATAACCATTATGGCCTTGACCTTTGGCT
ATGAGGTAGGAAGCCAGTGCTGCAACCCCACTGTGGACCTCCCAAGATCATTCCTCTCCCTTCCCTCTGG
CCGCGGGCTGCGGGGGGCTGGGGTGGGATGGAGGCTGAGAAATGGTGACCGCGGCGTGGTTGCGAGGGGC
GGGGCTTGTCCTGACCAGGCTCCTCCCTGCAGTATGTGGTGGCGCGTCCTGAGCAGGGAGCGCTTCTTCG
GGGCCTGTTCCTGCCCTCGTGCCCGGGCTGCGGCCACCCCGAGCTGCTGCAGGCGGTGGGCATTGTTGGC
GCCATCATCATGCCCCACAACATCTACCTGCACTCGGCCCTGGTCAAGGTGAGCAGAGGGGAGGGGAAAG
GAGACCCCCTCACTCAGTCGGAGCCATGCTGGCTCCGCCTCCAAGCCTGGAGCCCCTCCCCTCTGGCTCC
TTCCCTCAGGATTTGCGGAGCCTGTAATAATTGAGGGACAGGCGGGGCGCCATGGCTCACGCCTGTAATC
CCAGCACTTTGGGAGGCGGAGGCGGGCGGATCACGAGGTCAAGAGATCGACACCATCCTGGTCAACATGG
CGAAACCCCGTCTCTACTAAAAATACAAAAACTAGCTGGGCGTGGTGGCGCGCGCCTGTAGTCCCAGCGG
CTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGGTTGCGGTGAGCCGAGATCTTGCC
ACTGCACCACTCCAGCCTGGCGACAGAGCGAGACTCTGTCTCAAAAAAAAAAAAAAAAGAAAAGAAAAAG
TGTCTACTGTGCGCTGGGAGGAACAGACCGTCAAGGCCTCTGCCCTTCTAGAGTTTCCGTGCTAGTGGGG
TAGACGGATGACAAGCAAGTAAACCAACAAAGGAAAGAATTATAGGCCGGGCGCAGTGGCTCACGCCTGT
AATCCCAGCACTTCAGGAGGCCGAGGCGGGCGGGCGGATCACGAGGTCAGGAGTTTGAGACCTCAACAGC
CTGGCCAACATGGTGAAATCCCGTCTGTACTAAAAATACAGAAAATTAGCCGGAAGTGGTGGCGCGTGCC
TGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAAGCCAGTAGGCGGAAGTTGCAGTGA
GGGGAGATGGTGACATTGCACTCCAGCCTGAGTGACAAAGCAAGACTCCGTCTCAAAAAAAGAAAAAGAA
CAAGAACTATAGAGCTGGGCGAGGTGGCTCACGCCTGTAATCCCGGCACTTCAGGAGGCAGAGGCAGGTG
GATCGCTTGAGCCCAGGAGTTCGAGACCACCCTGGGCAACATGATAAAACCCTGTCTCTACCAACAAAAA
ATAAAAAATAAAATAAATTAGTTGGGTGTTGTGCACACCTGTAGTCCTAGTTACTCAGGAGGTTGAGGCT
TGAGCCCTGGAGGTCGAGGCTGCAGTGAGCTGAGATCCCACCACTGCACTCTCAGTGACACAGTGAGACC
CTGTCAAAATAATAATAATAATAATAATAATAATTGCGAAAGGGACTCTGAAGTAAACAAAACAGAGAAA
TATGATAGAGAATGACTGAAAGTTGGGGAAGTAGATCAGGGAAAAGATATTACAAGCGGAGGGAAAGCAA
GTGCAAAGGCCACTGGCAGAAGTGAACAAGGCTTGGGTCCCTAACAGCTTAACAGTGGCTCACACCTGTA
ATCTCAGCATTTTAAGAGGCCAAGACAGGAGAATGCTTGAGCCCAGGAGTTCAAGGCTAGCCTAGCAAGA
CCCTGTCTCTGCAGAAAATTTAAAAATTAGCGGGTTGTAGTGGTGTGTGTCTGTGGTCCCAGCTACTTAG
GAGGCTGGGGCAGGAGGATCACTTGGGCCCATGAGTTGGAGGCTGCAGTGAGCCATGCATTGCACCACGG
CACTCCAGTCTGGGTGACAGAACAAAACCTGTCTCTAAAAAATAAAATAAAGTAAGCTGGACACGTCTGA
GGATGGAACAAGGTGAGTGAAGGAGCGTGTCAGGACCTGAGGTAGCCAGGGACCTCAAAGGCCAGCCTTG
CTTCACCCACACAGTGGCTTACAGTGGTAAGGCCTCTGTGGCAAGAACAGAGATGTAGGAAACCATCGGC
TGACCTGAACCTGCCCCAGACTGCCACGCAGGGGACTTAAGAAGGTACTGGGCTTTGGGGAGAACATAGA
AGTGTGAGGGTGGGGGACACTGTGGTGGCTCTGAGGGACTTTGGCACTTCCCTCTCCCTTTGATCTTCGT
AGTCTCGAGAGATAGACCGGGCCCGCCGAGCAGACATCAGAGAAGCCAACATGTACTTCCTGATTGAGGC
CACCATCGCCCTGTCCGTCTCCTTTATCATCAACCTCTTTGTCATGGCTGTCTTTGGGCAGGCCTTCTAC
CAGAAAACCAACCAGGCTGCGGTGAGACACACTTTCCCCCGCACCTGAGGCCACACACGTACTCATGTCC
TGTAAGCCTTGCCGAGGACCCTAGGCAATGCAGCTGAGCCCTTCTGAGTCTCTGCCCTGATGATCTTCCC
TGTTGGCAGATATCATTCATTCAGCAAATAATCATTGAGCATTTGTTATATACCAAGCACATCCTAGACC
CTGGGGATACAGCAGTCAATGCTACAAAGACCCAGCTCTCTGCAGCTTAAATAGGGAGGGAGGTGAGCAG
ACAGGAATGAATACCCAAGGGAAGTACAGAGTGGGAAGAAATAAAGGGGGTGAAGAAGCATAGTTGGGGG
TATGGGGGTGGGGTCTGCATTAGCCCAGGTGGTCAGGAAAGGCTTCTTGGAAGAGATGACATTTGTGCTA
ATACTGGAAAAGGAACTAGCCAGCTCCTCCCTGACTCTCTGGGATCTGGGACCTGTGTCTGTCTCCCCTT
CTGAGTGGATCTCAGTCATGAGGCTCTAACAACTGCAGCCAGGGTTGGGGGCTTCCTGAATGCTGAGCCC
TGTGGGAGAAGGGGTGGCCAGGCCTCCAAGGACCTGGGCACTGCCACTGCCTTGCTCTGTGACCTTGGGA
GGTCACACTCCTCCCTGGGTCTCACTCTGTTCCTTGGCACAAGGAGGGAGATCAACATCAGGACTGCAAA
CCCTTGTCTTCAAGACCAGGCAGGCGCAGAGTTGTGGGAAGTGGACAGTGTGAAGAGGGCATGGCAGCCA
GCTGTCTCTGTGGTGATGCCAGCTCTTACGAACCTAAAAGAAGCTGGAAACTGGGTTCTTATGCCAGGCC
TTCTCAATTTTAAATGTTGGCAACAAATCCACATTAAAGAAAAAAAAAACCCGGCCGGGTGCGGTGGCTC
ACGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGATGGCTCGCTTGAGCCCGGGAGTTCAAGACCAA
CCTGGGCAACATGACAAGACCCCCATCGCTACAAAACGTAAAAGTAAAACAAAACAAAACCCAACTTATT
TTGTTAGCCAAACAAAATATGTCTGGCCAGATTCAGCCTTGGGCCACCAGTTTTGGAACCCTGGTCAGTG
CTAGGCAGTCCAGTTTCCCAAGGCTGAGCGTGCTCCTCACATCTTCCTTCTACTGCCCTGGTACCCACAG
TTCAACATCTGTGCCAACAGCAGCCTCCACGACTACGCCAAGATCTTCCCCATGAACAACGCCACCGTGG
CCGTGGACATTTACCAGGGGGTGAGCGCGGGTGGGTGGGGAGGGCGTGACCCAGAGAGGCTCCCCGCCTC
GGGCAGGGCCACCGGTCCTACCACACTCGTCCCTGCAGGGCGTGATCCTGGGCTGCCTGTTCGGCCCCGC
GGCCCTCTACATCTGGGCCATAGGTCTCCTGGCGGCTGGGCAGAGCTCCACCATGACGGGCACCTACGCG
GGACAGTTCGTGATGGAGGTAGGGCAGGGGGCGGGCCCAGGAGGGCAAGGGGTCCAAGGACAGCAGGCAC
ACTACTGGGGGCTAGAACTCCGAGCCTTGTGGGTCCTCTTTTCTTTTCTTTCTTTTCTTCCTTTTTTTTT
GTTTTTGTTTTTGTTGTTGTTGTTGAGACGGAGTCTCGCTCTTGTCGCCCAGGCTGGAGTGCAGTGGCGC
GATCTCGGCTCACTGCAACCTCCCCCTCCCAGGTTCAAGCTTCTCCTGCTTCAGCCTCCTCAGTAGCTGG
GATTTCAGGCACCTGCCACCAGGCCTGGCTAATTTTTGTACTTTTAGTAGAGACAGGGTTTCGCCATGTT
GGCCAGGCTAGTCTTGAACTCTTGACCTCAAGTGATCCGCCTGCCTTGGCCTCCCAAAGTGCTGGAATTA
CAGGTGTGAACCACCGCACCCGGCCAATTTTTGTATTTTTAGTAGAGACGCAGTTTTGCTGTGTTGGCCA
GGCTGGTCTCAAACTCTTGACCTCGTGATCCGCCCGCCTTGGACTCCTGAAGTGCTGGGATTATAGGCGT
GAGCCACTGCGTCCGGCCTTTTCTTTTCTTTCTTTCTTTCCTTTTTTTTGAGACGGCGTTTCGCTCTTAT
TGCCCAGGCTGGAGTGCAATGGCGCGATCTCCGGCTCACCGCAACCTCCACCTCCTGGGTTCAAGCAATT
CTCCTGCCTCAGCCTCCTGACCAGCTGGGATTACAGGCCACCACACCCAGCTAATTTTGTATTTTCAGTA
AAGACGGGGTTTCTCCATGTTGGTCAGGGTGGTCTCGAACTCCCAGCCTCAGGTGATCTGCCCTCCTCAG
CCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCTCTTTCTTTCTTTTTTGAGACAGGA
TTTCACTCTGTTGCTCAGGCTGGAGTGCAATGGCGCCATCACAGCTCGCTGCAGCCTCAACTTCAGCCTC
CCGAGTCATTGGGACTACAGGCTCATGCCACCACACCAGGCTAATTTTTAAAATTATTTGTAGAGATGTG
GTCTTCCTATGTTTCCCAGGCTGGTCTCGAACTCCTGGGCTCAAGTGATCTTCCCGCCTTGGCTTCCCAA
AGTGCTGGGATTACAGACGTGAGCCACCCCACTCAGCTTTGTTTCCTTCATGTAGCCCAGAGGGCGACAC
TATAAGCCCCATTTTACAGATGAGAAAACAGATCCTGACAGATGAGGTGACTTGCTGGAAGCTGCAGACC
CAGGACTAACAGAAGCCTTTTCCCCAAAACTGTTTAGTCTTCAGCAACCAGCTATGGGATGGGAGCTCCC
CATTTCTCCCCACCCATCCCCTCTTGCCACCTAGGGACAGAGCTGTCCCAGTTCAACAGTGGAAAAACAG
AGCATGCCCCCAGGGATAAATCGGTTGAGGGACTACAGAGGATCTCTCCTCTGGAATCCCCAGTCCTGTC
TACTCCTCACCAAGGAGTTCACCCCCACCCCAGGGCTTCCTGAGGCTGCGGTGGTCACGCTTCGCCCGTG
TCCTCCTCACCCGCTCCTGCGCCATCCTGCCCACCGTGCTCGTGGCTGTCTTCCGGGACCTGAGGGACTT
GTCGGGCCTCAATGATCTGCTCAACGTGCTGCAGAGCCTGCTGGTGAGATGCGCCAACCCCACCAGCCCT
GCCCACTGCTGAGCGAAACAGGACCTCCAGCAACCCCCACGCTGAGCCTTGCATGGGCCTGCCAGGCCCT
GTCCCAGGCACATCTTGCCTACAAGGCTTCCATTGTCCCCACAGCCACCCTGGGGGGCAGGAGGGTAGGA
ATCACCATTATCCTTCCCCCCTCCCACCCCCCAGATGGTCTCCCTCTGTCACCTAGGCTGGAGTGCAGTG
GTTCAATCATGGCTCACTGCAGCCTGCCCTCCCTGTCTCTAGCAATCTTCCCACCTCAGGCTCCCGAGTA
GCAGGGACTATAGGTGCATGCCATCACACCCAGCTAATTTTTTTTTTTTTTTTTTTGAGACGGAGTTTCA
CTCTTGTTACCCAGGCTAGAGTGCAGTGGTGAGATCTTGGCTCACTGCAACCTCTGCCTCCCAGGTTCAA
GCGATTCTTCTGCCTCAGCCTCCTAAGTAGCTGAGATTACAAGCGCCCACCATCACACCCAGCTAATTTT
TTGTATTTTTAGTAGAGACAGGGTTTCATCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGA
TCCACCCGCCTTGGCCTCCCAAAGTGCAAGGATTACAGGCATGAGCCACTGAGGCAGGTCTAATTTTTTT
TTTTTTTTAAGTAGAGACAAAAGCTCATTATGTTTGTTGCCCAGGCTCATTATTCCATTTTACAGATGGG
AAAACTGATGCACTCAGTTAAGTTACTAGTGGCAGGGCTTGGATTCAAACCGAAGCAACCTGGTGCCAGA
GCCCCCAAACACTTTCTCCTGCTTTGGAGCCCACAACCTGATAAGGCAGGAGACGGGGAAGTAGGCTCAG
TGTGGTTAGGGCAGTTGAGCTCACACCCACACAGAGGGTGTCAAGCCACGCCCATCTTGCCCCCACCCTT
GCCATATTCTGAGGCAGAATTCCTCAGCCTAGGCTCCTGCTGCTCTCCCCAGCTCCCGTTCGCCGTGCTG
CCCATCCTCACGTTCACCAGCATGCCCACCCTCATGCAGGAGTTTGCCAATGGCCTGTGAGTACCCCCTT
TCCCAAGTGCTGGATTGCATCACCACATTTCATCCTCACAGCCACCCAGAGGTACGAGCTACAATTCTCC
CCATTATCCCAATGAAGCCACAGAGGCTGAGAGAGGTTAAGGGACTTACCCAGGTTCACACAGCTAGCAA
GTTGAGGAGCCAAGACTGGAATCCAGGGAAGAGGGCTGTTTCTAGAGCCTGAGCGCCTCACTCTCGCCTC
AGACTCAGCTCTTGCCAAATCCCGCCAGTGTGTAGCCTGGAGGGCCCAGGAGGCAGGAGGTGGGGAGGGG
GTCTGTCTGCTCCAGGTCCCACAGACCAGAGAAGCGGCCTCAGTGTATCCCCACCCCCAGTGTGGGCGCT
GGGAGATGAAGAGGAGTTGATGCAGGTGGGCCAGCAGCAACCAGCCAGTCCTGAGCCTCTCTCGTGTCCC
CCAGGCTGAACAAGGTCGTCACCTCTTCCATCATGGTGCTAGTCTGCGCCATCAACCTCTACTTCGTGGT
CAGCTATCTGCCCAGCCTGCCCCACCCTGCCTACTTCGGCCTTGCAGCCTTGCTGGCCGCAGCCTACCTG
GGCCTCAGCACCTACCTGGTACAGTAGGGCCAGGGGATGCCTTGGGAATGGATGAGGGAAGGACAAGAGG
CAACCAATGGGGAGGGTTTGGGGGGACACAATGGGGCTTCCCCAGAGGTCTTGGCATCTCCCCAATTCAT
GGTTGCCCCTCCCCCAGGTCTGGACCTGTTGCCTTGCCCACGGAGCCACCTTTCTGGCCCACAGCTCCCA
CCACCACTTCCTGTATGGGCTCCTTGAAGAGGACCAGAAAGGGGAGACCTCTGGCTAGGCCCACACCAGG
GCCTGGCTGGGAGTGGCATGTATGACGTGACTGGCCTGCTGGATGTGGAGGGGGCGCGTGCAGGCAGCAG
GATAGAGTGGGACAGTTCCTGAGACCAGCCAACCTGGGGGCTTTAGGGACCTGCTGTTTCCTAGCGCAGC
CATGTGATTACCCTCTGGGTCTCAGTGTCCTCATCTGTAAAATGGAGACACCACCACCCTTGCCATGGAG
GTTAAGCACTTTAACACAGTGTCTGGCACTTGGGACAAAAACAAACAAACGAAAAACATTTCAAAAGGTA
TTTATTGAGCACCTGCAGGCGTGACCTGACAGCCCAAGGGTGGGTGGGGTGAGGGCTTGAGGACTTGGGC
GGGACACAGGCTCCAAACTGGAGCTTGAAATAGTGTCTGATGAATGTTAAATTATCTATCTATCTATTTA
TTTATTTATTTGAGACAGGGAAAGGGTCTCCCTCTGTTGCCAAGGCTGGAGTGCAGTGGCGCAATCTTAA
CTCATTGCAACCTCCACCTTCTGGGTTCAAGCGATTCTCTTTATTCAGCCCCGGGAGTGGCGCGCGCCAC
CACGCCCAGCTAATTTGTGTATTTTCAGCAGAGACGGGGTTTGCCATGCTGGCCAGGCTGGTCTCGAACT
GCTGGATTCAAGTGATCCGCCCATCTCCGTCTCCCAAAGTGCTGGGAATTACAGGCGTGAGCCACCAAAC
CCGGCCTGATTAAAGTTAAATAAATACTAGTTCCCTTCTCGTCCAAAGGAGCAGGGAATGGGAACCGGGA
AGGCACGAAGTCTCTAAAGCATCCAGAAGACCCCTACACCAGGGTCTGGTCCGCTCCTATTCGCCGCAGC
CTTTCTGTTCCGCCTGCAACCCATTTTCCAGACAGTAAAACGGCGGCGCACTTCTTTCTCCGTCAGGCAC
CAGGTCATAAGGAACCCAAGAGTCTGTGCCTCTGAGGCCCAAATTATTTGCTGTTTCCTCAGGGGAGCCG
GCGGCCGCGACTCCCACGCCGCGCCGTTACCGCTCCCTCTCTGCTGACTGCTCCCCCTAGGGGCAGAGAC
GGTCCCGACGCCCGCCATCCCGCCCCGGCCTCACCCCTCCCCGCCAGGCGGAACGACGCGGGGAGGCGGG
CGCTCGGGGCTCGCGCCAGGGGCCCCAGAATCCTTCGGGGAGAGTGGGTGGGAGGAAGCTGTGTGGGCGG
GGAGCCCCCTCTGCCTTAGGGAGCGGCTGGGCACCCATTCGCCCCATTCAGGGGCTGCACTTTATAGACG
TTCCCTAGGCTGTTTCTAGGCTCCCCCAAGTCCCTCCTCCAGCCTCGTCGGGTCCCTCAGACCCCAGCCC
AGGACCTGCGGAGGGCCGCAGCGAGGAGAGGCCAACAGGCCTTTCCCTAGAGTTGAACCTGGGCCGGGTG
TTGCACCTGGAAGAACCCCCGATTTCCTGGGGACCCAGCAGGGCAGGCGGCCTGGCTCCGCGCTCAGGTC
CGGACGCTTGTTTATGAGAAGAATTTCCTCTTTCTTAAAAGGGCAACGATGCGAGTGGGTCCCTCAAGGA
GAGAAGAGATGGGACCGGTCTGGTGCGACCTGGGCAAGCGCTGCAGAGGGTACCTGGGCAAGAGGGCCGC
CCGCCTCCTCTGGGTTTGGCACTGGAGAAGATGGGTCCATGCCAGCTGAAGGAGGAGATGGATGGGGGAC
GTTTAGCGAAGAAAGGCATCTCCCAGATCCTTTAGCCTCCTGGAAGTGCCCCCGTTGTACCCCCTACACA
CCCCTCTTGGCATTGAGTGCCAGTCCTCTGCCAGGCTCTGTGTTACAAGTTGGGGAGGGCGGCAAAGTCC
CGAATTAAAGATGTCAGTTCTCAAGG

Protein Sequence : Show Sequence

>gi|109255241|ref|NP_000569.3| natural resistance-associated macrophage protein 1 [Homo sapiens]
MTGDKGPQRLSGSSYGSISSPTSPTSPGPQQAPPRETYLSEKIPIPDTKPGTFSLRKLWAFTGPGFLMSI
AFLDPGNIESDLQAGAVAGFKLLWVLLWATVLGLLCQRLAARLGVVTGKDLGEVCHLYYPKVPRTVLWLT
IELAIVGSDMQEVIGTAIAFNLLSAGRIPLWGGVLITIVDTFFFLFLDNYGLRKLEAFFGLLITIMALTF
GYEYVVARPEQGALLRGLFLPSCPGCGHPELLQAVGIVGAIIMPHNIYLHSALVKSREIDRARRADIREA
NMYFLIEATIALSVSFIINLFVMAVFGQAFYQKTNQAAFNICANSSLHDYAKIFPMNNATVAVDIYQGGV
ILGCLFGPAALYIWAIGLLAAGQSSTMTGTYAGQFVMEGFLRLRWSRFARVLLTRSCAILPTVLVAVFRD
LRDLSGLNDLLNVLQSLLLPFAVLPILTFTSMPTLMQEFANGLLNKVVTSSIMVLVCAINLYFVVSYLPS
LPHPAYFGLAALLAAAYLGLSTYLVWTCCLAHGATFLAHSSHHHFLYGLLEEDQKGETSG

Molecule Role : Vaximmutor
Molecule Role Annotation : On multivariate analysis, patients with the NRAMP1 (i.e., SLC11A1) D543N G:G genotype and allele 3 (GT)n polymorphism had decreased recurrence time (p=0.014 and p=0.03) after human BCG therapy.
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

37. STAG3

Gene Name : STAG3
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 10734
NCBI Protein GI : 57863310
Genbank Accession : AC005071
Protein Accession : NP_036579
Taxonomy ID : 9606
Chromosome No : 7
Gene Starting Position : 94410282
Gene Ending Position : 94446766
Gene Strand (Orientation) : +
Protein Name : stromal antigen 3
Protein pI : 5.05
Protein Weight : 132581.25
Protein Length : 1225

DNA Sequence : Show Sequence

>gi|157734172:94410282-94446766 Homo sapiens chromosome 7, alternate assembly HuRef, whole genome shotgun sequence
CCGCGCTTTTCTGGAATCTTTCGCCCCCGGAAGGGCAGCGGCGGGCGCCTGTGTGGAAGGTGGGGTGGCC
AGAGACACCCGAGGGACCTGAGGTGGGGACCTTCCCTGAACCCTGCTCGGCCAGAAGTCACTCTTCCAGG
CTCTGACAGTGAGGTTTCTCCTTGACCCAAGAATAGGCCCTCTCGCGACCCTCCCGGCCCTGACACTAGG
AGCCTTCCCGGGCCGTGCCCCCAATGGCCTCCGCCACAGAGCTGGAGAATCCTCTCCCCAGGCCTCCATA
GACTGAGAGTCCATTCTCTTAAGCGCTTGGAAGGCTCTTTATCCTGGAGCCGCTTACCCCAGGGGCAGTC
GTAGATATTTTTACCTTCTGGAAAAATAGGAAACGCGCACCTGCCTTTCCTCCTTTTGCGTTTCCAGCCC
TATTCTCATGGCGCCTCCACCCCCAAATCCTTAAGAGCCTCCTGTTCCATTCCAGGTGGAATCAGGTGCT
AGTCCTCTGGAAACGAAAGCCTGGGGTCTTCTTGTATTTTTTTTCTTATTTCTTTCTTTATTTTTTTTAT
TTCATTTTAAAAAACCTGTAAGTTCCCTTGCAGAGGGCGGTTTATACTTCCGAAGCCTCCAGAGAGGGGT
GCGTCTGAAGCTGCCCTGCTGTCTGCCTTGCTTGGCTTTTTTTTTTTTTTCCATTTAATTTTTTTTTTTT
AGAGACAGGGCGTTGCTATGTGGCCCAGGCTGATCTTGAATTCCTGGACCCAAGCGATGCTCCCGCTTCG
CCCTCCCAAAGTACTGGGATTATAGGCGGGAGCACTGTGCACCACGCCTCGGCTTTTGGAGAGCAATTCC
AAACCCACTGCCCAGTTTCTGGTGTCTGTAATCCCTTGGTGCTTTAGGTCCACTCTTTGCCTTATTTTAT
TTATTTGTCATTACTTTTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTTCCCAGGCTGGAGTGCAGTGG
TGCAGTCATAGCTCGCTGCAGCCTCGACCTCCTGGGCTCAGGCGATCGTCCCGCTTCATTCTGCCGAGTA
GCTGGGACTACAGGTGCACCACCACACCTGGCTAGTTTTTTTGGTTTTGGTTTGGTTTTTTCGTAGAGAC
AGGATCTTGCTATATTGCCCAGACTGATTTTGAACTCGTAGGGTTAGGCAGTCCTCCTGCTTCAGCTGCT
CAAAGTGCTGGGATTCCAGGTTTGAGCCACCCTGGGCACCTTGCCTTTTTTTTTTTTTTTTTAATTATGA
GTTAAATTCCCTTGCAGCAGGCAGTTTACACTTCTGAAGCCTCTAGAGAGGGCTGTGTCTGAAGCTGCCC
TGGTGTGTACCTTGCTTGGGTTTTGTTTTTTTTTTTTTGGGTGTGTCTCATGTTGCCAAGGCTGGTCTCA
AACTGCTGGGCTCAAGCAGTCCTCCTGTCTCAGCCTCCTGAGTAGCTGGGACCACAGGTGTGCGCAACAA
CACCTAGCTAGTTTTTGCTTATTTTTTGTAGAGATGGGGTCTTGCTATGTTGCCCAGATTGGTCTTGAAC
TACTGGGCTCAAGCAGTCCTCCCGCCTCAGCCGCCTAAAGTTCTGGGATTACAAGTATGATCTACCACAC
CCAGTGTGCAATTTTTTTTTTTAAAGAAAATACCTATGAGTTAAATTTCCTTGCAGTAGGCAGTTTGCAC
TTCCACAGCCTCTAGAGAGAGATGTGTCTGAAGCTACCCTGGTGTGTGACTTCCTCAGCTTTTGGAGAGC
AACTCCAAACCTGCTGGCCAGTTTCTGGGGTGTTGTTGTGCTTAATCCCTTGATGCCTTAGGTCAGCTCT
TTGTCTTATTTATTTATTTATTTTGAGATGGAGTTTCACTCTTGTTGCCCAGGCTGGAGTGCAGTGGCAC
AATCTCAGCTCACCACAACCTCCGCCTTCTAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCAAGTAGCT
GGAATTACAGGCATATGCCACCACACCCGGATAATTTTGTATTTTTAGTAGAAATGGGGTTTCTCCATGT
TGGTCAGGCTGGTCTCGAACCCCTGACCTCAGGTGATCTGCCTGCCTCGACCTCCCAAAGTGCTGGGATT
ACAGGCATGAGTCACTGTGCCCAGCCATTTGTCTTATTTATCATTACTATTTTTTTGAGGGGGGACAGGG
TCTTCTTCTGTCACCCAAGCTGGATGGAGTACAGTGGTGCGATCGTAGCTCACTGCAGCCTTGAACTCCT
GGACTCAAGCAATCCTCCCACCTCAGCCTCCTGAATAGCTGAATACAGGCATGCTCTACCACACCTGACT
AATGATTATTATTATTTTTTTTTTAGAAATGGGGTCTTGCTGTATTGCCCAGGCTGGTCTTGAACTCCTG
GGCTCAAGCCATCCTCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAGGCATGAGCCACGGTGCCCAGC
CTTTGTCTTAGTTTTTGGTCCCCCTCACCTGACCCCACAGTTCTAGAACCATTCCCTTGGAAAGAAAGGG
ACTGGTCAGGGCTGGGGATGGAGAAGTGCTGTGGTAGGAGCCCTTTCTTCTCTTTCTTCCCCAGCTGGAT
CGCCATACCTACCCTGTGGTCCTCATCTTCCTGGCCTCATAGCTCCTCCTCTCCAAGCATGTCTTCCCCG
TTGCAAAGAGCTGTGGGAGATACCAAGAGGGCCTTGTCTGCATCTTCTAGTTCCTCTGCCAGTCTACCCT
TTGATGACAGGGACTCAAACCATACCTCAGAGGGGTAAGTAGATGTTGCATTGTGTGGCCACTTCCTGTG
TCCCTGGCAGCCTTCCCCCTCGTTTTCCCACATTGATGATAATATGCGTGGGACTGTGTGAAAACACCAG
AAGTGCTATACTCGTTCAGCACAGTGACACCCTCCCTCCAGCTCCAGGTGTTTTTTTTGGCGGTCCTAGA
TGTTGTAGACATCAAAATAGTAGTACATCCTGTTTTTTTTTTTTTTTTTGAGACGGAGTCTCGCCCTGTC
GCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTTACTGCAACCTCCGCCTCCTGGGTTCAAGCACATCC
TGTTTTTAATCCATCATCCATCTTGTCTAAACCTCTCTTAAACACATTTCTATGTTCAGCATGTACCAAC
CCTGGTGGGGGCTGAGAGCTGGCAGATGGGGGAGTAAAGAGTTCTTTATTTGTTGCCTGGTAAATTGTAT
ATACTTTTTGGCTAGTGTCCCCTATTTCTTCTACTTGGGGATTTTGTAAATAGGTCTGTGTATCCTTATT
TATTACCATATATGAGTTTATTCTGTGTAGGTTATAATAATATATCTTGGTTTTTTACCTTTCCAGAAGG
AAAAAAGCGAATTTCATACAGATCTGCTTTATCCCCTGGCTGTTTTAATTGGCTTTCTCTGAATCATTTC
TGTTTCTGTCAGTGCTGCTGTGGGGGACCAGATTATATCAAAGTTATAAACTTGAATTGATAAGACATGT
GGTTTATGTTTCAAGGGGGTGAAATTTAACTCTAGATACATATTACTCTCCCATTTATGGATGAATGATA
GATGACTTCCTGAGCCATGATTATCTTTCTCTACAAAGAGCAGTGATCTCGCTACCTGCTAAGTGAGTAT
CATTTGATTCTCTTCTTCGGAGAGAATACTAGGAAGAGTTGTCCTTCTCCTGCCTCATTTCCTCATTCTT
CGTCTCCATTGCTTTCACGTGAAAGTTTTCCTTTAAGGCTTTGTCCCGGCCGGGTGTGGTGGCTCATGCC
TATAATCCCAACACTTTGGGAGGCCGAGGCGGGTGGATCACCTGAAGTCAGGAGTTTGAGACCACCTTGG
CCAACACGGTGAAACACCTGTAATCCCAGCTACTCGGGAGGCTGAGGTAGGAGAATCGCTTGAACCTGGG
AGGCGGAGGTTGCAGTGAGCTGAGATCATGCGATTGCACTCCAGCCTGGGTGACAAGGGTGAAACTCCGT
CTCAAAAAAAAAAAAAAAAAAGACTTTGTCCCAACTTCCTACTAACAATAAGCTCATTTTTTCTTAAAAT
CTGGATATGTTTTTCTCATGGAGATGGGGTGCGGTGAGAGACAGAGAAACCATAATTAATTAGCCTTTTA
TATGGAGGGAATAGGGTGGTTATATTTAAAGAGAACCATACTTTCTCACAGGAATGGCGACTCTTTGTTA
GCTGATGAAGACACTGACTTTGAAGACAGCTTGAATCGCAATGTGAAGAAGAGAGCAGCAAAACGACCAC
CGAAAACAACACCGGTGAGTCAGCCAGTTTTCTTTTGTTTTTGAATCTTGTGGGGGAAGCAGCCCAGCTA
CTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGGGGCAGAGCTTGCAGTGAGCCGAGATTGCGCC
CCTGCACTCTAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAGAAAAGAAAACCTAAAA
GAGGTTCAGGTGATACGGTTCATAAAAGGACAAGATTTACAAAGTATTAAGGGATGGAAATAGGAAGAAG
AGAAATTTTGAAGAGAAAAGAAGAGAAAAGGACGTTAGGATGTAGAGATACAGGAAGGGGACTCAAGGAC
TTTTTTTTTAAGGGAAAAAAATAAACTTCATAACAATATAAAAATCAATTATTTCAGGACTGAGTCTAAC
TCAGACTCTGCTGTGGAATTGGCAGCTTAAGCCTACTTGTGTGATAATGATGAAACCAAGCGTTAATGTC
ACTGTTACCTTTTTTGTTTTTTTTTCATATTTCTGATCTTTTTATACATATTAGGTGGCAAAACATCCAA
AGAAAGGGTCCCGAGTGGTACATCGTCATAGCCGGAAACAGTCAGAGCCACCAGCCAATGATCTTTTCAA
TGCTGTGAAAGCCGCCAAAAGTGACATGCAGGTAAAGCAGTGTCTCACCTCTGTTGATACCATCTCACTT
TTTGTAAGGTGGTAAGGACAAGTGTCTACAAACTTGATTTGACGTGAACATTTTAGTGAGTGTAACATGT
CAAGAAAGATAAGGTTATGGGGTGAATCCTTGGAGTGATAGAAGGGTAACCTTGAATGAGAGAGCATGTT
CTTTTTTTTTTTTTAGACAGAGTCTCTTTCTGTCGCCCAGGCTGGAGTGCAGTGGCACAGTCTTGGCTCA
CTGCAGCCTCTGCCTCCTGGGTTCAAGCGATTCTTGTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGC
GTGTGCCACCACGCCCAGCTACTTTTTGTATTTTTAGTAGAGATGGGGTTTCGCCATGTTGGCCAGGCTG
GTCTCCAACTTCTGACCTCAGGTGATCCGCCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAG
TCACTGCACCTGGCTTTGAGAGAGCATGTTCTTTTCTTAAAGGTATCACAGGAGTGATTATGCCTTTATT
GATTGATGGAGCAGATGAGGAGAGGCATTGCATGACACAGGAAGGAAGCTTGGATGTGGGTGGCACCTTG
TTCACCCATGTTATTTTTTAGCTCCATCTTGACAATTATACTTAATTATTTTTTCCTTGTTTCTTTTTTT
ATAATTTTATTGAAGTTCTAATACCCGAAAGTGTACAAAGCTTATATGTAGTTTAAAAAATAAAAATGAA
ATGATTACTCAGATCTCTAGCCCCAGCTCAAGAAATGAAACATTAATAGTGCTGTAGAGATCCTTTGTGT
ACCTTCTTTCTACTCCATTTCACAGTCTCCTTATTACCCACAGGTAAACACTAGAGTGAATTTTGTGTTA
ATCTTTCTCATCTCTCATTATAGTGTTACCACTATGAATATGTTCTTTAATTATGTAAATGAATCCATTA
TTCAGGCTTCTATGATTTAATTATTTGAGTTAACATTGTTTGAGATTTACCCACATTAATGTGTAATTTT
ATAGTTTCTGGGAAAATATTTAAAATTTTCAAACATATAGAATTTTGCTAGTTATATTTTCGTTATTTGA
TTTCTAACTTCATTGTGTTGTAGTCAGGGAATGTGGTGTTTGAAATTTATGTAGGGGCCGGGCACAGTGG
CTCACACCTGTGATCCCAGCACTTGGGGAGGCTGAGGCGGGCAGATCACTCGAGGCCAGGAGTTTGAGAC
CAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGCATGTGGTGGCGTTT
GTCTGTAATCCCAGCCACTTTGGAGGCTGAGGCATGAGAATTACTTGAACTCGGGAGGCGGAGGTTGCAG
TGAGCTGAGATTGTGCCACGGCACTCCAGCCTGGATGACAGAGTGAGACTCTGTCTCAAAAAAAAAAAAA
GAAAGAAATTTATGGAGACTTAGCCTTATGGCCTAGTATTGAGTTGGTACATTCGTTTTATCTTTTCTGT
TTCTTTTTCCACCTTTGCTTGCCTTCCTTTGGATTTCTTATCTTCTGCAAGGACCTGAGCACATTTTAAT
TCCAAATGTCTTTTCCACCTTATATGCAGTTGTACAGCGTGTTAGGTTTTTTTTTTTTTTTTTTTTTTTT
TTGAGACGGAGTCTTGCTCTGTCGCCAGGCTGGAGTGCAGTGACGTGATCTTGGCTCACTGCAACCTCCA
CCTCCCGGGTTCACATGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGATGCATGCCACCAC
GCCCAACTAATTTTTGTATTTTTAGCAGAGATGGGGTTTCACCGTGTTGGCCGGGATGGTCTCGATCTTT
TGACCTCGTGATCCGCCGGCCTCGGCCTTCCAAAGTGCTGGGATTACAGGTGTGAGCCACCATGCCTGGC
CTTTTATTTCTTTTTTTTAGCCCATTCTCCCCCTTTTATTTTTAAAATTTTATTAAACATAGTCATTTTA
TAATCTATGATAAATTATTATCTAAAGTCTTTGTGAGTATAATTCTGTCAGTGCCTTGTTTCTTTATATG
TTTTACTAGGTTTTATTGTGAGCCACTCATTTTCCCTGGAGTTCTTATGTGTTGGAATTCTTTGGGGCCT
AGGCCAAAGCTGGGTTTTTAAGAAAGGAACCTGCTTTTGCTTTGACAGTTGTCTGGGGACACTCCAGAAT
CACTTTTTTTTTTTTAAGACAGGGTCTCATTCTTTCTAGGCTGGAGTGCAGTGGTGCTATCACGGCTCCC
TGCTGGTTTGACCTCGCTCAGTGGCGAGGTGGTGATCTTCCCACCTCAGCCCCTCTCATAGCTGGGACTA
TAGGCATGTGCCACCATGCCTGACTAATTATTCTGTTTTTGGTAGACCAGCCTGGGCAACATGAGTTTCA
CTTTACTTGGGTTACTGCTCCCAGCCTGAATCGCTTTTAATTATCTGAATGATTATTCTGGGCTGGGTGT
GGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTAAGGTGGATCTCACTTGAGGTCAGGAGTTCGA
GATCAGCCTGGCCAATATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCATTAGCCAGGCGT
GGTGGCGCACACCTGTAATCCCAGCTACTTGTGAGGCTGAAGCAGGAGAATTGCTTGAACGTGGGAGGTG
GAGGTTGTAGTGAGCCGAGATCACACCATTGCACTCCAGCTTGGGCTACAGAGTGAGACTCCATCTCAAA
AAAAAAAAAAAAGGAAAAGAAAAAAAAAGATTATTCTGATTATTCTGATCATGTATGTGGTGTGAATCTT
GATTGCAAATCCAGATCTGAGGGCTCACTATGGTTATGAATTCTCAAGAAAGAATTATTTTTTTTCCCTC
CACTGTACCCTCAGGTCATAACAAGCTAGTTTTCTTGCTGTCTCTATCTGCCCGGTAGGTTTAGTGTTCA
ATCTGACTTCCTTGAGGATCTGACTCTTTGCAGGACCCAGCTTTATTTAGGTGCCTCCTCTTGGACTACT
CGTCTTGGAAAAAGCTTAGGCTCTACCTCCTGTGCTCTGCATTCCATGCAGCCATCTGGATACAAAGTCA
TAAATCCCCAGGGATTAATGAAACCTTTAGGGCAAAAGTCAGTTTGTTTACCTCCCAGGGTTGCTACTTA
CACCTCATTTTTGGCCTATATGAAATTTTACCTTTCATTCTAGTTCACCAGTACATTGTGAAAGATGTTT
TAAATGTTTTACAGAAGTTTAGATGGTTCAACTGGGAAGATCATATAGGATTTCTATTCTGTCATATTGC
CAGAAACAGAAGTCATCTACATTTTTTTTCCCTAGTCTTTGGTAGATGAGTGGCTGGATAGCTACAAGCA
AGACCAGGATGCAGGATTTCTGGAGCTTGTTAACTTTTTCATCCAATCTTGCGGATGTAAAGGTGAGGAA
ACTGCTCCCCCTTTCTAATTCCCAGCCTTTTGTTCCTATGTTATGAAATTCTTTCTCCATTTAGATAAGT
AGGATTAGACATTTCCTAAATAAAGGAAGATGGTAAAGCATTAGAAAAATTTTACTTATTTAGGCCGGGC
ACTGTGACTCACACCTGTAATCCCAGCACTTTGGGAAGCTGAGGCAGGTGGATCACTTGAGGTCAGGAGT
TTAAAACCAGCTTGGGAAACATGGCGAAACCCCTACTAAAATACAAAAAATTATCTGGGTATGGTGGCTC
ATGCCTGTAGAATTGCTTGAACCCAGGAGGTGGAGGTTATAGTGAGCCAGGATAGCGCCACTGCACTCCA
GCCTGGGCGACAGAGCCAGACTGTCTTCAAAAAAAAAGAGAGAAAAGTTTCACTTCTTTAACTAAATTTT
TTATTGATAAATATGGGAAGTGATCCAGTAGGGAACCATGTTGGTTTAGAGGCAAGACACTTTAGGGAAA
AAGAAGTAATCACGCTTATGTTATGGCACAAATTTGAAGACACCCTTTGGTCTTTCTGGATGCAATAATG
TTACTCTTTGTTTCCCAGCTACTTTGCCCATATTTCTCCTCTGTAGCTGTGGTACCCCCAGAACTCTTAT
TGCCATGGCTTCGTTTATTGGGACAGATGGTCTCAGTCAGGTTGTATTATATAAGAAATCAGCATCTGGC
TTAGTTTTATTGCTGTCTTTGTTTTGTTTTTTTTTGTTTTTTTTTGAGACAGAGTCTCACTCTGTTGCCC
AGGCTATAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTCTACATCCTGGATTTAAGCGATTCTCGTG
CCTCAGCCTCCCAGGTGGCTGGGATTACAGGCGAGTGCCACCATACGTGGCTAATTTTTGTATTTTTAGT
AGAGATGGGGTTTCACCAGGCTGATCTTGAACTCCTGACCTCAGGCGATCCCCCTGCCTCGACCTCCCAA
AGTGTTGGTATTACAGGCATGAGCCATCGCGCCTGGCCTGTTTTTTGGTTTGTTTTTTTTTTGTCGTTGT
TGTTGTTGTTTTTTGGCAGTTTTTCACTGCTTGATTCCTTTTTTTTTTTTTGAAACAAATCCTGCCTCAG
GTTTATTTGTACAAATAGTGCAAGGGGACACCAGTCCTGACCTCATGCTGGCAGAGAGAGATCTCTACTC
TGAAGCCTTTGTACAGGCCTGGGTACTTTTGGGAGCCTGAGCTGGAACTGAAGGTGGAGGTGCAGCCTGG
CCCTTCATTTGATCCTTGGCCTTTGGCTGGCACAGCCTGAGCCCCTTGGCATTGTGGGCACAAGCATGCT
TCTCAAGCTTGGGGTGGGCAGTGTAGGCAAGTCGGCTTAACCTCCTTGGGCTTTATGAGGGACTTGATAG
CCTCAGCATGTGCACTCATGGCCCTATGGCCCTGGCATTGTTGCCCTGCATCATCTTTTTTTTTTTTTTT
TTTGAGACAGTCTCACTTGTTGCCCAGGCTGGAGTGCAGTAGTGCGATCTCAGCTTACTGCAACCTCCGC
CTCCCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACCCGCCACTATG
CCCAGCTCATTTTTTGCATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAATTCC
TGACCTTGTGATTCACCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGACCGACTGCACCCGGC
TGGCCTGCATCTTCTTTAGGCCGTTCTTGTTGTGCTTCTTGGCAAAGCACATATTCCTCAGGAATTTGGG
TCTATCCGCTTAAGAGATTTGTATCTTTGTGATTGGGGTTTCTTGATGCCATTTCTGTGCAGTTTTCAGC
CTGGTTGTGTGAGGTGTGGTTCTTGGATTTGGCCATGTCTACACCATAACCTGTGGCTCCCTTCCTTTGG
GTTTTTAGAGAGATTGCCTTATAGCAGATACATGGTTGGATGTTGGCAGACTGTTGACCAGGGGGTCTTC
TCATTCCCCACCTAAGCTCTTTGCAGAATGTTTCCTTGCTCATCTTCAGGTATATCTGTAAATGATCAAA
GCATCCTGTTATTTTCCTTGTAAGCACCTCATATCCTTCATTCTTTAGGCATTGTGACCCCTGAGATGTT
CAAGAAGATGTCCAACTCAGAGATCATCCAGCACCTAACAGAGCAGTTTAATGAGGTGGAAGAAGATGAC
CAGGATCCTCTTACCCCTCTTATTTCAAAACACAATGCCTATTATCCCCTTCTTTTTGATTCTGTGAAAT
AGGGTAGCTTTTAGAAGGAGGAATTCAGTCTCTTGGGGGTAAAAGAGATCTGAATATGCCTCTCAAATTG
GGGAGAATACCAGGAAATGAGGTATAACAGATATTTTTACTGGACTGTTGAGTTTTGACATCCAAGCCCC
TATGACTTCATGGACCTGGTAATAACTTTCCCATCCTTTTCATACATCCTTTTGTAGGACTCGGGGGACT
ACCCTCTCATAGCTCCAGGTCCATCCTGGAAGAAGTTCCAGGGCAGCTTCTGTGAATTTGTGAGGACATT
GGTCTGTCAGTGCCAGTACAGCCTCCTCTATGATGGCTTCCCTATGGACGACCTCATCTCCCTGCTCACT
GGCCTCTCAGACTCACAAGTCCGCGCCTTCCGTCACACTAGCACCCTGGCTGGTGAGCATTCATTTTTAC
TCTGGACATTCTCCTGGGGATTTATAGGACTTTCCTCTGTTCTCTGATTCAGGATCTTCTTTCCTACCTG
CATCTTGGCTCTTCACTTCAAGGCCATGCCTCTTTTATCCTAAACTTCAATCCAGTTTCTCACTAGTGGA
GTTTTGAGAAGGGGTAGATCACAGCAACAATTTCCAGGTTTAATATTTTTGTCAGAGCTCACCACTAGGG
GTATGCTGATGGTTAAAAGAAGACCACAAGGGTTCGAGGGAAACAGTCCACTCCAGGACAGGCCGTCTTA
GGATTTTAGGGTCATCCTGCTCTGACTTCTCATTTTCATTGCCCTTCTTTCCTTCTATTTTTTCCTTCTA
CTCATCCTCTCTCCTCTGACCTCAGTAATGATTTCTTTATCTCTTTTTCCTTTCTCAAAGCTATGAAACT
GATGACCTCCCTGGTAAAAGTTGCCCTCCAACTGAGTGTGCACCAAGATAACAATCAGCGTCAGTATGAG
GCTGAAAGAAACAAGGGGCCAGGGCAGAGGGCACCTGAGCGGCTGGAGAGCCTGTTGGAGAAACGCAAAG
AGGTGAGGAGTGTTCCCTGCTTCTTCCTTCCCTTTTTCCCAACTTGCACCCACTTCTGCCACAGACCCCC
TTATCAGGCCCTGGGCAGTCTTTCAAGATCATGAATAATGGAGTCTGGCAATAGTTTCATAGACCCATCC
TGTAAGGGGTCATTACATGCTCATCTTGTTTCTGGGTTGTGGGCTCAACAAATAATGTGTAATTTAGAGT
TCTCTCTAATTCCACTTTTGCTCAGTTTTTTTTTTTTTGTCAGCACTCATTGTTAGGAGTGTTTAAGTGC
TGTACCTGCCTGTCATGCAGGGAATTAAGTTTTGACCTACTTGTCAAGTGTGCAGTGTGCAAGTGAGTGC
AGGAGTACTAAAAGCCAACCATTGACAAGGGAGGCCAAATTTTCAAGGAATGGAAAGAAAACAGGCCTCC
CAGTGAACTCGCTTTAAATTCTGTTGAATTTAGTCACTAGCTCCATAGCCTTATGTAACCTCTCTTCAGT
TTTCTTTTCTTTAAAAAAAGGAATAATCTCACCTTTTTGTGAAGAATAAATGAGATGAGTAAATTGCCTC
GCTTGGCTCATAGTAGATACTTGTTTTGTTTCCTTTTAAACTTAAAGATGACCACTGGGGGGCATAAGCC
ATAGCAAAATCTTTTACGCTGATGAAATCTTTAATCAGCTGGGTCTGTCATAAGAGTAACACTACTGGTG
TTTTCTCCTCACTCTCTGTTAGTGTCTAAGACCCCTCCTATGAGGTTGGCAGGACTCACCATGTCTGACC
ATATCATTTGGCTGTTTCTCTAGCCCTCACAAAAGATCTCTTCCATTCTAAATCACTAGAGAAGGAAACT
TTCCACTTCCCTTATTCTCTTTTGTTATTTTGCATTGTCAGGTTGATCTTAAAAGCTTGAATATTAATCT
ATCCCGGATTTTAAATTATGTACACTAGTGCGAATCTTTTCTTTTCTTTTTCTTTTTTGGCTTCTCTGGG
CTCTTGCTTAATCAATTATCATTATAATTCCCCCATTTTATCCTAAATTTCTTCCTCCCCACTATTTTCT
TCTCTCCACTTATAAATGTATATAGGCTCTTGAATCTTTATTTCACTCTGCTCCTTTGTGAGTTACTCTT
TTTTCCATCCTTCCTTTTAGGATAGTATTTCCTTACTTCCTTAATGTCCAACTAACTGAATCCTTTTTGC
TACCTAGCTTATTTCCCCATTTTCTTTGAAACTGTTCTTTCTAAACCAATGACTTCCTAATTCCTAAAGC
CAGTAGCCTTTTCTCAGTCTCCCTTTCCCAAATATTTTACATATTTGCTTTACCTATTTTTGCTATTTTA
GTGTAAAATATGGACATATAGGTACTTCAGCCCTAAAGACTTAAGTATGTCCTGTCGAAAATGATATGTT
GTTCTTACATAATTGAAGAGGTGGGGCCTGTTGTCTTAAAGGATGTTCCCTTTCTCCTTATAATTCTGTT
TCCTTGGTGTCTATTGTTCATGTGATCCAGGATTTTTCAATAATGGCACTATGGACGTTGCGGGGCTGTC
CTGCCATGGTAGGATGTTTAGCAGATCTCTGGCCTTTATCTACTAGATAGATACCAGTAGTACCCTGCTC
CCCCAGCAAGTTGTGGCAGCCAAAAAAATCTTTAGACACTGCTAAATCTCCCCTGGGGAACAAAATTAGC
CTCAGTTGAGAACCACTGATCTAATTCCTTTCTTCTCATTTTTGCTAGTTCCACTTCTATTAAACTGCAA
CTTACTAAAAATTTGTTTTCCAAATTGTTAGAAACATATACGGGCATCATACCTCAAAGATATTGCAGAT
TCAATTCCAGACCACCATAATAAAGTGAATATTGAAGTACAGTGAGTCACACAATCAAAAAATCAAAATT
TTTAATTCTCAGTGCATATGAAAGTTGTGTTTACACTGTAGTCTGTTAAGTGTGCAAGACTATTGTCTAA
AAAGCAGTGTATGTACCTTAATTTAGAAATATTTTATTGCTAAAAAATGCTAATGACAATCTGAACCTTT
AGCGAGTTAAATCTTTTTGTTGGTGGAGGGTCTTGCCTCCATGTTGATGGCTGCTGACTGATCAGGGTAG
TGGTTGCTGAAGGTTAGGGTGGCTGTAGCAATTTCTTGAAATAAGACAACAGTGAAGTTTGCTGCATCTA
CTGACTCTTCCTTTTACAAAAAATTTCTCTGTAACATGAAATGCTGTTTGATGACATTTTACCCACAGTA
GAAATTCTTTCAAAACAAGAGTCAATTCTCTCAAGCCCTGGTGCTGCTTTATCAGCTAAATTTAGGTAAT
AGTCTGAATCATTTGTTGTCATTTCAACAGTATTCACAACCTCTTCACCAGGAATAGATTCCATTTCAGG
AAACCACTTTCCTTGCTCATTCATAAGAAGCAATTCCTTATTTGTTCAAGGTCATGAAATTGCAGCAATT
CAGCCACATCTTCAGTCTCTACTTCTAGTTCTCTTGCTATTTCCACTGCACGTGTAGTTTACTTCCTCTA
CTGAAGTCTTAAACCTCTCAAAGTCATCCGTGAGGGTTAGAATCAACTTAACTCTTGTTAATGTTGATAT
TTTGACCTCTTCCCATGAACCACAAATGTTCTTTATGGCATTTAAAATGGTGAATCCCGGCCAGGCACAG
TGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGACTACGTGAGGTGAGGAGTTTGA
AACCAGCCTGGCCAACATGGCAAAAACCCATCTCTACTAAAAATACAAAAATTAGCTAGGTGTGGTGGTG
CTGCTTGTAATCCCAGCTACTTGGGAGGCTGAAGCAGGAGAATTGCTTGAACCCAGGAGGCAGAGGTTGC
AGTGAGCCAAGATTGCGCCACCACACTCTAGCCTGTGCTATGGAGCAAGACTCCATCTCAAAAATAAATA
AAATAAAATAAGATAAAATGGTGAATCCTTCCCGGACGGGGGGTTGAGTTTTTCTGGTTTTGTTTTTTGT
TTTGAAGACAGGGTCTCGCTCTGTTGCCTAGACCGGAATGTACTGGTGCAGTCATGGCTCACTGCAGCCC
CAACCTCCTGTGCTCAAGTGATGTTCCTGCCTTATCCTTCCAAGTATCTGGTACTACAGATTGCATACCA
CATCTGGCTAGTTTTTGTTTTGTTTCTGTAGAGACAGGGTCTCACTGTGTTGCCCAGGCTAGTCTTGAAC
TCCTGGGCTTAATCAGTCCTCCCACCTCAGCCTCCCAAAAAGTTGGGATAGTAGGCATGAGCCACCATGC
CTAACCCCAGGAGGTTTTAAATGGACTTTGCCCAAATCATCAGAGGAATCACTGTGGCAGCTATAGCCTT
ATGAAATGTATTTCTGAAATAATAAGACTTGAAGTTGAAATTACTCCTTGTTTCATGGGCTGCAAAATGG
ATGTTGTGTTAGTGGGCATGAAAAAAGCAGCATGGATCTCCTTGTACATCTTCACCAGAGCTCTTGGGTG
ACCAGGTGCATTGTCATTGAGCGGTAATATTTTGAAAGGAATCTTTTTTTCTGAGCAGTAGGCCTCACCC
ATGTGCTTAAAATATTCAGGAAACCATACTGTGAACAGATGTGATATCAGTTAGGCTTTGTTGTTTCATT
TATAGAGCATAGGAAGAGTAGATTTAGCATAATTCTTAAGGGCCTTAGGATTTTTAAAATGGTAAATGAG
TATTGGCTTCAACTTAAAGTCAGCAACTGCATTAGCCCCTAACGAGGAGTCAGGCTGTCCGTTGAAGCTT
TGAAGCCAGGCATTGACTTCTCCTCAATAGCTGCGAAAATCCTAGATTTTCCAATATAAGGCGTTTTCCA
ATTATAAGGCAGTTTAGTCCACATTGAAAATTCATTGTTTAGTGTAGCCACCTTCATCAGTGATCTTAGC
TAGATTTTCTTGATAACTTGTTGCTTCTCTATCAGTATTTGCTGCTTCACCGTGTACTTTTATGTTATGG
AGAGGGCTTCTTTCCTTAAACCTCACGAACTAACTTCTAGCTTCAAACTTTTCTTCTGTGGCTTCCTTAC
TTTGCTCAGCCTTCATAGAATGGAAAACAGCTAGGACATTGATCTGGATTGGGCTTTGGCCAAAGGGAAT
GTTGTGGCTGGTTTGATCTTCTGTTCGGACTACTAAAACTGTCTCTGTATCAGCAATAAGACTGTTTAAC
TTTCTTACCCTTTGTGTGTTCACTGGAGTAGCATTTTTAATTTCTTTCAAGAATTTTTCTTTTGCATTCA
TAACTTGGCTGTTTGGCACAAGAGGCCTAGCTATCGATTGGCCTGTCTTGGCTTTCAACTAAGCTTAATC
ATTTCTTTTTTTTTTTTTTTTTTTTCTTTTTCAGACGGAGTCTCACTTTGTTGCCCAGGCTAGAGTGCGA
TGGCGTGATCTTGGCTCACCACAACCTCCATCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGA
GTAGGTGGGGTTATAGGTGCCCGCCACCGCACCCAGCTAATTTTCTGTTTTTAGTAGAGACGGGGTTTCG
CCATGTTGGTCAGGCTGGTCTCGAACTGACCTCAAGTGATTTGCCCACCTTGGCCACCCAAAGTGCTGGG
ATTATAGGTGTGAGCCACTGCACCTGGCCTCTAAGCTTAATCATTTCTAGCTTTTGATTTAAAGTGAGAA
ACATGTGACTCTTCCTTTCATTTGGACACTTAAAAGGGTTATTAATTGACCTAATTACAATATTTTTGTG
TCTCAAGGAATGAATAGGGAGGCCTGAGGAGAGAGAGAGAGACAGAGAAACTCTTGTTGGTAGAGTAGTC
AGAACACAGACATTGTTAAGTTTGCCATCTTATATGGGCACGGTTTGTGGTACCCCAAAACAGTTACAGT
AGTAACATTAGGCTGGGTGTGATGGCCCATGCCTGTAATCCCAGCACTTTGGGAGAGTGAGGTGGAAGGA
TCGCTTGAGCCCAGGAGTTCAAGACCAGCCTGGGCAAACATAGGGAGAGCCCGTCTCTACAAAAAAAAAA
ATTTTTTTTAACTAGCTGGGTGTGGCAGCATGCATCTGGACTCCCAGCTAGTTGGGAGGCTGAGGCAGGA
GGATTGCTCGAGTCCAGGAGGTGGAGGCTGCCGTGAGCCATGGTCACACCACTGTACTCCAGCCTGGGTG
CCAGAGTGAGACCCTGTCTCATAAAAAAATATGTAACATTAAAGATTACTGATCACAGATAACCATAACA
GATACCATAATGAAAAGGTTTGAAATATTGTGAGAATTACCAAAATGTGCCACAGGGACACAAAGTGAGG
ATATACTATTAGAAAAATGGCACTGATAGGTATGCTCAATGCAGGGTTGTCACACACCTTTAGTTTGTTT
GAAAAAAATCCACAATGTCTGCAAATCAATAAAGTGCAATAAAGCGAGATACACCTGTATAATGCCAGCT
AGGGCTTCTGAATAGTCCGAATAACCACATGCAGAGGGGTTATTATTTTTACTAAACACAAACCTCTGAG
GCATTAGTTGACTATACAGCTTCTTCCAGAATTAGAAGGTTTAAAAGAGAGCATAGGATTACGGGGGTTC
ACACTATCCTATAATAAACAGGAAGTAGGTGGAAGTTGTAGTTTTTCTGTATCTTCAGGGCCTTATGCTT
GTTAGGGTACAAGAAAGATTAACCCGTTTCTCCCTGTCCTCCAGCTCCAAGAGCATCAAGAGGAGATTGA
GGGGATGATGAATGCCCTCTTCAGGGGTGTCTTTGTTCATCGGTACAGGTGAGCTAATGGGCCTCTCTCA
TTGAAGCAGCTGGCCTTTGGTTATGGAATCTGAGGAAGTGAATAAGGTTTTCCCCCCTCCAACCCTTAGT
TATTTGGTTAGTCCCAGAATTAATGCAGCTGGAGCAAAAATTCTTGACAACAAATGTTTCAAATTGGTGA
GGTTTAGTTTATCACCACCTACAAAGGATCTTGTAAGTGCTTCTGGGAGAAAATAAGAGAATAAAGCATT
GCTGAGTATTTTCCTGCTCTGGCTTTGCCCCAGATCTGCCCCAACTCCTCATCTTTCTGGGGTTGGAGAC
TTGAGGATAAGAGCAGAGAATAGAAAGGCGGGGCTTTTCTGAGGGATGCCTGCTGCCTGGTGAGCCTTCT
CCTGGCCCAGCCTGCTGTCTCACTGTGCGAAGGGCAGGGGAGTTTTATACTGTTTAATGACCTGATCAAA
TTAAGTTAAAAAGAATATATGTAGGCCGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC
CAAGGCAGGCAGATCACCTGAGGTCAGGAGTTCACAACCAGCCTGACCAACATGGAGAAACTCCATCTCT
ACTGAAAATACAAAATTAGCCGGGTGTGGTGGCGCATCCCTGTAATCCCAGCTGCTCAGGAGGCTGAGGC
AGGAGAATCGCTTGAACCCAGGAGGTGGAGGTTGCGGTGAGCTGAGATGACGCCATTGCACTCCAGCCTA
GGCAACAAGAGTGAAACTCCATCTCAAAAAAAGAAAAAAAAGAAAAAAAAAATTGTAGATGTTTCTCACT
CTTACCTAGGTTTCACTTCTGCCTCTCCAGAGCCTTTCAGCTGATGTAACCTTTTCTTAATGTGGAATTA
AAACACACTCACACACTTTGGCCAACAGTTGAATTAACTTTTTTTTTTTTGGCAGGGACGGAGTCTCACT
GTGTTGCCCAGGCTGGAGTGCAGTGGTGCGATCTCGGCTCACGGCAAGCCCCACCCCCCGGGTTTAGGCG
ATTCTTCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCGCCCACCACCATGCTTGGCTAATTTTTGG
CCACCATGCCCAGCCTGAATGAACTTTCAAAATTGGTTTTTAAAAGAGGTATGGAGACCGGGTGCAGTGG
CTCACACCTGTAACCCCAGCACTTTGGGAGGCCAAGACAGGCAGATCACTTGAGGTCAGGAGTTCGAGAC
CAGCCTGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAAGGAAGCTGGGCGTGGTGGTGCA
CCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCACTTGAACCCGAGAGGTAGAGGTTGCAGT
GAGCTGAGATTGTGCCGCTGCACTCCAGCCTGGGCAACAGGTGGAGTGGAGAATTAGGCTGTACAAGGCT
GGTGTTTAACTATTTTATGGTTTGGTAACAGAAAGAAAATATTTACTAACTGGGTATGGCACATTTTTGT
AGTCCTAGCTACTTGAGAGGCCAAAGTGGGAAGATGACTTGAACCCAGGAGTTCAAGGCCAGCTTGGGCA
ACATAGCAAGAGATCCTATCCCTTAAAAAAAATATTTACATGTATTTTACTATATATATAGTATTTACAT
CGGGAGGAAGAAATGAAAGGCATGGGGTACATGGCATTTCAAGAAAATAAATTCTTCCATGAGAGGGAGT
TATCTGGTAGGATGGAAAGTGAGTTTGGAGAGAGGGTGGCTCTAAGAGTCAACTTATCTGTCTGTGTCTA
GGGATGTCCTTCCTGAGATCCGTGCTATCTGCATTGAGGAAATTGGGTGTTGGATGCAAAGCTACAGCAC
GTCTTTCCTCACCGACAGCTATTTAAAATATATTGGTTGGACTCTGCATGATAAGGTGGGATTCGAGTCA
GTTTCCCTTTCCGTTTCCTTCATCTTTTTCCTGTCCTAGGACATTTCACCTTTTCCTGGGCCTGCCACTG
AGATATTTTACCTAGTGACCAATGATGAACCCATCAAGGCGCTGAACCTTGAAATTCCCCTAGCACTGGG
GAGAGTAGGATAAGGTAGAGAGAGACATACCCAGGAGATGAAATGACTCAAAACCTGAGGTACGGGGAGA
GAGACTTTCCCATGGAAGAGAGTTATTGATGGATGGTAATGCAGGTGCTAAGGGGCAGCCAAAGGATCCT
TCTCATCATGAGTACTGCTGTGATCCTTTTGTTTTTTAAGTAAGAAGACAGCTGTTCATTGTTTCTGACA
TCTTAAGAGTCCTCAGTAGAGGGGACACCCAAGCTAGAATGAGGGATCGGAGAGGGGAGTCTGGAGGCTT
AGTAGGGGCGAGTAGAGTGTGGTTAGTCTTATTTCCATTCTCCTGGTTTTCCCTCCTCACCAGCACCGAG
AAGTCCGCCTGAAGTGTGTGAAGGCCCTGAAAGGGCTGTACGGTAACCGGGACCTGACCACACGCCTGGA
GCTCTTCACCAGCCGCTTCAAGGTAAAATGGGTGGTGCTCCATGGCTTGGCTCTTTGTCGTGGTTCCTAT
TTCCCCACCTTGTATCTTTCTACCCCTCATGCTCTAAGATGTCTTGGCTATCCCAGCATCTGCCTCTACG
TAGGCACTCTCTTTAGGAGTCTCTGATTCTAAGAGAACAGAATGTTTGGAATGGGTGTAATGAGATATTG
AGTGACTTTCTCCTTTCTGCAGGACCGGATGGTTTCCATGGTCATGGACAGAGAGTATGATGTGGCAGTG
GAGGCTGTCAGATTACTGATACTTATCCTTAAGTGAGTCCTGGGAAGAGGGGAGGCCAGTGTCTCAGACC
TTTGCCCTTCCAGGGTCATCTCCCTCCACCTGTCATAGCTGACTCTTCCATCTGTGCAGGTTGACTGAGG
TCATTCCTGAGTTGCAGTATGTTGAGGGGGTAATATTTCTGTCTTCTCTAACTCCCCATACTCCTTTGTC
TTCCGCTCTTCATTTAGAAGTTTTTTGTGAGTTATGTCCTTGTTGCTTTTGCCTCTTTTTGTTTCTAGCC
TTGGTTGTGCCAGAAGACAATGTCCCTATTCACACACTCTTTCTGCTTTTCTGTGGGCAGGAACATGGAA
GGGGTGCTGACGGACGCGGATTGTGAGAGCGTCTACCCCGTTGTGTATGCCTCTCATCGAGGCCTGGCCT
CTGCCGCAGGCGAATTTCTGTACTGGAAGTGAGTGGGGCTCCTTTTATGTTTCTTTAACACCACGCTCTC
GGTTTCTCTCCTCCATCTGCTGCTGCCCTACCTAAGGCTTCCCCATCTCTGTGAAAGTCTCCCTGGTGTC
TCCTTTCTTCTCGCAGCTCCTTGGGGCTTTCCTTAGCTCTCACCTCCTTTTCCTTTATCATCTCCTTGGG
CCATCTCCTCCCTCTGTGGTCGTTACACCTCCTTGTTTCCTGTTGTGCTGAGCCCTTTCCTCGTGTCCAT
TCCACCAGACTCTTCTACCCTGAGTGCGAGATAAGAATGATGGGTGGAAGAGAGCAACGCCAGAGCCCAG
GCGCCCAGAGGACTTTCTTCCAGCTTCTGCTGTCCTTCTTTGTGGAGAGCGAGGTGACATACACAGAGAG
AAGTCTGGCTGTTGTGCATAGGACCTACAAGTGGGCTGGGGTTGGTGGCTCACGCCTGTAAGCCCAGCAC
TTTGGGAGGCTGAGGTGGGAGGATCCTTTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACACAGTGAGA
CCCTGTCTCTATCGAAAAAAAAAAAAAGACAAATGGAGAAGGATGGGAGTGGACACTGTAGGAAGGAGAG
CGATATTTAATCAGTAACCACTTCGTAGGGTATTTTTAACATGCTATCATTAACTCCCCATGCACGTTCT
CCCCTAGCTCCATGACCACGCTGCTTACTTAGTAGACAGTCTGTGGGACTGTGCAGGGGCTCGGCTGAAG
GACTGGGAGGGTCTGACAAGCCTGCTGCTGGAGAAGGACCAGAGTACGTGTCACACGGAGCCAGGGACAG
GGACCTTCCACCCGCTAGGGTGAAACTGGGAGGGATTGCTTGCTTCACTTGTACAAGGCAGCAACGGTGG
CATCGGGTGGGGGGAGCTTGGAGTTGGAAGGTGGCTAATCTTTGATTCTATGTTTTTGATCCTCCTGGCA
CTCCAGACCTGGGTGATGTGCAGGAGAGCACACTGATAGAAATCCTTGTGTCCAGTGCCCGGCAAGCTTC
AGAGGGGCACCCGCCTGTGGGCCGGGTCACTGGGAGGAAGGTATGGTGTGAGGGTAGAGTGGGTAGGTCA
GCAATACTCAGTCTTGACAGGCAATGTGCATCCTTATCCCCCAGGCTGGGGTTAGGGTGCTCCTTCTACC
CTTTAAAGAATTTCATTTCCAGAAAAAAGGGCCAGGCGCAGTGGCTTACGCCTATAATCCCAGCACTTTG
GGAGGCTGAGGCAGGCAGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTGACACAGTGAAACCCTGT
CTCTACTAAAAATACAAAAAAAAAAAAAAAAAAATTAGCTGGGCGTGGTAGCGGGCACCTGTAGTCCCAG
CTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCTGGGAGGTGGAGCTTGCAGTGAGCCAAGATCAC
GCCACTGCACTCCAGCCTGGGTGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAGGAGTTTC
ATTTCCAGAAAAAAGAAATCTCGTGGGAGCTACTGAGTTCTCTTTTCCTTCTAGGCAGATAAGGTCATGG
GGAGGAGGGCTGCACACACCCCCTGCACCAGTGTTTCTTTACACCTCCCCCACCCCCAAGTGACTCTCAT
TCCAGGGCTTAACCTCTAAGGAGCGCAAGACCCAAGCCGATGACAGGGTGAAGTTGACTGAGCACCTCAT
CCCCCTGCTGCCCCAGCTCCTGGCCAAGGTACCGCTGCCCCTCCACTCTGCATCACACCAAGAGAAGTGA
AAGGAAATGGCCTCTGTGGGGGTGGGAGTAGGAATTAGCAATGTGTCAGCTTGTAAGGAGGCCTCCCTGT
CAATCATCACAGTTCTCAGCTGATGCAGAGAAGGTCACTCCCCTGCTCCAGCTTCTCAGCTGCTTTGACC
TCCACATCTACTGCACTGGGCGCTTGGAGAAGGTAGGGAGATAGATTTCCTATACCTTGCTGCTTGCCTG
CCAGGGCCAAAGGGTTCTATTGCCAGTATCTTTTTTTCCTAAGAACTTGGGTTCCAGAAAAATCAGCAAG
CCTTTTCTTAGGCATCTTATCCTTGAAGTTTAATGCTTTTAACCCCGTTCCACTTCCCGCCAGAAGAGTG
TCCTCCTCCCATCCCCACAGCACACCATCTTCTGCCTTTTCTTCTCAGCACCTGGAGCTGTTCCTGCAGC
AACTCCAGGAGGTGGTGGTGAAGCATGCAGAGCCAGCGGTGCTTGAGGCTGGGGCGCATGCCCTCTACCT
GCTCTGTAATCCCGAATTCACTTTCTTCAGCCGGGCGGACTTTGCCCGCAGCCAGCTAGTAGATTTGCTG
ACTGACCGCTTCCAGCAGGAGCTTGAAGAGCTGTTACAGGTAGGAGCTGGGGCTGGACAATGGGACACCC
CAAACAAGGGTGGGAGGGGCTGCAAGACGGGGGATGTGGACTGATGAGTTCTGGGGGAAATGCTATTGTG
GATCTTCTTTCCTTCTCAGTCGTCCTTCCTAGATGAGGATGAGGTATATAATCTGGCAGCCACTCTGAAA
CGCCTCTCTGCCTTCTACAAGTGAGTGGCTTTCCTCCTCTTCCCCATCCCGTTTTTACTGGTGTCTGTGG
AGTTGGTTCCCTCTGTTCTTTTCCAGTATAACATTCCCCTTTCTCCCCCAAAGCACTCATGACCTGACTC
GCTGGGAGCTCTATGAGCCATGTTGCCAACTCCTGCAGAAGGCTGTGGACACAGGAGAGGTTCCTCACCA
GGTGAGTGGACAGGCTAGAGATGGGTTGGGGGCTGGGGGGCTAGATTTTAAGGACCTACGTTCTAGGTGG
CCACTAGGTGTGTCAAGTCTCAGTGCGTGGAGTGGACAAAGCGAGGAAGATCAGGTGGGTGGGGGTCACC
TCTTACATTCCTCTCTTAGAAGGTGGACTCTACTTCCAGGGTCTTCTTGGATTTCCTGGAAGCCAAGGAT
AATATTGAGGGATTCTAGAAGACGTAGAGTAAATTAGGGAGCGGTGACATTTCTACTTCGTGTAGATGCC
TCTGAAGAATGTCCAGAATATCACTCATTTTCCACTGGAAAAGGAATTAGCTCTCCCTTTACTCATTTTC
TCTCCTCTTCACTGGTGTGTTTCTGGCTATCTGTCTCTCCCTCTCCTAACCCAAACCTCATTTTTCAAAC
CCTTTGTTGTTACAGGTTATCCTGCCAGCCTTGACTCTTGTCTATTTTTCCATTCTCTGGACACTAACCC
ACATTTCTAAATCAGATGCTTCCCAGGTGAGTGTGGGTCTTAGATGGGATAATGGGAACAGAGGAGTCTG
TGTCTTCATTCTTCCCCTTCAAGCCACTGTGCCCACAGAAGCAGCTGTCGAGTTTGAGGGACAGAATGGT
GGCCTTCTGTGAACTCTGCCAGAGTTGCCTCTCAGATGTGGATACTGAGATCCAGGAGCAGGTGAGTACT
GACTCAAGTGGGAGCAACAAGGCGAGTATCCCTGCCCCCATTCCAAGGAGAAATTGACAGTGTCATTTCT
ACCTTGGGAAACATTCTGTCCTTTTAAACATCCTTTCTTTTCCCCCTACAGGCTTTTGTCTTATTAAGTG
ATCTACTTCTCATCTTTAGCCCTCAGATGATAGTTGGGGGCCGTGATTTCCTTAGGCCACTTGTCTTTTT
TCCTGAAGCTACTCTCCAGTCTGAGCTAGCCAGCTTCCTCATGGACCACGTCTTCATCCAGCCGGGAGAC
CTGGGCAGTGGTGCAGTGACTCTATACCTGGGGCTCAGTAAGGGCTGGGGCTTGGGTGTAGCTCAGAAAT
ATAGGGCAGGAAGCTTAAGTCTGTGATTCCCTTTTGCCTTCCATGTGAGCCAGGTGATTCCCAGGAGGAT
CATTTACAGATAGAGCGGCTACACCAGCGGCGCCGCCTCCTAGCCGGGTTCTGCAAGCTGTTGCTTTATG
GGGTGCTGGAGATGGATGCAGCCTCAGATGTTTTCAAACACTACAACAAGGTACACCAAGGCCCTACAGA
AATAAAAGAGAAGGGAGCAAGTTGAGCACAGCCATGACTTGGAAACATAATAGCACTACAGGTGGGGGAT
ATCCAATGGTTTCAAGCTTAAAGGAGAAGTAGGAGGGAAAGGCACTGGGAGTTCAGAATAAGGGGACAAA
GAAAAGAGACTTAATAGTAAATAATTATGGAGAAAAAGCTGTGAAGAAATTCACTGGAGGGGAAATAGCT
TATTCTTCCTGTCCAGAAATATTTATTTTTCTCTGACCCCAGTCTATGGAATGGGGTAACATAAGAAGGA
AAAAAGAATAAAAGAGGGATCAGTCCTCACAGGATACCTAGATAGGTGCCCCAGTTTCCTCAACCCACCA
GTATTGATTTATGCTTATTGAGCACCTGCTGTGTGCCAAGCACCATTCCAGGAGCTAAGGCCACAGCAGT
GAACAAAACACACAAGTTCCTATTTGGTGGAGCTTCCATTGTAGTTGGGGAGAGACAAATAGTGAAGGTG
TCAGTGCTGTGTGAGAGGGCACTGTCAGGCTTACTTCTCTTCTCCAAGAATTGGGCTTGGGGCAGTGTTT
ATTTCTTTTCTTTTCTTTTTTTTTTTTTTCCTGAGACAGAGTCTAGCTCTGTCGCCAGGCTAGAATGCAA
TGGCACTATCTCGGCTCACTGCAACCTCTGCCTCCCGGGGTTCAAGCAGTTCTCCTGCCTCAGCCTCCCA
AGTAGCTGGGATTACAGGCATGCACTGCCACGCCCAGCTAATTTTTATAGTTTTAGTAAAGACAGGGTTT
CACCATCGCCAGGATGGTCTTGATCTCCTGACCTTGTGATCCGCCCAACTTGGCCTCCCAAACTGCTGGG
ATTATAGGCGTGAGCCACCATGCCTGGCCTGTTTCATTATTTGTTTTTTATTTTATTTATTTATTTATTT
ATTTATTTTTTGAGACGGAATCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACT
ACAAGGTCCGGCTCCTGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCAC
CCACCACCACGCCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCGTATTAGCCAGGATGG
TCTCGATCTCCTGACCTCGTGATCCACCCGTCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCA
CTGCACCCGGCCTTATTATTTGTTTACCGAAACTCTGATTTATTTTGGGCTTTGCACCTGTTGATTAGAG
ATGAAGTCTAGTCTGAATTATTTTATGCTTCAGTTCATGTTTCCTACCTAAGGGAGTTTGGGAGGGAGAC
ATGATTAGGGATGTGAAGGAAGAAACCAAATGGTCTTTTCCACCAGTCAGACATTACCTTCCCCACTCTT
TCCCCTCAGTTCTACAATGACTATGGTGACATTATCAAGGAAACATTAACTAGAGCAAGGCAGATTGACC
GAAGTCATTGTTCCCGAATCCTGCTGCTGAGCCTCAAGCAGGTGCGCCCTTCTGCCTTGAGGACATGCCA
GCCCTGTTGTCCCCAAGTCTCTGTCTACCCTCAGCCACTCAGAATGTAAGATCCTTCAGAGCAGTAACTT
TTCTTGTTTTGACTCTTAACTTTACGTGATAGGTTCCTTCATTCTTAGTAGACCCTTGGGAAGTATTTTT
TTTTTAACTGACTGGGCAACCCCATCAAAAGTAACTTATCAGGACATTGGAACGCCAACAAGGAAGTGAT
AGGGAGTTCCTAATTATTTTTCAGTCTGTTCTCTCTCAGCCTCCTTCAGTTACTATAGACGTTTTTATTT
AATGTTATGGAGTAGAGGTGATTGGCTTTGTTTGCTGCAGGGGTATCGGGAGTTCCCTAGAAGGAAGGAA
AGAGTAAAAGTAGTAGATGGTGTCCCAAGGCCTTTGGAATTTCTGAGTAGAGAAGAGAATGCTGGACTTC
TCTGTTTCCGCCGTGTTCCTCCCTTTCCCACATGCACCATGTCTCCTGGACACAGCTGTACACAGAACTG
CTGCAGGAGCATGGGCCCCAGGGCCTGAATGAGCTTCCTGCCTTCATCGAGATGAGGGACCTGGCCCGGA
GGTTTGCCTTGAGTTTTGGACCCCAGCAGCTGCAGAACCGTGACCTCGTGGTCATGCTACACAAGTAGGA
AGTATTGGTTGCAGGTTGTGTCCAGGGAGGGCTGGGAACGAGGTCTTGGAGGGAGGTCTCGGAGGGAGGG
TATGTGTGTCAAGGCATAGCAGTCAGGTTAGGGGTGGGTATGCCTTTGGAGACAAGCTGGGGACGGGGGG
AGGGTGCATTTGGACAGGATAGCTCAGGCCTGGGAGGGAAGTGGGAAGAGACTTTCTTCCTAAAATAATT
CTGCCCAACCAAGGGAAGGCATCCAGTTCTCCTTGTCTGAGCTTCCTCCAGCTGGCTCCTCCAATCAGCC
TCCAAATCTGGCATTCCTGGAGCTCCTTTCAGAGTTTTCCCCCCGACTCTTCCATCAGGACAAGCAGCTT
TTGTAAGTTGGTGGGTGGATAGAGATGTGGATTAGGGAAGGGCCTGCTGAGGGCCCACTAGCCCCTTCAG
GCTTTTGGTTCTACCTCTTTCATAGACTGTCCTATCTAGAAAAGTGCCTGCAGCATGTCTCCCAGGCACC
TGGCCATCCCTGGGGCCCAGTCACCACCTACTGCCACTCCCTCAGCCCTGTGGAGAACACAGCAGAGACC
AGCCCTCAGGTCCTCCCCAGCTCCAAGAGGAGGCGCGTTGAAGGTAGGGTGCTGTGTGTGGGTATGGGGG
TGCCCAGCAGGTGGTCGTTGGCTGCCTGCAAACTTATTTTGTCAAGTATTTCATTGAGCATCTACTGTCA
TTCAGGGTATTAATTTCTTTCAAGGTTTATCTAGTCAACTGAAAACCTGTATTAAAAATCAAATAGAGGG
CCAGGTGTGGTGGCTCATGCCTGCAATCCCAGCACTTTGGGAGGCCGAGGCAGGCGGCTCACGAGGTCAG
GTGTTCGAGACCAGCCTGGCCAAGATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCTGGGCA
TGGTGGCGCGCATCTGTAATCCCAACTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAACCCGGGAGGC
GGAGGTTGCCGTGAGCTGAGATTGCGCCACTGCACCCCAGCCTGGGTGACAGAGCGAGACTCTGTCTCAA
AAAAAAAAAAAAGAAACTGATGATAAAACCAAATATTGAACTAAGAAATCTATACATTCAGAGTACCCTC
CTCAACATCTTGTATTCCATTATTTTCGAAACCTTGGACACTTTTACACAACCTTCATTGTTTTTTACCT
TCAGATCACCTGTTGTCTTATAAGTTGCCTAAGGTTTTGTTTTTGTTTTTGTTTATTTTTTGAGATGGAG
TCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCGCCATCTCGGCTCACTGCAAGCTCTGCCTCCCAGGT
TCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCACCCGCCACCACGCCTGGCTAA
TTTTTTTTGAATTTTTGGTAGAGATGGGTTTTCAGCATGTTAGCCAGGATGGTCTTGATCTCCTGACCTC
ATGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGTGCCTGGCTGCCTAA
GGTTTTTTCTTTGCTTTTTAAAAAACAGCTTCATATTAAGCAACATTGTAGCAATCATTATTTCATATGC
AAGTATGTGTTTTCTAGTGTACATTTTTAAATGGGACTTTATGTGACAGCTTTATTGAGATACAACTTAC
ATATCATAAAAATCATTTTGTTTTTTTTGAGACGGAATCTTGCTCTGTCACCCAGGCTGGAGTCCAGTGG
CGTGATCTCGACTCACTGCAACCTCCGCCTCCCACGTTCAAGTGGTTCTCCTCCCTCAGCCTCCTGAGTA
GTTGGGATTACAGGCGTCCACCACCACCCCCAGCCAATTTTTGTATTTCTAGTAGAGACAGGGTTTCACC
ATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTTGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTCCCA
AAGTGCTGGTGAGCCCCCGTGCCCAGCCAAAAATCACCCTTTTAAAGTGTACACTTAGCATATTCACTGA
ATTGTACAACCATCACAACTATCTAATTTTAGAACATTTTCATCTTTCCAAAAGAAACCACATACCCATT
CGCAGTCTCTGCCCATCTTCCCAGCCTTAGGTAGTATCACTAATATAATTCCTATCTCTTGATTTGCCTA
CTCTGGACCTTTCATATAAATGGAATCATATAATATGTGGCCTTTTATGTCCAGCTTCTTTCACTTAGTA
CAGTGTTTTCAACGTTCATCCATACTTTGACATATATCAGTATCTCATACCTTTTTATGGCCGAAACAAT
ATGCCATCGTGTGGATATGCCACATTCTACTTATCCACTTGTCAGTTGATTGACATTTGGGCTCTTTTAT
GCATAATGCTGCTAAACATGCATGTACACATTTTTGGGTGGATGCATCTTTTCAGTTATCTTGAGATATA
CCTGGGAGTGGAATTGCTGGTTCGTATGGCAATTCTATGTTTAAGTTTTTGAGGAACTACCACACTGTTT
TCCAAAGTACCTACACCAGTTTACAATCCTACCAGCAATGTATGGGGACTCTGGTTTCTCCATGTTGTCA
CCGGTACTTATTAATGTCTTTTTTTATTTTATCCATCTCCTAGTAGCTGTGAGGTGTGGTATCTCATTGT
GGTTTAAATTTGCATTTCCCTAATGACAAATGGTATTGAGCATCTTTTCATGTGCTTATTGACAATTTGT
TTATCTTCTTTGGAGAAATGCCTGTTCTGATCCTTTAGCTATTTTAAAATAGGGTTGTCTTTTTATTGTT
GAGTTGTAAGTATTCTTTATATATTCTAGACATGAGATTCCTTATGATATGCAAACATTTTCTCCCATTT
TTTGGGTTGTCTTTTCGTGTTATTGGTGGTGTCCTTTGAAACACGAAAGTTCTTGAATTTGGTGAAATCT
AACTTTTTCCTTTTGTTGCTTGTGCTTTTGGTGCCATATCTTGAGAAATTGTTGGCTAATTCATGAGGTC
ACAAAGATTTACTCCTGTGTTTTCTTCTAAGAGTTATAGTTTTAGCCGGGCATGGTGGCTCACGCCTGTA
ATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACA
CGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGATGGTGGGCGCCTGTAGTCCCA
GCCACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATTG
TTCCACTGCACTCCAACCTGGGCGAAAGAGCGAGATACTGTCTCAGAAAAAAAAAAAAAGTTATAGTTTT
AGCTCTTAATTTAGATTTGTGATCCAGTTTGAGTTAATTTTTGTATATAGTGTGAGATGAGGTGTGATTT
CCTTTTGTTGTCTTCTGTGTACCACCTAAAATTATGTCTTATACCACCAGGAAACTCTGCTTTAATTAAT
TAACTAATACATAGTTGTTAGGGACCTACTATATGAAAGATACTATGTTCAGTGTTTTAGGGGCCATGAA
GAATCCAGCAGCTTATTATCTAATAGGAAAGGGAGAAATTTTAATTATGGAAAACTCGAATTATGAACAA
TTGCCAATAACTGCCTGAGGGCTTGTACAAGTGAATGCTGTAGAATTCAGAGAATGATGAGAGCTTTACC
AAGAAGATGGAATTGCTGGAGCTTAAAAAATAAGTAAGATTTTTATAAGCTGAACAGAGGAAGGAGGAGC
ATCATGGGCTGGCAGAATAGCACAAGCAAAGCATAGAAGGCTGTGTGCTGGGCGAAGCATGGAGGTACAT
AGATCGCTCTGCCTAAAGCGTAGGGTTTGTGAAGGCAAGATAAAACTGAAGAGTTTGTTTTTATTCATTC
AATAGATACTGAGCACCGTGTGCTGGGAATACAGCAGTGAACAAAAAAGCTAAACATCTCTTTCTTTAGA
GAGTGTCAGATAGTAGTAAATACTATGGCAAAAAAGCAGGGAGGATTGTGAGGAATGCTGGCGGGGTGGG
GATTGCAGTCTTACAAAGACTGATCAAGGGAAGACTCAACACAAATGTTGGGCAAAAAGCCTAAAAAATA
AGTTAAAAGATACACTTAGGCACATTACAATTTTAACAGGTTTATTTGAGCATTCAGCAATTTTATCAAT
CAGGCAGTGGCAGACCGCCAGCAGTTCAGGGCGCCACCACCGAGGAAACCAGAGGGGACACTTACAAGGT
GTCTCTGGGAGCAAGACAAAGAAACTATTTGATTGGTTAGAGTGGAAAGTTCATATTTAGAGGTTAACTG
ACCATAAATCTCTTGTTAGAGGTTAGTCGGTAGTTTCTGACTGGTTAAGCTGAAGTTTCCTGCTCCTAGG
TTACACACAACACTTTCACTCTGAGTTGAGTTTCAGTTTGCTGACTTAGGAACCCAAAGTGCAGGAGCCA
TTTCAGCCTATTGGCCTCCCAGCGAATTTTTTATAACAGGTGAGGGAGGGTGCTTAGTGTGTATCAGAAT
AGCATACCAAAGGAAGCAGCCAGTCCTGAGGCCACGAGGGGATGGGGGGAATGTTCCTGGAATGTTCAAG
GAACAGCAGAGAAGACCATTATGGCCAGAATGCAGATTGTAGGACATGAGACCAAAGAGGGTACAGGATC
CCAGAGCATCTAAGGCAGAGACTGGCTTTTACTCTGAGTGCAGTAGGAAAAGCATTGGAGGATTTTGAGG
AACGACAGGATCAACCTCTGTTTTTAAAGGATCCTTCTGGCTGCTGTGTCGAAAACGACAGGAGATGGGG
GAAGGCTAGAAGCTGGGAGACCAGCAGGGAAGTTGTGGCTGTTGTAGGAGTCCAGCCCAAGATGCAAGAT
AAGAGAGCTTCTTAGGCCGGGGGCAATGGTGGAGGTGCCGAGAAACGGCCAGATTCTGGGTGCATTTTCA
AGGTATCTCTAATAGGCTTTTCTGACAAACTGGATGTGGGATGTAAGACAAAAAGAGCAGTTGAGGATGG
CTCCAAGGATTTTGGCCTGACAGAAGGACGGAGTTGACAGTATCTGAGATGGGGAAGGCAGAGAGTGAAG
ATAGCTGTGGGGGAAGGTTGGGGCATTGGTGTTGGCCACAGGATTACAGGTGGAGATGTTGAACAAACCA
TGGGGGCTGTTAAGATGGAGTTCAGGGGAGAGCTGAAGGCTTGAGATCCCTTTGGGATGTGGCAGAGTAT
AGGTGATATTTAAGGACATGAACGTGAGTGGCTGGGGAAGCCAGTGAAGATGGAGAAGAGGAGAGACTCA
AGGGCCCAGCCCTCGGGCATCACACCATTAATAGCTCAGGGAGATCAGGAGGACACATCTGGGGCCCCAA
GGTTTCCTCTCTGGGAATCCAAGCCCAGGGGTATGTCTCTTCCCTGTTTTCTGTAGCCCTCTGCTTAAAC
CCATAGGCTGAGCGCTCACTTTGGGATTATTGTTTTTAGTGGAGAAGGGGGGTAATGGGAGTGTGACTGT
TTACAGTTAACAGATGTTAATGTCAATTTGACTGATGCTTTAAAATAGTATTGCCTGGGTTTTCTCAGGC
AGTGAGAATCTTTGGATTCTTTCTCCTCCTCTACCTTCTCCAATCTCTGCAGGAGATAAGTCCCCCTTCA
TATCATATCAGTTATACTGGCATTGCAATCACTTCATTTTTTTCTAGCTTTTAGAAAATCCTGTCTCCCA
TTTTCCAGTTCCTCCCTGGCCCTCATTTGTCCACTCTAGCCTCTGGTACCAAAACTCTATTGCCATTTAC
AGCTTTTTCTGACCACAGAAATCTTGGATAGGATCTCAGATGTCTTCATTGGCTAACTCAGACCTCTCAA
TCTTAGAGACTCCAACCCTCCCTCATGTTTGTGTTCACCTGTTCACCTGTTACAGTTTATCTGTGAAATG
TATATTTATATATCTTTCCATCTTGTTTTCTAGGCTGTGTAGCAAGTTCTTCTTGGACAAGGCCTAGCTA
CAATTCTCTAAGCCTTACTTTTTGGTACATAGGGCTATGTATTTAGTACATAGGGCTATGCCCATTTGAG
GGCAACAGAATGAAGCTGTATAAGTTTTCCATTCTCTTCCTGATTGACTTTCCCTTACTAAATCCTGGGC
AGGTCTTGGAAAGAGAGCACACCTGTCGCAGGCCCTGGGCTGTGGTTAATGTATGCATCTGCTTGGCAGG
GCCTGCCAAGCCTAACAGAGAGGACGTCTCCTCGTCCCAGGAAGAAAGTCTGCAGCTGAACAGCATCCCG
CCCACGCCCACCCTCACCTCCACAGCTGTGAAGAGCAGGCAGCCCCTGTGGGGGTTGAAAGAGATGGAGG
AAGAAGATGGCTCAGAGTTGGATTTTGCCCAGGGGTGAGGCCATGGAGGGAATCTGGGTGTCTGAGTTCC
CAGTTTGGTGTCTGGCTGCCTCCATCTTGCTGTTTCTGTTTCATGGTTCCCTGCTGTAGCACTCCCACAT
TGTTGGGTTCTTCTCAAACCCTTCTCCATTGTTTAGCTTTAAAATGCATCTCTCTGAGCTTTTCTCCCCA
TTAACACCAGCTGCCTTACATCCTCTTCTTACCCTTGATTTTTATCCCATCATTGATCCTGCTTCATTCC
CAGCAGTCAGCCCGTCGCAGGCACCGAGAGGTCAAGGTTCTTGGGTCCACAATATTTCCAGACTCCACAC
AACCCTTCAGGTCCTGGCCTGGGCAACCAGCTGATGCGGTGAGCTTTTCTCATCATCTCCTGTCTTCACT
TCAGATCTGTCGCCCACTGTGAGGGGATTCCTGTCTCACCCATTGCCTCTCTGTGGGTGCTTTTTGGACT
TGTTTTAGCCACAGAGCACTTCCTGTCAGCACAATCGGGAAACTACTATGCTGTACTTACTGCTCCCCAC
TTCCCCCACCCCGGGTGTCTGAGAAACTCTCAGGGGTCCTCAAAGAACAGTTTGAAAAGCCAGTCTCTTT
GCCCCTACATCAGACTCAGCCTTATGGAAGAGGACGAGGAAGAAGAGTTAGAAATCCAGGATGAGTCAAA
TGAAGAACGGCAGGATACAGACATGGTGAGTAGACCACCCTGCCCTTCTCTCTCAAGAACTAGGGGCTGA
TGTGCCAAAGAGAAGCCAGGGTGTTTCCCCTCTCTCCGCTCGGTGATGCCTGGTCTGGAAAAGCTGAATG
AATCTAGAGTAAAGGAGGACAGGTTATATGTTGGTTTGGACTTTGCTTGGACTCTACTTTTATTAAAGAA
TAAGGAAAAGAAATTACAAATACCAAAGAAGGTTGGAATAGCACTCTCGCTCCCAATCCCTAAATAAATA
TTGTTGCCTCCGAACCCCCTAGGAATTGGAAGGAGTCCTAATGCTGGAGGTGGGAGGTCATTCAGTCCAA
GTGGCAGCCCCTTCAGTGTCTCTCCCCACAAAACATGACCCCCTATCCTCCATCTCCCTACTAGAGGGCT
TCCCCTCCCTGTCCCTTCCCTCACTCGCTTTCTAACCTAGCCTGTTTCCCCAGGAGGAACTGCTGCTGGT
GTAGTGGGAAAAGCCTGGCATTTGGAGTGAGGTGACATGAGTGCTAGTCCTCAACTCCATGTCAAGTTAG
CGCTTTTACCCCTCCTCAGCCTCCATTTTCTCATCTTTATTTTTTAATTATTTTTATTTTTTGAGGTAGA
GTCTCTCTCTGTCACCTAGGCTGGAGTGCAGTGGCATAGTCTCGGCTCACTGCAACCTCTGCCTCCTGGG
CTCAAGCAATTCTCCTGCCTCAGACTCCCAAGTAGCTGGGATTACAGGCACGCGCCATCATGCCCGGCTA
ATTTTTTTTGTATTTTTAGTAGAGACGGGGTTTTGCCATGTTGGCCAGGCTAATCTCGAACACCTGACCT
CAAGTGGCTCCACCTGGCTCAGCCTCCCTAAGTGCTGGGATTACAGGTGTGAGCCACTGCGTGGGGCCCA
TTTTCTCATCTTTAAAACAGAGCTGTTAGCCTGTAGTTCCAGCAACTCGGGAGGTGGAGACAGGAGGATC
AATAGTCCAGGAGTTGGAGTCCAGCCTGGGTAACATAGTAAACACGTGTCTCTTAAAAAAAAATCAACAA
TTCAAAAATAAATAAAATGGACCTTTTACATAAAATATAAGGTAAAACCACCTTATAAATGGAAAAGGGC
TATGTCTGAGTTCAGGCTACTGTAACAGAATTCCATAGACTGGTGTCTGACAAACAGCAGGTATGTATTT
CTCACAGTTCTGGAGGCTGAAGTCCAAGGGTTGGGTTCTGGTGAGGGCCTTCTTCCAGCCTGTAGACTGC
TGGCTTCTAGTTGTATCCTCACCTGGAGGAGAAACAGCAAGCTAGCCCTCTGGCCTGGGCTTAGAAGGGC
ATTCATCCATCCATGAGGGCTGCACCCCTATGACCCAATCACCTCCCAAAGGCCCCACTTCCAAACAGCA
TCACACTGGGGGAAGGATTTCAATATGTGGGTTTAGGGGGACACAGATATTCAGTCCATTACAGGCTGCG
TATCTTCTGCTGTTATTCTTCTGTTCTCTTCTCTGCAGGGAAGGAAAAAGAACAGAATAGAACAGAACAG
GGGAGTCTTGTGCTCAGAAGGAGAAACCAAACAATGATACCTTCAAACCAGAGACTAGAGAGCAAGGCCT
CTGTTAAAATATTATTTCCTAACATCTCAGAAAAAATCCGTTTTCTGGTTTCCAAAACAAAGTCATTTAA
GGGGCTCAGTCAGCAGGCCAAGAAGCGCTCTGCAGTCAGGCCTGATCCTGGTGCCCACACTGACTTCCCT
CCCAGGAGGTGCCATAGGGGCCTGCTGTGCCCATATTTGTTCTTATGAGGCTGGGAAAGGAACTGGTTTT
TTTATTTGCGAAGTGACAGGAGTGTGTAAAGGCCTTTTTGACTTTTAACCTCATTCTCTCTAGCAAGCAA
GTAGCTACTCTTCCACCAGTGAGCGCGGGCTGGACCTCTTAGATTCTACAGAGCTGGATATTGAGGTGAG
TGTCCCCAGAGCAGGAGTTATGTATCCTTCGGAAATGCTGGCAGGCAACCCGTGCACTCATCAAATTGAC
AGGCCATAGCCTGGGGGTGGCCCTCTGGGCTGTCTCTGGGGCTTTGAGGGTAACCCAGGGGAGGATGGTC
TGCCCATTGGCCCGTTGCTGTGTCCTGTGTATTCCTTTCATCTTTCTCATTATAGGATTTCTGACAGGAC
TCTGGGCCCCTCCCCAGCTCCACTCCCTACCTCAAGAATGTGACCATTTGGAAAAGGCAAAGAGAAAAGG
AGCAAAATGAAGCATTCCCCCAGGCTTCAGCCCTGGGCTCTGAGGGGAAAGAGTTGGGCATTGTTTTTCT
AACCTAACCTTTCCCTCTGGGGTAGAGAAGCCGAGAGACCCTGTCCTCCCTTATGCACTGTGGCCCAGTC
CCCTTGCCTTTTTCCTGTTCTGTTTGGAGTGGAGAAGGGCAGCACCTCTGTGTTTAATGGAAATAGCCCA
TAGTCTCCTGGATTTTTGGAACATCTTTCTCAGCCTATTTTGTGTCCTAATGATTCGCTCAATAAACATG
TTTGAATCCACACGT

Protein Sequence : Show Sequence

>gi|57863310|ref|NP_036579.2| cohesin subunit SA-3 [Homo sapiens]
MSSPLQRAVGDTKRALSASSSSSASLPFDDRDSNHTSEGNGDSLLADEDTDFEDSLNRNVKKRAAKRPPK
TTPVAKHPKKGSRVVHRHSRKQSEPPANDLFNAVKAAKSDMQSLVDEWLDSYKQDQDAGFLELVNFFIQS
CGCKGIVTPEMFKKMSNSEIIQHLTEQFNEDSGDYPLIAPGPSWKKFQGSFCEFVRTLVCQCQYSLLYDG
FPMDDLISLLTGLSDSQVRAFRHTSTLAAMKLMTSLVKVALQLSVHQDNNQRQYEAERNKGPGQRAPERL
ESLLEKRKELQEHQEEIEGMMNALFRGVFVHRYRDVLPEIRAICIEEIGCWMQSYSTSFLTDSYLKYIGW
TLHDKHREVRLKCVKALKGLYGNRDLTTRLELFTSRFKDRMVSMVMDREYDVAVEAVRLLILILKNMEGV
LTDADCESVYPVVYASHRGLASAAGEFLYWKLFYPECEIRMMGGREQRQSPGAQRTFFQLLLSFFVESEL
HDHAAYLVDSLWDCAGARLKDWEGLTSLLLEKDQNLGDVQESTLIEILVSSARQASEGHPPVGRVTGRKG
LTSKERKTQADDRVKLTEHLIPLLPQLLAKFSADAEKVTPLLQLLSCFDLHIYCTGRLEKHLELFLQQLQ
EVVVKHAEPAVLEAGAHALYLLCNPEFTFFSRADFARSQLVDLLTDRFQQELEELLQSSFLDEDEVYNLA
ATLKRLSAFYNTHDLTRWELYEPCCQLLQKAVDTGEVPHQVILPALTLVYFSILWTLTHISKSDASQKQL
SSLRDRMVAFCELCQSCLSDVDTEIQEQAFVLLSDLLLIFSPQMIVGGRDFLRPLVFFPEATLQSELASF
LMDHVFIQPGDLGSGDSQEDHLQIERLHQRRRLLAGFCKLLLYGVLEMDAASDVFKHYNKFYNDYGDIIK
ETLTRARQIDRSHCSRILLLSLKQLYTELLQEHGPQGLNELPAFIEMRDLARRFALSFGPQQLQNRDLVV
MLHKEGIQFSLSELPPAGSSNQPPNLAFLELLSEFSPRLFHQDKQLLLSYLEKCLQHVSQAPGHPWGPVT
TYCHSLSPVENTAETSPQVLPSSKRRRVEGPAKPNREDVSSSQEESLQLNSIPPTPTLTSTAVKSRQPLW
GLKEMEEEDGSELDFAQGQPVAGTERSRFLGPQYFQTPHNPSGPGLGNQLMRLSLMEEDEEEELEIQDES
NEERQDTDMQASSYSSTSERGLDLLDSTELDIEDF

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

38. TBX21

Gene Name : TBX21
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 30009
NCBI Protein GI : 7019549
Genbank Accession : AC015674
Protein Accession : NP_037483
Taxonomy ID : 9606
Chromosome No : 17
Gene Starting Position : 41179682
Gene Ending Position : 41192555
Gene Strand (Orientation) : +
Protein Name : T-box 21
Protein pI : 5.92
Protein Weight : 55747.12
Protein Length : 535
Protein Note : Also known as TBET; T-PET; T-bet; TBLYM

DNA Sequence : Show Sequence

>gi|157713538:41179682-41192555 Homo sapiens chromosome 17, alternate assembly HuRef, whole genome shotgun sequence
GCGGCCCGCTGGAGAGGAAGCCCGAGAGCTGCCGCGCGCCTGCCGGACGAGGGCGTAGAAGCCAGGCGTC
AGAGCCCGGGCTCCGGTGGGGTCCCCCACCCGGCCCTCGGGTCCCCCGCCCCCTGCTCCCTGCCCATCCC
AGCCCACGCGACCCTCTCGCGCGCGGAGGGGCGGGTCCTCGACGGCTACGGGAAGGTGCCAGCCCGCCCC
GGATGGGCATCGTGGAGCCGGGTTGCGGAGACATGCTGACGGGCACCGAGCCGATGCCGGGGAGCGACGA
GGGCCGGGCGCCTGGCGCCGACCCGCAGCACCGCTACTTCTACCCGGAGCCGGGCGCGCAGGACGCGGAC
GAGCGTCGCGGGGGCGGCAGCCTGGGGTCTCCCTACCCGGGGGGCGCCTTGGTGCCCGCCCCGCCGAGCC
GCTTCCTTGGAGCCTACGCCTACCCGCCGCGACCCCAGGCGGCCGGCTTCCCCGGCGCGGGCGAGTCCTT
CCCGCCGCCCGCGGACGCCGAGGGCTACCAGCCGGGCGAGGGCTACGCCGCCCCGGACCCGCGCGCCGGG
CTCTACCCGGGGCCGCGTGAGGACTACGCGCTACCCGCGGGACTGGAGGTGTCGGGGAAACTGAGGGTCG
CGCTCAACAACCACCTGTTGTGGTCCAAGTTTAATCAGCACCAGACAGAGATGATCATCACCAAGCAGGG
ACGGTGAGTGCGGCGCGCCGGCCCTTGGGGCCTCTGTGCCCGCGCCGGAACAAGAACGTCTCGTCTGTTT
TTCTGGCTCGACAATGCTTCTGACTCCGTGTCCCTCACTGCTTTGGCTTCAGCGTAGGGAGACAGGGGAA
TGGGGTTGTTAGGAGGACAGGGAAAGCTCCGGAGGGGCGTCTGTGCCCAGGCTGTTGCACCAACAGCCAG
AGGACTCACAAGGGAGACGGGTGAGTGCGGGACAGTGAGAAGTCACCTTGATTTAGGGGAAGGGTGACTG
TGGCTTCACCTAGAATTGGTGTGCGCCCCTGCCCCACTCTCTACTGTAGAGGAGTCGCAGCGGGCAGTGA
AAGCCTGTGCTCTGGGCGGACAGGACGCCTGGGCCTCCTGTGTGGGGAAACTGGAGGGGAAGGGAGCCCC
TTATCTCCGGGCCCCCTGCGCCCACCTCCCCCGGCTCCTTTGCTGCTGGTGTGCTCAGGTCAGCTTTAAT
GGTGGTAGTGGTGGTGGTAGCGGTGGTGGTGGTGTGTGTGTACGGGGGGAGATTGGGATTTGGTGACATG
GAGAAGCAGTCGCCAAGTTTCCTTTCCGGTCTTACTTTGAGATCATATGTCTGGTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTGTGTGGGTGTGTGTGTATGGGGGCTAGTGCA
GTAAAGCTTGTAGGGGGCACAGATCCCTTCCAGGCACAAATGCCCACGGGCTGGGCAGATGAAGCTCACC
CAGGGGTCCAGCCTGGTAGCCAGCCCCACACTGCACCCTTTGAGGCTGGTCCAGTGAAAACCTTCCCCAC
ACTCCTGTCCAGAAATTCACCGGTTCAGCCTGGAGAAGTGGGGAAGGGGTGTCCCATGGCTTCATGGCTC
AGGGTTCCTGAGCCCCGTGCGTGATGGGGAGAGTTTGGGGCTGAGGGTGCTGCTTCCGGATAGAGCCTCC
TGCGCAAGGAAAGAAACAGAAACCTGTGACTTGTGTGGTATTTGTTTAGTAAGCAACCCCCGGAGTGGAC
TGTGTCTGATGTGGGCTGTGCGCACCCACCCTTCCCAGTGCGGCCCATGTGAGCAGGGGACCAGCGAGGA
CCAGTGTGGAAGGGCTGTTGTCATTGGTGGACCTGGGATGCTGGGTCCCAGGTCCGAGAGGTGTGGATAC
CAAACGTGGGGCTTGGGGTGGAGGGGAGAGGGAGAAGGCCATGTTGGACCCCAGAGGTTGGTATTCGATC
TGGGCATTGCTGGAACATTCTTCCCTCCAGATGATTTTTGTGGGGTAGCCTGGGACTGGGGAACCTGTTG
CCAGCCACAACTGGCTTCAAGTTACTGAGCTGTTCCACTTTCCCTGGGATGAACCCAGGAAAGTTGGCGT
GTTCTTTGATGGCTCAGCCTTCATCTCTTAAGCCTTCTGATAATTTCTCTCTGCTCCCCCCAACCCTAGT
CACCCTTTGGGTGTACCCAGACACAGCCCAAGCTTCTGGTCCCAATGTGTCTGAATTGGAAGGGACCCAG
AAGATCCTGTCCCTCTCACTGCACAGACAGGGAAACAGGCCCAGAGATAGCAAGAGACTCTTCCAAGGTC
ACACAACACACTCATTGCAGGCAGAGCTGGCACTACAGCCCAGATAGTATTTGCTTGGGTTTCTTTCTCT
GGCCCCCTACGGCCACCAGTAAAAACAGCCACCCACGCTCTCTGAAGGCCCTGATGCATTCAGAGTGCTT
TCACTTCCATTATTTCACTGTGCGTTAGGTAGGCCAAGCAGTCTCCCCATTTTACAGTTGAGGTCACTGA
GTTGCAGGGTCAGCTAGGGGCTTGCACTCCATCACACAGCAGACCAGAAGTGGGGCCTCTTAACCTTCCA
CTCTGTTGTGTGGTCAGGAGGACTTCCCACCCTGGGCCCTTGCTGGGGTCTCCCCACAGCCTCTGCCAGG
CCTCTGCCTCCTCCTGCCAGTCTTGAGGGATGGGGTGGATGGAGCATCAGCCAGCACTCTAGGGAGTTGG
CCAGTGCTAGGGGGCTGCCTCTCTGCCTGTAGAGCCAGCTTCAGGGAGGCTGTAGAGCACTTCAGGGAGG
CACTTGAGGGGATGATTCTCGAAGTGTGTATCACCATCTCTACTCCCATGGCCTCCTTTCCTTGGTGTTC
CTGCTGAGTAATTCTCACTTGACAAGTTTTTTTGTCCCCCTTTTAGATACATACACTTACATTTTTTTAT
TATGAAAAATTCCACTACATACAAACATGGACAGAAGAATGTAACAGACTTCCATCCCCAGCTTCCACAG
TTGTCAATATGGGTCCAATCTTGTTTCTGCATCCTCACTTCGCTTCTTCTGTCTGCAACTATATTAGTAA
TATAGTAATTACTAAGTACATTCTAGATATTATTTTATCTCTGCACCTCTAAAAATGACTTTTTAAAATG
AATGCATTACCATTAGCAAACCTGAAAAGAATTTCCTAAATATCACCAAGCTTCTCTGATTGCTTCATAA
ATGTGTGTGTGTGTGTGTTTTCCAATCCTCACACTTTTTGCTCCATTCTGAGGGGCTCCATACGCGGGGT
CCCTGTTGAAGGAGGCAGTGGCTCTTCACTGTTTCTTTTGGTGCTTTTTCTTGGATCAGGCTTCATAGAC
CCCACGCTGCCATGGAGGGGACAGAGGACACACAACCCCAATTCAGTCCCACTAGCCCCAGGTTACCTCC
ATACCTTCTCAGCTTGGGCTCCTGAGATCTGGGAAATCAGTGGCCATTTCCTCTCAAAACAGGCTTTTGG
GCTTATAGCACAGCTTCCTTTCCGTCTTCCTGCTTTTGTGGGCTGGCCTATCCCTCCGTGGGTTCCAGTG
ACCCACCAGTCACACCACATCACCCATGACCTTCTTCCTTTTTGAAAGGTTCTCCCACCTCAGGCCCACC
TACAGCCTACAGCCCCACGGCTTTTTGCCTTGAGCTGACTCACGCCGTCCCGGATTCTTCCTCTCCCACC
CACCCTGGATCTATCCCTCGGTATTGACCAGCGCCAGTGGCATGCTCTTCCCAACGGGCAGAAATGGGGC
AGGGCACAGGGGGTGATTCCTGGGTCTGGAGATTGATCAAGGCAGCTCAGATGGGGCTGAGAGAGGGGAA
CTGAGAACAAAACGGTGGATTATCCCAGTAGGAGACTTTGGTGTCTACGTTTGTGTCTGGAGATTTGGGG
CAGCCTGATAATGCCCCACACAGGCTTGTGCGAGGGTTTCTGTGTGGCAGTGTGTGTGTGTTGCGAATGT
GTTGGCGAGTATATAATAGTTGATGGTAGACATTCTCAGTAGAGGAAGAGAGAGGCCTGTGTCTCTGTGG
GTGTGTGGGTGTGTATTAATTGCTGGCAATCCCCACTTTCTGGGTGTGATTGGTTGATTATTATAATTAA
GATAACTTTGGGTAGCTGTGTTGTGTTAAGCTCAATCTGCATTTGGATTTGTGCATGTGAAGACAGAGGT
TGGTAAAACAACAATTTCAGTCCCACACTATCCTTGCTATCCTCACAGTCATTACAATTTTTTTTTGTCT
CCACTTTCCCAACTCCAAGAACTTTAATTTTTTTATTTGTAATATTTTTCATGGGCCAGGAAGTGGACTT
GAACCTCCCACATAGATAACTTTAGTCGATAACTTTCTACATCTTTTTTTTTTTTTGAGATGGAGTCTCA
CTCTGTCCCCCAGGCTGGAGTGCAGTGGCTCAATCTTGGCTCACTGCAACCTGCACCTCCCAGGTTCAAG
TGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCAGAAGCCACCAAGCCCAGCTAATTTAT
GTATTTTTAGTAGAGACGGGGTTTCGCCATGTTGGCCAGGCTGGTCCCAAACACCTGACTTCAAGTGATC
CCCCGCCTCGGCCTCTCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCTGGCCCAACTTTTCAGAT
CTTAAAAAATTTTTTTTAATTTATTTTTTATGGAGACAGAATATTACTGTGTTGCCCAGGCGGTCTCAAA
TCCCTGATCTCAAGCAATCTCCCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCATTGTGC
CCAGCTAAAAACATTTTTTTTAGGCTTAAGCATGCTTAACTGGTTTCATTTGTCTTGGGGCAAGTGAAAA
CAACTTTAATTAAAAATTTTTTTTAAATGAGACATGGTCTTGCTCTGTCACCCACGCTGGAGTGCAGTGG
TGCGATTATGGCTCACTGCAGCCTCAACCTGCTGGGCTCAAGCAATCCTCCCATCCCAGCCTCCTGAGAA
ACTGGGACTATAGGCGCACATCACCATGTCCGGCTAATTGTTTCTATTTTTTGTAGAGATGGGGTCTCAC
TATGTTGCCCAGGCTGCAGCTTTGCTTTTTGTCTGGCTGTTTGCCAGTGGCTGTCTGAGTCTATTAAGTG
GATCTGAGGTTGGTAAGACGAGGAGTTCCCTTGATGCCCAAGCACATTTTGTACTTTGGTAGGTACGACA
CATTGCCAGAATACCCTCCCAAAAGGTGCTACCAATTTACGCCCACACCAATAGTCTATGAGAGGACCCA
TTTTCTCACAGCCTCATCAGCAGTAGATATTATCACATTTTTTTTTTTTTTGAGACAAAGTCTCGTTCTG
TTGCCCAGGCTGAAGTGCAGTGGCATGATCTCTGCTCACCGCAACCTCCGCCTCCCAGGTTCAAGCGATT
CTCCCACCTCAGCCTCCCAAGTAGCTGGGATTACCGGCATGCACCACCACGCCTGGCTAATTTTTGTATT
TTTAATAGAGATGGGGTTTCACCATGTTACCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCCACCT
GCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCACGCCGGGCCAATATTATCAATTTTTA
AACATTGACCAATATGACTGAGAAGAACTATCTATCTCCTTGCTATTTTAATTTGCACTTAATTACAGTG
AAGCAGAGCCTGTGCCAGGAGCTCCGAATCTGGGAGAGGTATGACGACCCTGTGAACCATCCCAAGGCTA
CTTAGTTCTTAGGGCCAGCAGAGGAATTTGGGGTCTTGGACCCTGTCTTATGAGGGTGGGATGGTAAGTG
GCCTTTAGGGACGCTCAATTTGACACCAGACCCATCACCACTCTCGGTTCCTTCCCAGCAAAGTGTCATG
TAAATCAGGGGCTATTTTGGGATTCCAGCTGGTGGTGATTCTAGACTTTTAGGGAGAAACCGAGATCTCC
TTTCTCTTCTCCCTTTTCCCCAGCCTCTTCCTGTGTCTCCATTTCCCTCTACTAGTGATAAAAACAGGAT
GAGCTGGGCATGGTGGTGAGCACCTATAACCCCGCTCGGGTGACTCGGGAGGCCAAGGCAGGAGGATTGC
CTGTGCCCAGGCATTTGAGCAGAGCCTGTGCAGCATAGCAGACTCCATCTCTTAAAAAAAAATCAGTATG
GCCTGGGCGCGGTGGCTCACCCCTGTAATCCCAGCACTTTGGGAGGTCGAGGCCAGTGGATCACCTGAGG
TCAGGAGTTCGAGACCAGCTTGATTAACATGGTGAAACCCCGTCTCTACTAAAAGTACAAAAATTAGCCA
GGTGTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATAATGGCGTGAACCT
TGGAGGCGGAGCTTGCAGTGAGCCAAGATCACACCACTGCACTACAGCCTGGGCAACAGAGCGAGACTCC
GTCTCAAAAAAAAAAAAAAAATTAGCCAGGCGCAGTGTTGGGTGCCTGTAGTCAAGGAGAATCTCTTGAA
CCTGGGAGGCAGAGGTTGCAGTGAGCCGAGATCACACCACTGCACTCCAGCCTGGGGGACAGAGCGAGAC
TCTGTCTAAAAAAAAAGAAAGAAAAAAATGAATATGAAGAAATGGGGACCACAGAGCACAGCATGCACTC
TGCAGTCAGAAGACCTCACCTCTGCTCTCCCACCGAGGACCGTCACCAAACCTCTCGGAGCGTCAGTTTC
TTCAAATATAAAATTGGAAATAACATTTTATTTTTTATTTTTTCTTTGAGATGGAGTCTCACACTGTTGC
CCAGGCTGGAGTGCAGTGGCGCCATCTCGGGTCACCGCAACCTTCGCCTCCCAGGTTCAAGCGATTCTCC
TTGCCTCAGCCTCCAAAGTAGCTGGGACTACAGGTGCATGCCACCACACCAGGCTAATTTTCTGTATTTT
TAGTAGATATGGGGTTTCACTATGTTGGCCAGGCTGGTCTCGAACTCCTGAGCTCAGGAAATCCACCCTC
CTCGGCCTCACAAAGTGCTGGGATTACGAGTGTGAGCCACTGTGCTGGGCCATCTTTCGAACTTTTTAAC
AGCTTTCAGAGGAAAGATGGACAGGAGTTAGACTCTCCATTTACATGTGAAGAAACTGAGATTTGGGCTG
GGTGCAGTGGCCCATGTCTATAATCCCAGCACTTTGGAAACCTGAGGCGGGTAGACCACTTGAGGTCAGG
AGTTCGAGACCAGCTTGGCCAACGTGAAACTCCATCTCTACTAAAAATACAAAAATTAGCCGGGTATGGT
GGCACATGCCTGTAGTCCCAGCTCTTGGGAGGCTGAGGCAGGAGAATCACTTGAACCTGGAAGGTGGAGG
TTAGAGTGAGCTGAGATCACGCCACTGCACTCCAGCCTGCATGACAGAGCGAAACTCCATTTCAAAAAGA
AACTGAGATTTGGCTTGAGAATTAACTTATCCAGGGTCATAGGGTAGTAATCTATTTTTCTCAACTCAGG
AAAGTCAGGGGAAGACAGGCGTGTGACAGCACCCTTCTAGGACACCTTCCCAAGGTTGTTAGGTAAGTTG
TCCTGGGGACCTCTGGGGATTAGAAGTCCCTCAAAAACAGTTCCTAGGCCCCTGGAACCCTCCCTCCTCC
AGGTTTCCTGACTCAGATAGGTCCCTTTTTCCTCATCCTTCAGGGCTTCTCATGAGAGGAGGGGGAAGTG
TGTGGAAATGAAAAATAATTCTGATGAGGATTAGCTTCTGCCATTCTGAAAACTCTGCTCTTCCTGCTTG
TGTTAGAAGAGAAAGGCTAAGCCATTGGAGCTGGTGATGGAATTGGTGCTGGTGGAGGTGGTGGTTGTAG
TGACGGTACTACTGGTGGTGGTTGTGATGGTGAAGGTGGTGGCTGTAGATGGTGGTGCCCGTGCTGGTGC
TGGTGGAGATCGTGGTTATCGTGATGGTGGAGGGGGCAGTTGTAGTGACGGTGGTTGTGATGGTGGTGAT
GGGTGGTTACAGTGCTACTGGTGGTGATGGTGCTGGTTGTAGTGGTGGTGGTGACGGGGCTAGTGAAGGT
GTTAGTGGTGGAGATGGTGATGGTAGAAGAGTGCTTCTTTTCTGAATCTGTTACTCTGGCACAACAGCGG
AATCATACAGCATCCCACCCCCTAACTTCCTCTACTTTTCCTGGAAACAAGGACTTTGAACATGAGCCCC
GTGTTCAATAGAGCCTTCTTTCAGGTGGATGGGAAGCTCCCTGTTCAACTAGGATGTGTGCAACTTCTCT
AAAGGCTGAGGGCAGACTATACCGCAGGTGGCAGACCCAGGAGCATGGGAGAGGGAGGGCCTGTGAGTGG
GTGTCATGGGTCTGGGAGTGTGTTAGCACTGTGAGAGCCACGGCAGAGAGGGTGGCTGAGGAACTGAGGC
GTGAGGACCTTCCAGGAACTGCCTGTGGAGAGCTGGTGGTGCCCCCTCCTAGGAAGCAGCTAAGGTTAGA
GACAGGGGTGTGCAGGTAGATGTCGAGCAACTGACCCTCTGAAAGAACATTTTAAAAAACTCTGATTTGT
AGTGTTTGCTGATCCCTGTGGTGTAAATACTCCTGCTATGGATGATTTCAAGCGACTCATGTGAGTCATT
GAACACAGAGCCGGGAAGAGACTCACAGTGCACTGTGATTTAGTACCTCCTCCATGCAGCTGCGATAGAC
GGAAGTAACCTCGACAGTGCAGACAATAGTAAAACGTAAAATAACTGGGCAGTGATGATTTTTTAGTTTT
TACTCCTTTTTTTTTTTTTTGAGATGGAGACTTGCTCTTGTCATCCAGGCTGGAGGGCAGTGGCGCGATC
TCAGCTCACTGTAACCTCTGCCTCCTGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTAGGA
TTACAGGCGCCCACGACCACACCCAGCTATTTTTGTTGTTGTTGTTGTACTTTTTAGTAGAGACGGGGTT
TCGCCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGCCCGCCTTGGCCTCTCAAAGT
GCTGGGATTACAGGCATGAGCCACTGTGCCGGGCCTACTTTGACTGTTAATATGTCTTGAATTGCAAGTT
TATATAATTTAATTTTTAATTATGGCTGTGTTTAACCACTGGCTGGCAAACTCCCTAAACACCTTCCAGC
TGGTTCTTGTGAGTGGGAGGAAGCCGGCTACAGCACACCACTGATGCCTGGGCACTGTTGCAGGGGGGAC
TGGCTGTCAAGCTGGAGCTGATGGGTGCTGGGGGCTTTCTCTCTTCTCTCCAGGCGGATGTTCCCATTCC
TGTCATTTACTGTGGCCGGGCTGGAGCCCACCAGCCACTACAGGATGTTTGTGGACGTGGTCTTGGTGGA
CCAGCACCACTGGCGGTACCAGAGCGGCAAGTGGGTGCAGTGTGGAAAGGCCGAGGGCAGCATGCCAGGT
GCGCGCGCCCCTGGGAGCGGTGGGCTCTGTTTCGCTGGGACTGGGCGCCCCCTGGTGGGCCCACCAAGCC
CCTACCCCTAATTCCTAGACCTTTAACCCCCTCCCACTCCATCCCACGCCATTGCATCCCTCCTGTTTCT
GGCTTCCTGCTTTGCTCTAGCCTGTCCTCTGCTGAGTCCTCTGCCCTTCCCTGCCTGGTCCTCCCCCTGT
GTCCTTCCTTACGTCCCTCTCGGGACAGGCAAAGCCCTACATCACCAGGGTTCTGTCCCGGGGGCTGCAT
GTCAAAGAGGTGAACTGTCCACAGGAAACCGCCTGTACGTCCACCCGGACTCCCCCAACACAGGAGCGCA
CTGGATGCGCCAGGAAGTTTCATTTGGGAAACTAAAGCTCACAAACAACAAGGGGGCGTCCAACAATGTG
ACCCAGGTAGGACCTGCTCTTCAAAAGGTAGCCTCGCCCTGCTCCCCACCCTGGGTCTGAGACCTCCAAG
GCCACAAGGGTCCTGCGGGGCCAGTTTGGTTCATTTTTTCTTCCTTCCTACAGGAAGGAAGGTTCGTTTT
TCTTCTGTCCTAAACGAAGGGTCATTTTTCCTCCTTCCTAAACTCTGGCTGTTTCTCACCCACCTTGGGA
GGAAGATGAGATGGGATGAAGCTGAATCTTGGACGGGGGTCATATTCAGGCCACACAGGCCAACCCAGCC
TCAGGGTTTCTTCACCAAATCATGGGTTGCCATTGCTCCGGCACCATGAAAGGCACAGAGTTGGCCCTGT
GGTCCCAGGGAATAGATGCCGAGTTTCTCTAGGTTGAACAATCCTGTTAAAAGTGCCCTTGCTGGCTGGG
TGTGGTGGCTCACACCCATAATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATCACAAGGTCAGGAGTT
CAAGACCAGCCTGGCCAAGATGATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAAGCATGGTG
GCGGGTGCCTGTAATCCCAGCTACTCAAGAGGCGGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGG
TTACAGTGAGCTGAGATCATGCCATTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCGTCTCAAAAAAA
AAAAAAAAAGAAAAAAGAAAAAAGAAAAAAAAGTGCCCTTGCCCTAAAGCCCTGTTTGTGCTGATACCTT
GGTCTCAATTGACATCTGGAGGTAGAAGAGGGGGCAAGAGGCTAGAATTGGTGCCCCAAAGAGTCCGGAC
ACTGGAGTTGGAAAGCTTGGGGGAAGTTACATGGTGGCAGAGCAGGGGAATGGACAGGATGACCTCAAGG
TGCTTGCTAGCCTCACGTGGGGCCAGCTGTTGGTGGGGGTGATGGGATCCTCGAAATCCTTCCTCCCCAC
CCCTACAACCGTCTTGCTCTGTCTACAGATGATTGTGCTCCCAGTCCCTCCATAAGTACCAGCCCCGGCT
GCATATCGTTGAGGTGAACGACGGAGAAGCCAGAGGCAGCCTGCAACGCTTCCAACACGCATATCTTTAC
TTTCCAAGAAACCCAGTTCATTGCCGTGACTGCCTACCAGAATGCCGAGGTGAGGGCTGCCTGAGCCCCG
GTGGGGAGGAGGGCAGAGTGGGGCCCACTGTCTTCCTTGGGAGGGATTTGGAAAGTTCCCGAGCCCCAGA
CTCAGGACTCAGGTGACTCTATTTCCCTTCTCTCTAGATTACTCAGCTGAAAATTGATAATAACCCCTTT
GCCAAAGGATTCCGGGAGAACTTTGAGTCGTAAGTGCCACTGGGTTCAACTCAGCTTTGGTCCCTCCTGA
GACACATCCTCTCCCTGCCCCTGAAAACAGGAGGGTGGGGGACAGATGCTACAGGTGGGCAGGCCAGGGA
AGGAGGGTCGGAGAAGGAATGTGTGAAACAGGTAGGCTCACAGGTGACTGGTTCTGCTTGTGACCCGTTT
TCTTGCCTTCTATTTTTTTCTAGCATGTACACATCTGTTGACACCAGCATCCCCTCCCCGCCTGGACCCA
ACTGTCAATTCCTTGGGGGAGATCACTACTCTCCTCTCCTACCCAACCAGTATCCTGTTCCCAGCCGCTT
CTACCCCGACCTTCCTGGCCAGGCGAAGGATGTGGTTCCCCAGGCTTACTGGCTGGGGGCCCCCCGGGAC
CACAGCTATGAGGCTGAGTTTCGAGCAGTCAGCATGAAGCCTGCATTCTTGCCCTCTGCCCCTGGGCCCA
CCATGTCCTACTACCGAGGCCAGGAGGTCCTGGCACCTGGAGCTGGCTGGCCTGTGGCACCCCAGTACCC
TCCCAAGATGGGCCCGGCCAGCTGGTTCCGCCCTATGCGGACTCTGCCCATGGAACCCGGCCCTGGAGGC
TCAGAGGGACGGGGACCAGAGGACCAGGGTCCCCCCTTGGTGTGGACTGAGATTGCCCCCATCCGGCCGG
AATCCAGTGATTCAGGACTGGGCGAAGGAGACTCTAAGAGGAGGCGCGTGTCCCCCTATCCTTCCAGTGG
TGACAGCTCCTCCCCTGCTGGGGCCCCTTCTCCTTTTGATAAGGAAGCTGAAGGACAGTTTTATAACTAT
TTTCCCAACTGAGCAGATGACATGATGAAAGGAACAGAAACAGTGTTATTAGGTTGGAGGACACCGACTA
ATTTGGGAAACGGATGAAGGACTGAGAAGGCCCCCGCTCCCTCTGGCCCTTCTCTGTTTAGTAGTTGGTT
GGGGAAGTGGGGCTCAAGAAGGATTTTGGGGTTCACCAGACGCTTCCTGGCCCACGATGAAACCTGAGAG
GGGTGTCCCCTTGCCCCATCCTCTGCCCTAACTACAGTCGTTTACCTGGTGCTGCGTCTTGCTTTTGGTT
TCCAGCTGGAGAAAAGAAGACAAGAAAGTCTTGGGCATGAAGGAGCTTTTTGCATCTAGTGGGTGGGAGG
GGTCAGGTGTGGGACATGGGAGCAGGAGACTCCACTTTCTTCCTTTGTACAGTAACTTTCAACCTTTTCG
TTGGCATGTGTGTTAATCCCTGATCCAAAAAGAACAAATACACGTATGTTATAACCATCAGCCCGCCAGG
GTCAGGGAAAGGACTCACCTGACTTTGGACAGCTGGCCTGGGCTCCCCCTGCTCAAACACAGTGGGGATC
AGAGAAAAGGGGCTGGAAAGGGGGGAATGGCCCACATCTCAAGAAGCAAGATATTGTTTGTGGTGGTTGT
GTGTGGGTGTGTGTTTTTTCTTTTTCTTTCTTTTTATTTTTTTTGAATGGGGGAGGCTATTTATTGTACT
GAGAGTGGTGTCTGGATATATTCCTTTTGTCTTCATCACTTTCTGAAAATAAACATAAAACTGT

Protein Sequence : Show Sequence

>gi|7019549|ref|NP_037483.1| T-box transcription factor TBX21 [Homo sapiens]
MGIVEPGCGDMLTGTEPMPGSDEGRAPGADPQHRYFYPEPGAQDADERRGGGSLGSPYPGGALVPAPPSR
FLGAYAYPPRPQAAGFPGAGESFPPPADAEGYQPGEGYAAPDPRAGLYPGPREDYALPAGLEVSGKLRVA
LNNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKA
EGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEP
EAACNASNTHIFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENFESMYTSVDTSIPSPPGPNCQFL
GGDHYSPLLPNQYPVPSRFYPDLPGQAKDVVPQAYWLGAPRDHSYEAEFRAVSMKPAFLPSAPGPTMSYY
RGQEVLAPGAGWPVAPQYPPKMGPASWFRPMRTLPMEPGPGGSEGRGPEDQGPPLVWTEIAPIRPESSDS
GLGEGDSKRRRVSPYPSSGDSSSPAGAPSPFDKEAEGQFYNYFPN

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

39. TLR1

Gene Name : TLR1
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 7096
NCBI Protein GI : 41350337
Genbank Accession : AC108044
Protein Accession : NP_003254
Taxonomy ID : 9606
Chromosome No : 4
Gene Starting Position : 38797875
Gene Ending Position : 38806411
Gene Strand (Orientation) : -
Protein Name : toll-like receptor 1
Protein pI : 7.07
Protein Weight : 86022.3
Protein Length : 786
Protein Note : Also known as TIL; CD281; rsc786; TIL. LPRS5

DNA Sequence : Show Sequence

>gi|224589816:38797875-38806411 Homo sapiens chromosome 4, GRCh37.p5 Primary Assembly
CTTATGTGTCAGAACTGTTTAAATCAAAGTTATATCATTTCATTAATTTTTAATACCACATATAAATGGT
GAACTGCGACCCGAAGGTATATATTTTTACCTACATCATACACTCACAATTGTGTTTACATCTATGCTGA
TGCAAAATAAAGTCATTGTTGGAACTTCCAAAAGCAGCAATATCAACAGGAGGAATATTTTTCACTTGAT
GTGTAATCTATTTCTTTGCTTGCTCTGTCAGCTTAATATTAATGGCTGCCCTTAAGTTAGCCCAAAAAAG
GCCACGTTTGCTCTTTTCCTTGGGCCATTCCAAATAAGTCCTCCTGGCCATGAGACTTTTGAGCTTGTGA
TAACTGCTAGGAATGGAGTACTGCGGAATGGGTTCCAGCAAGATCAGGATTAAGCTATTAGATCCTTCAT
GAAAGAGATTGTGATGGGCAAAGTAGAGTTCATAATGGCACCATTCACTCTGGACAAAGTTGGGAGACAA
AACAAAGATGGACTTGTAACTCTTCTCAATGCAGGTGATGATATTTTCCACAATGCTCTTGCCAGGAACA
AAGTTTCTCTCATGAAGGCAAATCTGCATACCTTCTTTCTCTAGGTTTGGCAATAATTCATTCTTCACCC
AGAAAGAATCGTGCCCACTATATGAAATAAATGCATGAAACTGGAGATTTCTTTGGAGTTCTTCTAAGGG
TATGTTCCTGGCCCTGCGCCGGGTCTGGGTCCACTGGCACACCATCCTGAGATACCAGGGCAGATCCAAG
TAGCTGCAGAGGGAGGTCACAGTCACAGCCAACACCAGCATGGTGGCAACGATGGTGACGATCAGCAGAG
TTATGTTGCAGGATAATTCAGACATGTGAAAGTCCTTTAGTAGGGTTCCTCTATAACTTTCCGGGTAGTC
ACACTTATAAGAATCAGGCCAGCCCTCTAACACTTCACTTGATACTTGGTCTATATTTTTGACAAATTCT
CCTAGCTCACAGGTACATTGGAATGGATTGTCCCCTGCTTTTATTGACCTCATCTTCTGGCAGCTCTGGA
AGAAATCAGCCGATGGGTGGGAAACTGAATTGTGATCAATGATCAATACAGAAAGGCTGCTAAAGCTGCC
ACATCCAGGAAGGTCAGTTAAAGAATTGAAAGCAACATTGAGTTCTTGCAAAGCTTCCAGTTTTACGACT
TGTTTAGGAATGCTCTTTATTTTATTGCTGTGAAGATCAAGTACCTTGATCCTGGGAGGTAAACATCTGA
AAATAGTGTCAGTAAGTATATTTGAAGACATATTTAAACTTAATAAACTTTTAGTCCAAGAACAGTCTCC
TTTCTTTTCATCATAGCTTACAGAATTCTGGCTAATATCCAATTGTTGCAGAGACTTCATCTGTGTAGTC
ATTTCAGCTATTTTTGAAAGTTCTTTTAATTGATTCATTTGTAAAATAAGTGTCTCCAACTCAGTAAGGT
GCCCACAATTTTCAAAAACCGTGTCTGTTAAGAGATTATTGGAAAAATCCAAATGCAGGAACGGGCTAAT
TTTGGATGGGCAAAGCATGTGGACCATGCGTGTACCAGACACTGTGAAATTTTTGATGTTCATATTCGAA
AAGATTTCATAGATATAACTTTGCGGAAAACCGAACACATCGCTGACAACTTGGTGTATAGACAAGGCCT
TCAAGGAAGTGCCAGAATAATCAAAATCTCTGAAGTCCAGCTGACCCTGTAGCTTCACGTTTGAAATTGA
GAAATACCATACAGTTGTATGCCAAACCAGCTGGAGGATCCTAATGAAAGAATTCCAAGTTGTTTCAATG
TTGTTTAAGGTAAGATTTGATAACTTTGGATTTGTTTGAAGTTTCGCCAGAATACTTAGGAAGTAAGAAC
ATTTGTTATCTTCTAGCACACATTTGATATTAGATAGTTCCAGATTTGCTACAGTCTTGACTGACACATC
CAAAATAAAATGGAATTCTTTGTTTGTGGGGAACACAATGTGCAGACTCTCAGTGTTAAAGTCTTGAAGG
CCCTCAGGGTCTTCTTTTTCCCCATAAGTCTCTCCTAAGACCAGCAAGACCTTGCTGATATTCAAATGAG
CAATTGGCAGCACACTAGATTTTTCTAAGTGTGTGGTGCTCAACCCCAGAAATTTTAGTTGAGACATATT
GCCAAACTCTTTGCATATAGGCAGGGCATCAAATGCATTAAATGACAGGTCCAAGTGCTTGAGGTTCACA
GTAGGGTGGCAAGAAATCTTCACCAACTTGTTGTGGGACAAATCCAAGTATTCCAATTCCTGGTTGAATT
TGAAAACACTGATATCAAGATACTGGATTCTATTATGAGAAATTATCAAAATCCTCAGTTTTGACAGTGA
TAAGATGTCAGAAGTCCAAAGCTCAGATATATAATTTTGCGATATATTTAAGATTGTTGTTTTCTGGGAT
AGGTCTTTAGGAACGTGGATGAGACCGTTTTTTGACCTATCAACTAAAAATTCACTTTCTTCAGATAATT
GTATTCTGATCTGAAGTATTAACATGAAGATAATGGCAAAATGGAAGATGCTAGTCATTTTGGAACACTA
GACATTCCTAAAGGTAGAAGCTGTTCTTCAGATCATCTTGATACAGATACAGATTCTAGAAAAAAAATAA
TGAAATGATGAAATACATTACATACATTTTTAAAATACACGAAATTAGTCATGGCTGACATTAAACTTTT
TTTTGTTACAGACTTATAAAGTGGAGGCTACATTCTTTTGGGTTACATGGCCTAAAACTAGAGAAGTATA
TATATTGGTAAATAATGATGCCAGCTCTAATCAAATAAGTTTTAATATGGATGGTGTAATAGGTTGAATG
GTGGCTCACCAAAAGATATGTCTACATCTTAACCATGTCCTAATCCCCAGAAACCATGAATGTTACCTTC
CTTAGAAAAGGGGTCTTTGCAGAGATAATAAAGTTAAGGATCTTGAGATGAGGAGATCATCCCAAATTAT
CCAGGTGGTCCATAAATCCAATGACAAGTGCTTTTTTAAGAGAAAGGCAGAGGGATATTTGACAAAGAGA
CAAGAGAAGAGGCAATTGAGACACAGAGGAGAAGGCCTTGTGACAGTGGAGGCAGAGACTGGAGTGCAGC
AGCTACAAGGCAAGGAACATCAACAGGCGCCAGAAGCTAGAGGAGGCTCTGGAGTGATGGTGGCCCTGCT
GACACCTTGAGTTTCGACTTCTGCTCTCCAGAACGTTGAGGGCAAAGCCACCACGTTTATGATCATCTGT
TACAGCAGCCTCAGGAAACTAATACAGGTGGGTTCATAGAAAGTTTTCTGGCCCCAAGCTTCCCAAAATT
ATTTAGAACTCCAGCTAAGACTCAACTGGTGTACTTTCACTCTTTGACGTACATCTCCCCATGACTTTCT
CAAGAGGTGTTCCTAATGGTGGTATTGGGACTGCTCTTCTATTACCCTTTATGACCTATACACGGTTTGG
GTCTAACTTTGGTCTTACTCAAAAGGTAATTAGATCCTGATAAAAGGCCTCTCAGAAGATCCATTTATTC
ATCCATTTATTTATTCAACCAGTATTTATTGATCACCTACTGGCCATGTTTTAAGAACTGGAGATAGAGC
AGTGAACAAGATAAAGTCCGAATTCTCAAGGAGTTTTTATCTAGTGGGGAGACAGAAAATAAATGAATGA
CTAAACTCAAGTTCCTGTAGTGATAAGTTCTGTAGAGAAAAATAAAGCAGGGTAAATTGTAGGTATGAGA
CAGTGCTTGGGGGTGGCTATTTTTGAGTGATTAGTTAGAGAAGGCCTTTTTGAGATGATGACACTAGAGT
AGAAATCAAAATGAAGTGAATGAGTAACCTATGGGAAGATCTGGGTAACCTACAGCATTTCTGGCAAAAG
AAAAGGCAAGTATAAAGGACCTGAGATGGAATTGAGCTTGGCTTGTCTGAGAAGGAACGGGAAGGTCAAT
GTTAGTAAGGAGTTAGGGAAGATGAGAGGAGTTAGGGCCAAGATCACTCAGGCTTTGTTGATCATTGCAA
GGAGTTGGGATTCTATTCCAAAAATGATGAGAGGGTGGAGAACACTGGGAGGTTTTGAGAAAGGGAGTTG
TTTCCTCCTCTCTCTTATTCAGAATACTTTTTTCATTCATTCATCAAATATGTATTTATTCTCCATGCAG
TGGTAGGCACTGTTCTAGGTTATGCCTATTGTACCAGTATTCAAACTGCTCTAGCTAAGACACCATTTTG
TGGTCAGCATTCCAGTGGCGTATGAGAACAGAGGGAGCTGTGTTGGGATGAACTCTAGAGTGTGCAGTCC
TCACCTCCAGAGACCCTTTCAGCCGTGATTACACAGCTACCCAGTTCTCACAAGGAACTTATTCAAAATC
CCCTAGAACCATTAGACAAGCAGGGCATTAGTATCAGCAAAGCACCAAATTCCTCTTCACATATCCCCTC
CCATATCTCAATGAGCACAGAGAACCTAAATAATTTAGCTGCTACAAACAACCCTCGGTTACAAAAGTTC
CCCAATTTAGATGTGCAAACTTATCTGGGTTTTGGAGCCTTCCTTGGACTTACCCTTTTGTAGGGGTGCC
CAATATGCCTTTGTTATCCTGATTTCTTCTAACGAGGAAGAGGGCCTGGTACCCCTATTAGTGTTCATGA
AGACCCTGAGCAAGGAAGATGATGCAGACATTAATGTTCATGTTAAAAGTCAAGGTGGTGTTTTTATGAC
ATTAACTGTATTTGGCTGAAAGACCCGGAAATTTCCCAATTCTTCCTCTCCAGCTTCCTTAAAGAGCTAC
TCACACAAGGAGCAATATTTCAATGGATGTTATAGCTTGAATGTTTGTGCCCTGTGAAATTCTTATGTTG
AAATCCTAACCCCCAAGGTGATGGTATTAGCAGGAGAAAATTGTGAAGTAATTAGGTCATAAGAATAGAG
CCTTTATGAGTCAGATTAGTACCTTTATAAAAAAGACCCCAGAGAGCCTCAGGCTGTCATTCTGCCATAT
GAAAATACAAGAAGGAGGCATCCGCAAACCAGAAGAGGGCCTTCGCCAGAACCCAACACTACCACCCTGA
TCTTAGACTTCTACCTTCCAGAACTGTGAAAAGTAAGTGTCTGTTGTTTATAAGTCACCCATTCTGGTAC
TTTGTTACAGCAATTTAAACAGACTAAAATAATGGGAATAAAAATAATACCCTGGGGCAGTGCCAAGCTA
GGTCGAAGCAAAAGTGGCCTGGTCTTAAGGCCTAAGTGGGGTTCCCTGCTTATTCCTATGCCTGAATCCA
GATCAAGGCTCTTTCCACCTCCTAGCCAACTCCAGAGTTCATGGAAGCAGTGATCAGCACAGTCAGGCGT
GGTATTCAAAAGTGATCAGAGGCACACAGGACCCCGTGCATGAGTGCGAGAGCTTGGCTCGCAGAACCTA
CCACACGCAAGATGAAAGTTGTCCTTTTTCCCTTAACAACAGCTATCATCACAAAAATATATAAATTTAT
ATAACTGCCTGCCCCCAAGTGTTAGAGTAGGTACTTAGGCAGACATGAGCAGGACAGGAGAGGGGCCCCC
CAACCAGGAATATCAGGTGACCATCAGGTGATGATCAGGCAGTTGTTAAACAGTCTCTCTAAGCCGGGGG
CCATGGCTCACGCCTGTAATTCCAGCACTTCGGGAGGCTGAGATAGGTGGATCACTTGAGGTCGGGAGTT
TGAGACTAGTCTGGCCAGCATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGATG
GCAGGTGCCTGTAATCCCAGCTACTGGGGAAGCTGAGGCAGGAGAATTGCTTGAACCCAGGAGGCAGAGG
TTGCAGTGAGCCAAGATCATGCCATTGCACTCCAGCCTGGGCAACAGGGCTAGACTCTGTCTCAAAAACA
AACAAACAAAAACAAAAAAACAGTCTCTCTAGAATAATAATTGTTTGCAGCCAGCATCAGGGAAAGTCAG
TCTCCTAATAGATAGAAAACACACCTGAAGCTGGTGGTTAGCAGACTCCTGACAAACTCTCAGGAGCTGG
GCAAGCAGGCTTGAGCATGCATACCAAGAGGCAAAATGGCGGACTTCAACGGGTATGTGACCTTCCTCTA
GGAAGACTTGTCTGGCAAGGGAAAAATGTCTCCAATGAGCATGTACACAACTTCAGTAAACACAGTGTGT
GTGCAGCCCATCCCAAGGGCTGGCAGGCCACTGCACATGCAGACAGCCCACCCCAAGGGAAGAATCAGGG
GAGAAGAGACGCAAGACTCCAGAAGCCTGCCAACAAATAAGCCCCAAGTAATAGGTTAAACTGGGCACTT
GGATCTCTCAAGTTGCCCACTTGGCCCTCCTCCAAGTGTACTTTATTTCCTTTCGTTCCTGATCTAAAAC
TTCTTAATAAACTCTCTGTAAGGAATATGGCTGTGCTTTGGTCAAGGATAGGCCAAGGTAAACATCCAGA
GTGACTCAGCGAGTTTAGAGCGCAGGCGTATAACTCACTTTTTATCATAGCCATGTAGACATAACATAGA
GAAGCTTACCATAGCCATAACATAGGGAAGGTTCATCCCTTGGCTCTAAGCCACTATTGTCCGTAAATGG
TATAATTGCCCTGCTGACGCTGTACAGGTGCATTGGCAGCCAGAGAAAGAGAGAGAGAGAGAGCCAAAGC
TGTCCATCTTTGCAGATGGACTGAGGGGAGCCAGGACACAGCTCGGCTTGCTTGTACCCAGAGAGAGAAA
GAGTTAAGCTGCTGACCCTGAGGGCAAGGGAGAGCCGGACGCACAGTTGTGTGTGGAAGCTGCCGAACTA
AGCAGCCAGGACAAGGCGGACAGTCTGAGAGAACTACTGTAAGTAAGCTGCTAATGAGAGCTGTTGCTGA
ATAAAATCACCTTTCACCTGCCTACGGCCCCCTGAGTGTTCTTTCAGCTATCTGCTCATCCACCCCCTCC
CCTCAGACCTCAGTATGGGCTGGAACCTGGCCCTGGGCGTGACACTCTCTCTCTTACTCTAAAACTTGCC
TCGGTCTCTCACTCTGCCTTATGCCCCTCACACAAATTCTTTCCACTGAGGAGGCAAGAATTAAGTTTCT
ACGACCCATATGGATTCACCACTGCTAACGCAAGCATTTAATGAAAGCATAGCTTCAGTTTTATGTCGAG
CTAAATTAATTGACTCAAATATCTTCTATTTGCCTAACCTCCATCTTGCCCTTTCATTTAAAGTCATTAA
TTTTAAGTACTCAGTTTATGAAACCATTCTTATTTCTATAATTAAGAAATCAAAATGTTATACACAGGAA
TAACTGATTTTTAATACTTCCAGGATCTTTGAATTTTATATACCAAACTTGCTAAATCCATGAAAATCAA
TGTCTCAGTTTCCTAGACCAAAGAATTTTTTGATCAACGTCTTAATGACCATGACTGAGCCTCATCTATT
TTAGAACCTTTAAACTCTTTTACTATCCAAAAAATAAGCTGAGCAGAAAAGGATGCTTTAATTTGAAAGT
GTAAAATGCCTCTAGGTGAGCTTCTGTGAATTTTCATTATTATGATAGGTTTAGCCTCACATTATATGAT
ACAATGAATCAATACATTTAATTTGTCTTCTTTTGGAGACTTTGGAGGCATCAAATCAAGGCTTTGACTG
GTCTTAATGGACAATACCCATACAATCTATCTGGCCCATTAAGTTTAGTCCTGTAAATATTTACTAAGGG
CCTTCTCTGCACAAGACCAAATAGCTATTAACCAAAGGTTAAAAGAATCTGAGACAGTTCAAGAACTGTT
CTGCTTTGCTTTCTATGCGGTTTTGTGAACTCTTGCTTTGTTATAAAACAAATTCACTTTCAAGTCTTTT
GACCAAATTGGATGTAGCCTGGGAAACATTTTCAAAACCTGCAATTCTACCTCACGTCTAGCTCCCCAGA
ACAGTGTTAGTGGCCTGAGAAACAGAAGGACTAGCTAGTGGGAAGGCCCCAGAGAGAAAAACTGAAACAA
ACCTGGCCACAAAAACAGAAGAGCTGAACAGCAGCATTGCCTCCGGGAGTAACTGACTTATATCTCATTT
TCTTTAACACTGAGAAGAGAATACAAATGGCGGGATTAGGTGAAGAGAAATGCTGTCAAAATCAACAACT
AGCAGCTGCTCTGGAAGAAAACCAGAAGACGTGTGTGACCCAAATATTCATTTCCCAACTCAAAAATATT
TCCTATAAGCAAATCACACACGAGGTGTTATTCCCAGAGGAGTGTGGGGACTTTCTCTGATTTAGGACTC
AGAACTGATACACAAATGCCAGTACAAACTACACAGTAACTATATACATATCAGAAATATAACCCACTTA
GAAAAACACCCAGGACTTACACAGATTTTTGAGACTTCTGTACTTAATAACTAGCTATACAATTATTTTC
TTTTACAAAAGAAAAGAATGTGTTGCTTACCAAGACAACCTGCTTGTCTGTTCCATTTGGCAGTCTG

Protein Sequence : Show Sequence

>gi|41350337|ref|NP_003254.2| toll-like receptor 1 precursor [Homo sapiens]
MTSIFHFAIIFMLILQIRIQLSEESEFLVDRSKNGLIHVPKDLSQKTTILNISQNYISELWTSDILSLSK
LRILIISHNRIQYLDISVFKFNQELEYLDLSHNKLVKISCHPTVNLKHLDLSFNAFDALPICKEFGNMSQ
LKFLGLSTTHLEKSSVLPIAHLNISKVLLVLGETYGEKEDPEGLQDFNTESLHIVFPTNKEFHFILDVSV
KTVANLELSNIKCVLEDNKCSYFLSILAKLQTNPKLSNLTLNNIETTWNSFIRILQLVWHTTVWYFSISN
VKLQGQLDFRDFDYSGTSLKALSIHQVVSDVFGFPQSYIYEIFSNMNIKNFTVSGTRMVHMLCPSKISPF
LHLDFSNNLLTDTVFENCGHLTELETLILQMNQLKELSKIAEMTTQMKSLQQLDISQNSVSYDEKKGDCS
WTKSLLSLNMSSNILTDTIFRCLPPRIKVLDLHSNKIKSIPKQVVKLEALQELNVAFNSLTDLPGCGSFS
SLSVLIIDHNSVSHPSADFFQSCQKMRSIKAGDNPFQCTCELGEFVKNIDQVSSEVLEGWPDSYKCDYPE
SYRGTLLKDFHMSELSCNITLLIVTIVATMLVLAVTVTSLCSYLDLPWYLRMVCQWTQTRRRARNIPLEE
LQRNLQFHAFISYSGHDSFWVKNELLPNLEKEGMQICLHERNFVPGKSIVENIITCIEKSYKSIFVLSPN
FVQSEWCHYELYFAHHNLFHEGSNSLILILLEPIPQYSIPSSYHKLKSLMARRTYLEWPKEKSKRGLFWA
NLRAAINIKLTEQAKK

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG)

40. TNF-alpha

Gene Name : TNF-alpha
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 21926
NCBI Protein GI : 7305585
Genbank Accession : AB039224
Protein Accession : NP_038721
Taxonomy ID : 10090
Chromosome No : 17
Gene Starting Position : 35199389
Gene Ending Position : 35201995
Gene Strand (Orientation) : -
Protein Name : tumor necrosis factor
Protein pI : 4.75
Protein Weight : 24010.47
Protein Length : 235
Protein Note : Also known as DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434

DNA Sequence : Show Sequence

>gi|372099093:35199389-35201995 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J
CTTTATTTCTCTCAATGACCCGTAGGGCGATTACAGTCACGGCTCCCGTGGGGAGCAGAGGTTCAGTGAT
GTAGCGACAGCCTGGTCACCAAATCAGCGTTATTAAGACAATTGGGTTAGATAAATATTTTGTTTTAAAC
ATAAGCAAAAGAGGAGGCAACAAGGTAGAGAGGCCAGGTGGGGACAGCTCAGCTCCGTTTTCACAGAAAA
CATGTCTGTCTGAAGACAGCTTCCCACACTGGGTCCTCCAGGACACCCCGGCCTTCCAAATAAATACATT
CATAAGCAAATAAATAAATAATAAATAAATAATAAATAATAAGTGCAAATATAAATAGAGGGGGGCTGGC
TCTGTGAGGAAGGCTGTGCATTGCACCTCAGGGAAGAATCTGGAAAGGTCTGAAGGTAGGAAGGCCTGAG
ATCTTATCCAGCCTCATTCTGAGACAGAGGCAACCTGACCACTCTCCCTTTGCAGAACTCAGGAATGGAC
ATTCGAGGCTCCAGTGAATTCGGAAAGCCCATTTGAGTCCTTGATGGTGGTGCATGAGAGGCCCACAGTC
CAGGTCACTGTCCCAGCATCTTGTGTTTCTGAGTAGTTGTTGAAAGCTCTGAGCACAGAGTTGGACCCTG
AGCCATAATCCCCTTTCTAAGTTAGAAGGATACAGACTGGGGGCTCTGAGGAGTAGACAATAAAGGGGTC
AGAGTAAAGGGGTCAGAGTGGGGGCTGGGTAGAGAATGGATGAACACCCATTCCCTTCACAGAGCAATGA
CTCCAAAGTAGACCTGCCCGGACTCCGCAAAGTCTAAGTACTTGGGCAGATTGACCTCAGCGCTGAGTTG
GTCCCCCTTCTCCAGCTGGAAGACTCCTCCCAGGTATATGGGCTCATACCAGGGTTTGAGCTCAGCCCCC
TCAGGGGTGTCCTTGGGGCAGGGGCTCTTGACGGCAGAGAGGAGGTTGACTTTCTCCTGGTATGAGATAG
CAAATCGGCTGACGGTGTGGGTGAGGAGCACGTAGTCGGGGCAGCCTTGTCCCTTGAAGAGAACCTGGGA
GTAGACAAGGTACAACCCATCGGCTGGCACCACTAGTTGGTTGTCTTTGAGATCCATGCCGTTGGCCAGG
AGGGCGTTGGCGCGCTGGCTCAGCCACTCCAGCTGCTCCTCCACTTGGTGGTTTGCTGAGGGGGGGGGGG
AGGATTGAGTCAGTGTCACCCTCTTAGTTCACACTCCACATCCTGAGCCTCAGCAGCTACCCACACTTCA
CTTCCGGTTCCTGCACCCTCTGTCTTTCCACATCCCATTGGCTATGAGGTCCCGGGTGGCCCCCTGATGC
CTTGCTTTTGAGTCACTGCTCTGACTCTCACGTGCTGTCTCTAAGAGCTCTGTCTTTTCTCAGCCTGGCT
CGACACCCCTCAACCCGCCCCCCAAAATCATGCCCCTTCATTCTCAAGGCACATGTAAAGAAATCTTACC
TACGACGTGGGCTACAGGCTTGTCACTCGAATTTTGAGAAGATGATCCTGGAGGGGAAGAGACAAAGGCA
AGGATGAGCCTTTTAGGCTTCCCAGCAAGCATCTATGCACTTAGACCCCTTTCCTCCCAAACCAAAGCTT
TAAGTTCTCCCCCCACCCCATCTCATCCCATGCCTAACTGCCCTTCCTCCATCTTAAATTAAGAGAGAGG
TGTGGGAACACTTACTGAGTGTGAGGGTCTGGGCCATAGAACTGATGAGAGGGAGGCCATTTGGGAACTT
CTGTGTAGGAAAAGGAGGTTAGTTAAGACAGACTCACCCCAAAGGAGAAGCCTCCCGGCTGATTGCCCCG
CTTACAGTTCCTCTTTGCCCCACCCCACCCCCCAGCTTTGTGTTTTTCTTCTTCATTCATTCATCTGTCC
AACCCACGGCTTCTTTCTGCGGTGCCCTCTGTGCTTGATCTCCCGTTATCTCCCCTTCATCTTCCTCCTT
ATCTCTCATGCCTCTCTCATTTCTGTCTCTGAGTTTTATCTCTTGCTTATCCCCTCTTCCCCTGGCCACA
TCTTTCCAGATCTCTCCACGTGTGAACACACTTGTTCGTTCATTCATCTCTCTGTGCATCCGACGAAGGA
TGTTTAGTCAGCTGGACGCATGGGTCCGAGGTCCTGACTCTGTCCCCTCCACACTCTCCTCCACCTTGCC
CTGCCCATTAGCCCACTTCTTTCCCTCACACTGTCCTTCTTGCCCTCCTAACCCGTTTTGCTTGTGAGCG
AGAATAAGGGTTGCCCAGACACTCACCTCATCCCTTTGGGGACCGATCACCCCGAAGTTCAGTAGACAGA
AGAGCGTGGTGGCCCCTGCCACAAGCAGGAATGAGAAGAGGCTGAGACATAGGCACCGCCTGGAGTTCTG
GAAGCCCCCCATCTTTTGGGGGAGTGCCTCTTCTGCCAGTTCCACGTCGCGGATCATGCTTTCTGTGCTC
ATGGTGTCTTTTCTGGAGGGAGATGTGGCGCCTTGGGCCAGTGAGTGAAAGGGACAGAACCTGCCTGGTT
GGCTGCTTGCTTTTCTGGGAGCTATTTCCAAGATGTTCTGGAGTTTCTGTTCTCCCTCCTGGCTAGTCCC
TTGCTGTCCTCGCTGAG

Protein Sequence : Show Sequence

>gi|7305585|ref|NP_038721.1| tumor necrosis factor [Mus musculus]
MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPL
ISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQ
VLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQL
SAEVNLPKYLDFAESGQVYFGVIAL

Molecule Role : Vaximmutor
Related Vaccine(s): Bacille Calmette-Guérin (BCG) , Mycobacterium tuberculosis fadD26 mutant vaccine , Mycobacterium tuberculosis mce-2 mutant vaccine , Mycobacterium tuberculosis sigE mutant vaccine

41. TSC22D4

Gene Name : TSC22D4
Sequence Strain (Species/Organism) : Homo sapiens
NCBI Gene ID : 81628
NCBI Protein GI : 13569901
Genbank Accession : AC092849
Protein Accession : NP_112197
Taxonomy ID : 9606
Chromosome No : 7
Gene Starting Position : 94697542
Gene Ending Position : 94710214
Gene Strand (Orientation) : -
Protein Name : TSC22 domain family, member 4
Protein pI : 7.24
Protein Weight : 39140.01
Protein Length : 395
Protein Note : Also known as THG1; THG-1; TILZ2

DNA Sequence : Show Sequence

>gi|157734172:94697542-94710214 Homo sapiens chromosome 7, alternate assembly HuRef, whole genome shotgun sequence
ATTCAAGTTTCTTCAGAGTTTTTAATTATTATTATTATTTTAATAACAACCTGAATCCCAGAACTTCCAG
ATACTGTCCACACTCCCTCCCCTGGGCAGAGGAGAGGAGGCACCTCAAGGGAACAAGATGGGGGTGGGGT
GTCTGCCGGGGAGCCTCCGACTCCCCCAAGCCGTCTACTGCTGGGGGGTCCCAGGAGGGAGGGTGGGGGT
TGGTTCCCTCCCAGAGGGGGCTCCCTCTGACGCCCCGTCCTGAAGCCCTCCCTCCTCCATCAAGGCTGGG
GATGATGAGGAGATGGGGCAGGGGCCGGGGGACCAGGCCATTACTGAGCCTTGAACTCCCACCCCGGGGA
CACGGGGACATTAAAGCTGCATAGGAAGAGGGGGCAGGCGGCTGACGCAAGGCCGGGCAGCCCCAAAGGC
ACATTGTAAGGGAAGGGAGGCTCAGACGGAGGGCCCATTGGGCGCAGGGGGCCCAAGCCGTGGGACCCCC
GAGGAGGGCAGCTGAGCCAGCTGCTCCGGGCTGGCCAGGGCGCGCAGCAGCCCATTCTCCTGCTCCAGCG
CAGCGTTCCGCTCCGCCAATTCCCGGATCTGCTCCTTCAGCACCTCCACCTCCTCCCGGACCGCAAACAT
GAGGTGGGACTTCACCAAGTCCTGGGGGCCCCGGGACAGGCACAGCGTCAACGGGGTGGGTGCCTCATCC
CCTGCCCAGTGCCTTGAGGGCTGGGGTGGGAGACAGTCCCCAGTGTCCAGAGGATGAGGGACAAAGCAGG
GAAGGGACAGGGGAAAAGGACGAGGGACAGAGGCAGAGTCAGGGCTCAAAGACAGAGATGGGGACCAGTA
GCCAGACTAGAGGCTGGGCAAAGTCAAGGGTGGAGGTGGTGGCAGGAGGGGGCAGAGGATGCCCAGCAGC
CCAGCAGGGAGAGGGACGGAGGAGCTGGGAGTTGGTGTTCCCTGGTAAGGAAGAGGACAGGGCTGGGGCA
GAGGGATGGCTTAAGGGGGCCAGGACCTCCTGGGACCAAGATGGCTTCTCTTACCATGGCTTGCTCGATT
TTGTTGTCAATGCCAACCAGGCTTCCGGAGCCACTGGAGAGACACAGGAAGGTGAGGGGAGGAGAGGAGA
AGCCTTCCCAGGTGCTGGTTTCTTGGACCACAGCCTCCCGCCCACACCCCCCTGTACCTGTGACAGTAGA
GAATGAGGGAGAGGAGGGTTTTGTCCATCCCCCTGCGGGTTGAAGGCCGCTGCCCAGGGCCTCCCAGGCC
CCGGTGGGGCGGGGGCCCAGATGTCCCTCGGATGGGGGATGCACCCCGGGGAGAGCAAGGGGAAGAGGCA
CAGCCCCTTTTTCGGGGCAGCCCCGGAGCCATCCCCACCACTCGGGTCTCATAAGACGACACCAGGGACT
TGACAGAGACCCCTGGCTGGGCCATGCCCAAGGGGAAAGGGTCCTTAGACACCCCCCCAATGGAGAGGGA
TGCAGTGAAGGGAATGCCTGGGGGGCTCAGAGCCCCCCGGGCAGAGCAGGGTCTGGCTGGTGCCAGGCAG
GCAGAGAGGAGACAGTGCCTCGGGGGTGCTGGCCTGAAAGATATACACCACCCCCCCAAGGGCAAGGCCT
GGCTGCTATTGGCTCCTTGGGGACACGGGGACCCCCCCTCCTTTCAGCTCCTCGGTGGGCGGGAATTCCC
AGACACGGTCAGTGCCAGTGTCCCCTTCTCACCCTGGCACCAATCCCAGAGTGGAGGGGAGGGGGCCGGC
CGAGGAAGTCAGCTGGTGGCTGGGCCAGGGGAGGGGCGTGGGGCAGAAGCCTGGGTCTGAGCGGGGAGTG
AAGGGTGTGAAGGGAGAGGAAGGGGCTGGCCTTCGGAGGCCCCTCGCCACCTCCGACCCCATTCTGTCCC
CTCCGTCACTCAAGGCCTTGGGAAAGAGGGTGGAGGTGGCTGGGGTAGGGGGCGTTCTGACGTGCTCGTG
GTCTTCCATAGATCATGAGCGCTCTCCCACCAAAAAACCCCACAAGGCTCACATCATGGGTGACCCCTGG
GATCTGGCCAGAGGGTCCCTGAGTCTCCAGAGAAGACCAAAAATGGGAAAGGTTACCATGGCAACAGAAA
CTGAGCCCAAAGCCCTAGAAGATGAATACAGCGTAAAGGCCAGGCGTGGTGGCTCACACCTGTAATCCCA
GCACTTTGGGAGGCCAAGGTGGGTGTATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGA
GAAACCCCATCTCTACTGAAAATACAAAAATTAGCCAGGCGTGGTGGAGGGTGCCAGCAATTCCAGCTAC
TCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCCGAGATCGTGCTA
CTGCACTCCAGCCTGGACGACAGAGTGAGATCTTGTCTCAAAAAAAAGCCAGGTGTGGTGGCTCACGCTT
GTAATCCCAGCACTTTGGGAGGCTGAGGCGAGTGGATCACCTGAGGTCAGAAGTTTGAGACCAGCCTGGC
CAACATGGTGAAACCCTGGCTCTACTAAAAGTACAAAAAATTAGCTGGGCATGGTGGGGGGTGCCTGTAA
TCCTAGCTACTCTGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCGA
GATCGTGCCACTGAATTCCAGCCTGGACAACAAGAGCAAAACTCTGTCTTAAAAAAAAAAAAAAAAAAAT
CGCCTATGGGCCTGGGACCTGGGTCTGGGGTCTCAGAGGAAGCGAGGGTTTGAGGAGAGGAAGGGAAGTG
AGCTTGGAGAAGGGACACTGGGAAGAGGAGCAACAGGGCTTGGAAGAGGGCTGGGAGTCTGGGGTGACAG
GGTCTGGCTGCAGTCTGGGAAGTGGCCACCAGAGGGCGACTCAGGCCTGGCTCAACAGCCCCCAGGGCCG
GGCTCCAGGCTGGCTCCACGACGTTGGGCTCCTGGTCCCAGAACCTGGCTCTCCCTCAAGCCCCACTCTC
AGGCCCCACCTGCGCAGGCCCTTGTGCTGACCTCATCCTCCAAGGCTCCCTCTCCCACCCCTCAGGCCCC
AGGCTCTTGCCCTGAGGCTCCCGACCCACCCTTGCACCCCTGCACTCTGGCGCTGTGGCGGGATCCCCAG
TCCTGCCCTCCAGCCCCCAGCTGGGCCCCCCTTGGAGCAGGGGCCTCAAGGCGCTGCAGCCCACATCCCT
GGACTCCCTTGTAAGCCCTCCCTCCTGGCTGTCAGCTCAGACTTGCTTTCCTGCAGGGGGATCAGCACTC
AATTCAAGGTGACCCCTGGATAAGGTCCTGCAGCCAGGAAGGGAGCACAAGGGAGCTGGGAATTTTCCAT
TTCTCATCCCAGCTCTGGGTGCAGAAGGCGCTGACAGGTGGAGCAACTAAGGAGGTGACTAGGCCTGAGC
GAGCAGGATGGGGTGGGTGGGGGAAGGGCATCTGGGACTCTTGGGGTGGTGGGAGAGGAGAAGTTGAAGG
GTGGAGGTGGGTCTGCCAGCAGGAGGGTCAAAGTAATTTTCGAGCTATTTCAATCAGCAACACCTGGGTG
GAGATGGAGCCACCACAGAAAGCCCAATACTGCCACCACCCCATTAAACACTCACAGAGACCAAAGGGGT
TGGATACACAGGGATAGGAACATGGGCCCTGCTGGGCAGAGACTCAGGGGTAAAGATGTCAAGTAAGGTG
TTCATTCTCACCAGAGATAAAGGAATAATACAGCAATAAACGTTTGTTAGCCGACAGGTGACTGTCTTCT
GTTTGTCTAGCGCCTGGGAGTTTTTGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGGGATTTTCCCTCA
TTGGAACCTCCACCTCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCAGAGTAGCTGAGATTACAGGCA
TGAGCCACCATGCCCAGCTAATTTTTGTATTTTTGCCGGGGAGCCTCCGGCAAAATGGGGTTTCACCATG
TTGGCCAGGCTGGTCTTGAACTCCTGACCTCATGATCCACCCACCTCAGCCTCCCATCTCAGTCCTGGGA
TTACAGGTGTGAGTCACTGTGCCAGGCCTCTATTGGAACATTCTAACACACACTTCAAGGGACCAGGGCT
CTGGCAATGCAGGCCCTTCCCTTTAGGACCCCAAAGCGAGGGGTTGTGGGCACTGTGCTAGAATTCTTTG
ACAGGGTCCGGGGGAGGAAAGTAGGGGCTGCTGAGACAGGATCGACCTCCACCACAAGCCCAAAAGCAAG
TTTGTATGGATTAGAAAATGCCCCCTTCTTCGAGATCCTTAATGCCATCTTCTGGAAAACTGTCGTGACT
TCAAGCATTGACTGTGATTTCAAATATGAGCGGCACCCCCTGGAGTTAGTTGTGCAGTGTGTGACATGCA
CAGTGGGAGACTTAGCCCTGCAGGACTCTTGGTCCTGGGGCGGGGACAGCCCTAGAAGCTGGTCTTCCTC
TTTGAGGGTCAAATAAAACGGCCTCAACATGAAGATGAGATAAAAAGCAGGACACCTGGGAGAAATGGGG
AGCCTGGGATCTTGGGGACAGGGGCCAGCCCTGCACACTCTCCAGTGGGTTCAGGGGATCCCCCAGGGCC
TAGGTCTGGGGCCAAGAAAGTGTCATCCAGCTGCCCTGTCTCTATGCTGTCCCGGATCTGTGGTGTCTTT
GGGGCGGGGCTGGGGAGGTCGGGGAGGGGGAAGGGGGGCTGGCTCCCTGCCCTGCTTGGGAGAGCTGTAC
CTGACTGTCCTAGAGAGAAAGGATATTGTAGACCATTGCCGCCACCTCTTTGCCTGGGACACAGGCATCC
CAGTAGGACAGTGGAGAAGGTGTGGCTTTGGAGTTGGACCCAGGCTCCAGCTGCAGTCCAGCCACTTAAA
CTAGCTGGGTGGCCCTTGGGCAAGTTTCTAAAACCCTCCTGGCCGGGTGAGGTGGCTCATGCTTGTAATC
CCAGCACTTTGGGAGGTCGAGGTGGGTGGATCACCTGAGGTCAGGAGTTTGAGATCAGCCTGGCCAACAT
GGTGAAACCCCGTTTCTACTAAAAATACAAAAATTAGCCGGGCATGGTGGCATGCACCTGTAGTCCCAGC
TACTGGGGAGGCTGAGCCAGGAGAATCGCTTAAACCTGGGAGGCAGAGGTTGCAGTGAGCCAAGATCGTG
CCACTGCATTCCAGCTTGGGAAACAGAGCAAGACTCCATCTAAAAAAGAAAAAAACAAAAACAAAACAAA
AAAAAACCTCCTTCTCTGAGTCTCAGATACTTCATCTGGCAAGTGGGAGAATGGAACTGCTTTTTAGGAT
TAGAAGGGAGAAGGCAGTACCGGACCCAAGGGAGGCACTGGATAAATAAATGTACCTTTTCTTTCCCAAG
TCCCTGGGATCCATTCTGTATAATGCCTGGATCCCCCAGGCCGGTCACTGCCCCCACCCCCATTCCTTCC
ATCCACTTCTTCACACCTTAATTACCTCCTGCCTGCCGGGAGGGGACAAGGATTTCCAGCCCTGGGGCAC
GTGAGCATTCTAGATTCACCTGCACCCTCTGTTCGCCCATCCCCAGATTCAGGGACTGGTCAGGCGTCCC
CAACTCCCTCCCTGTGATCCAGGCACTGAACATCTCAGCCCACCTTCCCTCTTAAGAGAGAGGTCCCAGG
CCACTGGCATCCTGTCCTGCCAGCCCTGTGCACACCAGACAGGAGAGGTGGGGCCTACATGTCCCATTCC
TAGGGGGCAGGGGGTGGCCCAACCCAAACCCGCTGGCCTGGCAGGGTGGGGCTTGAGGGGCTGGGCTGGG
ACAGATCCCCAAGAGCCTTGGAGCACAGCAGGGTGAAGTGGAGGCTGGGAACCGGACCGGTCAGATTGGG
GTGTGCAGGGAGGGGGTGGGATGGAGGGAGGCAGGGAGGGAGGGTACAGGGGAAAGACGGAGGCCTCAGG
AGGTTGAATCCTCTAAGACAGAGACAGCAGGAGGCTGGGCTGTGAACTCAACAGGGGTGGGGGAGGGACG
GGGAGTCCTCTTGCCGGGGAACCTCCCAAGTGGGAGAGGGAGAGGGCGGGAAACGCTGAGAATAGCAGTG
ATGGCGGCAGGTGACTCTCCCAGCCCTGGCCGACCTCACACCTCGCACATTCCTGAGAGTGGGAATGGGG
CGTGCAGAAGCCACCCAGTCCTGCCCAGCCCCGCCCAGCCCCACCCAGCACTGGCACTCTTGCCCTCCGT
CCCACCAGCTCCTGGCCACCTCCTTTGCCAGGTCCCTATCCTCCCTGAGGCCGCAGGACCCTATCAGTGG
TGGGCTTGAGTCCCTGATCCCAGCGAGTGGCCCCTGATCTGGGGAGGACAGGAAGGAGCGACTCCCATCT
GGAATTGGTCCCTCAGGCACCCACTCACCCACTCTCGAAGCCCCACCCCCAATTTCCTTCCAAGCAGGAA
GTCCCTCCCCTTGAAAACCTCCCACGGGAAGTCCCACTTCTCCCTCCAAAAGAAAAGCGGTGACTCCCCA
GCCCTTCTGCCCGGTTCCTATCAGTCCCCAGGGAGAAGCAGGCAGCCTGGCTATCTCTGACTCACGATTC
TGGGCCTCCCTTTTCAGCTTGCTGGCTTTCTTAGGAATGGTGACACCTGGCCCTGAAAACCCACCCAACA
GAGCACTGTCCCCTAAAGGGAGAACCCCAGAAAGTCGCTCTCTGCAGTGCTGCCCCTGCCCCTTTGCCGT
TCCTAGCTCTGATTCTGTTCCTAGCTGGCCCATCTTAGGGACTTGCTTTCTCGGTTCTAGAAATCTTAGG
ATCCTAAAGTAGACCCTGTGGGCTATCAGGATGTCACGCTCCACAGGTGCTGTTAGGGAGTCCCAGTCCT
TTGTTAGGAGAGAAAGAGGGAGATGGAACTGGGAGGAAACTGATTTCCATTCTTTTTCAGTTTTTTTTGT
TGTTTTTTTTTTGAGGCGGAGTCTCGCTCTGTCACCCACGCTGGAGTGCAGTGGTGCGATCTCGGCTCAC
TGCACCCTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCA
CGTACCACCACGCCTGGCTAATTTTTGTATTTTTAGTACAGACAGGGTTTCACCGTGTTGGCAAGGCTGG
CCTCGAACTCCTGACCTCAGGTGATCTGCCCGCTTCAGCCTCCTAAAGTGCTGGGATTACAGGCATGAGC
CACCATGCCCAGCCTACTTCCTTTATGTATATATATATATATTTTTTTTTTTCTTTCTTTCTTTTTCTTT
TTAGAGATGAGGTCTCGCTACATTGCCAGGGCTTGGTCTTGAACTCCTGGCCTCAAGCAATTTTCCTGCC
TGGGCCTCCCAAAGCACTATGAGTACAGGCGGGAGCCACTGTGCCCCGGCCTGATTTGCTATTCTGTCTA
GAGCATGCATTTGCCAGTGGGTTCCTGGGTTGGCCAGATGATCTCTAAATCTGCAAGAGCTCTGGAATCT
GACAACAATGACTCCCTTCAGACTCTAAAAAAGTCTGTGACCTGACAGTTCTGAGCCAGGGAGTCCCACC
CAGCCCTCTCCACAGAGAGGGAGTGGGTCCGAAATCAACTGTGTGCAGTGGCTATGCCCAGGCCAGGTTT
CTCTAAGAGGTCCTCTCCAAGTTCAACCTGGGGGAAGGATGCCTACCAGGCACTTTCTTACAGCTACCCC
GGGTGCTGGGCGGGGAACCTGAACCCCACGCCCCACCACCCCACGAAGCCCACCTACCTATCATCGTCGC
TGTCTAGGTGACCACTGATGGCCAACATGGAGTGGGCCAGGCTGAACTTCTGAGCTGCTACTGCTCCGAA
GGGGTCTGGAGATTTGTGAACCAGGCTGGCATCGTGGGTGAAGTAGAGGGCTGGGGAGCTGGGGCGAGAG
TCAAGTGGGGGCACCTGGAGGCGGAGAGGTAGGAACCATCACATGAAAAGAGAAAAGAAAGGAACCAGCT
GCCTTAAAACTTGCCTATTAGCCTTCCTCCCTCTCCACCTCCCCACTCTCTTTCGAGGACCCAGGAGTCC
TGTCCCCGCAGTCCTTCCCTCCTCCAGCTCTGGAGAAGGCATTAGGCAGGTACTAGAAGCAAGAAGTGGG
TCAACCTAAGGAATGGGAGGAGTGGTGTATAGGACATTAGAGAGAGTCTCCCAAGTGGGAGGAGGGGGTG
ACTGACGGAGGAACCCAGAATAGACTCCAATGCCCCAGCTCCCACTGGGCCGGGACCCCTAATCAGAGGC
TACGCAGCGTGTCTGCCTTCTTTTATGTTTTATTTTTATTTTTTTGAGACAGTCTCACTCTGTCACCCAG
GCTGGAGTGAAGTGGCACAATCATGGCTCACTGCAGCCTTGAATTCCTGAGCTTAAGCGATCCTCCAGCC
TCAGCCACCCGAGTAGCTGGGACTACAGGCATGCACCATCATAACTGGCTAATTTTTTATTTTTGGTTAA
CACAGGGTCTCAAGATGTTGTCCCGGCTGGTCTCAAACTCCTGGGCTCAAGTGATCCTCCTGCCTCAGCC
TCCCAAAGTGCTGGAAGAATGAGCCACCACTCCCAGCCTGTGCCTTTACTGGAGTCTCGCTCTGTCGCCC
ATGCTGGAGTGCAGTGACGTGAACTTGGCTCACTGTAACCTATGCCTTCCAGGTTAAACTGATTCTCCTG
CCTCAGCCTCCTGAGTAGCCGGGACTACCGGCACCTGCCATCACACCTGGCTAATTTTTTTATTTTTAGT
AGAGACGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGATCTGCCCGCC
TCGGCCTCCCAAAATGCTGGGATTACAGGCGTGAACCACTGCACCCAGGCTGCCTTCTTAAAATGACTCC
AATGTCACCTCTTAGAGAGGCATTTCCCCATCATTCACTGGTTTACTGAACTATTTCATTTTTTTATAGC
ACCTAACATTATCTGAAATTAAAGTATTTATTGTGTCTCTCCCCTGCTAGAAGGTCAGTTTGCTCAGTAG
CTATTTGTAGAATGAATGGATCCTCAGAAACTGCTCCTCCATCCCCTCACCCACCCCCCACTCTCGCTTC
CCCTCTCAAACTTCTCGGACTCAGGTGGAATTCTGCTCCTGCGCTCTGCCCTAGGGTTGGGGGGGGAGCT
GGTTGTGGAGCTGGGGGGAGGCGGAGCCTGGGCGTGGACCTGCATAGCCCAACAGGCTCTGGGGGCAGCA
GCAAAAAGGGCGGGGGCGACAGGAACATGACAAGACGCCTCTGTGAGGCCCCGGGAGGCCCCAGCTTCTC
AGAAAAGCCAAGAACAGAGAGTCCAGGGTGGTTAAGGGACAGAGCAGAGGAAGGGGGCCCAGGTGGTGTC
CCCTGGCCCTTCTCCACCACTCCCCTCTGTTAAGAGGGAGAAGAGGCATGAGGGGACGTGGAAGGGAGAC
AGACTTTTGGGCAAGGAAATAGCAAAACAGATGCGTGCAAACAGAAGACCGTCAGGAGGTGGGGGAAGGC
TGGGGAGTAGGGGGCACTGAGAGAGAGAGGCAGGGTCTGTGGCCCCAAGAGAAGCTCAGGCCAGGCTTAA
GGACAGAGAGCAGGGAAGCTGGAGGCCAAGGGGGTTTGCTTTGGAAGAGTCTGGGGACAGGACAGGAAGA
GAAACAGCATCTTCTCCCCTGGGGTGGGTGCTGAATGCAAGAGTTCAAGAAGAGTTGGGGGAGGGGGGGT
AGGAGGGGGCATCACGAGAGATGTGAGCTTGAGGCCAGGAGGGGTTTGGGGGGAGCCAGTCCTGACCGGT
CCTGACAGGACCCAGACATCCAGTCCCAGGTTATTTTTATACTGTGGTTTGTGCCCAAGGCCAGGAAGGG
GAAAGAGTTCGCATCCTCACACTCCCTGCCTTTCCCCAGACCTCCGAGGTGTTATATTCTCCCACAGGAC
AGAAGTATCCAACCTTCTATCTCTGGCTGCTCACCCCCACCATGGGGACCCAGGCATCCTGCCCCCACAG
CCTGGCTTGTTCCTCTTTTGGAGACAGGAAGGAGAAAGAGTATGGGACTGAACTCCCTTCTGGGGCTCTT
CTCTCCCACTCCACAGTGGTCACCCTCGAGTTATCAATCACAGAGAGGCTCAGATGGACGGGGGTGAGCC
AGACAACTGGGGGGTGAAATGGATCAACCAGCAGGCCCCCTCCCTGTCCCTGAGAGGTGTTCCCCCCACC
CAATTTCTCAGTGACACTTGGCTTACAGCAAAGCTGGAGGCAGGACTGAGATGGGTGGCTGGGGGAAAAG
AAAGACCCCCACATTCAGGATGGGGCTGCAGTGCAGAGACTGGCAGCGCGCAAGGCCTACCTTTTACTTT
TCCTCCCCAAATCCTGGCAGCTCCTCCCCTAGGGACCCAGCTACCCAGCTATCCAGCTGGGGCCTCCACC
CTCATCACCTAGAGGCAGAGGGGCTGAGACCAAGACAGAAGAAGGCCTGATGAGGGGGCCACATATGCTA
CAGGCTACATGAAGACCAAGACAGAGAATCAGAGGCCAGGGAGAAAGAGAGAGGGTGCAGAAAAACAGGA
AGGAGGCTGAGACAGAAAATGAAGTAGGAGGAAGGGGCTTCAGAGTGACCTGGCAGGCACAGAGAAGAGG
GCTATCTGATCTTATAAAGTGATGGAGAAGGAGGAGGAGAGGGGGGGGAGGAGGAGGAAGGAGGCTCAAG
ATAGAGGTTAGGCCAGGGCTGATGGGCCAGCCCTAGAACCCAGGTCTTACCTGCCCCATCTCTTCTGGAG
CACCCAACTCCATCCGCAGCCGCATGTCTACAGCTTTCCTCCGGGACAGTGGAGGGGTCCTGGCCCCTGA
GCCCCCAGCCTCCGCTTCCACCCTCAGAGAGGGCAGGGGCGTGGCAGCCCGGGATGTGCCCGCACTCTCA
CCGGTCTCAGGCTCTGGGGGGCGCTGCTGGGGTGAGGAGGCCGACAGTGGGGGTTTCTCTGCCTTGGCTT
TGCTGGGCACCACCAGCTGGCCCAGTCCCCCAGTGAAGGAGCGGGCCTGAGGTCCAGGAGAGGTGGGGGG
TGGACGGAGCCAGGAGGTGGGGCCCTGAGACAGGCCTGACGGTGGGGTGGGGGCGCCCAGGCCGAGGCTG
GCCAGCTCCAACCTGGAATCCAAAGATCTGCCCCCGGCGCCCCCTGAGGCCCCTCGAATTCCCTCCAGGA
GTCCGCCGAAGCTGTGGGGCTCCAGGTCTCGCTCATAAACATCCACACACGTCCAGCGACCGCGGCGATA
AGGCTCTCCCAGGCCGTGGGGCAGCTTCACCACCCGGAAACGGGAGGAAGGGGCCCCAGGTGGTGGGGAG
CCATTCCGGGGGGTGCCCTTGCCCCCCGGATCGGGGCTGGGCTCCCCATTGGGCAGGCGGGGCGGGGGCC
CGGTTGGGGGCTGTGGGGTAGGGGGATCCGAAGCCCCTGGGCTCCCAGGGCCCTCATAGTCCGTGGTGAC
GCTGGTGATTTGGAAACTACTCTTCTTCTTGCCCCCGCTCATGGTCCCTGGGGCTCAGGGCTGGGCCAAG
GTTGGGGGTGGGTTGGGGCTCCTTGAAGGGGCTCAGGCACCCCTGGAACAAGGGGGCCACATGGCGGGGA
CATCCGAGCCCCTGGGGACGTCTGGGTCCGACATGGCAGGAGGGCCTGGCGGGAACCGGGGGTGCCTGCT
GGGTGAGGGGAGAAGAGGAGAGGGGAGGTGGCGGGAGGGGGGAGCAGGGGCAGGTTTTTCCTGCTGCTGT
TGCTGCTGAACTCCAGAGCTGAGTTTGCAAACTGGAAAAAGAGGGGGAGAGAGAGAGAGAGAGAGAGAGA
GAGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAAACCAGTGGGCAGG
CCACCTCCGGGGCTTCCTCTCCGGGGAGCAGAGTGGGGGAAGGGGAAACCGTCAGAGACCCAGAGACAGG
CAAGAGATGACAGAGACCAGGGCAGAGGGGACTGGATCCAAGAAGGCACTCAGAGTAGAAAGAGGCAGAT
GAGTCCCAGAGTTGGGCATTTCTCGCCTGGGATTCTGACTCAGGGGGCCCGTATTGTCCCAAGAGCTCGG
ACTCCAGGGCCTGGGAGAACCAGGCTGAAGCAGCTCCCCAGCCTGGCCCGGCCACTCAGAGAGACCTCTC
CTGGGCCTCGAGCCCCCACCCTCACCCCAGCTCCCACATCTCTGGGGCAGGCCCTGCCCGGCTACCCCCC
AGCGCCCCACCCAGCCCTGAACCCTACCTCTGGGGCCTCGGCCACTGATCTGAGCCTGAGGGTCCCGGCG
CCTAAGAGGTCGGGCTGTCCGTTCCCTGGGGCCTCCTGGCCATGGGCAGTGGCGGGGCACGCAGGCGGCG
ATCAGAGGCTGTCCCGTCCTCTCCGGGGGCCGCGGCTCATCCTGCCAGGCATCTCCGAGGAAAGTTTGCT
CTCCGGAAAAGAAGAAACCCGCGCCTCCTCCGAGGAGCCTAGGAGCGGCCTCTGAAGCCCCAAGGGTCTG
GAGACTGATGTGGGAGGGGGCGGCCGAGCCCCCCTCTGCTCCCGGTGGCAGCCAGCGAGTGGGTGTCCCG
AGTCTCCTCCCGCGTGACCCTGCTGGGTCCGTGTCTCCGCTCCTCGGCTCCCCTGGGTCTCTGCCTCTCC
GTC

Protein Sequence : Show Sequence

>gi|13569901|ref|NP_112197.1| TSC22 domain family protein 4 [Homo sapiens]
MSGGKKKSSFQITSVTTDYEGPGSPGASDPPTPQPPTGPPPRLPNGEPSPDPGGKGTPRNGSPPPGAPSS
RFRVVKLPHGLGEPYRRGRWTCVDVYERDLEPHSFGGLLEGIRGASGGAGGRSLDSRLELASLGLGAPTP
PSGLSQGPTSWLRPPPTSPGPQARSFTGGLGQLVVPSKAKAEKPPLSASSPQQRPPEPETGESAGTSRAA
TPLPSLRVEAEAGGSGARTPPLSRRKAVDMRLRMELGAPEEMGQVPPLDSRPSSPALYFTHDASLVHKSP
DPFGAVAAQKFSLAHSMLAISGHLDSDDDSGSGSLVGIDNKIEQAMDLVKSHLMFAVREEVEVLKEQIRE
LAERNAALEQENGLLRALASPEQLAQLPSSGVPRLGPPAPNGPSV

Molecule Role : Vaximmutor
Related Vaccine(s): Mycobacterium tuberculosis sigE mutant vaccine

IV. Vaccine Information

1. Ag85B and ESAT-6 fusion protein

a. Vaccine Ontology ID:

VO_0000536

b. Type:

Conjugate vaccine

c. Antigen

Two immunodominant antigens, Ag85B and ESAT-6, were used in the analysis. ESAT-6 is a 6-kDA early secretory antigenic target. The two antigens are combined as a fusion protein in the vaccine (Olsen et al., 2001).

d. Gene Engineering of EsxA (ESAT-6)

Type: Fusion protein ESAT6-Ag85B
Description: Fusion protein created in E. coli using His-tag cloning system pMCT6. Proteins were affinity purified, separated via ion exchange chromatography, and analyzed with SDS-PAGE and Western blots (Olsen et al., 2001).
Detailed Gene Information: Click here.

e. Gene Engineering of Ag85B from M. tuberculosis H37Rv

Type: Fusion protein Ag85B-ESAT6
Description:
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0001250
Description: 1 25 μg amount of monoposphoryl lipid A (MPL) adjuvant was added to 250μg dimethyl dioctadecylammonium bromide (DDA) emulsifier per 0.01-50μg of vaccine.

g. Adjuvant:

VO ID: VO_0001261

h. Preparation

M tuberculosis Erdamn and H37Rv were grown in media, while BCG Danish 1331 was acquired as a freeze dried-vaccine and replenished (Olsen et al., 2001). Recombinant ESAT-6 was obtained along with short-term culture filtrate. Esat6 and ag85B coding regions were obtained by PCR amplification from M. tuberculosis H37Rv chromosomal DNA using selected primers. PCR products were digested by two restriction endonucleases and cloned into pMCT6 prior to sequencing. The E. coli XL-1 Blue was used to express the His-tagged protein prior to purification, protein anion-exchange chromatography, and dialysis. PCR fragments per gene were joined at a HindIII site, cloned into pMCT6, expressed, and later purified to create a fusion protein and a chimeric plasmid.

i. Mouse Response

Host Strain: C57BL/6J (H-2^b), specific pathogen-free female; B6CBAF1
Vaccination Protocol: Female mice 6-12 weeks old were housed within a BSL-3 enclosure. Mice were immunized with 0.01-50 μg vaccine emulsed in 250 μg dimethyl dioctadecylammonium bromide (DDA) and 25 μg of monophosphoryl lipid A (MPL) aduvant. Vaccines were injected 3 times subcutaneously on back at 2-week intervals (Olsen et al., 2001). BCG Danish 1331 dose (5x10^4 baccilli/mouse) was administered via subcutaneous injection at base of tail simultaneously with first subunit vaccination. No booster injections were included (Olsen et al., 2001).
Persistence: Mice were sacrificed at either 2 or 6 weeks post-innoculation via i.v. or aerosol routes, respectively prior to organ removal and bacterial enumeration(Olsen et al., 2001).
Challenge Protocol: Prechallenge immunity was assessed 5 weeks post-first vaccination (Olsen et al., 2001).
Mice were challenged at either 10 or 30 weeks post-immunization via aerosol route (100 CFU of M. tuberculosis Erdman per lung) or intravenous route (0.2 ml volume containing 5x10^4 CFU M. tuberculosis H37Rv in PBS) (Olsen et al., 2001).
Efficacy: A statistical reduction in the number of bacteria was observed for vaccine doses as low as .01μg. The 0.1-10μg dose range showed a higher level of protection versus the .01μg dose. 50 μg doses resulted in reduced protection in lung and spleen, whereas, 10 μg showed significant levels of protection in spleen. The fusion protein Ag85B-ESAT-6 showed a statistically significant improvement in reduction of bacteria versus the individual Ag85B and ESAT-6 components (Olsen et al., 2001). Both recombinant proteins induced long-lived memory immunity when challenged 10 or 30 weeks post-first vaccination, while BCG showed significantly lower protection levels at the later challenge points(Olsen et al., 2001).
Description: Blood lymphocytes were purified and pooled from eight mice per group and cultured in triplicate as 2x10^5 cells in 200μl of RPMI 1640 with 2-mercaptoethanol, glutamine, penicillin-streptomycin, and 5% v/v fetal calf serum. Mycobacterial antigens administered as 5-1.3μg/ml. Interferon gamma amounts were determined using ELISA (Olsen et al., 2001).

2. Arabinomannan-tetanus toxoid conjugate

a. Vaccine Ontology ID:

VO_0000528

b. Type:

Conjugate vaccine

c. Antigen

Arabinomannan (AM) oligosaccharides. They are derived from Lipoarabinomannan (LAM), a carbohydrate antigen expressed on the surface of mycobacteria which contains a manna polysaccharide core attached to a phosphatidyl inositol lipid moiety (Hamasur et al., 2003). The surface carbohydrate was hypothesized to yield CD1-specific immune responses, promote mycobacterial clearance, downregulate T cell proliferation, and interfere with activation of macrophages through interferon gamma intervention (Hamasur et al., 2003).

d. Gene Engineering of Ag85B from M. tuberculosis H37Rv

Type: Conjugate protein purified from LAM
Description: The 28-kDa AM oligos were pooled and linked to tetanus toxoid (TT) or other M. tuberculosis proteins (Ag85B, 75-kDa protein) with thioether linkage generated by conjugation method. Conjugation consisted of amination of AMO with ammonium chloride and sodium cyanoborohydride, deasalting and treatment with 2-imminothiolane, and end conjugation between thiolated oligsaccharide derivatives with bromoacetylated proteins and tributyl phosphine (Hamasur et al., 2003).
Detailed Gene Information: Click here.

e. Adjuvant:

VO ID: VO_0000127
Description: Alum (aluminum hydroxide gel) adjuvant was used initially. Eurocine L3 suspension adjuvant was prepared using 1:1 ratio solid monooleate and oleic acid, Tris buffer with pH adjustment including NaOH, and subsequent sonification. Eurocine L3 emulsion was prepared using 1:1 ratio solid monooleate and oleic acid which were liquified prior to addition of soy bean oil (Hamasur et al., 2003).

f. Preparation

Arabinomannan (AM) oligosaccharides derived from LAM of Mycobacterium tuberculosis H37Rv were isolated and covalently conjugated to tetanus toxoid (TT) or to short-term culture filtrate proteins (antigen 85B (Ag85B) or a 75kDa protein) from M. tuberculosis strain Harlingen (Hamasur et al., 2003).

g. Mouse Response

Host Strain: C57BL/6, 8-10 wk female
Vaccination Protocol: Mice were injected subcutaneously in flanks with 100μL or intranasal with 5μL/nostril of antigen plus adjuvant. The control BCG vaccination involved subcutaneous injection of 5x10^5 CFU in 100μL PBS in hind flank. Two formulations of the adjuvant were used, such that mice were either treated at day 0 with AMOs-TT conjugate in Eurocine L3 emulsion or suspension, followed by nasal booster at day 21 (Hamasur et al., 2003).
Immune Response: Immunization with AMO-TT, AMOs-Ag85B, or AMOs-75kDa conjugate resulted in increased lymphocyte proliferation in spleen when responding to PPD (Hamasur et al., 2003).
Challenge Protocol: Mice were challenged intranasally with 10^5 M. tuberculosis Harlingen on day 24 (vaccination on day 0).
Efficacy: All mice started to die after 17 weeks (median time = 36 weeks) (Hamasur et al., 2003). Considerable increases in weight were observed for mice immunized with AMOs-TT conjugate or BCG versus those not vaccinated (Hamasur et al., 2003). Mice immunized with AMOs-TT conjugate and either adjuvant showed longer survival than sham-immunized mice. The emulsion adjuvant showed increased protective efficacy versus the suspension version (Hamasur et al., 2003)

h. Mouse Response

Host Strain: C57BL/6, 8-10 wk female
Vaccination Protocol: Mice were immunized subcutaneously on days 0, 28 with 5 or 25 μg of AMO-Ag85B in 1% alum. Control mice were vaccinated s.c. with live BCG on day 0.
Side Effects: Reduced weight loss observed for BCG and AMO-Ag85B immunized mice versus shams (Hamasur et al., 2003).
Challenge Protocol: Mice were challenged intravenously with 10^5 CFU M. tuberculosis H37Rv on day 60(Hamasur et al., 2003).
Efficacy: BCG-immunized mice exhibited best survival versus mice immunized with the AMO-Ag85B vaccine. However, AMO-Ag85B immunized mice also showed significant improvement in survival versus sham-immunized mice.

i. Guinea pig Response

Host Strain: Dunkin Hartley, female (250-300g)
Vaccination Protocol: Guinea pigs were immunized s.c. (day 0) and boosted nasally (day 24) with Eurocine L3 emulsion, AMO-TT conjugate, and variable antigen doses and adjuvant concentrations (Hamasur et al., 2003). A protection study included immunization with 15 μg AMO-Ag85B in 10% Eurocine L3 emulsion. Comparison BCG groups were injected s.c. with 5x10^4 CFU live BCG (Hamasur et al., 2003).
Side Effects: Five of six animals non-vaccinated and two of six AMO-Ag85B vaccinated were killed before 120 days post-challenge if observed >20% loss of starting weight.
Challenge Protocol: Guinea pigs were aerosol challenged with saline suspension containing 10^6 organisms per ml (~10 CFU/lung).
Efficacy: BCG vaccinated animals showed reduced counts of M. tuberculosis bacilli in lung and spleen samples versus saline controls. The reduced CFUs in spleen samples along with histological sections analyzed with double blind analysis suggested an overall decrease in disease severity in lung and spleen (Hamasur et al., 2003).

j. Rabbit Response

Host Strain: White New Zealand female rabbits (2.5-3kg)
Vaccination Protocol: Rabbits were immunized with LAM (50 μg), AMO-TT (20 μg), AMO-Ag85B (20 μg), or AMO-75kDA (20 μg) conjugates via i.m. injection with 100 μl PBS and 100 μl FIA emulsifier, and later boosted (weeks 2, 4) with same dose of the previous vaccine.
Immune Response: LAM alone did not induce LAM IgG antibodies, whereas the three conjugate vaccines induced high IgG antibody titers (Hamasur et al., 2003).
Challenge Protocol: No challenge protocol implemented for rabbit immunogenicity study.
Description: Purpose of the experiment with rabbits was to determine immunogenicity response to LAM and AMO-protein conjugates.

3. attenuated M. bovis strain WAg539

a. Vaccine Ontology ID:

VO_0000543

b. Type:

Live, attenuated vaccine

c. Antigen

M. bovis with gene mutations due to signature-tagged mutigenesis to create attenuated mutants. Ten genes were found to be deleted or interrupted in the 10.1-kb deletion in WAg539, including mmpL4, mmpS4, ufaA1, and sigK, as well as unknown genes (Collins et al., 2005).

d. Preparation

M. bovis strain and its recombinants were cultured in Middlebrook 7H9 (Difco) liquid medium supplemented with albumin-dextrose complex (Difco), 0.05% Tween 80, and and 0.4% sodium pyruvate and on Middlebrook 7H11 (Difco) solid medium supplemented with albumin-dextrose complex and 0.4% sodium pyruvate. Recombinants were cultured on kanamycin-supplemented medium. M. bovis culture from pig spleens were homogenized, filtered, and further processed (Collins et al., 2005). Forty-five recombinants with unique tags were subcultured separately and pooled together. The forty-five M. bovis recombinants were subcultured separately, and 10^6 CFU of each pool was inoculated subcutaneously into the flank of each guinea pig. Splenic culture were extracted from sacrificed animals and used to isolate 500-2,000 colonies per spleen. Tags identified in both the inoculated and recovered pools were amplified by PCR and processed.

e. Description

Signature tag morphogenesis was used to generate illegitimate recombinants of M. bovis. Several of the recombinant strains produced protection that was "at least as good as that provided by M. bovis BCG" in guinea pig models (Collins et al., 2005).

f. Guinea pig Response

Host Strain: Dunkin-Hartley
Vaccination Protocol: Groups of six guinea pigs were vaccinated with 10^5 CFU of one of the avirulent recombinant strains or BCG. Six animals were not vaccinated (control group) (Collins et al., 2005).
Challenge Protocol: Guinea pigs were challenged by aerosol with 2 to 10 CFU of wild-type M. bovis eight weeks post-vaccination (Collins et al., 2005).
Efficacy: Four mutants which did not induce any visible lesion were tested for vaccine efficacy. In the case of three mutants, animals were sacrificed 5 weeks post-challenge. The final mutant, WAg585 were sacrificed at 8 weeks post-challenge. Mutant WAg539 gave better, though not significantly different, protection than BCG vaccine, as well as the best vaccination of the four mutants (Collins et al., 2005).
Description: Animals were sacrificed and autopsied 5 weeks after challenge to enable the following analyses: weight, gross pathology, mycobacterial culturing and enumeration in splenic and lung tissues, and statistical analysis of macroscopic lesion count and other quantifiable measurements (Collins et al., 2005).

4. Bacille Calmette-Guérin (BCG)

a. Tradename:

BCG

b. Vaccine Ontology ID:

VO_0000771

c. CDC CVX code:

d. Type:

Attenuated live vaccine

e. Preparation

The evaluation of BCG lacks comparability among the vaccination studies due to the use of six different BCG sub-strains [Copenhagen-1331, Pasteur-1173P, Glaxo-1077, Tokyo-172, Russian, Moreau]. The comparative genomic analysis of different BCG strains has shown that there is no uniformity among all presently used vaccines and this genetic variability may contribute to the variation in protective efficacy seen in different geographic regions of the world (Gupta et al., 2007).

In one case, Calmette et al. (1923) used decreasing doses (1 mg, .01 mg, and .002 mg) of tubercle bacilli to infect guinea pigs s.c. Bacterial loads increased in response to decreasing dose sizes. Similar results were acquired for later intramuscular and intracerebral infection procedures by other groups (SWEDBERG, 1951)

f. Virulence

The original BCG strain lost its virulence after 39 passages by Albert Calmette and Camille Guerin, and was administered to humans i.d. on the 231st passage. The BCG bacteria showed altered colony morphologies and no virulence in experimental animals. BCG has shown a high degree of safety over the years (Ducati et al., 2006).

--Calmette et al. used length of survival as a metric for virulence in their 1923 study (Agger et al., 2002).
--The increased ability for M. bovis strains to retain virulence was noted by Lange in 1922 (SWEDBERG, 1951).

g. Description

Albert Calmette and Camille Guerin, two French scientists, made an attenuated strain from another mycobacterium (M. bovis) by growing it for 13 years on culture. They observed a reduction in the virulence in animals through this period. When infants were given this vaccine, it provided a reduction in mortality by 90%. Since then, Bacille Calmette Guerin [BCG] has become the most widely used vaccine throughout the world. It is estimated that more than 3 billion people have received BCG. The protective efficacy of BCG vaccine against adult pulmonary TB has varied dramatically from 80 to 0% (Gupta et al., 2007).

Development of the BCG strain was predated by numerous M. tuberculosis studies from the 1890s-1920s, including (Lote 1889, Strauss 1895, Moriya 1909, Romer 1903, Weber 1912, Binder 1915, and Browning and Gulbransen 1926) in a diverse set of hosts (including mouse, avian, human, bovine, and guinea pig hosts) (SWEDBERG, 1951).

Several new subunit booster vaccinations are under development, including Ag85, Mtb72F, HspX and other DosR-controlled gene products, Rv3407 and other Rpf-controlled gene products, and heparin-binding hemagglutinin (HBHA) (Kaufmann, 2005).

h. Human Response

Immune Response: TB Ag-specific mucosal, humoral and cellular immune response via T cell proliferation and production (Wang et al., 2009).
Description: SL(E6-85B) vaccine, when combined with BCG vaccination, induced the strongest TB Ag-specific mucosal, humoral, and cellular immune responses comprised of increased proliferation of T cells, IFN-gamma expression, granzyme B production (Wang et al., 2009).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced significant TB Ag-specific IFN-gamma immune responses in splenocytes. Splenocytes were stimulated with the Ag85B protein 2 weeks after the final immunization (Wang et al., 2009).
- Detailed Gene Information: Click here.

i. Human Response

Vaccination Protocol: DNA was obtained from the peripheral blood of 99 NMIBC patients who were prospectively randomized to receive postresection intravesical BCG or BCG with interferon alpha. (Chiong et al., 2011)
Immune Response: Patients with the NRAMP1 D543N G:G genotype and allele 3 (GT)n polymorphism had decreased recurrence time after BCG therapy. (Chiong et al., 2011)
Host Gene Response of GPX1
- Gene Response: Polymorphisms of the GPX1 gene may be associated with recurrence of BCa after BCG immunotherapy. (Chiong et al., 2011)
- Detailed Gene Information: Click here.
Host Gene Response of SLC11A1
- Gene Response: Polymorphisms of the SLC11A1 genes may be associated with recurrence of BCa after BCG immunotherapy. (Chiong et al., 2011)
- Detailed Gene Information: Click here.

j. Human Response

Vaccination Protocol: Intradermally vaccinated the infants within 48 hours of birth. Examination of whole blood stimulated ex vivo with 1.2×106 CFU BCG 10 weeks after newborn BCG vaccination of South African infants (Randhawa et al., 2011).
Immune Response: Increased production of TH1-type T cell cytokines (Randhawa et al., 2011).
Host Gene Response of GPX1
- Gene Response: After stimulation with TLR6 lipopeptide ligands, PBMCs from TLR6-deficient, BCG-vaccinated individuals secreted lower amounts of IL-6 and IL-10 compared to those with responsive TLR6 genotypes (Randhawa et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of human IFNG
- Gene Response: Increased IFNG production associated with TLR1/6 presence 10 weeks post BCG vaccination (Randhawa et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of IL10
- Gene Response: IL10 expression post vaccination increased in presence of TLR1/6 (Randhawa et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of IL2
- Gene Response: Increased IL2 production associated with TLR1/6 presence 10 weeks post BCG vaccination (Randhawa et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of IL6
- Gene Response: IL6 expression post vaccination expressed in higher amounts in presence of TLR1/6 (Randhawa et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of TLR1
- Gene Response: After stimulation with TLR1 lipopeptide ligands, PBMCs from TLR1-deficient, BCG-vaccinated individuals secreted lower amounts of IL-6 and IL-10 compared to those with responsive TLR1 genotypes (Randhawa et al., 2011).
- Detailed Gene Information: Click here.

k. Mouse Response

Host Gene Response of Ifng (Interferon gamma)
- Gene Response: Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of IFN-gamma in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of IL-6
- Gene Response: Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of IL-6 in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of Il2
- Gene Response: Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of IL-2 in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011).
- Detailed Gene Information: Click here.
Host Gene Response of TNF-alpha
- Gene Response: Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of TNF-alpha in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011).
- Detailed Gene Information: Click here.

l. Mouse Response

Host Strain: C57BL/6
Vaccination Protocol: Ten to twelve female C57BL/6 mice were immunized per group with 100 μl of the formulation, once, subcutaneously (s.c.) at the base of the tail. BCG and Lactoferrin were emulsified with Freund's adjuvant in a 1:1 ratio (Hwang et al., 2005).
Immune Response: A single immunization of mice with Lactoferrin as an adjunct adjuvant resulted in amplified splenocyte proliferative response to heat-killed BCG, and elevated IL-12(p40) production with increased relative ratios of IL-12/IL-10. Furthermore, splenocyte recall response to HK-BCG was augmented for proinflammatory mediators, TNF-alpha, IL-1beta, and IL-6, approaching responses generated to complete Freund's adjuvant (CFA) immunized controls (Hwang et al., 2005).
Challenge Protocol: Fourteen days post-immunization, four mice from each group were aerosol challenged with Erdman MTB (Hwang et al., 2005).
Efficacy: All immunization groups showed significant reduction in lung organism load, decreased bacterial load in the spleen (Hwang et al., 2005).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: Lactoferrin was able to significantly augment the production of IFN-γ compared to vaccine preparations in IFA alone (Hwang et al., 2005).
- Detailed Gene Information: Click here.
Host Gene Response of IL-1b
- Gene Response: In concert with the increased stimulation index, there was significant production of all three proinflammatory mediators (TNF-alpha, IL-1beta, and IL-6) in the Lactoferrin immunization group compared to both the non-immunized and IFA immunized groups (Hwang et al., 2005).
- Detailed Gene Information: Click here.
Host Gene Response of IL-6
- Gene Response: In concert with the increased stimulation index, there was significant production of all three proinflammatory mediators (TNF-alpha, IL-1beta, and IL-6) in the Lactoferrin immunization group compared to both the non-immunized and IFA immunized groups (Hwang et al., 2005).
- Detailed Gene Information: Click here.
Host Gene Response of Il12b
- Gene Response: A single administration of BCG in IFA and Lactoferrin resulted in significantly increased production of IL-12 in the splenic recall assay (Hwang et al., 2005).
- Detailed Gene Information: Click here.
Host Gene Response of Ltf
- Gene Response: Lactoferrin can act as an adjunct adjuvant to augment cellular immunity and boost BCG efficacy for protection against subsequent challenge with virulent MTB. Augmented responses were found for IL-12, TNF-alpha, IL-1beta, and IL-6 (Hwang et al., 2005).
- Detailed Gene Information: Click here.
Host Gene Response of TNF-alpha
- Gene Response: In concert with the increased stimulation index, there was significant production of all three proinflammatory mediators (TNF-alpha, IL-1beta, and IL-6) in the Lactoferrin immunization group compared to both the non-immunized and IFA immunized groups (Hwang et al., 2005).
- Detailed Gene Information: Click here.

m. Guinea pig Response

Host Strain: Dunkin Hartley (Thom et al., 2012).
Vaccination Protocol: Pathogen-free, female outbred Dunkin Hartley strain guinea pigs (weighing 500–550 g) were vaccinated subcutaneously with 5 × 104 colony forming units (CFU) of M. bovis BCG Pasteur (Thom et al., 2012).
Immune Response: Significant down-regulation of both ferritin light- and heavy-chain (Thom et al., 2012).
Description: PPD from M. tuberculosis was added to splenocytes from BCG-vaccinated guinea pigs at a final concentration of 30 μg/ml for 16 and 24 h at 37 °C in the presence of 5% CO2. The splenocyte cultures from naïve and BCG-vaccinated guinea pigs were infected with M. tuberculosis at an MOI of 0.2 and incubated for 4, 16 and 24 h at 37 °C in the presence of 5% CO2. The viability of uninfected and infected splenocytes from the same vaccination group was determined at 4, 16 and 24 h post-M. tuberculosis infection by trypan blue exclusion (Thom et al., 2012).
Host Gene Response of Fth1
- Gene Response: Splenocytes from BCG-vaccinated guinea pigs were stimulated ex vivo with purified protein derivative from M. tuberculosis and a significant down-regulation of ferritin light- and heavy-chain was measured by reverse-transcription quantitative-PCR (Thom et al., 2012).
- Detailed Gene Information: Click here.
Host Gene Response of Ftl
- Gene Response: Splenocytes from BCG-vaccinated guinea pigs were stimulated ex vivo with purified protein derivative from M. tuberculosis and a significant down-regulation of ferritin light- and heavy-chain was measured by reverse-transcription quantitative-PCR (Thom et al., 2012).
- Detailed Gene Information: Click here.

n. Rat Response

Host Strain: Wistar rat
Vaccination Protocol: Wistar rats were vaccinated with 10^6 BCG subcutaneously 6-8 weeks prior to challenge (Singhal et al., 2011).
Vaccine Immune Response Type: VO_0000286
Immune Response: A significantly greater number of total cells and CD8 T cells was observed in BCG vaccinated animals at day 60 post-challenge compared with unvaccinated animals. Immunohistochemistry revealed substantial higher number of CB8 cells in the lung lesions of vaccinated rats at day 30. Finally, a high number of mycobacteria-specific CD4 and CD8 T cells producing IFN-gamma was detected in BCG vaccinated rats (Singhal et al., 2011).
Challenge Protocol: The rats were challenged with a low dose of Mtb W4 strain 6 weeks after vaccination (Singhal et al., 2011).
Efficacy: A significantly reduced bacillary load was observed in the lungs of BCG vaccinated rats over the entire course of infection. About a 1 log(10) reduced bacillary load was observed in the lungs of vaccinated rats compared to unvaccinated animals (Singhal et al., 2011).
Description: The study showed that BCG vaccinated Wistar rats efficiently control early bacillary growth and pathology related to Mtb infection (Singhal et al., 2011).

o. Cattle Response

Host Strain: Friesian Calves
Vaccination Protocol: Vaccinated subcutaneously in the neck with 2 mL of 2X10^5 cfu BCG
Persistence: Cattle were examined 154 to 164 days after they had been challenged.
Vaccine Immune Response Type: VO_0000408
Immune Response: The cattle that had been vaccinated with BCG showed significantly less numbers of lesions in the lungs and lymph nodes.
Challenge Protocol: 54 days after vaccination the calves were challenged intratracheally with 2X10^3 cfu M. bovis strain 83/6235.
Efficacy: Vaccination with BCG induced considerable protection against a challenge with M. bovis, there were significantly fewer animals with Tuberculosis lesions.
Description: This study showed that killed M. vaccae did not protect against challenge by M. bovis, however, the vaccination of cattle with BCG by the respiratory route protected them against the development of tuberculous lung lesions.
Host Gene Response of IFNG
- Gene Response: In the vaccinated cattle group, increased IFN-gamma was released from the culture of peripheral blood lymphocytes from cattle after stimulation with bovine purified protein derivative (PPD) (Buddle et al., 1995).
- Detailed Gene Information: Click here.
Host Gene Response of IL10
- Gene Response: In the vaccinated cattle group, increased IL-2 was released from the culture of peripheral blood lymphocytes from cattle after stimulation with bovine purified protein derivative (PPD) (Buddle et al., 1995).
- Detailed Gene Information: Click here.

p. Macaque Response

Host Strain: cynomolgus monkey (M. fascicularis) and rhesus monkey (M. mulatta)
Vaccination Protocol: The macaques were vaccinated intradermally with 1–4 X 10^5 cfu bacillus Calmette–Guerin
Vaccine Immune Response Type: VO_0000859
Immune Response: Showed specific cell-mediated immune responses after bacillus Calmette–Guerin vaccination as observed by skin test, lymphoproliferation, and IFN-gamma production (Langermans et al., 2001).
Challenge Protocol: 17 weeks after vaccination the monkeys were challenged with 3,000 cfu M. tuberculosis by intratracheal installation of 3 ml of the bacterial suspension. Between 59 and 65 days after infection the animals were killed, and necropsies were undertaken for pathological examination (Langermans et al., 2001).
Description: This study showed that cynomolgus monkey (M. fascicularis) is a better model than rhesus monkey (M. mulatta) for M. tuberculosis vaccination and infection studies. From a challenge with 3,000 cfu M. tuberculosis, BCG vaccination protects cynomolgus monkeys with >2-log reduction of the bacterial load and diminished pathology, whereas only a minimal BCG vaccination effect was observed in rhesus monkeys. These two species represent the two extremes of BCG-induced protection that is found in humans (Langermans et al., 2001).

q. Rabbit Response

Host Strain: New Zealand White Rabbits
Vaccination Protocol: Rabbits were vaccinated three times at 3-week intervals intramuscularly with a total of 500 micro liters of BCG (Tsenova et al., 2006).
Persistence: Rabbits were euthanized 8 weeks post-infection (Tsenova et al., 2006).
Vaccine Immune Response Type: VO_0000134
Immune Response: Significantly smaller amounts of CFU of the bacterium were observed in the brains of rabbits that were vaccinated with BCG. No granulomas were found in the lungs of mice vaccinated with BCG while they were found in the unvaccinated group. Vaccination with BCG induced a statistically significant increase in antigen-specific T-cell proliferation compared to that of non-vaccinated controls (Tsenova et al., 2006).
Challenge Protocol: Ten weeks after immunization with BCG the rabbits were anesthetized and immobilized and a spinal needle was used to withdraw .3 mL of cerebrospinal fluid and .2 mL of 5X10^5 CFU of M. tuberculosis was injected intracisternally (Tsenova et al., 2006).
Efficacy: This study showed that vaccination with BCG induced protection against M. tuberculosis because rabbits that were vaccinated showed increased T-cell proliferation and no granulomas in the lungs (Tsenova et al., 2006).

r. Zebrafish Response

Host Strain: Wildtype AB or adult rag hu1999 mutant fish
Vaccination Protocol: Fish were vaccinated with ~8X10^3 CFU BCG intraperitoneally or intramuscularly (Oksanen et al., 2013).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: Fish were anesthetized and intraperitoneally injected 3-4 weeks after immunization with ~20-30 bacteria (Oksanen et al., 2013).
Efficacy: BCG was unable to prevent infection with mycobacteria, however, it improved fish survival lowering the mortality rate from 84.2% to 45.0% (Oksanen et al., 2013).
Description: BCG is unable to prevent infection in zebrafish, however, it is able to improve fish survival (Oksanen et al., 2013).

5. BCG auxotroph expressing HIV-1 clade immunogen

a. Vaccine Ontology ID:

VO_0004122

b. Type:

Live, attenuated vaccine

c. Antigen

HIVA, an African HIV-1-derived immunogen, was identified as stable and inductive of HIV-1-specific CD4+ and CD8+ T cell responses. HIVA in this study contained an immunodominant H-2D^d restricted epitope P18-I10 (Im et al., 2007).

d. Preparation

HIVA gene with mAb tag Pk was fused to M. tuberculosis 19-kDa lipoprotein signal sequence via PCR to improve foreign protein immunogenicity. HIVA was cloned into two plasmids, pJH222 and pJH223, as HindIII-HindIII fragment and used to transform a lysine auxotroph of BCG (BCG Pasteur lysA5::res). Additional preparations included MVA.HIVA and BCG.p. BCG cultures were grown in presence of kanamycin. HIVA immunogenicity was assessed using H-2D^d-restricted epitope P18-I10 and subdominant H-2K^d-restricted P epitope. MHC class II-restricted responses were tested using three epitope peptides derived from p24 (Im et al., 2007).

e. Description

Goal of study was to develop a vaccine to minimize the risk of mother-to-child transmission (MTCT) of M. tuberculosis and HIV-1, and to protect against both pathogens in newborns. BCG was selected as a vaccine vector due to its long efficacy as a vaccine (10-15 years), frequent use in infant and newborn vaccination, and induction of humoral and cellular responses (Im et al., 2007).

f. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: Mice were immunized on day 0 with either BCG.HIVA (pJH222.HIVA) and episomal plasmid, parental BCG (BCG.p), or control (no immunization). Half of the animals were boosted with MVA.HIVA on day 102. Mice were sacrificed on day 151. T cells were stimulated with peptides in a multicolor flow cytometric analysis to elicite interferon gamma, tumor necrosis factor alpha, and degranulating responses (Im et al., 2007).
Immune Response: HIVA-specific responses peaked after 12 weeks, most likely as a result of BCG-induced activation of T cells. Three major observations were observed:
1) BCG.HIVA alone induces undetectable CD8+ HIV-1-specific T cell responses secreting tum, though priming with BCG.HIVA shows significant increases in elicited H and P-specific splenocytes producing IFN-gamma and degranulating. Hence, BCG.HIVA enhancement of MVA.HIVA responses is HIVA-specific.
2) BCG.HIVA priming with MVA.HIVA boost elicits the highest proportion of HIV-1 specific bifunctional cells in all the studies.
3) No HIVA-induced CD4+ T cell responses were detected (Im et al., 2007).

g. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: Mice were primed with increasing doses (10^3-10^7 CFU/animal, day 0) of BCG.HIVA or not vaccinated, and boosted (day 102) with constant doses of MVA.HIVA.
Immune Response: Bifunctional responses increased steadily with dose up to 0.7% CD8+ splenocytes with 10^6 CFU BCG.HIVA for epitope H , and increased up to 0.2% CD8+ with 10^7 CFU for P response (Im et al., 2007). Priming dose of BCG.HIVA affects strength and quality of CD8+ T cell responses induced by the recombinant BCG priming followed by recombinant MVA boosting, with high priming dose being most important for quality of response to P epitope (Im et al., 2007).

h. Mouse Response

Host Strain: BALB/c, 6-8 wk old
Vaccination Protocol: Pathogen free mice were vaccinated in groups of 7-9 animals with 3x10^5 CFU of BCG.HIVA, BCG.p, or BCG wild type 1173 p2 via s.c. injection into left hind foot.
Challenge Protocol: Mice were challenged via aerosol (nasal) route 12 weeks post-vaccination with M. tuberculosis Erdman using a modified Henderson apparatus. Approximately 25 CFU/lung were estimated 24 hours post-challenge (Im et al., 2007).
Efficacy: All three vaccines tested here showed an approximate 2-fold reduction in M. tuberculosis load in the lungs and spleen. Data suggest that the "safer" lysine auxotrophBCG.HIVA could replace BCG vaccine in neonatal uses and still show comparable efficacy (Im et al., 2007).

i. Mouse Response

Host Strain: BALB/c, female
Vaccination Protocol: Groups of 4-5 mice were naive or immunized with 100 μg pTHr.HIVA, 10^6 pfu MVA.HIVA, or 10^6 cfu of either BCG.p or BCG.HIVA. BCG.HIVA boosting commenced on day 33.
Immune Response: No HIVA-specific responses were noticed during the 4-day WR.HIVA infection. Mice receiving the pTHr.HIVA-BCG.p, pTHr.HIVA-BCG.HIVA , or BCG.HIVA showed both H and P specific responses. Highest responses were seen in pTHr.HIVA-BCG.HIVA-WR.HIVA which showed interferon gamma and degranulated responses. CD4+IFN-gamma+TNF-alpha+IL-2+ splenocytes exhibited high quality characteristics in the BCG.HIVA and BCG.p boosts following pTHr.HIVA priming. HIVA non-specific mechanisms were suggested by a two-log10 decrease in WR.HIVA titre when BCG.HIVA or BCG.p alone were used in immunization (Im et al., 2007).
Challenge Protocol: Mice were challenged i.p. using 4x10^6 pfu vaccinia virus WR.HIVA. Plaque counts were determined for sonicated and serially-diluted mouse ovaries four days post-challenge (Im et al., 2007).
Description: Surrogate challenge with recombinant replication-competent vaccinia virus (strain Western Reserve) is a cost-effective murine alternative to HIV-1challenge in non-human primate models.

6. BCG expressing MSP

a. Vaccine Ontology ID:

VO_0004107

b. Type:

Live, attenuated vaccine

c. Antigen

MSP, a 30-kDa major secretory protein or mycolyltransferase, is used as vaccine antigen (Horwitz et al., 2000).

d. Preparation

Recombinant BCG (rBCG30) was prepared using E.coli/mycobacteria shuttle plasmid pSMT3. Plasmid was electoporated into the wild-type M. bovis BCG Tice strain. The 30-kDa MSP was isolated from the subcultured strains and further processed prior to inclusion in the vaccine (Horwitz et al., 2000).

e. Guinea pig Response

Host Strain: Hartley, 250-350g outbred male
Challenge Protocol: Animals were challenged nine weeks after first injection or skin testing via aerosol with 10-ml single-cell suspension containing 1x10^5 CFU (1st & 2nd experiment) or 2x10^5 CFU (3rd experiment) of M. tuberculosis Erdman, providing ~40 to 80 liver bacilli to lungs, respectively (Horwitz et al., 2000).
Efficacy: The highly susceptible guinea pig model of pulmonary TB, a model noteworthy for its close resemblance to human TB, was used for testing. Animals immunized with the recombinant BCG vaccine and challenged by aerosol with a highly virulent strain of M. tb. had 0.5 log fewer M. tb. bacilli in their lungs and 1 log fewer bacilli in their spleens on average than animals immunized with their parental conventional BCG vaccine counterparts. Statistically, the difference was highly significant. At necropsy, animals immunized with the recombinant BCG vaccine had fewer and smaller lesions in the lung, spleen, and liver and significantly less lung pathology than animals immunized with the parental BCG vaccine (Horwitz et al., 2000).

7. BCG mutant secreting listeriolysin

a. Vaccine Ontology ID:

VO_0000603

b. Type:

Subunit vaccine

c. Antigen

The tuberculosis vaccine Mycobacterium bovis bacille Calmette-Guérin (BCG) was equipped with the membrane-perforating listeriolysin (Hly) of Listeria monocytogenes (Grode et al., 2005).

d. Preparation

BCG pAT261:Hly and BCG pMV306:Hly with actively-secreted hemolysin were constructed using chromosomal integration of a mycobacterial shuttle vector (pAT261 or PMV306) or by episomal plasmid replication, respectively (Hess et al., 1998; Hess et al., 1999). These plasmids were introduced into M. bovis BCG using standard electroporation. Fusion proteins were isolated and separated (Hess et al., 1998). In later studies, the fusion proteins were expressed by S. typhimurium strain SL7207's aroA pathway (Hess et al., 1999).

e. Virulence

The r-BCG has decreased intracellular persistence versus the parental BCG strain (Hess et al., 1999). Mycobacterial CFUs from infected macrophages were used to ascertain survival of BCG pAT261:Hly and BCG pMV306:Hly in host macrophages. Intracelluar survival of the BCG pMV306:Hly strain was reduced at day 1 post-infection, and viable BCG pAT261:Hly were not detected in J774A.1 macrophages at 15 days post-infection. Furthermore, the recombinant strains failed to escape from the phagosome of macrophages (Hess et al., 1998).

f. Description

A recombinant BCG strain was constructed to secrete a pore-forming sulfhydrul-activated cytolysin (listeriolysin or Hly) from Listeria monocytogenes. Listeriolysin assists Listeria in escaping from the phagosome into the cytosol of the infected host cell. The study shows that Hly secretion improves stimulation of CD8 T cells (by pronounced MHC class I processing and delivery) in recombinant BCG (Hess et al., 1999).

8. BCG Vaccine

a. Product Name:

BCG Live

b. Tradename:

BCG Vaccine

c. Manufacturer:

Organon Teknika Corp., LLC

d. Type:

Live, attenuated vaccine

e. Status:

Licensed

f. Location Licensed:

USA

g. Host Species for Licensed Use:

Human

h. Immunization Route

Percutaneous

i. Storage

Stored at 2–8°C (36–46°F), protect from sunlight.

j . Approved Age for Licensed Use

All ages.

k. Description

BCG VACCINE for percutaneous use, is an attenuated, live culture preparation of the Bacillus of Calmette and Guerin (BCG) strain of Mycobacterium bovis. The TICE® strain used in this BCG VACCINE preparation was developed at the University of Illinois from a strain originated at the Pasteur Institute. (FDA: BCG Vaccine)

9. BCG Vaccine (Freeze-Dried)

a. Product Name:

BCG Vaccine (Freeze-Dried)

b. Manufacturer:

Sanofi Pasteur Ltd

c. Vaccine Ontology ID:

VO_0010708

d. Type:

Live, attenuated vaccine

e. Status:

Licensed

f. Location Licensed:

Canada

g. Host Species for Licensed Use:

Human

h. Immunization Route

Intradermal injection (i.d.)

i. Description

Products: Live bacteria. Other components: Polysorbate 80.

10. BCG-DHTM

a. Vaccine Ontology ID:

VO_0004618

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Antigen

BCG vaccine with deficient urease; a fusion protein composed of Mycobacterium tuberculosis-derived major membrane protein II (MMP-II) and heat shock protein 70 (HSP70) of BCG (Mukai et al., 2014).

e. Gene Engineering of DnaK

Type: Recombinant vector construction
Description: The M. tuberculosis gene dnaK, also known as hsp70, is a part of a fusion gene composed of the M. tuberculosis-derived MMP-II gene and the hsp70 gene of M. tuberculosis (locus tag: Rv0350). This fusion gene is introduced into the urease-deficient BCG-deltaUT-11-3 strain (Mukai et al., 2014).
Detailed Gene Information: Click here.

f. Gene Engineering of bfrA

Type: Recombinant vector construction
Description: The M. tuberculosis gene bfrA (also known as MMP-II) is a part of a fusion gene composed of the M. tuberculosis-derived MMP-II gene and the hsp70 gene of M. tuberculosis (locus tag: Rv0350). This fusion gene is introduced into the urease-deficient BCG-deltaUT-11-3 strain (Mukai et al., 2014).
Detailed Gene Information: Click here.

g. Immunization Route

subcutaneous injection

h. Mouse Response

Host Strain: C57BL/6J
Vaccination Protocol: Five mice per group were vaccinated with BCG-261H (control) or BCG-DHTM at 1000 CFU/mouse for 6 weeks (Mukai et al., 2014)
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: Mice were challenged with H37RV at 100 CFU per lung by aerosol infection (Mukai et al., 2014)
Efficacy: At 6 weeks post challenge, mice vaccinated with BCG-261H (control) or BCG-DHTM showed inhibited multiplication of M. tuberculosis in the lung, and BCG-DHTM inhibited M. tuberculosis multiplication more strongly than BCG-261H (Mukai et al., 2014).

11. DNA vaccine co-expressing A85A and caspase-3

a. Vaccine Ontology ID:

VO_0004148

b. Type:

DNA vaccine

c. Antigen

The antigens for this vaccine were wild-type or mutated caspase-3 inserted into a plasmid that encodes mycolyl transferase antigen 85A (Ag85A) (Gartner et al., 2008).

d. Gene Engineering of FbpA (Ag85A)

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

e. Vector:

pBudCE4.1 (Gartner et al., 2008)

f. Preparation

Native Ag85A was purified from BCG culure and recombinant his-tagged Ag85A was purified by affinity chromatography. Dual expression-vectors were created encoding mature or secreted Ag85A and either mutant or wild-type caspase-3 (Gartner et al., 2008).

g. Mouse Response

Host Strain: (C57BL/6 × DBA/2)F1 (H-2b/d) or BALB/c (H-2d)
Vaccination Protocol: Mice were injected four times intramuscularly with 100 micrograms of pDNA with at least three weeks between injections. As negative controls, mice were injected with 100 micrograms of empty pBudCE4.1 vector or left untreated. As a positive control for the challenge experiments, mice were vaccinated intravenously with M. bovis BCG strain GL2 along with the first vaccination or 5 weeks pre-vaccination (Gartner et al., 2008).
Immune Response: Ag85A specific antibody titers were very low in mice vaccinated with the wild-type p-sAg85A–csp, but antibody titers in mice vaccinated with mutant p-sAg85A–csp were of a greater magnitude. IFN-γ and IL-2 production in mice vaccinated with plasmids co-expressing mAg85A and a caspase gene were similar to or lower than production in mice vaccinated with p-sAg85A. Vaccination with the wild-type caspase-encoding plasmids was not effective in inducing Ag85A specific CTL activity (Gartner et al., 2008).
Challenge Protocol: Mice were challenged five weeks after their final vaccination with 2 x 10^5 CFUof M. tuberculosis H37Rv. Results were measured using a luminescence assay with a count of relative light units (RLU) (Gartner et al., 2008).
Efficacy: The challenge revealed that mice vaccinated with mutant p-sAg85A–csp showed higher secretion of IL-6, IL-10 and IL-17A in their lungs, spontaneously and/or after antigenic stimulation.However, spontaneous IFN-γ production was highest in mice vaccinated with wild-type p-sAg85A–csp. Ag85A specific IL-2 and IFN-γ responses were low in spleen of BCG vaccinated mice, but high Th1 responses could be could be seen after stimulation with whole BCG culture filtrate in these mice (Gartner et al., 2008).

12. DNA vaccine Rv1806-1807

a. Vaccine Ontology ID:

VO_0000567

b. Type:

DNA vaccine

c. Antigen

Rv1806 and Rv1807 (Vipond et al., 2006).

d. Gene Engineering of PE20

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

e. Gene Engineering of PPE31

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0001261
Description: Dioctadecylammonium (DDA), trehalose dibehenate (TDB), and stable liposomes (SSI) (Vipond et al., 2006)

g. Vector:

pET11d, modified to form pET3a (Vipond et al., 2006)

h. Preparation

Two bacterial strains, E. coli DH5α and Rosetta™ 2(DE3)pLysS, were propagated in Luria Bertani (LB) broth and solid medium at 37°C. An expression vector pET3a was created from pET11d expression vector (Novagen) by replacing an ampicillin cassette with kanamycin cassette, followed by restriction digestion of a HindIII site and linker insertion containing cloning sites and an N- and C-terminal 6* histidine tag. The vaccine candidate gene was amplified from M. tuberculosis using PCR and cloned into pVAX1tPA. Recombinant plasmids were generated from four encoding gene sequences and sub-cloned into pET3a prior to transformation, induction, and harvesting in E. coli Rosetta™ 2(DE3)pLysS (Vipond et al., 2006).

i. Virulence

Not virulent.

j. Description

Rv1806-1807 was one of three potential vaccines compared against gold-standard BCG vaccine and saline control. Rv1806-1807 induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M tuberculosis in the lungs ( a reduction in log10 cfu of 0 . 52 and 0 . 83 in lungs and spleen , respectively , following DNA vaccination , compared with reductions of 0 . 3 and 0 . 57 , respectively , with a protein formulation) (Vipond et al., 2006).

k. Guinea pig Response

Host Strain: Dunkin-Hartley guinea pig
Vaccination Protocol: Female Dunkin-Hartley guinea pigs were vaccinated by injecting 100 μg of protein intramuscularly into both hind quadriceps muscles per guinea pig in 100 μl of dioctadecylammonium (DDA) and trehalose dibehenate (TDB) (500 μg DDA plus 100 μg TDB) stable liposomes (SSI). Sub-cutaneous inoculation with 5×10^4 CFU BCG Pasteur was used as positive control. Saline was used as negative control. Ten guinea pigs were used for each group. Vaccinated animals were booster vaccinated with 200 μg protein in adjuvant after 3 and 6 weeks (Vipond et al., 2006).
Challenge Protocol: Six weeks after the final vaccination, six animals per group were aerosol challenged with approximately 50 CFU of M. tuberculosis H37Rv (NCTC 7416) each in a contained Henderson apparatus. Protection was assessed by CFU in the lungs and spleen at 30 days post-challenge (Vipond et al., 2006).
Efficacy: Level of protection by Rv1806-1807 at week 15 post challenge was significantly better than saline (p=.018) and comparable to related DNA vaccinations. However, the protection was significantly less than gold-standard BCG. An antigen dose of 10 μg/ml of Rv1806-1807 vaccine stimulated the better protection than for 1 μg/ml (Vipond et al., 2006).

13. ID93/GLA-SE

a. Type:

Subunit vaccine

b. Status:

Clinical trial

c. Host Species for Licensed Use:

Baboon

d. Gene Engineering of Rv1813c

Type: Recombinant protein preparation
Description: D93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010)
Detailed Gene Information: Click here.

e. Gene Engineering of esxV

Type: Recombinant protein preparation
Description: ID93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010)
Detailed Gene Information: Click here.

f. Gene Engineering of esxW

Type: Recombinant protein preparation
Description: ID93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010)
Detailed Gene Information: Click here.

g. Gene Engineering of PPE42

Type: Recombinant protein preparation
Description: ID93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010)
Detailed Gene Information: Click here.

h. Immunization Route

Intramuscular injection (i.m.)

14. ΔureC hly+ rBCG

a. Vaccine Ontology ID:

VO_0000606

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Preparation

In order to generate a live vaccine that provides improved protection against M. tuberculosis, BCG was equipped with the membrane-perforating Hly of L. monocytogenes (hly+ rBCG). To improve the pH milieu for Hly activity, a ΔureC hly+ rBCG strain that lacked ureC activity was constructed (Grode et al., 2005).

e. Immunization Route

Intramuscular injection (i.m.)

f. Cattle Response

Vaccination Protocol: Mice were immunized i.v. with 10^6 CFU of BCG strains (Grode et al., 2005).
Challenge Protocol: At 120 days after vaccination, animals were aerosol challenged with 100–200 CFU of M. tuberculosis H37Rv or Beijing/W per lung using an aerosol chamber (Glas-Col) (Grode et al., 2005).
Efficacy: The ΔureC hly+ rBCG vaccine strain induced significantly more efficacious in inducing protective immunity against 2 different M. tuberculosis strains, the laboratory strain H37Rv and a clinical isolate of the Beijing/W genotype family, in a mouse model, than parental BCG (Grode et al., 2005). The ΔureC hly+ rBCG vaccine provided even more efficient protection than the hly+ rBCG vaccine strain.

15. LT-BCG- Ag85B /Rv3425

a. Vaccine Ontology ID:

VO_0004626

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Gene Engineering of Ag85B from M. tuberculosis H37Rv

Type: Recombinant vector construction
Description: DNA-, protein- and lentiviral vector-based vaccines that express the antigens Ag85B and Rv3425 (Xu et al., 2014).
Detailed Gene Information: Click here.

f. Vector:

(Xu et al., 2014)

g. Preparation

DNA-, protein- and lentiviral vector-based vaccines that express the antigens Ag85B and Rv3425 (Xu et al., 2014).

h. Immunization Route

Intramuscular injection (i.m.)

i. Mouse Response

Host Strain: C57BL/6
Vaccination Protocol: Six mice per group were first immunized subcutaneously (s.c.) with 5×10^6 CFU of BCG in 100μl of PBS and subsequently boosted twice at 2-week intervals with PAR, DAR or LAR [12]. Meanwhile, the mice were boosted with the controls for these three types of vaccines, which were PNC (i.e., MPL + TDM), DNC (i.e., pVax) and LNC (i.e.,pLenti6.3). For DAR immunization, 50μg of the DAR DNA vaccine in 100μl of sterile PBS was administered to the mice intramuscularly (i.m.) in the right thigh. For LAR immunization, the mice were immunized with 5×10^6 pfu of LAR in the foot pad. For PAR immunization, the mice were immunized subcutaneously (s.c.) with 50 μg of PAR formulated with the adjuvants MPL and TDM (Xu et al., 2014).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: 2 weeks after the last vaccination, all mice were challenged intravenously via the lateral tail vein with 1.2 × 10^6 CFU of M. tuberculosis H37Rv (Xu et al., 2014).
Efficacy: Prime-boost BCG vaccination with a lentiviral vector expressing the antigens Ag85B and Rv3425 significantly enhanced immune responses, including Th1 and CD8+ CTL responses, compared to DNA- and protein-based vaccines. However, lentivirus-vectored and DNA-based vaccines greatly improved the protective efficacy of BCG against M. tuberculosis (Xu et al., 2014).

16. M. bovis WAg520 strain

a. Vaccine Ontology ID:

VO_0000552

b. Type:

Attenuated vaccine

c. Antigen

The antigen for this vaccine was an attenuated strain of M. bovis called WAg520, which cantains a deletion in a possible undecaprenol kinase gene (De et al., 1999).

d. Preparation

WAg520 was one of four nutritionally impaired strains of M. bovis selected from 440 M. bovis mutants produced by illegitimate recombination based upon their inability to grow in minimal medium (De et al., 1999).

e. Virulence

This strain, along with the other test M. bovis mutants, was shown to be avirulent for guinea pigs following the subcutaneous inoculation (De et al., 1999).

f. Description

WAg520, an attenuated strain of M. bovis, has only a 2-bp deletion in a possible undecaprenol kinase gene. It is one of two nutritionally impaired strains which produced significant protection in the lungs. The other one is strain WAg522 (De et al., 1999).

g. Guinea pig Response

Host Strain: Duncan-Hartley
Vaccination Protocol: Four to eight guinea pigs per group were vaccinated by subcutaneous injection with 10^5 CFU with one of the four impaired M. bovis strains or BCG. Ten animals were not vaccinated (control) (De et al., 1999).
Side Effects: No delayed-type hypersensitivity reactions observed prior to inoculation with mycobacteria (De et al., 1999). Authors were unable to determine if nutritionally-impaired mutants influenced the development of necrotizing lesions in the lungs (De et al., 1999).
Challenge Protocol: All animals were anesthetized eight weeks post-vaccination via intramuscular injection with 3 mg ketamine hydrochloride, followed by 10x2 CFU virulent M. bovis WAg201 introduced through mouth into the trachea via cannula. Three guinea pigs which were not challeged received 10^5 CFU of each of the four strains via subcutaneous innoculation (De et al., 1999).
Efficacy: Significant differences in lung CFU were observed between vaccinated groups and WAg520 & WAg522 strains (De et al., 1999). WAg520 elicited 0.92, 3.83, and 2.47 log10 increases in lung, splenic, and liver tissues, respectively (Table I, (De et al., 1999)). Macroscopic lesions were decreased in WAg520 recipients versus both non-vaccinated (significant different) and BCG-vaccinated (not significantly different) animals (Table 2, (De et al., 1999)).
Description: All animals were sacrificed 13 weeks post-vaccination.

17. M. bovis WAg522 strain

a. Vaccine Ontology ID:

VO_0000557

b. Type:

Attenuated vaccine

c. Antigen

The antigen for this vaccine was an attenuated strain of M. bovis called WAg522 (De et al., 1999).

d. Preparation

WAg522 is the second of four M. bovis mutants selected from 440 illegitimate recombinant M. bovis mutants via their inability to grow in minimal medium (De et al., 1999).

e. Virulence

This strain, along with the other tested M. bovis mutants in (De et al., 1999), was shown to be avirulent in guinea pigs following subcutaneous inoculation. Furthermore, the groups treated with WAg522 and WAg520 strains both showed significant differences in lung CFU versus nonvaccinated groups.

f. Guinea pig Response

Host Strain: Duncan-Hartley
Vaccination Protocol: Four to eight guinea pigs per group were vaccinated by subcutaneous injection with 10^5 CFU with one of the four impaired M. bovis strains or BCG. Ten animals were not vaccinated (control) (De et al., 1999).
Side Effects: No delayed-type hypersensitivity reactions observed prior to inoculation with mycobacteria (De et al., 1999). Authors were unable to determine if nutritionally-impaired mutants influenced the development of necrotizing lesions in the lungs (De et al., 1999).
Challenge Protocol: All animals were anesthetized eight weeks post-vaccination via intramuscular injection with 3 mg ketamine hydrochloride, followed by 10x2 CFU virulent M. bovis WAg201 introduced through mouth into the trachea via cannula. Three guinea pigs which were not challeged received 10^5 CFU of each of the four strains via subcutaneous innoculation (De et al., 1999).
Efficacy: Significant differences in lung CFU were observed between vaccinated groups and WAg520 & WAg522 strains (De et al., 1999). WAg520 elicited 0.92, 3.83, and 2.47 log10 increases in lung, splenic, and liver tissues, respectively (see Table I, (De et al., 1999)). Macroscopic lesions were decreased in WAg520 recipients versus both non-vaccinated (significant different) and BCG-vaccinated (not significantly different) animals (see Table 2, (De et al., 1999)).

18. M. tuberculosis DNA vaccine (containing the ESAT-6, MPT-64, MPT-83, and KatG constructs)

a. Vaccine Ontology ID:

VO_0004363

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of MPT64

Type: DNA vaccine construction
Description: Vector pJW4303 encoded MPT-64 (Morris et al., 2000).
Detailed Gene Information: Click here.

f. Gene Engineering of MPT83

Type: DNA vaccine construction
Description: Vector pJW4303 encoded MPT-83 (Morris et al., 2000).
Detailed Gene Information: Click here.

g. Gene Engineering of KatG

Type: DNA vaccine construction
Description: Vector pJW4303 encoded KatG (Morris et al., 2000).
Detailed Gene Information: Click here.

h. Vector:

pJW4303 (Morris et al., 2000)

i. Immunization Route

Intramuscular injection (i.m.)

j. Mouse Response

Vaccine Immune Response Type: VO_0000286
Efficacy: The relative protection in the lung induced by the combination vaccine approached the BCG response in these experiments (Morris et al., 2000).

19. M. tuberculosis DNA Vaccine APADNA priming and APAMVA boosting

a. Vaccine Ontology ID:

VO_0011478

b. Type:

Other

c. Status:

Research

d. Gene Engineering of Apa

Type: Recombinant vector construction
Description:
Detailed Gene Information: Click here.

e. Vector:

pUMVC3 and poxvirus (Kumar et al., 2003).

f. Immunization Route

Intradermal injection (i.d.)

g. Guinea pig Response

Vaccination Protocol: Animals were immunized intradermally with APADNA, 200 μg, given three times, 4 weeks apart (at weeks 0, 4, and 8), followed by APAMVA, 10^7 PFU, given 4 weeks after the third DNA (at 12 weeks); or BCG Guindy, 10^6 CFU, given one time only (at week 0) or control DNA, 200 μg, given three times, 4 weeks apart (at weeks 0, 4, and 8), followed by MVA wild type, 10^7 PFU, given 4 weeks after the third DNA (at week 12) (Kumar et al., 2003).
Challenge Protocol: Animals were challenged intramuscularly in the thigh muscle at 4 weeks after the last immunization with 2 × 10^5 viable M. tuberculosis strain NTI64719. This challenge dose was sufficient to cause measurable clinical illness in all control animals within a relatively short time frame of 6 weeks (Kumar et al., 2003).
Efficacy: A poxvirus recombinant expressing the Apa protein conferred a significant level of protective immunity in guinea pigs against a challenge dose of virulent M. tuberculosis (Kumar et al., 2003).

20. M. tuberculosis DNA vaccine DNAAg85A encoding a single immunogenic M.tb Ag

a. Vaccine Ontology ID:

VO_0004364

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of FbpA (Ag85A)

Type: DNA vaccine construction
Description: Vector pcDNA3.1 expressed a single immunogenic M.tb Ag (Ag85A) (Wang et al., 2004).
Detailed Gene Information: Click here.

f. Vector:

pCDNA3.1 (Wang et al., 2004)

g. Immunization Route

intranasal immunization

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Efficacy: Systemic priming with two repeated DNAAg85A injections and respiratory mucosal boosting once with AdAg85A provided the most potent protection of all of the tested vaccines/regimens, including s.c. BCG vaccination both at 4 and 8 wk postchallenge, and this level of protection was also better than single i.n. AdAg85A vaccination at 8 wk postchallenge (Wang et al., 2004).

21. M. tuberculosis DNA Vaccine encoding Ag85B Protein

a. Vaccine Ontology ID:

VO_0011410

b. Type:

DNA vaccine

c. Status:

Research

d. Gene Engineering of Ag85B from M. tuberculosis H37Rv

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

e. Vector:

pRC (Palma et al., 2007)

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Host Strain: C57BL/6
Vaccination Protocol: Mice at or between 7 and 8 weeks old were immunized. Fifty micrograms of plasmid, both DNA-85B or control vector, was injected intramuscularly (i.m.) in 50 μl PBS into the hind leg. Mice were immunized two or four times at 2 week intervals. As a positive control, a single dose of BCG (105 cfu) was injected s.c. (Palma et al., 2007).
Challenge Protocol: Four weeks after the last boost, mice were challenged intravenously (i.v.) in a lateral tail vein with 10^5 cfu of MTB H37Rv (Palma et al., 2007).
Efficacy: A significant reduction of the bacterial load was observed in mice receiving two administrations of the DNA-85B vaccine (DNAx2-immunized mice) with respect to unvaccinated naïve control or empty-plasmid DNA recipient mice, indicating protection by the vaccine. The degree of reduction of the mycobacterial burden was not significantly different from that conferred by BCG, although the immunization with BCG caused the highest nominal reduction of mycobacteria in the lungs (Palma et al., 2007).

22. M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83

a. Vaccine Ontology ID:

VO_0004362

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of Ag85B from M. tuberculosis H37Rv

Type: DNA vaccine construction
Description: Vector pJW4303 expressed Ag85B of H37Rv (Cai et al., 2005).
Detailed Gene Information: Click here.

f. Gene Engineering of MPT64

Type: DNA vaccine construction
Description: Vector pJW4303 expressed MPT64 of H37Rv (Cai et al., 2005)
Detailed Gene Information: Click here.

g. Gene Engineering of MPT83

Type: DNA vaccine construction
Description: Vector pJW4303 expressed MPT83 of H37Rv (Cai et al., 2005).
Detailed Gene Information: Click here.

h. Vector:

pJW4303 (Cai et al., 2005)

i. Immunization Route

Intramuscular injection (i.m.)

j. Mouse Response

Vaccine Immune Response Type: VO_0000286
Efficacy: Combined DNA vaccines including Ag85B, MPT64, and MPT83 formulated in IL-2 provided an efficient method for generating strong and sustained protective immunity in the spleen and lungs of experimental mice following i.v. challenge with M. tuberculosis H37Rv (Cai et al., 2005).

23. M. tuberculosis DNA Vaccine encoding KatG

a. Vaccine Ontology ID:

VO_0004058

b. Type:

DNA vaccine

c. Status:

Research

d. Gene Engineering of KatG

Type: DNA vaccine construction
Description: KatG protein was amplified from M. tuberculosis H37Rv chromosomal DNA by PCR, and then cloned into the eukaryotic expression vector pJW4303 (Li et al., 1999).
Detailed Gene Information: Click here.

e. Vector:

The eukaryotic expression vector pJW4303

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Host Strain: C57BL/6
Vaccination Protocol: The C57BL/6 mice (five mice per group) were vaccinated intramuscularly into the thigh three times at 3-week intervals, with 100 μg of plasmid DNA per injection. Control mice were vaccinated with 10^6 CFU of BCG Pasteur injected subcutaneously (Li et al., 1999).
Challenge Protocol: The mice were challenged by the aerosol route about 17 to 21 days after receiving the final DNA vaccine inoculation and 6 weeks after receiving the BCG vaccination with M. tuberculosis Erdman (Li et al., 1999).
Efficacy: Vaccination with the KatG resulted in a 75 to 85% drop in number of lung bacteria. Moreover, mice immunized with the KatG TPA-positive constructs showed significantly decreased dissemination of the lung infection to the spleen (Li et al., 1999).

24. M. tuberculosis DNA vaccine ESAT-6

a. Vaccine Ontology ID:

VO_0004035

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Vector:

pJW4303 (Morris et al., 2000)

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Vaccine Immune Response Type: VO_0000286
Immune Response: Immunization with a multivalent combination DNA vaccine (containing the ESAT-6, MPT-64, MPT-63, and KatG constructs) generated immune responses that indicated an absence of antigenic competition since antigen-specific cell-mediated and humoral responses were detected to each component of the mixture (Morris et al., 2000).
Efficacy: Prior to vaccination, mice were challenged with M. tuberculosis. Among the single DNA vaccines that were tested, the ESAT-6 construct consistently evoked the best protective responses. Compared with BCG, which on a relative scale elicited a protective value of 1, ESAT-6 elicited a protective value of 0.61 (Morris et al., 2000).

25. M. tuberculosis DNA vaccine pAK4-sod

a. Vaccine Ontology ID:

VO_0004123

b. Type:

DNA vaccine

c. Antigen

mycobacterial antigen superoxide dismutase A (Rv3846).

d. Gene Engineering of SodA

Type: DNA vaccine preparation
Description: The gene sodA was subcloned into the expression vectors pAK3 and pAK4 (Khera et al., 2005).
Detailed Gene Information: Click here.

e. Vector:

pAK4 (Khera et al., 2005)

f. Preparation

PCR amplified using gene specific primers containing Bgl II overhangs and M. tuberculosis genomic DNA as template. The blunt end PCR amplicon was cloned into EcoR V digested pLitmus-38, and then subcloned into plasmids pAK3 and pAK4 resulting in pAK3-sod and pAK4-sod. While both pAK3 and pAK4 are the eukaryotic expression vectors, pAK4 contains more CpG motifs. pAK4 also carries the ampicillin resistance gene carrying two immunostimulatory sequences. pAK3 is devoid of this ampicillin gene (Khera et al., 2005).

g. Virulence

None

h. Description

A study compared DNA vaccines expressing mycobacterial antigens ESAT-6 (Rv3875), α-crystallin (Rv2031c) and superoxide dismutase A (Rv3846) in Balb/c mice and guinea pigs. The DNA vaccine expression sodA was found to offer maximum protection (Khera et al., 2005).

i. Mouse Response

Host Strain: Balb/c mice
Vaccination Protocol: Mice (in groups of six each) were immunized with either the pAK3 or pAK4 vector controls or DNA vaccine candidates. Three doses of 100 μg of plasmid DNA were administered intramuscularly at 3-week intervals. Following a rest period of 3 weeks after the last dose, mice were euthanized, and antigen specific cell mediated and humoral immune responses were evaluated (Khera et al., 2005).
Immune Response: Immunization of mice with the DNA vaccines expressing superoxide dismutase A resulted in markedly higher levels of IFN-γ as compared to the levels of IL-10. The levels of IFN-γ in supernatants from pAK3-sod and pAK4-sod immunized mice increased 2.9 and 9.1 folds compared to the levels of IFN-γ in culture supernatant of mice immunized with pAK-3 and pAK-4, respectively. Approximately 3-fold increase in IFN-γ levels in pAK4-sod immunized mice in comparison to pAK3-sod immunized mice showed that the extra CpG motif activated Th1 subtype of host immunity. Though expression of SOD antigen increases IL-10 levels, the extra CpG motifs in the vector backbone decreases the concentration of IL-10 in pAK4-sod immunized mice in comparison to pAK3-sod immunized mice (Khera et al., 2005).
Challenge Protocol: No challenge experiment was performed in mice. Guinea pigs were used for protection assay for this vaccine.
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: The levels of IFN-γ in supernatants from pAK3-sod and pAK4-sod immunized mice significantly increased compared to the plasmid vector control groups (Khera et al., 2005).
- Detailed Gene Information: Click here.
Host Gene Response of Il10 (interleukin 10)
- Gene Response: Compared to the plasmid pAK3 and pAK4 controls, expression of the SODA antigen increased IL-10 levels in DNA vaccine pAK3-sod and pAK4-sod immunized mice. The extra CpG motifs in the pAK4 plasmid decreases the IL-10 level in pAK4-sod immunized mice compared to pAK3-sod immunized mice (Khera et al., 2005).
- Detailed Gene Information: Click here.

j. Guinea pig Response

Host Strain: Outbred guinea pigs of the Duncan Hartley strain
Challenge Protocol: Guinea pigs (in groups of six) were immunized intramuscularly with three doses (of 100 μg each) of pAK4, pAK4-E6 expressing ESAT-6, pAK4-αcry expressing α-crystallin, or pAK4-sod at 3 weeks intervals. Four weeks after the last booster, guinea pigs were subcutaneously challenged with 1 × 10^5 CFU of M. tuberculosis H37Rv. Guinea pigs were euthanised 4 weeks after challenge (Khera et al., 2005).
Efficacy: DNA vaccine expressing superoxide dismutase imparted the maximum protection as observed by a 50 and 10 folds reduction in bacillary load in spleens and lungs, respectively, in comparison to immunization with vector control. Among the animals vaccinated with various DNA vaccines, pAK4-sod immunized guinea pigs exhibited the minimal granuloma (Khera et al., 2005).

26. M. tuberculosis DNA vaccine pcDNA3.1+/Ag85A DNA encoding Ag85A

a. Vaccine Ontology ID:

VO_0004356

b. Type:

DNA vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of FbpA (Ag85A)

Type: DNA vaccine construction
Description: Vector pcDNA3.1 expressed Mycobacterium antigen 85A (Ag85A) (Wang et al., 2010).
Detailed Gene Information: Click here.

f. Vector:

pcDNA3.1 (Wang et al., 2010)

g. Immunization Route

Intramuscular injection (i.m.)

h. Mouse Response

Vaccine Immune Response Type: VO_0000286
Immune Response: Orally administrated liposomal-pcDNA3.1+/Ag85A DNA was efficiently incorporated into mucosal epithelium of the small intestine, Peyer's patches (PP). This initiated a Ag85A-specific Th1 dominant immune response, as evidenced by increased secretion of IL-2, IFN-γ, and no change of IL-4 (Wang et al., 2010)
Efficacy: Liposomal-pcDNA3.1+/Ag85A DNA significantly increased the protection by giving a markedly reduction of TB burden in the lung, demonstrating that the TB-specific immune responses elicited by oral administration of liposomal-pcDNA3.1+/Ag85A DNA led to an enhanced host defense against TB infection. (Wang et al., 2010)

27. M. tuberculosis HBHA Protein Vaccine

a. Vaccine Ontology ID:

VO_0004059

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

HBHA

e. Gene Engineering of HBHA

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0001250
Description: DDA-MPL

g. Adjuvant:

VO ID: VO_0001261

h. Immunization Route

subcutaneous injection

i. Mouse Response

Host Strain: C57BL/6
Vaccination Protocol: were vaccinated subcutaneously three times with 5 μg of either HPLC-purified HBHA (nHBHA) or recombinant HBHA emulsified in 150 μg of dimethyldioctadecylammonium bromide (DDA) (Acros Organics, Morris Plains, N.J.)-monophosphoryl lipid A (MPL) adjuvant or with the adjuvant combination alone (Parra et al., 2004).
Challenge Protocol: Mice were challenged with aerosolized M. tuberculosis Erdman (200 CFU) 4 weeks following the final immunization (Parra et al., 2004).
Efficacy: The HBHA-containing vaccine gave a approximately 0.7-log reduction in CFU in both mouse lungs and spleens compared to adjuvant controls 28 days following challenge (Parra et al., 2004).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: There was a ~10-fold increase in the amount of IFN-γ released in the ex vivo assay by spleen cells from HBHA-immunized mice compared to cells from naïve mice or mice immunized with only adjuvant, which was a significantly higher amount. Splenocytes from immunized mice were incubated with murine BMMΦ infected with M. bovis BCG for 72 hours. Lymph node-derived lymphocytes from HBHA-vaccinated mice released approximately eightfold more IFN-γ in the presence of HBHA-stimulated macrophages than cells from naïve or adjuvant control mice released, which was also significant (Parra et al., 2004).
- Detailed Gene Information: Click here.

28. M. tuberculosis Mtb72F Protein Subunit Vaccine

a. Vaccine Ontology ID:

VO_0011412

b. Type:

Subunit vaccine

c. Status:

Research

d. Gene Engineering of Mtb72F

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

e. Adjuvant:

VO ID: VO_0001264
Description: AS02A (Skeiky et al., 2004).

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Host Strain: C57/BL6
Vaccination Protocol: Mice were immunized three times (3 wk apart) with 8 µg of rMtb72F formulated with the adjuvants AS02A. For protein formulations, the required immunization dose of rMtb72F (typically 8 µg) was brought up to either 50 or 43 µl with 1x PBS (pH 6.8) and mixed with 57 µl of AS02A. Mice were injected with a total volume of 100 µl/mouse via the i.m. (tibialis) route with 50 µl/leg. Positive control mice were immunized with BCG (5 x 10^4 CFU) in the base of the tail (once), and negative control animals were injected with saline, adjuvant alone, or DNA vector (Skeiky et al., 2004).
Challenge Protocol: Thirty days after the last immunization, mice were challenged by low dose aerosol exposure with M. tuberculosis H37Rv strain (Skeiky et al., 2004).
Efficacy: Immunization of mice with Mtb72F protein formulated in the adjuvant AS02A resulted in the elicitation of a moderate IFN-gamma response and a weak CD8(+) T cell response to Mtb32c. Mtb72F immunization resulted in the protection of C57BL/6 mice against aerosol challenge with a virulent strain of M. tuberculosis(Skeiky et al., 2004).

29. M. tuberculosis phoP mutant SO2

a. Tradename:

M. tuberculosis phoP mutant strain

b. Vaccine Ontology ID:

VO_0000588

c. Type:

Live attenuated vaccine

d. Gene Engineering of PhoP

Type: Gene mutation
Description: The mutant strain was constructed by a single gene (phoP) disruption of the parental M. tuberculosis MT103 strain. PhoP has shown involvement in the regulation of complex mycobacterial lipids implicated in the virulence of M. tuberculosis (Martin et al., 2006).
Detailed Gene Information: Click here.

e. Preparation

Rabbits were treated with four doses of phoP prior to isolating polyclonal antibodies against phoP. Anti-phoP and monoclonal antibodies against ESAT-6 were obtained. Horseradish peroxidase-labelled goat anti-rabbit antibodies served as secondary antibodies (Martin et al., 2006). Mycobacterial cell-free protein extracts from M. tuberculosis were prepared, filtered, and precipitated with 45% (w/v) ammonium sulphate (Martin et al., 2006).

f. Description

The phoP transcription factor is part of the two-component regulatory signal transduction system component which recently was linked to regulation of complex mycobacterial lipids implicated in the virulence of M. tuberculosis, as well as to multidrug resistance occurrences in a clinical isolate (Martin et al., 2006; Perez et al., 2001) established the selection of phoP as a vaccine candidate by showing that the mutant SO2 strain shows impaired multiplication within cultured macrophages and in vivo infection models (Martin et al., 2006).

g. Mouse Response

Host Strain: Balb/C mice
Vaccination Protocol: Groups of four Balb/C mice were vaccinated with either 2.5x10^3 CFUs of SO2 or 8x10^3 CFUs of M. bovis BCG and later sacrificed at 7, 14, 21, 28, 45, and 60 days post-vaccination (Martin et al., 2006).
Challenge Protocol: To asses protective efficacy of SO2 in Balc/c mice, mice were challenged by intravenous route with 2.5x10^5 CFU M. tuberculosis H37Rv at week 8 and sacrificed four weeks later.
Efficacy: Vaccination with M. tuberculosis SO2 induced a significantly higher proportion of CD4+/IFN-gamma+ producing cells after 45 days of vaccination when compared with BCG (Martin et al., 2006). The proportion of CD8+/IFN-gamma+ producing cells was consistently higher in the M. tuberculosis SO2group. SO2 strain and BCG both conferred significant levels of protection compared to saline controls, with reductions of 1.5 and 1.3 log10 reductions in cfu counts in lung and spleen (Martin et al., 2006). However,
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: Vaccination with M. tuberculosis SO2 induced a significantly higher proportion of CD4+/IFN-γ+ producing cells in mice 45 days after vaccination when compared with BCG (Martin et al., 2006).
- Detailed Gene Information: Click here.

h. Guinea pig Response

Vaccination Protocol: Groups of six guinea pigs were used in two procedures. Guinea pigs were vaccinated subcutaneously with 250μl of 5x10^4 CFU BCG (Pasteur) or SO2 in both the low-dose and high-dose challenge experiments. Saline was also used in the low-dose experiments.
Side Effects: SO2-treated guinea pigs gained weight. No visible or clinical signs of disease after challenge.
Challenge Protocol: In the low dose challenge, guinea pigs were aerosol challenged at 12 weeks using a Henderson apparatus and 2x10^6 CFU/ml M. tuberculosis water suspension to give estimated inhaled dose of 10-50 CFU/lung. In high dose challenge, guinea pigs were aerosol challenged at 10 weeks with 5x10^7 CFU/ml M.tuberculosis in water suspension to give approimately 500 CFUs to the lungs. Animals were killed by peritoneal overdose of sodium pentabarbitone (Chandra et al., 2006).
Efficacy: A significant difference was observed between non-vaccinated and vaccinated (both BCG and SO2-recipient) groups receiving low-dose treatment, whereas no significant difference was evident between the BCG and SO2 groups. In high dose challenge, all non-vaccinated and BCG-vaccinated were euthanized according to set humane end-point, while all SO2-vaccinated groups showed significantly longer survival than BCG groups and saline-treated groups. Pulmonary disease most evident in non-vaccinated guinea pigs. Consolidation was significantly less in the SO2-vaccinated groups versus non-vaccinated and BCG-vaccinated groups. "M. tuberculosis SO2 strain was superior to BCG in conferring enhanced survival to infected guinea pigs, reduction in the severity of the disease in the lung, and dissemination of infection to the spleen" (Martin et al., 2006).

i. Mouse Response

Host Strain: CB-17/lcr lco SCID specific pathogen free (spf)
Vaccination Protocol: No vaccination was employed here. Purpose of experiment is to assess survival of SCID mice following infection with either SO2 or BCG (Martin et al., 2006).
Persistence: All SCID mice infected with SO2 strain survived for >245 days, whereas those infected with M. tuberculosis MT103 and SO2-pSO5 died within 62 days of infection(Martin et al., 2006).
Challenge Protocol: Ten mice per experimental group were infected with 200μl PBS containing 2x10^5, 2x10^4, or 2x10^3 viable BCG Pasteur, or 5.4x10^6, 5.4x10^5, or 5.4x10^4 viable SO2. Aerosol infection was also used to nebulize with 7 ml of M. tuberculosis suspension, providing 20 viable bacilli within the lungs. Survival times were determined using Mantel-Haenszel test (Martin et al., 2006).

30. M. tuberculosis secA2 mutant

a. Vaccine Ontology ID:

VO_0000564

b. Type:

Live, attenuated vaccine

c. Gene Engineering of SecA2

Type: secA2 transformation into M. tuberculosis
Description: The secA2 gene was amplified by PCR from virulent H37Rv genomic DNA, cloned into a pGEM vector, and later excised as a fragment and cloned into pMV361 (Hinchey et al., 2007).
Detailed Gene Information: Click here.

d. Preparation

Three virulent strains (M. tuberculosis H37Rv, HN878, and ΔsecA2 mutants) were obtained and grown on Middlebrook 7H9 broth or 7H10 agar containing 10% (vol/vol) oleic acid-albumin-dextrose-catalase, 0.5% glycerol, and 0.05% (vol/vol) Tween-80. Recombinant strains were selected using ampicillin, hygromycin, and kanamycin.

e. Description

The secA2 gene encodes a component of a virulence-associated bacterial protein secretion system and is required for prevention of apoptosis in macrophages infected with M. tuberculosis. The apoptotic response is related to secA2-dependent secretion of mycobacterial superoxide dismutase A (SodA). M. tuberculosis H37Rv with deleted secA2 induces apoptosis, as indicated by TUNEL and polycaspase activation in mouse and human macrophages (Hinchey et al., 2007). Extrinsic and intrinsic caspase pathways were linked to initiation of apoptosis in the secA2-deleted mutants via caspase 8 and 9 activity. The secretion of SodA appears to be important for the inhibition of apoptosis by secA2-dependent mechanisms. Furthermore,

f. Mouse Response

Host Strain: B6.PL (Thy1.1+), SIINFEKL/H-2K^b-reactive TCR-transgenic OT-1
Vaccination Protocol: Donor splenocytes from Rag1 deficient OT1 TCR-transgenic mice were isolated and labeled with 20 μg CFSE and washed. Aliquots containing either 3x10^5 or 1x10^7 labeled cells were injected into lateral tail vein prior to subcutaneous or i.v. infection with 1x10^8 CFU M. tuberculosis ΔsecA2-OVA or control bacteria strains. Splenocytes were harvested and stained 5-7 days post-infection and analyzed by flow cytometry to determine percentage of undivided (high-CFSE) cells in the Thy1.2+ population (Hinchey et al., 2007).
Immune Response: OT-1 T cells infected with H37Rv, H37Rv-OVA, and ΔsecA2-OVA showed negligible, modest, and massive increases in proliferation of transferred T cells. SecA2 addition can restore OT-1 T cell priming activities in the mutants. Furthermore, SIINFEKL epitope is produced in all mutants in an immunogenic form. Additional evidence suggested that tight linkage exists among CD8+ T cell priming, loss of SodA secretion, and blocked apoptosis (Hinchey et al., 2007).
Challenge Protocol: No challenge was implemented here.
Description: Procedure was designed to visualize and better understand the priming of MHC class I restricted CD8+ T cells with SIINFEKL fusion protein, as well as understand the relationship between SodA secretion, apoptosis, and secA2 (Hinchey et al., 2007).

g. Mouse Response

Host Strain: C57BL/6
Vaccination Protocol: Mice received 5x10^5 OT-1 cells one day before infecting subcutaneously with either 1x10^6 CFU H37Rv-OVA or ΔsecA2-OVA. Animals were sacrificed at 2, 4, or 20 weeks prior to splenic isolation and antibody labeling. Lympocyte subpopulations (CD44high,CD62high; CD44highCD62low) were quantified and separated using flow cytometry (Hinchey et al., 2007).
Immune Response: Infection with H37Rv, H37Rv-OVA or ΔRD1-OVA induced background levels of IFN-gamma spot-forming cells while the ΔsecA2-OVA infection induces a 10-fold increase of similar spots above the background levels. Additional evidence from an in vivo CTL assay for SIINFEKL -induced CD8+ T cell activity strengthened the claim that enhanced T cell priming in the sec2A deletion mutants is restricted to CD8+ T cell compartment (Hinchey et al., 2007).
Challenge Protocol: No challenge was introduced in this protocol.
Description: Purpose of this experiment was to analyze memory T cell populations and abundance within the native splenic environment, and to determine the extent of antigen transmission to dendritic cells and enhanced CD8+ T cell priming (Hinchey et al., 2007).

h. Mouse Response

Host Strain: C57BL/6
Vaccination Protocol: Mice were vaccinated subcutaneously with 1x10^6 CFUs of either M. tuberculosis ΔsecA2 or M. bovis BCG-Pasteur.
Persistence: Substantial growth of HN878 observed in lungs and spleens within one month of challenge (Hinchey et al., 2007), whereas both M. tuberculosis ΔsecA2 or M. bovis BCG-Pasteur showed decreased loads at the same interval.
Immune Response: Mild inflammation including small/compact granulomas containing lymphocytes were observed for animals vaccinated with BCG or ΔsecA2 (Hinchey et al., 2007).
Challenge Protocol: Mice were challenged via aerosol route two months post-vaccination using GlasCol inhalation chamber. Approximately 50-100 CFUs of either M. tuberculosis Beijing/W (HN878) or Erdman strains were administered using this approach. Mice were sacrificed at 1, 3, and 5 months post-challenge prior to isolation of lung and spleen. Mice infected with the Erdman strain were observed daily until euthanization in a second survival study (Hinchey et al., 2007).
Efficacy: Protection by ΔsecA2 was significantly greater than in animals infected with M. bovis BCG-Pasteur at one month post-challenge (Hinchey et al., 2007). Furthermore, the protection offered by ΔsecA2 was still significant after five months post-challenge despite non-significant BCG protection at the same interval.

i. Mouse Response

Host Strain: Outbred Hartley guinea pigs
Vaccination Protocol: Guinea pigs were vaccinated intradermally with 1x10^3 M. tuberculosis ΔsecA2 or BCG-Pasteur (Hinchey et al., 2007).
Persistence: Lower CFUs observed in ΔsecA2-immunized animals versus BCG and control animals (Hinchey et al., 2007).
Immune Response: Minor fibrosis observed for in ΔsecA2-immunized animals at 2 months post-challenge (Hinchey et al., 2007).
Challenge Protocol: Animals were challenged six weeks post-vaccination via aerosol route with 10-30CFUs of M. tuberculosis H37Rv strains (Madison chamber). Spleen, lung, and lymph node tissues were isolated at either 1 or 2 months post-challenge. (Hinchey et al., 2007).
Efficacy: The ΔsecA2-immunized animals exhibited significant decreases (p<.01) in lung, splenic, and mediastinal lymph node CFUs versus control animals. Notable differences in CFU counts and organ size in the mediastinal lymph nodes were observed in only the M. tuberculosis ΔsecA2-innoculated animals, including only minor fibrosis at two months post-challenge (Hinchey et al., 2007).
Description: Guinea pigs were vaccinated and challenged for CFU determination and histopathology comparison between different attenuated strains of M. tuberculosis and M. bovis. The results from the guinea pig study suggest that the . M. tuberculosis ΔsecA2 exceeds the efficacy of BCG in several aspects and may be a viable vaccine candidate (Hinchey et al., 2007).

31. M.S-Δesx-3-IKEPLUS (M. tuberculosis)

a. Vaccine Ontology ID:

VO_0004689

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Preparation

(Sweeney et al., 2011)

f. Immunization Route

Intramuscular injection (i.m.)

32. Mtb72f fusion protein

a. Vaccine Ontology ID:

VO_0004108

b. Type:

Fusion protein with adjuvant

c. Gene Engineering of PepA

Type: Fusion protein from co-expressed ORFs
Description: Two open reading frames for M. tuberculosis antigenic proteins Mtb32 and Mtb39 (Rv0125 and Rv1196, respectively) were combined and expressed as a single recombinant polyprotein of size 72kDa. Each ORF was PCR amplified and subcloned into plasmids prior to transformation into E. coli. Correct inserts and orientation were identified by restriction digests and DNA sequencing. The final Mtb72f fusion protein acts as an activator of IFN-gamma in CD4 and CD8 T cells (Skeiky et al., 2004).
Detailed Gene Information: Click here.

d. Gene Engineering of PPE18

Type: Fusion protein from co-expressed ORFs
Description: Two open reading frames for M. tuberculosis antigenic proteins Mtb32 and Mtb39 (Rv0125 and Rv1196, respectively) were combined and expressed as a single recombinant polyprotein of size 72kDa. Each ORF was PCR amplified and subcloned into plasmids prior to transformation into E. coli. Correct inserts and orientation were identified by restriction digests and DNA sequencing. The final Mtb72f fusion protein acts as an activator of IFN-gamma in CD4 and CD8 T cells (Skeiky et al., 2004).
Detailed Gene Information: Click here.

e. Adjuvant:

VO ID: VO_0001264
Description: Adjuvants included AS02A or AS01B (Skeiky et al., 2004).

f. Preparation

See genetic engineering section for details regarding construction of rMtb72F. Vaccines included rMtb72F combined with either of two adjuvants, or Mtb72F alone (Skeiky et al., 2004). Immunization dose was either 50 or 43 μl with 1x PBS (pH 6.8) mixed with 50 μl of AS01B or 57 μl of AS02A, respectively (Skeiky et al., 2004).

g. Description

Mice were immunized three times (at 3 week intervals) with 8μg of recombinant Mtb72F (rMtb72F) (Skeiky et al., 2004).

h. Mouse Response

Host Strain: C57/BL6 female mice
Vaccination Protocol: Mice were age-matched (4-6 weeks) per experiment, and immunized 3 times (2 wk apart) with 8μg of rMtb72f and selected adjuvants. Immunization dose was increased from 8μg using 50 or 43 μl of PBS with 50 or 57 μl of AS01B or AS02A adjuvants. In total, 100 μl of vaccine was injected via i.m. (tibialis) route with 50 μl/leg (Skeiky et al., 2004). Additional culturing protocols were included for mononuclear and splenic cells (see (Skeiky et al., 2004)). Mice immunized with 5x10^4BCG in base of tail served as positive controls. Negative controls were injected with saline, adjuvant alone, or DNA vector.
Persistence: The extent of protection with Mtb72F represents the longest documented survival end point reported for any defined subunit vaccine (~40% survival after 80 weeks post-challenge) (Skeiky et al., 2004).
Challenge Protocol: Thirty days after the last immunization, mice were challenged by low-dose aerosol exposure (50-100 bacteria in lungs) with M. tuberculosis H37Rv strain. Mice were euthanized four weeks later prior to lung and spleen homogenate preparation.
Efficacy: Mice immunized with DNA developed an Mtb72f Ab response of the IgG2a but not IgG1 subclasses which was directed against N-terminal (Mtb32c) molecules. Strong IFN-gamma responses were elicited in response to rMtb72F, rMtb32c, Mtb39, and PPD treatments. Mice immunized with rMtb72f and either adjuvant mounted strong IgG1 and IgG2a responses against Mtb72F. The AS01B-Mtb72F formulation elicited robust CD8+ T cell responses (directed against Mtb32c), whereas the AS02A-Mtb72F formulation were weaker than the AS01B formulation. However, both adjuvants when combined with Mtb72f could elicit ~0.6 log reduction in bacteria burden of mouse lungs. The Mtb72F-DNA provided 0.7-1.0 log reduction in bacterial load, which is comparable to BCG results (Skeiky et al., 2004).

i. Guinea pig Response

Vaccination Protocol: Similar to mouse protocol, the guinea pigs were immunized three times (3 weeks apart) with 200μg Mtb72F-DNA with 250μl of 1xPBS @ pH 7.0, 20-μg dose of rMtb72f, or a mixture of these components in a total 250uL dose containing AS02A. Guinea pigs were immunized with 125μl of final formula in the leg (Skeiky et al., 2004). BCG administered to some animals via intradermal route. ***
Persistence: Evidence suggests that the protection can last for > 1 year (Skeiky et al., 2004).
Challenge Protocol: Animals were challenged with virulent H37Rv strain via aerosol route after 13 weeks, with nebulizer providing 20-50 bacteria into the lungs (Skeiky et al., 2004).
Efficacy: After 30 wk postchallenge, four of five guinea pigs immunized with rMtb72F and three of five immunized with Mtb72F-DNA were alive, whereas after 70 weeks, two of five in DNA-immunized groups were still alive.

33. MVA/IL-15/5Mtb vaccine

a. Vaccine Ontology ID:

VO_0004263

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Antigen

A recombinant modified vaccinia Ankara (MVA) construct (MVA/IL-15/5Mtb) which overexpresses five Mycobacterium tuberculosis antigens (antigen 85A, antigen 85B, ESAT6, HSP60, and Mtb39) (Kolibab et al., 2010).

e. Gene Engineering of FbpA (Ag85A)

Type: Recombinant vector construction
Description:
Detailed Gene Information: Click here.

f. Gene Engineering of Ag85B from M. tuberculosis H37Rv

Type: Recombinant vector construction
Description:
Detailed Gene Information: Click here.

g. Gene Engineering of EsxA (ESAT-6)

Type: Recombinant vector construction
Description:
Detailed Gene Information: Click here.

h. Adjuvant:

VO ID: VO_0001342

i. Immunization Route

subcutaneous injection

j. Mouse Response

Host Strain: C57BL/6
Vaccination Protocol: C57BL/6 female mice were vaccinated once subcutaneously with 10^6 CFU of BCG Pasteur 6 weeks before challenge. For the wild-type MVA vector (as a control) or the MVA/IL-15/5Mtb vaccine, mice were administered two doses of 5 × 10^7 PFU subcutaneously at 1 month apart (Kolibab et al., 2010).
Challenge Protocol: Five mice from each group were aerogenically challenged with M. tuberculosis Erdman K1 (Trudeau Mycobacterial Culture Collection) suspended in phosphate-buffered saline (PBS) at a concentration known to deliver 200 CFU in the lungs over a 30-min exposure in a Middlebrook chamber (Kolibab et al., 2010).
Efficacy: Mice vaccinated with the MVA/IL-15/5Mtb construct had significant protection at 2 and 12 months post challenge. At 12 months postvaccination, highly persistent protective responses were seen in the lungs of mice immunized with BCG vaccine or our MVA/IL-15/5Mtb construct (Kolibab et al., 2010).
Host Gene Response of Cxcl10
- Gene Response: Two and sixteen months post vaccination with MVA/IL-15/5Mtb, Cxcl10 was significantly upregulated in mice lungs as compared to naive control mice. However, it was significantly down-regulated as compared to mice vaccinated with BCG 16 months post vaccination (Kolibab et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of Cxcl11
- Gene Response: Two months post vaccination with MVA/IL-15/5Mtb, Cxcl11 was significantly upregulated in mice lungs as compared to naive control mice. However, it was significantly down-regulated as compared to mice vaccinated with BCG 16 months post vaccination (Kolibab et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of Cxcl9
- Gene Response: Two and sixteen months post vaccination with MVA/IL-15/5Mtb, Cxcl9 was significantly upregulated in mice lungs as compared to naive control mice. However, it was significantly down-regulated as compared to mice vaccinated with BCG 16 months post vaccination (Kolibab et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of Ebi3
- Gene Response: Two months post vaccination with MVA/IL-15/5Mtb, IL-27 beta (Ebi3) was significantly upregulated in mice lungs as compared to naive control mice
- Detailed Gene Information: Click here.
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: Two and sixteen months post vaccination with MVA/IL-15/5Mtb, IFN-gamma was significantly upregulated in mice as compared to naive control mice in lungs. However, it was significantly down-regulated as compared to mice vaccinated with BCG (Kolibab et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of Il12b
- Gene Response: Two months post vaccination with MVA/IL-15/5Mtb, IL12b (IL12 p40 subunit) was significantly upregulated in mice lungs as compared to naive control mice (Kolibab et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of Il15
- Gene Response: IL-15 was used as an adjuvant for the modified vaccinia Ankara vaccine (MVA). Homologous prime/boost studies showed that the MVA/IL-15/5Mtb vaccine induced moderate but highly persistent protective immune responses for at least 16 months after the initial vaccination and that the interval between the prime and boost did not significantly alter vaccine-induced antituberculosis protective immunity. Organ (lung or spleen) bacterial burdens (CFU) in the MVA/IL-15/5Mtb vaccine-immunized mice were greater than those in BCG vaccinated animals, but less than those in mice vaccinated with MVA alone. MVA/IL-15/5Mtb also induced protection that was equivalent to BCG, whereas MVA alone did not induce protective immunity (Kolibab et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of Il17f
- Gene Response: Two and sixteen months post vaccination with MVA/IL-15/5Mtb, IL-17F was significantly upregulated in mice lungs as compared to naive control mice. However, it was significantly down-regulated as compared to mice vaccinated with BCG (Kolibab et al., 2010).
- Detailed Gene Information: Click here.

34. MVA85A prime and BCG boost

a. Vaccine Ontology ID:

VO_0004106

b. Type:

Subunit vaccine

c. Antigen

Mycobacterial mycolyl transferase Ag85A (Vordermeier et al., 2004).

d. Gene Engineering of FbpA (Ag85A)

Type: Recombinant virus expressing Ag85A
Description:
Detailed Gene Information: Click here.

e. Virulence

MVA is avirulent in normal and immunocompromised animals and has shown no significant side-effects in 120,000 vaccinated humans. Recombinant fowlpox FP9 cannot replicate in mammalian cells and is not pathogenic in mammals or birds (Vordermeier et al., 2004).

f. Cattle Response

Host Strain: Friesians, 6-mo. old
Vaccination Protocol: Groups of four calves were subjected to one of five schedules in the first experiment: Group 1: MVA85A was administered at week 0, followed by BCG at week 9. Group 2: BCG Pasteur was administered at week 0, followed by MVA85A at week 9. Group 3: BCG administered at week 0, FP85A (fowlpox) administered at week 8, followed by MVA85A at week 10. Group 4: FP85A administered at weeks 0 and 3, followed by MVA85A at week 8. Group 5: (treatments reversed versus group 4): MVA85A administered at weeks 0 and 3, followed by FP85A at week 8 (Vordermeier et al., 2004). A 10^6 CFU dose of BCG was injected subcutaneously in side of neck with 1 ml PBS. MVA8A and FP85A doses were each administered as 10^9 plaque-forming units (PFUs) in PBS in a total 0.1 mL injection volume at each injection site. Animals skin tested with SICCT 14 weeks post-first vaccination (Vordermeier et al., 2004). In the second experiment, six calves were vaccinated s.c. with 10^6 CFU BCG Pasteur in 1 ml PBS. Three calves each were revaccinated with either a 10^9 PFU dose of MVA85 (intradermal) or 10^6 CFU dose of BCG Pasteur, 5 weeks later (Vordermeier et al., 2004).
Immune Response: Prime-boost protocols, using recombinant MVA and BCG in combination (groups 1-3), resulted in significantly higher frequencies of Ag85-specific IFN-gamma-secreting cells than the two viral vectors used in combination (P=0.0055), or BCG used alone (groups 2 and 3, P=0.04) (Vordermeier et al., 2004).

35. Mycobacterium tuberculosis DeltasigF mutant vaccine

a. Type:

Live, attenuated vaccine

b. Status:

Research

c. Host Species for Licensed Use:

None

d. Host Species as Laboratory Animal Model:

Rabbit

e. Gene Engineering of sigF

Type: Gene mutation
Description:
Detailed Gene Information: Click here.

f. Immunization Route

Intradermal injection (i.d.)

36. Mycobacterium tuberculosis drrC mutant vaccine

a. Vaccine Ontology ID:

VO_0002780

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of drrC

Type: Gene mutation
Description: This drrC mutant is from Mycobacterium tuberculosis (Pinto et al., 2004).
Detailed Gene Information: Click here.

f. Immunization Route

Intravenous injection (i.v.)

g. Mouse Response

Persistence: A drrC mutant is highly attenuated in mice (Pinto et al., 2004).
Efficacy: A drrC mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Pinto et al., 2004).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: The drrC− strain was able to induce splenic anti–M. tuberculosis IFN-γ–secreting T cells responsive to CFP in mice to a level equal to that induced by M. tuberculosis infection and much greater than the amount induced by BCG and control mice. The drrC− mutant was also able to generate high numbers of IFN-γ–secreting cells that recognize the M. tuberculosis ESAT-6 antigen, which BCG and control mice could not do. IFN-gamma levels in both cases were comparable to levels induced by the wild type. This effect began 4 weeks after infection and was sustained long-term (24 weeks after infection) (Pinto et al., 2004).
- Detailed Gene Information: Click here.

37. Mycobacterium tuberculosis fadD26 mutant vaccine

a. Vaccine Ontology ID:

VO_0002781

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of fadD26

Type: Gene mutation
Description: This fadD26 mutant is from Mycobacterium tuberculosis (Infante et al., 2005).
Detailed Gene Information: Click here.

f. Immunization Route

Intratracheal immunization

g. Mouse Response

Persistence: A fadD26 mutant is attenuated in mice (Infante et al., 2005).
Efficacy: A fadD26 mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Infante et al., 2005).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: The fadD26 mutant induced a progressive increase in IFN-γ expression in lung homogenates of mice, peaking at day 60, and 2 months later it had decreased by only 20% from its maximal level. However, it induced significantly lower expression than that induced by the wild type pathogen (MT-103) from days 1-21 post infection (Infante et al., 2005).
- Detailed Gene Information: Click here.
Host Gene Response of Il4 (interleukin 4)
- Gene Response: A fadD26 mutant induced a significantly lower and stable IL-4 expression as compared to the wild type (MT103) during the first month of infection in mice, followed by an increase of 50% at days 60 and 120. IL-4 levels of a fadD26 mutant-vaccinated mice were significantly lower than those infected with the wild type on days 3, 7, 21, 28, and 60 post infection in lung homogenates (Infante et al., 2005).
- Detailed Gene Information: Click here.
Host Gene Response of Nos2
- Gene Response: Mice infected with the fadD26 mutant showed significant higher iNOS expression than the parent strain in lung homogenates days 21, 28 and 60 post infection. Results showed that the fadD26 mutant is also more efficient than its parental strain at inducing iNOS expression (Infante et al., 2005).
- Detailed Gene Information: Click here.
Host Gene Response of TNF-alpha
- Gene Response: The fadD26 mutant induced stable TNF-α expression in mice, significantly lower than that induced by MT-103 (WT strain) after 1 week of infection. In lung homogenates, fadD26 mutant vaccination induced significantly lower TNF-alpha levels on days 7, 14, 28, and 60 as compared to mice vaccinated with the wild type (Infante et al., 2005).
- Detailed Gene Information: Click here.

38. Mycobacterium tuberculosis leuD mutant vaccine

a. Vaccine Ontology ID:

VO_0002782

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of leuD

Type: Gene mutation
Description: This leuD mutant is from Mycobacterium tuberculosis (Hondalus et al., 2000).
Detailed Gene Information: Click here.

f. Immunization Route

Intravenous injection (i.v.)

g. Mouse Response

Persistence: A leuD mutant is attenuated in mice (Hondalus et al., 2000).
Efficacy: A leuD mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Hondalus et al., 2000).

39. Mycobacterium tuberculosis lysA/secA2 mutant vaccine

a. Vaccine Ontology ID:

VO_0002783

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of lysA

Type: Gene mutation
Description: This lysA/secA2 mutant is from Mycobacterium tuberculosis (Hinchey et al., 2011).
Detailed Gene Information: Click here.

f. Gene Engineering of SecA2

Type: Gene mutation
Description: This lysA/secA2 mutant is from Mycobacterium tuberculosis (Hinchey et al., 2011).
Detailed Gene Information: Click here.

g. Immunization Route

subcutaneous injection

h. Mouse Response

Persistence: A lysA/secA2 mutant is highly attenuated in mice (Hinchey et al., 2011).
Efficacy: A lysA/secA2 mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Hinchey et al., 2011).

40. Mycobacterium tuberculosis mce-2 mutant vaccine

a. Vaccine Ontology ID:

VO_0004279

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of mce-2

Type: Gene mutation
Description: This mce-2 mutant is from Mycobacterium tuberculosis (Aguilar et al., 2006).
Detailed Gene Information: Click here.

f. Gene Engineering of mce-3

Type: Gene mutation
Description: This mce-2/mce-3 mutant is from Mycobacterium tuberculosis (Aguilar et al., 2006).
Detailed Gene Information: Click here.

g. Immunization Route

subcutaneous injection

h. Mouse Response

Persistence: A mce-2 mutant is attenuated in mice (Aguilar et al., 2006).
Efficacy: A mce-2 mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Aguilar et al., 2006).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: Mutant mce-2 induced a lower but progressive expression of IFN-γ than the parent strain in mice, peaking at day 120 post-infection. The IFN-gamma response in lungs was significantly lower in mice immunized with mce-2 mutants on days 1, 3, 7, 14, and 21 days post infection as compared to mice infected with the wild type (Aguilar et al., 2006).
- Detailed Gene Information: Click here.
Host Gene Response of Il4 (interleukin 4)
- Gene Response: The mce-2 mutant maintained low IL-4 expression until day 60 post-infection, showing slight increase at day 120. The expression of IL-4 was higher in the lungs of mice infected with the parental strain. Infection with the wild type strain induced significantly more IL-4 than that induced by immunization with the mutant on days 21, 28, and 60 post infection (Aguilar et al., 2006).
- Detailed Gene Information: Click here.

41. Mycobacterium tuberculosis mce-3 mutant vaccine

a. Vaccine Ontology ID:

VO_0004288

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Mouse

e. Gene Engineering of mce-3

Type: Gene mutation
Description: This mce-3 mutant is from Mycobacterium tuberculosis (Aguilar et al., 2006).
Detailed Gene Information: Click here.

f. Immunization Route

subcutaneous injection

g. Mouse Response

Persistence: A mce-3 mutant is attenuated in mice (Aguilar et al., 2006).
Efficacy: A mce-3 mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Aguilar et al., 2006).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: The mce-3 mutant induced a lower but progressive expression of IFN-γ than the parent strain in mice, peaking at day 120 post-infection. The IFN-gamma response in lungs was significantly lower in mice immunized with mce-3 mutants on days 1, 3, 7, 14, and 21 days post infection as compared to mice infected with the wild type (Aguilar et al., 2006).
- Detailed Gene Information: Click here.
Host Gene Response of Il4 (interleukin 4)
- Gene Response: The mce-3 mutant maintained low IL-4 expression until day 60 post-infection, showing slight increase at day 120. The expression of IL-4 was higher in the lungs of mice infected with the parental strain. Infection with the wild type strain induced significantly more IL-4 than that induced by immunization with the mutant on days 21, 28, and 60 post infection (Aguilar et al., 2006).
- Detailed Gene Information: Click here.

42. Mycobacterium tuberculosis panCD mutant vaccine

a. Vaccine Ontology ID:

VO_0002786

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of panC

Type: Gene mutation
Description: This panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2002).
Detailed Gene Information: Click here.

f. Gene Engineering of panD

Type: Gene mutation
Description: This panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2002).
Detailed Gene Information: Click here.

g. Immunization Route

subcutaneous injection

h. Mouse Response

Persistence: A panCD mutant is attenuated in mice (Sambandamurthy et al., 2002).
Efficacy: A panCD mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Sambandamurthy et al., 2002).

43. Mycobacterium tuberculosis proC mutant vaccine

a. Vaccine Ontology ID:

VO_0002791

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of proC

Type: Gene mutation
Description: This proC mutant is from Mycobacterium tuberculosis (Smith et al., 2001).
Detailed Gene Information: Click here.

f. Immunization Route

Intravenous injection (i.v.)

g. Mouse Response

Persistence: A proC mutant is attenuated in mice (Smith et al., 2001).
Efficacy: A proC mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Smith et al., 2001).

44. Mycobacterium tuberculosis RD1/panCD mutant vaccine

a. Vaccine Ontology ID:

VO_0002787

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of RD1

Type: Gene mutation
Description: This RD1/panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2006).
Detailed Gene Information: Click here.

f. Gene Engineering of panC

Type: Gene mutation
Description: This RD1/panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2006).
Detailed Gene Information: Click here.

g. Gene Engineering of panD

Type: Gene mutation
Description: This RD1/panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2006).
Detailed Gene Information: Click here.

h. Immunization Route

subcutaneous injection

i. Mouse Response

Persistence: An RD1/panCD mutant is attenuated in mice (Sambandamurthy et al., 2006).
Efficacy: An RD1/panCD mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Sambandamurthy et al., 2006).
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: At the 10-day time point, substantial increases (relative to naive mice) were detected in IFN-γ positive CD4 and CD8 lung cells taken from the vaccinated mice (Sambandamurthy et al., 2006).
- Detailed Gene Information: Click here.

45. Mycobacterium tuberculosis sigE mutant vaccine

a. Vaccine Ontology ID:

VO_0002795

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of sigE

Type: Gene mutation
Description: This sigE mutant is from Mycobacterium tuberculosis (Hernandez et al., 2010).
Detailed Gene Information: Click here.

f. Immunization Route

Intratracheal immunization

g. Mouse Response

Persistence: A sigE mutant is attenuated in mice (Hernandez et al., 2010).
Efficacy: A sigE mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Hernandez et al., 2010).
Host Gene Response of Defb3
- Gene Response: A sigE mutant showed a significantly higher and constant expression of the gene encoding β-defensin 3 in the lungs beginning the 7th day post vaccination until the 28th day as compared to mice infected with the parental strain (Hernandez et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of Ifng (Interferon gamma)
- Gene Response: A sigE mutant showed a significantly higher and constant expression of the gene encoding IFN-γ as compared to mice infected with the parental strain. IFN-gamma was significantly upregulated in mice lungs after vaccination with the mutant as compared to infection with the wild type beginning day 1 post-infection and continuing throughout the duration of the experiment (Hernandez et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of Il10 (interleukin 10)
- Gene Response: The expression of IL-10 was higher in sigE mutant-infected mice lungs as compared to infection with the parental strain, but it was significant only 21 and 28 days after the infection (Hernandez et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of Il4 (interleukin 4)
- Gene Response: IL-4 was expressed at a significantly lower level in the sigE mutant-infected mice as compared to mice infected with the wild type pathogen. Significant down regulation of IL-4 by the mutant in mouse lungs was seen days 1-28 post infection as compared to infection with the wild type (Hernandez et al., 2010).
- Detailed Gene Information: Click here.
Host Gene Response of TNF-alpha
- Gene Response: A sigE mutant showed a significantly higher and constant expression of the gene encoding TNF-alpha as compared to mice infected with the parental strain. Vaccination with the mutant significantly up-regulated TNF-alpha in mice lungs on days 1, 21, and 28 post-infection as compared to infection with the wild type (Hernandez et al., 2010).
- Detailed Gene Information: Click here.

h. Human Response

Description: The human monocytic cell line THP-1 was obtained from the ATCC, and cultures were grown (FontÃ¡n et al., 2008).
Host Gene Response of APOBEC3B
- Gene Response: A sigE mutant upregulates APOBEC3B in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (FontÃ¡n et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of C19orf66
- Gene Response: A sigE mutant upregulates C19orf66 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (FontÃ¡n et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of CD70
- Gene Response: A sigE mutant upregulates CD70 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (FontÃ¡n et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of GPR182
- Gene Response: A sigE mutant upregulates GPR182 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (FontÃ¡n et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of HIST1H2BE
- Gene Response: A sigE mutant upregulates HIST1H2BE in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (FontÃ¡n et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of HIST2H4A
- Gene Response: A sigE mutant upregulates HIST2H4A in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (FontÃ¡n et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of PCDHB1
- Gene Response: A sigE mutant upregulates PCDHB1 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (FontÃ¡n et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of PTCD1
- Gene Response: A sigE mutant upregulates PTCD1 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (FontÃ¡n et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of STAG3
- Gene Response: A sigE mutant upregulates STAG3 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (FontÃ¡n et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of TBX21
- Gene Response: A sigE mutant upregulates TBX21 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (FontÃ¡n et al., 2008).
- Detailed Gene Information: Click here.
Host Gene Response of TSC22D4
- Gene Response: A sigE mutant upregulates TSC22D4 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (FontÃ¡n et al., 2008).
- Detailed Gene Information: Click here.

46. Mycobacterium tuberculosis trpD mutant vaccine

a. Vaccine Ontology ID:

VO_0002796

b. Type:

Live, attenuated vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of trpD

Type: Gene mutation
Description: This trpD mutant is from Mycobacterium tuberculosis (Smith et al., 2001).
Detailed Gene Information: Click here.

f. Immunization Route

Intravenous injection (i.v.)

g. Mouse Response

Persistence: A trpD mutant is attenuated in mice (Smith et al., 2001).
Efficacy: A trpD mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Smith et al., 2001).

47. Mycobax

a. Tradename:

Mycobax

b. Manufacturer:

Sanofi Pasteur, Ltd.

c. Vaccine Ontology ID:

VO_0000078

d. CDC CVX code:

e. Type:

Live, attenuated vaccine

f. Status:

Licensed

g. Location Licensed:

USA

h. Host Species for Licensed Use:

Human

i. Antigen

Mycobax is a formulation of live attenuated strain of Mycobacterium bovis (Bacillus Calmette Guerin; BCG).

48. rBCG-Ag85A/ Ag85B

a. Vaccine Ontology ID:

VO_0004757

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species for Licensed Use:

Baboon

e. Gene Engineering of FbpA (Ag85A)

Type: Recombinant vector construction
Description: rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis were constructed (Wang et al., 2012).
Detailed Gene Information: Click here.

f. Gene Engineering of Ag85B from M. tuberculosis H37Rv

Type: Recombinant protein preparation
Description: rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis were constructed (Wang et al., 2012).
Detailed Gene Information: Click here.

g. Preparation

Three rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis (Wang et al., 2012).

h. Immunization Route

Intramuscular injection (i.m.)

i. Mouse Response

Vaccination Protocol: Mice in each group were immunized s.c. once at the base of the tail with 1 × 106 CFU of either rBCG::85A, rBCG::85B, or rBCG::AB in a final volume of 100 μL of PBS (Wang et al., 2012).
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: mice were challenged with virulent M. tuberculosis strain 6 weeks after immunization (Wang et al., 2012).
Efficacy: rBCG::AB strain could provide the strongest short-term and long-term protection in the lung against intravenous infection with virulent M. tuberculosis than rBCG::261 control and other two rBCG strains overexpressing single antigen. The stronger and longer-lasting protection provided by rBCG::AB than rBCG::261 was correlated with systemic in vitro antigen-specific IFN-γ responses (Wang et al., 2012).

49. rBCG-Ag85A[Tokyo]

a. Vaccine Ontology ID:

VO_0000565

b. Type:

Live, attenuated vaccine

c. Antigen

BCG and Mycobacterium antigen Ag85A (Sugawara et al., 2007).

d. Gene Engineering of FbpA (Ag85A)

Type: Recombinant vector construction
Description: The Ag85A gene was amplified by PCR and subcloned into the pCR4 vector. The gene was inserted into the pBBN vector (Ag85A-HA) possessing a hemagglutinin (HA) tag at its 5′ end. The Ag85A-HA was expressed in Escherichia coli. The Ag85A-HA gene was then introduced into the downstream region of the pHPS integration vector. The vector was then electroporated into BCG Tokyo. The resulting recombinant was named (rBCG-Ag85A[Tokyo]) (Sugawara et al., 2007).
Detailed Gene Information: Click here.

e. Guinea pig Response

Host Strain: Hartley female guinea pigs
Vaccination Protocol: Hartley female guinea pigs (group 2) were vaccinated with BCG Tokyo (Sugawara et al., 2007).

50. recombinant S. typhimurium secreting M. tuberculosis ESAT-6

a. Vaccine Ontology ID:

VO_0000535

b. Type:

Live, attenuated vaccine

c. Antigen

ESAT-6 of Mycobacterium tuberculosis (Mollenkopf et al., 2001). ESAT-6 can induce IFN-gamma production and activate T cells in human peripheral blood mononuclear cells in TB patients.

d. Gene Engineering of EsxA (ESAT-6)

Type: Fusion protein with adjuvant
Description:
Detailed Gene Information: Click here.

e. Preparation

This vaccine utilizes an attenuated Salmonella typhimurium that secretes a T-cell antigen called ESAT-6 of Mycobacterium tuberculosis through the hemolysin secretion system of E. coli (Mollenkopf et al., 2001).

f. Description

ESAT-6 has been tested as a skin-test reagent in cattle and guinea pigs. The subunit vaccine which combines ESAT-6 with monophosphoryl lipid A (MPL) and dimethyl dioctadecylammonium bromide (DDA) was developed and tested for use in BALB/c female mice (Mollenkopf et al., 2001).

g. Mouse Response

Host Strain: BALB/c, female
Vaccination Protocol: Recombinant S. typhimurium was injected into tail vein of each mouse and cured with10 mg ampicillin injected intraperitoneally (i.p.). Five mice used per group. Group A received high-dose vaccine 90 days pre-challenge and was cured twice with ampicillin. Group B received low-dose recombinant vaccine 60 days pre-challenge. Group C was vaccinated at 90, 60, and 30 days pre-challenge. Group D received s.i. prime with bacteria and naked DNA 90 days pre-challenge, two DNA boosts at 60 and 30 days pre-challenge, and curing twice. Group E received the same vaccinations and challenges as in D, though in a different order. GeneGun and pcDNAesat or PIRESesat were used during vaccination (Mollenkopf et al., 2001).
Persistence:
Side Effects: Spleens were enlarged after procedure, though CFU analysis revealed that the mice were free of Salmonella bacteria (Mollenkopf et al., 2001).
Challenge Protocol: Challenge route was same as in vaccination (tail vein injection), though employed 5x10^5 CFUs of the virulent M. tuberculosis H37Rv i.v. (Mollenkopf et al., 2001)
Efficacy: A significant difference in colony-forming units was seen at 10 versus 60 days post challenge (Mollenkopf et al., 2001). S. typhimurium induced a small but signifcant degree of protection in lungs, though only small degree of protection in the liver at day 60 (Mollenkopf et al., 2001).

V. References

1. Aagaard et al., 2011: Aagaard C, Hoang T, Dietrich J, Cardona PJ, Izzo A, Dolganov G, Schoolnik GK, Cassidy JP, Billeskov R, Andersen P. A multistage tuberculosis vaccine that confers efficient protection before and after exposure. Nature medicine. 2011; 17(2); 189-194. [PubMed: 21258338].

2. Agger et al., 2002: Agger EM, Andersen P. A novel TB vaccine; towards a strategy based on our understanding of BCG failure. Vaccine. 2002 Nov 22; 21(1-2); 7-14. [PubMed: 12443657 ].

3. Aguilar et al., 2006: Aguilar LD, Infante E, Bianco MV, Cataldi A, Bigi F, Pando RH. Immunogenicity and protection induced by Mycobacterium tuberculosis mce-2 and mce-3 mutants in a Balb/c mouse model of progressive pulmonary tuberculosis. Vaccine. 2006; 24(13); 2333-2342. [PubMed: 16388878].

4. Bertholet et al., 2010: Bertholet S, Ireton GC, Ordway DJ, Windish HP, Pine SO, Kahn M, Phan T, Orme IM, Vedvick TS, Baldwin SL, Coler RN, Reed SG. A defined tuberculosis vaccine candidate boosts BCG and protects against multidrug-resistant Mycobacterium tuberculosis. Science translational medicine. 2010; 2(53); 53ra74. [PubMed: 20944089].

5. Buddle et al., 1995: Buddle BM, Keen D, Thomson A, Jowett G, McCarthy AR, Heslop J, De Lisle GW, Stanford JL, Aldwell FE. Protection of cattle from bovine tuberculosis by vaccination with BCG by the respiratory or subcutaneous route, but not by vaccination with killed Mycobacterium vaccae. Research in veterinary science. 1995; 59(1); 10-16. [PubMed: 8525078].

6. Cai et al., 2005: Cai H, Yu DH, Tian X, Zhu YX. Coadministration of interleukin 2 plasmid DNA with combined DNA vaccines significantly enhances the protective efficacy against Mycobacterium tuberculosis. DNA and cell biology. 2005; 24(10); 605-613. [PubMed: 16225391].

7. Chandra et al., 2006: Chandra S, Kaur M, Midha S, Bhatnagar R, Banerjee-Bhatnagar N. Evaluation of the ability of N-terminal fragment of lethal factor of Bacillus anthracis for delivery of Mycobacterium T cell antigen ESAT-6 into cytosol of antigen presenting cells to elicit effective cytotoxic T lymphocyte response. Biochemical and biophysical research communications. 2006 Dec 22; 351(3); 702-7. [PubMed: 17084814 ].

8. Chiong et al., 2011: Chiong E, Kesavan A, Mahendran R, Chan YH, Sng JH, Lim YK, Kamaraj R, Tan TM, Esuvaranathan K. NRAMP1 and hGPX1 gene polymorphism and response to bacillus Calmette-Guérin therapy for bladder cancer. European urology. 2011; 59(3); 430-437. [PubMed: 21163569].

9. Collins et al., 2005: Collins DM, Skou B, White S, Bassett S, Collins L, For R, Hurr K, Hotter G, de Lisle GW. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infection and immunity. 2005 Apr; 73(4); 2379-86. [PubMed: 15784584].

10. De et al., 1999: De Lisle GW, Wilson T, Collins DM, Buddle BM. Vaccination of guinea pigs with nutritionally impaired avirulent mutants of Mycobacterium bovis protects against tuberculosis. Infection and immunity. 1999 May; 67(5); 2624-6. [PubMed: 10225931 ].

11. Dou et al., 2010: Dou J, Tang Q, Yu F, Yang H, Zhao F, Xu W, Wang J, Hu W, Hu K, Liou C, Feng He X, Wang Y. Investigation of immunogenic effect of the BCG priming and Ag85A- GM-CSF boosting in Balb/c mice model. Immunobiology. 2010; 215(2); 133-142. [PubMed: 19450898].

12. Ducati et al., 2006: Ducati RG, Ruffino-Netto A, Basso LA, Santos DS. The resumption of consumption-- a review on tuberculosis. Memorias do Instituto Oswaldo Cruz. 2006 Nov; 101(7); 697-714. [PubMed: 17160276].

13. Ernst et al., 2007: Ernst JD, Trevejo-Nunez G, Banaiee N. Genomics and the evolution, pathogenesis, and diagnosis of tuberculosis. The Journal of clinical investigation. 2007 Jul; 117(7); 1738-45. [PubMed: 17607348].

14. Falero-Diaz et al., 2000: Falero-Diaz G, Challacombe S, Banerjee D, Douce G, Boyd A, Ivanyi J. Intranasal vaccination of mice against infection with Mycobacterium tuberculosis. Vaccine. 2000; 18(28); 3223-3229. [PubMed: 10869767].

15. Fletcher, 2007: Fletcher HA. Correlates of immune protection from tuberculosis. Current molecular medicine. 2007 May; 7(3); 319-25. [PubMed: 17504116].

16. Fonseca et al., 2001: Fonseca DP, Benaissa-Trouw B, van Engelen M, Kraaijeveld CA, Snippe H, Verheul AF. Induction of cell-mediated immunity against Mycobacterium tuberculosis using DNA vaccines encoding cytotoxic and helper T-cell epitopes of the 38-kilodalton protein. Infection and immunity. 2001 Aug; 69(8); 4839-45. [PubMed: 11447158].

17. FontÃ¡n et al., 2008: FontÃ¡n PA, Aris V, Alvarez ME, Ghanny S, Cheng J, Soteropoulos P, Trevani A, Pine R, Smith I. Mycobacterium tuberculosis sigma factor E regulon modulates the host inflammatory response. The Journal of infectious diseases. 2008; 198(6); 877-885. [PubMed: 18657035].

18. Gartner et al., 2008: Gartner T, Romano M, Suin V, Kalai M, Korf H, De Baetselier P, Huygen K. Immunogenicity and protective efficacy of a tuberculosis DNA vaccine co-expressing pro-apoptotic caspase-3. Vaccine. 2008; 26(11); 1458-1470. [PubMed: 18280621].

19. Geiman et al., 2004: Geiman DE, Kaushal D, Ko C, Tyagi S, Manabe YC, Schroeder BG, Fleischmann RD, Morrison NE, Converse PJ, Chen P, Bishai WR. Attenuation of late-stage disease in mice infected by the Mycobacterium tuberculosis mutant lacking the SigF alternate sigma factor and identification of SigF-dependent genes by microarray analysis. Infection and immunity. 2004; 72(3); 1733-1745. [PubMed: 14977982].

20. Goldstone et al., 2009: Goldstone RM, Goonesekera SD, Bloom BR, Sampson SL. The transcriptional regulator Rv0485 modulates the expression of a pe and ppe gene pair and is required for Mycobacterium tuberculosis virulence. Infection and immunity. 2009; 77(10); 4654-4667. [PubMed: 19651861].

21. Grode et al., 2005: Grode L, Seiler P, Baumann S, Hess J, Brinkmann V, Nasser Eddine A, Mann P, Goosmann C, Bandermann S, Smith D, Bancroft GJ, Reyrat JM, van Soolingen D, Raupach B, Kaufmann SH. Increased vaccine efficacy against tuberculosis of recombinant Mycobacterium bovis bacille Calmette-Guérin mutants that secrete listeriolysin. The Journal of clinical investigation. 2005; 115(9); 2472-2479. [PubMed: 16110326].

22. Gupta et al., 2007: Gupta UD, Katoch VM, McMurray DN. Current status of TB vaccines. Vaccine. 2007 May 10; 25(19); 3742-51. [PubMed: 17321015].

23. Hall et al., 2009: Hall LJ, Clare S, Pickard D, Clark SO, Kelly DL, El Ghany MA, Hale C, Dietrich J, Andersen P, Marsh PD, Dougan G. Characterisation of a live Salmonella vaccine stably expressing the Mycobacterium tuberculosis Ag85B-ESAT6 fusion protein. Vaccine. 2009; 27(49); 6894-6904. [PubMed: 19755145].

24. Hamasur et al., 2003: Hamasur B, Haile M, Pawlowski A, Schroder U, Williams A, Hatch G, Hall G, Marsh P, Kallenius G, Svenson SB. Mycobacterium tuberculosis arabinomannan-protein conjugates protect against tuberculosis. Vaccine. 2003 Sep 8; 21(25-26); 4081-93. [PubMed: 12922145].

25. Hernandez et al., 2010: Hernandez Pando R, Aguilar LD, Smith I, Manganelli R. Immunogenicity and protection induced by a Mycobacterium tuberculosis sigE mutant in a BALB/c mouse model of progressive pulmonary tuberculosis. Infection and immunity. 2010; 78(7); 3168-3176. [PubMed: 20457786].

26. Hess et al., 1998: Hess J, Miko D, Catic A, Lehmensiek V, Russell DG, Kaufmann SH. Mycobacterium bovis Bacille Calmette-Guerin strains secreting listeriolysin of Listeria monocytogenes. Proceedings of the National Academy of Sciences of the United States of America. 1998 Apr 28; 95(9); 5299-304. [PubMed: 9560270].

27. Hess et al., 1999: Hess J, Kaufmann SH. Live antigen carriers as tools for improved anti-tuberculosis vaccines. FEMS immunology and medical microbiology. 1999 Feb; 23(2); 165-73. [PubMed: 10076914 ].

28. Hinchey et al., 2007: Hinchey J, Lee S, Jeon BY, Basaraba RJ, Venkataswamy MM, Chen B, Chan J, Braunstein M, Orme IM, Derrick SC, Morris SL, Jacobs WR, Porcelli SA. Enhanced priming of adaptive immunity by a proapoptotic mutant of Mycobacterium tuberculosis. The Journal of clinical investigation. 2007 Aug 1; 117(8); 2279-2288. [PubMed: 17671656].

29. Hinchey et al., 2011: Hinchey J, Jeon BY, Alley H, Chen B, Goldberg M, Derrick S, Morris S, Jacobs WR Jr, Porcelli SA, Lee S. Lysine auxotrophy combined with deletion of the SecA2 gene results in a safe and highly immunogenic candidate live attenuated vaccine for tuberculosis. PloS one. 2011; 6(1); e15857. [PubMed: 21264335].

30. Hondalus et al., 2000: Hondalus MK, Bardarov S, Russell R, Chan J, Jacobs WR Jr, Bloom BR. Attenuation of and protection induced by a leucine auxotroph of Mycobacterium tuberculosis. Infection and immunity. 2000; 68(5); 2888-2898. [PubMed: 10768986].

31. Horwitz et al., 2000: Horwitz MA, Harth G, Dillon BJ, Maslesa-Galic' S. Recombinant bacillus calmette-guerin (BCG) vaccines expressing the Mycobacterium tuberculosis 30-kDa major secretory protein induce greater protective immunity against tuberculosis than conventional BCG vaccines in a highly susceptible animal model. Proceedings of the National Academy of Sciences of the United States of America. 2000 Dec 5; 97(25); 13853-8. [PubMed: 11095745].

32. Hwang et al., 2005: Hwang SA, Kruzel ML, Actor JK. Lactoferrin augments BCG vaccine efficacy to generate T helper response and subsequent protection against challenge with virulent Mycobacterium tuberculosis. International immunopharmacology. 2005; 5(3); 591-599. [PubMed: 15683854].

33. Im et al., 2007: Im EJ, Saubi N, Virgili G, Sander C, Teoh D, Gatell JM, McShane H, Joseph J, Hanke T. Vaccine Platform for Prevention of Tuberculosis and Mother-to-Child Transmission of HIV-1 through Breastfeeding. Journal of virology. 2007 Jun 27; ; . [PubMed: 17596303].

34. Infante et al., 2005: Infante E, Aguilar LD, Gicquel B, Pando RH. Immunogenicity and protective efficacy of the Mycobacterium tuberculosis fadD26 mutant. Clinical and experimental immunology. 2005; 141(1); 21-28. [PubMed: 15958066].

35. Kamath et al., 1999: Kamath AT, Feng CG, Macdonald M, Briscoe H, Britton WJ. Differential protective efficacy of DNA vaccines expressing secreted proteins of Mycobacterium tuberculosis. Infection and immunity. 1999 Apr; 67(4); 1702-7. [PubMed: 10085007].

36. Kaufmann, 2005: Kaufmann SH. Recent findings in immunology give tuberculosis vaccines a new boost. Trends in immunology. 2005 Dec; 26(12); 660-7. [PubMed: 16246622 ].

37. Khatri et al., 2014: Khatri B, Whelan A, Clifford D, Petrera A, Sander P, Vordermeier HM. BCG Î”zmp1 vaccine induces enhanced antigen specific immune responses in cattle. Vaccine. 2014; ; . [PubMed: 24394444].

38. Khera et al., 2005: Khera A, Singh R, Shakila H, Rao V, Dhar N, Narayanan PR, Parmasivan CN, Ramanathan VD, Tyagi AK. Elicitation of efficient, protective immune responses by using DNA vaccines against tuberculosis. Vaccine. 2005 Dec 1; 23(48-49); 5655-65. [PubMed: 16157425 ].

39. Kolibab et al., 2010: Kolibab K, Yang A, Derrick SC, Waldmann TA, Perera LP, Morris SL. Highly persistent and effective prime/boost regimens against tuberculosis that use a multivalent modified vaccine virus Ankara-based tuberculosis vaccine with interleukin-15 as a molecular adjuvant. Clinical and vaccine immunology : CVI. 2010; 17(5); 793-801. [PubMed: 20357059].

40. Kumar et al., 2003: Kumar P, Amara RR, Challu VK, Chadda VK, Satchidanandam V. The Apa protein of Mycobacterium tuberculosis stimulates gamma interferon-secreting CD4+ and CD8+ T cells from purified protein derivative-positive individuals and affords protection in a guinea pig model. Infection and immunity. 2003; 71(4); 1929-1937. [PubMed: 12654810].

41. Langermans et al., 2001: Langermans JA, Andersen P, van Soolingen D, Vervenne RA, Frost PA, van der Laan T, van Pinxteren LA, van den Hombergh J, Kroon S, Peekel I, Florquin S, Thomas AW. Divergent effect of bacillus Calmette-GuÃ©rin (BCG) vaccination on Mycobacterium tuberculosis infection in highly related macaque species: implications for primate models in tuberculosis vaccine research. Proceedings of the National Academy of Sciences of the United States of America. 2001; 98(20); 11497-11502. [PubMed: 11562492].

42. Li et al., 1999: Li Z, Howard A, Kelley C, Delogu G, Collins F, Morris S. Immunogenicity of DNA vaccines expressing tuberculosis proteins fused to tissue plasminogen activator signal sequences. Infection and immunity. 1999; 67(9); 4780-4786. [PubMed: 10456931].

43. Lowrie et al., 1997: Lowrie DB, Silva CL, Colston MJ, Ragno S, Tascon RE. Protection against tuberculosis by a plasmid DNA vaccine. Vaccine. 1997; 15(8); 834-838. [PubMed: 9234527].

44. Luo et al., 2004: Luo XD, Zhu DY, Chen Q, Jiang Y, Jiang S, Yang C. [A study of the protective effect of the DNA vaccine encoding tubercle antigen 85B with MPT64 in mice challenged with Mycobacterium tuberculosis]. Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases. 2004; 27(9); 611-616. [PubMed: 15498274].

45. Martin et al., 2006: Martin C, Williams A, Hernandez-Pando R, Cardona PJ, Gormley E, Bordat Y, Soto CY, Clark SO, Hatch GJ, Aguilar D, Ausina V, Gicquel B. The live Mycobacterium tuberculosis phoP mutant strain is more attenuated than BCG and confers protective immunity against tuberculosis in mice and guinea pigs. Vaccine. 2006 Apr 24; 24(17); 3408-19. [PubMed: 16564606 ].

46. McShane et al., 2005: McShane H, Pathan AA, Sander CR, Goonetilleke NP, Fletcher HA, Hill AV. Boosting BCG with MVA85A: the first candidate subunit vaccine for tuberculosis in clinical trials. Tuberculosis (Edinburgh, Scotland). 2005 Jan-Mar; 85(1-2); 47-52. [PubMed: 15687027].

47. Miki et al., 2004: Miki K, Nagata T, Tanaka T, Kim YH, Uchijima M, Ohara N, Nakamura S, Okada M, Koide Y. Induction of protective cellular immunity against Mycobacterium tuberculosis by recombinant attenuated self-destructing Listeria monocytogenes strains harboring eukaryotic expression plasmids for antigen 85 complex and MPB/MPT51. Infection and immunity. 2004; 72(4); 2014-2021. [PubMed: 15039321].

48. Mollenkopf et al., 2001: Mollenkopf HJ, Groine-Triebkorn D, Andersen P, Hess J, Kaufmann SH. Protective efficacy against tuberculosis of ESAT-6 secreted by a live Salmonella typhimurium vaccine carrier strain and expressed by naked DNA. Vaccine. 2001 Jul 16; 19(28-29); 4028-35. [PubMed: 11427279].

49. Morris et al., 2000: Morris S, Kelley C, Howard A, Li Z, Collins F. The immunogenicity of single and combination DNA vaccines against tuberculosis. Vaccine. 2000; 18(20); 2155-2163. [PubMed: 10715531].

50. Mukai et al., 2014: Mukai T, Tsukamoto Y, Maeda Y, Tamura T, Makino M. Efficient activation of human T cells of both CD4 and CD8 subsets by urease-deficient recombinant Mycobacterium bovis BCG that produced a heat shock protein 70-M. tuberculosis-derived major membrane protein II fusion protein. Clinical and vaccine immunology : CVI. 2014; 21(1); 1-11. [PubMed: 24152387].

51. Mustafa, 2009: Mustafa AS. Th1 cell reactivity and HLA-DR binding prediction for promiscuous recognition of MPT63 (Rv1926c), a major secreted protein of Mycobacterium tuberculosis. Scandinavian journal of immunology. 2009; 69(3); 213-222. [PubMed: 19281533].

52. Oksanen et al., 2013: Oksanen KE, Halfpenny NJ, Sherwood E, Harjula SK, HammarÃ©n MM, Ahava MJ, Pajula ET, Lahtinen MJ, Parikka M, RÃ¤met M. An adult zebrafish model for preclinical tuberculosis vaccine development. Vaccine. 2013; 31(45); 5202-5209. [PubMed: 24055305].

53. Olsen et al., 2001: Olsen, A.W., L.A.H. van Pinxteren, P.B Rasmussen, and P. Andersen. Protection of Mice with a Tuberculosis Subunit Vaccine Based on a Fusion Protein of Antigen 85B and ESAT-6. Infect. Immun.. 2001; 69(5); 2773-2778.

54. Palma et al., 2007: Palma C, Iona E, Giannoni F, Pardini M, Brunori L, Orefici G, Fattorini L, Cassone A. The Ag85B protein of Mycobacterium tuberculosis may turn a protective immune response induced by Ag85B-DNA vaccine into a potent but non-protective Th1 immune response in mice. Cellular microbiology. 2007; 9(6); 1455-1465. [PubMed: 17250590].

55. Palma et al., 2010: Palma C, Vendetti S, Cassone A. Role of 4-1BB receptor in the control played by CD8(+) T cells on IFN-gamma production by Mycobacterium tuberculosis antigen-specific CD4(+) T Cells. PloS one. 2010; 5(6); e11019. [PubMed: 20544034].

56. Parra et al., 2004: Parra M, Pickett T, Delogu G, Dheenadhayalan V, Debrie AS, Locht C, Brennan MJ. The mycobacterial heparin-binding hemagglutinin is a protective antigen in the mouse aerosol challenge model of tuberculosis. Infection and immunity. 2004; 72(12); 6799-6805. [PubMed: 15557600].

57. Perez et al., 2001: Perez E, Samper S, Bordas Y, Guilhot C, Gicquel B, Martin C. An essential role for phoP in Mycobacterium tuberculosis virulence. Molecular microbiology. 2001 Jul; 41(1); 179-87. [PubMed: 11454210].

58. Pinto et al., 2004: Pinto R, Saunders BM, Camacho LR, Britton WJ, Gicquel B, Triccas JA. Mycobacterium tuberculosis defective in phthiocerol dimycocerosate translocation provides greater protective immunity against tuberculosis than the existing bacille Calmette-Guérin vaccine. The Journal of infectious diseases. 2004; 189(1); 105-112. [PubMed: 14702160].

59. Randhawa et al., 2011: Randhawa AK, Shey MS, Keyser A, Peixoto B, Wells RD, de Kock M, Lerumo L, Hughes J, Hussey G, Hawkridge A, Kaplan G, Hanekom WA, Hawn TR. Association of human TLR1 and TLR6 deficiency with altered immune responses to BCG vaccination in South African infants. PLoS pathogens. 2011; 7(8); e1002174. [PubMed: 21852947].

60. Sambandamurthy et al., 2002: Sambandamurthy VK, Wang X, Chen B, Russell RG, Derrick S, Collins FM, Morris SL, Jacobs WR Jr. A pantothenate auxotroph of Mycobacterium tuberculosis is highly attenuated and protects mice against tuberculosis. Nature medicine. 2002; 8(10); 1171-1174. [PubMed: 12219086].

61. Sambandamurthy et al., 2006: Sambandamurthy VK, Derrick SC, Hsu T, Chen B, Larsen MH, Jalapathy KV, Chen M, Kim J, Porcelli SA, Chan J, Morris SL, Jacobs WR Jr. Mycobacterium tuberculosis DeltaRD1 DeltapanCD: a safe and limited replicating mutant strain that protects immunocompetent and immunocompromised mice against experimental tuberculosis. Vaccine. 2006; 24(37-39); 6309-6320. [PubMed: 16860907].

62. Singh et al., 2011: Singh VK, Srivastava V, Singh V, Rastogi N, Roy R, Shaw AK, Dwivedi AK, Srivastava R, Srivastava BS. Overexpression of Rv3097c in Mycobacterium bovis BCG abolished the efficacy of BCG vaccine to protect against Mycobacterium tuberculosis infection in mice. Vaccine. 2011; 29(29-30); 4754-4760. [PubMed: 21565242].

63. Singhal et al., 2011: Singhal A, Mathys V, Kiass M, Creusy C, Delaire B, Aliouat el M, Dartois V, Kaplan G, Bifani P. BCG induces protection against Mycobacterium tuberculosis infection in the Wistar rat model. PloS one. 2011; 6(12); e28082. [PubMed: 22162757].

64. Skeiky et al., 2004: Skeiky YA, Alderson MR, Ovendale PJ, Guderian JA, Brandt L, Dillon DC, Campos-Neto A, Lobet Y, Dalemans W, Orme IM, Reed SG. Differential immune responses and protective efficacy induced by components of a tuberculosis polyprotein vaccine, Mtb72F, delivered as naked DNA or recombinant protein. Journal of immunology (Baltimore, Md. : 1950). 2004 Jun 15; 172(12); 7618-28. [PubMed: 15187142].

65. Skeiky et al., 2005: Skeiky YA, Alderson MR, Ovendale PJ, Lobet Y, Dalemans W, Orme IM, Reed SG, Campos-Neto A. Protection of mice and guinea pigs against tuberculosis induced by immunization with a single Mycobacterium tuberculosis recombinant antigen, MTB41. Vaccine. 2005; 23(30); 3937-3945. [PubMed: 15917115].

66. Skerry et al., 2013: Skerry C, Pokkali S, Pinn M, Be NA, Harper J, Karakousis PC, Jain SK. Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in guinea pigs. PloS one. 2013; 8(6); e66310. [PubMed: 23776655].

67. Smith et al., 2001: Smith DA, Parish T, Stoker NG, Bancroft GJ. Characterization of auxotrophic mutants of Mycobacterium tuberculosis and their potential as vaccine candidates. Infection and immunity. 2001; 69(2); 1142-1150. [PubMed: 11160012].

68. Sugawara et al., 2007: Sugawara I, Li Z, Sun L, Udagawa T, Taniyama T. Recombinant BCG Tokyo (Ag85A) protects cynomolgus monkeys (Macaca fascicularis) infected with H37Rv Mycobacterium tuberculosis. Tuberculosis (Edinburgh, Scotland). 2007 Aug 24; ; . [PubMed: 17720625 ].

69. Sun et al., 2004: Sun R, Converse PJ, Ko C, Tyagi S, Morrison NE, Bishai WR. Mycobacterium tuberculosis ECF sigma factor sigC is required for lethality in mice and for the conditional expression of a defined gene set. Molecular microbiology. 2004; 52(1); 25-38. [PubMed: 15049808].

70. SWEDBERG, 1951: SWEDBERG B. Studies in experimental tuberculosis; an investigation of some problems of immunity and resistance. Acta medica Scandinavica. Supplementum. 1951; 254; 1-120. [PubMed: 14837707].

71. Sweeney et al., 2011: Sweeney KA, Dao DN, Goldberg MF, Hsu T, Venkataswamy MM, Henao-Tamayo M, Ordway D, Sellers RS, Jain P, Chen B, Chen M, Kim J, Lukose R, Chan J, Orme IM, Porcelli SA, Jacobs WR Jr. A recombinant Mycobacterium smegmatis induces potent bactericidal immunity against Mycobacterium tuberculosis. Nature medicine. 2011; 17(10); 1261-1268. [PubMed: 21892180].

72. Tanghe et al., 1999: Tanghe A, Lefèvre P, Denis O, D'Souza S, Braibant M, Lozes E, Singh M, Montgomery D, Content J, Huygen K. Immunogenicity and protective efficacy of tuberculosis DNA vaccines encoding putative phosphate transport receptors. Journal of immunology (Baltimore, Md. : 1950). 1999; 162(2); 1113-1119. [PubMed: 9916741].

73. Thom et al., 2012: Thom RE, Elmore MJ, Williams A, Andrews SC, Drobniewski F, Marsh PD, Tree JA. The expression of ferritin, lactoferrin, transferrin receptor and solute carrier family 11A1 in the host response to BCG-vaccination and Mycobacterium tuberculosis challenge. Vaccine. 2012; 30(21); 3159-3168. [PubMed: 22426328].

74. Tsenova et al., 2006: Tsenova L, Harbacheuski R, Moreira AL, Ellison E, Dalemans W, Alderson MR, Mathema B, Reed SG, Skeiky YA, Kaplan G. Evaluation of the Mtb72F polyprotein vaccine in a rabbit model of tuberculous meningitis. Infection and immunity. 2006; 74(4); 2392-2401. [PubMed: 16552069].

75. Verreck et al., 2001: Verreck FA, de Vries RR, Ottenhoff TH. Setting a course for intervening in host-pathogen interactions. Trends in immunology. 2001 Nov; 22(11); 588-90. [PubMed: 11698199 ].

76. Verreck et al., 2009: Verreck FA, Vervenne RA, Kondova I, van Kralingen KW, Remarque EJ, Braskamp G, van der Werff NM, Kersbergen A, Ottenhoff TH, Heidt PJ, Gilbert SC, Gicquel B, Hill AV, Martin C, McShane H, Thomas AW. MVA.85A boosting of BCG and an attenuated, phoP deficient M. tuberculosis vaccine both show protective efficacy against tuberculosis in rhesus macaques. PloS one. 2009; 4(4); e5264. [PubMed: 19367339].

77. Vipond et al., 2006: Vipond J, Clark SO, Hatch GJ, Vipond R, Marie Agger E, Tree JA, Williams A, Marsh PD. Re-formulation of selected DNA vaccine candidates and their evaluation as protein vaccines using a guinea pig aerosol infection model of tuberculosis. Tuberculosis (Edinburgh, Scotland). 2006 May-Jul; 86(3-4); 218-24. [PubMed: 16520093].

78. Vordermeier et al., 2004: Vordermeier HM, Rhodes SG, Dean G, Goonetilleke N, Huygen K, Hill AV, Hewinson RG, Gilbert SC. Cellular immune responses induced in cattle by heterologous prime-boost vaccination using recombinant viruses and bacille Calmette-Guerin. Immunology. 2004 Jul; 112(3); 461-70. [PubMed: 15196215].

79. Wang et al., 2004: Wang J, Thorson L, Stokes RW, Santosuosso M, Huygen K, Zganiacz A, Hitt M, Xing Z. Single mucosal, but not parenteral, immunization with recombinant adenoviral-based vaccine provides potent protection from pulmonary tuberculosis. Journal of immunology (Baltimore, Md. : 1950). 2004; 173(10); 6357-6365. [PubMed: 15528375].

80. Wang et al., 2009: Wang QL, Pan Q, Ma Y, Wang K, Sun P, Liu S, Zhang XL. An attenuated Salmonella-vectored vaccine elicits protective immunity against Mycobacterium tuberculosis. Vaccine. 2009; 27(48); 6712-6722. [PubMed: 19733584].

81. Wang et al., 2010: Wang D, Xu J, Feng Y, Liu Y, Mchenga SS, Shan F, Sasaki J, Lu C. Liposomal oral DNA vaccine (mycobacterium DNA) elicits immune response. Vaccine. 2010; 28(18); 3134-3142. [PubMed: 20197133].

82. Wang et al., 2012: Wang C, Fu R, Chen Z, Tan K, Chen L, Teng X, Lu J, Shi C, Fan X. Immunogenicity and protective efficacy of a novel recombinant BCG strain overexpressing antigens Ag85A and Ag85B. Clinical & developmental immunology. 2012; 2012; 563838. [PubMed: 22570667].

83. Wu et al., 2008: Wu Y, Woodworth JS, Shin DS, Morris S, Behar SM. Vaccine-elicited 10-kilodalton culture filtrate protein-specific CD8+ T cells are sufficient to mediate protection against Mycobacterium tuberculosis infection. Infection and immunity. 2008; 76(5); 2249-2255. [PubMed: 18332205].

84. Xu et al., 2014: Xu Y, Yang E, Wang J, Li R, Li G, Liu G, Song N, Huang Q, Kong C, Wang H. Prime-boost BCG vaccination with lentivirus-vectored and DNA-based vaccines expressing antigens Ag85B and Rv3425 improves protective efficacy against M. tuberculosis in mice. Immunology. 2014; ; . [PubMed: 24773322].

85. Zaks et al., 2006: Zaks K, Jordan M, Guth A, Sellins K, Kedl R, Izzo A, Bosio C, Dow S. Efficient immunization and cross-priming by vaccine adjuvants containing TLR3 or TLR9 agonists complexed to cationic liposomes. Journal of immunology (Baltimore, Md. : 1950). 2006; 176(12); 7335-7345. [PubMed: 16751377].