VIOLIN Logo
VO Banner
Search: for Help
About
Introduction
Statistics
VIOLIN News
Your VIOLIN
Register or Login
Submission
Tutorial
Vaccine & Components
Vaxquery
Vaxgen
VBLAST
Protegen
VirmugenDB
DNAVaxDB
CanVaxKB
Vaxjo
Vaxvec
Vevax
Huvax
Cov19VaxKB
Host Responses
VaximmutorDB
VIGET
Vaxafe
Vaxar
Vaxism
Vaccine Literature
VO-SciMiner
Litesearch
Vaxmesh
Vaxlert
Vaccine Design
Vaxign2
Vaxign
Community Efforts
Vaccine Ontology
ICoVax 2012
ICoVax 2013
Advisory Committee
Vaccine Society
Vaxperts
VaxPub
VaxCom
VaxLaw
VaxMedia
VaxMeet
VaxFund
VaxCareer
Data Exchange
V-Utilities
VIOLINML
Help & Documents
Publications
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UM Logo

Salmonella spp.

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
    4. Microbial Pathogenesis
    5. Host Ranges and Animal Models
    6. Host Protective Immunity
  2. Vaccine Related Pathogen Genes
    1. aroA
    2. aroa
    3. crp
    4. dsd
    5. Frag C
    6. HtrA
    7. TviA
    8. Ty2
    9. fsd (Defense factor)
    10. DnaJ (Protective antigen)
    11. GroEL (Protective antigen)
    12. hslU (Protective antigen)
    13. IroN (Protective antigen)
    14. sopB (Protective antigen)
    15. TviB (Protective antigen)
    16. aroA (Virmugen)
    17. AroC (Virmugen)
    18. AroD (Virmugen)
    19. asd (Virmugen)
    20. atpA (Virmugen)
    21. cbiA (Virmugen)
    22. clpP (Virmugen)
    23. clpP from S. typhimurium str. LT2 (Virmugen)
    24. cobS (Virmugen)
    25. cpxA (Virmugen)
    26. cpxR (Virmugen)
    27. crp (Virmugen)
    28. crp from S. Typhimurium (Virmugen)
    29. cya (Virmugen)
    30. fliC (Virmugen)
    31. fliD (Virmugen)
    32. galE (Virmugen)
    33. guaA (Virmugen)
    34. guaA from S. typhimurium str. LT2 (Virmugen)
    35. guaB (Virmugen)
    36. guaB from S. enteritidis str. P125109 (Virmugen)
    37. gyrA (Virmugen)
    38. hfq (Virmugen)
    39. htrA (Virmugen)
    40. lon (Virmugen)
    41. lppA (Virmugen)
    42. lppB (Virmugen)
    43. msbB (Virmugen)
    44. NmpC (Virmugen)
    45. nuoG (Virmugen)
    46. ompR (Virmugen)
    47. PhoP (Virmugen)
    48. poxA (Virmugen)
    49. relA (Virmugen)
    50. rfc (Virmugen)
    51. rpoB (Virmugen)
    52. rpoS (Virmugen)
    53. ruvB (Virmugen)
    54. spoT (Virmugen)
    55. surA (Virmugen)
    56. tolA (Virmugen)
    57. trxA (Virmugen)
    58. wecA (Virmugen)
    59. znuA (Virmugen)
    60. znuB (Virmugen)
    61. znuC (Virmugen)
  3. Vaccine Related Host Genes
    1. human IgA
    2. Ifng (Interferon gamma)
    3. IgA
    4. IgA Fc receptor
    5. IgG
    6. IgG Fc fragment
    7. IgG2b
    8. Ighg1
    9. Ighv1-9
    10. IgM
    11. IgM
    12. IgM Fc receptor
    13. IL-17
    14. IL-6
  4. Vaccine Information
    1. Aro-deleted S. Typhi
    2. Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 4109.20)
    3. Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 43T5.20)
    4. Broilact Chick Salmonella vaccine
    5. CVD 908-htrA
    6. CVD 909
    7. CVD 915
    8. Megan Egg
    9. Megan Vac1
    10. Newcastle Disease-Bronchitis Mass & Ark Types, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D7.10)
    11. Newcastle Disease-Bronchitis Mass & Ark Types, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D7.11)
    12. Newcastle-Bronchitis Mass Type, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D6.10)
    13. S. typhi DnaJ Protein Vaccine
    14. S. typhi GroEL Protein Vaccine
    15. S. typimurium Vi4072
    16. Salmonella Choleraesuis Avirulent Live Culture Vaccine (USDA: 19A1.00)
    17. Salmonella Choleraesuis Avirulent Live Culture Vaccine (USDA: 19A1.01)
    18. Salmonella DNA vaccine encoding SopB Protein
    19. Salmonella Dublin Live Culture Vaccine (USDA: 19D1.R0)
    20. Salmonella enterica fliC/guaB mutant vaccine
    21. Salmonella Enteritidis guaBA/clpP mutant vaccine
    22. Salmonella Gallinarum cbiA/cobS mutant vaccine
    23. Salmonella Gallinarum cpxR/Ion mutant vaccine
    24. Salmonella Gallinarum crp mutant vaccine
    25. Salmonella Gallinarum nuoG mutant vaccine
    26. Salmonella Gallinarum relA/spoT mutant vaccine
    27. Salmonella IroN Protein Vaccine
    28. Salmonella typhi aroA mutant vaccine
    29. Salmonella Typhi galE mutant vaccine
    30. Salmonella Typhi phoP mutant vaccine
    31. Salmonella Typhimurium asd mutant vaccine
    32. Salmonella Typhimurium asd/rfc mutant vaccine
    33. Salmonella Typhimurium atpA mutant vaccine
    34. Salmonella Typhimurium clpP mutant vaccine
    35. Salmonella Typhimurium cya/crp mutant vaccine
    36. Salmonella Typhimurium guaBA/clpP mutant vaccine
    37. Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
    38. Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
    39. Salmonella Typhimurium hfq mutant vaccine
    40. Salmonella Typhimurium htrA mutant vaccine
    41. Salmonella Typhimurium IppA/IppB/msbB
    42. Salmonella Typhimurium IppB/msbB mutant vaccine
    43. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.00)
    44. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.01)
    45. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.02)
    46. Salmonella Typhimurium ompD/nmpC mutant vaccine
    47. Salmonella Typhimurium ompR mutant vaccine
    48. Salmonella Typhimurium poxA mutant vaccine
    49. Salmonella Typhimurium rpoS mutant vaccine
    50. Salmonella Typhimurium ruvB mutant vaccine
    51. Salmonella Typhimurium surA mutant vaccine
    52. Salmonella Typhimurium tolA mutant vaccine
    53. Salmonella Typhimurium trxA mutant vaccine
    54. Salmonella Typhimurium wecA mutant vaccine
    55. Salmonella Typhimurium znuABC mutant vaccine
    56. Salmonella vacT
    57. Typherix
    58. Typhim Vi
    59. ViVaxim
    60. Vivotif
    61. Vivotif L
    62. Zoosaloral H.
  5. References
I. General Information
1. NCBI Taxonomy ID:
590
2. Disease:
Salmonellosis
3. Introduction
Salmonella serovars, like Salmonella enterica serovars Paratyphi and Typhi, cause an estimated 200,000 deaths per year. Much of it is spread by contaminated food or drinking water. Salmonella disease is mostly found in Africa, South America, and Southeast Asia. In Southeast Asia, the prevalence of typhoidal salmonellosis is high and many patients suffer relapses. Studies are being done to see why the immune system is unable to mount a lasting defense against the bacteria, and how to counteract antibiotic resistance, one of the main difficulties encountered in the protection of the Salmonella vaccine (Boyle et al., 2007).
4. Microbial Pathogenesis
Salmonella first replicates in epithelial cells. It generally targets dendritic cells and macrophages after the mucosal barrier, where it multiplies rapidly (Cheminay and Hensel, 2007). Salmonella infections induce a weak and delayed T cell responses in human typhoid fever and in animal models of typhoid fever, suggesting immunosuppressive effects. Salmonella also directly inhibits T cell proliferations (Cheminay and Hensel, 2007).
5. Host Ranges and Animal Models
S. Typhi only causes infections in humans. S. Typhimurium causes a lethal disease in susceptible mice which resembles in many aspects of human typoid fever. Therefore, S. Typhimurium has been frequently used as a model to study human typhoid fever. S. enterica causes zoonotic gastrointestinal infections from reservoirs in all important livestock animals. Vaccine studies have been done on humans, cattle, mice, and chickens (Boyle et al., 2007; Boyle et al., 2007).
6. Host Protective Immunity
Little information exists regarding the protective immunity and immunological memory for serovar Typhi. B-cell production of soluble antibodies, cytotoxic T lymphocyte activities for killing infected cells, CD4+ and CD8+ T cell homing to the gut, and distinct subsets of memory T cells are shown to be essential for protective immunity against serovar Typhi infection (Boyle et al., 2007). Two vaccines are currently commercially available against typhoid. Typhim Vi (Pasteur Merieux) is a vaccine of purified Vi-antigen from S. Typhi. Vivotif is a live attenuated S. Typhi Ty21 strain, generated by the mutagenesis of the virulent, parental S. Typhi Ty2. The major challenge of vaccine development is balancing immunogenicity and attenuation. Many vaccines have been developed with mutations in regulatory genes encoding adenylate cyclase and cAMP receptor protien (Cheminay and Hensel, 2007).
1. aroA
  • Gene Name : aroA
2. aroA
  • Gene Name : aroA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. Ty2
  • NCBI Gene ID : 1068996
  • NCBI Protein GI : 29142379
  • Locus Tag : t1956
  • Genbank Accession : AE014613
  • Protein Accession : NP_805721
  • Taxonomy ID : 209261
  • Gene Starting Position : 2006648
  • Gene Ending Position : 2007931
  • Gene Strand (Orientation) : -
  • Protein Name : 3-phosphoshikimate 1-carboxyvinyltransferase
  • Protein pI : 6.35
  • Protein Weight : 44035.02
  • Protein Length : 427
  • Protein Note : catalyzes the formation of 5-O-(1-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate and 3-phosphoshikimate in tryptophan biosynthesis
  • DNA Sequence : Show Sequence 
    >gi|29140543:2006648-2007931 Salmonella enterica subsp. enterica serovar Typhi str. Ty2 chromosome, complete genome
ATTAGGCAGGCGTACTCATCCGCGCCAGTTGTTCGAAATAATCAGGGAACGTTTTTGCGGTACATTTAGG
GTCCAGGATCGTGACTGGCGTATCGGACAGTGCGACCAGTGAGAAACACATCGCCATACGGTGGTCGTTG
TACGTGCCAATATCCGCGTGTTGGAGCTTCGCCGGCGGCGTGATACGAATATAGTCGTGCCCTTCTTCGA
CTTCAGCGCCCACTTTACGTAGCTCGGTCGCCATCGCGAACAGGCGATCGGTTTCTTTCACTCGCCAGTT
ATAAATATTGCGCAACGTCGTGGTTCCTTTCGCAAACAACGCCGTGGTGGCAATCGTCATCGCCGCATCC
GGAATATGGTTCATATCCATATCTATGGCGTGCAATTCGCCGCGCGTGCAGGCAATAAAATCATCGCCCC
AGGTAATGGTCGCGCCCATTTTCTCCAGCACATCGGCAAAACGAATATCGCCCTGCATACTTTTGCGGCC
AATCCCGGTCACTTTTACCGTGCCGCCTTTTATCGCCCCAGCGGCGAGAAAATAGGACGCTGACGAGGCA
TCGCCCTCGACCAGATAGCGACCTGGAGAGTGATACTGTTGACCGCCCTTCACGACAAATTGTTGGTAGT
GATGGTTCGCTATCTCCACGCCAAAGGTTTTCATTAAATTTAGCGTGATATCGATGTAAGGTTTTGATAC
CAGTTCGCCTTTAACGCGAATAATTGTGTCTTCAGGCGCCAGCGGCGCCGTCATCAGCAGAGCGGTCAGG
AACTGGCTGGAAACGCTACCATCAACCTCAATGTCGCCGCCGATAAAACCGCCGCGCAGACGCAGGGGCG
GATAGTTTTCCTGCTCCAGGTAATCAATATTCGCCCCACCCTGACGCAGCGAATCGACCAGATGGCCTAT
CGGACGCTCTTTCATACGCGGTTCGCCGGTTAACACTATCTCATTTTGCCCCAGACATAGCGCTGCCGCT
AACGGACGCATCGCGGTTCCGGCATTACCGAGAAACAGTTCCAGAGTGCCTGACGCGCGTAATGGGCCGC
CATTACCCGTGATATCACAGCGGGTGCGATCGGCAGAAAGGGTGTAATTGATCCCCAACGCGCTCAGGGC
ATTGAGCATATGGCGGACGTCATCGCTATCCAGCAGATTCGTCAGAACGGTTTTACCACAAGCTAAAGCC
GCCAGGAGCAAAGCACGGTTTGAAACACTTTTGGAGCCAGGTAAATTAATGGCGCCATCGACCCGCGCGA
TGGGTTGTAACGTCAGGGATTCCA
  • Protein Sequence : Show Sequence 
    >gi|29142379|ref|NP_805721.1| 3-phosphoshikimate 1-carboxyvinyltransferase [Salmonella enterica subsp. enterica serovar Typhi str. Ty2]
MESLTLQPIARVDGAINLPGSKSVSNRALLLAALACGKTVLTNLLDSDDVRHMLNALSALGINYTLSADR
TRCDITGNGGPLRASGTLELFLGNAGTAMRPLAAALCLGQNEIVLTGEPRMKERPIGHLVDSLRQGGANI
DYLEQENYPPLRLRGGFIGGDIEVDGSVSSQFLTALLMTAPLAPEDTIIRVKGELVSKPYIDITLNLMKT
FGVEIANHHYQQFVVKGGQQYHSPGRYLVEGDASSASYFLAAGAIKGGTVKVTGIGRKSMQGDIRFADVL
EKMGATITWGDDFIACTRGELHAIDMDMNHIPDAAMTIATTALFAKGTTTLRNIYNWRVKETDRLFAMAT
ELRKVGAEVEEGHDYIRITPPAKLQHADIGTYNDHRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFEQL
ARMSTPA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An aroA mutant is attenuated in mice and induced significant protection from challenge with wild type S. typhi Ty2 (Dougan et al., 1987).
  • Related Vaccine(s): Salmonella typhi aroA mutant vaccine
3. aroa
  • Gene Name : aroa
4. AroC
  • Gene Name : AroC
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi Ty2
  • NCBI Gene ID : 1069415
  • NCBI Protein GI : 29140996
  • Locus Tag : t0480
  • Genbank Accession : NC_004631.1
  • Protein Accession : NP_804338.1
  • Taxonomy ID : 28901
  • Gene Starting Position : 554066
  • Gene Ending Position : 555151
  • Gene Strand (Orientation) : +
  • Protein Name : chorismate synthase
  • Protein pI : 6.68
  • Protein Weight : 39122.2
  • Protein Length : 361
  • Protein Note : catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis
  • DNA Sequence : Show Sequence 
    >GeneID|1069415 [Salmonella enterica subsp. enterica serovar Typhi Ty2 ] 554066..555151
tggcaggaaacacaattggacaactctttcgcgtaaccactttcggcgaatcacacgggctggcgcttgg
gggtatcgtcgatggcgtgccgcccggcatcccgttgacggaggccgatctgcagcacgatctcgacaga
cgccgccctggcacctcgcgctatactactcagcgccgcgaaccggaccaggtaaaaattctctccggcg
tgtttgatggcgtgacgaccggcaccagtattggcctgctgattgaaaacaccgatcagcgctcgcagga
ctacagcgcgattaaagatgtttttcgcccgggacacgcggattacacctatgagcagaaatacggcctg
cgcgattaccgcggcggtggacgttcttccgcgcgtgaaaccgcgatgcgcgtagcggcaggggcgatcg
ccaagaaatacttggcggaaaagttcggcatcgaaatccgcggctgcctgacccagatgggcgacattcc
gctggagattaaagactggcgtcaggttgagcttaatccgttcttttgccccgatgcggacaaacttgac
gcgctggacgaactgatgcgcgcgctgaaaaaagagggtgactccatcggcgcgaaagtgacggtgatgg
cgagcggcgtgccggcagggcttggcgaaccggtatttgaccgactggatgcggacatcgcccatgcgct
gatgagcatcaatgcggtgaaaggcgtggagatcggcgaaggatttaacgtggtggcgctgcgcggcagc
cagaatcgcgatgaaatcacggcgcagggttttcagagcaaccacgctggcggcatcctcggtggcatca
gtagtgggcaacacattgtggcgcatatggcgctgaaacctacctccagcattaccgtgccgggacgtac
gatcaaccggatgggtgaagaggtcgagatgatcaccaaagggcgccacgatccgtgtgtggggattcgc
gcagtgccgatcgcagaagccatgctggcgatcgtactgatggatcacctgctgcgccatcgggcacaga
atgcggatgtaaagacagagattccacgctggtaag
  • Protein Sequence : Show Sequence 
    >gi|29140996|ref|NP_804338.1| chorismate synthase [Salmonella enterica subsp. enterica serovar Typhi Ty2 ]
MAGNTIGQLFRVTTFGESHGLALGGIVDGVPPGIPLTEADLQHDLDRRRPGTSRYTTQRREPDQVKILSG
VFDGVTTGTSIGLLIENTDQRSQDYSAIKDVFRPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAI
AKKYLAEKFGIEIRGCLTQMGDIPLEIKDWRQVELNPFFCPDADKLDALDELMRALKKEGDSIGAKVTVM
ASGVPAGLGEPVFDRLDADIAHALMSINAVKGVEIGEGFNVVALRGSQNRDEITAQGFQSNHAGGILGGI
SSGQHIVAHMALKPTSSITVPGRTINRMGEEVEMITKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRHRAQ
NADVKTEIPRW
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Inactivation of AroC results in attenuation in humans, and the deletions of both AroC and AroD provide a high level of safety against restoration of pathogenicity by recombination. An aroC/aroD-deleted derivative of S. Typhi strain Ty2 (the parent strain of Ty21a) is designated the strain "CVD 908" (Tacket and Levine, 2007).
  • Related Vaccine(s): Aro-deleted S. Typhi , CVD 908-htrA
5. AroD
  • Gene Name : AroD
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi Ty2
  • NCBI Gene ID : 1070649
  • NCBI Protein GI : 29141695
  • Locus Tag : t1231
  • Genbank Accession : NC_004631.1
  • Protein Accession : NP_805037.1
  • 3D structure: PDB ID : 1QFE
  • Taxonomy ID : 28901
  • Gene Starting Position : 1298692
  • Gene Ending Position : 1299450
  • Gene Strand (Orientation) : -
  • Protein Name : 3-dehydroquinate dehydratase
  • Protein pI : 6.23
  • Protein Weight : 27648.7
  • Protein Length : 252
  • Protein Note : catalyzes the dehydration of 3-dehydroquinate to form 3-dehydroshikimate in aromatic amino acid biosynthesis
  • DNA Sequence : Show Sequence 
    >GeneID|1070649 [Salmonella enterica subsp. enterica serovar Typhi Ty2 ] 1298692..1299450
aggcgttgtgcagaatcattaatacactgcgtagatcatttacggcgatttgccccggcgctgaagcctg
cttcaccgcgccaaacgtggcggcagagccaaacacttcccctgccagacgtgaaatgacaccctctttc
gccattgacatagtaattaccggacggtcggcgtaatgttgctgcatctccagcgtggcagtgagtaacg
ttaatacatcatgcttgctttgcggcataacggcaatcttgggaatatccgcgccgagtgcttgcatttt
acgtagccgcagaaccatttcctctgcggacggcgtctggtgaaaatcgtggttagacatcacgacataa
acattatgcgcatgggcataatcgacagtggctttaacgtcagcatcaccggtaaatagctcaagatcga
tcatatcgaccaggccgctgtcgattgcggcacgattaagcgtgagataatgctgagtggttattgtctg
ctcgccgccttctttggcgctgcggaaagtaaacagtaacggaatgtcaggcatcgcatcgcggataaca
cgcgcagcggtaagaacggattgagtcgatgcgatatccataaagtgatccacgcgccactccagaatat
cgaatgtagcttcgcggtaggccagcgcctccgctttcacgctattgatgtctcttcccatcaacgacac
gataattttgggcatcccttcgccaatgataagattttttacggtgacggttttcatgc
  • Protein Sequence : Show Sequence 
    >gi|29141695|ref|NP_805037.1| 3-dehydroquinate dehydratase [Salmonella enterica subsp. enterica serovar Typhi Ty2 ]
MKTVTVKNLIIGEGMPKIIVSLMGRDINSVKAEALAYREATFDILEWRVDHFMDIASTQSVLTAARVIRD
AMPDIPLLFTFRSAKEGGEQTITTQHYLTLNRAAIDSGLVDMIDLELFTGDADVKATVDYAHAHNVYVVM
SNHDFHQTPSAEEMVLRLRKMQALGADIPKIAVMPQSKHDVLTLLTATLEMQQHYADRPVITMSMAKEGV
ISRLAGEVFGSAATFGAVKQASAPGQIAVNDLRSVLMILHNA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Inactivation of AroD results in attenuation in humans, and the deletions of both AroC and AroD provide a high level of safety against restoration of pathogenicity by recombination. An aroC/aroD-deleted derivative of S. Typhi strain Ty2 (the parent strain of Ty21a) is designated the strain "CVD 908" (Tacket and Levine, 2007).
  • Related Vaccine(s): Aro-deleted S. Typhi
6. asd
  • Gene Name : asd
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
  • NCBI Protein GI : 267995764
  • Other Database IDs : CDD:180636
    CDD:189205
    CDD:145758
  • Taxonomy ID : 588858
  • Gene Strand (Orientation) : ?
  • Protein Name : aspartate-semialdehyde dehydrogenase
  • Protein Length : 368
  • Protein Note : aspartate-semialdehyde dehydrogenase; Reviewed
  • Protein Sequence : Show Sequence 
    >gi|267995764|gb|ACY90649.1| aspartate-semialdehyde dehydrogenase [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]
MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQFGQAAPTFGDTSTGTLQDAFDLDALKALDII
VTCQGGDYTNEIYPKLRESGWQGYWIDAASTLRMKDDAIIILDPVNQDVITDGLNNGVKTFVGGNCTVSL
MLMSLGGLFAHNLVDWVSVATYQAASGGGARHMRELLTQMGQLYGHVADELATPSSAILDIERKVTALTR
SGELPVDNFGVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTASVIPVDGLCVRVGALRCHSQAFT
IKLKKEVSIPTVEELLAAHNPWAKVVPNDRDITMRELTPAAVTGTLTTPVGRLRKLNMGPEFLSAFTVGD
QLLWGAAEPLRRMLRQLA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An asd mutant, alone or in combination with a rfc mutation, is attenuated in mice and induces significant protection from challenge with wild type Salmonella typhimurium (Piao et al., 2010).
  • Related Vaccine(s): Salmonella Typhimurium asd mutant vaccine , Salmonella Typhimurium asd/rfc mutant vaccine
7. atpA
  • Gene Name : atpA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium SL1344
  • NCBI Protein GI : 301160399
  • Locus Tag : STM3867
  • Genbank Accession : AE006468
  • Protein Accession : NP_462766
  • Other Database IDs : CDD:181753
    CDD:199133
    CDD:29998
    CDD:144044
    UniProtKB/TrEMBL: E1WDU3
  • Taxonomy ID : 216597
  • Gene Starting Position : 4076433
  • Gene Ending Position : 4077974
  • Gene Strand (Orientation) : -
  • Protein Name : ATP synthase alpha subunit
  • Protein pI : 5.78
  • Protein Weight : 51045.02
  • Protein Length : 513
  • Protein Note : F0F1 ATP synthase subunit alpha; Validated
  • DNA Sequence : Show Sequence 
    >gi|16763390:4076433-4077974 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
GTTACCAGGACTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTATA
GCCACCGGACTGGTTGATCTCTTGCATCAGCGGAGCGTGATCACGGTCGACGTAAGCCAGCAGTGCGGCT
TCGAAGCTACCGATTTTCGCCAGTTCGACATCCGCCAGATAACCACGTTCTGCTGCGAACAGAACCAGAG
ACTGCTGCGCGACGGACATCGGCGCATACTGTTTCTGTTTCAGCAGTTCGGTCACTTTCTGACCGTGGTC
AAGCTGTTTACGGGTGGCATCGTCAAGGTCGGATGCAAACTGAGAGAACGCCGCCAGTTCACGATACTGC
GCCAGCGCGGTACGAATACCACCGGACAGTTTTTTCATGATCTTGGTCTGGGCTGCGCCACCTACACGGG
ATACCGAGATACCCGGGTTAACCGCAGGACGGATACCGGCGTTAAACAGGTTAGATTCCAGGAAGATCTG
ACCATCGGTAATGGAAATTACGTTGGTCGGAACGAACGCGGAAACGTCACCTGCCTGCGTTTCGATAATC
GGCAGCGCAGTCAGTGAACCGGTTTTCCCTTTCACTTCACCTTTAGTGAAGGCTTCGACGTAGTCGGCGT
TAACACGCGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAATACGTCGCCCGGGAATGCTTCACGTCC
TGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGTTTAGACAGGTCATCGTAAATG
ATCAGCGCATCTTCGCCGCGGTCACGGAAGTATTCGCCCATTGCGCAACCGGCATACGGCGCCAGATATT
GCAGCGCAGCGGATTCGGACGCGGTCGCCACCACAACAATGGTGTTAGCCAGCGCGCCGTGCTCTTCCAG
TTTACGCACCACGTTAGAGATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATGCCGGAG
TCGCGCTGGTTGATGATGGCGTCGATAGCCAGCGCGGTTTTACCGGTCTGACGGTCACCGATGATCAGTT
CACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAGCCGGTCTGCACCGGCTGATCGAC
AGACTGACGATCGATAACGCCCGGCGCAATCGCTTCAACGGCAGAGAAGCCGTCATTATCAACCGGACCT
TTACCGTCAATTGGCGCACCCAGGGTGTTTACCACACGTCCCAGCAGGCCGCGGCCAACCGGAACTTCCA
GGATACGACCCGTACATTTAACCTTCATGCCTTCGGCAAGGTCAGCGTATGGACCCATGACTACCGCACC
CACGGAGTCGCGCTCCAGGTTCAGTGCGATAGCGTAACGGTTACCCGGCAGGGAGATCATTTCACCCTGC
ATACAATCGGCCAGGCCGTGAATGCGGATAACACCGTCACTGACAGAAACAATAGTACCTTCGTTGTGAG
CTTCGCTCACCACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTG
CA
  • Protein Sequence : Show Sequence 
    >gi|301160399|emb|CBW19925.1| ATP synthase alpha subunit [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGLADCMQGEMISLPGNRYAIALNLERDS
VGAVVMGPYADLAEGMKVKCTGRILEVPVGRGLLGRVVNTLGAPIDGKGPVDNDGFSAVEAIAPGVIDRQ
SVDQPVQTGYKAVDSMIPIGRGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVR
KLEEHGALANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLLLRRP
PGREAFPGDVFYLHSRLLERAARVNADYVEAFTKGEVKGKTGSLTALPIIETQAGDVSAFVPTNVISITD
GQIFLESNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGGIRTALAQYRELAAFSQFASDLDDATRKQ
LDHGQKVTELLKQKQYAPMSVAQQSLVLFAAERGYLADVELAKIGSFEAALLAYVDRDHAPLMQEINQSG
GYNDEIEGKLKGILDSFKATQSW
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An atpA mutant is attenuated in mice and induces significant protection from challenge with wild type S. typhimurium (Chaudhuri et al., 2009).
  • Related Vaccine(s): Salmonella Typhimurium atpA mutant vaccine
8. cbiA
  • Gene Name : cbiA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6923268
  • NCBI Protein GI : 205353181
  • Locus Tag : SG2061
  • Genbank Accession : AM933173
  • Protein Accession : YP_002226982
  • Taxonomy ID : 550538
  • Gene Starting Position : 2106950
  • Gene Ending Position : 2108329
  • Gene Strand (Orientation) : -
  • Protein Name : cobyrinic acid a,c-diamide synthase
  • Protein pI : 6.59
  • Protein Weight : 47041.13
  • Protein Length : 459
  • Protein Note : responsible for the amidation of carboxylic groups at position A and C of cobyrinic acid or hydrogenobrynic acid
  • DNA Sequence : Show Sequence 
    >gi|205351346:2106950-2108329 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
GTCATAATACGCGCCTCGCCGCCGCCAGCCAGTGCTGCAACATTTCCGGACGCTGGGCGAAATGGACGTG
CAGATAGCTGGCAAACGTGTTGCCTGTCTGCCAGCCGCCAGTCCATTCCTGTAACACGCGGCCATCGCGA
ACCTTACGACAGGCCATAACGGCGGGTGTTTCGGGGATAAAATCTGAGTAGTGAAATTCATGGCCGCGCA
CTATCTCGCCAAGCGCCGCCAATAGCGTTGGCTGCATAGCCTGCGCCTCACAGTAGCCAAAGCGGGTAAG
TCGCTTACCCATTTTGCTGTGACCGGGGATAATATTCGCCATCTGATGAATTTCCCCACCGCTATCCTCC
AGAGTGCTCCCCAGATACATCAGACCGCCACACTCCGCGTAAATCGCCACGCCGCGCTGATGCGCCGCCC
GCAAATGTTTTAACATCGCCGTATTTGCCGCCAGATCCGCGGCGTATAATTCAGGATATCCACCGCCCAG
CCAGATCATCTGACAATCAGGCAATGCGCGGTCATGCAGTGGGCTAAAACGCACAATGTTAACGCCGGCA
CGCTCCAGAAAATCGATGTTATCGGGATAATAAAAGTTAAAGGCCTCATCATCCGCCAGAGCAAGCGTCA
GCCCTGCCCCCGCTGTGTTATCGGGGCGCTCAGGCCACATTCCCGTCGGAAGCGCTGATAACACGCTCAG
CGAGAGTAGCGCATCCACATCCACTGTTTGTTCCAGCGAGGCGGCAAAATCATGCCATGATTGCTGATTG
ACGAGGGATTCTCTGGCGGTAATCAACCCCAGGTGGCGTTCAGGTAATGCGACGCCGTCGCAAGGAGGAA
CATACCCCAGTACCGGCCGTGAGCAATAATGTTCAATGGCATTTTTGAGGAGCTGATAGTGGGCGTCGCT
AGTGACGCGATTCACAATCACGCCCGCGAGGTTGAGGGTCGGGTCGAAGTGCTGAAATCCCATAACGGTA
GCCGCAAGCGACGTTGAAACAGCCTTGCCGTCCACCAGTAGGATGACCGGGCAACCAAGCTGTTTGGCCA
TCGCGGCTGTGCTGCAGTAATTGGGGTCGACGCCATAGCCGTCATACAGCCCCATGACGCCTTCAATGAC
AGCGATATCCGCCTGCCGCATTTGTTCGCAAAATAAGGCGTTGAGAACAGGAGGAGGAAGCATGAAACTG
TCAAGATTACGGGACGCCACGCCACAGATAGCGGTATGCCAGCCGGTATCAAGGTAATCTGGCCCAACTT
TAAACGGCTGTACGCGCAGCGCGCGTTTTTGCAGCAGCCTCAACAAACCAAGCGTCACCGTGGTTTTGCC
GCAACCGCTTCCTGTGCCTGCAAGAATAAATGCGTGATGCCTTGCCGCCA
  • Protein Sequence : Show Sequence 
    >gi|205353181|ref|YP_002226982.1| cobyrinic acid a,c-diamide synthase [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
MAARHHAFILAGTGSGCGKTTVTLGLLRLLQKRALRVQPFKVGPDYLDTGWHTAICGVASRNLDSFMLPP
PVLNALFCEQMRQADIAVIEGVMGLYDGYGVDPNYCSTAAMAKQLGCPVILLVDGKAVSTSLAATVMGFQ
HFDPTLNLAGVIVNRVTSDAHYQLLKNAIEHYCSRPVLGYVPPCDGVALPERHLGLITARESLVNQQSWH
DFAASLEQTVDVDALLSLSVLSALPTGMWPERPDNTAGAGLTLALADDEAFNFYYPDNIDFLERAGVNIV
RFSPLHDRALPDCQMIWLGGGYPELYAADLAANTAMLKHLRAAHQRGVAIYAECGGLMYLGSTLEDSGGE
IHQMANIIPGHSKMGKRLTRFGYCEAQAMQPTLLAALGEIVRGHEFHYSDFIPETPAVMACRKVRDGRVL
QEWTGGWQTGNTFASYLHVHFAQRPEMLQHWLAAARRVL
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A cbiA mutant, in combination with a cobS mutation, is attenuated in chickens and induces significant protection from challenge with wild type S. Gallinarum. This mutant was also able to provide some protection against S. Enteritidis (Penha et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum cbiA/cobS mutant vaccine
9. clpP
  • Gene Name : clpP
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Enteritidis str. P125109
  • NCBI Gene ID : 6950532
  • NCBI Protein GI : 207855928
  • Locus Tag : SEN0430
  • Genbank Accession : AM933172
  • Protein Accession : YP_002242579
  • Taxonomy ID : 550537
  • Gene Starting Position : 482579
  • Gene Ending Position : 483202
  • Gene Strand (Orientation) : ?
  • Protein Name : ATP-dependent Clp protease proteolytic subunit
  • Protein pI : 5.88
  • Protein Weight : 22084.77
  • Protein Length : 207
  • Protein Note : hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates
  • DNA Sequence : Show Sequence 
    >gi|207855516:482579-483202 Salmonella enterica subsp. enterica serovar Enteritidis str. P125109 chromosome, complete genome
AATGTCATACAGCGGAGAACGAGATAATTTGGCCCCTCATATGGCGCTGGTGCCGATGGTCATTGAACAG
ACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGCGTCATATTTCTGACCG
GCCAGGTCGAAGACCATATGGCTAACCTGATCGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCGGA
AAAAGATATCTATCTGTACATTAATTCTCCTGGCGGCGTAATTACTGCGGGGATGTCCATCTATGACACC
ATGCAGTTTATTAAGCCAGACGTCAGCACCATTTGTATGGGACAGGCGGCCTCTATGGGGGCGTTTCTGC
TGACTGCCGGGGCGAAAGGCAAACGTTTCTGCTTGCCGAACTCTCGCGTCATGATCCACCAGCCGCTGGG
CGGCTACCAGGGCCAGGCGACGGATATTGAAATTCACGCCCGCGAAATTTTGAAAGTAAAAGGGCGCATG
AATGAACTTATGGCGCATCATACGGGTCAATCTCTTGAGCAGATTGAACGTGATACTGAGCGCGATCGCT
TCCTCTCCGCGCCTGAAGCGGTAGAGTACGGTTTGGTTGACTCAATTTTGACCCATCGTAATTG
  • Protein Sequence : Show Sequence 
    >gi|207855928|ref|YP_002242579.1| ATP-dependent Clp protease proteolytic subunit [Salmonella enterica subsp. enterica serovar Enteritidis str. P125109]
MSYSGERDNLAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIFLTGQVEDHMANLIVAQMLFLEAENPE
KDIYLYINSPGGVITAGMSIYDTMQFIKPDVSTICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLG
GYQGQATDIEIHAREILKVKGRMNELMAHHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaBA and clpP mutant (CVD 1941) is attenuated in mice after oral immunization. This mutant is also protective, and had a vaccine efficacy of 76% against challenge with wild type Salmonella Enteritidis (Tennant et al., 2011).
  • Related Vaccine(s): Salmonella Enteritidis guaBA/clpP mutant vaccine
10. clpP from S. typhimurium str. LT2
  • Gene Name : clpP from S. typhimurium str. LT2
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1251968
  • NCBI Protein GI : 16763829
  • Locus Tag : STM0448
  • Genbank Accession : AE006468
  • Protein Accession : NP_459444
  • Taxonomy ID : 700720
  • Gene Starting Position : 503209
  • Gene Ending Position : 503832
  • Gene Strand (Orientation) : +
  • Protein Name : ATP-dependent Clp protease proteolytic subunit
  • Protein pI : 5.88
  • Protein Weight : 22084.77
  • Protein Length : 207
  • Protein Note : hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates
  • DNA Sequence : Show Sequence 
    >gi|16763390:503209-503832 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
AATGTCATACAGCGGAGAACGAGATAATTTGGCCCCTCATATGGCGCTGGTGCCGATGGTCATTGAACAG
ACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGCGTCATATTTCTGACCG
GCCAGGTCGAAGACCATATGGCTAACCTGATCGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCGGA
AAAAGATATCTATCTGTACATTAATTCTCCTGGCGGCGTAATTACTGCGGGGATGTCCATCTATGACACC
ATGCAGTTTATTAAGCCAGACGTCAGCACCATTTGTATGGGACAGGCGGCCTCTATGGGGGCGTTTCTGC
TGACTGCCGGGGCGAAAGGCAAACGTTTCTGCTTGCCGAACTCTCGCGTCATGATCCACCAGCCGCTGGG
CGGCTACCAGGGCCAGGCGACGGATATTGAAATTCACGCCCGCGAAATTTTGAAAGTAAAAGGGCGCATG
AATGAACTTATGGCGCATCATACGGGTCAATCTCTTGAGCAGATTGAACGTGATACTGAGCGCGATCGCT
TCCTCTCCGCGCCTGAAGCGGTAGAGTACGGTTTGGTTGACTCAATTTTGACCCATCGTAATTG
  • Protein Sequence : Show Sequence 
    >gi|16763829|ref|NP_459444.1| ATP-dependent Clp protease proteolytic subunit [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MSYSGERDNLAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIFLTGQVEDHMANLIVAQMLFLEAENPE
KDIYLYINSPGGVITAGMSIYDTMQFIKPDVSTICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLG
GYQGQATDIEIHAREILKVKGRMNELMAHHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A mutation in clpP (CS2007) causes hyperflagellation. These hyperflagellated bacteria are attenuated in mice, and an LD50 for CS2007 could not be determined because mice did not die after inoculation with the highest dose. CS2007 also elicited protection in mice from challenge with wild type S. typhimurium (Matsui et al., 2003).
  • Related Vaccine(s): Salmonella Typhimurium clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
11. cobS
  • Gene Name : cobS
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6923296
  • NCBI Protein GI : 205353166
  • Locus Tag : SG2041
  • Genbank Accession : AM933173
  • Protein Accession : YP_002226967
  • Taxonomy ID : 550538
  • Gene Starting Position : 2092942
  • Gene Ending Position : 2093685
  • Gene Strand (Orientation) : -
  • Protein Name : cobalamin synthase
  • Protein pI : 9.13
  • Protein Weight : 24926.05
  • Protein Length : 247
  • Protein Note : catalyzes the formation of adenosylcobalamin from Ado-cobinamide-GDP and alpha-ribazole
  • DNA Sequence : Show Sequence 
    >gi|205351346:2092942-2093685 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
CTCATAACAGAGCCAGCAGAAAGATCAATTCACCAAGTTCGATCGCCGCGCCCAGCGTATCGCCGGTTTG
ACCGCCAAGCGTACGTTTAAGCAACTGGCCAAGGATGAAAATCGCCGCGCATGTGACCACCATTGCGGCC
AACCCTTGCATACCAGGCAGTAATACCGTGGCGACGATTACCGCCAATCCCAGAGTAATGCAGGTTTGCC
GTCCGCTGACTTTACCGATAAATACATTGCCAAGCCCCTCTTCACGGGCGTAGCGATGACGGTACATCAA
TAAAACGGCGCTGCCGCGTCCGGCCGCACAGGCCGCCGCCAACGCCGCCAACATTGGCGTTCCACGTAAC
GCCAGTTCGCTGACCACCAGAATTTTTGCCAGTAGCACAAAAATAAGCGCCAGCCCGCCATGGGTTCCCA
GACGACTATCACGCATAATCTCCAGCATTCGCTCACGGCGGCGCGCGGAAAAAATACCATCGCAGGTATC
GGCCAACCCATCCAGGTGAAAACCACCGGTCAGCAACGCCAGCGCCAGAATACAGAACAACGCCGCTAGC
GGAATACCGCACCAGGGTTGCAGGAGGATGAAAATCAGGCCGCTTACCCCTCCCAGAATCAACCCGATAA
AGGGAAACATCACGATCCCACGCGAATACTGCTCGAAATCCAGTCCCTGCGACCAGCGTGACGGCACGGG
CAAGCGGCTAATAAAAGCGAGCATGGCCCAAAACAGCTTACTCA
  • Protein Sequence : Show Sequence 
    >gi|205353166|ref|YP_002226967.1| cobalamin synthase [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
MSKLFWAMLAFISRLPVPSRWSQGLDFEQYSRGIVMFPFIGLILGGVSGLIFILLQPWCGIPLAALFCIL
ALALLTGGFHLDGLADTCDGIFSARRRERMLEIMRDSRLGTHGGLALIFVLLAKILVVSELALRGTPMLA
ALAAACAAGRGSAVLLMYRHRYAREEGLGNVFIGKVSGRQTCITLGLAVIVATVLLPGMQGLAAMVVTCA
AIFILGQLLKRTLGGQTGDTLGAAIELGELIFLLALL
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A cobS mutant, in combination with a cbiA mutation, is attenuated in chickens and induces significant protection from challenge with wild type S. Gallinarum. This mutant was also able to provide some protection against S. Enteritidis (Penha et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum cbiA/cobS mutant vaccine
12. cpxA
  • Gene Name : cpxA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1255585
  • NCBI Protein GI : 16767324
  • Locus Tag : STM4058
  • Genbank Accession : AE006468
  • Protein Accession : NP_462939
  • Taxonomy ID : 700720
  • Gene Starting Position : 4268375
  • Gene Ending Position : 4269748
  • Gene Strand (Orientation) : -
  • Protein Name : two-component sensor protein
  • Protein pI : 5.57
  • Protein Weight : 49326.93
  • Protein Length : 457
  • Protein Note : part of two-component CpxA/CpxR system; senses envelope stress; upregulates a number of periplasmic folding and trafficking factors
  • DNA Sequence : Show Sequence 
    >gi|16763390:4268375-4269748 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
TTTAGGTTCGCTTGTACAGCGGTAGCCACAGCGTAAGCCGCAACCCACCCAGCGGGCTATCGTCAGCCTT
CACCCAGCCGCGGTGCTGCTGCATGGCGCTTTCGACAATCGCCAGCCCCAGCCCGGTGCCGCCAGACTCG
CGGTCGCGCGCCTCATCAGTGCGATAGAACGGACGGAAGATCTGCTCGCGGTCTTCAGGGCTGACGCCCG
GCCCGTCGTCATCGACCGTGATCGTAATACCGTCTTTATCCACCGAGAAGCCGACTTCAATCTTCGTATG
TGAATAGCGCAGCGCATTACGAACTATATTTTCCAGCGCGCTTTCCAGCGCGTTTGGGTTGCCATAGAGC
GGCCACGGCCCCGGCGGATAATTTACCGTTAACGACTTGCCCATCTGTTCGGCTTCAAAGGCGGCGTTAT
CCAGCACTTCGCCCCATAGCTGATTGGCTTTCATCGTTTCGCTGACCAGCGCATTTTTCTGTTGGTTACG
CGACATCACCAGCAGGTCATTGATCATGCTGTCCAGACGCTGCGCTTCGGTTTCAATACGCTCCAGCTCT
TTGCTTTCGCCGCCACGACGACGCAGCAGCGCGGTACCCAGTTGCAGGCGCGTAAGGGGCGTTCGTAGCT
CATGGGAGATGTCTGACAGCAAACGCTGCTGCGAGGTCATCATCCGTTCCAGCGCCGTCACCATCTGGTT
AAAACTGGCGCCAGCGGCGAGGAACTCCTGCGGACCCGCCTCCAGCTCCGGATGCTGACGCAGGTTGCCT
TGCGCCACTTCATCAGCCGCGTTTTTCAACTTACGCGCCGGTTTCGCCAGACTCCATGCCAGCCATAGCA
AGAGCGGCGAACTGACCAGCATCGTGACAATGAGCAACAGAAGCGGGCGGTCAAACAGCAGATTAATAAA
ATCGGATTGCGAACTGCTGGCCGGTCGAATCAAGTAAAGCTGGTAATTATCCTCTCCGTCGCGAACGGAG
AACGGCCCCACCATCTCTACGCGGCCATATTTTTTCTTCTGCGGATGATCGGCGTTATCCGCCTGACCAA
TGAAGTTACGAATGATCTGCATTTCGCTGCGTTCAGCGCCGATCACGCGTCCTTCAGAGGTCACCAGTAA
TAACCGCTGTCCAGGCGGCGCCCACTTATCGATCGCGCGGAACAGGCGACGCCACCACATCAGGTCGTTG
GGCGGATCGTTCGCAAGTTCAGCTTCTACATGTTGCTCTATCATCAATCCCTGGCGCTGTTCGCTGTCCA
GCAGCTCGGTCATCTGGCGTGAATCGAGCTTGGGCAACATCAGGACCAGCATTAGCACCAGCGCCAGCGT
CAACCAGAAGATGGCGAAGATGCGCGCGGTTAAACTTCCTATCA
  • Protein Sequence : Show Sequence 
    >gi|16767324|ref|NP_462939.1| two-component sensor protein [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQRQGLMIEQHVEAELANDPPNDLMWWRR
LFRAIDKWAPPGQRLLLVTSEGRVIGAERSEMQIIRNFIGQADNADHPQKKKYGRVEMVGPFSVRDGEDN
YQLYLIRPASSSQSDFINLLFDRPLLLLIVTMLVSSPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPE
LEAGPQEFLAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRGGESKELERIETE
AQRLDSMINDLLVMSRNQQKNALVSETMKANQLWGEVLDNAAFEAEQMGKSLTVNYPPGPWPLYGNPNAL
ESALENIVRNALRYSHTKIEVGFSVDKDGITITVDDDGPGVSPEDREQIFRPFYRTDEARDRESGGTGLG
LAIVESAMQQHRGWVKADDSPLGGLRLTLWLPLYKRT
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A gyrA-cpxA-rpoB Salmonella Typhimurium mutant was attenuated and protected against challenge in pigs (Roesler et al., 2004).
  • Related Vaccine(s): Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
13. cpxR
  • Gene Name : cpxR
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6923307
  • NCBI Protein GI : 205354363
  • Locus Tag : SG3362
  • Genbank Accession : AM933173
  • Protein Accession : YP_002228164
  • Taxonomy ID : 550538
  • Gene Starting Position : 3515980
  • Gene Ending Position : 3516678
  • Gene Strand (Orientation) : ?
  • Protein Name : DNA-binding transcriptional regulator CpxR
  • Protein pI : 5.38
  • Protein Weight : 25082.91
  • Protein Length : 232
  • Protein Note : response regulator in two-component regulatory system with CpxA; part of the envelope stress response system
  • DNA Sequence : Show Sequence 
    >gi|205351346:3515980-3516678 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
AATGAATAAAATCCTGTTAGTTGATGATGACCGAGAGCTGACTTCCCTGTTAAAAGAGCTCCTCGAAATG
GAAGGTTTTAATGTCCTGGTGGCCCACGACGGCGAGCAGGCGCTTGAGCTTTTGGATGACAGCATCGATT
TACTTTTGCTTGACGTCATGATGCCGAAGAAAAACGGTATCGATACGTTGAAAGCGCTTCGCCAGACACA
CCAGACGCCTGTCATTATGCTGACCGCGCGCGGCAGCGAGCTGGATCGCGTTCTCGGCCTTGAGCTGGGC
GCGGACGACTATTTGCCCAAACCGTTTAACGACCGCGAGCTGGTAGCGCGCATCAGGGCTATTTTGCGCC
GTTCCCACTGGAGCGAACAGCAGCAGAGCAGCGACAACGGCTCGCCGACGCTGGAAGTCGATGCGCTAAG
CCTTAATCCGGGTCGCCAGGAAGCCAGTTTCGATGGCCAAACGCTGGAGCTGACCGGTACGGAATTCACC
CTGCTCTATTTGCTGGCGCAACACCTCGGCCAGGTGGTTTCCCGTGAACATTTAAGCCAGGAAGTGCTGG
GTAAGCGCCTGACGCCGTTCGATCGCGCCATTGATATGCATATTTCTAACCTGCGCCGCAAACTGCCGGA
ACGCAAAGACGGTCACCCGTGGTTTAAAACATTGCGTGGTCGCGGCTATCTGATGGTTTCCGCTTCATG
  • Protein Sequence : Show Sequence 
    >gi|205354363|ref|YP_002228164.1| DNA-binding transcriptional regulator CpxR [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
MNKILLVDDDRELTSLLKELLEMEGFNVLVAHDGEQALELLDDSIDLLLLDVMMPKKNGIDTLKALRQTH
QTPVIMLTARGSELDRVLGLELGADDYLPKPFNDRELVARIRAILRRSHWSEQQQSSDNGSPTLEVDALS
LNPGRQEASFDGQTLELTGTEFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPE
RKDGHPWFKTLRGRGYLMVSAS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A cpxR mutant, in combination with a lon mutation, is highly attenuated in mice and induces significant protection from challenge with wild type S. gallinarum (Matsuda et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum cpxR/Ion mutant vaccine
14. crp
  • Gene Name : crp
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum
  • NCBI Nucleotide GI : 46811320
  • NCBI Protein GI : 46811321
  • Protein Accession : AAT01929.1
  • Taxonomy ID : 594
  • Gene Strand (Orientation) : ?
  • Protein Name : cAMP receptor protein
  • Protein Note : cyclic AMP receptor protein; CAP; catabolite gene activator protein
  • DNA Sequence : Show Sequence 
    >gi|46811320|gb|AY594269.1| Salmonella enterica subsp. enterica serovar Gallinarum cAMP receptor protein gene, complete cds
CAATCTATTTTCGTAGCCCCCTTCCCAGGTAGCGGGAAGTATATTTTTGCAACCCCAGAGACAGTGCCGT
TTTCTGGCTCTGGAGACAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACC
CGACTCTTGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCGTCAAAGAGCACGCTGATTCACCA
GGGTGAAAAAGCAGAAACGCTGTACTACATCGTTAAAGGCTCCGTGGCAGTGCTGATCAAAGATGAAGAA
GGGAAAGAAATGATCCTTTCTTATCTGAATCAGGGTGATTTTATTGGTGAACTGGGCCTGTTTGAAGAAG
GCCAGGAACGCAGCGCCTGGGTACGTGCGAAAACCGCATGTGAGGTCGCTGAAATTTCCTACAAAAAATT
TCGCCAATTAATCCAGGTCAACCCGGATATTCTGATGCTCCTCTCTTCCCAGATGGCTCGTCGCTTACAA
GTCACCTCTGAAAAAGTAGGTAACCTCGCCTTCCTTGACGTCACCGGGCGTATCGCTCAGACGCTGCTGA
ATCTGGCGAAACAGCCCGATGCCATGACGCACCCGGATGGGATGCAGATCAAAATCACCCGTCAGGAAAT
CGGCCAGATCGTCGGCTGCTCCCGCGAAACCGTTGGTCGTATTTTGAAAATGCTGGAAGATCAAAACCTG
ATCTCCGCGCATGGCAAGACCATCGTCGTCTACGGCACCCGTTAATTCCGTCAGAATGGCG
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A crp mutant is attenuated in mice and induces significant protection from challenge with wild type S. gallinarum (Rosu et al., 2007).
  • Related Vaccine(s): Salmonella Gallinarum crp mutant vaccine
15. crp
  • Gene Name : crp
  • Taxonomy ID : 99287
16. crp from S. Typhimurium
  • Gene Name : crp from S. Typhimurium
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1254989
  • NCBI Protein GI : 16766754
  • Locus Tag : STM3466
  • Genbank Accession : AE006468
  • Protein Accession : NP_462369
  • Taxonomy ID : 99287
  • Gene Starting Position : 3615644
  • Gene Ending Position : 3616276
  • Gene Strand (Orientation) : +
  • Protein Name : cAMP-regulatory protein
  • Protein pI : 8.46
  • Protein Weight : 22267.2
  • Protein Length : 210
  • Protein Note : complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons
  • DNA Sequence : Show Sequence 
    >gi|16763390:3615644-3616276 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
CATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTTGAATGGTTCTTGTCTCATTGCCACATTCATAAG
TACCCGTCAAAGAGCACGCTGATTCACCAGGGTGAAAAAGCAGAAACGCTGTACTACATCGTTAAAGGCT
CCGTGGCAGTGCTGATCAAAGATGAAGAAGGGAAAGAAATGATCCTTTCTTATCTGAATCAGGGTGATTT
TATTGGTGAACTGGGCCTGTTTGAAGAAGGCCAGGAACGCAGCGCCTGGGTACGTGCGAAAACCGCATGT
GAGGTCGCTGAAATTTCCTACAAAAAATTTCGCCAATTAATCCAGGTCAACCCGGATATTCTGATGCGCC
TCTCTTCCCAGATGGCTCGTCGCTTACAAGTCACCTCTGAAAAAGTAGGTAACCTCGCCTTCCTTGACGT
CACCGGGCGTATCGCTCAGACGCTGCTGAATCTGGCGAAACAGCCCGATGCCATGACGCACCCGGATGGG
ATGCAGATCAAAATCACTCGTCAGGAAATCGGCCAGATCGTCGGCTGCTCCCGCGAAACCGTTGGTCGTA
TTTTGAAAATGCTGGAAGATCAAAACCTGATCTCCGCGCATGGCAAGACCATCGTCGTCTACGGCACCCG
TTA
  • Protein Sequence : Show Sequence 
    >gi|16766754|ref|NP_462369.1| cAMP-regulatory protein [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDF
IGELGLFEEGQERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSSQMARRLQVTSEKVGNLAFLDV
TGRIAQTLLNLAKQPDAMTHPDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A cya and crp mutant is attenuated in mice. Thirty days after immunization, mice were protected from oral challenge with wild type S. typhimurium (Curtiss and Kelly, 1987).
  • Related Vaccine(s): Salmonella Typhimurium cya/crp mutant vaccine
17. cya
  • Gene Name : cya
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • NCBI Protein GI : 581756
  • Other Database IDs : CDD:193111
    GOA:P0A1A7
    InterPro: IPR000274
    UniProtKB/UniProt: P0A1A7
  • Taxonomy ID : 90371
  • Gene Strand (Orientation) : ?
  • Protein Name : adenylate cyclase
  • Protein Length : 29
  • Protein Note : Adenylate cyclase NT domain; pfam12633
  • Protein Sequence : Show Sequence 
    >gi|581756|emb|CAA39304.1| adenylate cyclase [Salmonella enterica subsp. enterica serovar Typhimurium]
MYLYIETLKQRLDAINQLRVDRALAAMGP
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A cya and crp mutant is attenuated in mice. Thirty days after immunization, mice were protected from oral challenge with wild type S. typhimurium (Curtiss and Kelly, 1987).
  • Related Vaccine(s): Salmonella Typhimurium cya/crp mutant vaccine
18. DnaJ
  • Gene Name : DnaJ
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. CT18
  • VO ID : VO_0011268
  • NCBI Gene ID : 1246524
  • NCBI Protein GI : 16759006
  • Locus Tag : STY0013
  • Genbank Accession : AL513382
  • Protein Accession : NP_454623
  • Taxonomy ID : 220341
  • Gene Starting Position : 13595
  • Gene Ending Position : 14734
  • Gene Strand (Orientation) : +
  • Protein Name : DnaJ protein
  • Protein pI : 8.26
  • Protein Weight : 38831.98
  • Protein Length : 379
  • Protein Note : chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion
  • DNA Sequence : Show Sequence 
    >NC_003198.1:13595-14734 Salmonella enterica subsp. enterica serovar Typhi str. CT18, complete genome
GATGGCGAAAAGAGATTACTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAAAAAG
GCGTATAAGCGCCTGGCCATGAAATATCATCCGGACCGCAATCAGGGTGATAAAGAGGCCGAAGCTAAGT
TTAAAGAGATTAAAGAAGCCTACGAAGTGCTGACCGATGCGCAAAAACGCGCAGCCTACGATCAGTATGG
TCACGCCGCGTTTGAACAAGGCGGGATGGGCGGCGGATTTGGCGGCGGCTTTAATGGCGGCGCTGATTTC
AGTGATATCTTTGGTGACGTTTTTGGCGATATCTTTGGCGGCGGGCGTGGTCGCCAACGTGCGGCGCGTG
GGGCTGATTTGCGTTATAACATGGATCTCACCCTGGAAGAAGCGGTGCGTGGCGTGACCAAAGAGATCCG
TATTCCGACGCTGGAGGAGTGCGACGTTTGCCACGGCAGCGGCGCGAAAGCTGGTACGCAACCGCAAACC
TGTCCGACCTGTCATGGTTCTGGTCAGGTACAGATGCGCCAGGGATTCTTTGCTGTACAGCAGACCTGCC
CACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCCATAAATGTCACGGTCATGGGCGTGTTGA
AAAGAGTAAAACTCTGTCCGTTAAAATCCCGGCGGGCGTGGATACCGGCGATCGTATTCGTCTGGCAGGC
GAGGGCGAAGCGGGCGAGCATGGCGCACCGGCAGGCGACTTGTACGTTCAGGTCCAGGTGAAACAACACC
CTATTTTCGAGCGTGAAGGCAATAATCTTTATTGCGAAGTGCCGATCAACTTTGCGATGGCGGCGCTCGG
CGGTGAAATTGAAGTGCCGACGTTAGATGGTCGCGTGATGCTGAAAGTACCGAGCGAAACACAAACGGGC
AAGCTGTTCCGTATGCGCGGCAAAGGCGTGAAGTCCGTACGCGGTGGCGCGCAGGGCGATTTGCTGTGCC
GTGTGGTAGTTGAAACGCCGGTCGGTCTGAGCGAAAAACAGAAGCAATTGCTAAAAGATCTTCAGGAAAG
TTTTGGCGGCCCGACGGGAGAGAAAAACAGCCCGCGTTCAAAAAGCTTCTTTGACGGCGTGAAAAAATTC
TTTGACGATTTGACTCGCTA
  • Protein Sequence : Show Sequence 
    >NP_454623.1 DnaJ protein [Salmonella enterica subsp. enterica serovar Typhi str. CT18]
MAKRDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAEAKFKEIKEAYEVLTDAQKRAAYDQYG
HAAFEQGGMGGGFGGGFNGGADFSDIFGDVFGDIFGGGRGRQRAARGADLRYNMDLTLEEAVRGVTKEIR
IPTLEECDVCHGSGAKAGTQPQTCPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCHKCHGHGRVE
KSKTLSVKIPAGVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAALG
GEIEVPTLDGRVMLKVPSETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLSEKQKQLLKDLQES
FGGPTGEKNSPRSKSFFDGVKKFFDDLTR
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Immunisation of mice with DnaJ derived from Salmonella enterica subsp. enterica serovar Typhi str. CT18 was found to provide 70% protection against lethal challenge by S. Typhimurium indicating the possible use of DnaJ as vaccine candidate against typhoid (Sagi et al., 2006).
  • Related Vaccine(s): S. typhi DnaJ Protein Vaccine
19. dsd
  • Gene Name : dsd
20. fliC
  • Gene Name : fliC
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Enteritidis
  • NCBI Protein GI : 119351647
  • Other Database IDs : CDD:181201
  • Taxonomy ID : 149539
  • Gene Strand (Orientation) : ?
  • Protein Name : FliC
  • Protein Length : 157
  • Protein Note : PT1
  • Protein Sequence : Show Sequence 
    >gi|119351647|gb|ABL63539.1| FliC [Salmonella enterica subsp. enterica serovar Enteritidis]
GETITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKNVTGYDTYAAGADKYRVDINSGAVVTDAAAP
DKVYVNAANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGDD
GNGKVSTTINGEKVTLT
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A fliC mutant, in combination with a guaB mutant, is attenuated in mice and chickens and induces significant protection from challenge with wild type S. enterica (Adriaensen et al., 2007).
  • Related Vaccine(s): Salmonella enterica fliC/guaB mutant vaccine
21. fliD
  • Gene Name : fliD
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253481
  • NCBI Protein GI : 16765298
  • Locus Tag : STM1960
  • Genbank Accession : AE006468
  • Protein Accession : NP_460913
  • Taxonomy ID : 700720
  • Gene Starting Position : 2049401
  • Gene Ending Position : 2050804
  • Gene Strand (Orientation) : +
  • Protein Name : flagellar capping protein
  • Protein pI : 5.02
  • Protein Weight : 47254.05
  • Protein Length : 467
  • Protein Note : involved in flagellin assembly
  • DNA Sequence : Show Sequence 
    >gi|16763390:2049401-2050804 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
CATGGCTTCAATTTCATCATTAGGTGTGGGATCAAACTTACCGTTAGACCAGTTGTTGACAGACCTGACA
AAGAACGAAAAAGGACGCTTAACGCCAATTACCAAACAGCAGAGCGCGAATTCGGCAAAGCTAACCGCCT
ATGGCACATTGAAAAGCGCATTAGAAAAATTCCAGACGGCAAATACCGCGTTAAATAAAGCGGATTTATT
TAAGTCTACCGTGGCGTCCAGCACCACTGAAGATCTCAAAGTCAGTACTACCGCTGGCGCTGCCGCAGGG
ACTTATAAGATTAACGTAACCCAGCTTGCCGCCGCACAGTCGCTGGCGACAAAAACCACCTTCGCGACCA
CCAAAGAGCAGTTGGGCGATACGTCGGTCACGTCCCGGACAATTAAAATTGAACAGCCGGGACGTAAAGA
GCCGCTGGAAATTAAGCTGGATAAAGGCGACACCTCCATGGAGGCGATCCGTGACGCCATCAATGACGCC
GACAGCGGTATCGCCGCCAGTATCGTTAAGGTCAAAGAGAACGAATTCCAGTTGGTGCTTACCGCCAATA
GCGGTACCGACAATACGATGAAGATCACGGTGGAAGGCGATACAAAACTTAACGATCTACTCGCTTATGA
CAGCACCACCAATACCGGCAATATGCAAGAGCTGGTGAAAGCAGAAAACGCGAAGCTGAACGTAAACGGC
ATCGACATTGAGCGTCAGAGCAATACCGTAACCGACGCCCCTCAGGGAATTACGCTCACCCTGACCAAGA
AAGTGACCGACGCGACCGTGACGGTAACGAAAGATGATACCAAGGCGAAAGAGGCGATTAAATCCTGGGT
GGATGCCTATAACTCGCTGGTGGATACCTTTAGCTCGTTAACCAAATATACCGCCGTTGAGCCGGGCGAA
GAAGCCAGCGATAAAAACGGCGCGCTGTTAGGCGATAGTGTGGTTCGTACTATCCAGACCGGGATTCGGG
CACAATTTGCCAATAGCGGCAGTAATTCTGCGTTCAAAACAATGGCGGAAATTGGCATCACCCAGGATGG
GACTTCCGGCAAACTGAAGATTGATGATGATAAGCTGACCAAAGTACTGAAAGATAACACAGCCGCAGCG
CGTGAGCTGCTGGTAGGCGATGGTAAAGAAACGGGTATCACCACCAAAATTGCCACCGAAGTGAAAAGTT
ATCTGGCGGATGACGGCATTATTGATAATGCGCAGGACAACGTTAACGCCACGCTGAAAAGCCTGACAAA
ACAGTACCTGTCCGTTAGCAACAGCATCGATGAAACCGTTGCCCGTTACAAGGCCCAGTTTACCCAACTG
GATACCATGATGAGTAAGCTGAATAACACCAGTAGTTATTTGACCCAGCAATTTACAGCTATGAACAAGT
CCTG
  • Protein Sequence : Show Sequence 
    >gi|16765298|ref|NP_460913.1| flagellar capping protein [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MASISSLGVGSNLPLDQLLTDLTKNEKGRLTPITKQQSANSAKLTAYGTLKSALEKFQTANTALNKADLF
KSTVASSTTEDLKVSTTAGAAAGTYKINVTQLAAAQSLATKTTFATTKEQLGDTSVTSRTIKIEQPGRKE
PLEIKLDKGDTSMEAIRDAINDADSGIAASIVKVKENEFQLVLTANSGTDNTMKITVEGDTKLNDLLAYD
STTNTGNMQELVKAENAKLNVNGIDIERQSNTVTDAPQGITLTLTKKVTDATVTVTKDDTKAKEAIKSWV
DAYNSLVDTFSSLTKYTAVEPGEEASDKNGALLGDSVVRTIQTGIRAQFANSGSNSAFKTMAEIGITQDG
TSGKLKIDDDKLTKVLKDNTAAARELLVGDGKETGITTKIATEVKSYLADDGIIDNAQDNVNATLKSLTK
QYLSVSNSIDETVARYKAQFTQLDTMMSKLNNTSSYLTQQFTAMNKS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaBA, clpP, and fliD mutant (CVD 1923) is attenuated in mice after oral immunization. This mutant is also protective, with a vaccine efficacy of 80% in mice against wild type challenge from Salmonella Typhimurium (Tennant et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
22. Frag C
  • Gene Name : Frag C
  • Sequence Strain (Species/Organism) : Escherichia coli O157:H7 EDL933
  • NCBI Gene ID : 958167
  • NCBI Protein GI : 15803220
  • Locus Tag : Z4009
  • Genbank Accession : NC_002655.2
  • Protein Accession : NP_289252.1
  • Taxonomy ID : 562
  • Gene Starting Position : 3617920
  • Gene Ending Position : 3618570
  • Gene Strand (Orientation) : +
  • Protein Name : PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components; frag
  • Protein pI : 4.79
  • Protein Weight : 22425.1
  • Protein Length : 216
  • Protein Sequence : Show Sequence 
    >gi|15803220|ref|NP_289252.1| PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components; frag [Escherichia coli O157:H7 EDL933 ]
MEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVAVLFQSGRDTIDTV
LKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLALICSFPLLSPFLGPGAVIAQVIGVL
IGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARZDTVRVGVPSVLVSRFLTGAPTVLIAWFV
SGFIYQ
  • Related Vaccine(s): CVD 915
23. fsd
  • Gene Name : fsd
  • Molecule Role : Defense factor
24. galE
  • Gene Name : galE
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. Ty2
  • NCBI Gene ID : 1067209
  • NCBI Protein GI : 29142526
  • Locus Tag : t2111
  • Genbank Accession : AE014613
  • Protein Accession : NP_805868
  • Taxonomy ID : 209261
  • Gene Starting Position : 2174895
  • Gene Ending Position : 2175911
  • Gene Strand (Orientation) : +
  • Protein Name : UDP-galactose-4-epimerase
  • Protein pI : 6.18
  • Protein Weight : 34020.42
  • Protein Length : 338
  • Protein Note : corresponds to STY0809 from Accession AL513382: Salmonella typhi CT18
  • DNA Sequence : Show Sequence 
    >gi|29140543:2174895-2175911 Salmonella enterica subsp. enterica serovar Typhi str. Ty2 chromosome, complete genome
TATGAGAGTATTGGTTACAGGTGGTAGCGGTTACATTGGAAGTCATACCTGCGTACAATTGCTGCAAAAT
GGTCATGACGTCGTCATCCTCGATAACCTCTGCAACAGCAAGCGCAGCGTGCTGCCCGTTATTGAACGTC
TGGGCGGTAAGCACCCGACCTTTGTCGAAGGCGATATTCGCAACGAAGCGCTTATAACCGAAATTCTGCA
CGATCACGCGATTGATACCGTGATTCACTTTGCCGGCCTGAAAGCCGTCGGCGAATCGGTCGCCAAGCCG
CTGGAGTACTATGACAACAACGTCAACGGTACGCTGCGGTTGGTCAGCGCCATGCGTGCGGCCAACGTTA
AAAACCTTATTTTTAGCTCCTCTGCCACCGTTTATGGCGATCAGCCCAAAATTCCTTACGTCGAAAGTTT
TCCTACCGGCACGCCGCAAAGCCCCTACGGCAAAAGTAAATTGATGGTAGAACAGATCCTCACCGATCTG
CAAAAAGCCCAGCCGGAGTGGAGTATTGCGCTGCTGCGTTATTTCAATCCGGTCGGCGCGCACCCGTCGG
GCGACATGGGAGAAGATCCGCAAGGTATTCCGAATAACCTGATGCCCTATATCGCCCAGGTCGCCGTGGG
TCGTCGCGAATCGCTCGCCGTTTTCGGCAACGATTACCCGACCGAGGATGGCACCGGCGTACGCGATTAC
ATTCACGTTATGGACTTAGCCGACGGGCACGTCGTGGCGATGGAAAAACTGGCGGACAAATCCGGCGTAC
ATATTTATAACCTCGGCGCGGGTGTCGGCAGTAGCGTCCTGGATGTGGTCAACGCCTTTAGTAAAGCCTG
CGGTAAACCCATTAATTACCACTTCGCGCCGCGTCGCGACGGCGATCTCCCGGCGTATTGGGCGGATGCC
AGCAAAGCCGATCGCGAGCTGAACTGGCGCGTCACCCGCACGCTTGACGAAATGGCGCAGGACACTTGGC
ACTGGCAGTCACGCCATCCGCAGGGATACTCAGATTA
  • Protein Sequence : Show Sequence 
    >gi|29142526|ref|NP_805868.1| UDP-galactose-4-epimerase [Salmonella enterica subsp. enterica serovar Typhi str. Ty2]
MRVLVTGGSGYIGSHTCVQLLQNGHDVVILDNLCNSKRSVLPVIERLGGKHPTFVEGDIRNEALITEILH
DHAIDTVIHFAGLKAVGESVAKPLEYYDNNVNGTLRLVSAMRAANVKNLIFSSSATVYGDQPKIPYVESF
PTGTPQSPYGKSKLMVEQILTDLQKAQPEWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVG
RRESLAVFGNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLADKSGVHIYNLGAGVGSSVLDVVNAFSKAC
GKPINYHFAPRRDGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQGYSD
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A gal E mutant in strain Ty2 was isolated, attenuated, and protected mice against lethal challenge (Germanier and Füer, 1975).
  • Related Vaccine(s): Salmonella Typhi galE mutant vaccine
25. GroEL
  • Gene Name : GroEL
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. Ty2
  • VO ID : VO_0010998
  • NCBI Gene ID : 1068458
  • NCBI Protein GI : 29144631
  • Locus Tag : t4382
  • Genbank Accession : AE014613
  • Protein Accession : NP_807973
  • Taxonomy ID : 209261
  • Gene Starting Position : 4534506
  • Gene Ending Position : 4536152
  • Gene Strand (Orientation) : +
  • Protein Name : molecular chaperone GroEL
  • Protein pI : 4.57
  • Protein Weight : 51532.68
  • Protein Length : 548
  • Protein Note : 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth
  • DNA Sequence : Show Sequence 
    >NC_004631.1:4534506-4536152 Salmonella enterica subsp. enterica serovar Typhi Ty2, complete genome
AATGGCAGCTAAAGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTG
GCAGATGCAGTGAAAGTAACCCTCGGTCCGAAAGGCCGTAACGTGGTTCTGGATAAATCTTTCGGTGCGC
CGACTATCACTAAAGATGGTGTTTCCGTAGCGCGTGAAATCGAGCTGGAAGACAAGTTTGAAAACATGGG
CGCGCAGATGGTGAAAGAAGTTGCCTCTAAAGCGAACGACGCTGCAGGCGACGGCACCACCACCGCGACC
GTACTGGCGCAGTCCATCATTACCGAAGGCCTGAAAGCCGTTGCTGCGGGCATGAACCCGATGGACCTGA
AACGTGGTATCGACAAAGCGGTTGCTGCTGCGGTTGAAGAGCTGAAGGCGCTGTCCGTACCGTGCTCCGA
CTCTAAAGCGATTGCTCAGGTAGGTACTATTTCCGCTAACTCCGACGAAACCGTAGGTAAACTGATCGCG
GAAGCGATGGATAAAGTCGGTAAAGAAGGCGTCATCACTGTTGAAGACGGTACCGGTCTGCAGGACGAAC
TGGACGTGGTTGAAGGTATGCAGTTTGACCGCGGCTACCTGTCTCCTTACTTCATCAACAAGCCGGAAAC
TGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGATAAGAAAATCTCCAACATCCGCGAAATG
CTGCCGGTTCTGGAAGCCGTTGCAAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTTGAAGGCG
AAGCGCTGGCTACCCTGGTAGTGAACACCATGCGTGGCATCGTGAAAGTGGCTGCGGTTAAAGCACCGGG
CTTCGGCGATCGTCGTAAGGCGATGCTGCAGGATATCGCTACCCTGACCGGCGGTACCGTAATCTCTGAA
GAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCGAAACGTGTTGTGATCAACA
AAGACACCACCACCATCATTGATGGCGTGGGTGAAGAAGCTGCCATCCAGGGCCGTGTTGCTCAGATCCG
TCAGCAGATTGAAGAAGCGACCTCCGACTACGATCGTGAAAAACTGCAGGAGCGCGTAGCGAAACTGGCA
GGCGGCGTTGCGGTAATCAAAGTTGGCGCTGCGACCGAAGTTGAAATGAAAGAGAAGAAAGCCCGCGTTG
AAGATGCCCTGCACGCGACCCGTGCTGCGGTAGAAGAAGGCGTGGTTGCTGGTGGTGGCGTTGCGCTGAT
CCGCGTTGCTTCTAAAATTGCTGACCTGAAAGGCCAGAACGAAGACCAGAACGTGGGTATCAAAGTTGCG
CTGCGCGCAATGGAAGCTCCGCTGCGTCAGATCGTGCTGAACTGCGGCGAAGAGCCGTCTGTTGTCGCTA
ACACCGTTAAAGGCGGCGACGGTAACTACGGTTACAACGCAGCAACTGAAGAATACGGCAACATGATCGA
TATGGGTATCTTGGACCCAACCAAAGTTACCCGTTCTGCGCTGCAATACGCGGCTTCTGTGGCTGGTCTG
ATGATCACTACCGAGTGCATGGTGACCGACCTGCCGAAAAGCGATGCTCCTGATTTAGGCGCTGCTGGCG
GCATGGGTGGTATGGGTGGTATGGGCGGCATGATGTA
  • Protein Sequence : Show Sequence 
    >NP_807973.1 molecular chaperone GroEL [Salmonella enterica subsp. enterica serovar Typhi str. Ty2]
MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMG
AQMVKEVASKANDAAGDGTTTATVLAQSIITEGLKAVAAGMNPMDLKRGIDKAVAAAVEELKALSVPCSD
SKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPET
GAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPG
FGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIR
QQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALI
RVASKIADLKGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMID
MGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKSDAPDLGAAGGMGGMGGMGGMM
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Researchers evaluated the immunogenicity and protective efficacy of GroEL of Salmonella enterica serovar Typhi against lethal infection by S. typhi Ty2 in mice with or without adjuvants. Immunization of mice with GroEL-alone, GroEL + alum, and GroEL + CFA provided 70, 50 and 80% protection, respectively, against lethal challenge by S. typhi in mice. The present study shows that GroEL forms an ideal candidate molecule to develop a recombinant protein based vaccine against human typhoid (Bansal et al., 2010).
  • Related Vaccine(s): S. typhi GroEL Protein Vaccine
26. guaA
  • Gene Name : guaA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Enteritidis str. P125109
  • NCBI Gene ID : 6948075
  • NCBI Protein GI : 207857920
  • Locus Tag : SEN2490
  • Genbank Accession : AM933172
  • Protein Accession : YP_002244571
  • Taxonomy ID : 550537
  • Gene Starting Position : 2623837
  • Gene Ending Position : 2625414
  • Gene Strand (Orientation) : -
  • Protein Name : GMP synthase
  • Protein pI : 5.06
  • Protein Weight : 53428.72
  • Protein Length : 525
  • Protein Note : contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway
  • DNA Sequence : Show Sequence 
    >gi|207855516:2623837-2625414 Salmonella enterica subsp. enterica serovar Enteritidis str. P125109 chromosome, complete genome
CTTATTCCCACTCAATGGTAGCTGGTGGTTTACCGCTGATGTCATACACCACACGGGAAATCCCGTTGAC
TTCATTGATGATGCGGTTGGAAACACGACCCAGGAAATCATACGGCAGATGTGCCCAGTGTGCGGTCATA
AAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAATCGTACTTACGACCATCGCCCATCACGCCTA
CGGAACGGACCGGCAGGAAGACGGTAAACGCCTGACTGACTTTGTCGTACAGATCCGCTTTGCGCAGCTC
TTCAATGAAGATAGCGTCCGCGCGACGCAGCAGGTCGCAGTACTCTTTCTTCACTTCGCCCAGTACGCGC
ACGCCGAGGCCCGGCCCCGGGAACGGATGGCGATACAGCATGTCGTACGGCAGGCCCAGCTCCAGACCAA
TCTTACGCACTTCGTCTTTGAACAGCTCTTTCAGCGGTTCAACCAGCCCCATCTTCATCTCTTTCGGCAG
GCCGCCGACATTGTGGTGAGATTTGATGACGTGCGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACG
TCAGGGTAGATGGTGCCCTGCGCCAGCCATTTCACGTCTTCCAGTTTCAGCGCTTCTTCGTCGAACACTT
CAACGAAAACGCGGCCGATGATCTTACGCTTGGCTTCCGGATCGTTCTCACCAGCCAGCGCGGACAGGAA
GCGATCTTCTGCCGGTACGTGAACAATGTTCAGACCAAAATGGTCGCCAAACATGTCCATCACCTGCTCG
GCTTCGTTCAGGCGCAACAGGCCGTTGTCGACGAAAACACAGGTCAGATTTTTACCGATCGCGCGGTGCA
GCAGCATGGCGGTGACGGAAGAATCCACGCCGCCGGAGAGACCGAGGATCACTTTATCGTCGCCTACCTG
CTCGCGAATGCGCGCCACGGCGTCATCGATGATCTTCGCCGGCGTCCACAACGCTTCACACTGGCAGATA
TCGCGTACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTGTGAGTCACTTCCGGGTGGAACTGTACGC
CGTAGAAGCGTTTTTCTTCGTTGGCCATAATGGCGAACGGGCAGCTCTCGGTGCTGGCGACGGTCACGAA
GTCGGACGGAATCGCCGTCACTTTATCGCCGTGGCTCATCCACACGTCCAGCAGCGGTTTGCCGTCTGCG
GTCAGGGAATCTTCAATACCGCGAACCAGCGCGCTGTCGGTCAACACTTCGACCTGCGCATAACCAAATT
CACGCTCATTAGAACCTTCTACATGACCGCCAAGCTGCATCGCCATGGTCTGCATACCATAGCAAACGCC
AAATACCGGCACGCCTGCTTCAAAGACATACTGCGGCGCGCGCGGGCTGTTTTCTTCGGTGGTGCTTTCC
GGGCCGCCGGAAAGAATAATGCCGCTTGGGTTGAAGTCACGAATTTGTGCTTCTGTCACATCCCACGCCC
ACAGTTCACAGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTC
CAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCA
  • Protein Sequence : Show Sequence 
    >gi|207857920|ref|YP_002244571.1| GMP synthase [Salmonella enterica subsp. enterica serovar Enteritidis str. P125109]
MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEAQIRDFNPSGIILSGGPESTTEENSPR
APQYVFEAGVPVFGVCYGMQTMAMQLGGHVEGSNEREFGYAQVEVLTDSALVRGIEDSLTADGKPLLDVW
MSHGDKVTAIPSDFVTVASTESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTP
AKIIDDAVARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVMDMFGDH
FGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQGTIYPDVIESAASATG
KAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELGLPYDMLYRHPFPGPGLGVRVLGEVKKEY
CDLLRRADAIFIEELRKADLYDKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPY
DFLGRVSNRIINEVNGISRVVYDISGKPPATIEWE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaBA and clpP mutant (CVD 1941) is attenuated in mice after oral immunization. This mutant is also protective in mice and had a vaccine efficacy of 76% against challenge with wild type Salmonella Enteritidis (Tennant et al., 2011).
  • Related Vaccine(s): Salmonella Enteritidis guaBA/clpP mutant vaccine
27. guaA from S. typhimurium str. LT2
  • Gene Name : guaA from S. typhimurium str. LT2
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1254032
  • NCBI Protein GI : 16765830
  • Locus Tag : STM2510
  • Genbank Accession : AE006468
  • Protein Accession : NP_461445
  • Taxonomy ID : 700720
  • Gene Starting Position : 2622804
  • Gene Ending Position : 2624381
  • Gene Strand (Orientation) : -
  • Protein Name : GMP synthase
  • Protein pI : 5.06
  • Protein Weight : 53458.79
  • Protein Length : 525
  • Protein Note : contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway
  • DNA Sequence : Show Sequence 
    >gi|16763390:2622804-2624381 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
CTTATTCCCACTCAATGGTAGCCGGCGGTTTACCGCTGATGTCATACACCACACGGGAAATCCCGTTGAC
TTCATTGATGATGCGGTTGGAAACGCGACCGAGGAAGTCATACGGCAGGTGCGCCCAGTGTGCGGTCATA
AAGTCGATGGTTTCGACGGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATAACGCCAA
CGGAACGAACCGGCAGGAAGACGGTAAACGCCTGACTGACTTTGTCGTACAGATCCGCTTTGCGCAGCTC
TTCAATGAAGATAGCGTCAGCACGGCGCAGCAGGTCGCAGTACTCTTTCTTCACTTCACCCAGAACACGA
ACGCCGAGGCCCGGCCCCGGGAACGGATGGCGATACAGCATGTCATACGGCAGGCCCAGCTCCAGACCAA
TCTTACGCACTTCGTCTTTGAACAGCTCTTTCAGCGGTTCAACCAGCCCCATCTTCATCTCTTTCGGCAA
GCCGCCGACATTGTGGTGAGATTTGATGACGTGCGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACG
TCAGGGTAAATGGTGCCTTGCGCCAGCCACTTCACGTCTTCCAGTTTGAGCGCTTCTTCGTCGAACACTT
CTACAAAAACACGACCGATGATCTTACGCTTGGCTTCCGGATCGTTCTCGCCAGCCAGCGCGGACAGGAA
GCGCTCTTCTGCCGGTACGTGAACGATATTCAGGCCAAAATGGTCGCCAAACATGTCCATCACCTGCTCG
GCTTCGTTGAGACGCAGCAGGCCGTTGTCGACGAATACACAGGTCAGATTTTTACCGATCGCGCGGTGCA
GCAGCATTGCGGTGACGGAAGAATCCACGCCACCAGAGAGGCCGAGGATCACTTTGTCGTCGCCCACCTG
CTCGCGAATGCGCGCCACGGCGTCGTCGATGATCTTCGCCGGCGTCCACAACGCTTCACACTGGCAGATA
TCGCGTACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTGTGAGTCACTTCCGGGTGGAACTGTACAC
CGTAGAAGCGTTTTTCTTCGTTGGCCATAATGGCGAACGGGCAGCTCTCGGTGCTGGCGACGGTCACGAA
GTCGGACGGAATCGCCGTCACTTTATCGCCGTGGCTCATCCACACGTCCAGCAGCGGTTTGCCGTCTGCG
GTCAGGGAATCTTCAATACCGCGAACCAGCGCGCTGTCGGTCAACACTTCGACCTGCGCATAACCAAATT
CACGCTCATTAGAACCTTCTACATGACCGCCAAGCTGCATCGCCATGGTCTGCATACCATAGCAAACGCC
AAATACCGGCACGCCTGCTTCAAAGACATACTGCAGCGCGCGCGGGCTGTTTTCTTCGGTGGTGCTTTCC
GGGCCGCCGGAAAGAATAATGCCGCTTGGGTTGAAGTCACGAATTTGTGCTTCTGTCACATCCCACGCCC
ACAGTTCACAGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTC
CAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCA
  • Protein Sequence : Show Sequence 
    >gi|16765830|ref|NP_461445.1| GMP synthase [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEAQIRDFNPSGIILSGGPESTTEENSPR
ALQYVFEAGVPVFGVCYGMQTMAMQLGGHVEGSNEREFGYAQVEVLTDSALVRGIEDSLTADGKPLLDVW
MSHGDKVTAIPSDFVTVASTESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTP
AKIIDDAVARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVMDMFGDH
FGLNIVHVPAEERFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQGTIYPDVIESAASATG
KAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELGLPYDMLYRHPFPGPGLGVRVLGEVKKEY
CDLLRRADAIFIEELRKADLYDKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPY
DFLGRVSNRIINEVNGISRVVYDISGKPPATIEWE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaBA and clpP mutant (CVD 1921) of Salmonella Typhimurium is attenuated in mice after oral immunization. The mutant was also protective, rendering a vaccine efficacy of 86% in mice against challenge with wild type Salmonella Typhimurium (Tennant et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium guaBA/clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
28. guaB
  • Gene Name : guaB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1254033
  • NCBI Protein GI : 16765831
  • Locus Tag : STM2511
  • Genbank Accession : AE006468
  • Protein Accession : NP_461446
  • Taxonomy ID : 700720
  • Gene Starting Position : 2624451
  • Gene Ending Position : 2625917
  • Gene Strand (Orientation) : -
  • Protein Name : inosine 5'-monophosphate dehydrogenase
  • Protein pI : 6.59
  • Protein Weight : 47186.87
  • Protein Length : 488
  • Protein Note : catalyzes the synthesis of xanthosine monophosphate by the NAD+ dependent oxidation of inosine monophosphate
  • DNA Sequence : Show Sequence 
    >gi|16763390:2624451-2625917 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
ATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTGGTGATGGTCACGTCGTGAACGTGGCTTTCCTGAAT
GCCCGCACCGCTGATACGCACAAACTCCGCTTTAGTACGCAGTTCGTCGATGGTAGCACAACCGGTCAGC
CCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGC
GACCTTCGATACCTTCCGGCACCAGTTTGTCAGCGGCGTTGTCGCTCTGGAAGTAACGGTCGGAGGAACC
TTTGGACATCGCACCCAGCGAGCCCATGCCGCGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATT
TCGCCCGGGGATTCTTCCGTACCCGCCAGCATGGAACCCACCATTACCGCGCTTGCACCTGCGGCAATCG
CTTTGGCTATGTCGCCGGAGAAACGGATACCGCCGTCAGCGATAACCGGAATACCGGTACCTTCCAGCGC
TTCAACTGCGTCAGAAACAGCGGTGATCTGCGGAACGCCCACGCCAGTCACGATACGAGTGGTACAGATG
GAACCCGGGCCAATACCGACTTTCACCGCGCTGCAACCGGCTTCCGCCAGTGCGCGAGCGCCTGCGCCTG
TCGCGACGTTGCCGCCGATGATTTGCAGGTCAGGATATTTAGCACGGGTTTCACGGATACGTTGCAACAC
GCCTTCTGAATGACCGTGAGAGGAGTCGATCAGCAGTACGTCAACGCCTGCCGCCACCAGCGCGTCAACG
CGCTCTTCGTTGCCCGCGCCTGCGCCGACCGCCGCGCCGACACGTAAACGGCCCTGCTCATCTTTGCAGG
AGTTTGGTTTACGTTCCGCTTTCTGGAAATCTTTTACGGTAATCATGCCAAGCAGATGGAAGTTATCATC
AACGACCAGCGCTTTTTCTACGCGTTTTTCGTGCATTTTTGCCAGCACGACTTCACGGGCTTCGCCTTCA
CGAACGGTCACCAGACGCTCTTTCGGCGTCATGTAAACGCTAACCGGCTGGTTCAGGTCAGTCACAAAAC
GCACGTCACGACCGGTGATGATACCCACCAGCTCGTTATCTTCAGTCACCACCGGATAGCCCGCAAAACC
GTTACGCTCGGTCAGGGCTTTCACTTCATGCAACGTGGTGGTTGGCAGGACGGTCTGCGGGTCGGTCACT
ACGCCGGACTCGTGTTTCTTCACGCGGCGAACTTCTTCCGCCTGGCGCTCAATGGACATGTTTTTGTGGA
TAAAACCAATGCCGCCTTCCTGGGCCAGGGCAATTGCCAGGCGCGCTTCCGTCACGGTGTCCATCGCCGC
AGAAAGCATAGGAATATTCAGACGAATAGTTTTCGTCAACTGCGTGCTGAGATCGGCAGTATTCGGCAAA
ACGGTGGAGTGAGCGGGAACAAGGAGGACGTCGTCAAACGTCAGGGCTTCTTTAGCGATACGTAGCA
  • Protein Sequence : Show Sequence 
    >gi|16765831|ref|NP_461446.1| inosine 5'-monophosphate dehydrogenase [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARLAIALAQEGGIGFI
HKNMSIERQAEEVRRVKKHESGVVTDPQTVLPTTTLHEVKALTERNGFAGYPVVTEDNELVGIITGRDVR
FVTDLNQPVSVYMTPKERLVTVREGEAREVVLAKMHEKRVEKALVVDDNFHLLGMITVKDFQKAERKPNS
CKDEQGRLRVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVAT
GAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVSDAVEALEGTGIPVIADGGIRFSGDIAKA
IAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLGAMSKGSSDRYFQSDNAADKLVPEGIEGR
VAYKGRLKEIIHQQMGGLRSCMGLTGCATIDELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaBA and clpP mutant (CVD 1921) of Salmonella Typhimurium is attenuated in mice after oral immunization. The mutant was also protective, rendering a vaccine efficacy of 86% in mice against challenge with wild type Salmonella Typhimurium (Tennant et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium guaBA/clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
29. guaB from S. enteritidis str. P125109
  • Gene Name : guaB from S. enteritidis str. P125109
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Enteritidis str. P125109
  • NCBI Gene ID : 6949287
  • NCBI Protein GI : 207857921
  • Locus Tag : SEN2491
  • Genbank Accession : AM933172
  • Protein Accession : YP_002244572
  • Taxonomy ID : 550537
  • Gene Starting Position : 2625484
  • Gene Ending Position : 2626950
  • Gene Strand (Orientation) : -
  • Protein Name : inosine 5'-monophosphate dehydrogenase
  • Protein pI : 6.59
  • Protein Weight : 47186.87
  • Protein Length : 488
  • Protein Note : catalyzes the synthesis of xanthosine monophosphate by the NAD+ dependent oxidation of inosine monophosphate
  • DNA Sequence : Show Sequence 
    >gi|207855516:2625484-2626950 Salmonella enterica subsp. enterica serovar Enteritidis str. P125109 chromosome, complete genome
ATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTGGTGATGGTCACGTCGTGAACGTGGCTTTCCTGGAT
ACCCGCACCGCTGATACGCACAAACTCCGCTTTAGTACGCAGTTCGTCGATGGTAGCACAACCGGTCAGC
CCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGC
GGCCTTCGATACCTTCCGGCACCAGTTTGTCGGCGGCGTTGTCGCTCTGGAAGTAACGGTCGGAGGAACC
TTTGGACATCGCGCCCAGAGAACCCATACCGCGATAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATT
TCGCCCGGGGATTCTTCGGTACCGGCCAGCATAGAACCCACCATTACCGCGCTCGCGCCTGCGGCGATGG
CTTTGGCGATGTCGCCGGAGAAACGGATACCGCCGTCAGCGATAACCGGAATCCCGGTGCCTTCCAGCGC
TTCCACCGCGTCAGAAACAGCGGTGATCTGCGGAACGCCCACACCAGTCACGATACGAGTCGTACAGATG
GAACCCGGGCCGATACCCACTTTCACCGCGCTGCAACCGGCTTCCGCCAGTGCGCGAGCGCCTGCGCCCG
TCGCAACGTTGCCGCCGATGATTTGCAGGTCAGGATATTTAGCACGCGTCTCACGGATACGTTGCAACAC
GCCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGTACGTCAACGCCTGCCGCCACCAGCGCGTCAACG
CGCTCTTCGTTGCCCGCGCCTGCGCCGACCGCCGCGCCGACACGTAAACGGCCCTGCTCATCTTTACAGG
AGTTTGGTTTACGTTCCGCTTTCTGGAAATCTTTTACGGTAATCATGCCAAGCAGATGGAAGTTATCATC
AACGACCAGCGCTTTTTCTACGCGTTTTTCGTGCATTTTTGCCAGCACGACTTCACGGGCTTCGCCTTCA
CGAACGGTCACCAGACGCTCTTTCGGTGTCATGTAGACACTCACCGGCTGGTTCAGGTCAGTCACAAAAC
GCACGTCACGACCGGTGATGATACCCACCAGCTCGTTATCTTCAGTCACCACCGGATAGCCCGCAAAACC
GTTACGCTCGGTCAGGGCTTTCACTTCATGCAACGTGGTGGTTGGCAGGACGGTCTGCGGGTCGGTCACT
ACGCCGGACTCGTGTTTCTTCACGCGGCGAACTTCTTCCGCCTGGCGCTCAATAGACATGTTTTTGTGGA
TAAAACCAATGCCGCCTTCCTGGGCCAGGGCAATTGCCAGGCGCGCTTCCGTCACGGTGTCCATCGCCGC
AGAAAGCATAGGAATATTCAGACGAATAGTTTTCGTCAACTGCGTGCTGAGATCGGCAGTATTCGGCAAA
ACGGTGGAGTGAGCGGGAACAAGGAGGACGTCGTCAAACGTCAGAGCTTCTTTAGCGATACGTAGCA
  • Protein Sequence : Show Sequence 
    >gi|207857921|ref|YP_002244572.1| inosine 5'-monophosphate dehydrogenase [Salmonella enterica subsp. enterica serovar Enteritidis str. P125109]
MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARLAIALAQEGGIGFI
HKNMSIERQAEEVRRVKKHESGVVTDPQTVLPTTTLHEVKALTERNGFAGYPVVTEDNELVGIITGRDVR
FVTDLNQPVSVYMTPKERLVTVREGEAREVVLAKMHEKRVEKALVVDDNFHLLGMITVKDFQKAERKPNS
CKDEQGRLRVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVAT
GAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVSDAVEALEGTGIPVIADGGIRFSGDIAKA
IAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLGAMSKGSSDRYFQSDNAADKLVPEGIEGR
VAYKGRLKEIIHQQMGGLRSCMGLTGCATIDELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A guaB mutant, in combination with a fliC mutant, is attenuated in mice and chickens and induced significant protection from challenge with wild type S. enterica (Adriaensen et al., 2007).
  • Related Vaccine(s): Salmonella enterica fliC/guaB mutant vaccine , Salmonella Enteritidis guaBA/clpP mutant vaccine
30. gyrA
  • Gene Name : gyrA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253794
  • NCBI Protein GI : 16765599
  • Locus Tag : STM2272
  • Genbank Accession : AE006468
  • Protein Accession : NP_461214
  • Taxonomy ID : 700720
  • Gene Starting Position : 2373710
  • Gene Ending Position : 2376346
  • Gene Strand (Orientation) : -
  • Protein Name : DNA gyrase subunit A
  • Protein pI : 4.73
  • Protein Weight : 89524.22
  • Protein Length : 878
  • Protein Note : negatively supercoils closed circular double-stranded DNA
  • DNA Sequence : Show Sequence 
    >gi|16763390:2373710-2376346 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
ATTACTCGTCAGCGTCATCCGCAACGTCGTCATCGCTTTCCGCTTCCGGGGCGATATCCTCATCCCCTTC
CGCCACGCTGCCGTCGATAGCGTCGAGTTCTTCGTCATCTACCGGTTCAGCAACGCGTTGCAGACCCACC
ACGTTTTCATCTTCCGCCGTGCGGATAAGGATAACGCCCTGGGTATTACGTCCCACTACGCTGATCTCGG
ACACACGGGTACGCACCAGAGTACCGGCATCCGTGATCATCATGATCTGGTCGCAATCGTCTACCTGTAC
CGCACCGACAACGGAACCGTTGCGCTCGGTCACTTTGATAGAGATAACGCCCTGCGTCGCACGAGACTTG
GTCGGGTACTCGTCCGCTGCGGTACGCTTCCCGTAGCCGTTTTGCGTTACGGTCAGAATAGCGCCTTCGC
CGCGTGGGATGATCAGAGAGACGACTTTATCGTCTCCCGCCAGCTTAATACCGCGCACACCGGTCGCGGT
ACGCCCCATCGCACGGACGGCGTCTTCTTTGAAGCGCACCACTTTACCCGCGGCCGAGAACAGCATGACT
TCGTCAGAACCAGAAGTCAGGTCAACGCCAATCAGCTCGTCGCCGTCGTTGAGGTTCACCGCGATAATAC
CGGCGGAACGCGGACGGCTGAATTCGGTCAGCGCCGTTTTCTTCACGGTACCGCTGGCGGTCGCCATAAA
GACGTTGACGCCTTCTTCATACTCACGAACCGGCAGAATCGCGGTGATACGTTCGTTGGCTTCCAGCGGC
AGCAGGTTGACGATCGGACGACCGCGCGCGCCGCGGCTGGCTTCCGGCAGCTGATAGACCTTCATCCAGT
ACAGACGGCCCCGGCTGGAGAAGCAGAGGATGGTGTCATGGGTGTTAGCCACCAGCAGGCGGTCGATAAA
GTCTTCTTCTTTAATACGCGCGGCAGATTTACCTTTCCCACCACGACGTTGCGCTTCGTAATCTGTCAGC
GGTTGATATTTGACGTAACCCTGGTGAGACAGCGTCACGACAACATCTTCCTGGCTAATCAGATCTTCGA
TATTAATATCGGCGCTGTTGGCGGTGATTTCGGTACGACGCTCATCGCCGAACTGATCGCGAATTAACTC
CATCTCTTCGCGGATCACTTCCATCAGGCGATCGGCGCTGCCCAGAATGTGCAGCAATTCAGCAATCTGC
TCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCATGCTCCAGGCCGGTCAGTTTCTGCAAACGCAGAT
CCAGAATCGCCTGCGCCTGCTGTTCAGTCAGGTAGTACTGACCGTCACGCACGCCAAATTCTGGCTCCAG
CCATTCCGGACGCGCGGCGTCATCACCAGCGCGCTCCAGCATCGCAGCAACGTTGCCCAGATCCCACGGA
CGCGAAATCAGCGCCGCTTTTGCTTCCGCCGGCGTTGGCGCGCGGCGAATCAGTTCGATAATCGGGTCGA
TGTTGGCCAGCGCAATCGCCAGAGCTTCAAGGATATGCGCACGGTCACGGGCTTTACGCAGTTCAAAAAT
AGTCCGACGCGTCACCACTTCACGGCGGTGGCGCACGAACGCTGAAATGATATCTTTCAGGTTCATGATC
TTCGGCTGGCCGTGATGCAGCGCCACCATGTTAATACCGAAGGAAACCTGTAGCTGGGTCTGGGAGTAGA
GATTATTAAGCACCACCTCGCCCACCGCATCGCGTTTCACTTCAATCACGATGCGCATCCCGTCTTTGTC
GGATTCGTCACGCAGCGCGCTGATGCCTTCCACGCGTTTATCTTTCACCAGCTCGGCGATTTTCTCGATC
AGGCGCGCTTTGTTCACCTGATAGGGAATTTCATGGACGATGATGGTTTCACGGCCCGTTTTGGCGTCAG
CTTCAACTTCCGCGCGGGCGCGAATGTACACTTTGCCACGACCGGTGCGGTAGGCTTCTTCGATACCACG
ACGACCGTTGATGATCGCGGCGGTCGGGAAGTCCGGCCCCGGAATATGTTCCATCAGCCCTTCAATGCTG
ATGTCTTCGTTGTCGATATACGCCAGGCAGCCGTTAATCACTTCCGTCAGGTTGTGCGGCGGGATATTCG
TCGCCATACCTACTGCGATACCGGAAGAACCGTTCACCAGCAGATTCGGAATTTTGGTCGGCATGACGTC
CGGAATTTTTTCCGTACCGTCATAGTTATCCACGAAATCCACCGTCTCTTTTTCGAGATCGGCCATCAGT
TCGTGGGCGATTTTCGCCAGACGGATCTCCGTATAACGCATTGCCGCCGCGGAGTCGCCGTCAATAGAAC
CGAAGTTACCCTGACCATCCACCAGCATGTAACGCAGCGAGAATGGCTGCGCCATACGAACGATGGTGTC
ATACACTGCGGAATCGCCGTGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTA
TAGGCTTTGTTCCAGTCATTGCCCAATACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGC
CATCTCGGACATCCGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAG
CTCCTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCA
  • Protein Sequence : Show Sequence 
    >gi|16765599|ref|NP_461214.1| DNA gyrase subunit A [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLYAMNVLGNDWNKAYKKSARVV
GDVIGKYHPHGDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEK
ETVDFVDNYDGTEKIPDVMPTKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDNEDISIEGLME
HIPGPDFPTAAIINGRRGIEEAYRTGRGKVYIRARAEVEADAKTGRETIIVHEIPYQVNKARLIEKIAEL
VKDKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINMVALHHGQPKIMNLKDI
ISAFVRHRREVVTRRTIFELRKARDRAHILEALAIALANIDPIIELIRRAPTPAEAKAALISRPWDLGNV
AAMLERAGDDAARPEWLEPEFGVRDGQYYLTEQQAQAILDLRLQKLTGLEHEKLLDEYKELLEQIAELLH
ILGSADRLMEVIREEMELIRDQFGDERRTEITANSADINIEDLISQEDVVVTLSHQGYVKYQPLTDYEAQ
RRGGKGKSAARIKEEDFIDRLLVANTHDTILCFSSRGRLYWMKVYQLPEASRGARGRPIVNLLPLEANER
ITAILPVREYEEGVNVFMATASGTVKKTALTEFSRPRSAGIIAVNLNDGDELIGVDLTSGSDEVMLFSAA
GKVVRFKEDAVRAMGRTATGVRGIKLAGDDKVVSLIIPRGEGAILTVTQNGYGKRTAADEYPTKSRATQG
VISIKVTERNGSVVGAVQVDDCDQIMMITDAGTLVRTRVSEISVVGRNTQGVILIRTAEDENVVGLQRVA
EPVDDEELDAIDGSVAEGDEDIAPEAESDDDVADDADE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A gyrA-cpxA-rpoB Salmonella Typhimurium mutant was attenuated and protected against challenge in pigs (Roesler et al., 2004).
  • Related Vaccine(s): Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
31. hfq
  • Gene Name : hfq
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1255887
  • NCBI Protein GI : 16767607
  • Locus Tag : STM4361
  • Genbank Accession : AE006468
  • Protein Accession : NP_463222
  • 3D structure: PDB ID : 2YLB
  • Taxonomy ID : 700720
  • Gene Starting Position : 4604574
  • Gene Ending Position : 4604882
  • Gene Strand (Orientation) : +
  • Protein Name : RNA-binding protein Hfq
  • Protein pI : 7.9
  • Protein Weight : 10240.49
  • Protein Length : 102
  • Protein Note : Stimulates the elongation of poly(A) tails
  • DNA Sequence : Show Sequence 
    >gi|16763390:4604574-4604882 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
AATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCATTGCGTCGGGAACGTGTTCCAGTTTCT
ATTTATTTGGTGAATGGTATTAAGCTGCAAGGTCAAATCGAGTCCTTTGATCAGTTCGTGATCCTGTTGA
AGAACACGGTCAGCCAGATGGTTTATAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCCCA
TCACAGCAACAATGCCGGTGGCGGCGCCAGCAATAACTACCATCACGGTAGCAACGCGCAGGGGTCTACT
GCGCAACAGGACAGCGAAGAGACTGAATA
  • Protein Sequence : Show Sequence 
    >gi|16767607|ref|NP_463222.1| RNA-binding protein Hfq [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVILLKNTVSQMVYKHAISTVVPSRPVSH
HSNNAGGGASNNYHHGSNAQGSTAQQDSEETE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Oral immunization with the Salmonella hfq deletion mutant efficiently protects mice against subsequent oral challenge with virulent strain of Salmonella Typhimurium (Allam et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium hfq mutant vaccine
32. hslU
  • Gene Name : hslU
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • VO ID : VO_0011266
  • NCBI Protein GI : 9650897
  • Other Database IDs : CDD:235364
    GOA:O30911
    InterPro: IPR003593
    InterPro: IPR004491
    InterPro: IPR013093
    UniProtKB/UniProt: O30911
  • Taxonomy ID : 90371
  • Protein Name : heat shock protein
  • Protein pI : 5.22
  • Protein Weight : 47057.28
  • Protein Length : 540
  • Protein Note : ATP-dependent protease ATP-binding subunit HslU; Provisional; PRK05201
  • Protein Sequence : Show Sequence 
    >CAC00720.1 heat shock protein [Salmonella enterica subsp. enterica serovar Typhimurium]
MSEMTPREIVSELNKHIIGQDNAKRSVAIALRNRWRRMQLDEELRHEVTPKNILMIGPTGVGKTEIARRL
AKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAK
NNWGQAEQQQEPSAARQTFRKKLREGQLDDKEIEINLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQK
QKPRKLKIKDAMKLLVEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGETSGPDVSREGVQRD
LLPLVEGCTVSTKHGMVKTDHILFIASGAFQVAKPSDLIPELQGRLPIRVELQALTTSDFERILTEPNAS
VTVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNETTENIGARRLHTVLERLMEEISYNASDLHGQNITI
DAEYVSKHLDALVADEDLSRFIL
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Study demonstrated that a recombinant construction of heat-shock protein (hslU) with lypopolysaccharide (rcHSP-LPS) administered 24 hours before inoculation induced resistance to S. typhimurium infection. Protection formed after 3 injections of rcHSP-LPS with 10 mcg in each or single injection with 100 mcg/mouse. Forty to eighty percent of immunized mice survived after challenge while 90% of control animals died. Destruction of the HSP by boiling of the construction led to loss of protective effect (Vorob'ev et al., 2009).
33. HtrA
  • Gene Name : HtrA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. CT18
  • NCBI Gene ID : 1246721
  • NCBI Protein GI : 16759200
  • Locus Tag : STY0231
  • Genbank Accession : NC_003198.1
  • Protein Accession : NP_454817.1
  • Taxonomy ID : 28901
  • Gene Starting Position : 241500
  • Gene Ending Position : 242927
  • Gene Strand (Orientation) : +
  • Protein Name : protease DO precursor; heat shock protein HtrA
  • Protein pI : 9.27
  • Protein Weight : 49287.9
  • Protein Length : 475
  • Protein Note : Fasta hit to DEGQ_ECOLI (455 aa), 57% identity in 478 aa overlap Orthologue of E. coli htrA (HTRA_ECOLI); Fasta hit to HTRA_ECOLI (474 aa), 92% identity in 475 aa overlap
  • DNA Sequence : Show Sequence 
    >GeneID|1246721 [Salmonella enterica subsp. enterica serovar Typhi str. CT18 ] 241500..242927
atgaaaaaaaccacattagcaatgagtgcactggctctgagtttaggtttggcattgtcgcctctgtctg
ccacggcggctgaaacgtcctcttcagcaatgactgcccagcagatgccaagcctggcaccgatgctcga
aaaagtgatgccatcggtggtcagtattaatgtagaaggtagcaccacggtgaatacgccgcgtatgccg
cgtaatttccagcaattctttggcgatgactccccgttctgccaggacggttctccgttccagaattctc
cgttctgccagggcggcggtaacggcggcaacggcggtcaacaacagaaattcatggcgctgggctccgg
cgtaattattgacgccgcgaagggctacgtcgtcaccaacaaccacgtggttgataacgccagcgtgatt
aaagtacagcttagcgatgggcgtaaattcgatgctaaagtggtgggcaaagatccgcgttctgatatcg
cgctgattcaaattcagaatccgaagaacctgacggcgattaagctggcggactccgacgcgctgcgcgt
gggggattataccgtcgctattggtaacccgtttggtctgggcgaaacggtgacgtcaggtatcgtttcg
gcgctggggcgtagcggcctgaacgtagaaaattacgagaactttattcagaccgacgccgcgattaacc
gcggtaactccggcggcgcgctggtgaacctgaacggtgagctgatcggtattaacaccgcgattctggc
gccggacggcggcaacatcggtatcggcttcgctatccccagtaacatggtgaaaaacctgacgtcgcag
atggtggaatacggccaggtgaaacgcggcgaactggggatcatggggactgagctgagctctgagctgg
cgaaagcgatgaaagtcgacgcccagcgtggcgcgttcgtcagccaggtgatgccgaattcgtccgcagc
gaaagcgggtatcaaagccggggatgtcattacctcgctgaacggtaaaccgatcagcagctttgcggcg
ctgcgcgctcaggtcggcactatgccggtcggcagcaaaatcagcctcggtctgctgcgtgaaggtaaag
cgattacggttaatctggaactgcagcagagcagccagagtcaggttgattccagcaccatcttcagcgg
gattgagggcgctgaaatgagtaataaaggccaggataaaggcgttgtggtgagcagcgtgaaagcgaac
tcacccgccgcgcaaattggcctcaaaaaaggcgatgtgattatcggcgctaaccagcagccggtgaaaa
atatcgccgagctgcgtaagattctcgacagcaagccgtcggtgctggcgctgaatattcagcgtggtga
tagttctatttatttgctgatgcagtaa
  • Protein Sequence : Show Sequence 
    >gi|16759200|ref|NP_454817.1| protease DO precursor; heat shock protein HtrA [Salmonella enterica subsp. enterica serovar Typhi str. CT18 ]
MKKTTLAMSALALSLGLALSPLSATAAETSSSAMTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMP
RNFQQFFGDDSPFCQDGSPFQNSPFCQGGGNGGNGGQQQKFMALGSGVIIDAAKGYVVTNNHVVDNASVI
KVQLSDGRKFDAKVVGKDPRSDIALIQIQNPKNLTAIKLADSDALRVGDYTVAIGNPFGLGETVTSGIVS
ALGRSGLNVENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQ
MVEYGQVKRGELGIMGTELSSELAKAMKVDAQRGAFVSQVMPNSSAAKAGIKAGDVITSLNGKPISSFAA
LRAQVGTMPVGSKISLGLLREGKAITVNLELQQSSQSQVDSSTIFSGIEGAEMSNKGQDKGVVVSSVKAN
SPAAQIGLKKGDVIIGANQQPVKNIAELRKILDSKPSVLALNIQRGDSSIYLLMQ
  • Related Vaccine(s): CVD 909
34. htrA
  • Gene Name : htrA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium SL1344
  • NCBI Protein GI : 301156836
  • Locus Tag : STM0209
  • Genbank Accession : AE006468
  • Protein Accession : NP_459214
  • Other Database IDs : CDD:182855
    CDD:199257
    CDD:29044
    UniProtKB/TrEMBL: E1W881
  • Taxonomy ID : 216597
  • Gene Starting Position : 244491
  • Gene Ending Position : 245918
  • Gene Strand (Orientation) : +
  • Protein Name : protease DO precursor; heat shock protein HtrA
  • Protein pI : 9.27
  • Protein Weight : 45544.93
  • Protein Length : 475
  • Protein Note : serine endoprotease; Provisional
  • DNA Sequence : Show Sequence 
    >gi|16763390:244491-245918 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
CATGAAAAAAACCACATTAGCAATGAGTGCACTGGCTCTGAGTTTAGGTTTGGCATTGTCGCCTCTGTCT
GCCACGGCGGCTGAAACGTCCTCTTCAGCAATGACTGCCCAGCAGATGCCAAGCCTGGCACCGATGCTCG
AAAAAGTGATGCCATCGGTGGTCAGTATTAATGTTGAAGGTAGCACCACGGTGAATACGCCGCGTATGCC
GCGTAATTTCCAGCAGTTCTTTGGCGATGACTCCCCGTTCTGCCAGGACGGTTCTCCGTTCCAGAATTCT
CCGTTCTGCCAGGGCGGCGGTAACGGCGGCAACGGCGGTCAACAACAGAAATTCATGGCGCTGGGCTCCG
GCGTAATTATTGACGCCGCGAAGGGCTACGTCGTCACCAACAACCACGTGGTTGATAACGCCAGCGTGAT
TAAAGTACAGCTTAGCGATGGGCGTAAATTCGATGCTAAAGTGGTGGGCAAAGATCCGCGTTCTGATATC
GCGCTGATTCAAATTCAGAATCCGAAGAACCTGACGGCGATTAAGCTGGCGGACTCCGACGCGCTGCGCG
TGGGGGATTATACCGTCGCTATTGGTAACCCGTTTGGTCTGGGCGAAACGGTGACGTCAGGTATCGTTTC
GGCGCTGGGGCGTAGCGGCCTGAACGTAGAAAATTACGAGAACTTTATTCAGACCGACGCCGCGATTAAC
CGTGGTAACTCCGGCGGCGCGCTGGTGAACCTGAACGGTGAGCTGATCGGTATTAACACCGCGATTCTGG
CGCCGGACGGCGGCAACATCGGTATCGGCTTCGCTATCCCCAGTAACATGGTGAAAAACCTGACGTCGCA
GATGGTGGAATACGGCCAGGTGAAACGCGGCGAACTGGGGATCATGGGGACTGAGCTGAATTCCGAATTG
GCGAAAGCGATGAAAGTCGACGCCCAGCGAGGCGCGTTCGTCAGCCAGGTGATGCCGAATTCGTCCGCGG
CGAAAGCGGGTATCAAAGCCGGGGATGTCATTACCTCGCTGAACGGTAAACCGATCAGCAGCTTTGCGGC
GCTGCGCGCTCAGGTCGGCACTATGCCGGTCGGCAGCAAAATCAGCCTCGGTCTGCTGCGTGAAGGTAAA
GCGATTACGGTGAATCTGGAACTGCAGCAGAGCAGCCAGAGTCAGGTTGATTCCAGCACCATCTTCAGCG
GGATTGAAGGCGCTGAAATGAGCAATAAAGGCCAGGATAAAGGCGTTGTGGTGAGCAGCGTGAAAGCGAA
CTCACCCGCCGCGCAAATTGGCCTCAAAAAAGGCGATGTGATTATCGGCGCTAACCAGCAGCCGGTGAAA
AATATCGCCGAGCTGCGTAAGATTCTCGACAGCAAGCCGTCGGTTCTGGCGCTGAATATTCAGCGTGGTG
ATAGTTCTATTTATTTGCTGATGCAGTA
  • Protein Sequence : Show Sequence 
    >gi|301156836|emb|CBW16312.1| protease DO precursor; heat shock protein HtrA [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
MKKTTLAMSALALSLGLALSPLSATAAETSSSAMTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMP
RNFQQFFGDDSPFCQDGSPFQNSPFCQGGGNGGNGGQQQKFMALGSGVIIDAAKGYVVTNNHVVDNASVI
KVQLSDGRKFDAKVVGKDPRSDIALIQIQNPKNLTAIKLADSDALRVGDYTVAIGNPFGLGETVTSGIVS
ALGRSGLNVENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQ
MVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVMPNSSAAKAGIKAGDVITSLNGKPISSFAA
LRAQVGTMPVGSKISLGLLREGKAITVNLELQQSSQSQVDSSTIFSGIEGAEMSNKGQDKGVVVSSVKAN
SPAAQIGLKKGDVIIGANQQPVKNIAELRKILDSKPSVLALNIQRGDSSIYLLMQ
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A htrA mutant in Salmonella typhimurium is attenuated and provides protection in mice against an oral challenge with virulent S. typhimurium SL1344 (Chatfield et al., 1992).
  • Related Vaccine(s): Salmonella Typhimurium htrA mutant vaccine
35. IroN
  • Gene Name : IroN
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • VO ID : VO_0010997
  • NCBI Protein GI : 259475459
  • Other Database IDs : CDD:237410
  • Taxonomy ID : 90371
  • Gene Strand (Orientation) : ?
  • Protein pI : 6.28
  • Protein Weight : 79296.4
  • Protein Length : 857
  • Protein Note : outer membrane receptor FepA; Provisional
  • Protein Sequence : Show Sequence 
    >CBF90638.1 unnamed protein product [Salmonella enterica subsp. enterica serovar Typhimurium]
MNKKIHSLTLLVNLGIYGATLPVMAEDKTDSAALTNEDTIVVTAAQQNLQAPGVSTITADEIRKNPPARD
VSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPENTLILIDGKPVTSRNSVRLGWRGERDTRGDTAWV
PPEMIERIEVLRGPAAARYGNGAAGGVVNIITKKGGSEWHGSWNTYFNAPEHKDEGATKRTNFSLNGPLG
GDFSFRLYGNLDKTQADARNINQGHQSERTGSYADTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
RQGNLYAGDTQNTNTNQLVKDNYGKETNRLYRQNYSLTWNGGWNNGVTTSNWVQYEHTRNSRMPEGLAGG
TEGIFDPKASQKYADADLNDVTLHSEVSLPFDLLVNQNLTLGTEWAQQRMKDQLSNSQTFMGGNIPGYSS
TNRSPYSKAEIFSLFAENNMELTDSTMLTPGLRFDHHSIVGDNWSPSLNLSQGLGDDFTLKMGIARAYKA
PSLYQTNPNYILYSKGQGCYATGAGTGIGCYMMGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRN
KIEAGTVPLQRINNGKTDVYQWENVPKAVVEGLEGTLNVPVSDTVNWTNNVTYMLQSKNKETGERLSIIP
QYTLNSTLSWQVRQDVSLQSTFTWYGKQEPKKYDYQGNPVTGTDKQAVSPYSIVGLSATWDVTKNVSLTG
GVDNLFDKRLWREGNAQTVRDTQTGAYMAGAGAYTYNEPGRTWYMSINTHF
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Thirty-day-old chickens were immunized intramuscularly with purified IroN from Salmonella Typhimurium mixed with Freund's incomplete adjuvant; the chickens were then challenged intravenously with Salmonella Enteritidis. The mortality rate of immunized chickens was 10%. On the other hand, that of control chickens was 80% (Kaneshige et al., 2009).
  • Related Vaccine(s): Salmonella IroN Protein Vaccine
36. lon
  • Gene Name : lon
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6923289
  • NCBI Protein GI : 205351761
  • Locus Tag : SG0461
  • Genbank Accession : AM933173
  • Protein Accession : YP_002225562
  • Taxonomy ID : 550538
  • Gene Starting Position : 503376
  • Gene Ending Position : 505730
  • Gene Strand (Orientation) : ?
  • Protein Name : DNA-binding ATP-dependent protease La
  • Protein pI : 6.32
  • Protein Weight : 81259.62
  • Protein Length : 784
  • DNA Sequence : Show Sequence 
    >gi|205351346:503376-505730 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
TATGAATCCTGAGCGTTCTGAACGCATTGAAATCCCCGTATTGCCCTTGCGCGATGTGGTGGTTTATCCG
CACATGGTCATACCCTTATTTGTAGGGCGGGAGAAATCTATCCGTTGTCTGGAAGCGGCTATGGACCATG
ATAAAAAAATCATGCTGGTCGCCCAGAAGGAAGCATCGACGGATGAGCCGGGTGTAAACGATCTTTTCAC
CGTCGGGACCGTGGCCTCTATTTTGCAGATGCTGAAACTGCCTGACGGCACCGTAAAAGTGCTGGTCGAG
GGGTTACAGCGCGCGCGTATTTCCGCGCTGTCTGACAACGGCGAACATTTCTCCGCGAAGGCGGAGTACC
TTGATTCGCCAGCCATTGATGAGCGTGAACAAGAAGTGCTGGTGCGTACCGCTATCAGCCAGTTCGAAGG
CTACATCAAGCTGAACAAAAAAATTCCGCCGGAAGTGCTGACGTCACTCAATAGCATTGACGACCCTGCA
CGTCTGGCTGACACCATCGCCGCGCATATGCCGCTGAAACTGGCGGATAAACAGTCCGTACTGGAGATGT
CCGACGTTAACGAACGTCTGGAATATCTGATGGCGATGATGGAATCTGAAATCGATCTGCTGCAGGTTGA
GAAACGCATTCGCAACCGCGTGAAAAAGCAGATGGAGAAATCCCAGCGCGAGTACTATCTGAACGAGCAA
ATGAAAGCGATTCAGAAAGAGCTTGGAGAGATGGACGACGCGCCGGACGAAAACGAAGCGCTGAAGCGTA
AGATCGACGCGGCGAAAATGCCGAAAGAGGCGAAAGAGAAAGCGGAAGCGGAACTGCAGAAGCTGAAAAT
GATGTCTCCGATGTCGGCGGAAGCGACCGTGGTGCGCGGCTACATCGACTGGATGGTGCAGGTGCCGTGG
AATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTCAGGCGCAGGAAATCCTCGATACTGACCATTATGGTC
TGGAGCGCGTGAAAGACCGCATCCTTGAGTATCTCGCGGTTCAAAGCCGTGTAAATAAAATCAAGGGGCC
GATCCTGTGTCTGGTGGGGCCGCCGGGGGTAGGTAAAACCTCGTTGGGCCAGTCCATCGCTAAAGCAACA
GGGCGTAAATACATCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGCGGTCATCGCCGGA
CTTACATTGGCTCTATGCCGGGTAAACTGATCCAAAAAATGGCCAAAGTGGGGGTTAAAAACCCACTGTT
CCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGCGGCGATCCGGCTTCCGCACTGCTGGAAGTG
CTGGATCCGGAGCAGAACGTGGCGTTCAGCGATCATTACCTGGAAGTGGATTACGATCTCAGCGATGTGA
TGTTCGTCGCCACTTCCAACTCCATGAACATTCCGGCGCCGCTGCTGGATCGTATGGAAGTGATCCGTCT
GTCCGGTTATACCGAAGACGAGAAGCTGAATATTGCTAAACGTCACCTGCTGCCGAAGCAGATCGAGCGT
AACGCGCTGAAGAAAGGCGAACTGACGGTCGACGATAGCGCGATTATCGGTATTATCCGCTATTACACGC
GTGAAGCGGGCGTTCGTAGTCTGGAGCGTGAAATCTCCAAACTGTGCCGCAAAGCGGTTAAACAACTGTT
GCTCGATAAGTCGTTAAAACATATCGAAATTAACGGTGATAATCTGCACGACTACCTCGGTGTGCAGCGC
TTTGACTATGGCCGCGCGGATAGCGAAAACCGCGTAGGTCAGGTGACGGGCCTGGCGTGGACGGAAGTGG
GCGGCGATCTGTTGACCATCGAAACCGCCTGCGTCCCGGGCAAAGGCAAGTTGACCTACACCGGCTCGCT
CGGTGAAGTGATGCAGGAATCCATTCAGGCGGCGCTGACCGTGGTGCGTGCGCGCGCGGAAAAACTGGGT
ATTAATCCGGACTTTTACGAAAAACGCGATATCCACGTTCACGTGCCGGAAGGCGCAACGCCGAAAGACG
GTCCAAGCGCCGGTATCGCGATGTGTACCGCGCTGGTTTCCTGCCTGACCGGTAATCCGGTGCGCGCAGA
TGTGGCAATGACCGGTGAGATCACGCTGCGTGGTCAGGTGTTGCCGATCGGCGGCCTGAAAGAAAAACTG
TTGGCGGCGCATCGCGGCGGGATTAAAACGGTCTTAATTCCGTTCGAAAATAAACGTGACCTGGAGGAAA
TTCCGGACAACGTGATTGCCGATCTGGATATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACGCTTGC
TCTGCAAAACGAACCGTCTGGAATGCAGGTTGTAACCGCAAAATA
  • Protein Sequence : Show Sequence 
    >gi|205351761|ref|YP_002225562.1| DNA-binding ATP-dependent protease La [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAMDHDKKIMLVAQKEASTDEPGVNDLFT
VGTVASILQMLKLPDGTVKVLVEGLQRARISALSDNGEHFSAKAEYLDSPAIDEREQEVLVRTAISQFEG
YIKLNKKIPPEVLTSLNSIDDPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVE
KRIRNRVKKQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQKLKM
MSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRILEYLAVQSRVNKIKGP
ILCLVGPPGVGKTSLGQSIAKATGRKYIRMALGGVRDEAEIRGHRRTYIGSMPGKLIQKMAKVGVKNPLF
LLDEIDKMSSDMRGDPASALLEVLDPEQNVAFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRL
SGYTEDEKLNIAKRHLLPKQIERNALKKGELTVDDSAIIGIIRYYTREAGVRSLEREISKLCRKAVKQLL
LDKSLKHIEINGDNLHDYLGVQRFDYGRADSENRVGQVTGLAWTEVGGDLLTIETACVPGKGKLTYTGSL
GEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGIAMCTALVSCLTGNPVRAD
VAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFENKRDLEEIPDNVIADLDIHPVKRIEEVLTLA
LQNEPSGMQVVTAK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A lon mutant, in combination with a cpxR mutation, is highly attenuated in mice and induces significant protection from challenge with wild type S. gallinarum (Matsuda et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum cpxR/Ion mutant vaccine
37. lppA
  • Gene Name : lppA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • NCBI Protein GI : 323129647
  • Other Database IDs : CDD:187093
  • Taxonomy ID : 909946
  • Gene Strand (Orientation) : ?
  • Protein Name : major outer membrane lipoprotein
  • Protein Length : 78
  • Protein Note : Murein lipoprotein [Cell envelope biogenesis, outer membrane]; cl11539
  • Protein Sequence : Show Sequence 
    >gi|323129647|gb|ADX17077.1| major outer membrane lipoprotein [Salmonella enterica subsp. enterica serovar Typhimurium str. 4/74]
MNRTKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQLSNDVNAMRSDVQAAKDDAARANQRLD
NQATKYRK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An lppA mutant, in combination with an lppB/msbB mutation, is attenuated in mice and induces significant protection from challenge with wild type S. Typhimurium (Liu et al., 2008).
  • Related Vaccine(s): Salmonella Typhimurium IppA/IppB/msbB
38. lppB
  • Gene Name : lppB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
  • NCBI Protein GI : 267993263
  • Other Database IDs : CDD:187093
  • Taxonomy ID : 588858
  • Gene Strand (Orientation) : ?
  • Protein Name : putative methyl-accepting chemotaxis protein
  • Protein Length : 79
  • Protein Note : Murein lipoprotein [Cell envelope biogenesis, outer membrane]; cl11539
  • Protein Sequence : Show Sequence 
    >gi|267993263|gb|ACY88148.1| putative methyl-accepting chemotaxis protein [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]
MNRTNQLILGAVVLGSTLLAGCSSNAKIDQLSSDVQTLSAKVEQLSNDVNAMRSDVQAAKDDAARANQRL
DNKVFRICK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An lppB mutant, in combination with an msbB mutant or an msbB/lppA mutant, is attenuated in mice and induces significant protection from challenge with wild type S. Typhimurium (Liu et al., 2008).
  • Related Vaccine(s): Salmonella Typhimurium IppA/IppB/msbB , Salmonella Typhimurium IppB/msbB mutant vaccine
39. msbB
  • Gene Name : msbB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium 14028
  • NCBI Protein GI : 4104731
  • Other Database IDs : ATCC:14028
    CDD:153246
  • Taxonomy ID : 90371
  • Gene Strand (Orientation) : ?
  • Protein Name : lipid A acyltransferase
  • Protein Length : 323
  • Protein Note : Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: LABLAT-like; cd07984
  • Protein Sequence : Show Sequence 
    >gi|4104731|gb|AAD03801.1| lipid A acyltransferase [Salmonella enterica subsp. enterica serovar Typhimurium]
METKKNNSEYIPEFEKSFRYPQYWGAWLGAAAMAGIALTPASFRDPLLATLGRFAGRLGKSSRRRALINL
SLCFPQRSEAEREAIVDEMFATAPQAMAMMAELAMRGPKKIQQRVDWEGLEIIEEMRRNDEKVIFLVPHG
WGVDIPAMLMASQGQKMAAMFHNQGNPVFDYIWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQ
DHGPEHSEFVDFFATYKATLPAIGRLMKVCRARVIPLFPVYNGKTHRLTIQIRPPMDDLLTADDHTIARR
MNEEVEIFVGPHPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An msbB mutant, in combination with an lppB or lppAB mutation, is attenuated in mice and induces significant protection from challenge with S. Typhimurium (Liu et al., 2008).
  • Related Vaccine(s): Salmonella Typhimurium IppA/IppB/msbB , Salmonella Typhimurium IppB/msbB mutant vaccine
40. NmpC
  • Gene Name : NmpC
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • NCBI Protein GI : 517159
  • Other Database IDs : CDD:174088
  • Taxonomy ID : 90371
  • Gene Strand (Orientation) : ?
  • Protein Name : NmpC protein
  • Protein Length : 282
  • Protein Note : synonym: Salmonella typhimurium
  • Protein Sequence : Show Sequence 
    >gi|517159|dbj|BAA05056.1| NmpC protein [Salmonella enterica subsp. enterica serovar Typhimurium]
EFKGNRTESQGADKDKTRLAFAGLKFADYGSFDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRT
TGVATYRKTDFFGLVEGLNFAAQYQGKNDRDGAYESNGDGFGLSATYEYEGFGVGAAYAKSDRTNNQVKA
ASNLNAAGKNAEVWAAGLKYDANNIYLATTYSETLNMTFGEDAAGDAFIANKTQNFEAVAQYQFDFGLRP
SIAYLKSKGKNLGTYGDQDLVEYIDVGATYYFNKNMSTFVDYKINLLDDSDFTKAAKVSTDNIVAVGLNY
QF
  • Molecule Role : Virmugen
  • Molecule Role Annotation : In a pig infection experiment including two oral immunizations with SalmoporcDeltaompD (OmpD, nmpC) and challenge with a multiresistant S. enterica serovar Typhimurium DT104 clinical isolate, we confirmed the protective efficacy of SalmoporcDeltaompD in pigs, showing a significant reduction of both clinical symptoms and colonization of lymph nodes and intestinal tract (Selke et al., 2007).
  • Related Vaccine(s): Salmonella Typhimurium ompD/nmpC mutant vaccine
41. nuoG
  • Gene Name : nuoG
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6924283
  • NCBI Protein GI : 205353438
  • Locus Tag : SG2352
  • Genbank Accession : AM933173
  • Protein Accession : YP_002227239
  • Taxonomy ID : 550538
  • Gene Starting Position : 2416815
  • Gene Ending Position : 2419541
  • Gene Strand (Orientation) : -
  • Protein Name : NADH dehydrogenase subunit G
  • Protein pI : 6.17
  • Protein Weight : 93802.85
  • Protein Length : 908
  • Protein Note : Catalyzes the transfer of electrons from NADH to quinone
  • DNA Sequence : Show Sequence 
    >gi|205351346:2416815-2419541 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
CTCATTGTTGCGCCTCCCGCAGATCCTCAAGACGCGCGCCAGCCAGAACCGGCGCGATGCCAGGCATACC
CATCGGCAGCCCTACCTGCCCTGCCGCTAACCCTTCAGAGATTTCAACCGGCAGCGTCACCGTATTGCCA
TCGTAGCTAAAGGAGACGCGCGTCCCGGCATTGACGCCCAACTTCGCGGCATCCGCCGGGTTAAGTTTGA
TATACGGCTGCGGCATACGGCTCTGGAAGACCGGAGAACGCTGAGACAATCCGTCGCTGCCAAACAGGTG
GTAATACGGCGCAATACGCCACTGACCGTCCTGCGCCTGGAAGCTTGCCGGCACGGTAGTGAAATAATCC
AGACCGCCCTCAGTCGCTTCAATCAAACGCACGCCCGGATCGCCGTGACGCAGTTTGCCGCCCACTTCAT
CCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCGAACGGAATTTCAGATCGCGG
GGCAGTCGGTTGGTTATTCCCTTCCATCGAGAAGGCGAACATGGTGTCTTTATCCTGCGGCTGACGTGGT
TCATGCACGCTGATATTGGCGCGCATCGCCGTACGACCGCTGTAACGATGCGGTTCGCGCGCCAGCTTCT
GCCCACGAATGCGGAAGGTCGCATCCGGCGCGGCGTCTTTAATGCCGGCAAATTGCGGCATGGCGGCAAT
GACCGCGTCGATCACATGGTCAAGCTGTGTCCAGTCCACTTCGCGGTTTTCGACGGTGCTGTGCAGCGAA
TGCAGCCAGCGCCAGCTTTCCAGCATAATCGTCTTGTTATCGTAGTAGGCCGGATCATAAACCTGGAAGA
AGCGCTGCGCGCGGCCTTCGTTGTTGATGACCGTACCATCGCTTTCGGCGAAGCTTGCCGCGGAAAGCAC
CAGATGCGCGTTTTCCATAATCGCCGTGCGCTGATGGTCAACCACCATCACCAGCGGCGCTTTGGCGAGC
GCGGCGTTCACGCGCGTAGCGCTTGCATGACGGTGGAGATCGTTTTCCAGCACCACCACCGCGTCCGCGC
TGCCGGTTTCCAGCTCGCCCAACGCATCGTCAAGCGAACCGCCTCCCATCATACCCAGCCCCATGCTGTT
CACGGAGCGGGCAATCATAGTAATACCGACGTCGGCGCCACGGCCTTTCAGCGCTTTCGCCACGTTGGCG
GCCGCCTGAATCACCTCACTGCTACCGGCGTTAGTGCCGGAGATAATCAGCGGTTTTTTCGCGCCGGCCA
ACGCCTGCACAATGACATCAATTTTGTTTTGCAAGTCGCTGTCGATACCGTCAACCGCAGGCGCTGTATT
GTCCAGCGCATGGGCAATCGCAAAGCCCAGGCGCGCCTGATCTTCCACCGGCGCGCGATAGGTCCAGGCG
GCGATATCATCCAGACGGGTGTCGTCCACGTTGGTCACGAACAGCGGATGTTTGGCACGCTGGCCGATGT
TAAGGATGGCCGCAATCTGCCAGTCGGCCACTTTCTGCGCCGCCGCCATTTCACGCGCTTTACCTTTCAC
CGCCTGACGTACCGCCAGCGCAACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAAGACCAGTACCGCG
TCATAAGATTCGATTTCCCGCAGCGCCGGGGTGTAAATGCCGCCTTCACGCAGCACTTTCAGCGCCAGTT
GCAGACGTTCCTGCTCGCCGCGCGCGATACCGGTGTAGAAGTTTTCCGCACCAACCAGTTCACGCAGCGC
AAAGTTGCTCTCAATGCTGGCGCGTGGCGAACCGATACCGATCACTTTCTTCGACTGGCGCAGAATATCC
GCCGCGCCCTGCATCGCCTGCTCGGCGTTGAGGGTGATGAAGTCGTCGCCACGGCGCTGAACCGGCTGAC
GCGGACGGTCTTTCAGGTTCACATAGCCATAGCCGAAACGACCGCGGTCGCAGAGGAAGTAGTGGTTAAC
GGTACCGTTGTAACGGTTTTCGATACGGCGTAATTCGCCATAGCGCTCGCCCGGGCTGATGTTACAGCCG
ATGGAACACTGCTGGCAGATGCTCGGCGCAAACTGCATGTCCCATTTACGGTTGTAACGCTCGGAGTGCG
TTTTATCGGTGAAGACGCCGGTCGGGCAAATCTCGACCAGGTTACCGGAGAATTCGCTTTCCAGCGTACC
GTCTTCCGGACGCCCGAAGTAGACGTTGTCATGCGCGCCATAGACGCCCAGATCGGTGCCGTCGGCATAA
TCTTTGTAGTAACGCACACAACGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAACGGCCCCA
GATCCTGGTTGCGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAACTGTGACCCGTCATCACGGTCAT
ATCCTGAAGATGGCAGTTACCGCCCTCTTCACAGACCGGACAGTCGTGCGGGTGGTTAGTCATCAGCCAC
TCCACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATAGAGATAAAGGTACCGTCGGTGGCTG
GCGTCATACAGGACATCACCAGGCGACCCCGCGTATCTTCCGCGTTTTGGTATTGCTTCACCGCACACTG
GCGGCAAGCACCGACGCTTCCCAGCGCCGGGTGCCAGCAAAAATACGGAATATCAAGGCCGAGGGACAGA
CATGCCTGTAGCAGGTTGTCCGCGCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATCGTAGCCA
  • Protein Sequence : Show Sequence 
    >gi|205353438|ref|YP_002227239.1| NADH dehydrogenase subunit G [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
MATIHVDGKEYEVNGADNLLQACLSLGLDIPYFCWHPALGSVGACRQCAVKQYQNAEDTRGRLVMSCMTP
ATDGTFISIDDEEAKQFRESVVEWLMTNHPHDCPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDL
GPFISHEMNRCIACYRCVRYYKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKT
HSERYNRKWDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLKDRPR
QPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASIESNFALRELVGAENFYTGIARGEQERLQ
LALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALAVRQAVKGKAREMAAAQKVADWQIAAILN
IGQRAKHPLFVTNVDDTRLDDIAAWTYRAPVEDQARLGFAIAHALDNTAPAVDGIDSDLQNKIDVIVQAL
AGAKKPLIISGTNAGSSEVIQAAANVAKALKGRGADVGITMIARSVNSMGLGMMGGGSLDDALGELETGS
ADAVVVLENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNEGRAQRF
FQVYDPAYYDNKTIMLESWRWLHSLHSTVENREVDWTQLDHVIDAVIAAMPQFAGIKDAAPDATFRIRGQ
KLAREPHRYSGRTAMRANISVHEPRQPQDKDTMFAFSMEGNNQPTAPRSEIPFAWAPGWNSPQAWNKFQD
EVGGKLRHGDPGVRLIEATEGGLDYFTTVPASFQAQDGQWRIAPYYHLFGSDGLSQRSPVFQSRMPQPYI
KLNPADAAKLGVNAGTRVSFSYDGNTVTLPVEISEGLAAGQVGLPMGMPGIAPVLAGARLEDLREAQQ
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A nuoG mutant is attenuated in chickens and induced significant protection from challenge with wild type S. Gallinarum (Zhang-Barber et al., 1998).
  • Related Vaccine(s): Salmonella Gallinarum nuoG mutant vaccine
42. ompR
  • Gene Name : ompR
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
  • NCBI Protein GI : 301160045
  • Other Database IDs : CDD:181883
    CDD:29071
    CDD:29475
    UniProtKB/TrEMBL: E1WIS9
  • Taxonomy ID : 216597
  • Gene Strand (Orientation) : ?
  • Protein Name : two-component response regulator OmpR
  • Protein Length : 239
  • Protein Note : osmolarity response regulator; Provisional; PRK09468
  • Protein Sequence : Show Sequence 
    >gi|301160045|emb|CBW19564.1| two-component response regulator OmpR [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLLTRESFHLMVLDLMLPGEDGLSICRRL
RSQSNPMPIIMVTAKGEEVDRIVGLEIGADDYIPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAF
GKFKLNLGTREMFREDEPMPLTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRR
MVEEDPAHPRYIQTVWGLGYVFVPDGSKA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An ompR mutant is attenuated in mice after oral challenge. Twenty eight days after immunization, mice were challenged with wild type S. typhimurium and were significantly protected (Dorman et al., 1989).
  • Related Vaccine(s): Salmonella Typhimurium ompR mutant vaccine
43. PhoP
  • Gene Name : PhoP
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. Ty2
  • NCBI Gene ID : 1071307
  • NCBI Protein GI : 29142123
  • Locus Tag : t1689
  • Genbank Accession : AE014613
  • Protein Accession : NP_805465
  • Taxonomy ID : 209261
  • Gene Starting Position : 1752334
  • Gene Ending Position : 1753008
  • Gene Strand (Orientation) : +
  • Protein Name : DNA-binding transcriptional regulator PhoP
  • Protein pI : 5.16
  • Protein Weight : 23440.94
  • Protein Length : 224
  • Protein Note : response regulator in two-component regulatory system with PhoQ; involved in magnesium starvation and stress
  • DNA Sequence : Show Sequence 
    >gi|29140543:1752334-1753008 Salmonella enterica subsp. enterica serovar Typhi str. Ty2, complete genome
GATGATGCGCGTACTGGTTGTAGAGGATAATGCATTATTACGCCACCACCTGAAGGTTCAGCTCCAGGAT
TCAGGTCACCAGGTCGATGCCGCAGAAGATGCCAGGGAAGCTGATTACTACCTTAATGAACACCTTCCGG
ATATCGCTATTGTCGATTTAGGTCTGCCGGATGAAGACGGCCTTTCCTTAATACGCCGCTGGCGCAGCAG
TGATGTTTCACTGCCGGTTCTGGTGTTAACCGCGCGCGAAGGCTGGCAGGATAAAGTCGAGGTTCTCAGC
TCCGGGGCCGATGACTACGTGACGAAGCCATTCCACATCGAAGAGGTAATGGCGCGTATGCAGGCGTTAA
TGCGCCGTAATAGCGGTCTGGCCTCCCAGGTGATCAACATCTCGCCTTTCCAGGTGGATCTCTCACGCCG
GGAATTATCCGTCAATGAAGAGGTCATCAAACTCACGGCGTTCGAATACACCATTATGGAAACGCTTATC
CGTAACAACGGTAAAGTGGTCAGCAAAGATTCTCTGATGCTTCAGCTGTATCCGGATGCGGAACTGCGGG
AAAGTCATACCATTGATGTTCTCATGGGGCGTCTGCGGAAAAAAATACAGGCCCAGTATCCGCACGATGT
CATTACCACCGTACGCGGACAAGGATATCTTTTTGAATTGCGCTA
  • Protein Sequence : Show Sequence 
    >gi|29142123|ref|NP_805465.1| DNA-binding transcriptional regulator PhoP [Salmonella enterica subsp. enterica serovar Typhi str. Ty2]
MMRVLVVEDNALLRHHLKVQLQDSGHQVDAAEDAREADYYLNEHLPDIAIVDLGLPDEDGLSLIRRWRSS
DVSLPVLVLTAREGWQDKVEVLSSGADDYVTKPFHIEEVMARMQALMRRNSGLASQVINISPFQVDLSRR
ELSVNEEVIKLTAFEYTIMETLIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPHDV
ITTVRGQGYLFELR
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Three S. typhi deletion mutants (phoP, rpoS, and rpoS-phoP double mutant) were constructed and characterized with respect to invasiveness, virulence, and protective immune response compared with wild-type Ty2. All S. typhi mutants showed an increase in the specific serum IgG levels and T-cell-mediated immunity, and showed equal protection abilities against a wild-type Ty2 challenge after two rounds of immunization in BALB/c mice (Lee et al., 2007).
  • Related Vaccine(s): Salmonella Typhi phoP mutant vaccine
44. poxA
  • Gene Name : poxA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1255870
  • NCBI Protein GI : 16767593
  • Locus Tag : STM4344
  • Genbank Accession : AE006468
  • Protein Accession : NP_463208
  • 3D structure: PDB ID : 3G1Z
  • Taxonomy ID : 700720
  • Gene Starting Position : 4586094
  • Gene Ending Position : 4587071
  • Gene Strand (Orientation) : +
  • Protein Name : lysyl-tRNA synthetase
  • Protein pI : 4.92
  • Protein Weight : 35366.49
  • Protein Length : 325
  • Protein Note : lysine--tRNA ligase beta chain; poxA; class II aminoacyl tRNA synthetase; catalyzes a two-step reaction; first charging a lysine molecule by linking the carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA
  • DNA Sequence : Show Sequence 
    >gi|16763390:4586094-4587071 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
CATGAGCGAAACGGCAACCTGGCAGCCGAGCGCGTCCATCCCCAATTTATTAAAACGTGCGGCGATTATG
GCGGAAATCCGTCGTTTCTTTGCCGATCGTGGAGTGCTTGAGGTTGAGACGCCCTGCATGAGTCAGGCGA
CGGTCACAGACATTCATCTGTTCCCGTTCGAAACGCGTTTCGTCGGACCTGGCCATTCCCAGGGGATCAA
CCTCTATTTAATGACCAGTCCGGAATACCATATGAAACGCCTGCTGGCGGCAGGGTGCGGCCCGGTTTTC
CAGCTATGCCGCAGTTTCCGTAATGAAGAGATGGGACGACATCATAATCCGGAATTCACTATGCTGGAGT
GGTATCGCCCGCATTACGATATGTACCGCCTGATGAATGAAGTGGATGATTTGCTTCAGCAAGTGCTGGA
TTGTCAACCTGCGGAAAGTCTCTCCTATCAACAGGCGTTTCAGCGCCATCTGGAGATTGACCCGTTATCA
GCAGATAAAACGCAACTGCGTGAGGCGGCGGCAAAGCTTGATTTAAGCAATATCGCCGATACGGAAGAAG
ACCGTGATACGTTGCTGCAACTGTTGTTCACGATGGGGGTTGAGCCGCATATAGGTAAAGAAAAGCCGAC
CTTTATTTATCACTTTCCGGCAAGTCAGGCATCGCTGGCACAAATCAGTACCGAGGATCATCGCGTCGCC
GAGCGCTTTGAGGTGTACTACAAAGGTATTGAGCTGGCGAATGGTTTCCACGAACTGACGGACGCACGTG
AGCAACAACAGCGCTTTGAACAGGACAATCGTAAGCGCGCCGCTCGCGGTCTGCCGCAGCAGCCGATTGA
CCAAAATCTACTGGATGCGCTGGCCGCCGGTCTACCGGATTGTTCCGGCGTGGCGCTGGGTGTTGATCGT
CTGGTGATGCTGGCGCTGGGAGCAGAAAGCCTGGCGGACGTGATTGCTTTTACGGTCGATCGGGCGTA
  • Protein Sequence : Show Sequence 
    >gi|16767593|ref|NP_463208.1| lysyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MSETATWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLFPFETRFVGPGHSQGIN
LYLMTSPEYHMKRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLD
CQPAESLSYQQAFQRHLEIDPLSADKTQLREAAAKLDLSNIADTEEDRDTLLQLLFTMGVEPHIGKEKPT
FIYHFPASQASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQQPID
QNLLDALAAGLPDCSGVALGVDRLVMLALGAESLADVIAFTVDRA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A poxA mutant is attenuated in mice and induces significant protection from challenge with wild type S. typhimurium (Kaniga et al., 1998).
  • Related Vaccine(s): Salmonella Typhimurium poxA mutant vaccine
45. relA
  • Gene Name : relA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6922678
  • NCBI Protein GI : 205353902
  • Locus Tag : SG2866
  • Genbank Accession : AM933173
  • Protein Accession : YP_002227703
  • Taxonomy ID : 550538
  • Gene Starting Position : 2982005
  • Gene Ending Position : 2984239
  • Gene Strand (Orientation) : -
  • Protein Name : GDP/GTP pyrophosphokinase
  • Protein pI : 6.78
  • Protein Weight : 78259.88
  • Protein Length : 744
  • Protein Note : (p)ppGpp synthetase; catalyzes the formation of pppGpp and ppGpp from ATP and GTP or GDP
  • DNA Sequence : Show Sequence 
    >gi|205351346:2982005-2984239 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
TTTACCCCCCGTGCAGTCGCCGTGCATCAATCACATCCGGCACCTGGTTCAGCTTACCGAGCACGCGGCC
CAGCACCTGCAGGTTGTAGATCTCGATGGTCATATCAATGGTGGCGATCTGCTGTTTAATGTCGCTGCGG
CTGGCGACGCCCAGCACGTTGACTTTTTCGTTAGCCAGAATAGTGGTGATATCGCGCAGCAAGCCGCTGC
GATCGTTGGCCTGGACGCGCACCACCAGCGAATATCCCGCCGAGTAGCTTTCGCCCCATACCGCCTCTAC
GATCCGCTCCGGCGCATGGGAGCGCAGTTCCGCCAGCTGTTCGCAGTCGGCCCGGTGCACGGAAATTCCC
CGCCCCTGAGTAATGAAGCCGACAATTTCATCGCCAGGAATCGGCTGGCAGCAGCGGGCAATGTGGTGCA
TCAGGTTACCAACCCCTTCCACCACCACGCGCCCGTCGTCTTTACGACGATTTTGCGGCGCGTATGTTTT
TTGCTGAAGCTGTTTCAGCGCCGCTGCATCCTGCTCCTCCGCACTCGGCTTATTGAACTGCGATTGCAGG
AAATTCACCATCTGATTAAGACGGATATCGCCGCCGCCTATCGCCGCCAGCAACTCTTCCAGCTCATTAA
AGTTGTAGCGCGGCAGCAGATGTTTTTCGGCCTCTTTCAGGCTAATCCCCAAATGCGCCAGCTCATCGTC
GAGGATCTGCCGTCCAGCCTGGATATTTTTGTCACGATCCTGCTTGCGGAACCAGGCGTGAATTTTCGAG
CGTCCGCGGCTGGTCGTCACATAGCCCAGGTTTGGATTCAGCCAGTCGCGGCTGGGATTCGGCTGCTTCT
GAGTGATAATTTCAATTTGATCACCCATCTGCAACTGATAGGTGAATGGCACAATACGGCCGCCGATTTT
AGCGCCAATGCAGCGGTGCCCAACATCGCTGTGGATGTGGTAAGCAAAATCGAGCGGCGTAGATCCGGCA
GGCAAGTCAACCACGTCGCCTTTTGGCGTAAAAACGTAGACCCGATCGTCAAACACCTGGCTGCGCACTT
CATCCAGCATTTCGCCGGAATCGGCCATCTCTTCCTGCCAGGCGATCAGCTTACGCAGCCACGCAATTCT
GTCTTCATGACCGGAGCGCACGCCGCCGGACGCGGCGCCTTCTTTATACTTCCAGTGTGCCGCCACGCCC
AGTTCGGCGTCTTCATGCATCTGTTTGGTACGGATCTGGATCTCAACGGTTTTACCGCCCGGTCCCAGGA
CCACGGTGTGGATAGACTGGTAACCGTTCGGTTTCGGGTTAGCGACATAATCATCGAATTCATCCGGCAG
GTGACGATAGTGCGTATGCACTATCCCCAACGCGGCGTAGCAGTCCTGCAGACGTTCAGCGACAATACGC
ACGGCGCGCACGTCAAAGAGTTCATCAAACGCCAGATGCTTTTTCTGCATTTTGCGCCAGATGCTATAAA
TATGTTTTGGTCGTCCGTAGACCTCCGCCTGCACGCCTTCGTTTTTCATTTCGGCGCGCAGATGTCCAAC
AAACTCTTCGATGTAATGTTCGCGATCGAGACGGCGCTCATGCAGCAGTTTGGCGATGCGTTTGTATTCC
GCCGGATGCAGGTAGCGGAAACAGTAGTCTTCCAGTTCCCACTTCAGTTGCCCGATGCCCAGACGATTAG
CGAGCGGCGCATAGATGTTGGTACATTCTTTCGCCGCCAGCACGCGCTCATCTTCCGGCGCCTCTTTCAC
TTCGCGCAAATGAGCGATTCGCTCGGCCAGTTTGATCACCACGCAGCGGAAATCATCCACCATCGCCAAT
AACATTCGACGGACGTTATCAACCTGCTCCGAAGAAACAGAGTCGTTATGAGTGGCGTTTAGCTGACGGA
TCGCCGCCATATCGCGCACGCCATGAATCAGGGTAACGATAGATTTGCCGACGCTTTCGCGCAGTACATC
TTCGCTGACTACGTTGGCGTCGGCCAGAGGGAACAGTAGCGCCGCCCGCAGCGTGTCGATATCCATACTC
AGCGTGGAAAGAATTTCTACCATCTCCACGCCACGCCACAGCAACAGATCCGCATCCGGATGTCCTTGTG
TCTGTTGCAGGCAATACGCCCAGGTTTCGGCTAAGCGCTCACACGACTGCTGGCTGGAAATTCCCAGGCT
TGCGATCCACTTCTTCGGATCAAATTCACCAGCTTTATTAATATGTGCACTTCTTACCGCGACCA
  • Protein Sequence : Show Sequence 
    >gi|205353902|ref|YP_002227703.1| GDP/GTP pyrophosphokinase [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
MVAVRSAHINKAGEFDPKKWIASLGISSQQSCERLAETWAYCLQQTQGHPDADLLLWRGVEMVEILSTLS
MDIDTLRAALLFPLADANVVSEDVLRESVGKSIVTLIHGVRDMAAIRQLNATHNDSVSSEQVDNVRRMLL
AMVDDFRCVVIKLAERIAHLREVKEAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPAE
YKRIAKLLHERRLDREHYIEEFVGHLRAEMKNEGVQAEVYGRPKHIYSIWRKMQKKHLAFDELFDVRAVR
IVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTVEIQIRTKQMHEDAELG
VAAHWKYKEGAASGGVRSGHEDRIAWLRKLIAWQEEMADSGEMLDEVRSQVFDDRVYVFTPKGDVVDLPA
GSTPLDFAYHIHSDVGHRCIGAKIGGRIVPFTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRS
KIHAWFRKQDRDKNIQAGRQILDDELAHLGISLKEAEKHLLPRYNFNELEELLAAIGGGDIRLNQMVNFL
QSQFNKPSAEEQDAAALKQLQQKTYAPQNRRKDDGRVVVEGVGNLMHHIARCCQPIPGDEIVGFITQGRG
ISVHRADCEQLAELRSHAPERIVEAVWGESYSAGYSLVVRVQANDRSGLLRDITTILANEKVNVLGVASR
SDIKQQIATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARRLHGG
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A ppGpp-defective Salmonella Gallinarum, which is attained by mutating the relA and spoT genes, is attenuated in chickens and induces protection from oral challenge with wild type S. Gallinarum (Park et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum relA/spoT mutant vaccine
46. rfc
  • Gene Name : rfc
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
  • NCBI Protein GI : 267993210
  • Other Database IDs : CDD:33116
    CDD:186638
  • Taxonomy ID : 588858
  • Gene Strand (Orientation) : ?
  • Protein Name : O-antigen polymerase
  • Protein Length : 407
  • Protein Note : O-antigen polymerase (SW:RFC_SALTY)
  • Protein Sequence : Show Sequence 
    >gi|267993210|gb|ACY88095.1| O-antigen polymerase [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]
MLIISYIALCLLFIVYLYTLSVRIEGKIINVMVPYLIITVPTLYVFEGIFVYLSEVQNYTVEYLFFYTCY
ITYIASFVISYLYTQRKPIYNKSNTKNKPRYVFTSLLFTFLAFIIYLPVLMEFREYILSPRRIYELTRTG
YGIYFYPSLMFSLVASICAFFTYKKSKLFCISIVLFNCILIFLHGNKGPIFSIFIAFILYLSYIENKKIK
FMFLVKSFAVIAVIVTAFFAYTFTDGNPIENMANYSDYTRNAVLVASSNFDFMYGKLLMESEVYSRIPRA
IWPDKPEDFGALYLAKVFFPDAFYRNQGAPAFGYGELYADFGLFTPVWLVISGVFKGVLAKYFSNKTQET
KSAHYFIMFLFCIGISVIPVSMGWLFPEHLMIAFMVYIASSFVFSEHIRFVLLRNNK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : An rfc mutant, in combination with an asd mutation, is attenuated in mice and induces significant protection from challenge with wild type S. typhimurium (Piao et al., 2010).
  • Related Vaccine(s): Salmonella Typhimurium asd/rfc mutant vaccine
47. rpoB
  • Gene Name : rpoB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1255679
  • NCBI Protein GI : 16767407
  • Locus Tag : STM4153
  • Genbank Accession : AF170176
  • Protein Accession : NP_463022
  • Taxonomy ID : 700720
  • Gene Starting Position : 4365907
  • Gene Ending Position : 4369935
  • Gene Strand (Orientation) : +
  • Protein Name : DNA-directed RNA polymerase subunit beta
  • Protein pI : 4.89
  • Protein Weight : 139687.68
  • Protein Length : 1342
  • Protein Note : DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme
  • DNA Sequence : Show Sequence 
    >gi|16763390:4365907-4369935 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
TATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGAT
GTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAGTTTATCGAGCAAGATCCTGAAGGGCAGT
ACGGCCTGGAAGCAGCCTTCCGTTCCGTGTTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAGTA
CGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTTCAGGAATGTCAGATCCGTGGCGTGACCTATTCC
GCACCGCTGCGCGTAAAACTGCGTCTGGTGATCTACGAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACA
TTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACCGACAACGGTACCTTTGTTATCAACGG
TACTGAGCGTGTTATCGTTTCTCAGCTGCACCGTAGCCCTGGCGTCTTCTTTGACTCCGACAAAGGTAAA
ACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGCATCATTCCTTACCGTGGTTCCTGGCTGGACTTCG
AATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTACCTGCGACCATCATCCT
GCGTGCGCTGAACTACACCACTGAGCAGATCCTTGACCTGTTCTTTGAGAAAGTCGTCTTCGAAATTCGC
GACAACAAACTGCAGATGGAGCTGATTCCAGAACGTCTGCGTGGCGAAACCGCGTCGTTCGATATCGAAG
CTAACGGCAAAGTGTACGTTGAAAAAGGCCGTCGCATTACCGCGCGTCACATCCGTCAGCTGGAAAAAGA
CGATATCAAGCATATCGAAGTTCCGGTTGAGTACATCGCAGGTAAAGTCGTCTCTAAAGACTACGTTGAC
GAATCTACTGGCGAGCTGATCTGCGCGGCGAACATGGAGCTGAGCCTGGATCTGCTGGCTAAGCTGAGCC
AGTCCGGCCACAAGCGTATCGAAACGCTGTTTACCAACGATCTGGACCACGGCCCGTACATTTCTGAAAC
GGTACGCGTCGACCCAACTAACGATCGTCTGAGCGCGCTGGTAGAAATCTACCGCATGATGCGCCCTGGT
GAGCCGCCGACACGCGAAGCGGCTGAAAGCCTGTTTGAGAACCTGTTCTTCTCCGAAGACCGCTATGACC
TGTCTGCGGTGGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGACGAAATCGAAGGTTCCGGTATCCT
GAGCAAAGACGACATCATTGATGTCATGAAGAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTGGAT
GATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTCGGCGAAATGGCGGAAAACCAGTTCCGCGTTG
GCCTGGTGCGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCTCA
GGATATGATCAACGCCAAGCCGATTTCCGCCGCAGTGAAAGAGTTCTTTGGTTCCAGCCAGCTGTCTCAG
TTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCG
GTCTGACCCGCGAACGCGCAGGCTTCGAAGTTCGAGACGTACACCCGACGCACTACGGTCGCGTATGTCC
TATCGAAACGCCGGAAGGTCCGAACATCGGTCTGATCAACTCCCTGTCCGTGTACGCACAGACTAACGAA
TATGGCTTCCTTGAGACGCCGTACCGTCGTGTGGTTGATGGCGTGGTTACTGACGAAATTCATTACCTGT
CTGCTATCGAAGAAGGCAACTACGTTATCGCTCAGGCGAACTCCAACCTGGATGACGAAGGCCACTTTGT
AGAAGATTTGGTGACCTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATG
GACGTTTCCACCCAGCAGGTGGTCTCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCA
ACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCGGTTCCGACTCTGCGCGCTGACAAGCCGCTGGT
GGGTACCGGTATGGAACGTGCTGTCGCCGTCGACTCCGGTGTTACTGCAGTAGCTAAACGTGGCGGTACT
GTTCAGTACGTGGATGCGTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTACCCGGGCGAAGCGG
GTATCGACATCTATAACCTGACCAAATACACCCGCTCTAACCAGAACACCTGTATCAACCAGATGCCATG
TGTGTCTCTGGGCGAGCCGGTTGAACGCGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAA
CTGGCGCTCGGTCAGAACATGCGCGTGGCGTTCATGCCGTGGAACGGCTACAACTTCGAAGACTCCATTC
TCGTTTCCGAGCGTGTTGTCCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGCGTGTC
CCGTGACACCAAGCTGGGGCCGGAAGAGATCACCGCTGATATCCCGAACGTGGGTGAAGCTGCGCTCTCC
AAACTGGATGAATCCGGTATCGTTTACATCGGCGCGGAAGTGACAGGCGGTGACATTCTGGTCGGTAAGG
TGACGCCGAAAGGTGAAACCCAGCTGACGCCGGAAGAGAAACTGCTGCGTGCGATCTTCGGTGAGAAAGC
GTCTGACGTTAAAGACTCTTCTCTGCGCGTACCTAACGGTGTCTCCGGTACGGTTATCGACGTTCAGGTC
TTTACTCGCGATGGCGTAGAAAAAGACAAACGTGCTCTGGAAATCGAAGAGATGCAGCTCAAGCAGGCGA
AAAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGCCTGTTTAGCCGTATCCGTGCGGTGCTGGT
TTCCAGCGGCGTTGAAGCTGAGAAGCTCGACAAACTGCCGCGCGACCGCTGGCTGGAACTCGGTCTGACC
GACGAAGAGAAACAAAATCAGCTGGAACAACTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGA
AAAAACTTGAAGCGAAGCGCCGTAAAATCACCCAGGGCGACGATCTGGCGCCGGGCGTGCTGAAGATCGT
GAAGGTCTATCTGGCCGTTAAACGCCGTATCCAGCCGGGTGATAAGATGGCAGGTCGTCACGGTAACAAG
GGTGTTATCTCTAAGATCAACCCGATCGAAGACATGCCTTACGATGAAAACGGCACGCCGGTAGACATCG
TACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATTCTGGAAACCCACCTGGGTATGGC
TGCGAAAGGTATTGGCGACAAGATTAACGCCATGCTGAAACAGCAGCAGGAAGTCGCGAAACTGCGCGAA
TTCATCCAGCGTGCCTACGATCTGGGCGCCGACGTTCGTCAGAAAGTCGACCTGAGCACCTTCAGCGATG
ATGAAGTGCTGCGTCTGGCAGAAAACCTGCGCAAAGGTATGCCGATTGCCACACCAGTGTTTGACGGTGC
GAAAGAAGCGGAAATCAAAGAGCTGCTGAAACTGGGCGACCTGCCGACCTCCGGTCAGATCACCCTGTTC
GATGGCCGTACGGGTGAACAGTTCGAACGTCCGGTAACCGTCGGTTACATGTACATGCTGAAACTGAACC
ACCTGGTTGATGACAAGATGCATGCGCGTTCTACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGG
TGGTAAGGCGCAGTTCGGTGGTCAGCGCTTCGGGGAGATGGAAGTGTGGGCGCTGGAAGCTTACGGCGCC
GCCTACACCCTGCAGGAAATGCTCACCGTTAAGTCTGATGACGTGAATGGTCGTACCAAGATGTATAAGA
ACATCGTGGACGGCAACCATCAGATGGAACCGGGTATGCCGGAATCCTTCAACGTTCTGTTGAAAGAGAT
CCGCTCGCTGGGCATCAACATCGAACTGGAAGACGAGTA
  • Protein Sequence : Show Sequence 
    >gi|16767407|ref|NP_463022.1| DNA-directed RNA polymerase subunit beta [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSYSGNSELQY
VSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVING
TERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIIL
RALNYTTEQILDLFFEKVVFEIRDNKLQMELIPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKD
DIKHIEVPVEYIAGKVVSKDYVDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISET
VRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLRDEIEGSGIL
SKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQ
DMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCP
IETPEGPNIGLINSLSVYAQTNEYGFLETPYRRVVDGVVTDEIHYLSAIEEGNYVIAQANSNLDDEGHFV
EDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLV
GTGMERAVAVDSGVTAVAKRGGTVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPC
VSLGEPVERGDVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVS
RDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKA
SDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLFSRIRAVLV
SSGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIV
KVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMA
AKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDDEVLRLAENLRKGMPIATPVFDGA
KEAEIKELLKLGDLPTSGQITLFDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLG
GKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPESFNVLLKEI
RSLGINIELEDE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A gyrA-cpxA-rpoB Salmonella Typhimurium mutant was attenuated and protected against challenge in pigs (Roesler et al., 2004).
  • Related Vaccine(s): Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
48. rpoS
  • Gene Name : rpoS
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium SL1344
  • NCBI Protein GI : 301159483
  • Other Database IDs : CDD:180185
    CDD:189414
    CDD:146937
    CDD:146934
    CDD:100119
    UniProtKB/TrEMBL: E1WAG8
  • Taxonomy ID : 216597
  • Gene Strand (Orientation) : ?
  • Protein Name : RNA polymerase sigma subunit RpoS (sigma-38)
  • Protein Length : 330
  • Protein Note : RNA polymerase sigma factor RpoS; Validated
  • Protein Sequence : Show Sequence 
    >gi|301159483|emb|CBW19002.1| RNA polymerase sigma subunit RpoS (sigma-38) [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
MSQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPL
LTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGF
RFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVS
RMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGL
LGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE
  • Molecule Role : Virmugen
  • Molecule Role Annotation : Immunizations with Salmonella typhimurium rpoS and rpoS aroA mutants protected susceptible BALB/c mice against subsequent oral challenge with virulent S. typhimurium. When administered orally or intraperitoneally, rpoS derivatives of the mouse-virulent S. typhimurium strains, C52 and SL1344, were highly attenuated and were efficient single-dose live vaccines (Coynault et al., 1996).
  • Related Vaccine(s): Salmonella Typhimurium rpoS mutant vaccine
49. ruvB
  • Gene Name : ruvB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253415
  • NCBI Protein GI : 16765236
  • Locus Tag : STM1894
  • Genbank Accession : AE006468
  • Protein Accession : NP_460851
  • Taxonomy ID : 700720
  • Gene Starting Position : 1989053
  • Gene Ending Position : 1990063
  • Gene Strand (Orientation) : -
  • Protein Name : Holliday junction DNA helicase RuvB
  • Protein pI : 5.03
  • Protein Weight : 34934.59
  • Protein Length : 336
  • Protein Note : promotes strand exchange during homologous recombination; RuvAB complex promotes branch migration; RuvABC complex scans the DNA during branch migration and resolves Holliday junctions at consensus sequences; forms hexameric rings around opposite DNA arms; requires ATP for branch migration and orientation of RuvAB complex determines direction of migration
  • DNA Sequence : Show Sequence 
    >gi|16763390:1989053-1990063 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
GCTAAGGCATCTCTGGCGGCGTGATCCCGAAATGGTTCCAGGCGCGCACCGTTGCCATACGCCCACGCGG
CGTACGCTGCAAAAAGCCTTGCTGAATCAGATACGGCTCCAGCACATCCTCAATGGTTTCACGCTCTTCG
CCAATCGCCGCCGCCAGGTTATCCAGTCCCACCGGACCACCGAAGAATTTATCAATAACCGCCAGCAGCA
GCTTGCGATCCATATAGTCAAACCCTTCCGCATCGACGTTCAGCATATCCAGCGCCTGCGCGGCAATTTC
CGCTGAAATAGCCCCGTCATGCTTCACCTCAGCAAAATCACGCACACGTCTGAGCAGACGGTTGGCTATA
CGCGGCGTACCGCGCGCCCGACGCGCCACTTCCAGCGCGCCGTCATCGCTCATCTCAAGCCCCATGTGCC
TTGCGCTGCGCCCAACGATGTGCTGCAGATCGGGCACCTGGTAGAACTCCAGGCGCTGCACAATACCAAA
ACGGTCACGTAGCGGGGACGTTAACGACCCGGCGCGCGTCGTCGCGCCAATGAGCGTAAACGGCGGCAGA
TCGATCTTAATTGAACGTGCGGCAGGCCCTTCGCCAATCATAATATCCAACTGGTAATCTTCCATCGCCG
GATAGAGTACCTCTTCCACCACCGGTGAAAGCCGGTGAATCTCATCAATAAACAGCACATCATGCGGTTC
AAGATTGGTGAGCATAGCCGCCAGATCGCCTGCTTTTTCCAGCACCGGGCCGGACGTGGTTCGGAGATTG
ACGCCCATTTCATTGGCAACGATATTCGCCAGAGTCGTTTTACCTAACCCTGGCGGGCCGAAAATCAGCA
AGTGATCTAGTGCGTCGCCACGCCGTTTTGCCGCCTGAATAAAGATCTCCATCTGGGAGCGCACCTGCGG
TTGGCCGACATATTCCGCCAATAACTTCGGGCGAATGGCGCGATCGGCGACGTCTTCTGCGATCGTGGCG
CCTGCTGAAATCAGGCGATCTGCTTCTATCA
  • Protein Sequence : Show Sequence 
    >gi|16765236|ref|NP_460851.1| Holliday junction DNA helicase RuvB [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MIEADRLISAGATIAEDVADRAIRPKLLAEYVGQPQVRSQMEIFIQAAKRRGDALDHLLIFGPPGLGKTT
LANIVANEMGVNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIG
EGPAARSIKIDLPPFTLIGATTRAGSLTSPLRDRFGIVQRLEFYQVPDLQHIVGRSARHMGLEMSDDGAL
EVARRARGTPRIANRLLRRVRDFAEVKHDGAISAEIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGP
VGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPEMP
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A ruvB mutant is attenuated in mice and induced significant protection from challenge with wild type S. Typhimurium (Choi et al., 2010).
  • Related Vaccine(s): Salmonella Typhimurium ruvB mutant vaccine
50. sopB
  • Gene Name : sopB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • VO ID : VO_0010999
  • NCBI Protein GI : 54036439
  • Other Database IDs : CDD:283555
  • Taxonomy ID : 99287
  • Gene Strand (Orientation) : ?
  • Protein Name : Inositol phosphate phosphatase SopB
  • Protein pI : 9.82
  • Protein Weight : 60842.7
  • Protein Length : 682
  • Protein Note : Effector protein SopB
  • Protein Sequence : Show Sequence 
    >sp|O30916.2|SOPB_SALTY RecName: Full=Inositol phosphate phosphatase SopB; AltName: Full=Effector protein SopB
MQIQSFYHSASLKTQEAFKSLQKTLYNGMQILSGQGKAPAKAPDARPEIIVLREPGATWGNYLQHQKASN
HSLHNLYNLQRDLLTVAATVLGKQDPVLTSMANQMELAKVKADRPATKQEEAAAKALKKNLIELIAARTQ
QQDGLPAKEAHRFAAVAFRDAQVKQLNNQPWQTIKNTLTHNGHHYTNTQLPAAEMKIGAKDIFPSAYEGK
GVCSWDTKNIHHANNLWMSTVSVHEDGKDKTLFCGIRHGVLSPYHEKDPLLRHVGAENKAKEVLTAALFS
KPELLNKALAGEAVSLKLVSVGLLTASNIFGKEGTMVEDQMRAWQSLTQPGKMIHLKIRNKDGDLQTVKI
KPDVAAFNVGVNELALKLGFGLKASDSYNAEALHQLLGNDLRPEARPGGWVGEWLAQYPDNYEVVNTLAR
QIKDIWKNNQHHKDGGEPYKLAQRLAMLAHEIDAVPAWNCKSGKDRTGMMDSEIKREIISLHQTHMLSAP
GSLPDSGGQKIFQKVLLNSGNLEIQKQNTGGAGNKVMKNLSPEVLNLSYQKRVGDENIWQSVKGISSLIT
S
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : The entire SopB and SifB genes were cloned into DNA vaccine vectors and were administered along with live attenuated Salmonella. It was found that SopB vaccination reduced the bacterial burden of organs by about 5-fold on day 4 and day 8 after challenge with virulent Salmonella and proved to be a more efficient vaccination strategy than live attenuated bacteria alone (Nagarajan et al., 2009).
  • Related Vaccine(s): Salmonella DNA vaccine encoding SopB Protein
51. spoT
  • Gene Name : spoT
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
  • NCBI Gene ID : 6924903
  • NCBI Protein GI : 205354656
  • Locus Tag : SG3689
  • Genbank Accession : AM933173
  • Protein Accession : YP_002228457
  • Taxonomy ID : 550538
  • Gene Starting Position : 3881550
  • Gene Ending Position : 3883661
  • Gene Strand (Orientation) : -
  • Protein Name : bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase
  • Protein pI : 8.89
  • Protein Weight : 74653.38
  • Protein Length : 703
  • Protein Note : 3.1.7.2
  • DNA Sequence : Show Sequence 
    >gi|205351346:3881550-3883661 Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 chromosome, complete genome
GCTAGTTTCGGTTACGGGTGACTTTAATAACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCAG
ATGTACGCGATCGCGTGCGGTAAGGCGAATAAAGGTACTATAGACGCGACCATCTTTCTCTTCAGTATTC
AGGCTTTGAATATTGGAGGTGGTGGTATTAATCGCCGCCGTCAGGTTAGCCAGCGCGCCCTGATGGTTAA
ACATTTCCACCTTGATTTCGGTAATGAATTCCTGCTCCGTCTCTTTGTCCCATTCGACCGCCATAAATTT
CTCTGGCTCTTTCTGGTATCCACGGATATTACGGCAGGATTCGTGGTGGATCACCAGTCCTTTACCTGGG
CTGACGTGAGCGATGATCGGGTCGCCTGGGATCGGACGACAACACTTCGCAAAGGTGATAAGCACGCCAT
CCGCGCCTTTAATCGGCAGGTGGCCGTGATTCGATTGCGCAACGGTCGGCACCACGGCTTCGCCTTGCTG
CAGATTTTTCGCGACCACTACGCTCATCGCGTTGCCGAGACCGATTTCCGCCAGCAGATCGTCAAGCGTT
GCCAGCTTCATACGATCCAGTTCGCGCTGAATATTTTCCTGCGGAATTTCCGCCAGCTTACGACTGCCGC
CTAAAGCATGGTTAAGCAGACGACGGCCCAGGCTTACGGAGTCATCACGTTTGAGGTTTTTCAGCAACTG
ACGAATTTTAGCGCGCGCTTTAGAGCTGACGACAAAGTTCAGCCAGGCGGCGTTGGGACGCGCGCCCGGC
GCGGTAATAATTTCGACGGTCTGACCGCTGCTAAGCGGCTGCGACAGCGGATAAGGCTGGCGGTCGACAC
GCGCGCCGACGCAGGCGTGGCCGATGTCGGTATGCACTGCATAGGCAAAATCCACCGGCGTAGCGCCAGC
GGGCAGTTCGACAATGCGCCCTTCCGGGGTGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTTACG
CTTTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGTAGCTCCAGCAGGCTCTGCATCCAGCGCTGGG
CGCGGATCTGCGCCGTGGTGCTGGTCTCACCGTGTTCTTTATACGCCCAGTGTGCCGCGACCCCCATTTC
CGCCATCTGATCCATATCTTCGGTACGGATCTGGACTTCAACAGGAACGCCGTGCGGGCCGATCATCGAG
GTGTGCAAAGACTGATAGCCGTTCGCTTTGGGAATGGCTATATAGTCTTTCACCCGTCCCGGACGCGGCT
TATAGAGACTGTGCATCTGGCCGAGTACGCGATAGCAGGTATCGGAGTCATGAACGATCACGCGAAACGC
GTAAATGTCCATGATCGAGTGAAAACGCTGCTCTTTGAGCACCATTTTACAGTAGATCGAGTAAAGATGT
TTTTCGCGACCGCTAACGCGACACGGAATTCCCGCCTCTTGCAAGCGTCCTTCGATTTCAGAGAGGATTT
TTTGGATCATCTCTTTACGGTTGCCGCGCGCCGCTTTTACCACTTCTTTAATGACGCGGTAACGATTGGG
ATACAGCGCTTCAAAACCCAGCTCTTCCAGCTCGGTTTTAATGTGATGAATACCTAAACGGTGCGCCAGA
GGACTGTAGATTTCCAGCGTTTCACGGGCAATACGACGACGTTTATCCGGGCGTAACGAGCCCAGCGTGC
GCATGTTATGGGTACGGTCAGCAAGCTTAATGAGAATGACGCGGATATCCTGCACCATCGCCATAATCAT
TTTGCGAAAGTTTTCGGCCTGCGCCTCTTTCTTATCGCGGAACTTGAGCTTATCAAGTTTCGACACCCCC
TCTACCAGCTCGGCAACGCTTTTACCGAAAAGCTGTTCCATGTCCTGATAGGTGGCGGGGGTATCTTCAA
TCACGTCATGCAGCAGAGCGGCCATCAGCGTTTCGTAGTCGAGTTTCATCTCGGCCAGAATACAGGCCAC
CGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTCTGGCCCTCGTGAGCGTCACGTGCAACG
AGATACGCCTGCCGAAGACGCTTAATCTGGTCTTCCGGCAGGTAGGTTTGAATCAGTTGATTCAGGCTTT
CAAACAGATACA
  • Protein Sequence : Show Sequence 
    >gi|205354656|ref|YP_002228457.1| bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase [Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91]
MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAAL
LHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADR
THNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRK
EMIQKILSEIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVHDSDTCYRVLGQM
HSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGETSTT
AQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGHA
CVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLL
NHALGGSRKLAEIPQENIQRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQQGEAVVPTVAQSNHGHLP
IKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETEQEFITEI
KVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSTFIRLTARDRVHLANIMRKIRVMPDVIKVTR
NRN
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A ppGpp-defective Salmonella Gallinarum, which is attained by mutating the relA and spoT genes, is attenuated in chickens and induces protection from oral challenge with wild type S. Gallinarum (Park et al., 2010).
  • Related Vaccine(s): Salmonella Gallinarum relA/spoT mutant vaccine
52. surA
  • Gene Name : surA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1251610
  • NCBI Protein GI : 16763482
  • Locus Tag : STM0092
  • Genbank Accession : AE006468
  • Protein Accession : NP_459097
  • Taxonomy ID : 700720
  • Gene Starting Position : 104469
  • Gene Ending Position : 105755
  • Gene Strand (Orientation) : -
  • Protein Name : peptidyl-prolyl cis-trans isomerase SurA
  • Protein pI : 7.32
  • Protein Weight : 44868.68
  • Protein Length : 428
  • Protein Note : Chaperone involved in the folding of extracytoplasmic proteins, especially OmpA, OmpF and LamB
  • DNA Sequence : Show Sequence 
    >gi|16763390:104469-105755 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
ATTAGTTACTCAAAATCTTAACGTAAGCGCTGGCGCGCTGTTCTTGCATCCAGGTCGCCGCTTCTTCTGA
GAATTTACGGTTCATCAGCATACGATAAGCGCGATCTTTCTGCGCCGCATCGGTTTTGTCTACCTTACGC
GTATCCAGCAATTCGATCAGATGCCAGCCGAAAGAGGAGTGTACCGGCGCGCTTATTTGGCCTTTATGCA
GCTTCGTTAGCGCGTCGCGGAACGCCGGGTCGAAAATATCTGGCGTAGCCCAACCCAAATCACCGCCCTG
GTTAGCGGAGCCCGGGTCCTGAGAGTACTCTTTCGCCGCAGCGGCAAAGGTGGTTTTACCACTCTTAATG
TCAGCCGCGATTTCTTCCAGCTTCAGGCGCGCCTGCTGATCGTTCATGATCGGCGACGGCTTAAGCAGAA
TGTGACGAGCGTGAACTTCGGTCACGGAGATACTCTGGCTCTGACCGCGCAGGTCATTTACTTTCAGAAT
GTGGAAGCCGACGCCGGAGCGAATCGGGCCGACAATGTCGCCTTTCTTCGCGGTGCTCAGCGCCTGGGCG
AAAATCCCCGGCAGCTCCTGGATACGGCCCCAGCCCATCTGACCGCCTTTTAGCGCCTGCTGGTCGGCAG
AGTAGGTAATCGCCAGTTTGCCGAAATCTGCGCCGTTACGCGCTTCTTCAACAATGCTTTCCGCCTGGCG
CTGCGCGTCGTTAACCTGCTCGGAGGTTGGGTTTTCCGGCAGAGCAATCAGGATATGGCTCAGGTTCAGC
TCGGTGCTGGCATCGTTTTGGGTGCCAATCTGTTTTGCCAGCGCGTCAACTTCTTGCGGCAAAACGGTGA
TACGGCGACGAACCTCATTGTTGCGCACTTCAGAGATAATCATCTCTTTACGAATCTGGTTACGGTAGGT
TGAATAGTTCAGCCCATCGTAAGCCAGACGGCTGCGCATCTGATCCATCGTCATATTGTTTTGTTTGGCG
ATGTTGGCGATGGCCTGATCCAACTGCTCATCCGTGATCTTCACCCCCATCTTCTGACCCATCTGCAGGA
TAATTTGATCCATAATCAAACGTTCCAGGATCTGGTGACGCAGCGTGGCGTCGTCCGGAAGCTGCTGACC
TGCCTGACCCGCGTTGAGTTTGACTGATTGCATTAAGCCATCAACGTCGCTTTCCAGCACGACGCCATTA
TTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATAC
CGAGAAGCAGCGTTTTCCAGTTCTTCA
  • Protein Sequence : Show Sequence 
    >gi|16763482|ref|NP_459097.1| peptidyl-prolyl cis-trans isomerase SurA [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDGLMQSVKLNAGQAGQQLPDDATLRHQI
LERLIMDQIILQMGQKMGVKITDEQLDQAIANIAKQNNMTMDQMRSRLAYDGLNYSTYRNQIRKEMIISE
VRNNEVRRRITVLPQEVDALAKQIGTQNDASTELNLSHILIALPENPTSEQVNDAQRQAESIVEEARNGA
DFGKLAITYSADQQALKGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGQSQSI
SVTEVHARHILLKPSPIMNDQQARLKLEEIAADIKSGKTTFAAAAKEYSQDPGSANQGGDLGWATPDIFD
PAFRDALTKLHKGQISAPVHSSFGWHLIELLDTRKVDKTDAAQKDRAYRMLMNRKFSEEAATWMQEQRAS
AYVKILSN
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A surA mutant (BRD1115) is attenuated in BALB/c mice and induces protection from challenge with wild type S. typhimurium (Sydenham et al., 2000).
  • Related Vaccine(s): Salmonella Typhimurium surA mutant vaccine
53. tolA
  • Gene Name : tolA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium SL1344
  • NCBI Protein GI : 301157338
  • Locus Tag : STM0747
  • Genbank Accession : AE006468
  • Protein Accession : NP_459732
  • Other Database IDs : CDD:181920
    CDD:200776
    UniProtKB/TrEMBL: E1W9P0
  • Taxonomy ID : 216597
  • Gene Starting Position : 814616
  • Gene Ending Position : 815839
  • Gene Strand (Orientation) : +
  • Protein Name : tolA protein
  • Protein pI : 9.79
  • Protein Weight : 40772.04
  • Protein Length : 407
  • Protein Note : cell envelope integrity inner membrane protein TolA; Provisional; PRK09510
  • DNA Sequence : Show Sequence 
    >gi|16763390:814616-815839 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
AGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAACGGGCGATAATTATTTCAGCCGTGCTGCATATC
ATCTTATTTGCAGTGCTGATCTGGAGTTCGTTTGATGAGCATATAGAGGCTTCTGCCGGCGGCGGCGGTG
GTTCCGCTATCGACGCGGTGATGGTCGATCCTGGCGCCGTTGTGCAGCAGTACAACCGTCAGCAGGATCA
ACAGGCCAGCGCCAGACGCGCGGAAGAAGAGCGTAAAAAGCTGCAACAGCAGCAAGCAGAGGAGCTGCAG
CAGAAGCAGGCTGCTGAACAGGAGCGGTTGAAACAACTTGAGAAAGAACGTTTAGCGGCTCAGGAGCAGC
AAAAGCAGGCTGAAGAAGCGGCAAAACTGGCGCAACAGCAGCAGCAACAGGCCGAAGAAGCGGCGAAAGC
GGCGGCGGACGCGAAGAAAAAAGCGGAGGCCGAGGCGGCGAAAGCGGCGGCGGACGCGAAGAAGAAAGCG
GAAGCCGAAGCGGTAAAAGCGGCAGCGGACGCGAAGAAGAAAGCGGAAGCCGAAGCGGCGAAAGCGGCGG
CGGACGCGAAGAAGAAAGCAGAGGCCGAAGCGGCGAAAGCGGCGGCGGAGGCGAAGAAGAAAGCGGAAGC
CGAAGCGGCGAAAGCGGCGGCGGAGGCGAAGAAGAAAGCGGATGCCGAGGCGGCGAAAGCGGCGGCGGAG
GCGAAGAAGAAAGCGGATGCCGCGGCGGCGAAAGCGGCGGCGGAGGCGAAGAAGAAAGCGGATGCCGCGG
CGGCGAAAGCAGCGGCGGACGCTAAGAAGAAAGCGGCTGCCGAAAAAGCGGCCGCCGCAGAAGGCGTCGA
CGATCTGCTTGGCGATCTCAGCTCGGGTAAGAATGCGCCGAAAACCGGCGGCGGCGCGAAAGGGAATGGT
CAGCCATCGAAAGATAGCGGTACATCGGGCGCTAACGGTGGGGCGACAGGCGCTGATATCAGCGCCTACG
CGAAACAGATTCAGGTCGCCATTCAGAGCCGTCTGTACGATGCGAGCCTGTATCAGGGCAAACAATGTGT
CTTGCATATTAGCCTGGCGCCGGATGGCTCATTAAAAAGCATTACGTCTGAGGGCGGCGATCCGGCGCTT
TGTCAGGCGGCGTTAATGGCGGCAAAAACCGCGAAAATTCCTAAACCGCCAAGCCAGGCTGTTTATGAGA
AAATAAAGGATGCCAAACTAGACTTTAAACTGTA
  • Protein Sequence : Show Sequence 
    >gi|301157338|emb|CBW16827.1| tolA protein [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
MSKATEQNDKLKRAIIISAVLHIILFAVLIWSSFDEHIEASAGGGGGSAIDAVMVDPGAVVQQYNRQQDQ
QASARRAEEERKKLQQQQAEELQQKQAAEQERLKQLEKERLAAQEQQKQAEEAAKLAQQQQQQAEEAAKA
AADAKKKAEAEAAKAAADAKKKAEAEAVKAAADAKKKAEAEAAKAAADAKKKAEAEAAKAAAEAKKKAEA
EAAKAAAEAKKKADAEAAKAAAEAKKKADAAAAKAAAEAKKKADAAAAKAAADAKKKAAAEKAAAAEGVD
DLLGDLSSGKNAPKTGGGAKGNGQPSKDSGTSGANGGATGADISAYAKQIQVAIQSRLYDASLYQGKQCV
LHISLAPDGSLKSITSEGGDPALCQAALMAAKTAKIPKPPSQAVYEKIKDAKLDFKL
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A tolA mutant is attenuated in mice and induced significant protection from challenge with wild type S. Typhimurium (Paterson et al., 2009).
  • Related Vaccine(s): Salmonella Typhimurium tolA mutant vaccine
54. trxA
  • Gene Name : trxA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium SL1344
  • NCBI Protein GI : 301160436
  • Locus Tag : STM3915
  • Genbank Accession : AE006468
  • Protein Accession : NP_462806
  • Other Database IDs : CDD:48496
    UniProtKB/TrEMBL: E1WDY2
  • Taxonomy ID : 216597
  • Gene Starting Position : 4124835
  • Gene Ending Position : 4125164
  • Gene Strand (Orientation) : +
  • Protein Name : thioredoxin
  • Protein pI : 4.46
  • Protein Weight : 11310.13
  • Protein Length : 109
  • Protein Note : TRX family; composed of two groups: Group I, which includes proteins that exclusively encode a TRX domain; and Group II, which are composed of fusion proteins of TRX and additional domains. Group I TRX is a small ancient protein that alter the redox...; cd02947
  • DNA Sequence : Show Sequence 
    >gi|16763390:4124835-4125164 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
TATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCT
ATCCTCGTTGATTTCTGGGCAGAGTGGTGCGGGCCGTGTAAAATGATCGCTCCGATTCTGGATGAAATCG
CTGACGAATATCAGGGCAAATTGACCGTTGCCAAACTGAACATTGACCAGAACCCAGGTACTGCGCCTAA
ATATGGCATCCGCGGTATTCCGACTCTGCTGCTGTTTAAAAACGGCGAAGTGGCGGCAACCAAAGTAGGC
GCACTGTCTAAAGGTCAGTTGAAAGAGTTTCTCGACGCCAATCTGGCGTA
  • Protein Sequence : Show Sequence 
    >gi|301160436|emb|CBW19965.1| thioredoxin [Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPK
YGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A trxA mutant is attenuated in mice and induced significant protection from challenge with wild type S. Typhimurium (Peters et al., 2010).
  • Related Vaccine(s): Salmonella Typhimurium trxA mutant vaccine
55. TviA
  • Gene Name : TviA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. CT18
  • NCBI Gene ID : 1250863
  • NCBI Protein GI : 16763124
  • Locus Tag : STY4662
  • Genbank Accession : NC_003198.1
  • Protein Accession : NP_458741.1
  • Taxonomy ID : 28901
  • Gene Starting Position : 4524140
  • Gene Ending Position : 4524679
  • Gene Strand (Orientation) : -
  • Protein Name : Vi polysaccharide biosynthesis protein
  • Protein pI : 9.8
  • Protein Weight : 21113.2
  • Protein Length : 179
  • Protein Note : Similar to Salmonella typhi Vi polysaccharide biosynthesis protein TviA SW:TVIA_SALTI (Q04971) (179 aa) fasta scores: E(): 0, 100.0% id in 179 aa and to Salmonella paratyphi C VipR protein TR:BAA24231 (EMBL: AB006131) (179 aa) fasta scores: E(): 0, 100.0% id in 179 aa
  • DNA Sequence : Show Sequence 
    >GeneID|1250863 [Salmonella enterica subsp. enterica serovar Typhi str. CT18 ] 4524140..4524679
ttacagtaaagtaactgaatccggcaataacagatagcgcggctcacgtactccaaattttactgccaat
tcttttctccagcgatacatagtcgatgatgagttcataaatctatcctgaagctcttccataccacttt
ccgaaaggaaatatttcatctttactatatccttacggttaattctgaaacgataatctctctgctccgt
caagatcttttcaccactttggtgttgactgacatttctgataatatcctggactgatgttgccgcatcg
aaaacagcaataacatttttgcactctgtcaaccaaaaacgtgcaaggggcattagtcgcgaacttgaaa
tgataacgattattttattaatatcactcattcgcgtgaaaaaattcagggcatttaacaggctgtagcg
atttaggttcacaaacaaataatttctgtcatttgttatcagtgacacttcttcaataatgccagcagct
ccaaccccgaaatagatatcattcggaggccagaaatgatgaaacctcat
  • Protein Sequence : Show Sequence 
    >gi|16763124|ref|NP_458741.1| Vi polysaccharide biosynthesis protein [Salmonella enterica subsp. enterica serovar Typhi str. CT18 ]
MRFHHFWPPNDIYFGVGAAGIIEEVSLITNDRNYLFVNLNRYSLLNALNFFTRMSDINKIIVIISSSRLM
PLARFWLTECKNVIAVFDAATSVQDIIRNVSQHQSGEKILTEQRDYRFRINRKDIVKMKYFLSESGMEEL
QDRFMNSSSTMYRWRKELAVKFGVREPRYLLLPDSVTLL
  • Related Vaccine(s): CVD 909
56. TviB
  • Gene Name : TviB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi str. Ty2
  • VO ID : VO_0011269
  • NCBI Gene ID : 1067316
  • NCBI Protein GI : 29144603
  • Locus Tag : t4352
  • Genbank Accession : AE014613
  • Protein Accession : NP_807945
  • Taxonomy ID : 209261
  • Gene Starting Position : 4505426
  • Gene Ending Position : 4506703
  • Gene Strand (Orientation) : -
  • Protein Name : VI polysaccharide biosynthesis UDP-glucose/GDP-mannose dehydrogenase
  • Protein pI : 5.79
  • Protein Weight : 43904.75
  • Protein Length : 425
  • Protein Note : corresponds to STY4661 from Accession AL513382: Salmonella typhi CT18
  • DNA Sequence : Show Sequence 
    >NC_004631.1:4505426-4506703 Salmonella enterica subsp. enterica serovar Typhi Ty2, complete genome
ATTACAATCTCACATCTGACTGCTCAGCCGGAAGAACATACTTCAAATCATAAAGTACATGTTTATCTTT
TCCGAAGCCGCGAATATCCTCACTTCCCATCTGTTTAAATTGCTGATGTCCTACTGCAACAATGATCGCA
TCGTAGTGGCTTGATTTGACTTCCGATACCGGGATAATGCCATACTCTCGTCTTACCTCTTCGGCATCCA
CCCATGGATCAAAAATATCCACTTTACAACTATATTTACCGAGTTCCTTTACCACATCAATAATGCGTGT
ATTTCTGATGTCCGGACAGTTTTCTTTAAAGGTAAAGCCGAGAATCAGCACGCTGGAACCCTCAACGTTA
ATTCCTTTTTTGATCATTGCTTTGATCAACTGCTCGGAGACATAGTTGCCCATGTTGTCGTTCAGGCGGC
GTCCTGCAAGTATGATTTCTGGATAATAGCCAATGCCCTGAGATTTATGTGTCAGATAATAGGGATCTAC
GCCAATACAGTGACCACCGACCAGTCCCGGACGGAATGGCAGGAAATTCCATTTGCTGCCAGCGGCACGT
AGCACGGCTTCAGTATCGATATTTAAACGATTAAAAATAATCGCCAGCTCATTGACCAAGGCAATATTCA
GATCGCGTTGCGTATTTTCAATCACCTTCGCTGCCTCAGCAACTTTGATGCTCTCTGCTTTATATGTACC
TGCGCTGATGATCTGCTGATATACTTCATCGATAAGTTCGGCGATCTGTGCGGTTGAACCGGAGGTGATT
TTCTTGATGTTGGTTAAACGGTGCTTTTTATCACCGGGATTGATCCTTTCCGGGCTATAACCGACATAGA
AATCCTGGTTGAAAGTCATTCCGGACATACGAGCAAGGATCGGCACGCATTCTTCTTCGGTACATCCCGG
ATATACCGTAGATTCATATACCACAATATCTCCCCGATTCAGCACTGTACCAACGGTTTCACTCGCCTTG
ATTAGTGGGGTGAGGTCTGGTTGCTTGTAGGTATTTATCGGCGTCGGGACGGTGATGATGTAAAAATTAC
ATTCTTTGATCTTCTCAATCTCGGAAGTAAATTTCAGATAACGAGCCTCACGTAATTCTTCTTCAGTGGT
TTCCAGATTGACATCCACCCCATTCTTTAATTCAAGAATACGCTTTTTATTAACGTCGAAGCCAACAACC
TGACGAGATTTGCCAAATTCAACTGCCAGAGGAAGCCCAACATATCCCAGCCCAATAATCGCGATTTTTA
CCTCGTCTATACCGAACA
  • Protein Sequence : Show Sequence 
    >NP_807945.1 VI polysaccharide biosynthesis UDP-glucose/GDP-mannose dehydrogenase [Salmonella enterica subsp. enterica serovar Typhi str. Ty2]
MFGIDEVKIAIIGLGYVGLPLAVEFGKSRQVVGFDVNKKRILELKNGVDVNLETTEEELREARYLKFTSE
IEKIKECNFYIITVPTPINTYKQPDLTPLIKASETVGTVLNRGDIVVYESTVYPGCTEEECVPILARMSG
MTFNQDFYVGYSPERINPGDKKHRLTNIKKITSGSTAQIAELIDEVYQQIISAGTYKAESIKVAEAAKVI
ENTQRDLNIALVNELAIIFNRLNIDTEAVLRAAGSKWNFLPFRPGLVGGHCIGVDPYYLTHKSQGIGYYP
EIILAGRRLNDNMGNYVSEQLIKAMIKKGINVEGSSVLILGFTFKENCPDIRNTRIIDVVKELGKYSCKV
DIFDPWVDAEEVRREYGIIPVSEVKSSHYDAIIVAVGHQQFKQMGSEDIRGFGKDKHVLYDLKYVLPAEQ
SDVRL
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : The viaB (tviB) gene coding for the Vi antigen of Salmonella typhi Ty2 was subcloned into expression vector pYA248. The recombinant plasmid was termed SMM202 and transformed into Salmonella typhimurium χ4072, an attenuated ΔcyaΔcrp mutant. Immunization of mice with Vi4072 afforded complete protection against fatal infection with virulent S. typhi Ty2 (Cao et al., 1992).
  • Related Vaccine(s): S. typimurium Vi4072
57. Ty2
  • Gene Name : Ty2
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhi Ty2
  • NCBI Gene ID : 1070398
  • NCBI Protein GI : 29144781
  • Locus Tag : t4545
  • Genbank Accession : NC_004631.1
  • Protein Accession : NP_808123.1
  • Taxonomy ID : 28901
  • Gene Starting Position : 4695660
  • Gene Ending Position : 4698959
  • Gene Strand (Orientation) : -
  • Protein Name : hypothetical protein
  • Protein pI : 7.02
  • Protein Weight : 125222.8
  • Protein Length : 1099
  • Protein Note : corresponds to STY4851 from Accession AL513382: Salmonella typhi CT18
  • DNA Sequence : Show Sequence 
    >GeneID|1070398 [Salmonella enterica subsp. enterica serovar Typhi Ty2 ] 4695660..4698959
taacattacgcaatagacagtattggaaaatggcgttaaaccatcgtgagtcaaaatctctaacctgtct
caagaaaaatacggcgccgggcttattctcaaaaacactttcaaatgttgttttaccgctggcaacagca
gcagcaaatacggggagcaaggtgacggaatagcggtagctggcatcaggggaaatatcgataacaggat
tttgttgcgacatataccaacgtaaaaggcctgggccaccaaattctggccagcgagattcaccatgttc
gcgtagaagttcctgataccactcaaggataatacctttcatcgtcaactcaggaaaaaccggtggtaat
ttcccttgactcaaccataattgcacctcactggcataagtcgggaatgttgtgcccagctgttgataca
ttctgtacagcaaccttatctgcatttcctccggtgcacctttatgagtcaaaaaagcacgaaaacgtgt
ggcggaacgtttaacttctgtaatggataaaaactcccagaaaacgggaaactcactttcaatacggctg
agataatcaatggacatttcaaatttgaacagcgacatcgccaacgcttgtggatgctgaacaagtgccc
gccatacttcaaaggtagccaaaggtaaatacccgaactgatcgtataaattgcgtaagaactgccagcc
cgagtgtgccgggtcgctcgccatttgttccagaactaacataatggtgttgacatctgaacgaggatta
aaaagctgcgttgctttttgtaaagactgtattgctgtcgcgtcgctttgaattaccggctcacctgcaa
taaagcaaggtcgaaatgaggcttcttcacctggtttcggcagcaccagccatgggccattcttttgtat
tatcgaacttagttcaaattcaccaactggtacaccttcgctcattcttgacgtcagcagtacaactttt
ctctccggttcgctgagtaacataatgacgggcgaagggatctggcctgtacgattacttattgagtttg
cgagaagaatctgcctgccacgatcatagtccagactatatttataacgtcggatctgccaggtaaaaga
actaccacctccttttatgcgcatatcaactgtttgatcaattccttcctccagggagagtaaattatca
atatgttcccttaagctatataaagtaagctctaccggacattctcccgcactgtagtgccactcatacc
aggcctgcattccactacgcgaacgtaaacgtagttcaagctggtatcgcgtaggctcaccattttttcc
gaaaagatatgctctggcacccagaagatcgttaatcgttaaatttttcgggagaggtttctcatcttta
tcgaacgcaagacagcctcgtgcaggaaatggtaaccaaataacaatcgggtttgcagtgaggtttggcg
ttatttgtaatgaaattgatgcaggaggaatgccctccgccttcatcattatttctgtactatcgggcaa
ccgcttccgtccaacttcaagtgttttttccttcaagctatacaaacaagggtgctgagtagtaataatg
acagaaccttcgttcgcctgttgtccattttttatttccagactaaaatcattggggagaatgccaattt
tcttacgcagtaacgtttcatcattttcgttacgcaccgaaataaactgtgctcccattttttcttgtac
atcacaattttctataaatgttccgttgaaaaaacgcttgtaatggcgcgttgatagattctcagaatgt
tgagtaattccaggaacaccgaggaagagttcatccggataacttgtccaatttaatcgcttcccctgga
aagagaatccagtatggataatctgatctcttcctatctttatccggtacgtttcatcgcctttaatgat
gatatcctggcgacctttgatggtcagagcatggcatcccaaagccgaaaagccagagtcacaatcctca
tgttctgaagccagacagccatcctttggcagcacaataagtacatggctgccacgtacgctgcaagagg
cttgtccctgtaacagccattcatttttatcagaaacaaaaaccaatggaacatcgccaaccgcaacttc
actgccttctaaaagattggagccgaaaaacatacccccagctctcgccacaataaacaaactgaccgtg
ggatttcttcggtaaaatttaatctcgcgcttacgaacttttatttttgcctgggagttacttagcgttg
catatgcaggtcccagacttgcgatttcgttaccatcttcatagatagcaagctcgaagcgcgttgttga
aggctcaatatcaattgaaaaactcagttcttcaggcagagaaatgagtgcctggagggcttcaggatga
ttttctgaccataaaaactgacagagagtggttttattcttttgaaggcggggttttgattcagtagaag
ctgttcgtaaaagtccattaaggaaactggtacccgtatcatcatcgagagggacagggaaactgtcacg
ccacttaggatgaacacgttcaagttcttttacgggttcggtatgattacttaaatcataaatttgaaca
agagatactaactgttcagccatccgactaataagctcaactgaagtatcctctttgaaaacgactggca
gggatgatttttctaccgctgcatggactaacgcgaacgtggaaatgttagaagatttagcctgatcgta
ttgatttagaatgagagaaaacatcgactgaaaacggctattagattcctttagcaacctgaaaggtaga
ccgccttcactgaataaggagcctaagaaattccgcctttctgtgtcataaaaccgcactggacgccccc
agtaaccatccaggcctttagggataattttccccattttcgaggaagatgctgagatacctatcgtctt
gtaaattggctcccatgcccatccatactctctttcataatctcttcgataccactctgagcagaacaat
gtgaaacaggctgcgtaccctttatcacttttataaccatcgggtacccctatagctcgcagcagccgtt
tcaattcatcatattcttcactcgttgcgtggtattcatataacggacgatggtctggttgcttcaatcc
tctgcgggtgaggaatttagctagccatggggtacactttagaatggatgcgtattcggactgactcaaa
gcaagctcct
  • Protein Sequence : Show Sequence 
    >gi|29144781|ref|NP_808123.1| hypothetical protein [Salmonella enterica subsp. enterica serovar Typhi Ty2 ]
MSQSEYASILKCTPWLAKFLTRRGLKQPDHRPLYEYHATSEEYDELKRLLRAIGVPDGYKSDKGYAACFT
LFCSEWYRRDYEREYGWAWEPIYKTIGISASSSKMGKIIPKGLDGYWGRPVRFYDTERRNFLGSLFSEGG
LPFRLLKESNSRFQSMFSLILNQYDQAKSSNISTFALVHAAVEKSSLPVVFKEDTSVELISRMAEQLVSL
VQIYDLSNHTEPVKELERVHPKWRDSFPVPLDDDTGTSFLNGLLRTASTESKPRLQKNKTTLCQFLWSEN
HPEALQALISLPEELSFSIDIEPSTTRFELAIYEDGNEIASLGPAYATLSNSQAKIKVRKREIKFYRRNP
TVSLFIVARAGGMFFGSNLLEGSEVAVGDVPLVFVSDKNEWLLQGQASCSVRGSHVLIVLPKDGCLASEH
EDCDSGFSALGCHALTIKGRQDIIIKGDETYRIKIGRDQIIHTGFSFQGKRLNWTSYPDELFLGVPGITQ
HSENLSTRHYKRFFNGTFIENCDVQEKMGAQFISVRNENDETLLRKKIGILPNDFSLEIKNGQQANEGSV
IITTQHPCLYSLKEKTLEVGRKRLPDSTEIMMKAEGIPPASISLQITPNLTANPIVIWLPFPARGCLAFD
KDEKPLPKNLTINDLLGARAYLFGKNGEPTRYQLELRLRSRSGMQAWYEWHYSAGECPVELTLYSLREHI
DNLLSLEEGIDQTVDMRIKGGGSSFTWQIRRYKYSLDYDRGRQILLANSISNRTGQIPSPVIMLLSEPER
KVVLLTSRMSEGVPVGEFELSSIIQKNGPWLVLPKPGEEASFRPCFIAGEPVIQSDATAIQSLQKATQLF
NPRSDVNTIMLVLEQMASDPAHSGWQFLRNLYDQFGYLPLATFEVWRALVQHPQALAMSLFKFEMSIDYL
SRIESEFPVFWEFLSITEVKRSATRFRAFLTHKGAPEEMQIRLLYRMYQQLGTTFPTYASEVQLWLSQGK
LPPVFPELTMKGIILEWYQELLREHGESRWPEFGGPGLLRWYMSQQNPVIDISPDASYRYSVTLLPVFAA
AVASGKTTFESVFENKPGAVFFLRQVRDFDSRWFNAIFQYCLLRNVTEK
58. wecA
  • Gene Name : wecA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium
  • NCBI Protein GI : 20140863
  • Other Database IDs : CDD:185074
    CDD:133463
  • Taxonomy ID : 90371
  • Gene Strand (Orientation) : ?
  • Protein Name : Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase
  • Protein Length : 367
  • Protein Note : UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; Undecaprenyl-phosphate GlcNAc-1-phosphate transferase
  • Protein Sequence : Show Sequence 
    >gi|20140863|sp|Q9L6R7.1|WECA_SALTY RecName: Full=Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase; AltName: Full=UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; AltName: Full=Undecaprenyl-phosphate GlcNAc-1-phosphate transferase
MKLLTALSELISIFLFTTIFIFLARKVAIKIGLVDKPNFRKRHQGVIPLVGGISVFAGICFMFGLSDYYI
PHLSLYLICAGVLVFVGAMDDRFDISVKIRAVVQAVIAVVMMVIAKLHLGSLGYIFGPWELVLGPFGYFL
TLFAVWAAINAFNMVDGIDGLLGGLSSVSFAAMGLILWFDGQTSLAMWCFAMIAAILPYIMLNLGILGRR
YKVFMGDAGSTLIGFTVIWLLLETTQGKTHSISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPDRQHI
HHLVMRAGFTSRQAFVLITLAAAILAGVGVTAEYSHFVPEWVMLVLFLLAFFLYGYCIKRAWKVARFIKR
VKRRLRRQRENRPNLTK
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A wecA mutant (DSM645) is attenuated in C57BL/6J mice through i.p. and oral immunization. This mutant also protects against challenge with S. typhimurium strains TML and SL1344 30 days after immunization (Gilbreath et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium wecA mutant vaccine
59. znuA
  • Gene Name : znuA
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253412
  • NCBI Protein GI : 161353595
  • Locus Tag : STM1891
  • Genbank Accession : AE006468
  • Protein Accession : NP_460848
  • 3D structure: PDB ID : 2XY4
  • Taxonomy ID : 700720
  • Gene Starting Position : 1986414
  • Gene Ending Position : 1987358
  • Gene Strand (Orientation) : -
  • Protein Name : high-affinity zinc transporter periplasmic component
  • Protein pI : 5.71
  • Protein Weight : 31911.63
  • Protein Length : 314
  • Protein Note : Also known as yebLinvolved in transport of zinc(II) with ZnuA and C
  • DNA Sequence : Show Sequence 
    >gi|16763390:1986414-1987358 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
GTTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGGCTTAAAAACGCTGAATAGCTTGTTTT
ACCCAGTTTAATGTTCGTCCCGAGGGGGTCCAGTGTTCCCATTCGAACGGATGTCCCTCTCGCCACGGCT
TCCACGACCGCTGGCCTGAATTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTCAACCAACTGTGTTC
TTATTTCATGTAAACGCTGCGCGCCAGGTTGTATCTCAGGGTTCACGGTAAAGTGACCGAGCGGGGTGAG
TCCGTAGTGTTTTTCGTAGTAACCGTAGGCGTCATGAAAAACGAAATACCCTTTCCCCTTGAGCGGCGCG
AGCTCGTTACCGACCTGTTTATCGGTTGCGGCTAATTGTGCCTCAAAATCCTTCAGGTTGGCGTCGAGTT
TGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTTCATGGATTGCAACCGCTGTAGCCCGCGCTATCTC
TGGGGAGAGCCAAAGATGCATGTTATATTCGCCGTGATGGTGATGTACGTCACCTTTTTCCTCATCGGCG
CCCGTATGCGCATGTTCATCCTCATCATCATCCGCGCCCTTCATGAGTAACGGTTTTACATCGGCAAGTT
GCGCAATGGTAACCTGCTTATTATCAGGGATATTCCTGACCGACTTCTCCATAAAGGCTTCCATTTCCGG
GCCAACCCAGACGACTAAGTCCGCGCCCTGTAAGCGTTTTACGTCTGATGGACGCAATGAATAATCATGC
TCGGAAGCCCCATCCGGAAGTAATACTTGCGTATCCGTAACGCCATCAGCAATGGCGGAAGCGATGAACC
CAAGCGGTTTAAGCGAAGCGACAACGGCGGCGTCGGCAGCCTGGGTTGCACTCCCCCAAAGCGCGGCGGA
TAATGCTGCGAAAAGAAGCGTATTTTTCTGTAACA
  • Protein Sequence : Show Sequence 
    >gi|161353595|ref|NP_460848.2| high-affinity zinc transporter periplasmic component [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MLQKNTLLFAALSAALWGSATQAADAAVVASLKPLGFIASAIADGVTDTQVLLPDGASEHDYSLRPSDVK
RLQGADLVVWVGPEMEAFMEKSVRNIPDNKQVTIAQLADVKPLLMKGADDDEDEHAHTGADEEKGDVHHH
HGEYNMHLWLSPEIARATAVAIHEKLVELMPQSRAKLDANLKDFEAQLAATDKQVGNELAPLKGKGYFVF
HDAYGYYEKHYGLTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVEAVARGTSVRMG
TLDPLGTNIKLGKTSYSAFLSQLANQYASCLKGD
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A znuABC mutant is attenuated in mice and induced significant protection against a challenge infection in the large intestine with wild type Salmonella typhimurium (Pesciaroli et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium znuABC mutant vaccine
60. znuB
  • Gene Name : znuB
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253414
  • NCBI Protein GI : 16765235
  • Locus Tag : STM1893
  • Genbank Accession : AE006468
  • Protein Accession : NP_460850
  • Taxonomy ID : 700720
  • Gene Starting Position : 1988189
  • Gene Ending Position : 1988974
  • Gene Strand (Orientation) : +
  • Protein Name : high-affinity zinc transporter membrane component
  • Protein pI : 8.72
  • Protein Weight : 24998.23
  • Protein Length : 261
  • Protein Note : Also known as yebIinvolved in transport of zinc(II) with ZnuA and C
  • DNA Sequence : Show Sequence 
    >gi|16763390:1988189-1988974 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
CATGATTGAATTATTACTGCCCGGCTGGCTGGCCGGGATGATGCTGGCCTGCGCCGCTGGTCCTTTGGGC
TCATTTGTGGTCTGGCGTCGGATGTCCTATTTTGGCGATACGCTGGCTCACGCGTCGCTTCTGGGCGTGG
CGTTTGGCCTGTTGCTGGACGTTAACCCCTTTTATGCGGTAATCGCCGTTACGCTGCTGCTGGCCGCCGG
TCTGGTATGGCTGGAGAAACGCCCTCACCTCGCGATTGATACCTTACTGGGCATTATGGCGCACAGCGCC
TTATCTTTAGGACTGGTCGTGGTCAGCCTGATGTCCAACGTCCGCGTTGATTTAATGGCTTATCTGTTTG
GCGATTTGCTGGCCGTCACGCCGGAAGATCTTATCTCGATCGCTATTGGGGTCGTTATTGTGCTGGCCAT
TCTCTTCTGGCAGTGGCGCAACTTGCTGTCAATGACTATCAGTCCGGATCTTGCCTTTGTCGATGGCGTG
AAGTTACAGCGCGTGAAGCTGCTATTGATGCTGGTAACGGCGTTAACTATTGGCGTGGCGATGAAGTTTG
TCGGAGCGCTGATCATTACGTCGCTGCTGATTATCCCCGCCGCAACCGCGCGTCGCTTTGCCCGTACGCC
GGAGCAGATGGCGGGCGTCGCCGTTGGTGTAGGTATGATAGCGGTTACAGGTGGACTGACCTTCTCAGCC
TTTTATGATACCCCGGCAGGGCCATCCGTAGTGCTGTGTGCCGCGCTGCTGTTTATCTTCAGTATGATGA
AAAAGCAGGCAAGCTA
  • Protein Sequence : Show Sequence 
    >gi|16765235|ref|NP_460850.1| high-affinity zinc transporter membrane component [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MIELLLPGWLAGMMLACAAGPLGSFVVWRRMSYFGDTLAHASLLGVAFGLLLDVNPFYAVIAVTLLLAAG
LVWLEKRPHLAIDTLLGIMAHSALSLGLVVVSLMSNVRVDLMAYLFGDLLAVTPEDLISIAIGVVIVLAI
LFWQWRNLLSMTISPDLAFVDGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTP
EQMAGVAVGVGMIAVTGGLTFSAFYDTPAGPSVVLCAALLFIFSMMKKQAS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A znuABC mutant is attenuated in mice and induced significant protection against a challenge infection in the large intestine with wild type Salmonella typhimurium (Pesciaroli et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium znuABC mutant vaccine
61. znuC
  • Gene Name : znuC
  • Sequence Strain (Species/Organism) : Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  • NCBI Gene ID : 1253413
  • NCBI Protein GI : 39546331
  • Locus Tag : STM1892.S
  • Genbank Accession : AE006468
  • Protein Accession : NP_460849
  • Taxonomy ID : 700720
  • Gene Starting Position : 1987437
  • Gene Ending Position : 1988192
  • Gene Strand (Orientation) : +
  • Protein Name : high-affinity zinc transporter ATPase
  • Protein pI : 8.96
  • Protein Weight : 25186.17
  • Protein Length : 251
  • Protein Note : Also known as yebMinvolved in transport of zinc(II) with ZnuA and C
  • DNA Sequence : Show Sequence 
    >gi|16763390:1987437-1988192 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
CATGACAAGTTTAGTTTCACTGGAAAACGTCTCGGTCTCATTTGGTCAACGCCGCGTCCTCTCTGACGTG
TCGCTTGAATTGAGCCCCGGAAAAATTTTAACGCTTCTCGGCCCTAACGGCGCGGGGAAGTCAACGCTTG
TACGCGTGGTTTTAGGACTGGTAGCCCCTGATGAAGGGGTGATCAAGCACAACGGGCAACTCCGTATCGG
CTATGTCCCGCAAAAGCTCTATCTCGATACCACGCTTCCGCTGACGGTAAACCGATTTTTACGTTTACGT
CCCGGCACGCAAAAAACGGATATTCTCCCGGCGCTTAAACGCGTTCAGGCCGGACATTTGATTGACGCGC
CCATGCAAAAACTCTCCGGCGGTGAGACACAGCGCGTACTGCTGGCGCGTGCTCTGCTTAACAGGCCTCA
GTTGCTGGTACTTGATGAGCCGACGCAAGGCGTGGATGTCAACGGCCAGGTCGCGTTATACGACCTTATC
GATCAGCTGCGTCGCGAACTCGATTGCGCCGTGCTGATGGTGTCACATGACCTACATCTGGTCATGGCAA
AGACGGATGAAGTGTTATGCCTGAACCATCATATTTGCTGTTCCGGCGCGCCGGAAGTGGTGTCTATGCA
TCCGGAATTTATCTCTATGTTTGGCCCGCGAGGCGCGGAGCAATTAGGGATTTATCGCCATCATCATAAT
CATCGCCACGATTTACAGGGTCGTATTGTACTGCGCCGGGGAAATGGTCACTCATG
  • Protein Sequence : Show Sequence 
    >gi|39546331|ref|NP_460849.2| high-affinity zinc transporter ATPase [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MTSLVSLENVSVSFGQRRVLSDVSLELSPGKILTLLGPNGAGKSTLVRVVLGLVAPDEGVIKHNGQLRIG
YVPQKLYLDTTLPLTVNRFLRLRPGTQKTDILPALKRVQAGHLIDAPMQKLSGGETQRVLLARALLNRPQ
LLVLDEPTQGVDVNGQVALYDLIDQLRRELDCAVLMVSHDLHLVMAKTDEVLCLNHHICCSGAPEVVSMH
PEFISMFGPRGAEQLGIYRHHHNHRHDLQGRIVLRRGNGHS
  • Molecule Role : Virmugen
  • Molecule Role Annotation : A znuABC mutant is attenuated in mice and induced significant protection against a challenge infection in the large intestine with wild type Salmonella typhimurium (Pesciaroli et al., 2011).
  • Related Vaccine(s): Salmonella Typhimurium znuABC mutant vaccine
1. human IgA
  • Gene Name : human IgA
  • Sequence Strain (Species/Organism) : Homo sapiens
  • NCBI Protein GI : 2632187
  • Other Database IDs : CDD:209398
    CDD:197704
  • Taxonomy ID : 9606
  • Gene Strand (Orientation) : ?
  • Protein Name : IgA
  • Protein Length : 131
  • Protein Note : Immunoglobulin domain; cl11960
  • Protein Sequence : Show Sequence 
    >gi|2632187|emb|CAA10818.1| IgA [Homo sapiens]
LQQWGAGLLKPSETLSLTCAVYGGSFSGYYWSWIRQPPGKGLEWIGEINHSGSTNYNPSLKSRVTISVDT
SKNQFSLKLSSVTAADTAVYYCASRVGYCSSTSCYTFDYWGQGTLVTVSSASPTSPKVFPL
  • Molecule Role : Vaximmutor
  • Additional Molecule Role : Vaximmutor
  • Related Vaccine(s): Aro-deleted S. Typhi , CVD 908-htrA
2. Ifng (Interferon gamma)
  • Gene Name : Ifng (Interferon gamma)
  • Sequence Strain (Species/Organism) : Mouse
  • NCBI Gene ID : 15978
  • NCBI Protein GI : 33468859
  • Genbank Accession : NM_008337
  • Protein Accession : NP_032363.1
  • Other Database IDs : MGI:107656; UniProt: P01580
  • Taxonomy ID : 10090
  • Gene Strand (Orientation) : ?
  • DNA Sequence : Show Sequence 
    >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA
ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA
GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA
GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT
AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC
AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT
GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC
AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC
GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA
AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG
AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC
AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT
TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC
TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT
ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG
GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC
AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC
TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA
AGTATACCTTTGCAACC
  • Protein Sequence : Show Sequence 
    >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus]
MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI
ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ
LLPESSLRKRKRSRC
  • Molecule Role Annotation : IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria.
  • Additional Molecule Role : Vaximmutor
  • Additional Molecule Role Annotation : The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes (Wang et al., 2009).
  • Related Vaccine(s): Salmonella Typhimurium hfq mutant vaccine , Salmonella Typhimurium ruvB mutant vaccine
3. IgA
  • Gene Name : IgA
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 238447
  • Genbank Accession : AC160982
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 8607
  • Gene Ending Position : 12639
  • Gene Strand (Orientation) : -
  • Protein Name : immunoglobulin heavy constant alpha
  • Protein Note : Also known as IgA; Igh-2
  • DNA Sequence : Show Sequence 
    >gi|121699722:8607-12639 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
AGTACTGGGGGACCTCTTTGCTGCCAAACGGGCCTCGAACAGTTGTAACAGTGAGTGCTGTGCTGTAGAA
CAAGCTCAGTAGGAAGAGGGTGAGGAAGGTCACAGTGGTGGGCCACAGGCTGGCACCTGGGGCCTCTTCC
TCCAGGATGTCTTCTGACTGGTCCAGTAGCACATAGGAAAGTGGCTCTTGACGTTCTGTAAGACATAGAT
GACCTTCTAGATCCAAACCCATGGGGATGGGGGCTCAGACTTAAGGTTTCTGCTTCTATGCCTCCTTGGG
CTTTGGGGACAGTGGTGGTAGGGGTCCCTTATTCCCGGGAAGGGACTTGTTCAGGGATAATGAGGAGGCA
GGGTCCCAGGTAAGGGACTGGAATCCCAGCAGTTCTTGAGATGGCCCCAAGACCCATCTCTCTCTCTTAG
GGAAACCTTAATTAGAGACCTGTCATGGTAAGAGAGTTGTTTCTGTCAAACTCTGAGAAGAGTTTGAACC
CAAGGCTCCTTAGGTCTCAGTCCTTTCTAGATATTTAGCAATACCTCCCTAAACCCCAGTTCTTAGGCTA
ATCTTGGCTTGGGTACTAGGGTTGTGTAAGATCAGACACCTTCAAACAGATACAGGCCAATGTGATTTCT
GGGCCAAAGTTAGACTACCAAGGCCTGGACATGTGGTCAGAGGACTCTGTTGCATTCTTAAGAGCTGTGA
CTGGGGGTTAAGCAACAGTAAAGACCCCCAGTAGAGCCAACCCTGCTGTCCTCAGGTTCTCTGCGCACAG
AGTTTCATTTTAAGTGGCAGTAGGAAATGTGTACTGATAGTCAACCACTCGTATTTCTGTCACTGCAGCC
ATCCATCAATCCATCCATCATCCCCATCGTTCATCCTTGTAGCCAGCCATCCATTACCCTCCAGCCGCTC
ACTCATCATCTTTCCCGACTATCGTGATTCTCCAGCTTCTCTGCCCCCTCTCCCTGTGGGGATTCAGTCT
TTCACCTATTGACTCCTCCTTACTAGGATTCTCTGTCATGGGCCAGGCCTTGGATTCCATGGGTGGCTAT
GGTACTTTTCTGAGTCAGGTTTCCCAAGCTGGTCTGTTGCTCTTGCTATTCTCAGGGAACTGGACTTTCA
AGCTGGAGAGTATCCAGGGCATGCATGGGCCTTTACTCCACTCTTGCTCTGAGAGCAGAGGGAAGTTGCT
AGAAACCAGCAACTCCTCCCCCCCCCCCCCGAAGTTCTGAAGGCCTGTGAGACAGGAGCAGGGCCCCCAC
TGCCCTCTTGTGGACACCTGGGCTCCAAGTATACCTGGTTGTAGAACTTTGTGTTCTAGGGGTGCCCATG
GAGGAGGCCGAGGCCTGTGTGGGAGTGGTCTCCATGGCCTGCTCCTGCTTCTTCCCAGATTTGCCTCTTA
TCCTGCTTCTGGTTTGTGATACTTGGGCTCAGGATGGGAAGGGAGACTTAGGTTAGAAAGGGGGCACCTG
GAGGGGCCTATGCCCTTGTCCATTGTGTGTAGTTGTCAGTGTCGGGCCTGGAAATTCTCCCGGGGGCTTG
GAGGGGACTCCCCTTGCTTTCCAGAAGCTGGGTGTGGGCTGTGAACTTCATCTTCCTCCAGGGTTCAGGT
CTGTGTGAGTGTGCAAATGTGAGTGTCTGTGCATGTATGCCCATGTGCATGTATGCAGAACAGGGTGTAT
GCCCATGTGCATGTGTGAAGAGCAGGGTGCTGCTGAGGGTTGTCTGTGCCATGCTATATGTCTTTCTCAT
GAGTCAGTGGTCACCCTGAATGCATGTTTCCATTCAGGGATGGCCTACTGAGGGTGCGTGGCATCTTCTT
CCCAGTGCCCCTGTGTTCATCCCTTCATTCTCATACCATCATCTCTATTGTGACCCCCACACGGCACCAG
CCTTGATGGTGCTACCCTTTTGCTCCATCTATCCCTGTCTTGTTTGGGTTAGAAGCTGCGGTCTAATAAA
CAGCCACACCACCCACCACTCAGAGTACTTGTGCAGCAACCCACTGCCCTGGGGACTCTCCCATGAGCTT
TGCCTTCTTGGCTGAGTCTGCTCGCCTTGGATTTCATCCCCAGTTTATTCTTACTTCCCATTCACTCATT
GCACATTCACAGGGCAGGTGCACACACTCTCATGTTTCCACCAACATGAACACACATAGATATATGCATG
CTCATAGGTATGCACAGGCACACACTTGCTCACAGGGACATAGATATAGTGTGTCCACATATGTACTTAT
ACCCATATACATACCTATAAACCATAGGTGCACACAAACTCACTCCTCTCTTGCTGCACACAGGTACACA
CATGAAGGCACATGGACCCATCCATAAGCACTCACCAATACATAGACATGCATACACATATACTCTAGGC
ATGAAGGGTCTTCAAAGTCTCCGAGGCCTTACAAGCTTAGGTGGTGGTGTTTCTCCCTCCCTGGGCCCTG
ACCCCTCCCTGTGTTCTGTAGACCCTAAGGATGATGGACAGGCACTGGATGGAAGTGCAGGGATACTTTG
GATGAGCACAGAGTTTATTTCAGGAGTAGGGACAGGCAGGGTGGCTCAGTAGCAGATGCCATCTCCCTCT
GACATGATCACAGACACGCTGACATTGGTGGGTTTACCCGACAGACGGTCGATGGTCTTCTGGGTGAAGT
TCATGGGCAAGGCCTCGTGGCCCACCATGCAGGAGTACTGGTCACCCTGTTTCCAGAGTTCAGCTGATAC
ACGCAACACGCTTGTCACCAGGTAGGTGGTGGCTCCCTCGCCTGGCTCCTTTAGGGGCTCAAACACTAGG
TAGCTTTCTGGGGACAGCTCCTCATTTCCATGCAGCCATCGCACCAGCACTTCTTTAGGGTTGAAAGCTC
GCACCAGGCATGTCAGGGACACGAGCTCATTCAGGGCCAGCTCCTCCGACGGCGGCGGTAGCAGGTGGAC
CTGGGGTGGGAAGGTGTTCACTGGAAGGTAGAAAGAAGAGTTATGGCTCAGACAAGGAGAGCAAGACCCC
TCTGCCCTTCCCTTCTGGGCAGTTCCTCAGATAGCTCCAATTTCCCTCTGTGATATAAGGACAGGAGCAG
TTAGTTGAGCATCTGTATTATAGGAAGAAAGCAGGCATGTGAGCAGGGAACGTCATACAATGTCCCGGGT
ATGCATCTGGGCTCACCTGTGATTTTGGCAATTGTGCCAGTTAAGGTGTCAGACTCAGGATGGGTAACTG
TGCACTTGAATGATGCGCCACTGTTCCAGCGCTCAGCACAGCCAGGCAGGACGCTGGACACACTGTAGCA
GCCGCAGGAATTCTGCACAGCTTTCTTCTGCACTGCATCCTTCCCAGTGGAGGGCTCCCAGGTGAAGACA
GCTCCCTCAGGATTTCTCAGGCCATTCAGAGTACATGTGAGGCTGGCATCTGAACCCAGGAGCAGGTCCT
CAAGAGCTGGCCGCTGCAGTGACAGGCTGGGATGGCAGGAAGGAGGACAAGGAGGACAAGGAGGAGGAGG
ACCTGTAACAGAGAAGTCCTAGCAAATCAGTATATTCTCCTTTTCTCCTTTGCTGTCCCCTTTTCTGGAT
GTTCTGAGGCCTCAGTTCCTTATGGCCTGTTTACGCTTCCCTTCCCCCACCCCCATGGGAGGTTTCTGAG
GGTCCCTGTGAGGGTGTGCCTCAGAGGGATATATGGATCTAGATAAGGTAGAACTTATCCCACCCCCAGC
TGACCCCCTAACGTTCTTTACCAGAGCACTTCACATCCAATTCTTGGACGGCGTTAGAGTCATGTTGCAC
GGAACATTTCACGGATTCTCCTTCTGGGCACTCGACAGCTGGCAGGGTCAACTGGCTGCTCATGGTGTAC
CCTCCCCCAGAGGCCAGGGCAGGTGGGAAGTTTACGGTGGTTATATCCTTCCCACTCTTTCCCCAGGTCA
CATTCATCGTGCCGGAAGGGAAGTAATCGTGAATCAGGCAGCCGATTATCACTGGGTCACTTGACAGAGC
TCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGAC
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhimurium asd mutant vaccine , Salmonella Typhimurium asd/rfc mutant vaccine , Salmonella Typhimurium clpP mutant vaccine , Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine , Salmonella Typhimurium hfq mutant vaccine , Salmonella Typhimurium poxA mutant vaccine
4. IgA Fc receptor
  • Gene Name : IgA Fc receptor
  • Sequence Strain (Species/Organism) : Gallus gallus
  • NCBI Gene ID : 771853
  • Taxonomy ID : 9031
  • Chromosome No : 26
  • Gene Starting Position : 2406653
  • Gene Ending Position : 2408482
  • Gene Strand (Orientation) : -
  • Protein Name : Fc receptor, IgA, IgM, high affinity
  • DNA Sequence : Show Sequence 
    >gi|118135660:2406653-2408482 Gallus gallus chromosome 26, reference assembly (based on Gallus_gallus-2.1)
ATTAATACTTGGCTAACATTCCTACTTCAGCTATCAGGGACAGAAATGATGAGGGTGAAGAGTGAATTGC
ACCTGTGTCTCTGCATGCTGAGGTTCCTAGAACTGCAAGATAACCTTCAGAGCAAAGCACATCTCCATGC
TGCATGTGTTGTGCTCAGGATGCCATGTGCTGGTGTTTTCTATCATAAATTGTCCATGATATTTTCAAAC
AAACTTGAGCAGAAAGCTCAAATGTTTCTTGTAAAGCCACTACATTTATGCATGGGACAAAGGAGAGAAT
TTGAAGGGGGTATCCATGAAGGGATAGGGGGAAGAGTGATTAACAGAAGGAAAAGGGTTGATTTGTGTTT
CCAGCCAGCCATCGCCTACCCGTCTCGATGCGCAGTTTCATGGAGGTCGTGTGCTCCCTGTGCCTGTTCC
TGGCCACACACCAGTACCACCCCGCGTCCTCCTCCCGCAGGTGGCTCATCACCACGGTGTAGGTCCCACT
GTCCTGCTCACTGTTGCTGATCCATATGCGCCCTTTGTAGGACTCGTGCACAAACCCGTCTGTGTCTACC
AGCAGGGAGCAGCTGGCCTCCTGCCACCTGCACAGGTACTTGGTGGTGTAGCTGCCCACGGGGTTGTGGT
GGCACCTAAGGGACAGTGAGCCCCCCAGCCTGCCATGCAGAAACTTCAACCTCCGGGGCTGGGCAGGGAC
TGCAGGTAGAAGAAATCATGGTTATGTTTGATGTAGCTGCAATGAGCCAATGGGGCACAGCAGCCCCCAA
AACCCCCTCCTACCTGTGGTCACCTGCAGGGCCACCAGCTGCCAGCTGTCCCAGTCAGCTGGGCTGCCCA
TCCCACACTTGTACAGCCCTGCATCGTCCAACCTTAAATCATTTATCAGCACTTTGAATGCTCCAGAGCT
CTCCTGGGGGGTAATACAGATCCGTCCCTCGTAGCTCTTCCCCACATAGCCATCGGTGTCAGCAATGAGG
ATGCACCTGCTTCTCCCCACTCTGCACCATAGCCTCCTGGTGCCACCCCAGCTGTCCCCGGGTGGGCAGG
GGATGGTGATGGAGCCACGGAGCTCCCCGAGGTGCAGCTCAATTGGCCTCTGAGTGCCCACATCTGCAAA
GACAGAGCAGGTGAGTGCCACCACTGTGCCTGGTTGGGTGTCACCTCCTTTTGCATTGGTGCATTGGTCA
CATCAGTGGGTTTTATGCCCACGACTGCTTAAAGCAAAGGGTAGCAAAGGGGACCTGACACCCTCCCTGT
CCCATCACTGTACCTGCTGACACTGTCAGGTTCAGGCTGACGTAGAGGTCCCTGTTGGTGGGGCCAATGC
CACAGCGGTAGGTCCCTGTGTCACTTGTCTTCAGCTGTGTCATTGTAACCATGAAAGTGCCATGCTGGGG
GACGTCCTCCAGGGCCACTCGGCCCATGTGGTCTTTTGAGATGAAGTTGGTGGTGGAGATGATGGTGTAG
CAAATCCCATCTCTGTCCCTTTTACACCAAAACTTTCTGTCGTATTTGTTGGCAGGCGTGATGGAGTAGA
AGCACTGGTGGGTGATGGACCCTCCAACTGCGCCTGTCAAAAACCTGGGCCCATACAAGGTGCTTGAGGC
TGCCTCTTTGGGGAAGGAGGGAAGAAGCACATTAATCAGAGACGTGGACACCCCTGTGGATGGGGAATCT
TCCCCAACCCAAATGGGGTGCCACAGGCACAGGTCCACCAGCCCTGGCTGTCCTGCCCCATAGTGATGGG
GAGCAGCAAACCTTTGGTGCCTGCAGAGTTTTCTGGTGACTCACCTTGCAGCAAAGACACAAAGAGGAGG
AGAACCTCCA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Gallinarum relA/spoT mutant vaccine
5. IgG
  • Gene Name : IgG
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16059
  • Genbank Accession : AF010213
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 881411
  • Gene Ending Position : 914690
  • Gene Strand (Orientation) : +
  • Protein Name : immunoglobulin heavy chain (V7183 family)
  • Protein Note : Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)
  • DNA Sequence : Show Sequence 
    >gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
ACAAGCACAGTGCAAATTTCTGTCACCCTG
  • Molecule Role : Vaximmutor
  • Additional Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella enterica fliC/guaB mutant vaccine , Salmonella Enteritidis guaBA/clpP mutant vaccine , Salmonella Typhi phoP mutant vaccine , Salmonella Typhimurium asd mutant vaccine , Salmonella Typhimurium asd/rfc mutant vaccine , Salmonella Typhimurium clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP mutant vaccine , Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine , Salmonella Typhimurium hfq mutant vaccine , Salmonella Typhimurium poxA mutant vaccine , Salmonella Typhimurium ruvB mutant vaccine
6. IgG Fc fragment
  • Gene Name : IgG Fc fragment
  • Sequence Strain (Species/Organism) : Gallus gallus
  • NCBI Gene ID : 424904
  • Taxonomy ID : 9031
  • Chromosome No : 9
  • Gene Starting Position : 12628992
  • Gene Ending Position : 12700391
  • Gene Strand (Orientation) : -
  • Protein Name : Fc fragment of IgG binding protein
  • DNA Sequence : Show Sequence 
    >gi|358485503:12628992-12700391 Gallus gallus breed Red Jungle fowl, inbred line UCD001 chromosome 9, Gallus_gallus-4.0, whole genome shotgun sequence
TGCAATTATTTATTTATTATTCTGAAAGTGTTAGAAATATTATTAAAATACAAGAGCAGAAAGTAATACG
TATGAATTGGGGGGAAAAAAACAACACATACAGACACAGAAAAACACACTAACTACAGGTATGTACTTTG
GGGTTCATCTTGGTCAAATGCAGCGTTCACTACGTAGCCATAATTGTGTTCCCGTACTCTGTCACCTGAT
GAATCCTCAGACTCGTGCAAAGATGAATTCCGTGCATTGATGTTGGCATCTGCCTTTCTCCTCCTTCTGT
ACACAAACAAACAAACAAACACCAACAACCATCTTTAATTTCTTATCTGGTCAAACGGTGGTGTGAGTGT
GTGTGTCAGATTCCAAAGAATTCTAAGTTTTGTGGTAGTGGTGCATATGCATTATGTAATAGGAATGGCA
CATCTTCATTCCTGTAGAAGAAAGCTTTCCCTCGTGTTCCTTAAATGATATCTTGAACGCTGTACTTCCT
TTCTGATTCTGCAGGTGTGGGAATAGAGGAGTTTGGGGGTGTACAGAAGCCAAAATGAGTGTCTTGCCCT
TGCTGAATGTAGCAAAGCTGGGAATAGTCTTACCTGAACAGATACACTGCAGAGGTGATGAGAATGACAA
CGGCAACCACTGCTACTGTAACTCCAACAGCGATGGCAACTGTATTGCTCTGAGAACTCTCTGGGATACT
TGTGTCATCTGAAGGAGAAAGGAGCCCGGTCACTATAAGCTAAGAGCACTGAGAACAGAAGATTCTCCTC
AATTCCAAGTGTGAAACTGCACTGTGTGGAATCTGAACAGGAAGCAGACGTGCCTGTCTGCTTGGTTTAT
ACTGTGTTGTCTGACTTAACTGGCTTTCTGGAATGGTGTCAGACCAAGGTCCTGTATGCCCTGCAGAGCC
CTGCTGTCTTGCCTTCTAATACCTCTGTGCTGAGGACCTGCCCCCTTCTTTCTGTTTGAACATACCAGTA
CCAACATGACCACCCAGCTTGTGGTACAAGGCCCAGTTTTTACTTGAAGGAATAGTGCCACTGAATTGGT
TGGAAAGTGTAGTGGAATATTTCTAGTGTCAGCCTCCACTCTGCCCATGACTAAAATACAATCTGCCCTT
GAAAAGATGTGATGAGGTCCCAGACTGATCTGACACCCCCTCCCAGTGGGTTACTTATTGATTCACAGCA
TGGCAATTAAAAGCCAGCTCAGTCTTCTCCATAATGACACATTTTTTTTTTTTTATAGTGGTGTGTGTAA
TTCAGGCAGAGAAATCCAGAGTAGTTTTATTACCTATCCTTTCAATATTGCATCTTTGTCCCATGTACAG
CTCTGTACATTCACAGTAGTAGTTATCAGAGGTGTCATTGGTTGTTTCAGAGCAAATTCCATCATTCTGG
CAAGGACTTGGCATACACGGTCCACCTAACAAAAAGGAAGGAGAAACACATCAGAATCTCATGTAATCCA
GCCCTGAGAGAACTACTCTTGGTTAGATTACACTGCTACTGCTGGACGTGCCCTGTGTTTTAAGGCCTGT
TATTTATTTTGTACTTGATAACAGGAGTATGGGAAAGAAACAAAGTTCTCCAGTAAGCTTATAAAGAAAG
CTTATTATTTCTTCTGTTCAGACTGTTGCAACCCCAGACCCAGACACATCCTATAAGCAGTAACTCAAGA
CTTCTTAGCAGCGAAGGAAAACACGATAGGTTTAGTGGTTCACTTACTCCTGTAGCACCCACGAGTGTAG
TTTGCAATGTTACAGATTTCATCAGAACCACACAGTATGTTAGAGCAGGTGACAGTGGAGCTCTCTGTGC
ACTGGCATTTCTGGGAGCAGTCATCAGTGGTGAACACCTCTCCAATCTGGAAGATAGCAAGTGAATGTTA
AAAGATATCCAAATCAGGCAGGGAAACGTGAATGGAACACTGGAGCCCCATGCTACTCCGATTCTTCCTG
ATGAATGACAACATGGCAGATCTCTCTTTTTTGTACTTTGAATACAGAAAGCAAGCAAGCAGCTCATTAA
CCCATCTCCTTGAAACTCAAGGAATTTTGATGAAAATTTGAGGTGTGAGATCCCAGACCCAGGTTGGGTA
TATGGACAACCAACAATATCTAACTGTTGCAGGAGATAAAGTGGCAAATGCATCATATTGTGCCTCCAAG
ACGATGTGCAAAGGTGCCATGTGTCATTTCTCTGGCAAGCCCCATTTGTTTAGAGTGTCTACCTCCTCCC
ATAACACCACCTTTACTTGCTTTAGGCCTTCTCTGTATCAAACCTCCAGTGCTGAATTGACTACAAAGCT
TTTTATCCCACAGCTGGAAGGGTGGAATCTCTGGATTCCTCAGAATCCAGCCTTGCCGGAGGCTCTCTTC
ATGCTGCAGCCAGCATTACAGACTCTCAGCCCCTCCTGTGCCACTCTTCACTGAGAGGAGAGGGACCATG
GTGACCAGTAGAATTTTTCTATAACTGGCGTATTGTAAATTTTAGAGCCTATTGTGGCTGTTTACTGTAA
TAAGGGTGAAGAATTAACAAGGAGGTCAGAGTGTAATCATATGCTTCCCCCAAGTGCCTGCAGAGTATTC
TGACAGGCAGAGTTTCAGAGGCAGACTGACTCATGTATTCTCTCCTCCCTTCCTCCAGAGACCAGAATAT
TATTAAATAAATTGCTCTTTGTTTCTAATGCAAAGTTACATGCTGAGGAGAACAAAGAGATTATCAAAAT
AATTAGCACAAGAAAATATTGTTTAGCACTGCGGGAGCTGTCTGGCCTGCTGAGACACTGTACCTCATAA
TATTTGTCAAGGTATGTGCAGCCACATTCTCTGTAAGGCACACATTCAGAGTCACTAAGAACATAGCCAG
GGAGACATTCACAGCCTTCAACACAAGGCGATTCACACTCAGAGGGGCTGGTCATGTCGCTGCAGGATGC
TGGGCAAGCAGTCATGCATGAGTTGTATTTGCTGTTGGGTGGACACGACATCTCTGTAAGAATAAGGAGA
GTCCAGTTATAACAGGTTGCTGCTACACATTGCCTTTTCAGAGCTGTTTTGTAAGGGCAAAATGATGAAC
ACACATACATAGAAATGTTCACATGTATATAATGGTAATGAAGTTATAAGTGAATAACTTGAAAATAAGG
AAATGCATTCTCAAAGCTATGCATATGCAGTGCTCTGAGTCCAGGTGTTCACTAATATCAAATTACTTCT
CACACATACAGAGTAGCTATCACTGAAAGCATCAGAGTAGTTTCACAGCTCTGAGAAGTGCGTTTCATCA
GAAATACAAAATCCTCTGCCCATGGGTGATGCAGGGTAAGTATGGCCTGGATAAACCTCTCAGCTTGGGA
AAGAAAAGTTAGGAAAAAGAGGTTTGAGATTATTGTAAATCCTATTTTCACATTTTTCAGTCTGAAAGAC
GAGTTGGGAATGGAGACATTTTATTTTTCTCACCGAACTTCTCATGGCAGGGGAGCTGACCGCTCTGCTC
CCCTGAGCAGCTTTCTAAGGTGGAGTCTCATACAAAACATCAGCATAAGACTCAGAGGCAGTGACTGAAG
AGCAAGTGTGCGTGCTAAACTATGAACGATGATTGAAGTGTGAATCAAAGTTTGTAAGACCTAAGAAGCT
TTGACATACAGCTTCTAGCAAAAACCTCTAATCCCATATTTTGAGAGTCCTTTAGGCAGAAACCACACTT
TCAATTTCTTCCTGTACTTACCACAAGCTGTCTGTTGCCTCCAGCCTTCCATATTGACTCCTTGCTCCTG
ACAAGCCAGCACGTACTCCTTCAGATGGCGACATAACGTGTCAGCATCATCTTCTACACACATATCGAAG
ATGCAGTTCCTATGTACAACATGAGAAAGCATTCATGCACTATACCTTTTATTTTTTGTTTGCTGCTGCT
ACCTTCTCAACTTTGCCGATCATCTGAATAGCAGTGGCCTGATTTTCAGAGGCAGTGAGAACTGAGGTGA
ATGGGATCTGCAAGTATACAGTAACTCAGAAAAATACAGCAGCCACTAGGAAAGTAAAACAGGCTGCCCA
ACGAAATTCTAGAGTAAGGAGAAAAGTGGGCTTACATTAAGAATCAGGATATAGACCCTGAAGTCAGTGG
TCCATTTGTTTGGTTTAAGGTACTTCCACATTCCTCTCGCAGCTAAGAGAGGCTTTGAAGTGAGCACCAC
CTATCTTTGTGCATTACTTACAACTCTGAGAGTAAAGAAATCTGGTAGCTGCTTGGCCATTCTTTGACTG
ATAATGAATGAATGATAAACCTAAGAATTTATCAACAGGTTTCATATGGTTCAAAACCAGGTGTGTATGA
ACTGTGTACTTAGAAGGCCAGATGAACAGAAGGAACTTTCTTGCCAGTGCCATGTGAGTTATCCTTCACT
AAACTGGTTTTTTGCACCGCTCTGTGAAAGACTGAAGAACTGTCACTGACAGCTTTAGTCTCTGCAGCTC
TGACTTGAATACTGCATTTTACAAAACTACAATTGCTAAGAAGAACCTAATAGAGGCAGGTACTGACTGA
ATTATCACTGAATGCGAATGAGAACTTTGAGCACAGTGGAAATTCCAGCCTACAGATATAATGTTCAATG
CTGCTGCTTTAGGTAAAGAGGAAAGCTACTTTCTAGAGATAACAGAAAAGTGAAACTAAATAGAGAGAAA
AACATATCTTGTTTTAAAAAACAAACAAACAAATATTGGGAACTAAACATATAACTAAGAGAAGCTCTGG
GCCCAGTTCTCCAAAGACACTTACTGTCCTCTGCTCCTAGAAACTGAAGACGTACAGTTCTTACCAGAGA
CAAGCTCCAAAATTGTTTTGGAAGTGCGGAGAAAAATTGCAGATTGCAGCAGACACTTACGTGGAATAGG
GCTCTGGTGGGACTGCAGAATGACAATTTGCAAAAGGACCATCCAATGCAGTCAGAATTTGACACTTAGA
AGTGCTGTTTTCTTGCATCAGCTTATTTTCACTGCAGCCTTGATAGAGCCCTGTATCTGGTTCCTCATCA
GGCTCATCTTCCACAATGGTTTCTCGCCTGGAAATGAAATCATTATGAAACCACCAGATCTCTGTACATC
CAGCACTCATAGCACTAATTTGCAAGTAACAGCATTTCTGCCTTTTGATAGAAGTGTCAGCTTATTTCTC
TCTCAATATTTATACTGAATGTGAAATGAGTATATAAAGCAAATCAAAGATGTACTAGAATAATTCCTAC
CATTTCAGAGATATATGAGTTTTTAAATAAAAAACAATAGATGTTGTTATCTCCATGGACATTCTATATG
ACATAGGTTTCATCCAGGCCTCCTTCACCTAATCCATTCTATAATAATTGAATGCTAGGCAGCTGGAGTA
GACCACCATTTATTGGAAGTATGTTCACTTTCACTGAAGAGCATGCCTGAAGTGTCAGAATTCTTCTGCA
GGTATAGATTCCAGAGCCTCCCTATGCTCAACATTAGCCTACATGCTGCTTTCTACTATCCTGCTGGACA
GGTTTTTGCAGCCAGATTATATCCTGAAATGAATACAATTCTATTCATGTACTGGAAAAAAACAAATAAT
ACAGTAATTTCTGTTGGTGGTTAAGCTACTCAAAAACCTTTCAAAGCAAATTGAAAAGGTGGAAGGAGAG
AAGAAAACTTAGTTCCTTGCTTCAGTGTTCTGTATGGACTAAATAGGGCAGTTTGGCCTGATCACAAATA
TCACACTGATTTTTATTCAAAAGAATGTCCCAGGTTTTTTTTTTTTTTTTTTTTTTTGTACTAGCAAAAA
ACACAATGTAGTCATGAAAGTAAGGAAGTTAGACACTTGAAAAATCTCTTCTGTGAGGAACTGTGAATTC
ATCCTGATGGATTTTGAAGGTGCCTTCATGTTCCAAAGAGAAGGATGACACTGAATCAGGATCTGCCTGT
CCATCCAGCAAATGATGCATTTAATCTTTGCCAGGACCCATGGGCTTGGCAGATACCTTATTTATCCCTG
TGCTCTAGCTGTTGTAATGGCTGTGACTCCAAAGGAAGCTGAACTAGAGCAATGCACTGAGAGCTCAGTC
CTGTTTTGGCAAGATGGAAAAGCACTGATATTTCACCTTAGTGTTGAGCAGGAAAAGTCCCCACATGCAG
ACTAAGGGAAGAAGTAACAGAAACCTGACTGCATAGTTAGACCTGAATATTAACTATGGCTTAATTTCCT
CACTTCCAGTTGCTGTGTTTGCTAGGGATATTAAGTAGAAAATTAGGTACCTGAATCGAGAGGTTCTTCT
TTTCACAGGAACCTTCCAGCTCTCACCAAATGCATTCACATTCTTCAGCCAAACACCATCTGGGTTTGTG
AAGTCATTTCTAGGTCTCCCGTCAAAGTCACCACACAAACCTTCTACTCTGTTGTTGTAGGTGTTGGCCA
GCTTTATATCTAGGAAAGTTCAAAGGAAAAAAAATAGCCATTACAAAGAAATATTTTCTTCCGAGTATAT
GGAGGAGGTGGAAAGAAGTGTGAAGTACTGAATTCCACTATTCAGACTACAGTTGGTAGTGTGATGAAGG
ATTTGAAATCATCAGAAATACCATGGCTGCATTACATTGTAAATCAGGGGTAGCTCCACTTTGGATAGAG
GAGGTAATGTGTTTTCAGCTTACATGAATGAGTTCTGTATGCATGATATGAAAGTCCTGATGAGAAACAC
TAAAGAATGGGATTATCTGGAACACGCTTGAGAGGAAGCATGTAAAAGAATGCACTCTCAGTTGTTCAGC
TTGAGGACCTCAGTCCTGTAACTGGGTTAAGCTGGGAAAGGGGCAGATCCAGACTTTTCCCCATCGTCCT
GATCAGCAGCTTAAAGTAATTTGCTTTTGTTTCCATCACTCAGGCCTTACCTAACATTAAAAGTAGTAGA
ACAGTCTGACTCCACATACAATTTCTCCTAAATTCAGATGGATCCAGATAGAGAAATGCCAACTTCCATG
AGAAATAATTTTTGTTTCAGTTTTCTGGAAGTTCATGTCACAAAGAACCACAGACTTAAAGGGTGAAAAT
TTCTAATTTTATCTTGGGTTTAGACCTACTTTGTAATGATAACATTTTTTGACATGGTGCATAAATATTC
CTGCTGATTGTTTTAGTATCACTTACCTGCATTTTGATCACCATCGTAGCTCAGGTATAAGCCAAAATCT
GTCTCCAGGTAAATTCTTGTTGTCCTCTGATATATACGAATACCATCATGGGGACGAACAGGGGCTCTCA
CCCTTACACCATCCAACTAAAAGGGAAAGAATAAGTTAATACTTATAATACAAATTCGTATGTGGCTTTG
ATGCAGGTAAATGCTACAAAAGAGCATTCCTCTTTTCTGGTTTTATGATAAATATATAAGATATTTTAAC
CAGGTAAAAAATACAGTATTCCACTTTTAAAGTAACAAAATCATATATTAAGGAAAGTGATCTGGACACA
GTGTTCTTACATCATTATCATTCCTGAGAGATTTCCAGTACTGAACAAAAATGCATGTATGAGCCCAAGT
ATGCTATTATGGACCAACAAAGAGGACAGAAACTGAGTTTTGCCAGCATAATTCACACTTGTCCCTACCA
CTGATCATATTTTATGTGGCTTTTTGGTAAGGTTTGGATATTTGGTATTTTATTCCTAGAAGTCCATTTA
TTTATTCATTCTAACTAGAATCTATCTTTCACAGCTTTAGAAAAAAAGAACACAACTGAAATCACACTTT
TGCTTGCCCCACAGTACTGCTGTAATAACACTGTTAGCTTTAATACTCTTTAGAACAATCCTTTAATTTA
TCTGTTTTACTACAAAGGAGAACCTTTGTCTGTGCTCTGCAGTTAGTGAGAGACTTACCAGTATCTGCTT
GCCTTGCCTGAATCGCACTTCGTGATTGTAAACGTTGATGATAATCTCTTTCAAGTAAGTAATGCGTCGG
TTTAGACGAAGAGGATAGTTCACGCCTTCGACGCTGAACTGTGTCAGAGTATCAGGTAGGTCAGGGATGG
TCTGAGTCAGAATATACGTGTATTTCCCTTGGAAGTGGTGCACCAAACCATCAAATGTGATATAATGTGG
ATCGCCTATGATCCGGCACTTGCCAAAACCTAAAGAAAAAGAGTTTAATTGCCTTCCAACCTGAATGCAC
GTACCAAATCTTATGCATAAATCTGCTTAATACAGTTTAGATATAAATAATTGCTGCTAACATGCAATCC
TCTCAAAACAAAGACGGGTGCTCAGGCATCACCCTAGCAAATGGGTACTCAGACAGAAAGCCAACCAGAG
AAACTTATTCTTCTGCTGAGAACCAGTGGGAACCAATAGAACTGAAGCACGGCATTAGGGTTTATTGCTT
TTATAGGCACTAAGCTGCACACATCAGTCCTCTGCAGAGGAATGACTGCCACACAGGACGGCTTGCAGTG
GGCTTGTAAAGATGTTGGTTTCATAATCCTCAGCAATATGATCATCTGATCATCAATACAGTAGGACCTA
TGCCATCCTATTCTTGAAAATTCATGTGTAGAGGTGTTAGGCAACAAGCTACCAATTAAACTGTCCAATT
TACAATGAGAACATGGGATTAACCATAACATAGCAGGGCTGACACCTCAATGTAAAAGCTTAGCCAAAGC
AAGTACTGGATGAAAAGCACCAAGATTGGCTATTAAGTATCTGTTGTGATCTGCTCTGTCCTGTTTTGCC
TTTATTTTTCATATTTTCAAATCAATATGCAGTGAAGAAAATAAAAAAGAATGCCAAAGGATTAATAGAT
GTATATATTAGACTTTTATTTTTAGTAAATGCAGTGGATATATATATATATAATCTGCCAAGTTTCTAGA
TGTACATAGATTTTCAAACTCTTGTTCTAATTCTCTCTACAAATTCCCTGTCTTGTACTGTAACTTTCTA
TTCTGATAAATGAGGCCTTTTTTTGTACTTAATAATGTTTCATCAGTAGTCCTGTCAGACCTGTCTTTTC
ATGTCGTAAGGGTTGGAAGTGACATGGAATAAAGAATGGACATTCACATCAATCCTCCTTCTCTAGTCTT
CATGGAATAGCAGATGAAGATCTGCCTGCACATACCTGTTGGATTGCACTTGTATTTTCCACTTTTGATC
AAGCAGGTTTCTTTAGAATCACATCCGTAACTCTGACATCTGATGACACCATTCTGCTGACATTGACATC
TTTCGGTGCAGTGTGGAGTAATCCAGGTCTCCCCAGCCTGGACACAAATTGTGGTATGTTACCAAGGTGC
TGAGCAATAGAAAGTCAGTAGCAAAACGAGTGGAGTCTGGCTAGGTGCATCCTGCTGGCCATTCCACAAC
TGCTCAATCCAGGTGTAAACTTATCAAGAAGCTGGGGGGGGGGGGGGGCAATCTCAGTCCATTTATCTTC
CACAAGGGAATTGGAACACATAACAGGAATAGGATATACCACCCAATTAACGCGGTCAACTTTCCAGTAT
GTCTCAATTTTGGCTTCAAGGTGCAAACGACCACATTCGTGCATCCTCTACCAACTTAAGCCCATAGCTT
TTGCTAAGCAAATGAAATGTATTTAAAATGGTAGACCCTGAAGACTTTTCTTTCTCATTCTGTTGGACAC
AGAACCAATTTCCTGCAAGTTTTGCAGTCAGAAATTTACCCTACAATGGACTACTTACACTGTAGTAATT
GTTGTCATCATCCCTGCAGCCACAGTTGCTGAGAGGAACACACTTGTCATTACTGAGCACATAATTATCA
TCACACTCACAGCCCTCTGTGCATGATTCTGTCTTCTCACAAAGGGATGAGGCAAAAATGTCATTGCATG
TTGATGGGCAGGCAGAGGCACAGGCTGTGTAATGGCTGTGGGATGGACAGGGCAATGCTGTGTGGGTGAA
GAGAATAGAAGTAGTGTTATTAAACACGTTAAAACAAATGTTCTTGCCTGATATCTTCAACTTGGCCTGT
AAAATAGTGGTGTAGTTCTGCCTTCTTATAGATCATTTTGATCATCAAACAAAAACTTTTTCTTCTTTTC
TTTCTTTTTTTCATAATTAGATGGTAGCTTTGTTGAATGGATGGAGTGTGGCACATACAATGGAAATTAA
TCCAAGAACTTGGCCCCTAATAAGCAACTGTCTGAATTGTCAGCAAGTGGCTTTGATCCCTGTGTTCAGT
TCAGTTTCCCATTTGGTTTTTTACTTCTGGTTATACTACGGTTATTATAGTTGGACTTGATGATCTTGAA
GATCTATTCCAACATAAATGATGCTGTGATTCTATTATTTCTCCATTTTAGACTCACTGCAGTATCTCAA
GACATTCATTTGGCTACCAAAACCATTCTAGCTCCACACTATCACCCACAACTCATCTAGTTGTTATCAC
TGGGTTTAGTTCAGCTTGCAAACAGTTCAGCAACATAGTCAAGTCATAAAATCATAACAACAGAATTGTT
TATTTTGAATATTTTGTCTTAATTATCTTTTATATAACTTGTCTACCTAATACTGCTCATCTGTCTTATG
CTGTCAGCCATCCAATTTTCTGTTTTTTTTCACTTTCTTTTGCACTTACGACAAAATGTGCTATTCCTCC
ACGTGATGGTGATGCCTTGATTCCTGCAGGTGTCCACATAGAACTGAACTATGTCACAGAGGGCAGACTT
CATGCCATCATATCGACACATGTCATAGGTGCAGATCTTTATGAAGTCTTCGGGATTCACCAGATCATGA
CATGCTTTGAATTTGTCTGATTTTAGGACGTTGCACTGACTTTCAATGATTGGTTTATTCTCATCAGTGC
AAGGAGGAACATCATCTTCTCTTGAGTCTGACAAACAACTGCAAGAAAGCACAGAAACAGCTGCACTTGA
GTTTCAAAGGCAAATGTGAGACTGAGACAGAAACTGGAATGTTTGTTGCCACTGAAAGGTACTAAATAAA
TAGTGCTGGAGACTGCAGTCTTACTTCTGTCTATGGCTGGAAGAGTAGCAGCAGTAGATGTGCATGACAA
TCACTATGCTGTTTCTTGACCTTGGTTATGCTCACTGAACCTGACCCTGACAAGAGGACTGATTTCCCAT
CTTGACCTTGGACCTGCCTCATCTCTGCGAAGTTGTGTGGTGACCTGGACTCTTTGCTGAGTCTGGCTGC
CACCCTCGGGCACACACACTGCTTGCCTTCTGTGGACACTCTGCATGGGCTTTGGCTGGTGAACCCATGG
TTGTACAAGCTGTGTTACCATGCTAGGCTCTCTGTACCTTAGGGAACAGCTGGCCATCACTGTTCCTGAA
CTCCATTCTCCTGTTCTAAGTATGTTTTTCAGTGTAAAATAAATGTAAGCTGTGTCCACACTGTGGACTA
CTCCAAAGCCTAATTCCCCACGTGTGTGTGTCCACAGTAAAATGGCTATGGTTTCCATCTGGCCTGTATG
GTGATTTCTGGTGGTGATGGCTTTTTTTCTTTTGAGCTTTTAACTGAAGAAGACTAAAATCTCATACATA
CTGTACATTACAAAGTCATAAAGTAGACAAGGCAATTAGAGGAAGACCGTTCCAAAGAAAGATGGAAGGT
ATGAAGTAATGCTTACCCTGCATCACTGTCGTCTTCTACTTTCCAGCTATTTCCAAACTGTGTGACACTG
ACAAGTGTACCATTGGGCAAGGACAGATCATCTTTATAATTGTCGTTGAAGTTACCACACATGCCTTGTA
CCTGCAAGCAAACAAGAACTATACTCTAAAAACAATCTGCTCTGCACCAGCTTCCCCAGTAAGTTCTTAA
TCATTGCCTCTGGAATGATAAGGTTGAAACACTAATGACAATCAATCTTATACTAAATATTTCAGACATA
CTGATTGCACAGTAGAAAATAAATAGACACTATGATACCCTAAGACTTCAAACCATGTAATTCTATAAGC
TGGATGTATTCCCCTAGCTGTGTCATTTTCTGTCAGTTCTCATTTGGAACACAAGGAAATTGGTGCGAAG
AGAAGAAATAGGGAAAAAATGACTTGTGTTACAATACAGCTGTGAAGAAAGATGCAATAGCAAAATACAA
GAACAAAGAAAATGAAAATAAATACACAAGCAGTGGTTTTGAAAGAATACCAATATGTCTACAAATTAAA
TCTTACCTTTGAGAAGTAAGATTGTGGGAGAGTTATTTCCAGGTGGTCATCGCCATCATATTTGACTATC
ACACCAAAGTCTGTTTCCACTACTAGGTAATTGCCAATATTTCCAATGGACACCCCCCGCACCCGGTTCT
CCACTGGAGTGTAGACCCTCTCACTGTTCAGCTGGAAACAAAAATCATTTGTCAGTATGGATGATCTCCC
ATGAAAGCTTTTCTGCTTGAATCTACATTCCTTGGAGTGCTGAAGATTACTCTGACAGTTTTAGATTCAT
CACACTTTCACTATTACATATTCCACCTACAGGTTGCAAAAATCAGCTTTTCAGCTGAGGTGAGCTGGGC
AGGGCTCTGGTGATTATAGCTATAGGTGACTGGCCTGACAACAGTTACTGAAGTGGGGAGGTACATTTTT
TAGTTCAGAAAACAGTTCTCAGATCTGGAGGCTCTGGGCTCCACCTCCAAGAAATAGCAAATAGAACAAT
GAAACGAGAAGACAAGCCTTGGGAAATAGTCAGGAATTATATTTCCCCACAACATCAACTTAAGGGAGGT
GCCTGTAATTATTAAGGATATGCATACACGTTTCTTATGAAACATAGATGTCCTTTTTCCTACAGCTACT
GTAGTGAGCTAATTTTAAGATAATTCTTGCTGGAATAAATAGCGCCAACACCTACCAGGATTTCTTTGCT
TTTCCGAAGGGTGATTCGTGTACCATACACATCAATGTAGACTTCTTTCAGGTATGTGGCCCCTCTTAGC
CCTCGGTCTTCGTTCTTGCCAAGGATGGTTATTGGGTTGACATTGTAGCTGACAGACTTAACCAAAGTGT
AGGTGCAGGTACCCACAAATGTGTACATCAATCTATCAAAGGTGAAATAGTGGGGATCTCCTGCTACCTG
ACACGTTACTTGGCCTGAAAGTGAAAAAAAGAAAGATCTAAGGACTTTGGCCTGGATTTTACCATGGACT
CAACTGACCATAGACATTTGTATTAGTGCAGCAACTTAATCCTCATCACTATTACAAGGAGAAACTTTCA
TAGAAGTATTTCAGGTTCATGTCAATGGCATTTACCTGGTAATGAGAAATTTCCAGTAGGTATCTCCTAA
ATTTAATTAAAAATTACATACAAACTGGCAACTTGGCAACAAATTAACCTGTTTATCTGCTTCTATATGC
TGCTGTATCCATAATGCATGCTAATTAAGAGTAATCCAGAGCTTTCCTATGTAGCAATTTGAGAATACCT
AAATATATGAGTAAATATAGGTATCAATATGAATGTAAATAAATATGTATCTATATACATAATTATGTAA
GAAACTTGCCAACAAACACTCTCTCAGAAATGGCTCTCAGTCCCAAATTTAGAACTTGCAGCACTGGTGA
TTTGTTTCTTGAATTTGTATGATCAAGACAAAAAGAATCCAAGGGACCTTACAGGCTCTTAGGCAGCATC
AATTTTTGCAGATATCAATTACAGAAGAAGGGAATCAGTGACCACAAGATAGATCATCATGTCTTCTTGT
ATCTTACCACTGGCAGCAGTTAGTTGGAATTATCATTGCCATCACATATGAGAATGTATTTATGAGAAAG
TTAGAGAGAGAATAAGAATCTAATCATAGAATTGACACTGAGAAGAATTTTCTGGGAGGACTTACCACTG
GAATGACAGCCCAGCACACCATCCTTAACACTGCATTCCTCTCGTACTCCACACTCCCAGGATGAACAAG
CAATCATGTTATTAGCATTGCAAGTGCAGCGTTCAGTGCAGGAATAATTGGTGAACCAGCTTTCACCCAG
CTGAGGGGAAAAATATGTAGAATTATATACTATAAGATAAATAGGATAGTAATGCCATAAGGGACAATCA
AATTACTAGTATTTGAATGTGCACAATATGTGATCATTTACTCACTTATGACAACAGAACTCAGTGATCA
AGAAGGCTGGAAAAGTGAATCTTTTTTGCTATTACTAACAGTGTAAATAGCAATTGTGATAGTGTTTTTT
CTCACAATGATAGCAATTAAGACATGTTGTTCGTTCTTTTTTTTCTTTTTCTTTAAATATTGTCTATGGT
AACTTGCCTCATGATAGTGGTTTTTGTCATCAACACAACCACAGTTGCTTTCTGGCACACATGTGTCTCC
ACTCAGAACATAGCCATCCTTACAGAAGCAACCCTCCACTGGCAATGAACTACAGGTACTAGCAGCCGAG
GAGTTCACACAAGTAGAGGGACACCTAGTACCACAGCCTTTGTAGATGCTGCCTCCAGGACAGGATATTG
CTGGAGGAAAAAGGAAAAAATAAGATCACTAAAAATGACAACATTTTTATAAAAATTATACCGATGCTTT
CATTTCTACTGTTCGCTTCTGAAAATTAGGAAAACTGTTGTTGGAGAGTCTGACACTGGAGACTACAGAT
TACCAAGAGTCTTCTTGAACAGTGAGATGGTGGGTGGCATCTGGCAGTGGTGGGCAAGACCAGTAGGCCA
CCCTGCACCTATCTCACATAGGAAGTCAAACATTGCAGTCTTGCCTTGAGTGTAGGGCTGACATGATCAT
ACAGACCTAAATGTGCTTCATTCTGTAATCCTTCTGGAGGTTTAAAGACATCCAGATGTGCTGGAAACTC
TAATCCTTCTGGTTTTGGCTGCCTTTAGTATCTAAACAGGATTACAGCAGCAGTGCAGAGCTGTCTGGTA
GTTTTTCTGAAAACAGCTTAGCTATGTTCAAAGCCCTGAAGATCACTAGTAGGTGACAGAGGGCAGTAGG
GAGGCCATGGAAAAGAGGAGCATGGAAAAGATAAAACCCCTAAGTATGTACAATGCAGCTATGTCATGAC
AGATAAAAGTTGCTGAGGCACTGCTTTCAAGTTTCATCAGAGTCAGAACCATGTATGTTCTGTCTTCCCA
CCTTTGGGCAATTTAAGACAGAAAAGAGAGAATGGGGAGGAGCTGGTTAGAAAGCTGGGGCTGGCCAGGG
AAGATATAAGGCTGCAGGAGGGATTACATCTTTACAAAGCCAGTTTGCATTACATGGATCTAAACTTCAT
TTTCAGCAGTATAGTGGACAATAGTAAGTTTCATAAAGGTTTAGTTTGAACCACAACTCTCAAGGCTACT
CATATCCAGGGATTAAATAAAGCATTTCACTCCTCTGAGCTCTGTTATTTCCTGACTACTCAAGTCACTC
AATTAAGGGAGTAGAAGCATCTCTTCACATATCAGATGGTATGTCTCTGTTACTGCAGTTACTATCTGAA
CACCGGTCACATTTAAAAATGGACCTAAAAATCAGATTCTTTTCCATTGAGACCCCCTGAGAAAATACAT
TGTGCTCTCACACCCCTCAGGGAAAATACTGTATTTTACAAAAGTCTTGAATCATTTGTCTATTCCTGTG
GTTTGAAAAAAACACCAAATAGTATTTTTGTGACGATCCGTGAAAAATCACCACATGTGAGTCTCATTCA
CTTCCATAACTACAGCAGGATCTCTTACTCCTGTCAGACAATTACAGCCAACACCACCAGTCATCTATCC
TTTTCAGATTCCTAATTGATTTTTTATTTGAACAACTGATGGAAGGAGCATTTGAGAGACAGTCCTACTA
ATGTGACTGAGCAATCATCTTCTCTAACACCATAACTAAGCTGTAGGTATTTAGGTCCTGGAATATGGCC
TTAGACTGTAGAGCCTGGAAGTCCGTACTTTTAATCTGAGTTGGGGGCCTCAAAGCTATTTTCTTCTTCT
GTCTTCTCTTTACAGACAAAAGGCCAGTGCTCTGCCCTAGGGCTGACTTATGTGTGCTTTTAATCAAAGG
CCATTCTAAAGTCATTCTTTTACCAAGCAGCTCCTGTCATAAAATTATTCCAAACATAATTAGTATGGAG
ATAAATGAGAATACTTCTGTAAACTTCCTAGAAACACTTGTTTCTTAAAATGTCTTACTAAACTAATTTT
TCTCCAAAGGCCATCAATGAATATTGAGAGCCTACAAATATCAGCTATGTTCGTATTGCGAATGGTGGAT
TTTCAGCAAAGCAACGTAATAGTTACTCTCCCAGTGCAGAAGCTCACAAGAGATACAACTGACTATAATC
CAAACTTTCCAATAAAATAATAGGAAATTATAAATATTTGAAGAATTAGACTAAAATGTATCCAAAAGTT
CATGAAAATTATTGAATTATGACTCCCGACATACCAAAAGACTAAACTCGAAGCACTACATATATTGAAA
CATCATACATGAAAAAGATAGAGGAGAATGTGAATAATTTAAAGTTTAAATACAGAATAAATATTTATAA
ACCACAGCACTCACGGCAAAGAGTAGCATTTCTCCACTCTATACATATTCCAGCATTGATACATGACTCG
GCGTAGGCCTGCAGTCCATAGCATAAGGATGTTGCCTGTCCCTCAGTGAAACACATGTCATAAACACAAT
TATTGAAGAAATTTTCGGGAGGCACTTTGTTGTGGCAATCCTTGAAGATTCCTGTGTAAACAAAGAAAGA
TACATAGAGGACAGAAAAGAAATGGGGAATTTATCACCATCAGCATCTATTGTCTGTCATGGCAAATGTA
TTGAGTGCTCCAGGCTGACTTTGTTTAGTAAGTTAAATCAACACTTGAAAATGAGCACATACAATAAAAC
CTGGCCCTCTGGATCAGGCTTGTTTCTTCTCCCAGTGTGATTTACACAACACAGCATACATAAATTAGGA
CCTGCTGGCTAGGCAAACCAATAATTACAAGCAACTTCAGGGAATCTCTAATGCAGGAAACATCTACAGA
ATTTCAGATGAAGTATCTATAATTTATGCAATGACAGTGCCCTCTGAACTATTTCATTTAGAAAGCAAAA
TGCAAGTCACTACAAATCATAGAGCAGCTATGGTTTCTGGTGATCATTTTCACATAATCTACATGAACAT
CTTGGCACTGTCTCATCAGTTGACATATCTTGACCTATGAATTTAATTTGGCCGTTGGCACTGGATATTT
TATACACATTCAGTGTAAGGTCAGAACCATGGGTAAAAAGACAGCTACAAGCATTCTACCTGTTGGGTCT
GTGATCATGCCACATGCTGTATTCTTCTTTGCTTCACTCTCCAGCACAGCATCACATTGGTCTTCTGTTC
CGACAGAGCACCTTGGGAAAGCAAAAAAATGAAGTGTTTATATACATCTCTTATTACCAGATCTTTTGTG
GAATTCTGACGCACAATAACCCCACATAACAGTGCATTTTGTTACAGTCAGAAAAATTTATCTAGCTTTT
TTGAAAGGATGATGTGAGCCCATTTCTGAATGAATGCCCTTTTCAATGCCTTTTGGGCCAATCAAAAGAC
TGTTCTTGCTGAAAAAAAAAAAAGAGCTATGGGATCCTGTAAGTTAATGATTTCCAGTCAGGGATCTGGA
CAGCAATCACTCCTGACTCCCATAAACAGTTCTTGCTTTGGAAAACAAGGCTTAAAGGATGCAACCCCTC
TTCTCATTCCCTCTTTAATAAGAAGAGGGAGCTCCGTACTTCTAGTGGGTAATTGGAAGATGCAGAGTAG
TACCTGGATGACAAAGCCAGTGAATGGATAGATATTTGATTTCATAGAGCTTGATTAGCAAAAGTGATTA
GATTCCTGTACATACATGGTGTTATTCTCAGGTACAAGCCAGCTTTGTCCAAGTTCATTGGAATTACTTG
CAGTGTCACCGTTGGGCTTTATGTTGTCATCATTTGGATCACCATTATAATTACCTGTAAGGGTAAAAAA
AAAAAAAAAAAGGATTTATAGGATACATGAAGTTCACACCTGTAGAACAAACATAGATTTCAGGCTATAA
AGGAAAATGCAATGAACTAGTGAGAGTTTATATCCAATGCCAATGTGCACCGGGCAAAATAAGAAGAGTA
CTTGTATTTGGATCTCATCTGTAGTGTTGGACTAAAAATCAAATATTATTGTCATTTGCATCATTACATC
CTTGGGTAGGTTTCCTGAATTGAAAGTGAGAAACAACCACACAGAGGTAATTTAACTAGAATATTTCAGA
TGATCAAGATCAAAGAATAATCAGAAAATCAGAAACTCATAATACAAGCTACTCTTACAATTTCTACAAT
CCCATTTCCATCCTGAAGTATGTTTCATGTAATCAACTGGATTACAGAATGTTGTTCCAACTAAACCTGC
TCAGATTCAGGCATTGCCAACGTGACTGATGAAAAATGACTGATTGCTACTAGCACTTCACTCTCATTAG
CTACAAAAACCATCTGTGACTCTCAGTTACTTTGTGTTCTTACTGTAAAAGAACTTCTTACTGTAAAAGA
ATGTGGTAGAATGTGCCCTTTGTGTCTGACCTGTTCTTTCTCAAAGGACTCTCAAAGGGTTCTATCTTTA
GTGTTGTATGTTAATGTTTTGTGGCATTTGAATTTTTGAAACTACATTTTTTTTTAATCTTATTCATCAA
GTCTATCACCTGAGGATCATCCATACTTGATTCCTTCATTGCTTTGTTTTACTCTAGACATTACCTATCT
AAGGTAGAATAGAATCATTACTCACTCTAATTTACACTGATAAGTGAGTTTTGTGCTTTTTTTTTTTTTT
AATCCAGCTAAAAATATTATTTGTAGCTATACTTCAAAGCATTCCAGACAGATAACATGTCACTTGATGC
TTTGTAACAGTGTGCAGAGACTGAAAAAAATCAGTATTTTTTCTTTGCTGCTGTAAATTCTAGTTCCTTT
GGTAAATGGTGGTTTTATGTAGATTAAGTATGAAACTAAGAAAAAGGTTTAAGTAACCACTAGTCTATAC
AACTCACTAAAAAGTGATTAGTTCTGTGACTTACCACACAGGCCACAGAGCAGGCCACTGTAATCAGATG
GCACAGAGACTTCTGCATAGTGATTTCCATCATATCTCACCCACAGTCCAAAGTCAGTTTCCAAGAGAAC
ATAACTACCACTGTTTTGAATACTGATTTTCTTTTCAATAAAGATGGGTAGGTTCATTCTGCTGCCATTC
ACCTACAAACCAAGTTGTAAAAGTTAGTGTATAGCTGCTGAAACTAATCCAACTACAAGCAACTGGAATA
GTTTAGTGAAAGATGTCATTTTGTGCACAAAGAGAATGGGACACAATTAGATAATATTTCACCTTAGCAA
ATTTTTTTGAAATCTCATTAAAAGAGGTACATGTATCCAATGACAAGAGAAAAATTACTATAAATTCTAC
AACAAACAAACAAACAACAAAAAAAATTAATTATCTGCATGTTAGTTTAAAGATGTATTTCAAACAAGTA
TTTTCTTACATTCACTTTCTTGCTTTTCAGCATAGAAATCTGGTAGCCATAGACTTCCACTTGCACTGAC
TTCACATAAGAGACTTTGGTGTTGGATCCTCTGTGCTCATTTGTTGTGGACACATCAAAGTATGGAATAT
TCTGAGAGATGTTACACACTTTGGAGAGGGTGTAAGAACAGTTTCCCATGAAATTATGAACTCTGTGATC
AAAAGTGTTGTAATGTGGATCCCCAGAGGCACTGCAGGAAGCATGGCCTGGGAAGAAAGACAGAATAGTT
TCCTCTGAATCTCTGCTGTAAATGCTGCAGTCACTTTGTTTCACTTCCTAATTGCTGTTCAGTTGTGATA
GTGATGGCTTCAGTCAAGAACAGAGTGCAAAGGAGGTATGCAAACTTTTCACAGGAGAGAACACCCTTCT
ACCATAACTGGAAGTCACCCACATTCTGTGACAATTCATGAAGAGCAAGCCATCTGATCCAGAGACTCAT
TTTCTGTATAGTAGAGACACACCATATGAAACATGATACCTAGAAGTTGATCTGAATCATGAACATCCAA
ATAAATAGGATTTTCATGCCAATAACTACCTTGAGATTGAAAACAAGACATTCCAAAACCTGGGCTAAAT
TCCAGTCTTTGTACCCCAAAAGAGTATGTACTCTGGTGTACCAGAAATCTCTGCTAGAGGCCAATGAAGA
AATATGAGAAAAAGGATATTAAGACATCAGTATATAAACTGGAACAGTGAATTTGCAGTGGCCAATTCTG
AAAGGCTATCTCTGAATATATAGAAATAATCTACGCAAAAACATTACAAGACTGGCTCCTCTGTTTTGGC
AGCTGGAATGAACTGCTGCAATACCAGCTGTCTAGAGTTCTGTAGTTATATCAATTATAGAAATTAAGGA
AAACCTCTGACACTCCAGCATAACTCTGTTAATTCGGCTGTTAGACCTGAAGTTAGAAAACTGAACAAGT
AAAATAGGCAAAAAAGAAAAAGGAAATAGGAAACAAAAACACATGAGCTTAGCTGGGGTGTGGATGGCAT
TTGGGGAAGCTTAGAAGAGCTGTCAGGATATGGGCTTGTAGGCTTTGGGGTTGATGGAACTGCAATAAAT
CAGAGTTAAAGGAGGTCTGATTCATCATGAGACATTTGCTCCAAGTGCCTGACACAAATTGCATCAAAAT
TCTCTATAATGGCAAAAATTACCACAGATAAATAGAGATAGTGAAGTGAAAAACTCACTGGAACTTGGTG
TAGGTGATGGAGTTGGTAAGTGTGAAGGTGGTGTTGAGATGTCTGGTAAGGGTGTCCCAGGTTTTGGAGT
TGATGGACCTATTGTAGGAGAGGTATAGCAGAAATTAGAATCAATATGCAGTTTTACCCCCTGAAGATGG
TACACCCTTTCCAAACACCTGAGTGCCATGACAGTATTAACCCAGGACAAAATATACACAGTAACTATTC
TAAAAAAACTGTGCCAATCAAGTACTCCCCGAGCAGTATGGCTGGGGGAAAAGCAAAGTATAGACCCTTA
AAATGGCAATTCCAAACCCTAGCCAGGGACCTTGAAGGAATCGAACAAAAGAGATGCCAGAAAATGTACA
AAATGGGCTCAGGTCATGACAATACCTGAGCTTGGTGTGGTTGTTATTGGGTGTGGGGGCGGCGTTGAGA
TGTCTGGCAAGGGTGTCCCAGGTTTTGGTGTTGATGGTCCTATTGCAAGAGAGAGGTAGTGGAGCTTAGC
ATCAACATACTGTTTCATTCCCAGTTGCCAGTATGCCCTTCCCAAACACCCCAGTGCCATGACAGCAGAG
GTACTGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCTAATCAAGTACAATCCCAGCAGCGA
GGCTAGGGGAAAAGGAAACCACAACCCTGTTTCTACAGCCATGCCAACCCCTAGCCATGGACCCTGATGG
AAATCAACAAAAGAGTTGTCAGAAGCACTGCAAAACCGGTTCGCGTCATGACAATACCTGAGCTCGGTGT
GGTTGTTATTGGGTGTGGAGGCGGCGTTGAGATGTCTGGCAAAGGTGTCCCAGGCTTTGGTGTTGACGGT
CCTATCGTAAGCCAGAGTGAGAAGAGATAATATTCAATATGCTGTTTAATTCCAAGCAGCTGCAAATGAC
TTCAAAAATACCACAGTGCCATGACTGCATAGGCAAAGAACAGGGCATACACAGGGACTCTTGTAAGAAA
AACCTGTGCCACTCAAGTACACTCCCAGCAGAGAGGCTGGGGGAAAAGGAAACCACAACCCTGTTTCTAC
AGCCATGCCAACCCCTAGCCATGGATCCTGATGGAAACCAACCAAAGAGTTGTCAGAAGCACTGCAAAAC
AGGCTCGCGTCATGTCAGTACCTGAGCTCGGTGTGGTTGTTAGTGGGTGTGGAGGTGGTGTTGAGATGTC
TGGCAAGGGTGTCCCAGGCTTTGGTGTTGACGGTCCTATTGCAAGCCAGAGTGAGAAGAGATAATATTCA
ATATGCTGTTTAATTCCAAGCAGCTGCTAATGACTTCAAAAATACCACAGTGCCATGATCGCAGAGGTAC
TGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCTAATCAAGCACAATCCCAGCAGAGTGGCT
GGAGAAAAAGGAAACCACAACCCTGTTTTTACAGCCATGCCAACCCCTAGCCATGGATCATGATGGAAAT
CAACCAAAGAGTTGTCAGAAGCACTGCAAAACAGGCTCGTGTCTTGACAATACCTGAGCTCGGTGTGGTT
GTTGTTGGGTGTGGAGGTGGCGTTGAGATGTCTGGCAAGGGTGTCCCAGGCTTTGGTGTTGATGGTCCTA
TTGCAAGAGAGACGTAGTGGAGCTTAGAATCAACATACTGTTTCATTCCCAGCTGCCAGTACGCCCTTCC
CAAACATCCCAGTGCCATGACTGCATAGGCAAAGAACAGGGCATACACAGGGACTCTTGTAAGAAAAACC
TGTGCCACTCAAGTACACTCCCAGCAGCGAGGCTGGGGAAAAAGGAAACCACAACCCTGTTTCTACAGCC
ATACCAACCCCTAGCCATGGACCCTGATGGAAACCAACCAAAGAGTTGTCAGAAGCAGTGCAAAACAGGC
TCGCGTCATGACAATACCTGAGCTCGGTGTGGTTGTTAGTGGGTGTGGAGGTGGTGTCGAGACGTCTGGC
AAGGGTGTCCCAGGTTTTGGTGTTGATGGTCCTATTGCAAGAGAGAGGTAGTGGAGCTTAGCATCAACAT
ACTGTTTCATTCCCAGCTGCCAGTACACCCTTCCCAAATACCCCAGTGCCATGACTGCAGAGGTACTGAA
CAGGGCATAATCAGGGAGTCTTCAAAGAAAATTCTACTAATCTAGTACAATCGAAGCAGAGAGCCTGGGG
AAAAAGGAAACCACAACGCTGTTTCTACAGCCATGCCAACCCCTAGCCATGGACCCTGATGGAAATCAAC
AAAAGAGTTGTCAGAAGCACTGCAAAACAGGCTCGCGTCATGACAATACCTGAGCTCGGTGTGGTTGTTA
GTGGGTGTGGAGGCGGCGTTGAGATGTCTGGCAAGGGTGTCCCAGGCTTTGGTGTTGACGGTCCTATTGC
AAGCCAGAGTGAGAAGAGATAATATTCAATATGCTGTTTAATTCCAAGCAGCTGCAAATGACTTCAAAAA
TACCACAGTGCCATGACTGCAGAGGTACTGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCT
AATCAAGTACAATCCCAGCAGCGATGCTAGGGGAAAAGGAAACCACAACCCTGTTTCTACAGCAATGCCA
ACCCCTAGCCATGGACCCTGATGGAAACCAACCAAAGAGTTGTCAGAAGCACTGCAAAACAGGCTCGTGT
CTTGACAATACCTGAGCTCGGTGTGGTTGTTATTGGGTGTGGAGGTGGCGTTGAGATGTCTGGCAAGGGT
GTCCCAGGTTTTGGTGTTGATGGTCCTATTGCAAGAGAGAGGTAGTGGAGCTTAGCATCAACATACTGTT
TCATTCCCAGCTGCCAGTACACCCTTCCCAAATACCCCAGTGCCATGACTGCAGAGGTACTGAACAGGGC
ATACACAGGGAGTCTTCAAAGAAAATTCTACTAATCTAGTACAATCGAAGCAGAGAGCCTGGGGAAAAAG
GAAACCACAACGCTGTTTCTACAGCCATGCCAACCCCTAGCCATGGACCCTGATGGAAATCAACAAAAGA
GTTGTCAGAAGCACTGCAAAACAGGCTCGCGTCATGACAATACCTGAGCTCGGTGTGGTTGTTAGTGGGT
GTGGAGGCGGCGTTGAGATGTCTGGCAAGGGTGTCCCAGGCTTTGGTGTTGACGGTCCTATTGCAAGCCA
GAGTGAGAAGAGATAATATTCAATATGCTGTTTAATTCCAAGCAGCTGCTAATGACTTCTAATATACCAC
AGTGCCATGACTGCAGAGGTACTGAACAGGGCATACACAGGGAGTCTTCAAAGAAAACTCTGCTAATCAA
GTACAATCCCAGCAGCGATGCTAGGGGAAAAGGAAACCACAACCCTGTTTCTACAGCCATGCCAACCCCT
AGCCATGGACCCTGATGGAAACCAACCAAAGAGTTGTCAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGTCCGGT
ACTCACCGTTCACCTGGGGCAGACACAACGCAATGCACCGTGGACTCCCTGGGGCCGTCAGCCCCATGGG
GACTGGCTCCCTTCTCACACAGAAACTCCTGCTGCTTGCAGGTAAGACCCTGCCCAAAAAGACCAGCCTT
GCCTTGCAGACCCACCGCGCACACGCCAGACCCACTCCCTTACCAGCACTCGGCTCCTCTCCTGCTTGAG
TATGGTAATGCGCTCTCCATACACATCGACCACGACTTCACGCACGTAGGACACTCGGGGGTTGCCACGA
TGCTCGTTCTTTGCCTCAACGTTGAAGTACGGCACAGAGGTGTTGGAGCACACTTTGGCCAGGGTGTACG
TGCAGTTCCCCATGAAGTTGTGCGTCACCTTGTCAAAGGTGTTGTAGTGAGGGTCCCCGTGGACGTGACA
GATGCCGTAGGAGTGTTCCAGGCACTCGGGTTTCCCATTCTGCACACCACAGAACTGGCCAGCAGGGCAC
GATGCACTGGAGCACTGGACTTTGCCTCCTCGCGTGGGACATGTGCACTTGGACGAGCAGGTGTTGTCCG
TCCAGAACTCTGAGCCCACCGGGTAGTGCTGCCCGTTGTGCCAGCACCCACACTGCTGGATGGGCACACA
CTGACTGTTGTAGAGCAGGTACCCGCTGTCGCATACACATCCCTCCACGCACGGCAAGCTGCAGTTATAT
GGTGCCGTTGGGCTAACACACGTAGCAGGGCAAGCTGCAGCACAGGGCTCGTAGTGGCTGTTGGCTGGGC
ACGTGATGGCTGCCAAGACAAAAGGAAAAGCAGTGAAATCATCAGTCATCCTACACCCGCAGTGCTCCCA
AGGTAGGCCAGATTCACTGGGGGCTTTGGAAGGATGCAATCGCCCCACAGCTCCTCCCAGCTGCCCCAGT
GGGATGCCTTCTACATAGAAAGAACCACACCGGCAATGTGACCAACAAAGGGTGCTCCCACACTCACAGC
AGCTAACCAGCTCAGAAAATGGGGTGCTTTTCTGTTTCTGACTTACGGCAGAATGTTGCGTTCCTCCACA
CAGGGATCTGGACACCAAGCGCCTGGCAGGCATCTGCATAGGACTGCAAGCTGTTGCACAGCGCCTCGCG
ATCAAGCCCCAGCTCACACAGGTCATACAGGCACGTGTCGAAGTAACCGCTGGCACTCAGCACGGCATGG
CACACCTCAAACGGGCCGCTGGTGTCCGTGAGGAGCCCACAGAAGTTGCTGCTGGCAATGACTTGCTTGT
CCGTTTCAGTGCAGGCTGGCGGGGACTGCGTCCCAGCTGAGCAGCTATAGTGAGGCAGAGAGAACAGCCC
CCGGGTTATCTGTGGGCTTCTCCTGGCAGCTCCACTCCACCCCTCCCCAGACCTGCTGGGGCACTACACT
CACCTGCTGTCATTAGACACCTGCCAGCTGTTACCCAGGCTGGTGGAGTCTGGCTCCAGCACCCCGTCAG
GGTTCAGGAAGTCGTCTGCTGCCATCCCGTTGTAGTTGCCACACATGCCACAAACCTTCTGCCCAAAGGT
GCTTGGAAGCGTCACCTCCACCCGATGGTTCCCATCAAACTTCACTCTCAAGCCAAAGTCTGTTGTCACC
ATGGTGTAGAACCCGCTGGACGAGACTTGCACGCCCACAGATGGGGTGCAGGGCAGCACCTCTGGCTCGC
CGTTGACCTGGAAGGACAGAAACCCAGACAGGGTTGGAGCAATCCCTTTGGCACGGCTCTCCCCCGGCAC
AGCCCCGCCACTGCTCCCCTTTGTGCTTACCGTCACCACGCCACCCTTCCCAAGCCTTATGCGCTGGCCG
TACACATCAACGTCCACGTACTGGACGTAGGACACGCGAGTGTTCCCTCCTCGGTGTTCATTGGCAGCTT
CCACGTTGAAGAAGGGCAGTGAGCTGTTGCTCTCGCACAGCTTGGAGAGGGTGTAGGTGCAGTTGCCCAT
AAAGTTGTGGATTTGCTTGTCAAATGTGTGGTAGTGAGGGTCTCCCTCCACCACACACGTTCCTGACAAA
ACAAGCACCAGAGAAACCCTTCAGTTAGGCACCTCCTTCCAGCGCTTCTCCCCTTTCTACAGTGCTAAAA
GCACTGCGTCCCAACGACCGCTCTCCTCCATTCACCTGAGCCTGGCTGTGGTGTTGGTGTGGTTATTTCT
GCCACAGGTGTGGTGGTGCCGGCGCCTGGTGTTGGGGTCACATCGCCTACAAAAGACACAGTGGCACATG
CCAATCAACCCTCCTTGCCCACGCCCTGTTCAAGGCAGAAAATGCACTGACTGCCACTACAAGCAGGCTG
CCTTTCCCCAGCCAGTGTCACGTACCTGGACAGTATCCCTGTTCAATGGACAGATCATCCAGTGCTACGT
CACTGCGGAAATCTTCACCTATCATGCCCTCAAGGAGAATCTAAGAGAAGGGTTTTTTTCCAGCATTATT
TCCAGCCATATACACTGAGTGCTTTTTGAAAGCGCATGGCAGTTAGAGACCCTGTTCCTACCTGCAGGTT
TCCTGCACTGTGCACTGTGACCTCTCCCAAGATCCATCTGTCTCCTTTGCTTCCTGCTTCTTTCCATACC
AGTGTCGATATCTCGTTGTGTAGAACGTACACCCGCAGGGCCATGGTTTCAGCCACTCCGTACATGTGAT
ACCAGAAGCGGAAGCAGTGTGGTCCCGTTGAATTGCAAACTGTGCTGACCAAGATGGCTGTCTCACCTGG
CTTGTGCTCGTTTCCTTGCAAGTAGATATAATACCCATCTGGAAATAACACAGTGACAGTTTCTCATATT
GTTGAACCAGGAGAAGGCTAATTTACTTCATTTACATTTTTTGTGCTAGTACAGTTTTCTGGGAGATATA
ATGACAGTAGTCAGCAAAAAGTATTCATGCTACTGAGACTAGATCACTTATCAACAGAACAGGTCGTGTC
TAGACAGCTCTGGCCAGTACGAGTGGGGTGTGGGAGTATGAACAATTAGCCAGTCTGTAATACTTCAGTA
TACCTCCACCCAGCACTGCCACTAAATTTCTGCTTCCCAAATAGATTTCCAGCAGATTAAATTGTAGTTC
TGCTTTGGGAAAAACTAGCATCTTGAAGTACAGGCAGGCTCTGAAGTGATCCAGTGTCAGCCTGCATATG
GTTTACTGAAGCCTTTCTGCAGCTGAGTCTACAAACATATGTTACAAACATTACATGCCCATAAATGTGC
TGCACTGTTCATCCACCCCAAGACAAATTCCTGGCTGCTGCAGAAAGCTCTGGACAATGCTCAAGAAATA
AGTTGCCTGGATTTCCTGTTAGAAGAGCCTCTTTGTGCTCTTCCCAAAGATTGCTGTATCTGAAATAGAA
CCAGATAGCATTTTCTGTTCCTACCTCCTGTTGTGTGGTCATGGGGAGGGCCGGTATTTGGTGAGAGTGT
TGGTCCCTTGTTCCTTATCCAGTCAAAGTTGTCATTGATCGCCTGTTCCCACTCACAGAAGTCAACATCA
AATGTGCAGGACATATTGCAGGCTGTTGGTGCAGCTATATATGTTGCAGAAAGAAAAGCAGTTTCCACAA
TGAGGTCTGGAATCTTCTCTCTGTTCTGATAATATCCTTTCAGATCTAATGCATTCATGTCCATTAAACA
TTGGAAGATGTTATGGAATGAGTAGAATTAATCCATACAGAAGGATGGATAATAAAATATGAAATTCTAA
GTGTCTGAAAGTGTCTGTTATTTGGCAAGTCCGTTATTCAGTTGTTACCATAATGCTCTAATATCCTCAG
TTTTTACACTGTGCCACAGTCTTAATCTTTGGTCACTCAAAGCTCTCCTACTTGGAACAGTGGTATTCAG
CTGTTCAAAGTGTAGCTTGGGTAGAAATCTAAACACTGTAAATGGGAATGGTCTGACAGTTTTTTTATGG
TTTCTAACCTGCGATGAGAAATGCTGTCTGAGCTAGAAAGAGGTTATGGGATGCCTTTTGATATCATAGA
CTCATCCTGTTCCTTCTGCTGGTTTTGGCAGTCACATCTTGTAGTACAGAAGAAGCAGTTTCATGTCTCC
AACTTTACTACTGTGTTGACTTCTATGTCCACGCAGATAATAAGGGTCAAACAGTGTTATGTCTGGGAGG
CTAAATGGCCTTAACTTGCAGGATACCTTATTAATTTTAATACTCTATACAATTCTTAAGTTATTTCACA
TTTGGTGCAAATCTGGAGCTGTAGACCAGTTCTGTAAATGTTGTATTAACCCTTCTCAAAAATGTGAAAT
ATTTTAATGGAGCCAAAGTGGTGATTTGCTTGGAAATAACACAATAACTTGGTTTGGAAGAAACCAATAA
GTGCTTAGTTTTCCTTGGGTGATCATTTAACTGGCAGCATATGCCAGCTTTCATGATCCAATAATGAACA
TGTTAAAAAAGTTTAAACATTATGGCTTAAAGTGAACACTGAATGACCATTACAGGTGTAGATTAGAAGA
CTGATTTCCTCAGATGCTAAAAAACCAAAATATTTCTGTCTTCCTGAATTGCCCTTTCAGAATTTCTGTT
CAGCTCTTTAGTCATTTGAGTTTACATTTGTAACATGAGGATATAATTGGATGTCTATTTGCTGAATGAC
CTATAACTTACCGCAGATGGTATCTTTTCTCCATTCTCCCAGATTTACACCTTCAAGCTCACAGGCAGCA
GCATATGCCTCTAAAATTTTGCAGAATTGTTCAGAATTCCCCTCTGTGGCACACAGGTCATAGACACAGC
TCTCAAAGTACAGCTGTGGTGGAATGTACCAATGGCAAGCCTCAAAAGGTCCACTATTTTTCAAAATAAT
TTTACAGATTTCTTCGTATTCTTTCTCTTTTCCTGTGTCACAGGTGGGTGGTACAACTGCAACTGGATTA
CATCTGTATTAAAAATAAACAGCAGTATACTTATATTTTGCTCAACCCTGCAAGGTAAACCTCAAGAGTA
ATAGAACTCTATGCCAAATTCTGTGGACTTATTGTGTATTCTTGCCTTTAGTTTGTCAAACCCCAAAGTG
AGAAATATTGTAATTCGACCCACAGTTCCCCATTAAGAAAACAATAGCGAACTGTTGAGATGTAACCACC
TGTCCATAGACAGAAGTCCAAATGGAATCAACCATCCAGATATTTAGCTCAGATAATTTCAGTTCACTAT
TGCCCATAGTAAGATCACTACTTTGCATATGAAACCATTAATAGGCAAAGAAATGCTCTGTGTCCCACTT
AGTCAATGTAGAATTACATTAGAAATAGAAGCTGAAGAAAATATTTAGCTTTCTTACAGTTAGATGGTAT
AAACAAGGTATTTTAAAATAGAAAGATAAGAAGAAATGAAGTTAACATCCAAGAAAAGAGCTTGATTTCA
AATAAACTTTATGTAGTTCATAACAAAAATCTTTGAAGGGTAATTTTAAAATTTCAGTGCTTTCTACTGA
AATGTAGAATTAGTAAAAAGGTTAAGATTCTAGCCAACATAATATCAAAAAAGGGATTCTATGGAATGCA
TATCATGTATTTTAGTTAATCTCTTAAGCAGATCTATGACATATTTTGTTGTTTCCTATAATATTATCAT
ATACTTACACCCAGTTGTCATCCTTCAGTAGCCAGCTGTCACCAAATTTATTTGGATCCTGTTCTAGGAC
TTTATCCGGTTTTAGAAAATCATCCAGCTGGTTGTCATTGAATGTCCCACACAAACCACATAACTGTCCC
CTGAGACTTTCATCAACCTGAATAAAAAGGTCCTGGTCGCCATCGAACTTCACCTGTATCCCTACAGAGG
TATCAATGACTACATAACGTCCTTTTACAGCTACTCTGGCTCCATTCTTCAAATCCACAGGAGGGAAAAC
CTGAGCTCCATCCACCTAAAGAAAAGAAAACCAACACAAATGGGTAAGTCAACAACGTTCCTAGGAAAAA
AAGGAGAGATCTACACTGATAATACTGCAAGTTTTCTTCATTGAGTCAGCCTTATCCTAAGGAGCAATGT
AGTCACAGAAGTTTGAAGGGACATTCATACTTGTCCCATATCTTGAATTCAGGTTTGTAGAACAACTTGA
GCTCAGCACTGTTGCAACTACACGTATAGCAATACTTGGAATAGCTAGTTTAAAGCCAGCTCTGGTATGT
AAGAGAGCATATAGCAAAAATGTTATTTCTGTTCTAAAGACCCAAACTGTAAAATGCAGATGGGCATTTA
GTTCAGCAAAAGCTTTCTATTAAGGATAAAGCTCCAGATGGAGCTGCTTTTTCTAAAATGTTGTGCATAA
AATATTTGAAAGGATATAATGAAGCTATGCTGATATCTACATTTCTGAAAAAATATTTCAGTGACTTCTT
TCATATATATTCTTGGAGGTGAATAAAGCAGAGAAACCTGATTCTGTTATTTTGGATGGAAAAATGCAGT
GCACTGATATGACTGATTATTTGTGCTTAAAGTGGAACACTTACAAAGGTCTCTTTGTCTCTCCTCACAA
CAATATGAAGATTTTTCAGTGTGACAGCAACTGAATCCAGGGCTGTCCAGTTTGGGTTTCCTCTATGCCT
ATTTCTGGTTGTCACTGAAAACCATTCAGAAGAGTTTGTGCAAGTCTCGACAACTGTATAATTGCAGTCT
CCTTGGAAATGATACAGTCTCCCATCAAAGGTTACGTAATGTGGATCCCCGAAAATGTGGCAGGTGCTGG
GATTTAATGGATAACACCCCAGGAGTCCATTCTGCACCTTGCAAACTTCATTTGCTTCGCATGTGTCTGA
ATTGCAAAGCACAGTCCCATTTCCAACACATATACATTGGCCTGCACAGTTTGTTTGCCAAAAAGTTTCT
CCTTTCTGTAAGAGAAGACAAAATAAAAAGAAGTTAAAACAGTTCATGTTAATGATCAAATGATCAAATG
TAAAATAAAACCTCTGAAATATGTGATAGAACAAACCTCATAATATTTGTCATTCTGTAAGCAGCCACAG
TTGCTCATAGATACACATTGTGCCCCACTGATGACAAAACCATCGTTGCATTCACATCCCTCTACACAAG
GTTTGCTGCAGTTGTTTGATGCAAGAGGGTCAGTGCAGGTTGCAGGACATGCACTGGTGCATGGATTGTA
ATGGCTGTTAGCAGGACATGCAATAGCTGAAAAAGAATAAAAGGCTCTCAAATGATAATTACAACTCACA
TCAACCCAAAATATGAAACTGAGATTATTTTAAAATACTGGCTATATTTTATCAGAAATATTATCAGAAT
ATAAAATTTATTTCCTGGTTTGAAATCAGAAAGAAAAATAATATTCTACAAGAGAAGGGCATGAATATTA
ACATATATTGTTGAAATTTAGCAAATTCAAATCAAAGAGAAAAATAAGTCACTCTATAACAGGTCCAGTA
CTTAGTTGGTACATGAATTATTGAAGCTATTAAATGTAGACAGGATACTAACAAACATATTAAAAGCAAA
ATATGTGATAAAACACAGAGGTGATTACATACTATCATGGGCTTACTGCTAACTCTATGACATAGATTCT
TTCAATAATGAGCACAGTTCTCGAAAGAGATCTGGCAGTAAAAAGAGTGGAGTGACCGTTGTAGAAGATT
ATTTCTCAGCATTTCATGGGGGAATGTAAGCAGAATTTAAGATTTCTAAGCTATGTCTGTTAACGCCTGA
AAGCAGACTTTTGAAAATGGAAGGAACTATTCTAAGATTCAGTTCTACATAGGTACTCACGGCAGAAGGT
GGCATTCCTCCAGGGGAGCAGAGTCACACCAGCTGCCTGGCATGCGTCAGCATAGGCCTCCAGCGCACCA
CACAGCACCTCCTGCGCACCACTCATGGCACAAAGGTCGTAGGAGCAGGTCTCAAAGTAGCTCTGCGGGT
TGATCACGGCGTGGCAGCTCTCAAAAACGCCTGGTCTGGCTGTCAGCATGCCACAGAACTGCTCGGACTC
GTAGAGCTTCTCCTTGTCTGCAGGGCAGGGCTCTGTTGGTGGGGGCACGCCGCAGCTGGGGTCACTGTCA
CTGCTTGGGACCTGCCAGCTCTCCCCCAGCGCATTGGAGTCTGCGGCTTGCTGCCCGTTGGGCATCATGT
AATCGTCCTCTCTCCGGTTGTTGAAGTTCCCGCACATGCCACACGTCAGGTTGAAGTAGCTGCTGGGCAC
CTTCACCTCCACCGAGTGGTCAGCGTCGTAGGACACGTGGAGGCTGAAGTCCGTCTCCAGGACGACGTAG
CGGCCGCTACTGCTCACGGTGATGGCTCCTCCTGCTGCGCTCACCGGCAGGGTCCGGTACTCACCGTTCA
CCTAGGGCAGACACAACGCAATGCACCGTGGACTCCCTGGGGCCGTCAGCCCCATGGGGACTGGCTCCCT
TCTCACACAGAAACTCCTGCTGCTTGCAGGTAAGACCCTGCCCAAAAAGACCAGCCTTGCCTTGCAGACC
CACCGCGCACACGCCAGACCCACTCCCTTACCAGCACTCGGCTCCTCTCCTGCTTGAGTATGGTAATGCG
CTCTCCGTACACATCGACCACGACTTCACGCACGTAGGACACTCGGGGGTTGCCACGATGCTCGTTCTTT
GCCTCAACGTTGAAGTACGGCACGGAGGTGTTGGAGCACACTTTGGCCAGGGTGTACGTGCAGTTCCCCA
TGAAGTTGTGCGTCACCTTGTCAAAGGTGTTGTAGTGAGGGTCCCCGTGGACGTGACAGATGCCGTAGGA
GTGTTCCAGGCACTCGGGTTTCCCATTCTGCACACCACAGAACTGGCCAGCAGGGCACGATGCACTGGAG
CACTGGACTTTGCCTCCTCGCGTGGGACATGTGCACTTGGACGAGCAGGTGTTGTCCGTCCAGAACTCTG
AGCCCACCGGGTAGTGCTGCCCGTTGTGCCAGCACCCACACTGCTGGCTGGGCACACACTGACTGTTGTA
GAGCAGGTACCCGCTGTCGCATACACATCCCTCCACGCACGGCAAGCTGCAGTTGTATGATGCGGTTGGG
TTAACGCATGTAGCAGGGCAAGCTGCAGCACAGGGCTCATAGTGGCTGTTGGCTGGGCAGGCTAGTGCTG
TGTAATGTAAAAGATTAATACAGAAGTTTATATTTTAAATATTGAAGTATTTGGGTTTATTTAATGGCAT
ATTCTGTTTTGTTCTGCAGTCTTGAGGACACCCTGGAATCCTGTGGCAATGCAATCCTTGCCTTTTCTGC
GTAAGTGCCCTTTAACATAGTTCATGGTTGTGGCTCCTAACAATTTCTCTGTCTGAGAATTTCTTATGAG
ATCTTCTATTCTGGTCATTGTTTGCTACTTTTTCCAGCTGAGGATATCTGTGTCATGTTATTTGTCCTTG
TCATGTGCCCCATTCAGGCTACAGGTCTGATAATTCTAATCATATCATAGCTTCTGAATTCATACTTTTT
CAATCCCTGAAGTTCTTTAATGTTTGTTAAATAATCGTGATGTACATGCACCCTATTCTGTTTTTATACC
TTGCTACACATGTCACTAGTTAAAAAGAAAAACTAAGCTAATTCGAAGTTCTTCTAACCTCAATCTTCAG
TCTCTTTTCAGATTCTGTACACTTATGTGATGTAGTTCAGTTTTTAATGACATGCCCCACTCTATTTTAG
TCTGCTTCCTTAAGCAATAGAGATCATATAATTACATTGTATATTTCCCTGTCCATCCTCTCATTAATTA
GAACTGACCCAGAAATAAGTGAGGTTGGCAGAGGACAAAGGCATAGTTTGAAACAGGAGTAAGGCAAGCA
GGAGAGACCCGCAAGCTCCTGCAGCAGAAAGAACAGCTACAGAGAGGGTTTATTCCTGCTTAGAAATCAC
GGAATTTATGTGGGAGTAAGCCTTAATAATATCCTTAGCAAATAAAATGCTCCAGCAAGAGATTACAATT
AATTGTGAAACAAATATCCAGAGTAAATTTGGCTTCAGCCATTTCAGTGTTTGCAGACCAGCATCTTTCT
TGAGTCTCCTCATCTCCCTCAAGGCAGAGGGCACACAGGCCACAGCAGAATATACTGATTCCCAGATCTT
CTGCTCAGTTCATCAAGCATTTTTCTGCCTCATCCTGCTCTCTTTACATTGGTATAAATTCTGAATTTCA
GACATCTTTACTGTTCAGTCTCAGATCTATTCCTCCCACCAGTTTTGATTTTATCATTCCTGCAGGGGCA
ACAAACTGACCCCCTGACTGGCTACCTAGTCCATGTTCCTTCTACTTAGTTTAGCCAGTCTCAACATGTA
CTTCCACTTTTCCATAGCCCTGTAAGCATGTTCTCGTTTTCCTTTCTAGAAGCTACTTGCCTTCTCAAAA
TCCAGGGCCTCCCATTTACCCCATTCAGCATTTCTCTCCAGTTCTTAATCAAAGCTCCTTTCTCATTGCC
CCAGACCACCTGGATTCCTTATCTACAGCAGCTCTGCCCTTCATCTGCTCTGGCTCATATCCCTTCTGTG
TTCACATGAGATATCTGTCTTAATGAGCTTCTGCTGGGGTTGCTGCAAGAGAAGGCACAACCTCCTTTCT
GTTGACTGGGTGCCTGGTCACCCTACAAGGTGAAGAGGCTAAGAGAATAGAGTAAGGTATCTCAGCTTCT
CTACAGGGGGCGTGGGTGAGGCTCTCACCTCTACTAAGCATGTGCAAAATGAGATTTTTTTTTTCCCCTC
AAAGACCCTAAAGTTTATGCACAGCTTCCTCAGAAGGTAAAAAACCCTCAAAGTCAAATGTCCCTGATAT
ACTTAGTACCCCTGAACAAAAATATAGGGTAGGAGAAGAATTCAAAAGAAAAGCTCTCTACTTTCATCAG
TCTTTCTAAAGGACTCAAGGATTCAAGCTAAGGACTACACATGATTCTGAAATCTTTAGCCTCATAGGTC
AAGATTTACAGTGTTACAGGAATTTAAAAGAGTTTCTTGTAATGCTCTCTCTTCAGGCAACCCTAATGAT
ATCCTCCTGACAAATGGTAATTTCCAGTAATCTTTGATACGTACGGCAGAAGGTTGCATTTCTCCATGGT
GTCAGCTGGACTCCTGCAGCTTGGCACATGTCTGCATAAGCCTGCAGGTTTTGACAGAGGGCTTCGCTGC
TCAAATGAAGCTCACAGAGATCATACTGGCAGTCTTCAAAATAGCTCTGTGGATCAACTACTGAGTGACA
ATTCTGGAATGGACCACTGGGATCAGTGATCAGACCACAGTATTTATTGCTTTGGATCATATGTTTTTCA
TCAGCAGTGCAATTTGGGATATGGCCATCATCTGGCAAGCACCTAGTGTGGAAAAAATAGATGTGGAAAA
TGTCACTAGTGAACTTTGCATACAATTGCACTCAAGATGCTTTTCAAATCCCAACTAATAAAACTACAGG
GCTGGCCCAGACTAAGGATGACTTTTAAAATATTTGTATTTCAAATGGGATGGGAATTCAGATTTTAAAA
TGATCTCAACATTATAACTTTTGTAATGCCTGTAAGAAAGTTAATCATTAATTTTGGTTTTAGGTCTAAA
AACAAGCTTTGTTCAAGTAAGACAACTAGGAAAGGCAAACAGAAAGTAAAAGCATTCAAAGTTAAACATC
TATCATCTTGAAATGACACACTGTATAATTATAAAATAAACAGTGAAGTATCTCAGAAGAGTTTTAGCAG
GATCATTAATATGCTAAAATAGATGGAGTCCTAGTGGGCCATTACAGGTGTATTCAATTTTTCTCTTTTT
AAGTACACTTGAATTTGCTACAAGTTTTTTTCTTCCCAGATACGTGAGAGAGCTATCCAGGCAAGAATAT
ATTTTAATCCATATTCCTGACATTTTGGGCCTACATACAATAGCTGCTTAAAATACTGCTCGTTACATAA
CTCCCAGATAAGGCCAAACATCCCAATTGGAGTCCTACCTTGAGTCATTGTAAACTTGCCAACTGTTTCC
AAGGCTGGCTGAGTCTGTTTCCATCCCTCCATCTGGGTTAAGAAAGTCATCTTTTTTGTCCCCATTATAA
TTTCCACATATCCCACAGACTTTTGATATATAGGCACTGGGAAGAGTGATTTCTGCTCTGTGATCTCCAT
CAAACTTGACCTTCAGCCCAAAGTCAGTAGTAACCACAACATATTGACCACTGAGGGAAATGCTGACACT
ATTGCCCAAGGTCAGAGGGAGTACCTGGCTGACTCCATCAACCTACAGGAACAGAGAAGGACAATCAGTA
TAATCACTTTAAAATACGTATTTTTAATAGTGATTTTCTTAGACCATGTTCCTATACAGAGCATAGGGTT
TGTACAGTGAACCTGTACTAGCATTAAGAACACACAGTGTTGTGTTTCTGGGTCTTTTTGGGCCTTACAT
AACATGAATGCTATATGTGGACAAATGACCTTGGGCCTCAAGACCGTAGTAATGCATGTACTTATCTTCC
AGGTCTTAGCTGTGGTCAAAGTAAGCTTGGTGAACTAGACCCAGCTGGAATAAGTGCAAAAATTATCAAG
GTCTTTCAGCAGTAACTGCTTACAGGCTTTCGGGATCAAACAAGAGCATGGTATAAGATTTAAAAGATGT
AGCTAGAATGGAGTCTCAGAAAATTTTCAGGGTTGGACATATTTTGAAAACATTTTTTGTTCCTTTTTTT
TTCTTTTTTTTTTCCCTCTTCTTTTTCTTTCTTGTTAATTTTCACATGTGAAATGCCAACAGTAAGAAGG
AAGTGTTCAAATAAAATTTGCAATTTATTTTAAATTCAATAAAACTAACATTCTTTGAATATTTGTTCTT
ATTACATAATTAATATATATACATTTCAACTTAGAAACATATATAATGAAAACAGTAAATACAGTGAAGA
GATTGAAAAGAGAAACTTTTGGACAAATGCAGATATCAAGTTCACTATGCATCTAAGTTGTGAAGTCTCT
GTATATTTAAATCCTTATCTCCTGTCCTATTTTCTTTATAAGAAGAGTGATAATGAAGTAAGGTGATCTA
TTCAATTACTGTGGGTTTTCAATCAAATGTGTATCTGCTACCTTGGCTCTTCATGGAAATAAGTTATAAG
GAAGCCTTAAAATACAGCAGCTGGTCCAGTGACACAATCAGAGTGATGTGAGTCATCAGGCAGCCTTTCT
GGGACTCTCATCTGGCTGTGCTTTATGGCAAGTGCCAGGTATTAAGTGAAATTAGCAGTGATGAGGAACA
ACTAGAAGTCAGAGATGAGCATGCAGCTTTACAGCCTGGGAAATATCTACCAGCACAAGCTCTGTTAATG
AAAGGCCTTTTTTATTCCCTGCTGCAGACCAAGTTTAGGAATGGAGGTCAATCTCTGAATGGAGGGCTCG
CTCACCAACTCCACCCAGAGTATCAGCTCTACTCTTAACTGTTAGGAACACAGAAATACAACGTTTTGTA
TTTTCTTTCTGTTCTGAAATCTCAATGCTTACTTTAAGGAACCCATAGTACTACTGTTCCCAAATATTTG
ACTGCCTTCTAAATATTGCTTTATGCTTGCCAAGAGATTTGTAGCCATTGTACTACAATTTCTTCAAAGC
ATGATTTACTGACAGTAGATAGTGAGATCCAGCCTTACCTTAACAACTCTGTTTTTACCCAGATTGATCC
TGTAGCCATAAACATCAATGTCCACAGATTGGACATAGGACACACGTTTGTTGCCTCTTCTGTGCTCGTT
AGCAGCTTCCACGTTGAAGTAGGGCAGTGAGCTGTTGTTCTCACACAGCTTGGAGAGCGTGTAGGTGCAA
GTGCCCATGAAGTCATGACGCTGCTTATCAAAAGTTAAATAATGAGGATCTCCATGTATAGTGCAAGTTG
CTTCTGGAGAAAGGAAAACAGAGTATGTTTCATTAAGATATTGGTGCATCCAGAAAAGCCAGCTCATACC
TTCGTTGTGCTGTGGGAAAACATAGTCATCCCCATTTTATAGGTTACACATGAAAGAACTGAAACAGCAA
AGACAGATTTTTATGGCCCAAGGCAATGCCACAGAAACACACTCAGATAAGTTAACTCAAGTAACACTGG
TAGAATGCACAGCTGCCCTTCTCTAAAGCACTGACTATCACATGTGATTTGTTAAAATTACCTGTCACAG
CAGGTGGTATAGGTGCCGTTGGTGTAGGTGTTGGTGGTACAGGGGTGCTGACTGCATCTGTCAGTAAAGA
CAAGGTAACAATAATTAGCATTATATAACATTACAAACTGAACACTACATACAAACCAACATACGTAACT
TTCAACTTACTCTTCACCAGTTTAAAGTTAGAATATCTTCACTGAAGAAAATGGAAGTCTACATTTAAAA
ATGAGGACTTTAGGTTCTTTTGAATTCTTAGGGAAGCTCATCTGACTACTAGCTTGGTGACAGTGACTGA
GCTTGTCCTTGCATGATTCTCTGAAACTGGAGATGTATTTACAATTGCATCTGTGATTCTCTCTGGTTTG
TATGTTAAAGCGTGAAGGTCATGTTCTCTGCAGGCCTTGGCAGGTCTGGAAATGAGTAGCCTGCCAGCAG
ATGCCATGTAACAGAAACAAGTGACTCTCAGGACACTGGTAGTTACTAATCAGTTTAATTTTTTCTACAA
AATGGCATTTATTTCTTAGGAATGTAATGAATGTAGGAGCTTTCTGCTGACAGATATTATAAACAGTGAT
GTTGAGTCTTTGCTGATGTCCATTACTGCTTGTTAATACATATTAATACATATTAGCATCTTCTCCACCT
ATTCACCTATCACAAAGACATATTCTAAAACAAGTGGTCCTTGTGGTGTATTAGTAAGTGTGTGCTAACA
ACCCCAAGGTGTACTGTAGGCATAGAGCTTAGCAGAAGGAGGAGCACTTCTCTAGTCTCTTGGGATTACA
TTAGTGCACCATCCGGTGGCTGAATTGTGTGGTAATAAGCCTGGAAAAATGAACAACTGGCTCTCCAAAT
GGGCCCCAAGTATTTTTCACAATTTTAAAGAAAATTACATCTTCTGAGGTTATAAACATGAATGGAACTC
TTCATTATCCAAAAATCCTAGCTAGAATAAATTTAAAACATTTCATTGACATTATATGAGTAGTGTTTTG
CAGAGATGTCTATTAAGACTTGATGATGTTCACAGCATGTTTCTTGTAGAATTCACATCCTGTGAGTTTT
GCATTCATAACAGTAAAGATGGGATTAAACTGTAATTCATTTCTGGCTTCAAGTCTTCCACAGACTGTGC
AACTCTAGACTGATTTATTAGAGGGTCAGAGATCAAACTCGAATTAGTGACGTATAATTAGGTATCACAG
TGCAAGTCAAAATTATTGATTCAACAATATACTTCTCTTTAATTACGCATGACAGACCTGTGTATTTTCT
GGCTCATACTGGACAGAGATTAGCTGTAAAAGATATTTAGAACACTGAAACCAGGAGTTGGACTCTATGA
TCCTTGTGTGTCCCTTCCATCTCAGGTTATCTTATGATCCAAGACCTTGGCTGGGTTTAGAGATGCACTT
CACACTTAAAGTTTGTTGGACCATGTGAAAATGGTCACATTTACAGAGAAGTGAATGGCTTAGTCTCTGT
GCTTGAGGCATTGAGGAAAGAACTGGGGAGAATTTACAATAAAAGAGGTAACATTTCTATGAAGTGAGCT
GGAAAGCCTTTTCCATTTCTCTTCTGCGGCTTGGAGTTATGAAGAGTTTCTATAACATAAATGGAATTGA
ATAATTGTCAACAGCTTAAAGAGAGATGAGGAAGGCACAAATATACACTTACCACAGGATCCATCTCTTA
CTCTCACATTGTCCAGTGCCGTATCTGCATACTCTGTCAGTCCTCGGACAGCCTCAAAAATGACCTAGGC
AGGATGCAAAGTCAATCGCTACATCAATTGTTCAGGCATATGTGAGAGAATTTCCCTCAACATGTTTTCA
GAATACTCTTTCTGGTCTATTTCTAAGGGCATCCAGGGAACCAATGGTGGAACACTTTCCCCAGTATTCT
TCAAGAAAAACTTGTAGAAGTTTTTTGTAGAAAAAGTAAGGCACCTAATGTCTTTGACATCATGATAAAA
TGAGGGAAGGAGGGAATGTGGGAAGGAAGGAAAGAGAGAGCAAATCAAATATATTCTATCTCCTAGCTAA
AAACACCAGTAAACCTCAGGGAGTTGTTTGTTTTTTCTCTTAGTTTATTTGCACAAAGCATGAGGGCATT
ATAATTACAAAATAGAAATCAAGAAAATTTTGGTTATTGTTTCTAGCTTATACATACAACCTTTTACAGC
CTGCAAAGGGAGAGCAAATCATAAATGCTGATGCTGTACAAACAAATTTATTATGAAAATATATTACCTT
TATCTTTCTCTGTGTTGGAAAAGTATGTGTGACAGCACCATAAATCCAAGAGGAACTTTGGTTTCCCTTC
CGACTCCATGCCGTTAACTCCCCTGTGCTGTCTTGGATAAGCACTCTCAGTTCATTCCTGTCTTCTGACC
CAAACATATAGTACCAGAAGTCTATGCATATCTTTTCAGAAACCACAATATCTGGGCTCTCAAGCCTGTT
AGTGTCGAATGGGATCAGATTGCTTGCTTCTTGGTAGATGAAATAGCCTTCTGCAGAGTGAAAAAAATAA
AAAAAATAAGTCTGAATCTTTCTAACTTGTTAGGTGTCACTTAAAACTGAGGTACATCTCAACTCTGGAA
GAAATCAAGCACATCATTAGCAGTACAAAGAAGCAAAGTAGCAAATGATTGTAATTTATACTGCAATGGT
TAGCACTGTGTAGTTAGCTAAAGTACAATGAGACCTGGAGAGCAAAGCAAATATATCCTATCTCATATGA
ACAGTTATTTTGGGATTGCAAATAAATATCTAGGCTTCTTTCAAAAGACAGCATTTCCAGACACAGTGCC
CAACTTATCCTTGTTCAATTTCCACTTTCCTCTGAGGAAAATAATGTGATCTACTATGCATCTGCTATAC
ACCTTCTCTTGCAAGATACGGTCTAATGATAAACAAATTTTTGGCATAAGGAAAATTCCAACCCCTCAAT
ATTTGGTTTTGTCTCAAAGCAGAGGGGTAAGAAAAAAAACACACAGATATTTCCATTAAAATGTTAGGTT
TGACATTATCAAAAGGAATCAATCTTTTCAAAAATCAGAATCTCACAATGTTTGATTTTGGGATTTAAAT
ACTTTACTTTGAACTTGTTAATTGTGAGATCACATTTTCCTTTACTAAGGCTGGAGCTTTTTGCTTCCTA
TCACTTCTCTCTGCTAAGATGAAAACTGTATTTTGCCAGTGCAGTTATGTCAAGTCTCTCCCATATGACT
ATGGAAAGGAGTAAAAAAGAAATGACCATATCTACATTATAAAGCATTACTCCACACACAGTATCTAATA
TTCAGTCTACTGTTTTAGGGCTCTTTTAATAATAAGAAAATGTAAGTAGCACTTGAAGTTATATGAACCA
TGCCAATTGAAAAAAATATATACTTATTGTAAGTGACCTTTCCAGTCTAGGGATAATTACTGAAAACAAT
ATGGCTACAGATTGATCTCCTATAAATGAATCTTCTAGTGCAAGGTTGAAGGTTCTACCATTCCATCTAG
TGATGGAGCCATATTAAACACTCTTTTAATGATAGGACCACATTAGACCTCAGTTTTTCAATCCCATGTA
GCTGAGGACGAGTTTTAAATGTATTCTTTGATTTCTGACTGTAATTTCCAATCTTGTAATGCTTTGCTAG
CATGAACATTATCCAGTGTCAGCACAGTGATCATTACATTTCTTGGCGTCAGCTGATATCACTGTAATTT
GATTATAAAACTTCTCCTGTGTAATGATCAATTGAATGTCACTGCATTCTCTGTGAGGTCTTAAGCAAGA
TGTAGTTAGCATGCTTCTACTTGCTTTTACTGCACTGATAAATATTTCCAACCTATGATGGGGAAAGCCA
GTTTAATCTTGTGACTGAACACGGTGAAAAGTCAAAGACAGGTGTCAACCAAACCGACACATTTCAAAGG
ATTTCATTAATCTCTTCATGAAAAAGAAAGAAGAAATGTAGACAAGCTGTATTCAGTAAACAATGAAGTA
AACAATGCCATCAACAAGCACTTACTTCCATCAGGATAGTCTCCATCAGGACCTGTTCCATCTGTTGGAG
TCTCATGCTTGGTTCGTATCCATACGCCCTGGTTCACAGTGCAAGGTTGGGTCCAGTCACAAAATGGTCT
TGAGTTGTTGTTAAAATCACATCGAGTGAGGTAAGCTGAAATGCAGAATAAGGTAAGTAAGGAAAAGGAG
ACACTATATTCAAAACCTGCCTTGGACATTAACAGCACTAAGTATAAACAGTTACCTGTTGCAGGATCCC
ATGATCTCAAGCCACCGGTTGGTCTGTGTTTGATCCTGCCAAGAAAAAGAACACAGTGATATCCCTACAG
ATTTAGTGACACAAAAACTGTTTAAGAGTGTAGAGACTAACAGAAGTCATCAGGTGTTCTTAATGAATCA
CTGCCCAGACTCAGGGTCTCATCTGTGTCTGACTCCTTCCCTTCCCATCTGTCCTTACTCCTTCATTAAG
AAGTTCTGAGAGCTAGAGAAAAAGTGTGATATGTTCACCTCATCTGAGTATAGAGGGATCCTCTCCCTGT
ATCTCTCTAAAGTGAAGGGAGAAAGTTCTACTAGGCACAGGGAGCAACAAGAGAGATGGCAAGGTACAGC
AAAGTTCGGATAATACCAGAATAACACAAAAGACTTGCTATTAACTTGTTTGTAGCACTGGCATCCTCAC
TACTGCTAAGCAAGATACGTAAACGCCAGTAAACTGGTGTGTCACAAAACTGTTATTTGTCAGAAGGCTT
ATGTTTCCTTTAACCACAACAAATATCTCATGACCACAAGCTGCCATCTCATTTAGTTTCTCCTGTAAGT
TATCCTAAAAATGCTACAGGGCAAGGGCAGTTAGAAATAGATATAGTCCCTTTGGTGCTAGAAAATCCTG
CAGAGGTGAGATGACATAAAACCAAAGCTCTGTCGCAGATCATAAGATGGCTCTATTCATTGGGTGTTTT
GCATCAGAAAGGGATTTCCAGGACTGCTAGACTGCAGTAGCTGCTCTTGACATGTTCTGATGGTGGTCTA
CAGCTTTAGGAGGCAGGGTCCAGTTCCTATCCCTGTGTTGCCCACCATCCCTACTGTCCTTGTTGTACCT
GACACAGTATAGAGAGAATCTGTCCTTAAGCAAAGCCATCACAGCAGATGTCCCCACAGCCCATGAAAAT
CTAAGCAGAGAGATTCAGAAAAACTAGGGGGATTCATAGTATCATATACAAACAGGATCTACTTCATCAC
TGAAACACACCAAACAACTGGAAAACAGACGAAGCTAAAGCTAAGGAGGAGAGAGGTGTGAAGATAGGTG
AAGTGAGAACATTTTCTTCTTTCCCCACAAGACAGCTGTAACAAGATGCCATTTAATCTCTATTCTATGC
TATCTTTAAACCGCTATATATCAAAGTCCTAAGGCAAAGGTTATTGTAGATATACTTAGAAAGTCACAAA
CATTTGATAACTGCAGCAGATCTCCCCTTTGGTTCAGCCATTAGAAATGTACAGTTTACACATTCTCTCA
CAGACAGCAACCACTGCAAAACTTGGCAATAACACAATTCAATTATTCCCTCCTTTTAAAAATAACATGG
TTTAATCGTACAGCATTCATGTGTAAAAAGATGAGAAAGGACATTTATAATTAACAATAACTACGTTTCC
TGAACTAAGGTAGATTCAGCATTGTGCTACTTACCCTGTGGTATTCAGCACCAAAATACAGAATAAAATC
AAAATGCATTGTAAGTGCCAGAGGGTCCCCATGCTAGAAGACAGTCAAGTCTTCTGTGTGGTACAGTCTA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Gallinarum relA/spoT mutant vaccine
7. IgG2b
  • Gene Name : IgG2b
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16016
  • Genbank Accession : AC160982
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 56717
  • Gene Ending Position : 60336
  • Gene Strand (Orientation) : -
  • Protein Name : immunoglobulin heavy constant gamma 2B
  • Protein Note : Also known as IgG2b; Igh-3; gamma2b
  • DNA Sequence : Show Sequence 
    >gi|121699722:56717-60336 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
CCTAGGCTCCCTGCCCAATCATGTTTCTGTAGTCAGGGGAGATCTTCTGCTTCAGCTCCACCACTGAGGA
GAAGATCCACTTTACCTGTGGGGAAGTGAGAGTCAGAGACCACACCCATGCTCAGACCCTCCAATCCTGT
GGGGAAGACAGAGGCCTGTCTCTTCTTCAATTCCTTTCATACAGGTAGGAGACTGGGTATGGGAAGTGGT
CAAAGTGGAGATATTGTGGGAGTTGAGGTGGAGATGGTATGAGATTAGCTCACAGAGTACCTTGAGTTAT
GGTGAGGCGAACATCAAGGGGATTGTGTGTTAGACAGCATGAACCTCATTAAGGCTAGTGTGGAATGCAA
GGAGGTTTGGTCTCATAAGACAGTATGAGGATCACGCAGGACAGCAGTAGAGACAGAAGGGCAGACTGTA
TGGGACAAAATGTGGAGTCACATAGAGCAATGTGGGATGGCTCAGGGAATGGAGGAAACGGTGTGTGGTA
CAGAGTGGGGACAGCAAAGTAACATCCTACTACATCAGGGGAGGAGTGAGAGGACATTTTGTGGTCAGTG
TAGCTATCACAGCAGAGGGTGGGAGACAGTGCCAACCTTGAAGAGTGTGACAGAGGCGCTGTAGCACACG
CTGAGCAGGAAGAGGCTGATGAAGATGGTGATGGTCGTCCAGAGCCCGTCCAGCTCCCCATCCTTGGCCT
CAGCACAGATATCATCCAGGTCTAGCCCTGAACAGAGAGGACAGTCAGTGTTTGTCCCAACATGGTTGAT
GCTGGGAGAGATTCCCTGTGAGGCAGACATGTCTGCATGAAGTCAGAGGAGAGATCTCCAGCCTGACTGG
GACAGCAGACCCTCCCCGGGGCCTGCTTTCCTTCCTTTCCCTCTGGGACATCAGTCTCAGCTTCCTCCAC
CTGAGTCCAATAATGCCTTGGATGGACCTCAGAGTGACCTGGATTATTCAAGTGCCCTCTATGCTTGTCT
CATCTTTCATTCCTTCAGTACAGGAGAAGGTGGAGGGTCAGCAGGGCCTGTGACCCAGGTCAACAGTGGC
TATACAGCTGGACTGACCCTTTGCTATTCTTCAGTCATCAGCAGAGCCTGTGGCTTTTCTTGCCTGAACT
TGGGAACATCCCAGTGTGAAGTGATTCAGACCATTATGGCTATGGTTTGAATTATTCATGTGGGGTTGAA
GCATGACCCCGAGGTACCAGAGATGAATCTGGCTTCAGAGGTCTTGTTTTGAGCGGAGAATGGAGTGTCC
TCAGAAGAGGAAAGGTGTGTCTGATTGTGATGGCAGAAACGTGGGCATTGAATTTTTGGCCGTGTGTATG
GTGATACATTGCTGGATGTAGGTGCTGTGCTGGGCACAGAGGAGTTGTCCCAGTGTGTTAAATGCGTTTT
GTGCTCACGAGCATGAGGATACATGTGCTATATGGACTGGATGGTGTTCAGCAGGGTCAGGATTTAGCAT
GAGTGTGGCTATCTAATAGTCCCTCCCTTGGGGCATCAAGAGCCATGTGCCTCAGCCCCAGGTGGCACAG
GCAGGAGTGAGCCATTTGTATGGGTGTGCAGACCTCGTCTGTGAAAAGCTGCCTGAGTTGAGAGTATAGA
TGACAAAGGTCTATGGAATGCCCCTGGCCATGTGTCACTCCACAATAGACTCAGAAAAACCCCTAAGTAG
AGAAATGGGGCAGGGGGACAGGAACAGCCTAAGGGAGGTGGGTTTGTGTGCTTATCCATTTGAGGCTTCC
TAGGATTATCTCTATGTCTGGCAGCTGCTGGTTCATGGGAAGGCAAGGAAAAGGCACTGCCTAAAGCCAA
GCCCCAACAGTCCAAGGCAGAATGACTCATGACCTCTGAGTCATGCAGTGCAGAAACCTCATTCTCTTCC
TATAGACAAGGCTGTCCTCCAACCCTGGCCAGACCTATCAGCCCGTGAGCTATGCTCTATACCTTGGAAA
GAACCAGGACAGTTTTACATGGTACCAGGCTTTGCTGGATGCTTTATTTATACAAGGGATGCATGGATAT
GGGTGCCAGTGTCTCTTAGGACCTGAGAGCTTTGTGGGTGCTGAGCTCATTTACCCGGAGACCGGGAGAT
GGTCTTCTTCAGGTAGTAATTTTTCAGACCCTCGTGTCTCACGTTGCATGAGAAGGAATCTGTTTTCTCC
CACTTGCTTGTTTTTATATCGAGCTTGCTGTATATGAAGTAAGAACCGTCAGAGTCCAGGACTGGTGCGG
TGTCCTTGTAGTTCTCCTCTGTATGCCCATTGCTGGTCCACTCCACACTGATGTCTCCAGGGTTGAAGCC
CACGACCAGGCAAGTGAGACTGACATCTTTCCTGGACAACTGCTCTGCTGGTGGCGGCAAGATGTATACT
TGTGGAGCTCTGACTAGCCCTGTGGGGTTAGAAACATACATAGAGGTCATGGCTGAAGTGGAAGGCTGTC
CACATAGACATTTATTCTTATGCCCATCCCAGCCCCCATGCAGTTGTCCTGCAGGTCCCACCTTTAATTT
TTGAGATGGTTCTCTCGATGGGTGATGGGAGGTCTTTGTTGTTGACCTTGCATTTGAACTCCTTGCCACT
CATCCAGTCCTGGTGCTGGATGGGGAGGGCACTGACCACCCGGATAGTACTGTTGTAATCCTCTCTATGG
GTTTGTGTCTGAGCTGTGTGTACTTCCACGTTGTTCACAAACCAGCTGATCCGGACGTCTGGGTCATCCT
CGCTCACATCCACCACCACACACGTGACCTTGGGTGTCAGGGAGATCATGAGTACATCCTTGATATTTGG
AGGGAAGATGAAGACGGATGGTCCACCCTCGAGGTTAGGAGCTGATGAGAAGAGATGGAAATGGATTTGT
ACATGGCTTATCCTCCATTACAGGGATTTTTACAGCACTTACCATTCTCCTGGGAGTGGAGCTCTGGTAG
TGACTTACCTGGGCATTTGTGACACTCCTTGCATGGAGGACAGGGGTTGATTGTTGAAATGGGCCCGCTG
GGCTCTGCAGAGAGAAGGCTGGATGTTACTGGTTTTTGAAGGAGAGAAGCACCAAACCAGAGAGAGGAAA
GTTTGTCCCTGGTATGGGCTTAGTCCAGGATGATCCTGGAAATGACAAGCACACCTATCCCAGAGGATCA
TGTCCTTGAGTGTAGGTGGTCATAGCTAGATGTTCCCAGGAGGGAGGCAGAGACAGTATCTCCCTCTTCC
CTGGGCTAAGGTCACCTCCTTGGTGATGGATCCCAGCCTGGCTGGTTTAGATAGGCTAATACCTTTGCCT
CAACTCTGGTGAATCCCTCCTCCCCTGAATGTCCTCTCACCAAGTTTTTTGTCCACCGTGGTGCTGCTGG
CTGGGTGAGCAACGCTGCAGGTGACGGTCTGACTTGGCCAGGTGCTGGAGGGGACAGTCACTGAGCTGCT
CATAGTGTAGAGTCCAGACTGCAGGAGAGCTGGGAAGGTGTGCACACTGCTGGACAGGGATCCAGAGTTC
CAAGTCACAGTCACTGACTCAGGGAAGTAGCCCTTGACCAGGCATCCCAGAGTCACAGAGGAACCAGTTG
TATCTCCACACCCAGGGGCCAGTGGATAGACTGATGGGGGTGTTGTTTTG
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhi phoP mutant vaccine
8. Ighg1
9. Ighv1-9
  • Gene Name : Ighv1-9
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 668478
  • Genbank Accession : AC073561
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 114583568
  • Gene Ending Position : 114583861
  • Protein Name : immunoglobulin heavy variable V1-9
  • Protein Note : Also known as Igg2a; Gm16697
  • DNA Sequence : Show Sequence 
    >gi|372099098:114583568-114583861 Mus musculus strain C57BL/6J chromosome 12, GRCm38 C57BL/6J
GTCTTGCACAGTAATAGATGGCAGAGTCCTCAGTTGTCAGGCTGCTGAGTTGCATGTAGGCTGTGTTGGA
GGATGTATCTGCAGTGAATGTGGCCTTGCCCTTGAACTTCTCATTGTAGTTAGTACTACCACTTCCAGGT
AAAATCTCTCCAATCCACTCAAGGCCATGTCCAGGCCTCTGCTTTACCCACTCTATCCAGTAGCCAGTGA
ATGTGTAGCCAGTAGCCTTGCAGGAAAGCTTCACTGAGGCCCCAGGCTTCATCAGCTCAGCTCCAGACTG
CTGCAGCTGAACCT
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhi phoP mutant vaccine , Salmonella Typhimurium asd mutant vaccine , Salmonella Typhimurium asd/rfc mutant vaccine
10. IgM
  • Gene Name : IgM
  • Sequence Strain (Species/Organism) : Sus scrofa
  • NCBI Protein GI : 164513
  • Other Database IDs : CDD:209398
    CDD:143166
  • Taxonomy ID : 9823
  • Gene Strand (Orientation) : ?
  • Protein Name : immunoglobulin mu chain
  • Protein Length : 403
  • Protein Note : Immunoglobulin domain; cl11960
  • Protein Sequence : Show Sequence 
    >gi|164513|gb|AAA51297.1| immunoglobulin mu chain, partial [Sus scrofa]
QNIQDFPSVLRGGKYLASSRVLLPSVSIPQDPEAFLVCEVQHPSGTKSVSISGPVVEEQPPVLNIFVPTR
ESFSSTPQRTSKLICQASDFSPKQISMAWFRDGKRVVSGVSTAPVETLQSSPVTYRLHSMLTVTESEWLS
QSVFTCQVEHKGLNYEKNASSLCTSNPNSPITVFAIAPSFAGIFLTKSAKLSCLVTGLVTRESLNISWTR
QDGEVLKTSIVFSEIYANRAFGQGEASVCVEDWESGDRFTCTVTHTDLPSPLKQSVSKPRGIARHMPSVY
VLPPAPEELSLQEWASVTCLVKGFSPADVFVQWLQKGEPVSADKYVTSAPVPEPEPKAPASYFVQSVLTV
SAKDWSDGETYTCVVGHEALPHTVTERTVDKSTGKPTLYNVSLVLSDTASTCY
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
11. IgM
  • Gene Name : IgM
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16019
  • Genbank Accession : AC073553
  • Taxonomy ID : 10090
  • Chromosome No : 12
  • Gene Starting Position : 171229
  • Gene Ending Position : 175133
  • Gene Strand (Orientation) : -
  • Protein Name : immunoglobulin heavy constant mu
  • Protein Note : Also known as Igm; muH; Igh6; Igh-6; Igh-M; AI326478; MGC107036; MGC107507
  • DNA Sequence : Show Sequence 
    >gi|121699722:171229-175133 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
GTCATTTCACCTGCAGGTGACAGAGACAGTGTGAATTGCTGTCCACTTGTAGGGAGTAGAGGTAGAGGCA
GTGAGGACTGGTGACTCCCTGTCCCAGCCCTGTGTCCTCAGCCCCACAACCACACTACCTTGAACAGGGT
GACGGTGGTGCTGTAGAAGAGGCTCAGGAGGAAGAGGACGATGAAGGTGGAGGCAGTGGTCCACAGGTTC
TCAAAGCCTTCCTCCTCAGCATTCACCTCCCCCTCTATGAAGGGACACAGAGAGGGAGGGTCAAGCCAAG
TCTTGGGGTGACTGAGCATTTGCATAAGGGTTGGGGTGCCCTGATGGTTCTGGAGAATCTTAGCGAGTAT
TGACTGGTCCCCTAATAGCTCAAGCTAGACGGAGGCACAGAACAGGCCAAGCTGTAGGACACACTAATTT
GGAGCCTTTGTTCCAGGAGCCCTGGGGCAGCAGAGCAAGCAAGACAGATGCAATGTATGGACCTGGGTAA
AGGCAGACTCAGGAACTGTGGCAAAATGATATTGGTGTGCTCTGGACTTTCCCTGAGCAGGTGGTGCCAT
CGGTGCTGTTTAGACTGAGAATTTAGAGCAGGTCTCCCTGTCTCTTGGGCTTTAGCAATAAGAACAGGGA
TACCAGAGGTTGTCCCTCCTTACTTCCTGAAGGACTCAGGCATCTCATGACTTTCTTTGATCAGAGTTCC
CGTGCGTTGCTTGTCCTACCCCTGCCAGCAATGTTGGGTCTTGGTACCAAGAGATAAACAGACAGATACC
TGAGGGAGGCTGTGGGACCAGGGCATTTATTGGGATCTCTGATCCCTGTAAAGATGAGCCCTTGGGGATA
TCTAAAGCCTAGGCCCCAAATGGATATGAATATGAAGAATGTCCAGGATATGAGCCCAAAGAGGCAGTGT
TATTGGGTGGTGACTCCTTACAGAGGCTCCTTGATGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
GTGTGTGTGTGTGTGTGTGTGTGTGTGGTTGGGGCATCTTTTGGACAACTGCATTATGTTTTCTGCATTT
TGAACTGGAGATCAGGGTGGGTGTGAGTGGGTTAGGCAATTTCACATTGGTCTTCTGAGGTGTTCTATTA
ATGGAGACACAGCCAAGGCTAACTTTCTGTCATGGGTGGTTAGTGGAGTATCTTCTGCCCCATGGTGTTG
AGACAGATAGGCATACTTTGTACCTGGGGTTATCTCTCCCAATCATAGAGTGGGCTCCTGAGGAGTATCA
GTGACCCAGCCTAGTGTAGGCTCTCTGAGCCGTGAGCCTTGGAGATTACAGCCCTGGTCAGAGTCCAGGT
GTATCTGTGTGCTATACCTGTTCACATGTTAGTGTACACATGTGAAGATGTTTCTGTACATGTGTGTATA
TGCATATCTTTGTATCTATATAGTTTATTTGTGTATATTTGCCTATAGTCTGTTTCTGTGTACACAGGAC
CATGCATATTGGTATCTGTTTCTGTGAGTGGTTGTGTGTCTATGCCTATTTGTCTCTGAAGGCGTTTGTA
AGTGTCCATGCATGTGTGTGCGCATGAACTCATGTGTATATATGTGTACCTTTGTCTGTGTGTCCATGAA
TACACATTCATGTATGTGTCTACACATGTGTACCTATATATCTATGTATAAATGCATGTGTGTCTATATA
TGTGCCTGAATGCTGCCTGCACCAGGTTAGGCAAGCTTTCAGCCATATATCTAGAAGTAGCTGGAGGAAT
CGCATGTTCATACTCTGTCTGTTGTAAGCCTGACTGAGTTCACACACAAGGAGGAAACTGGCAAGGTAGA
GGAAGGGCCAGGGTGGAGGCAAGTATGCAGGGTGTGTGCAGAGCAAGCAAAACACAACTGTCTCAGCAGG
CATGAGCATTGTATAATCAAAACCAGTGACGTTTGAATGGATTTTTTTTATTTCTAATTTTTATAAAATG
CAACATCTCACTCTGACATGGTTAGTTTGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGA
GCGCTAGCATGGTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTACAGTGTGGGT
TTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGTGGCAGGGCCTCGTGGCCTACAACACAGG
TATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGCTGTGGGTAAAGTAGAAGCCTGG
GGCCCCAGGCTCTGGCATCGGGGCACTGGTCACATACTTCTCTTGGGGCAAGAGTTGCCCTCTCTGAAGC
CACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAGGCAGGTGACTGTGGCTGACTCCCTCAGGT
TCAGTTGCTCACGAGCTGGTGGCAGCAGGTACACAGCAGGTGGATGTTTGTGCACCTCTGTAAATGAAGA
CAGTAGTGAGGATAGGGTGGAAGAGGACCTGCCCTCCCTATGAGGTACAGGAAGGGTCCTGCAATTGGAG
GGGAAGGGAAGGGGGGATACCTACCATTGGGTTTTGAGATGAATTTCTTCTGTGGTGAAGGCAGATCCCT
GTGAGTCACAGTACACACAAATTCCTTCCTGTTATTCCAGTCTTCCACACAAACACTAGCCACACCCTTA
GCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTTGGTTTCCAGTGGTTCACCACTTTGAG
AAGCCCAGGAGATATTCAGGGTTTCATAGGTTGCCAGGTTTGAGACCAGACAGGTCAGGTTAGCGGACTT
GCTGAGGAAGATGTCGGCAAAGGAGGGGGGGATGGTGAAGGTTAGGATGTCTGTGGAGGGACCTGCAGTC
AAGAGAACACTGTGTCAGGTTATGACATATCTAGTTGTGAAGTTGGAAAAAAGCTTAGTCTCCATCCCCC
AAACCTACTTAAGGGAAGATGCTGGTCCTTCCAGCTGATTCTCACCCCTCTACTGCCTATTGCTCATTCT
TGTCCTTGGCCACTCTGGGGAGCCAAAGTTCAAGGAGCAAATGACCATGTCTGGGTGGCATTGGCCATAA
TTGTAGGAGGACTTCCCTAATCTGGGAGGGCTAGGCATTGGGCTTAGCCCAGGCCACTCACTGGCAGCAC
ATGTGGAGGACACGTTCTTCAAGAAGGTGAGACCCCTGTGATCCACACGGCAGGTGTACACATTCAGGTT
CAGCCAGTCGATTTCAGAGATGGTAAGTGTGCTTATGACCTTGTAGGTTTGGGGTGTGGATCCTTTGTTC
TCGATGGTCACCGGATCTGTGGTGAAGCCAGATTCCACGAGCTTCCCATCCTTTAGCCAGGATACTGTGA
TCGGTTTTGGAGTGAAGTTCGTGGCCTCGCAGATGAGTTTAGACTTGCGTGGTGCAGGGCCAGAGAAGCC
ATCCCGTGGTGGGACGAACACATTTACATTGGGGTTCATCTCTGCGACAGCTGGAATGAAAGGTCAAGGT
GTGAGCTCAGCTTGGCCTAAGCCCCACCCCGCTGCTCCCTCTGGGCCATGCCTGGGCCTGGGCACCCCTG
CTGGGAGGGTTTGGTTCTTACCTGGAATGGGCACATGCAGATCTTTGTTTTTGCCTCCGTAGTGGATTTT
GCATACCAGGTATTCATCTGAACCTTCAAGGATGCTCTTGGGAGACAGCAACACCTGCGAGGTGGCTAGG
TACTTGCCCCCTGTCCTCAGTGTTGGGAAGGTTCTGATACCCTGGATGACTTCAGTGTTGTTCTGGTAGT
TCCAGGTGAAGGAAATGGTGCTGGGCAGGAAGTCCCGGGCCAGGCAGCCCATGGCCACCAGATTCTTATC
AGACAGGGGGCTCTCGCAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTC
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhimurium poxA mutant vaccine
12. IgM Fc receptor
  • Gene Name : IgM Fc receptor
  • Sequence Strain (Species/Organism) : Gallus gallus
  • NCBI Gene ID : 771853
  • Taxonomy ID : 9031
  • Chromosome No : 26
  • Gene Starting Position : 2406653
  • Gene Ending Position : 2408482
  • Gene Strand (Orientation) : -
  • Protein Name : Fc receptor, IgA, IgM, high affinity
  • DNA Sequence : Show Sequence 
    >gi|118135660:2406653-2408482 Gallus gallus chromosome 26, reference assembly (based on Gallus_gallus-2.1)
ATTAATACTTGGCTAACATTCCTACTTCAGCTATCAGGGACAGAAATGATGAGGGTGAAGAGTGAATTGC
ACCTGTGTCTCTGCATGCTGAGGTTCCTAGAACTGCAAGATAACCTTCAGAGCAAAGCACATCTCCATGC
TGCATGTGTTGTGCTCAGGATGCCATGTGCTGGTGTTTTCTATCATAAATTGTCCATGATATTTTCAAAC
AAACTTGAGCAGAAAGCTCAAATGTTTCTTGTAAAGCCACTACATTTATGCATGGGACAAAGGAGAGAAT
TTGAAGGGGGTATCCATGAAGGGATAGGGGGAAGAGTGATTAACAGAAGGAAAAGGGTTGATTTGTGTTT
CCAGCCAGCCATCGCCTACCCGTCTCGATGCGCAGTTTCATGGAGGTCGTGTGCTCCCTGTGCCTGTTCC
TGGCCACACACCAGTACCACCCCGCGTCCTCCTCCCGCAGGTGGCTCATCACCACGGTGTAGGTCCCACT
GTCCTGCTCACTGTTGCTGATCCATATGCGCCCTTTGTAGGACTCGTGCACAAACCCGTCTGTGTCTACC
AGCAGGGAGCAGCTGGCCTCCTGCCACCTGCACAGGTACTTGGTGGTGTAGCTGCCCACGGGGTTGTGGT
GGCACCTAAGGGACAGTGAGCCCCCCAGCCTGCCATGCAGAAACTTCAACCTCCGGGGCTGGGCAGGGAC
TGCAGGTAGAAGAAATCATGGTTATGTTTGATGTAGCTGCAATGAGCCAATGGGGCACAGCAGCCCCCAA
AACCCCCTCCTACCTGTGGTCACCTGCAGGGCCACCAGCTGCCAGCTGTCCCAGTCAGCTGGGCTGCCCA
TCCCACACTTGTACAGCCCTGCATCGTCCAACCTTAAATCATTTATCAGCACTTTGAATGCTCCAGAGCT
CTCCTGGGGGGTAATACAGATCCGTCCCTCGTAGCTCTTCCCCACATAGCCATCGGTGTCAGCAATGAGG
ATGCACCTGCTTCTCCCCACTCTGCACCATAGCCTCCTGGTGCCACCCCAGCTGTCCCCGGGTGGGCAGG
GGATGGTGATGGAGCCACGGAGCTCCCCGAGGTGCAGCTCAATTGGCCTCTGAGTGCCCACATCTGCAAA
GACAGAGCAGGTGAGTGCCACCACTGTGCCTGGTTGGGTGTCACCTCCTTTTGCATTGGTGCATTGGTCA
CATCAGTGGGTTTTATGCCCACGACTGCTTAAAGCAAAGGGTAGCAAAGGGGACCTGACACCCTCCCTGT
CCCATCACTGTACCTGCTGACACTGTCAGGTTCAGGCTGACGTAGAGGTCCCTGTTGGTGGGGCCAATGC
CACAGCGGTAGGTCCCTGTGTCACTTGTCTTCAGCTGTGTCATTGTAACCATGAAAGTGCCATGCTGGGG
GACGTCCTCCAGGGCCACTCGGCCCATGTGGTCTTTTGAGATGAAGTTGGTGGTGGAGATGATGGTGTAG
CAAATCCCATCTCTGTCCCTTTTACACCAAAACTTTCTGTCGTATTTGTTGGCAGGCGTGATGGAGTAGA
AGCACTGGTGGGTGATGGACCCTCCAACTGCGCCTGTCAAAAACCTGGGCCCATACAAGGTGCTTGAGGC
TGCCTCTTTGGGGAAGGAGGGAAGAAGCACATTAATCAGAGACGTGGACACCCCTGTGGATGGGGAATCT
TCCCCAACCCAAATGGGGTGCCACAGGCACAGGTCCACCAGCCCTGGCTGTCCTGCCCCATAGTGATGGG
GAGCAGCAAACCTTTGGTGCCTGCAGAGTTTTCTGGTGACTCACCTTGCAGCAAAGACACAAAGAGGAGG
AGAACCTCCA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Gallinarum relA/spoT mutant vaccine
13. IL-17
  • Gene Name : IL-17
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16171
  • NCBI Protein GI : 6754324
  • Genbank Accession : AC159614
  • Protein Accession : NP_034682
  • Taxonomy ID : 10090
  • Chromosome No : 1
  • Gene Starting Position : 20730904
  • Gene Ending Position : 20734495
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 17A
  • Protein pI : 9.13
  • Protein Weight : 16001.99
  • Protein Length : 158
  • Protein Note : Also known as Il17; Ctla8; IL-17; Ctla-8; IL-17A
  • DNA Sequence : Show Sequence 
    >gi|372099109:20730904-20734495 Mus musculus strain C57BL/6J chromosome 1, GRCm38 C57BL/6J
CATCCACCTCACACGAGGCACAAGTGCACCCAGCACCAGCTGATCAGGACGCGCAAACATGAGTCCAGGG
AGAGCTTCATCTGTGGTGAGTCCTGCACTAATGTACAAGGCGCTTGTTGATTGTGACCAGGTTGCGTTAT
TGCACGTGATATGAAATGCTCTATTCCAGCTGTTGGGGTCCATGAATCTGTACATGTAGAACTGGAAATG
AAACCTTTGGTAGATGTCGAAATCATTGTACAGCATTTTCAAGAACTGATAGGCATCAGTCAGAAAGATA
CGGTTAAAACTGAAGGGTTGCTGTGATGAAACTAACCCACTAAGAAATGTCACATGACGCTATGCAATGA
GAAAGACTGTCAAAATCTATTCTTCTGAGTGCAAGGGATTTGACGTCATTCTCTCATGTCAGTGCTCTTT
AATCCAGAATGAAGTGGGCTACTGTTCTTGCAGATTTGAAAGCTTTAGAAGCAAGTTTTCATCCATTGCT
TTATCCCAAAGTCTTAGGATATCCAAACTATTAGATACATGTATAAGTCTCCTTATGAATAAAGCTTTAT
ATATTTTAAATACTTAACCTAAAAAATATGCTCTGCACTCGTATTCTCATGATAGAATCAATGCATGCTA
AATTTAGAACCGGATAATATATACCTAAGAAAACATGAGGGAGGTTCAGTTTCTATCTGGAGGATAGCTG
GTGTACAAACACTGTTTCTAATTCTAACATGAGTGCCGACAAACAACGGGTAGGTTTTCTGCAGTCCTCT
CCTCCAAGGTACATGTTTGACTGTGCACGAGGTTTACAGAAGGAGTGACTAAGAACACTAGTGCATAAGG
ACAAGAAAATCGGGGTCATTCAGCAGCCCACTGACTCATGAGCTAACAGGCTGAGGAGATGGCAGAAGCT
GACATTTTTATTAGGACCACTGCTGTCTAAGACATCACATGATTGTCCTCAGTCACAAAAAGAATGTAAG
AAACTGGTTAAAAATAAAATAAATTCTACATTTTTTAGCATGATTTTGGCCATCTTAAAGTTTTTAAATT
CAGAATTACTTACATATTAGGAGAGATCTTTCCAAAATATCATATGTTTCCTACAGTTAGTTATATACTC
ATCACAATGAGTTTGTCAGAAAGAAAAGCAGATACTAATCCAATCCCCCATCACCTTATCTTTCCCTTCT
AGTCTCTGATGCTGTTGCTGCTGCTGAGCCTGGCGGCTACAGTGAAGGCAGCAGCGATCATCCCTCAAAG
CTCAGCGTGTCCAAACACTGAGGCCAAGGACTTCCTCCAGAATGTGAAGGTCAACCTCAAAGTCTTTAAC
TCCCTTGGCGCAAAAGTGAGCTCCAGAAGGCCCTCAGACTACCTCAACCGTTCCACGTCACCCTGGACTC
TCCAGTACGTAAGAACTCCAGACAAAATAATCTGTGTCCTTCCATTCTCTGATGCACCATGTATACCCTA
AACATGATTTTTCACTTTTTTCCTCTCAGTATCCTCAGAGACCTAGAAGGTCCATTGTGTTAAGCAAAGT
TTCCTCTACAGAGTTCTTGGATAGAGCAGGGAATACTTCCCCCTAACCTGTTATAGTGGTCAATAATGAG
AGAGATCAACCCAAATTATTGATAACCATGATTCTAGCATGAGAGCTCAAGTTGAATGCTGACTTTTTTG
GAAATCAGGATGTTTATGTTTCAACCAGACCTACAATTAAAGAGACTTGATGAAAAATAATTAATAACTA
ACTAGGTATTTTTGTGTCTGACCTGAAGAAGACATAGGAAAACAAAATTTGAATGGCTGGTAGAGGAAGC
ATCCTGCTGGATATGTTTTCTCCTTTGTGAGTTGGATAGACTTGACCTCCATCCCAAGCCCTTTTTTACT
TGACCTGTCTCTGGCATACACTATTTAGACCCCATAAAGGGCTCAGTACTTTGATCATTTGAGGTTATAT
GAATAAATTTCAATGGCAAGGAACCTGTGGAGCCACCTGTCTATAGGATTTTTCTCCTTTTTCCAAAACC
AAGGATAATTCAATCAATCAATTCAAAAATCTAAGTGAACTATTTATTCAGATATTGTAAAGGGACAAGA
AAGAGATTATAAAGGGACTTTTGTTGTTGCTTCTAGCTTTTCATATAACTTCCGTCCCAGGAAGTGTTCT
CAGATATAATTCTGATATTCCTTCTTTCAAAGACCAATGTCACCAAATCAGATGAACAGCCATTTTACTC
CTTGGTCATCTGTCCCTTTGTTGTCTCCTGTCAATCAAATGTACCCTTAAAATTTATCTTTTTTTTTTTT
ATGTTATCTACTTGATGTTAAGTTTCAATCCTCACCTCACTATGGTCTTTAGCTTATTCTGTTTATCTTT
CAAAAAGCATTCTTCTAAATCATTTCCTAAACTTTAAAATTCAAGTTTGTGAAGCTTCAGGTCCAGTTCC
ATGATGGAATGGGTGTCCATCATGTGGAAGACACTGGCTTTGATCCTTATTGCTGCGAAAGAGGAGGAGG
GAGAAAATGAGGAATCCGGGAGGAAAAGATAGAAAGGGGAGAATCTAAATTTTCAGACAAGCCAAAGTTA
GCAACTAACATGCAGAGCTTTGCAGTGCACATGTGTGTCACAGTGCGTTCTTCCTCCCTCCCCCGTTCCT
GCTTTTCCTTCCACTTAAGCAGGACTTCATTTCCTCCTGGCTTTTGTCTCCCCTGCAGCCGCAATGAAGA
CCCTGATAGATATCCCTCTGTGATCTGGGAAGCTCAGTGCCGCCACCAGCGCTGTGTCAATGCGGAGGGA
AAGCTGGACCACCACATGAATTCTGTTCTCATCCAGCAAGAGATCCTGGTCCTGAAGAGGGAGCCTGAGA
GCTGCCCCTTCACTTTCAGGGTCGAGAAGATGCTGGTGGGTGTGGGCTGCACCTGCGTGGCCTCGATTGT
CCGCCAGGCAGCCTAAACAGAGACCCGCGGCTGACCCCTAAGAAACCCCCACGTTTCTCAGCAAACTTAC
TTGCATTTTTAAAACAGTTCGTGCTATTGATTTTCAGCAAGGAATGTGGATTCAGAGGCAGATTCAGAAT
TGTCTGCCCTCCACAATGAAAAGAAGGTGTAAAGGGGTCCCAAACTGCTTCGTGTTTGTTTTTCTGTGGA
CTTTAAATTATTTGTGTATTTACAATATCCCAAGATAGCTTTGAAGCGTAACTTATTTTAATGAAGTATC
TACATTATTATTATGTTTCTTTCTGAAGAAGACAAAATTCAAGACTCAGAAATTTTATTATTTAAAAGGT
AAAGCCTATATTTATATGAGCTATTTATGAATCTATTTATTTTTCTTCAGTATTTGAAGTATTAAGAACA
TGATTTTCAGATCTACCTAGGGAAGTCCTAAGTAAGATTAAATATTAATGGAAATTTCAGCTTTACTATT
TGTTTATTTAAGGTTCTCTCCTCTGAATGGGGTGAAAACCAAACTTAGTTTTATGTTTAATAACTTTTTA
AATTATTGAAGATTCAAAAAATTGGATAATTTAGCTCCCTACTCTGTTTTAAAAAAAAATTGTAACAATA
TCACTGTAATAATAAAGTTTTG
  • Protein Sequence : Show Sequence 
    >gi|6754324|ref|NP_034682.1| interleukin-17A precursor [Mus musculus]
MSPGRASSVSLMLLLLLSLAATVKAAAIIPQSSACPNTEAKDFLQNVKVNLKVFNSLGAKVSSRRPSDYL
NRSTSPWTLHRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILVLKREPESCPFTFRVEK
MLVGVGCTCVASIVRQAA
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhimurium asd mutant vaccine , Salmonella Typhimurium asd/rfc mutant vaccine
14. IL-6
  • Gene Name : IL-6
  • Sequence Strain (Species/Organism) : Mus musculus
  • NCBI Gene ID : 16193
  • NCBI Protein GI : 13624311
  • Genbank Accession : AC112933
  • Protein Accession : NP_112445
  • Taxonomy ID : 10090
  • Chromosome No : 5
  • Gene Starting Position : 30013160
  • Gene Ending Position : 30019967
  • Gene Strand (Orientation) : +
  • Protein Name : interleukin 6
  • Protein pI : 7.5
  • Protein Weight : 23291.86
  • Protein Length : 211
  • Protein Note : Also known as Il-6
  • DNA Sequence : Show Sequence 
    >gi|372099105:30013160-30019967 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J
ACCAAGAACGATAGTCAATTCCAGAAACCGCTATGAAGTTCCTCTCTGCAAGTAAGTGAAGGCAGTTCCT
TGCCCTCTGGCGGAGCTATTGAGACTGTGAGAGAGGAGTGTGAGGCAGAGAGCCAGCATTGTGGGTTGGC
CAGCAGCCATCAGCTAGCAGCAGGCGCCCAACTGTGCTATCTGCTCACTTGCCGGTTTTCCCTTTTCTCC
ACGCAGGAGACTTCCATCCAGTTGCCTTCTTGGGACTGATGCTGGTGACAACCACGGCCTTCCCTACTTC
ACAAGTCCGGAGAGGAGACTTCACAGAGGATACCACTCCCAACAGACCTGTCTATACCACTTCACAAGTC
GGAGGCTTAATTACACATGTTCTCTGGGAAATCGTGGAAATGAGAAAAGAGGTGGGTAGGCTGTGAAACT
GATGAAGACCCAGTGTGGGCGTCCATTCATTCTCTTTGCTCTTGAATTAGAAATTCTCTGCTGGGATCTA
GGGCCCTTAGGATTTGAAGCTAAAGGTCAGACTAGACTGTGTTCTCTCTTTCTCACCTCTTTGCTGGTTT
TGAGTGGAGGTTGGGAAGGGGTTCCTTTCCTGTCTGGAAGATACAGAATGTGACTGCATTTCTAGAAAAT
TCACAGTGGGCCATTCTCTGTTCCAATTTAGCTATTGCTTCTTAGGTGGGGATTCCTTTTCCCTCATTAT
TCCTTTTTCCTTATCTCTTTGCCTTCATTTATCCCTTGAAGTCTACAAGGACCAGAAAGTGCTTTTTGGC
TGAATGTAGTAAGTTCTGTGTGTGTGAAATCAGGATGCTCTAGGGTCAGCCCAGATAAGATGCATATTGT
ATAGAGCCCAATAAAGTGCTAACACACACACACACACACACACACACACACAACCTAGATTAGGCTGGGC
ATGGTGGTGGATGCCTCCCAGCACTTGGGAGGCACACGCAGGAAGATCTCTGTGAGTTAGAGGCCAGCTT
GGTCTACATATTGAGTTCCAGGATATCCAAGACTACATTGAGAGATCCTTTCTCAAAAAAAAAAAAAAAA
AAAAAAAAACAACAACAACAAACAAACAAACAAACAAGAAAAAAAAAACCCAACCAACCCTACCTAGATT
AAAATATCTCCCATCTTTAAATGAAAAGTATTTTGACTCTGCTTGACAGAAAGGAAAAAAAAAATCACAA
ACAAGATTAATAGCCTAGGTCTGGGTGTGTAGAACCAACTTTGTAATCTTGGGAAAATCCTCAACTATTT
AGTACTCTTTATCTGCATAATTATGGAGTTACAGATAATGGCACAGACCCTTCCAGATGGAATATTCTGT
GACTTAAATTTATTTGCCCCAAGATATCATCAAAATTTCTGTCCTTTACCAAGTTCCTACTCTATGCTGG
GCAGTTTTTATCGTTGAATCCTTGAGACAAATGTATAAGAGGGACATCAATAATTCTTTATTGAAACATA
GGAAATACTTAGGAGAGTAACAAGCTTAAAGCCAAGGGACTGAGCCTAAGGGTGCATTTTTATTTATTGG
GCTGCTTAGTAGGGTTCTAAGAAGGTATAGCTTCCCTTTTCTGTGTCCTGGCTATGAAAGAGCATGACTT
GGCCCTCTAGTGGTGCTTGTTGTAAGAGGTGTTTAAGTGATTGCTCTGGTAATCTTTTGCAGTTGTGCAA
TGGCAATTCTGATTGTATGAACAACGATGATGCACTTGCAGAAAACAATCTGAAACTTCCAGAGATACAA
AGAAATGATGGATGCTACCAAACTGGATATAATCAGGTAGAAACTTGTCACGTTTACTTTCAACAATTCT
TCAAGGTTCTCTCTTGCACGTGATACCTGCATTTGTACAGTCCCAGTCAGGCAACAAAGATGGGGGGATA
CATCCAATACCACTTGGATGTAACTGGCCTGCAGTAGCTTGAATACAAAAGGAGATTGAGAGGTACCTCA
GCAGCTGCTAGGGTGAGGGAAAGTTTACCAACCAGTGCTATAAATGTTCACTCAATCTATGTCCATCAGG
AGCTAAATGTTTAAAGTCTCTATTCTCATCTTGGCTTTGAGGTGTTAGATGATTAAAAAAAAAACATTTT
TTTTGTCTAAAAAAGTGGAAGAGTAGATATAAAACATGTTATGTAAACGTCATGAGGAAAGTAACTTACA
GATTTAATTAAAAAGCAGTTTATTTAAACAGGTAAAGGCCCACTATGCTGGGATTCTTCACCACTGTTAA
ATGTTAGAAGTCATAGCAAGTTTAGCTAGTTCATGAGCCATCTCTTCTCTGGCTGCCCCTGGAAGGATCC
AGGCCTACACTCCCTACTGAGAACACTCTTCACACCCCTCTCCTTCCAGCCGGTGAGATAGTCTACATCA
GGTCTCAGGACAAGTCAGACAAGAACAAGAGTACAGGAAGGGCACTGCAGGATAGAAGGGAAGGAGTGAG
GGTCAGAAGTGGATTCCCACTGCCACACTCTGGGGTAGGGGGTGGGGGGATATTAAGTGGTGCCTCTCTC
TACTTGTCCAGATAATTCAGGAGGTCAGCTGATGCTGCCTGTTGTCTAATTCTGTAGAAGATACTGAACA
GCAGGAATCTTTTTTTCAATTTTTTATTAGATATTTTCTTCATTTACATTTCAAATGCTATCCCCACAGT
CCCCTATACCCTCCCCCCCCTCCCCGCTCCCTTACCCACCAACTCCCACTTCTTGGCCCTGGCATTCCCC
TGTACTGGGGCATATAAAGTTTGCAAGACCAAGGGGCCTCTATTCCCAATGATGGCTGACTAGGCCATCT
TCTGCTACATATGCAGCTAGAGATATGAGCTCTGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTAT
AGGGTTGCAGACCCCTTCAGCTCCTTGGGTACTTTCTCTAGCTCCTCCATTGGGGGCCCTGTGTTCCATC
CAATAGAAGACTGTGAGCATCCACTTTTTGCCAGGCATTGGCCTAGCCTCACACGAGACAGCTATATCAG
GGTCCTTTCAGCAAAATCTTGCTGGTATATGCAATACTGTCTGGGTTTGGTGGCTGATTATGGGATGGAT
CCCTAGGTGGGGTAGTCTCTGGATCTTCCATCCTTTTGTCTTAGCTCCAAACTTTGTCTCTGTAACTCCT
TCCATGGGTATTTTGTTCCCCATTCTAAGGAGGAATGAAGTATCCACATGTTGGTCTTCCTTCTTCTTGA
TTTTCTTGTGTTTTGGAAATTGTACCTTGGGTATTCTAAGTTTCTGGGCTAATATCCACTTATCAGTGAG
TGCATATCAAGTGACTTCTTTTGTGATTGGGTTACCTCACTAAGGATCATACTCTCCAGATACATCCATT
TGACCAAGAATTTCATAAATCCATTGTTTTTAATAGCTGAATAGTACTCCATTTGTAAATGTACCACATT
TTCTGTATCCATTCCTCTGTTGAGGGACATCTGGGTTCTTTCCAGCTTCTGGCTATTATGAATAAGGCTG
CTATGAACATAGTGGAGCATATGTCCTTATTACCAGTTGGAACATCTTCTGCGTATATGCCCAGAAGAGG
TATTGCTGGATCTTCCGGTAGTACTATGTCTAATTTTTTGAGGAACAGCCAGACTGATTTCCAGAGTGGT
TATACAAGCTTGCAATCCCAACAGCAATGGAGGAGTGTTCCTCTTTCTCCACATCCTTACCAGCATCTGC
TGTCACCTGAATTTTTGATCTTAGCCATTCTGACTGGTGTGAGGTGGAATCTCAGGGTTGTTTTGATTTG
CATTTCCCTGATGATTAAGGATGTTGAACATTTTTTCAAGTGCTTCTCAGCCATTCAGTATTCCTCAGTT
GAGAATTCTTTGTTTAGCTTTGTACCCCATTTTTAATGGGGTTATTTGAATTTCTGGAGTTCAGCTTCTT
GAGCTCTTTGTATATATTGGATATTAGTCCCCTATCAGATTTAGGATTGGTAAAAATCCTTTCCCAATCT
GTTGGTGGCCTTTTTGTCTTATTGACAGTGTCTTTTCCCTTACAGAAGCTTTGCAATTTTATGAGGTCCC
ATTTGTCGATTCTCGATTTTACTGTACAAGCCATTGCTGTTCTGTTCAGGAATTTTTCCCCTGTTCCCAT
ATCTACGAGGGTTTTTTTCCCACTTTCTCCTCTATAAATTTCAGTGTCTCTGGTTTTATGTGGAGTTCTT
TGATCCACTTAGACTTGAGCTTTGTACAAGGAGATAAGAATGGATCAATTCGGGCTGGAGAGATGGCTCA
GTGGTTAAGAGCACTGACTGCTCTTTCAGAAGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGTTCAT
AACCATCTATAATAAGATCTGATGTCCTTTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATGTA
ATAAATAAATAAAACTAGATTATTTAAAAAAAAAAAAAAAGAATGGATCAATTCACACTCTTCTACATGA
TAACCACCAGTTGAGCCCGCACCAATTGTTGAAAATGCTCTCTTTTTCCCACTGGATGGTTTTAGCTCCC
TTGTCAAAGATCAAGTGACCATAAGTGTGTGGGTTCATTTCTGTGTCTTCAATTGTGTTCCATTGATCTA
CCTGTCTGTTGCTGTATCAATACCATGCAGTTTTTTTTATCACAATTGCTCTGTAGTACAGCTTGAGGTC
AGGCATGGTGAGTCCACCAGAGGTTCTTTTATTGTTGAGAATAGTTTTTGCTATCCTAGTTTTTTTTTTT
TTTTTTTTTTGAACAGCAGGAATCTTATTCTCAAATTGAATCTATTCCTAGAAAGAACTGACTTCCTTTT
CCATTTTACTTATAGGAAATTTGCCTATTGAAAATTTCCTCTGGTCTTCTGGAGTACCATAGCTACCTGG
AGTACATGAAGAACAACTTAAAAGATAACAAGAAAGACAAAGCCAGAGTCCTTCAGAGAGATACAGAAAC
TCTAATTCATATCTTCAACCAAGAGGTGAGTGCTTCCCCATCTCTCATGCAGTGTGGGAAAGAGGACACC
CGGCACCTCAGGGTAGCGGCACTTTTTCCAGACAGCTGCTCAGAAGGGAGAGAGTCTGAACAACAGTTCT
TGATTATAAGGCCTTGCTTTGTTTTACTTTGGGGTTTTGTTTGAGGTTCTCTTTTGCAAAGAACATCAAT
ACCTGCTTTAAACTGTATTAATAGAATGTTACTAATTGCGTAAGAGGTATGAAAACTATGACAGCCATAC
ATAGTCACCCATTATGAGAGCACAGAGACAAAAGTGACTTTAATATTTAATCCTTGGCAAGTGACATTTT
TGTAACCAGAGTTCTAATGCAGAGAAGTAGCCAAAAGCTAAAATGTCAGGGAAGGAAGGGATCAAGGGCT
TCTGGCTACCATTAGCCAGAAGAATGGTAGATATGGAGGATTAATGACTCAGAGTGTGGGCGAACAAAGC
CAGATGCAATAAGAAGGGCCTGGAATGAAACCCTCTTGCTAAGGCTGCTTTTGCCACTTGTAGTTTCTTG
CCTTAAACCAGAGAGTTTCCCAATTTAATGTGCACAGGAACCACTTAGGGTCTTGTTCCAGCAGGGTCTT
ACTTAGGAGGTCTAGGAAGGGAACTAAGATTCATTTTTGTAAACAGCTGCTTGTTTATGACCCTGCTATA
TAGTATTCAGGCTCCTAAGTAGTGGGGCAAGCCTTCCAGTTAGTCTTCCCCATGCCAGTGGGCCCATGGA
AGGGTGTTTCCAGACTTCTTCATGCTACCACACTAAGAGACTCTCAACAGAGTCTGAATGGAAACCACGA
AGGAACACATTTGTTTTAGATTCCTCTGTGCCACCTTTACTGATGGGAGCTTCTGTTTTCCAGTAGAATA
CAGTATGTTAGCATGGATGCTTGGATAACAGACAAATAAGATGGTGGTGCTGACCTCTGGACGCTTACTC
TCTAGTGGCAGACAGAACAGTAAGGTTAGAATTCTGTTGCTATTAAAAAACTAATAATAATCACCTTGAA
AAAGAATGGAGTTGTTAGGCATGGGTCTCTCTCGAGTAAGCTTGGAACAAAAGCATCTCCCCTGGCTTGG
GTGAGTCAAAGCAGATGGACTTAGCTCGTCTCATTCATTCTAAATTAGAACTTCTTCCCACAGCCCAGAA
CACGCCACAAGAAAAAAAAAATGTGCAATATTTAACCAGTCTTTGTTTTTTCCTCCTTTAGGTAAAAGAT
TTACATAAAATAGTCCTTCCTACCCCAATTTCCAATGCTCTCCTAACAGATAAGCTGGAGTCACAGAAGG
AGTGGCTAAGGACCAAGACCATCCAATTCATCTTGAAATCACTTGAAGAATTTCTAAAAGTCACTTTGAG
ATCTACTCGGCAAACCTAGTGCGTTATGCCTAAGCATATCAGTTTGTGGACATTCCTCACTGTGGTCAGA
AAATATATCCTGTTGTCAGGTATCTGACTTATGTTGTTCTCTACGAAGAACTGACAATATGAATGTTGGG
ACACTATTTTAATTATTTTTAATTTATTGATAATTTAAATAAGTAAACTTTAAGTTAATTTATGATTGAT
ATTTATTATTTTTATGAAGTGTCACTTGAAATGTTATATGTTATAGTTTTGAAATGATAACCTAAAAATC
TATTTGATATAAATATTCTGTTACCTAGCCAGATGGTTTCTTGGAATGTATAAGTTTACCTCAATGAATT
GCTAATTTAAATATGTTTTTAAAGAAATCTTTGTGATGTATTTTTATAATGTTTAGACTGTCTTCAAACA
AATAAATTATATTATATT
  • Protein Sequence : Show Sequence 
    >gi|13624311|ref|NP_112445.1| interleukin-6 precursor [Mus musculus]
MKFLSARDFHPVAFLGLMLVTTTAFPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELC
NGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKAR
VLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQ
T
  • Molecule Role : Vaximmutor
  • Related Vaccine(s): Salmonella Typhimurium hfq mutant vaccine , Salmonella Typhimurium IppA/IppB/msbB , Salmonella Typhimurium IppB/msbB mutant vaccine
IV. Vaccine Information
1. Aro-deleted S. Typhi
a. Type:
Live, attenuated vaccine
b. Antigen
The antigen used was aro-deleted S. Typhi, a strain designated as CVD 908 (Tacket and Levine, 2007).
c. Gene Engineering of AroD
  • Type: Recombinant protein preparation
  • Description: Inactivation of AroD results in attenuation, and the deletions of both AroC and AroD provide a high level of safety against restoration of pathogenicity by recombination. An aroC/aroD-deleted derivative of S. Typhi strain Ty2 (the parent strain of Ty21a) is designated the strain "CVD 908" (Tacket and Levine, 2007).
  • Detailed Gene Information: Click here.
d. Gene Engineering of AroC
  • Type: Recombinant protein preparation
  • Description: Inactivation of AroC results in attenuation, and the deletions of both AroC and AroD provide a high level of safety against restoration of pathogenicity by recombination. An aroC/aroD-deleted derivative of S. Typhi strain Ty2 (the parent strain of Ty21a) is designated the strain "CVD 908" (Tacket and Levine, 2007).
  • Detailed Gene Information: Click here.
e. Preparation
CVD 908 was created by deleting both aroC and aroD, preventing restoration of pathogenicity by recombination (Tacket and Levine, 2007).
f. Virulence
After a single dose of the vaccine (5 × 10^4 or 5 × 10^5 cfu), vaccine bacteremia was not detected in serial blood cultures. Half of the subjects tested with this dose developed serum IgG anti–lipopolysaccharide antibodies and IgA anti-LPS antibody-secreting cells (Tacket and Levine, 2007).
g. Description
The aromatic metabolites, such as aroC and aroD, force Salmonella to be nutritionally dependent on p-aminobenzoic acid and 2,3-dihydroxy, both of which are not available in mammilian tissues, preventing proliferation in the host cells (Tacket and Levine, 2007).
h. Human Response
  • Vaccination Protocol: Each vaccine was ingested and contained 2.2 3 10^8 organisms of lyophilized vaccine. A second lower dose contained 5 3 10^7 viable organisms. Volunteers fasted for 90 minutes before and after vaccination. A placebo of sodium bicarbonate was also included (Tacket et al., 2000).
  • Immune Response: After a single oral dose, CVD 908-htrA stimulates vigor-
    ous mucosal, humoral, and cellular immune responses at levels (Tacket et al., 2000).
  • Side Effects: Two volunteers had diarrhea in the first 24 h after ingesting vaccine. Three volunteers had
    diarrhea that occurred later (Tacket et al., 2000).
  • Efficacy: Recipients of the high dose of CVD 908-htrA had a mean of 189 anti-LPS IgA ASC per 10^6 PBMC. Vigorous IgM and IgG ASC responses were detected among vaccinees. ASC-producing an-ti-H antigen IgA occurred in a majority of participants (Tacket et al., 2000).
  • Host Gene Response of human IgA
    • Gene Response: ASCs producing IgA anti-LPS were detected in 100% of recipients of the high-dose vaccine and in 92% of recipients of the lower-dose vaccine. ASCs producing IgA anti-H antigen occurred in 79% and 73% of high- and lower-dose vaccine recipients, respectively (Tacket and Levine, 2007).
    • Detailed Gene Information: Click here.
2. Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 4109.20)
a. Manufacturer:
Texas Vet Lab, Inc.
b. Vaccine Ontology ID:
VO_0001879
c. Type:
Inactivated or "killed" vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Cattle
3. Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 43T5.20)
a. Manufacturer:
Texas Vet Lab, Inc.
b. Vaccine Ontology ID:
VO_0001906
c. Type:
Inactivated or "killed" vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Cattle
4. Broilact Chick Salmonella vaccine
a. Tradename:
Broilact
b. Vaccine Ontology ID:
VO_0004140
c. Type:
Live, attenuated vaccine
d. Antigen
Broilact is a preparation of the complex mixture of bacteria that inhabit the gut of the healthy adult fowl. The Broilact bacteria are able to live normally in the gut and minimise its colonisation by undesirable and harmful bacteria (Game Bird Health - Broilact).
e. Preparation
Broilact consists of obligately and facultatively anaerobic chicken intestinal bacteria. (Schneitz and Renney, 2003)
f. Description
As a competitive exclusion product, Broilact substantially reduces colonization of the intestine of a host animal by Salmonella infantis (Schneitz and Renney, 2003).
g. Copper Pheasant Response
  • Host Strain: Copper Pheasant
  • Vaccination Protocol: Groups of 10 chicks were given 1.0 mg in a dose volume of 0.3 ml of Broilact through gavage (Schneitz and Renney, 2003).
  • Challenge Protocol: The challenge was created with a 24-hr brain-heart infusion broth culture of a nalidixic-acid-resistant derivative of Salmonella infantis. All birds were challenged 24 hours after initial dosage. Birds in trial 1 received 4.4 x 10^3 cfu each, while the birds in trials 2 and 3 received 4.3 x 10^3 cfu each (Schneitz and Renney, 2003).
  • Efficacy: Significant protection was induced by Broilact in all three trials (Schneitz and Renney, 2003).
h. Chicken Response
  • Host Strain: Isa Brown
  • Vaccination Protocol: Two trials of 20 chicks were inoculated through gavage at 20 times the normal dose of Broilact, 0.25 ml (Salvat et al., 1992).
  • Challenge Protocol: The challenge was given to the chicks through gavage using 0.1 ml of an 18-h diluted cuture. The first trial of chicks recieved 7.0 x 10^4 cfu per chick, while the second trial of chicks was inoculated with 5.7 x 10^3 cfu per chick (Salvat et al., 1992).
  • Efficacy: The protection factor (PF) was above 6 in the first trial and about 2.6 in the second trial at day 3. Protection at day 7 was extremely high (PF over 36 for trial 1 and P over 45 for trial 2) (Salvat et al., 1992).
5. CVD 908-htrA
a. Vaccine Ontology ID:
VO_0002865
b. Type:
Live, attenuated vaccine
c. Antigen
The antigen was htrA-deleted S.Typhi (Tacket and Levine, 2007).
d. Gene Engineering of AroC
  • Type: Recombinant protein preparation
  • Description: HtrA was deleted in Salmonella organisms, which created a mutant that was less virulent and more susceptible to oxidative killing. HtrA encodes a heat-shock protein in Salmonella (Tacket and Levine, 2007).

  • Detailed Gene Information: Click here.
e. Preparation
Formation of CVD 908-htrA was conducted from original CVC 908, and the vaccine created consisted of live lyophilized organisms in single-dose glass vials to be resuspended in buffer solution (Tacket and Levine, 2007).
f. Virulence
CVD 908-htrA retained the immunogenicity of the parent CVD 908, but no vaccine bacteremias were detected among people who received a single dose of 5 × 10^7, 5 × 10^8, or 5 × 10^9 cfu, despite surveillance for bacteremias after vaccination. The immune responses measured by seroconversion rates with IgG anti-LPS after vaccination with CVD 908 and CVD 908-htrA were very similar. Most volunteers developed ASC responses, regardless of the vaccine strain received. Some lymphoproliferative responses to flagella and particulate antigen after CVD 908-htrA vaccination were also observed (Tacket and Levine, 2007).
g. Human Response
  • Vaccination Protocol: In this study, 80 healthy adult outpatient volunteers were randomized to receive, with buffer, a single oral administration of high-dose CVD 908-htrA (4.5 × 108 cfu), lower-dose CVD 908-htrA (5 × 107 cfu), or placebo. On day 28, there was a crossover in which the volunteers who had ingested CVD 908-htrA vaccine on day 0 received placebo and those who had received placebo on day 0 received CVD 908-htrA vaccine in either the high or the lower dose (Tacket and Levine, 2007).
  • Side Effects: The incidence of adverse effects was the same in recipients of placebo, high-dose vaccine, or lower-dose vaccine after vaccination. Diarrhea, a potential concern observed in a small number of vaccine recipients in the uncontrolled, phase 1 study, occurred in 3 (4%) of 76 placebo recipients, 1 (3%) of 39 lower-dose vaccine recipients, and 4 (10%) of 39 high-dose vaccine recipients (a statistically insignificant difference). However, in a secondary analysis of the incidence of symptoms in the first 7 days after vaccination, recipients of the high-dose vaccine were found to be slightly more likely to have had diarrhea (8%) than were recipients of placebo (0%) (P = .04). No recipient of the lower-dose vaccine had diarrhea in the first 7 days after vaccination (Tacket and Levine, 2007).
  • Host Gene Response of human IgA
    • Gene Response: ASCs producing IgA anti-LPS were detected in 100% of recipients of the high-dose vaccine and in 92% of recipients of the lower-dose vaccine. ASCs producing IgA anti-H antigen occurred in 79% and 73% of high- and lower-dose vaccine recipients, respectively (Tacket and Levine, 2007).
    • Detailed Gene Information: Click here.
6. CVD 909
a. Vaccine Ontology ID:
VO_0004138
b. Type:
Live, attenuated vaccine
c. Status:
Licensed
d. Gene Engineering of HtrA
  • Type: Recombinant protein preparation
  • Description: CVD 909 was constructed by replacing the native promoter of tviA with the strong constitutive promoter Ptac (Wang et al., 2000).
  • Detailed Gene Information: Click here.
e. Preparation
S. Typhi CVD 909 was constructed to express the Vi-antigen. This involved a deletion in the promoter region of tviA and an insertion of the sacB-neo cassette, introduced into the chromosome of CVD 908-htrA by homologous recombination. The replacement of the sacB-neo cassette allowed the replacement of the promoter region with the Ptac promoter in a second homologous recombination event (Wang et al., 2000).
f. Human Response
  • Vaccination Protocol: 24 volunteers received a single oral dose of CVD 909 of 10^6–10^9 cfu, and 8 adults received 2 doses of 6 × 10^9 cfu per dose with buffer 14 days apart (Tacket and Levine, 2007).
  • Immune Response: All volunteers tested developed IgA anti-LPS ASCs. However, only 1 of the volunteers who ingested a single dose and only 1 of the 8 volunteers who ingested 2 doses developed serum IgG anti-Vi .However, IgA anti-Vi ASC responses were detected in a majority of volunteers who received 10^8–10^9 cfu of CVD 909. These Vi-specific ASC responses provided evidence that the Vi antigen was expressed and immunologically processed, even if serum antibody responses were realitvely small (Tacket and Levine, 2007).
  • Side Effects: Not noted.
g. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Three groups of 6-week-old BALB/c mice were immunized once intranasally with 10^10 CFU of either serovar Typhi strain CVD 908-htrA or CVD 909 or PBS (control) (Wang et al., 2000).
  • Immune Response: The mean titer of IgG anti-Vi was much higher in the mice that received CVD 909. However, GMTs of O antibody after immunization were quite similar in the two groups (CVD 909 and CDV 908-htrA) (Wang et al., 2000).
  • Side Effects: Not noted.
  • Challenge Protocol: Mice were challenged with wild-type serovar Typhi strain Ty2 thirty days after primary immunization (Wang et al., 2000).
  • Efficacy: A single mucosal dose of CVD 909 conferred significantly greater protection than CVD 908-htrA (Wang et al., 2000).
7. CVD 915
a. Vaccine Ontology ID:
VO_0004139
b. Type:
Live, attenuated vaccine
c. Antigen
The antigens used in the production of this vaccine are: Recombinant frag C, S. Typhi flagella, S.Typhi LPS, S.Typhi lysate, whole-cell heat phenolyzed S. Typhi, PHA, and BSA (Pasetti et al., 1999).
d. Gene Engineering of Frag C
e. Preparation
CVD 915 was manipulated with electroporation with plasmids pTETner15 and pcDNAtetc. guaBA was deleted from S. Typhi strain 915, which interrupts the biosynthesis of guanine nucleatides (Pasetti et al., 1999).
f. Virulence
High titers of IgG1, IgG2a, and IgG2b were observed against Frag C, elicited by the CVD 915. High titers of serum IgG antibody against S. Typhi flagella and LPS were observed in mice vaccinated with the CVS 915. Titers increased significantly following the booster dose.
g. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: BALB/c mice were injected immunized intranasally with 2x10^9 CFU of CVD 915 in a 30 microliter volume. Mice were boosted in the same manner 35 days later (Pasetti et al., 1999).
  • Immune Response: Immunization with CVD 915 elicited sensitized lymphoid cells that proliferated in the presence of Frag C. A strong immune response was observed (Pasetti et al., 1999).
  • Challenge Protocol: BALM/c mice were challenged with 50-100 lethal doses of tetanus toxin (Pasetti et al., 1999) .
8. Megan Egg
a. Tradename:
Megan Egg
b. Manufacturer:
Lohman Animal Health International
c. Vaccine Ontology ID:
VO_0000352
d. Type:
Live, attenuated vaccine
e. Status:
Licensed
f. Preparation
Megan Egg is a Salmonella vaccine that that consists of a double gene-deleted S. enterica serovar Typhimurium strain and is formulated fro immunization of pullets. Megan Egg contains the same Megan Vac1 organism but Megan Vac1 is used for younger birds (broilers) (Burns et al).
g. Immunization Route
Coarse spray application in the hatchery, or to birds on the barn floor or in cages, or in the drinking water
9. Megan Vac1
a. Tradename:
Megan Vac1
b. Manufacturer:
Lohman Animal Health International
c. Vaccine Ontology ID:
VO_0001022
d. Type:
Live, attenuated vaccine
e. Status:
Licensed
f. Host Species for Licensed Use:
Chicken
g. Immunization Route
Coarse spray application in the hatchery, or to birds on the barn floor or in cages, or in the drinking water
h. Description
Megan Vac1 is a Salmoneela vaccine that consists of a double gene-deleted S. enterica serovar Typhimurium strain and is designed for protection of broilers to reduce Salmonella contamination (Babu et al., 2004; Burns et al).
10. Newcastle Disease-Bronchitis Mass & Ark Types, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D7.10)
a. Manufacturer:
Lohmann Animal Health International
b. Vaccine Ontology ID:
VO_0002282
c. Type:
Inactivated or "killed" vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Chicken
11. Newcastle Disease-Bronchitis Mass & Ark Types, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D7.11)
a. Manufacturer:
Lohmann Animal Health International
b. Vaccine Ontology ID:
VO_0002283
c. Type:
Inactivated or "killed" vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Chicken
12. Newcastle-Bronchitis Mass Type, Killed Virus Vaccine-Salmonella Enteritidis Bacterin (USDA: 48D6.10)
a. Manufacturer:
Wyeth, Intervet Inc., Biomune Company
b. Vaccine Ontology ID:
VO_0002281
c. Type:
Inactivated or "killed" vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Chicken
13. S. typhi DnaJ Protein Vaccine
a. Vaccine Ontology ID:
VO_0004182
b. Type:
Subunit vaccine
c. Status:
Research
d. Gene Engineering of DnaJ
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
e. Adjuvant:
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunised on day 0 by injecting 40 μg DnaJ/mouse emulsified in Freund's complete adjuvant (100 μl) i.p. Subsequent booster injections were given by injecting 30 μg DnaJ/mouse emulsified in Freund's incomplete adjuvant i.p. on 7th and 28th days. The control mice received adjuvant alone (Sagi et al., 2006).
  • Challenge Protocol: After 15 days of last immunisation, i.e. 43rd day the mice were challenged with 10 MLD (1 × 10^5 cells) of S. Typhimurium i.p. The animals were observed daily for 30 days for morbidity and mortality (Sagi et al., 2006).
  • Efficacy: Immunisation of mice with DnaJ derived from Salmonella enterica subsp. enterica serovar Typhi str. CT18 was found to provide 70% protection against lethal challenge by S. typhimurium indicating the possible use of DnaJ as vaccine candidate against typhoid (Sagi et al., 2006).
14. S. typhi GroEL Protein Vaccine
a. Vaccine Ontology ID:
VO_0004183
b. Type:
Subunit vaccine
c. Status:
Research
d. Gene Engineering of GroEL
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
e. Adjuvant:
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice each were immunized i.m. with 10 μg of GroEL/mouse either alone or in combination with alum or CFA on 0, 7th, and 28th day as described earlier. As negative control, group of 10 mice was injected with endotoxin free water mixed with alum or CFA (Bansal et al., 2010).
  • Challenge Protocol: Two weeks after the last booster, all the groups were challenged with a lethal dose of 1 × 10^8 CFU/ml/mouse S. typhi Ty2 intraperitoneally (i.p.). The mice were observed daily for 30 days for any morbidity or mortality (Bansal et al., 2010).
  • Efficacy: Immunization of mice with GroEL + CFA provided 80% protection against lethal challenge by S. typhi in mice (Bansal et al., 2010).
15. S. typimurium Vi4072
a. Vaccine Ontology ID:
VO_0004186
b. Type:
Recombinant vector vaccine
c. Status:
Research
d. Gene Engineering of TviB
  • Type: Recombinant vector construction
  • Description:
  • Detailed Gene Information: Click here.
e. Vector:
Salmonella typhimurium χ4072, an attenuated Δcya Δcrp mutant (Cao et al., 1992).
f. Immunization Route
Orally
g. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunized orally with 5 x 10^7 recombinant Salmonella Vi4072. As controls, groups of mice were inoculated orally with 5 x 10^7 χ4072(pYA248) or BSG (Cao et al., 1992).
  • Challenge Protocol: At 60 days postimmunization, mice were challenged with virulent S. typhi Ty2 (Cao et al., 1992).
  • Efficacy: Immunization of mice with Vi4072 afforded complete protection against fatal infection with virulent S. typhi Ty2 (Cao et al., 1992).
  • Description: The viaB (tviB) gene coding for the Vi antigen of Salmonella typhi Ty2 was subcloned into expression vector pYA248. The recombinant plasmid was termed SMM202 and transformed into Salmonella typhimurium Vi4072, an attenuated ΔcyaΔcrp mutant (Cao et al., 1992).
16. Salmonella Choleraesuis Avirulent Live Culture Vaccine (USDA: 19A1.00)
a. Manufacturer:
Boehringer Ingelheim Vetmedica, Inc., Intervet Inc., Novartis Animal Health US, Inc., Arko Laboratories Ltd.
b. Vaccine Ontology ID:
VO_0001580
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Cattle
17. Salmonella Choleraesuis Avirulent Live Culture Vaccine (USDA: 19A1.01)
a. Manufacturer:
Boehringer Ingelheim Vetmedica, Inc., Arko Laboratories Ltd.
b. Vaccine Ontology ID:
VO_0001581
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Cattle
18. Salmonella DNA vaccine encoding SopB Protein
a. Vaccine Ontology ID:
VO_0004185
b. Type:
DNA vaccine
c. Status:
Research
d. Gene Engineering of sopB
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click here.
e. Vector:
pCI-TPA (Nagarajan et al., 2009)
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Host Strain: BALB/c
  • Vaccination Protocol: Six- to eight-week-old female BALB/c mice were immunized via i.p. route with 1 ml of PBS containing 10^3 CFU of ΔpmrG-HM-D strain per mouse and 10 μg of DNA vaccine plasmid in 50 μl volume of PBS in cohort of five mice. Booster doses were given on 7th and 14th day. Placebo control mice were injected with 10^3 CFU of ΔpmrG-HM-D strain per mouse and 10 μg of empty pCI-TPA vector (Nagarajan et al., 2009).
  • Challenge Protocol: 21 days after immunization, mice were challenged with 10^7 CFU/mouse of WT Salmonella orally (Nagarajan et al., 2009).
  • Efficacy: SopB DNA vaccine immunization reduced the bacterial burden of organs by about 5-fold on day 4 and day 8 after challenge with virulent Salmonella and proved to be a more efficient vaccination strategy than live attenuated bacteria alone (Nagarajan et al., 2009).
19. Salmonella Dublin Live Culture Vaccine (USDA: 19D1.R0)
a. Manufacturer:
Wyeth
b. Vaccine Ontology ID:
VO_0001582
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Cattle
20. Salmonella enterica fliC/guaB mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse, chicken
d. Gene Engineering of fliC
e. Gene Engineering of guaB from S. enteritidis str. P125109
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Persistence: A fliC/guaB mutant is attenuated in mice (Adriaensen et al., 2007).
  • Efficacy: A fliC/guaB mutant induces significant protection in mice from challenge with wild type S. enterica (Adriaensen et al., 2007).
  • Host Gene Response of IgG
    • Gene Response: Fifty-four days following initial oral immunization of BALB/c mice with approximately 108 CFU per mouse of, respectively, the S. enterica serovar Enteritidis ΔguaB and S. enterica serovar Enteritidis ΔguaB ΔfliC mutants, blood samples were collected from the tail arteries of five mice. Comparison between sera of mice immunized with S. enterica serovar Enteritidis ΔguaB and mice immunized with S. enterica serovar Enteritidis ΔguaB ΔfliC showed that, in both cases, comparable anti-LPS serum IgG responses were elicited (Adriaensen et al., 2007).
    • Detailed Gene Information: Click here.
h. Chicken Response
21. Salmonella Enteritidis guaBA/clpP mutant vaccine
a. Product Name:
CVD 1941
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of clpP
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Enteritidis (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
f. Gene Engineering of guaA
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Enteritidis (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
g. Gene Engineering of guaB from S. enteritidis str. P125109
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Enteritidis (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
h. Immunization Route
Oral immunization
i. Mouse Response
  • Persistence: A guaBA/clpP mutant is attenuated in mice (Tennant et al., 2011).
  • Efficacy: A guaBA/clpP mutant induces protection in mice from challenge with wild type Salmonella Enteritidis. The vaccine efficacy is 76% (Tennant et al., 2011).
  • Host Gene Response of IgG
    • Gene Response: Immune responses induced included high levels of serum IgG anti-lipopolysaccharide (LPS), with titers increasing progressively during the immunization schedule. For mice immunized with attenuated S. Enteritidis vaccine candidates, there was a trend of higher seroconversion rates after the first dose (26 days after immunization) in mice that received CVD 1941 than for those immunized with CVD 1943, which was statistically significant for anti-FliC (Tennant et al., 2011).
    • Detailed Gene Information: Click here.
22. Salmonella Gallinarum cbiA/cobS mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Chickens
d. Gene Engineering of cbiA
  • Type: Gene mutation
  • Description: This cbiA/cobS mutant is from Salmonella Gallinarum (Penha et al., 2010).
  • Detailed Gene Information: Click here.
e. Gene Engineering of cobS
  • Type: Gene mutation
  • Description: This cbiA/cobS mutant is from Salmonella Gallinarum (Penha et al., 2010).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intramuscular injection (i.m.)
g. Chicken Response
  • Persistence: A cbiA/cobS mutant is attenuated in chickens (Penha et al., 2010).
  • Efficacy: A cbiA/cobS mutant induces significant protection in chickens from challenge with wild type S. Gallinarum (Penha et al., 2010).
23. Salmonella Gallinarum cpxR/Ion mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of cpxR
e. Gene Engineering of lon
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Persistence: A cpxR/Ion mutant is highly attenuated in mice (Matsuda et al., 2010).
  • Efficacy: A cpxR/Ion mutant induces significant protection in mice from challenge with wild type S. Gallinarum (Matsuda et al., 2010).
24. Salmonella Gallinarum crp mutant vaccine
a. Vaccine Ontology ID:
VO_0002879
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of crp
  • Type: Gene mutation
  • Description: This crp mutant is from Salmonella Gallinarum (Rosu et al., 2007).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intramuscular injection (i.m.)
g. Mouse Response
  • Persistence: A crp mutant is attenuated in mice (Rosu et al., 2007).
  • Efficacy: A crp mutant induces significant protection in mice from challenge with wild type S. Gallinarum (Rosu et al., 2007).
25. Salmonella Gallinarum nuoG mutant vaccine
a. Vaccine Ontology ID:
VO_0002901
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Chickens
e. Gene Engineering of nuoG
f. Immunization Route
Intramuscular injection (i.m.)
g. Chicken Response
26. Salmonella Gallinarum relA/spoT mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Chickens
d. Gene Engineering of relA
  • Type: Gene mutation
  • Description: This relA/spoT mutant is from Salmonella Gallinarum (Park et al., 2010).
  • Detailed Gene Information: Click here.
e. Gene Engineering of spoT
  • Type: Gene mutation
  • Description: This relA/spoT mutant is from Salmonella Gallinarum (Park et al., 2010).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intramuscular injection (i.m.)
g. Chicken Response
  • Persistence: A relA/spoT mutant is attenuated in chickens (Park et al., 2010).
  • Efficacy: A relA/spoT mutant induces significant protection in chickens from challenge with wild type Salmonella Gallinarum (Park et al., 2010).
  • Host Gene Response of IgA Fc receptor
    • Gene Response: Serum IgG and IgA peaked at 3-4 wpi and was a significant antibody response against Salmonella vs. unimmunized chickens (Park et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG Fc fragment
    • Gene Response: Serum IgG and IgA peaked at 3-4 wpi and was a significant antibody response against Salmonella vs. unimmunized chickens (Park et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgM Fc receptor
    • Gene Response: The serum IgM levels against Salmonella in all chickens immunized with the mutant strains increased up to 3 weeks post infection (wpi), and subsequently decreased. The titers were significant vs. unimmunized chickens (Park et al., 2010).
    • Detailed Gene Information: Click here.
27. Salmonella IroN Protein Vaccine
a. Vaccine Ontology ID:
VO_0004184
b. Type:
Subunit vaccine
c. Status:
Research
d. Gene Engineering of IroN
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
e. Adjuvant:
f. Immunization Route
Intramuscular injection (i.m.)
g. Chicken Response
  • Host Strain: white leghorn
  • Vaccination Protocol: Ten 30-day-old specific-pathogen-free (SPF) white leghorn chickens were immunized intramuscularly with 0.5 ml of purified IroN (0.05 mg/ml), homogenized with Freund's incomplete adjuvant, and 10 control chickens were immunized intramuscularly with PBS homogenized with Freund's incomplete adjuvant. The immunized chickens were given an intramuscular booster injection 3 wk after the first immunization (Kaneshige et al., 2009).
  • Challenge Protocol: Three weeks after the second injection, the immunized chickens and controls were challenged intravenously with 10^8 CFU of Salmonella Enteritidis (Kaneshige et al., 2009).
  • Efficacy: The mortality rate of immunized chickens was 10%, while that of control chickens was 80% (Kaneshige et al., 2009).
28. Salmonella typhi aroA mutant vaccine
a. Vaccine Ontology ID:
VO_0002863
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of aroA
  • Type: Gene mutation
  • Description: This aroA was mutated from Salmonella typhi strain Ty2 (Dougan et al., 1987).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: An aroA mutant is attenuated in mice (Dougan et al., 1987).
  • Efficacy: An aroA mutant induced significant protection in mice from challenge with wild type S. typhi Ty2 (Dougan et al., 1987).
29. Salmonella Typhi galE mutant vaccine
a. Vaccine Ontology ID:
VO_0002883
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of galE
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
30. Salmonella Typhi phoP mutant vaccine
a. Vaccine Ontology ID:
VO_0002903
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of PhoP
  • Type: Gene mutation
  • Description: This phoP mutant is from Salmonella Typhi (Lee et al., 2007).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: A phoP mutant is attenuated in mice (Lee et al., 2007).
  • Efficacy: A phoP mutant provides significant protection in mice from challenge with wild type S. Typhi (Lee et al., 2007).
  • Host Gene Response of IgG
    • Gene Response: The serum titer of IgG was more than 1000-fold higher in mice immunized with a phoP mutant than in control mice. The mutant induced high titers of serum IgG against lipopolysaccharide of S. typhi at 8 weeks in the immunization schedule (Lee et al., 2007).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG2b
    • Gene Response: The titers of serum IgG2b increased significantly 8 weeks after inoculation in mice immunized with a phoP mutant as compared to unimmunized control mice (Lee et al., 2007).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighv1-9
    • Gene Response: Titers of serum IgG2a increased significantly 8 weeks after inoculation in mice immunized with a phoP mutant as compared to unimmunized control mice (Lee et al., 2007).
    • Detailed Gene Information: Click here.
31. Salmonella Typhimurium asd mutant vaccine
a. Vaccine Ontology ID:
VO_0002867
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of asd
  • Type: Gene mutation
  • Description: This asd mutant is from Salmonella Typhimurium (Piao et al., 2010).
  • Detailed Gene Information: Click here.
f. Immunization Route
Oral immunization
g. Mouse Response
  • Persistence: An asd mutant is attenuated in mice (Piao et al., 2010).
  • Efficacy: An asd mutant induces significant protection in mice from challenge with wild type Salmonella typhimurium (Piao et al., 2010).
  • Host Gene Response of IgA
    • Gene Response: Immunizing twice elicited significantly higher levels of IgA than immunizing once, and oral immunization was the most effective route in comparison to i.p. immunization. IgA levels for both oral and i.p. routes were significantly higher than unimmunized control mice titers. Sera was obtained two weeks after immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighg1
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG1 via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG1 antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighv1-9
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG2a via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG2a antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-17
    • Gene Response: Splenocytes from mice immunized via the oral route showed significantly high levels of IL-17 compared to non-immunized mice. Splenocytes were isolated and stimulated in vitro for 24 hours with wild type S. Typhimurium lysate. Immunizing twice was more effective than immunizing once (Piao et al., 2010).
    • Detailed Gene Information: Click here.
32. Salmonella Typhimurium asd/rfc mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of asd
  • Type: Gene mutation
  • Description: This asd/rfc mutant is from Salmonella Typhimurium (Piao et al., 2010).
  • Detailed Gene Information: Click here.
e. Gene Engineering of rfc
  • Type: Gene mutation
  • Description: This asd/rfc mutant is from Salmonella Typhimurium (Piao et al., 2010).
  • Detailed Gene Information: Click here.
f. Immunization Route
Oral immunization
g. Mouse Response
  • Persistence: An asd/rfc mutant is attenuated in mice (Piao et al., 2010).
  • Efficacy: An asd/rfc mutant induces significant protection in mice from challenge with wild type S. typhimurium (Piao et al., 2010).
  • Host Gene Response of IgA
    • Gene Response: Immunizing twice elicited significantly higher levels of IgA than immunizing once, and oral immunization was the most effective route in comparison to i.p. immunization. IgA levels for both oral and i.p. routes were significantly higher than unimmunized control mice titers. Sera was obtained two weeks after immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighg1
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG1 via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG1 antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of Ighv1-9
    • Gene Response: Immunizing twice instead of once induced significantly higher levels of IgG2a via the oral route compared to non-immunized mice. Sera was obtained 2 weeks after immunization. I.p. immunization induced higher levels of IgG2a antibodies than oral immunization (Piao et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-17
    • Gene Response: Splenocytes from mice immunized via the oral route showed significantly high levels of IL-17 compared to non-immunized mice. Splenocytes were isolated and stimulated in vitro for 24 hours with wild type S. Typhimurium lysate. Immunizing twice was more effective than immunizing once (Piao et al., 2010).
    • Detailed Gene Information: Click here.
33. Salmonella Typhimurium atpA mutant vaccine
a. Vaccine Ontology ID:
VO_0002869
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of atpA
f. Immunization Route
Intravenous injection (i.v.)
g. Mouse Response
34. Salmonella Typhimurium clpP mutant vaccine
a. Product Name:
CS2007
b. Vaccine Ontology ID:
VO_0002872
c. Type:
Live, attenuated vaccine
d. Status:
Research
e. Host Species as Laboratory Animal Model:
Mouse
f. Gene Engineering of clpP from S. typhimurium str. LT2
g. Immunization Route
Oral immunization
h. Mouse Response
  • Persistence: A clpP mutant (CS2007) causes hyperflagellation. These hyperflagellated bacteria are attenuated in mice (Matsui et al., 2003).
  • Efficacy: A clpP mutant elicited protection in mice from challenge with wild type S. typhimurium (Matsui et al., 2003).
  • Host Gene Response of IgA
    • Gene Response: Significant increases of lipopolysaccharide-specific immunoglobulin G (IgG) and secretory IgA were detected at week 4 and maintained until at least week 12 after inoculation in serum and bile. Testing before week 4 revealed undetectable or extremely low levels of antibodies, meaning the presence of much higher levels after week 4 were the result of upregulation of IgG and IgA (Matsui et al., 2003).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Significant increases of lipopolysaccharide-specific immunoglobulin G (IgG) and secretory IgA were detected at week 4 and maintained until at least week 12 after inoculation in serum and bile. Testing before week 4 revealed undetectable or extremely low levels of antibodies, meaning the presence of much higher levels after week 4 were the result of upregulation of IgG and IgA (Matsui et al., 2003).
    • Detailed Gene Information: Click here.
35. Salmonella Typhimurium cya/crp mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of crp from S. Typhimurium
e. Gene Engineering of cya
f. Immunization Route
Peroral Inoculation
g. Mouse Response
36. Salmonella Typhimurium guaBA/clpP mutant vaccine
a. Product Name:
CVD 1921
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of clpP from S. typhimurium str. LT2
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
f. Gene Engineering of guaA from S. typhimurium str. LT2
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
g. Gene Engineering of guaB
  • Type: Gene mutation
  • Description: This guaBA/clpP mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
h. Immunization Route
Oral immunization
i. Mouse Response
  • Persistence: A guaBA/clpP mutant is attenuated in mice (Tennant et al., 2011).
  • Efficacy: A guaBA/clpP mutant induces protection in mice from challenge with wild type Salmonella Typhimurium. The vaccine efficacy is 86% (Tennant et al., 2011).
  • Host Gene Response of IgG
    • Gene Response: Immune responses induced included high levels of serum IgG anti-lipopolysaccharide (LPS), with titers increasing progressively during the immunization schedule. Mice immunized with attenuated S. Typhimurium construct CVD 1921 had excellent seroconversion rates after the second immunization and mounted robust antibody responses against both target antigens (LPS and FliC) (Tennant et al., 2011).
    • Detailed Gene Information: Click here.
37. Salmonella Typhimurium guaBA/clpP/fliD mutant vaccine
a. Product Name:
CVD 1923
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of clpP from S. typhimurium str. LT2
  • Type: Gene mutation
  • Description: This guaBA/clpP/fliD mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
f. Gene Engineering of fliD
  • Type: Gene mutation
  • Description: This guaBA/clpP/fliD mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
g. Gene Engineering of guaA from S. typhimurium str. LT2
  • Type: Gene mutation
  • Description: This guaBA/clpP/fliD mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
h. Gene Engineering of guaB
  • Type: Gene mutation
  • Description: This guaBA/clpP/fliD mutant is from Salmonella Typhimurium (Tennant et al., 2011).
  • Detailed Gene Information: Click here.
i. Immunization Route
Oral immunization
j. Mouse Response
  • Persistence: A guaBA/clpP/fliD mutant is attenuated in mice (Tennant et al., 2011).
  • Efficacy: A guaBA/clpP/fliD mutant induces protection in mice from challenge with wild type Salmonella Typhimurium. The vaccine efficacy is 80% (Tennant et al., 2011).
  • Host Gene Response of IgG
    • Gene Response: Immune responses induced included high levels of serum IgG anti-lipopolysaccharide (LPS), with titers increasing progressively during the immunization schedule. Mice immunized with attenuated S. Typhimurium construct CVD 1923 had excellent seroconversion rates after the second immunization and mounted robust antibody responses against both target antigens (LPS and FliC) (Tennant et al., 2011).
    • Detailed Gene Information: Click here.
38. Salmonella Typhimurium gyrA/cpxA/rpoB mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Pigs
d. Gene Engineering of cpxA
  • Type: Gene mutation
  • Description: This gyrA/cpxA/rpoB mutant is from Salmonella Typhimurium (Roesler et al., 2004).
  • Detailed Gene Information: Click here.
e. Gene Engineering of gyrA
  • Type: Gene mutation
  • Description: This gyrA/cpxA/rpoB mutant is from Salmonella Typhimurium (Roesler et al., 2004).
  • Detailed Gene Information: Click here.
f. Gene Engineering of rpoB
  • Type: Gene mutation
  • Description: This gyrA/cpxA/rpoB mutant is from Salmonella Typhimurium (Roesler et al., 2004).
  • Detailed Gene Information: Click here.
g. Immunization Route
Oral immunization
h. Pig Response
  • Persistence: A gyrA/cpxA/rpoB mutant was attenuated in pigs (Roesler et al., 2004).
  • Efficacy: A gyrA/cpxA/rpoB mutant protected pigs against challenge with wild type Salmonella Typhimurium (Roesler et al., 2004).
  • Host Gene Response of IgM
    • Gene Response: A significant discrimination between vaccinated and non-vaccinated pigs was observed when IgM was measured after challenge, but here, the non-immunized animals had an elevated humoral antibody response after challenge with wild type Salmonella. The first significant difference was seen on day 35 after vaccination (Roesler et al., 2004).
    • Detailed Gene Information: Click here.
39. Salmonella Typhimurium hfq mutant vaccine
a. Vaccine Ontology ID:
VO_0002892
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of hfq
  • Type: Gene mutation
  • Description: This hfq mutant is from Salmonella Typhimurium (Allam et al., 2011).
  • Detailed Gene Information: Click here.
f. Immunization Route
Oral immunization
g. Mouse Response
  • Persistence: An hfq mutant is attenuated in mice (Allam et al., 2011).
  • Efficacy: An hfq mutant efficiently protects mice against subsequent oral challenge with virulent strain of Salmonella Typhimurium (Allam et al., 2011).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: Vaccinated mice had significantly higher serum IFN-γ and IL-6 than the PBS administered mice. The serum IL-6 and IFN-γ levels of vaccinated mice further increased upon challenge while remaining lower than that of unvaccinated challenged mice. Serum was collected 7 days after vaccination from unchallenged mice or 7 days after challenge from challenged mice (Allam et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgA
    • Gene Response: Mucosal IgA and serum IgG levels significantly increased in vaccinated mice compared to naive mice. The antibody titers remained high in case of mice vaccinated and challenged compared with the unvaccinated and challenged mice. The elicited humoral immune response remained 4 weeks after immunization with mutant strain (Allam et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Mucosal IgA and serum IgG levels significantly increased in vaccinated mice compared to naive mice. The antibody titers remained high in case of mice vaccinated and challenged compared with the unvaccinated and challenged mice. The elicited humoral immune response remained 4 weeks after immunization with mutant strain (Allam et al., 2011).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-6
    • Gene Response: Vaccinated mice had significantly higher serum IFN-γ and IL-6 than the PBS administered mice. The serum IL-6 and IFN-γ levels of vaccinated mice further increased upon challenge while remaining lower than that of unvaccinated challenged mice. Serum was collected 7 days after vaccination from unchallenged mice or 7 days after challenge from challenged mice (Allam et al., 2011).
    • Detailed Gene Information: Click here.
40. Salmonella Typhimurium htrA mutant vaccine
a. Vaccine Ontology ID:
VO_0002893
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of htrA
f. Immunization Route
Intravenous injection (i.v.)
g. Mouse Response
41. Salmonella Typhimurium IppA/IppB/msbB
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of lppA
  • Type: Gene mutation
  • Description: This IppA/IppB/msbB mutant is from Salmonella Typhimurium (Liu et al., 2008).
  • Detailed Gene Information: Click here.
e. Gene Engineering of lppB
  • Type: Gene mutation
  • Description: This IppA/IppB/msbB mutant is from Salmonella Typhimurium (Liu et al., 2008).
  • Detailed Gene Information: Click here.
f. Gene Engineering of msbB
  • Type: Gene mutation
  • Description: This IppA/IppB/msbB mutant is from Salmonella Typhimurium (Liu et al., 2008).
  • Detailed Gene Information: Click here.
g. Immunization Route
Intraperitoneal injection (i.p.)
h. Mouse Response
  • Persistence: An IppA/IppB/msbB mutant is attenuated in mice (Liu et al., 2008).
  • Efficacy: An IppA/IppB/msbB mutant provides significant protection in mice from challenge with wild type S. Typhimurium (Liu et al., 2008).
  • Host Gene Response of Ighg1
    • Gene Response: This mutant elicited a significantly greater serum IgG1 response compared to infection with the wild type on days 2, 5, and 7 post infection. Peak levels were reached five days after infection (Liu et al., 2008).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-6
    • Gene Response: Culture supernatants from T cells infected with the lppB/msbB mutant contained significantly higher levels of IL-6 than did supernatants from T cells that were infected with WT S. Typhimurium (Liu et al., 2008).
    • Detailed Gene Information: Click here.
42. Salmonella Typhimurium IppB/msbB mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of lppB
  • Type: Gene mutation
  • Description: This IppB/msbB mutant is from Salmonella Typhimurium (Liu et al., 2008).
  • Detailed Gene Information: Click here.
e. Gene Engineering of msbB
  • Type: Gene mutation
  • Description: This IppB/msbB mutant is from Salmonella Typhimurium (Liu et al., 2008).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: An IppB/msbB mutant is attenuated in mice (Liu et al., 2008).
  • Efficacy: An IppB/msbB mutant provides significant protection from challenge with wild type S. Typhimurium (Liu et al., 2008).
  • Host Gene Response of Ighg1
    • Gene Response: This mutant elicited a significantly greater serum IgG1 response compared to infection with the wild type on day 14 after vaccination. Peak levels were reached five days after infection (Liu et al., 2008).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IL-6
    • Gene Response: Culture supernatants from T cells infected with the lppB/msbB mutant contained significantly higher levels of IL-6 than did supernatants from T cells that were infected with WT S. Typhimurium (Liu et al., 2008).
    • Detailed Gene Information: Click here.
43. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.00)
a. Manufacturer:
Wyeth
b. Vaccine Ontology ID:
VO_0001727
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Chicken
44. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.01)
a. Manufacturer:
Lohmann Animal Health International
b. Vaccine Ontology ID:
VO_0001728
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Chicken
45. Salmonella Typhimurium Live Culture Vaccine (USDA: 19C1.02)
a. Manufacturer:
Lohmann Animal Health International, Biomune Company
b. Vaccine Ontology ID:
VO_0001729
c. Type:
Live vaccine
d. Status:
Licensed
e. Location Licensed:
USA
f. Host Species for Licensed Use:
Chicken
46. Salmonella Typhimurium ompD/nmpC mutant vaccine
a. Vaccine Ontology ID:
VO_0002900
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Pig
e. Gene Engineering of NmpC
  • Type: Gene mutation
  • Description: This ompD/nmpC mutant is from Salmonella Typhimurium (Selke et al., 2007).
  • Detailed Gene Information: Click here.
f. Immunization Route
Oral immunization
g. Pig Response
  • Persistence: An ompD/nmpc mutant is attenuated in pigs (Selke et al., 2007).
  • Efficacy: In a pig infection experiment including two oral immunizations with SalmoporcDeltaompD (OmpD, nmpC) and challenge with a multiresistant S. enterica serovar Typhimurium DT104 clinical isolate, we confirmed the protective efficacy of SalmoporcDeltaompD (Selke et al., 2007).
47. Salmonella Typhimurium ompR mutant vaccine
a. Vaccine Ontology ID:
VO_0002902
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of ompR
f. Immunization Route
Intravenous injection (i.v.)
g. Mouse Response
  • Persistence: An ompR mutant is attenuated in mice after oral challenge (Dorman et al., 1989).
  • Efficacy: A ompR mutant conferred significant protection in mice from challenge with wild type Salmonella Typhimurium (Dorman et al., 1989).
48. Salmonella Typhimurium poxA mutant vaccine
a. Vaccine Ontology ID:
VO_0002904
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of poxA
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: A poxA mutant is attenuated in mice (Kaniga et al., 1998).
  • Efficacy: A poxA mutant provided significant protection in mice from challenge with wild type Salmonella Typhimurium (Kaniga et al., 1998).
  • Host Gene Response of IgA
    • Gene Response: Sera were collected 29 days after a single oral immunization and 16 days post challenge. High IgG, IgA, and, to a lesser extent, IgM titers were detected in orally immunized animals, indicating that the ΔpoxA270 mutant derivative of S. typhimurium was very immunogenic in mice. This was more than negative control serum collected from non immunized mice (Kaniga et al., 1998).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Sera were collected 29 days after a single oral immunization and 16 days post challenge. High IgG, IgA, and, to a lesser extent, IgM titers were detected in orally immunized animals, indicating that the ΔpoxA270 mutant derivative of S. typhimurium was very immunogenic in mice. This was significantly more than negative control serum collected from non immunized mice (Kaniga et al., 1998).
    • Detailed Gene Information: Click here.
49. Salmonella Typhimurium rpoS mutant vaccine
a. Vaccine Ontology ID:
VO_0002908
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of rpoS
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: An rpoS mutant is attenuated in mice (Coynault et al., 1996).
  • Efficacy: An rpoS mutant protects mice from challenge with wild type Salmonella typhimurium after a single dose (Coynault et al., 1996).
50. Salmonella Typhimurium ruvB mutant vaccine
a. Vaccine Ontology ID:
VO_0002909
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of ruvB
  • Type: Gene mutation
  • Description: This ruvB mutant is from Salmonella Typhimurium (Choi et al., 2010).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: A ruvB mutant is attenuated in mice (Choi et al., 2010).
  • Efficacy: A ruvB mutant provides protection in mice from challenge with wild type Salmonella Typhimurium (Choi et al., 2010).
  • Host Gene Response of Ifng (Interferon gamma)
    • Gene Response: The vaccine induced significant production of IFN-γ on days 18 and 22 after vaccination while the control mice (non vaccinated) showed no change in the level of IFN-γ (Choi et al., 2010).
    • Detailed Gene Information: Click here.
  • Host Gene Response of IgG
    • Gene Response: Total IgG levels were significantly increased in vaccinated mice compared to the non-vaccinated group at both day 18 and day 22 (Choi et al., 2010).
    • Detailed Gene Information: Click here.
51. Salmonella Typhimurium surA mutant vaccine
a. Vaccine Ontology ID:
VO_0002911
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of surA
f. Immunization Route
Intravenous injection (i.v.)
g. Mouse Response
52. Salmonella Typhimurium tolA mutant vaccine
a. Vaccine Ontology ID:
VO_0002912
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of tolA
f. Immunization Route
Intravenous injection (i.v.)
g. Mouse Response
53. Salmonella Typhimurium trxA mutant vaccine
a. Vaccine Ontology ID:
VO_0002913
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of trxA
  • Type: Gene mutation
  • Description: A trxA mutant is attenuated in mice and induced significant protection from challenge with wild type S. Typhimurium (Peters et al., 2010).
  • Detailed Gene Information: Click here.
f. Immunization Route
Intravenous injection (i.v.)
g. Mouse Response
  • Persistence: A trxA mutant is attenuated in mice (Peters et al., 2010).
  • Efficacy: A trxA mutant induces protection in mice from challenge with wild type Salmonella Typhimurium (Peters et al., 2010).
54. Salmonella Typhimurium wecA mutant vaccine
a. Vaccine Ontology ID:
VO_0002914
b. Type:
Live, attenuated vaccine
c. Status:
Research
d. Host Species as Laboratory Animal Model:
Mouse
e. Gene Engineering of wecA
f. Immunization Route
Intraperitoneal injection (i.p.)
g. Mouse Response
  • Persistence: A wecA mutant (DSM645) is attenuated in C57BL/6J mice through i.p. and oral immunization (Gilbreath et al., 2011).
  • Efficacy: A wecA mutant (DSM645) protects against challenge with S. typhimurium strains TML and SL1344 30 days after immunization (Gilbreath et al., 2011).
55. Salmonella Typhimurium znuABC mutant vaccine
a. Type:
Live, attenuated vaccine
b. Status:
Research
c. Host Species as Laboratory Animal Model:
Mouse
d. Gene Engineering of znuA
e. Gene Engineering of znuB
f. Gene Engineering of znuC
g. Immunization Route
Oral immunization
h. Mouse Response
56. Salmonella vacT
a. Tradename:
vacT
b. Vaccine Ontology ID:
VO_0004142
c. Type:
Live, attenuated vaccine
d. Antigen
Salmonella vacT is made of S. Typhimurium (Methner et al., 1997).
e. Chicken Response
  • Vaccination Protocol: A group of one-day-old SPF chicks were innoculated orally with 0.1 ml of a vacT culture such that each bird received between 6 x 10^8 and 1 x 10^9 cfu each (Methner et al., 1997).
  • Challenge Protocol: The birds were challenged 24 h later orally with 0.1 ml of nalidixic acid-resistant Salmonella derivative diluted in PBS such that each bird recieved 10^4 cfu (Methner et al., 1997).
  • Efficacy: A significant amount of S. Typhimurium 421 was found in the livers and the caeca of the birds days after the challenge (Methner et al., 1997).
57. Typherix
a. Tradename:
Typherix
b. Manufacturer:
GlaxoSmithKline
c. Vaccine Ontology ID:
VO_0010744
d. Type:
Subunit vaccine
e. Status:
Licensed
f. Location Licensed:
Canada
g. Host Species for Licensed Use:
Human
h. Preservative:
Phenol
i. Allergen:
Latex in plunger stopper of pre-filled syringes
j. Immunization Route
Intramuscular injection (i.m.)
k. Description
Products: Polysaccharide.
58. Typhim Vi
a. Product Name:
Typhoid Vi Polysaccharide Vaccine
b. Tradename:
Typhim Vi
c. Manufacturer:
Sanofi Pasteur SA
d. Vaccine Ontology ID:
VO_0000104
e. CDC CVX code:
101
f. Type:
Subunit vaccine
g. Status:
Licensed
h. Location Licensed:
USA (License #1724)
i. Host Species for Licensed Use:
Human
j. Antigen
The Vi-antigen is a polysaccharide capsule found only in S. enterica serovars Dublin, Typhi, and Paratyphi C The Vi-antigen is a polysaccharide capsule found only in S. enterica serovars Dublin, Typhi, and Paratyphi C (Cheminay and Hensel, 2007).
k. Preservative:
Phenol
l. Immunization Route
Intramuscular injection (i.m.)
m. Storage
Store at 2° to 8°C (35° to 46°F). DO NOT FREEZE.
n . Approved Age for Licensed Use
Two years of age and older.
o. Contraindication
TYPHIM Vi VACCINE IS CONTRAINDICATED IN PATIENTS WITH A HISTORY OF HYPERSENSITIVITY TO ANY COMPONENT OF THIS VACCINE (Typhim Vi).
p. Description
Contains the cell surface Vi polysaccharide extracted from Salmonella enterica serovar Typhi, S typhi Ty2 strain. The organism is grown in a semi-synthetic medium without animal proteins. The capsular polysaccharide is precipitated from the concentrated culture supernatant by the addition of hexadecyltrimethylammonium bromide and the product is purified by differential centrifugation and precipitation (Typhim Vi).
q. Human Response
  • Side Effects: The most common side effects reported are: injection site reactions, fever, headache, nausea, and malaise (Typhim Vi).
  • Efficacy: The overall protective efficacy of Typhim Vi vaccine was 74% confirmed by a blood culture of confirmed cases of typhoid fever during 20 months of post-vaccination follow-up (Typhim Vi).
59. ViVaxim
a. Product Name:
Combined Purified Vi Polysaccharide Typhoid and Inactivated Hepatitis A Vaccine
b. Tradename:
ViVaxim
c. Manufacturer:
Sanofi Pasteur Ltd
d. Vaccine Ontology ID:
VO_0010748
e. Type:
Subunit vaccine + Inactivated or "killed" vaccine
f. Status:
Licensed
g. Location Licensed:
Canada
h. Host Species for Licensed Use:
Human
i. Adjuvant:
j. Preservative:
2 phenoxy ethanol
k. Allergen:
Neomycin
l. Immunization Route
Intramuscular injection (i.m.)
m. Storage
Store at 2° to 8°C (35° to 46°F).
n . Approved Age for Licensed Use
16 years of age and older.
o. Description
Products: Polysaccharide + killed virus. Other components: Formaldehyde, Polysorbate 80.
60. Vivotif
a. Product Name:
Typhoid Vaccine Live Oral Ty21a
b. Tradename:
Vivotif
c. Manufacturer:
Berna Biotech, Ltd
d. Vaccine Ontology ID:
VO_0000120
e. CDC CVX code:
25
f. Type:
Live, attenuated vaccine
g. Status:
Licensed
h. Location Licensed:
USA (License # 1632)
i. Host Species for Licensed Use:
Human
j. Antigen
The antigen is live, attenuated strain Ty21a (Levine et al., 2007).


k. Preparation
Ty21a in enteric-coated capsules was created with 2 × 10^9–5 × 10^9 cfu of Ty21 (Levine et al., 2007).
l. Immunization Route
Oral
m. Storage
between 2 °C and 8 °C (35.6 °F– 46.4 °F).
n . Approved Age for Licensed Use
6 years of age and older.
o. Contraindication
Hypersensitivity to any component of the vaccine or the enteric-coated capsule (FDA: Vivotif).
p. Description
Indication: VIVOTIF is used for immunization of adults and children greater than 6 years of age against disease caused by Salmonella typhi (FDA: Vivotif).
q. Human Response
  • Vaccination Protocol: Two trials were completed with Ty21a: In the first, Participants were randomly allocated to receive, 1 week apart, either 2 doses of Ty21a in enteric-coated capsules, 1 dose of Ty21a and 1 dose of placebo, or 2 doses of placebo. In the second, participants were randomly allocated to receive 3 doses of vaccine in 1 of 2 different formulations or placebo. One formulation of Ty21a was enteric-coated capsules, and the other was a triad of gelatin capsules, with 1 containing lyophilized vaccine and the other 2 containing NaHCO3 buffer.
  • Persistence: Not noted.
  • Immune Response: Doses of Ty21a in capsules stimulates a form of antibody-dependent cellular cytotoxicity, in which IgA antibodies provide the specificity for cells to kill Salmonella organisms. Ty21a stimulates strong classical cell-mediated immune responses involving specific T cells that are believed to function through different effector mechanisms, such as production of IFN-gamma and other cytokines when stimulated by inactivated whole bacilli and purified S. Typhi flagella and the appearance of cytotoxic T cells that kill target cells infected with S. Typhi (Levine et al., 2007). Unless a complete immunization schedule is followed, an optimum immune response may not be achieved (FDA: Vivotif).
  • Side Effects: The most common side effects reported were: abdominal pain, nausea, headache, fever, diarrhea, vomiting and skin rash (FDA: Vivotif).
  • Efficacy: Results from clinical studies indicate that adults and children greater than 6 years of age may be protected against typhoid fever following the oral ingestion of 4 doses of VIVOTIF (FDA: Vivotif).
61. Vivotif L
a. Product Name:
Typhoid Vaccine Live Oral Attenuated Ty21a
b. Tradename:
Vivotif L
c. Manufacturer:
Berna Biotech
d. Vaccine Ontology ID:
VO_0011556
e. Type:
Live vaccine
f. Status:
Licensed
g. Location Licensed:
Canada
h. Host Species for Licensed Use:
Human
i. Immunization Route
Oral
j. Storage
Store between 2 and 8°C. Store in a dry place and protect from light.
k . Approved Age for Licensed Use
3 years of age and older.
l. Description
Products: Live virus. Other components: Lactose, Aspartame.
62. Zoosaloral H.
a. Tradename:
Zoosaloral H.
b. Vaccine Ontology ID:
VO_0004141
c. Type:
Live, attenuated vaccine
d. Antigen
The antigen is a strain of S. Typhimurium called Zoosaloral H.
e. Preparation
This live vaccine strain is derived by chemical mutagenesis from a virulent avian S. Typhimurium DT009 isolate. Chemical mutagenesis caused attenuation via adenine and histidine auxotrophy, but did not change the phage type of the vaccine strain (Frech et al., 1998).
f. Chicken Response
  • Vaccination Protocol: One-day-old SPF chickens were inoculated orally with 0.1 ml of a Zoosaloral culture such that the birds received between 6 x 10^8 and 1 x 10^9 cfu each (Methner et al., 1997).
  • Persistence: Not noted.
  • Challenge Protocol: The birds were challenged 24 h later orally with 0.1 ml of a culture of nalidixic acid-resistant Salmonella derivative diluted in PBS such that each bird received 10^4 cfu(Methner et al., 1997).
  • Efficacy: A significant amount of S. Typhimurium 421 was fund in the livers of the chickens after pretreatment and challenge (Methner et al., 1997).
V. References
1. Adriaensen et al., 2007: Adriaensen C, De Greve H, Tian JQ, De Craeye S, Gubbels E, Eeckhaut V, Van Immerseel F, Ducatelle R, Kumar M, Hernalsteens JP. A live Salmonella enterica serovar Enteritidis vaccine allows serological differentiation between vaccinated and infected animals. Infection and immunity. 2007; 75(5); 2461-2468. [PubMed: 17261603].
2. Allam et al., 2011: Allam US, Krishna MG, Lahiri A, Joy O, Chakravortty D. Salmonella enterica Serovar Typhimurium Lacking hfq Gene Confers Protective Immunity against Murine Typhoid. PloS one. 2011; 6(2); e16667. [PubMed: 21347426].
3. Babu et al., 2004: Babu U, Dalloul RA, Okamura M, Lillehoj HS, Xie H, Raybourne RB, Gaines D, Heckert RA. Salmonella enteritidis clearance and immune responses in chickens following Salmonella vaccination and challenge. Veterinary immunology and immunopathology. 2004; 101(3-4); 251-257. [PubMed: 15350755].
4. Bansal et al., 2010: Bansal A, Paliwal PK, Sagi SS, Sairam M. Effect of adjuvants on immune response and protective immunity elicited by recombinant Hsp60 (GroEL) of Salmonella typhi against S. typhi infection. Molecular and cellular biochemistry. 2010; 337(1-2); 213-221. [PubMed: 19851830].
5. Boyle et al., 2007: Boyle EC, Bishop JL, Grassl GA, Finlay BB. Salmonella: from pathogenesis to therapeutics. Journal of bacteriology. 2007; 189(5); 1489-1495. [PubMed: 17189373].
6. Burns et al: Megan Egg – protection for commercial layers against Salmonella enteritidis infection for consumer protection [http://www.fda.gov/ohrms/dockets/dockets/00n0504/00N-0504_emc-001650-02.pdf]
7. Cao et al., 1992: Cao Y, Wen Z, Lu D. Construction of a recombinant oral vaccine against Salmonella typhi and Salmonella typhimurium. Infection and immunity. 1992; 60(7); 2823-2827. [PubMed: 1612747].
8. Cárdenas and Clements, 1992: Cárdenas L, Clements JD. Oral immunization using live attenuated Salmonella spp. as carriers of foreign antigens. Clinical microbiology reviews. 1992; 5(3); 328-342. [PubMed: 1498769].
9. Chatfield et al., 1992: Chatfield SN, Strahan K, Pickard D, Charles IG, Hormaeche CE, Dougan G. Evaluation of Salmonella typhimurium strains harbouring defined mutations in htrA and aroA in the murine salmonellosis model. Microbial pathogenesis. 1992; 12(2); 145-151. [PubMed: 1584006].
10. Chaudhuri et al., 2009: Chaudhuri RR, Peters SE, Pleasance SJ, Northen H, Willers C, Paterson GK, Cone DB, Allen AG, Owen PJ, Shalom G, Stekel DJ, Charles IG, Maskell DJ. Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice. PLoS pathogens. 2009; 5(7); e1000529. [PubMed: 19649318].
11. Cheminay and Hensel, 2007: Cheminay C, Hensel M. Rational design of Salmonella recombinant vaccines. International journal of medical microbiology : IJMM. 2007; ; . [PubMed: 17888730 ].
12. Choi et al., 2010: Choi J, Shin D, Ryu S. Salmonella enterica serovar Typhimurium ruvB mutant can confer protection against salmonellosis in mice. Vaccine. 2010; 28(39); 6436-6444. [PubMed: 20670908].
13. Coynault et al., 1996: Coynault C, Robbe-Saule V, Norel F. Virulence and vaccine potential of Salmonella typhimurium mutants deficient in the expression of the RpoS (sigma S) regulon. Molecular microbiology. 1996; 22(1); 149-160. [PubMed: 8899717].
14. Curtiss and Kelly, 1987: Curtiss R 3rd, Kelly SM. Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic. Infection and immunity. 1987; 55(12); 3035-3043. [PubMed: 3316029].
15. Curtiss et al., 1988: Curtiss R 3rd, Goldschmidt RM, Fletchall NB, Kelly SM. Avirulent Salmonella typhimurium delta cya delta crp oral vaccine strains expressing a streptococcal colonization and virulence antigen. Vaccine. 1988; 6(2); 155-160. [PubMed: 3291452].
16. Dorman et al., 1989: Dorman CJ, Chatfield S, Higgins CF, Hayward C, Dougan G. Characterization of porin and ompR mutants of a virulent strain of Salmonella typhimurium: ompR mutants are attenuated in vivo. Infection and immunity. 1989; 57(7); 2136-2140. [PubMed: 2543631].
17. Dougan et al., 1987: Dougan G, Maskell D, Pickard D, Hormaeche C. Isolation of stable aroA mutants of Salmonella typhi Ty2: properties and preliminary characterisation in mice. Molecular & general genetics : MGG. 1987; 207(2-3); 402-405. [PubMed: 3039297].
18. FDA: Vivotif: FDA: Vivotif Vaccine for Salmonella [http://www.fda.gov/BiologicsBloodVaccines/Vaccines/ApprovedProducts/ucm094070.htm]
19. Frech et al., 1998: Frech G, Weide-Botjes M, Nussbeck E, Rabsch W, Schwarz S. Molecular characterization of Salmonella enterica subsp. enterica serovar Typhimurium DT009 isolates: differentiation of the live vaccine strain Zoosaloral from field isolates. FEMS microbiology letters. 1998; 167(2); 263-269. [PubMed: 9809427].
20. Galdiero et al., 1999: Galdiero M, Marcatili A, Cipollaro de l'Ero G, Nuzzo I, Bentivoglio C, Galdiero M, Romano Carratelli C. Effect of transforming growth factor beta on experimental Salmonella typhimurium infection in mice. Infection and immunity. 1999; 67(3); 1432-1438. [PubMed: 10024591].
21. Game Bird Health - Broilact: Game Bird Health - Broilact [http://www.gamebirdhealth.co.uk/broilact.htm]
22. Germanier and Füer, 1975: Germanier R, Füer E. Isolation and characterization of Gal E mutant Ty 21a of Salmonella typhi: a candidate strain for a live, oral typhoid vaccine. The Journal of infectious diseases. 1975; 131(5); 553-558. [PubMed: 1092768].
23. Gilbreath et al., 2011: Gilbreath JJ, Colvocoresses Dodds J, Rick PD, Soloski MJ, Merrell DS, Metcalf ES. Enterobacterial Common Antigen Mutants of Salmonella enterica serovar Typhimurium Establish a Persistent Infection and Provide Protection Against Subsequent Lethal Challenge. Infection and immunity. 2011; ; . [PubMed: 22025511].
24. Hormaeche et al., 1991: Hormaeche CE, Joysey HS, Desilva L, Izhar M, Stocker BA. Immunity conferred by Aro- Salmonella live vaccines. Microbial pathogenesis. 1991; 10(2); 149-158. [PubMed: 1890952].
25. Humphreys et al., 1999: Humphreys S, Stevenson A, Bacon A, Weinhardt AB, Roberts M. The alternative sigma factor, sigmaE, is critically important for the virulence of Salmonella typhimurium. Infection and immunity. 1999; 67(4); 1560-1568. [PubMed: 10084987].
26. Kaneshige et al., 2009: Kaneshige T, Yaguchi K, Ohgitani T. Siderophore receptor IroN is an important protective antigen against Salmonella infection in chickens. Avian diseases. 2009; 53(4); 563-567. [PubMed: 20095157].
27. Kaniga et al., 1998: Kaniga K, Compton MS, Curtiss R 3rd, Sundaram P. Molecular and functional characterization of Salmonella enterica serovar typhimurium poxA gene: effect on attenuation of virulence and protection. Infection and immunity. 1998; 66(12); 5599-5606. [PubMed: 9826331].
28. Kincy-Cain et al., 1996: Kincy-Cain T, Clements JD, Bost KL. Endogenous and exogenous interleukin-12 augment the protective immune response in mice orally challenged with Salmonella dublin. Infection and immunity. 1996; 64(4); 1437-1440. [PubMed: 8606114].
29. Kustanova et al., 2006: Kustanova GA, Murashev AN, Karpov VL, Margulis BA, Guzhova IV, Prokhorenko IR, Grachev SV, Evgen'ev MB. Exogenous heat shock protein 70 mediates sepsis manifestations and decreases the mortality rate in rats. Cell stress & chaperones. 2006; 11(3); 276-286. [PubMed: 17009601].
30. Lee et al., 2007: Lee HY, Cho SA, Lee IS, Park JH, Seok SH, Baek MW, Kim DJ, Lee SH, Hur SJ, Ban SJ, Lee YK, Han YK, Cho YK, Park JH. Evaluation of phoP and rpoS mutants of Salmonella enterica serovar Typhi as attenuated typhoid vaccine candidates: virulence and protective immune responses in intranasally immunized mice. FEMS immunology and medical microbiology. 2007; 51(2); 310-318. [PubMed: 17725620].
31. Levine et al., 2007: Levine MM, Ferreccio C, Black RE, Lagos R, San Martin O, Blackwelder WC. Ty21a live oral typhoid vaccine and prevention of paratyphoid fever caused by Salmonella enterica Serovar Paratyphi B. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2007; 45 Suppl 1; S24-28. [PubMed: 17582564].
32. Liu et al., 2008: Liu T, König R, Sha J, Agar SL, Tseng CT, Klimpel GR, Chopra AK. Immunological responses against Salmonella enterica serovar Typhimurium Braun lipoprotein and lipid A mutant strains in Swiss-Webster mice: potential use as live-attenuated vaccines. Microbial pathogenesis. 2008; 44(3); 224-237. [PubMed: 17997275].
33. Matsuda et al., 2010: Matsuda K, Chaudhari AA, Kim SW, Lee KM, Lee JH. Physiology, pathogenicity and immunogenicity of lon and/or cpxR deleted mutants of Salmonella Gallinarum as vaccine candidates for fowl typhoid. Veterinary research. 2010; 41(5); 59. [PubMed: 20487719].
34. Matsui et al., 2003: Matsui H, Suzuki M, Isshiki Y, Kodama C, Eguchi M, Kikuchi Y, Motokawa K, Takaya A, Tomoyasu T, Yamamoto T. Oral immunization with ATP-dependent protease-deficient mutants protects mice against subsequent oral challenge with virulent Salmonella enterica serovar typhimurium. Infection and immunity. 2003; 71(1); 30-39. [PubMed: 12496146].
35. Methner et al., 1997: Methner U, Barrow PA, Martin G, Meyer H. Comparative study of the protective effect against Salmonella colonisation in newly hatched SPF chickens using live, attenuated Salmonella vaccine strains, wild-type Salmonella strains or a competitive exclusion product. International journal of food microbiology. 1997; 35(3); 223-230. [PubMed: 9105931].
36. Nagarajan et al., 2009: Nagarajan AG, Balasundaram SV, Janice J, Karnam G, Eswarappa SM, Chakravortty D. SopB of Salmonella enterica serovar Typhimurium is a potential DNA vaccine candidate in conjugation with live attenuated bacteria. Vaccine. 2009; 27(21); 2804-2811. [PubMed: 19428891].
37. Park et al., 2010: Park SI, Jeong JH, Choy HE, Rhee JH, Na HS, Lee TH, Her M, Cho KO, Hong Y. Immune response induced by ppGpp-defective Salmonella enterica serovar Gallinarum in chickens. Journal of microbiology (Seoul, Korea). 2010; 48(5); 674-681. [PubMed: 21046347].
38. Pasetti et al., 1999: Pasetti MF, Anderson RJ, Noriega FR, Levine MM, Sztein MB. Attenuated deltaguaBA Salmonella typhi vaccine strain CVD 915 as a live vector utilizing prokaryotic or eukaryotic expression systems to deliver foreign antigens and elicit immune responses. Clinical immunology (Orlando, Fla.). 1999; 92(1); 76-89. [PubMed: 10413655].
39. Paterson et al., 2009: Paterson GK, Northen H, Cone DB, Willers C, Peters SE, Maskell DJ. Deletion of tolA in Salmonella Typhimurium generates an attenuated strain with vaccine potential. Microbiology (Reading, England). 2009; 155(Pt 1); 220-228. [PubMed: 19118362].
40. Penha et al., 2010: Penha Filho RA, de Paiva JB, da Silva MD, de Almeida AM, Berchieri A Jr. Control of Salmonella Enteritidis and Salmonella Gallinarum in birds by using live vaccine candidate containing attenuated Salmonella Gallinarum mutant strain. Vaccine. 2010; 28(16); 2853-2859. [PubMed: 20153354].
41. Pesciaroli et al., 2011: Pesciaroli M, Aloisio F, Ammendola S, Pistoia C, Petrucci P, Tarantino M, Francia M, Battistoni A, Pasquali P. An attenuated Salmonella enterica serovar Typhimurium strain lacking the ZnuABC transporter induces protection in a mouse intestinal model of Salmonella infection. Vaccine. 2011; ; . [PubMed: 21219981].
42. Peters et al., 2010: Peters SE, Paterson GK, Bandularatne ES, Northen HC, Pleasance S, Willers C, Wang J, Foote AK, Constantino-Casas F, Scase TJ, Blacklaws BA, Bryant CE, Mastroeni P, Charles IG, Maskell DJ. Salmonella enterica serovar typhimurium trxA mutants are protective against virulent challenge and induce less inflammation than the live-attenuated vaccine strain SL3261. Infection and immunity. 2010; 78(1); 326-336. [PubMed: 19884329].
43. Piao et al., 2010: Piao HH, Tam VT, Na HS, Kim HJ, Ryu PY, Kim SY, Rhee JH, Choy HE, Kim SW, Hong Y. Immunological responses induced by asd and wzy/asd mutant strains of Salmonella enterica serovar Typhimurium in BALB/c mice. Journal of microbiology (Seoul, Korea). 2010; 48(4); 486-495. [PubMed: 20799091].
44. Roesler et al., 2004: Roesler U, Marg H, Schröder I, Mauer S, Arnold T, Lehmann J, Truyen U, Hensel A. Oral vaccination of pigs with an invasive gyrA-cpxA-rpoB Salmonella Typhimurium mutant. Vaccine. 2004; 23(5); 595-603. [PubMed: 15542179].
45. Rosu et al., 2007: Rosu V, Chadfield MS, Santona A, Christensen JP, Thomsen LE, Rubino S, Olsen JE. Effects of crp deletion in Salmonella enterica serotype Gallinarum. Acta veterinaria Scandinavica. 2007; 49; 14. [PubMed: 17488512].
46. Sagi et al., 2006: Sagi SS, Paliwal P, Bansal A, Mishra C, Khan N, Mustoori SR, Ilavazhagan G, Sawhney RC, Banerjee PK. Studies on immunogenicity and protective efficacy of DnaJ of Salmonella Typhi against lethal infection by Salmonella Typhimurium in mice. Vaccine. 2006; 24(49-50); 7135-7141. [PubMed: 16887241].
47. Salvat et al., 1992: Salvat G, Lalande F, Humbert F, Lahellec C. Use of a competitive exclusion product (Broilact) to prevent Salmonella colonization of newly hatched chicks. International journal of food microbiology. 1992; 15(3-4); 307-311. [PubMed: 1419536 ].
48. Schneitz and Renney, 2003: Schneitz C, Renney DJ. Effect of a commerical competitive exclusion product on the colonization of Salmonella infantis in day-old pheasant chicks. Avian diseases. 2003; 47(4); 1448-1451. [PubMed: 14708995 ].
49. Selke et al., 2007: Selke M, Meens J, Springer S, Frank R, Gerlach GF. Immunization of pigs to prevent disease in humans: construction and protective efficacy of a Salmonella enterica serovar Typhimurium live negative-marker vaccine. Infection and immunity. 2007; 75(5); 2476-2483. [PubMed: 17296750].
50. Sydenham et al., 2000: Sydenham M, Douce G, Bowe F, Ahmed S, Chatfield S, Dougan G. Salmonella enterica serovar typhimurium surA mutants are attenuated and effective live oral vaccines. Infection and immunity. 2000; 68(3); 1109-1115. [PubMed: 10678914].
51. Tacket and Levine, 2007: Tacket CO, Levine MM. CVD 908, CVD 908-htrA, and CVD 909 live oral typhoid vaccines: a logical progression. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2007; 45 Suppl 1; S20-23. [PubMed: 17582563].
52. Tacket et al., 2000: Tacket CO, Sztein MB, Wasserman SS, Losonsky G, Kotloff KL, Wyant TL, Nataro JP, Edelman R, Perry J, Bedford P, Brown D, Chatfield S, Dougan G, Levine MM. Phase 2 clinical trial of attenuated Salmonella enterica serovar typhi oral live vector vaccine CVD 908-htrA in U.S. volunteers. Infection and immunity. 2000; 68(3); 1196-1201. [PubMed: 10678926].
53. Tennant et al., 2011: Tennant SM, Wang JY, Galen JE, Simon R, Pasetti MF, Gat O, Levine MM. Engineering and pre-clinical evaluation of attenuated non-typhoidal Salmonella strains serving as live oral vaccines and as reagent strains. Infection and immunity. 2011; ; . [PubMed: 21807911].
54. Typhim Vi: FDA: Typhim Vi Vaccine for Salmonella [http://www.fda.gov/cber/label/typhimviLB.pdf]
55. Vorob'ev et al., 2009: Vorob'ev DS, Semenova IB, Kurbatova EA, Sveshnikov PG, Naroditskiĭ BS, Logunov DIu, Vaneeva NP. [Assessment of protective effect of heat shock protein-lypopolysaccharide of Salmonella typhimurium recombinant construction in mice]. Zhurnal mikrobiologii, epidemiologii, i immunobiologii. 2009; (3); 38-41. [PubMed: 19621817].
56. Wang et al., 2000: Wang JY, Noriega FR, Galen JE, Barry E, Levine MM. Constitutive expression of the Vi polysaccharide capsular antigen in attenuated Salmonella enterica serovar typhi oral vaccine strain CVD 909. Infection and immunity. 2000; 68(8); 4647-4652. [PubMed: 10899868].
57. Zhang-Barber et al., 1998: Zhang-Barber L, Turner AK, Dougan G, Barrow PA. Protection of chickens against experimental fowl typhoid using a nuoG mutant of Salmonella serotype Gallinarum. Vaccine. 1998; 16(9-10); 899-903. [PubMed: 9682335].