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Sarcocystis neurona

Table of Contents
  1. General Information
    1. NCBI Taxonomy ID
    2. Disease
    3. Introduction
    4. Microbial Pathogenesis
    5. Host Ranges and Animal Models
    6. Host Protective Immunity
  2. Vaccine Related Pathogen Genes
    1. SAG1 (Protective antigen)
    2. SAG1 (Protective antigen)
    3. SAG5 (Protective antigen)
  3. Vaccine Information
    1. Epm vaccine
    2. S. neurona SAG1 Protein Vaccine
  4. References
I. General Information
1. NCBI Taxonomy ID:
42890
2. Disease:
Equine protozoal myeloencephalitis
3. Introduction
Sarcocystis neurona is the most common cause of equine protozoal myeloencephalitis (EPM) in horses in America. It is a single celled parasite belonging to the group called coccidia (Apicomplexa: Sarcocystidae) with opossums as the definitive hosts and a variety of mammals as aberrant or natural intermediate hosts . Only asexual stages have been identified in the aberrant intermediate hosts, and they are confined to the brain and spinal cord, and any part of the central nervous system (CNS) may be affected. In histologic sections of CNS, individual merozoites are about 3-5 um long and contain a single, centrally located vesicular nucleus. The sarcocysts are microscopic (~700 um long) with a 1-3 um thick sarcocyst wall. The bradyzoites are slender and tiny (~ 5 um long). Sarcocystis neurona sporocysts from opossum faeces are ~ 10 x 8 um in size (USDA Agricultural Research Service: Sarcocystis neurona).
4. Microbial Pathogenesis
The pathogenesis of EPM is not clear because the complete life cycle is unknown. Sarcocystis neurona can parasitize all regions of the CNS, from the anterior cerebrum to the end of the spinal cord. Sarcocystis neurona schizonts and merozoites are found in neurons, mononuclear cells, glial cells, and perhaps other neural cells (USDA Agricultural Research Service: Sarcocystis neurona).
5. Host Ranges and Animal Models
Opossums (Didelphis virginiana, D. albiventris) are its definitive (reservoir) hosts and excrete oocysts and sporocysts (environmentally resistant stage)in their feces. Raccoons, armadillos, sea otters, skunks, cats and possibly other mammals are intermediate hosts (USDA Agricultural Research Service: Sarcocystis neurona).
6. Host Protective Immunity
Cell mediated immunity is an important component of controlling this intracellular parasite (Marsh et al., 2004).
1. SAG1
  • Gene Name : SAG1
  • Sequence Strain (Species/Organism) : Sarcocystis neurona 3106
  • VO ID : VO_0011253
  • NCBI Protein GI : 295682690
  • Other Database IDs : CDD:252367
  • Taxonomy ID : 42890
  • Gene Strand (Orientation) : ?
  • Protein Name : surface antigen 1
  • Protein pI : 7.22
  • Protein Weight : 26504.924
  • Protein Length : 333
  • Protein Note : SRS domain; pfam04092
  • Protein Sequence : Show Sequence
    >ADG26774.1 surface antigen 1 [Sarcocystis neurona]
    MTRAVLLTFLTLCSARVSLVRAGAPPQATCANGETTVTKLGSSGALRIHCPNNFRLAPRAGNDAGQMQVY
    ATAVAENPVNIRDVLPGASYLSVQNVPTLTVPQLPAKATSVFFHCQQQPDNQCFIQVEVAPAPRLGPNTC
    AALQSTIAFEVQQANETAVFSCGEGLAVFPQGSKALDEACSKEQALPSGAALAPKDGGLHLGFPQLPQQA
    MKICYICTNGGVQAEAAQRCEVRISVAANPDGSVPGANGAASXGAAARSAXALGLALVAGAFLHFC
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : Horses were vaccinated with adjuvanted recombinant SnSAG1 (rSnSAG1)and 5 control (sham vaccinated) horses were vaccinated with adjuvant only. The effect of vaccination with rSnSAG1 on in vivo infection by S. neurona was evaluated by challenging all the horses with S. neurona merozoites. The study showed that vaccination with rSnSAG-1 produced antibodies in horses that neutralized merozoites when tested by in vitro culture and significantly reduced clinical signs demonstrated by in vivo challenge (Ellison and Witonsky, 2009).
  • Related Vaccine(s): S. neurona SAG1 Protein Vaccine
2. SAG1
  • Gene Name : SAG1
  • Sequence Strain (Species/Organism) : Sarcocystis neurona
  • NCBI Protein GI : AAO41732
  • Taxonomy ID : 42890
  • Protein Name : merozoite surface antigen SAG1
  • Protein pI : 7.56
  • Protein Weight : 26619.054
  • Protein Length : 346
  • Protein Note : corresponds to mRNA found in GenBank Accession Number AY245695
  • Protein Sequence : Show Sequence
    >AAO41732.1 merozoite surface antigen SAG1 [Sarcocystis neurona]
    MTRAVLLTFLTLCSARVSLVRAGAPPQATCANGETTVTKLGSSGALRIHCPNNFRLAPRAGNDAGQMQVY
    ATAVAENPVNIRDVLPGASYLSVQNVPTLTVPQLPAKATSVFFHCQQQPDNQCFIQVEVAPAPRLGPNTC
    AALQSTIAFEVQQANETAVFSCGEGLAVFPQGSKALDEACSKEQALPSGAALAPKDGGLHLGFPQLPQQA
    MKICYICTNGGVQAEAAQRCEVRISVAANPDGSVPGANGAASXGAAARSAXALGLALVAGAFLHFC
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Elsheikha and Mansfield, 2004)
3. SAG5
  • Gene Name : SAG5
  • Sequence Strain (Species/Organism) : Sarcocystis neurona
  • NCBI Protein GI : ABD47681
  • Other Database IDs : CDD:282012
  • Taxonomy ID : 42890
  • Protein Name : surface antigen 5
  • Protein pI : 5.78
  • Protein Weight : 26623.63
  • Protein Length : 336
  • Protein Note : SRS domain; pfam04092
  • Protein Sequence : Show Sequence
    >ABD47681.1 surface antigen 5 [Sarcocystis neurona]
    MTRAVLLTILTLCSARVSFVNAGGARQATCEEGQTTATKLENPGVLQLQCPARYQLNPAPAADANEGMQV
    FTTAAVGNAVALQGVLPGATYVRAPNGAGATLTIPQLPPKAASVFIQCQQQGQQGQCFIEVQVAGSPRLG
    PNTCAAVQSRIDFEIRAQNEAAVFSCGAGRAPFPQALDDACSKEQSLPSGVALAPKDAGSFQLGFPQLPQ
    NPLKICYICTEGGQRVDAADQRCEVHIAVAGAEAGGPTGPTGGASVGPAARSASALVLAVVAAGFFHFW
    
    
  • Molecule Role : Protective antigen
  • Molecule Role Annotation : (Crowdus et al., 2008)
III. Vaccine Information
1. Epm vaccine
a. Tradename:
Epm vaccine
b. Manufacturer:
Fort Dodge
c. Vaccine Ontology ID:
VO_0000835
d. Type:
Inactivated or "killed" vaccine
e. Status:
Licensed
f. Host Species for Licensed Use:
Horse
g. Immunization Route
Intramuscular injection (i.m.)
h. Description
In vitro-cultured merozoites, chemically inactivated(Marsh et al., 2004)
2. S. neurona SAG1 Protein Vaccine
a. Vaccine Ontology ID:
VO_0004042
b. Type:
Subunit vaccine
c. Status:
Research
d. Gene Engineering of SAG1
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click here.
e. Adjuvant: Polygen vaccine adjuvant
f. Immunization Route
Intramuscular injection (i.m.)
g. Horse Response
  • Vaccination Protocol: The horses were vaccinated on days 0 and 21 with 1 mL adjuvanted (Polygen; MVP Laboratories, Omaha, Nebraska, USA) rSnSAG1 (50 μg) or 1 mL adjuvant alone by IM injection in the left side of the neck (Ellison and Witonsky, 2009).
  • Challenge Protocol: Horses in all groups were challenged on study day 36 with S. neurona merozoites (Ellison and Witonsky, 2009).
  • Efficacy: Vaccination with rSnSAG-1 produced antibodies in horses that neutralized merozoites when tested by in vitro culture and significantly reduced clinical signs demonstrated by in vivo challenge (Ellison and Witonsky, 2009).
IV. References
1. Crowdus et al., 2008: Crowdus CA, Marsh AE, Saville WJ, Lindsay DS, Dubey JP, Granstrom DE, Howe DK. SnSAG5 is an alternative surface antigen of Sarcocystis neurona strains that is mutually exclusive to SnSAG1. Veterinary parasitology. 2008; 158(1-2); 36-43. [PubMed: 18829171].
2. Ellison and Witonsky, 2009: Ellison S, Witonsky S. Evidence that antibodies against recombinant SnSAG1 of Sarcocystis neurona merozoites are involved in infection and immunity in equine protozoal myeloencephalitis. Canadian journal of veterinary research = Revue canadienne de recherche veterinaire. 2009; 73(3); 176-183. [PubMed: 19794889].
3. Elsheikha and Mansfield, 2004: Elsheikha HM, Mansfield LS. Sarcocystis neurona major surface antigen gene 1 (SAG1) shows evidence of having evolved under positive selection pressure. Parasitology research. 2004; 94(6); 452-459. [PubMed: 15517384].
4. Marsh et al., 2004: Marsh AE, Lakritz J, Johnson PJ, Miller MA, Chiang YW, Chu HJ. Evaluation of immune responses in horses immunized using a killed Sarcocystis neurona vaccine. Veterinary therapeutics : research in applied veterinary medicine. 2004; 5(1); 34-42. [PubMed: 15150728].
5. USDA Agricultural Research Service: Sarcocystis neurona: EPM/Sarcocystis neuorna [http://www.ars.usda.gov/Main/docs.htm?docid=11028]