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Vaccine Detail

B. bovis GST-12D3
Vaccine Information
  • Vaccine Ontology ID: VO_0011375
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: B. bovis T2Bo 12D3
  • 12D3 gene engineering:
    • Type: Recombinant protein preparation
    • Description: The 12D3 protective antigen was cloned and expressed as a glutathione-S-transferase (GST) fusion protein (Wright et al., 1992).
    • Detailed Gene Information: Click Here.
  • Adjuvant: complete Freunds adjuvant
  • Vector: pGEX
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Cattle Response

  • Vaccination Protocol: 50 micrograms of the GST-12D3 fusion protein was mixed with FCA and used to vaccinate cattle (Wright et al., 1992).
  • Challenge Protocol: Challenged with virulent B.bovis (Wright et al., 1992).
  • Efficacy: Immunised animals had parasitaemias one order of magnitude lower than controls when challenged with a heterologous strain of B. bovis. In a series of experiments, 100% of controls were treated, whereas only 16% of the GST-12D3 vaccinated animals required treatment (Wright et al., 1992).
References
Wright et al., 1992: Wright IG, Casu R, Commins MA, Dalrymple BP, Gale KR, Goodger BV, Riddles PW, Waltisbuhl DJ, Abetz I, Berrie DA. The development of a recombinant Babesia vaccine. Veterinary parasitology. 1992; 44(1-2); 3-13. [PubMed: 1441189].