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Vaccine Comparison

T. annulata Subunit SPAG-1 Protein Vaccine T. annulata Tams1 Protein Vaccine
Vaccine Information Vaccine Information
  • Vaccine Ontology ID: VO_0011517
  • Type: Subunit vaccine
  • Status: Research
  • spag-1 gene engineering:
    • Type: Recombinant protein preparation
    • Detailed Gene Information: Click Here.
  • Adjuvant: saponin vaccine adjuvant
  • Immunization Route: Intramuscular injection (i.m.)
  • Vaccine Ontology ID: VO_0004181
  • Type: Subunit vaccine
  • Status: Research
  • TA17050 merozoite-piroplasm surface antigen Tams1 gene engineering:
    • Type: Recombinant protein preparation
    • Detailed Gene Information: Click Here.
  • Adjuvant: ISCOM
    • VO ID: VO_0000757
    • Description: Immunostimulating complexes (ISCOMs) (d'Oliveira et al., 1997).
  • Immunization Route: Intramuscular injection (i.m.)
Host Response Host Response

Cattle Response

  • Vaccination Protocol: Three immunizations were given at 28 day intervals. The vaccination trials took place at two sites, the Centre for Tropical Veterinary Medicine (CTVM) in Edinburgh and Utrecht University, the Netherlands. The four CTVM groups (groups I, II, III and IV) were immunized with His6-SPAG-1 in RWL, NS1-p67 in RWL, His6-SPAG-1 incorporated into ISCOMs and PBS-RWL control, respectively, at the doses indicated. RWL is a proprietary saponin-based adjuvant supplied by SmithKline Beecham. The NS1-p67 has been previously described 8 and was supplied by collaborators at ILRI, Nairobi, Kenya. The four groups of cattle at Utrecht University (groups V, VI, VII and VIII) were immunized with His6-SPAG-1, His6-TAMS 1-1/1-2, His6-SPAG-1 plus TAMS 1-1/1-2, all incorporated into ISCOMs and an ISCOM control, at the doses indicated (Boulter et al., 1998).
  • Challenge Protocol: Fourteen days after the third immunization all the calves were challenged with an ∼LD50 (0.2 tick equivalents) of the same T. annulata Hissar sporozoite stabilate.
  • Efficacy: All the data collected from these two trials indicate that SPAG-1 administered with RWL is better at inducing an effective immune response than SPAG-1 incorporated into ISCOMs, with or without the presence of merozoite antigens. The SPAG-1 RWL group appeared to be the best protected to challenge. The prepatent period in this group, i.e. the time to when the first macroschizont is seen in lymph node smears, is significantly increased compared to controls, as is the incubation period. Furthermore, 3 of the animals in this group survived challenge (Boulter et al., 1998).

Cattle Response

  • Vaccination Protocol: Calves were immunized via intramuscular injection with 1 ml ISCOM vaccine given intramuscularly, containing 20 μg re-(Tamsl-l or Tams1 -2) (d'Oliveira et al., 1997).
  • Challenge Protocol: Calves were challenged with T. annulata Ankara strain (d'Oliveira et al., 1997).
  • Efficacy: All calves vaccinated with ISCOMs proved to be protected from T. annulata infection and had generated antibodies against both re-(Tams1-1 and Tams1-2) at the time of challenge (d'Oliveira et al., 1997).
References References
Boulter et al., 1998: Boulter NR, Brown CG, Kirvar E, Glass E, Campbell J, Morzaria S, Nene V, Musoke A, D'Oliveira C, Gubbels MJ, Jongejan F, Hall FR. Different vaccine strategies used to protect against Theileria annulata. Annals of the New York Academy of Sciences. 1998; 849; 234-246. [PubMed: 9668470].
d'Oliveira et al., 1997: d'Oliveira C, Feenstra A, Vos H, Osterhaus AD, Shiels BR, Cornelissen AW, Jongejan F. Induction of protective immunity to Theileria annulata using two major merozoite surface antigens presented by different delivery systems. Vaccine. 1997; 15(16); 1796-1804. [PubMed: 9364686].