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Vaccine Detail

Phleum pratense Allergy Phl p 12 Subunit Vaccine
Vaccine Information
  • Vaccine Ontology ID: VO_0011374
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: A recombinant protein comprised of the C terminus of Phl p 12 at its N terminus and the Phl p 12 N terminus at its C terminus, Phl p 12-rs, was expressed in Escherichia coli and purified (Westritschnig et al., 2007).
  • PRO2 gene engineering:
    • Type: Recombinant protein preparation
    • Detailed Gene Information: Click Here.
  • Adjuvant: aluminum hydroxide vaccine adjuvant
  • Immunization Route: subcutaneous injection
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunized s.c. with either 10 µg of rPhl p 12, 10 µg of rPhl p 12-rs, or a equimolar mixture of the five peptides (2 µg of each peptide) adsorbed to aluminum hydroxide (Westritschnig et al., 2007).
  • Immune Response: rPhl p 12-rs induced IgG1 reactivity to rPhl p 12, rBet v 2, rCor a 2, and rDau c 4 that was of comparable magnitude as the reactivity induced by rPhl p 12 (Westritschnig et al., 2007).
  • Efficacy: rPhl p 12-rs and the Phl p 12-derived peptides induced lower allergenic immune responses to the wild-type allergen than rPhl p 12 upon immunization of the mice and thus exhibited reduced in vivo allergenicity (Westritschnig et al., 2007).
References
Westritschnig et al., 2007: Westritschnig K, Linhart B, Focke-Tejkl M, Pavkov T, Keller W, Ball T, Mari A, Hartl A, Stöcklinger A, Scheiblhofer S, Thalhamer J, Ferreira F, Vieths S, Vogel L, Böhm A, Valent P, Valenta R. A hypoallergenic vaccine obtained by tail-to-head restructuring of timothy grass pollen profilin, Phl p 12, for the treatment of cross-sensitization to profilin. Journal of immunology (Baltimore, Md. : 1950). 2007; 179(11); 7624-7634. [PubMed: 18025208].