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S. mansoni DNA vaccine pcDNA/CTSOD encoding SOD |
S. mansoni DNA vaccine pcDNA/GPXbb encoding glutathione-S-peroxidase, GPX |
S. mansoni DNA vaccine Sm-p80-pcDNA3 encoding the large subunit of S. mansoni calpain |
S. mansoni DNA vaccine Sm23-pcDNA encoding Sm23 |
| Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
- Vaccine Ontology ID: VO_0004462
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- SOD
gene engineering:
- Type: DNA vaccine construction
- Description: Vector cDNAI/Amp (in vitrogen) expressed Cu/Zn superoxide dismutase, SOD (Shalaby et al., 2003).
- Detailed Gene Information: Click Here.
- Vector: pcDNAI/Amp (Shalaby et al., 2003)
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0004463
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- GPX1
gene engineering:
- Type: DNA vaccine construction
- Detailed Gene Information: Click Here.
- Vector: pcDNAI/Amp (in vitrogen) (Shalaby et al., 2003)
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0004464
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- calpain
gene engineering:
- Type: DNA vaccine construction
- Detailed Gene Information: Click Here.
- Vector: pcDNA3 (Siddiqui et al., 2003)
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0004461
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- Sm23
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pcDNA1.1/Amp expressed Schistosoma mansoni integral membrane protein Sm23 (Da'dara et al., 2001).
- Detailed Gene Information: Click Here.
- Vector: pcDNA1.1/Amp (Da'dara et al., 2001)
- Immunization Route: Intramuscular injection (i.m.)
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| Host Response |
Host Response |
Host Response |
Host Response |
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Mouse Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: CT-SOD showed the highest level of protection out of all vaccines tested (54%). Two immunizations resulted in significant reduction of worm burden compared to control groups (Shalaby et al., 2003).
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Mouse Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: For the regimens involving GPXbb, immunization with DNA vaccination alone resulted in protection of 44%, but the prime-boost (boost of recombinant vaccinia virus/GPXbb) resulted in 85% worm burden reduction (Shalaby et al., 2003).
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Mouse Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: Mice immunized with Sm-p80-pcDNA3 showed statistically significant 39% reduction in worm burden when compared with mice which received only pcDNA3. Similarly, mice immunized with Sm-p80-pcDNA3 and co-injected with pORF-mIL-2 exhibited 57% reduction (P≤0.0001) in the number of parasites when compared with the group of mice receiving only pcDNA3 and pORF-mIL-2. Also, when mice were primed with Sm-p80-pcDNA3 in the presence of pORF-mIL-12, they contained 45% less number of parasites (P≤0.0001) compared to the group of mice which were immunized with pcDNA3 alone (Siddiqui et al., 2003).
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Mouse Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: The Sm23-pcDNA vaccine provided statistically significant levels of protection against challenge infection (21-44%, P<0.001-0.02) (Da'dara et al., 2001).
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| References |
References |
References |
References |
Shalaby et al., 2003: Shalaby KA, Yin L, Thakur A, Christen L, Niles EG, LoVerde PT. Protection against Schistosoma mansoni utilizing DNA vaccination with genes encoding Cu/Zn cytosolic superoxide dismutase, signal peptide-containing superoxide dismutase and glutathione peroxidase enzymes. Vaccine. 2003; 22(1); 130-136. [PubMed: 14604580].
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Shalaby et al., 2003: Shalaby KA, Yin L, Thakur A, Christen L, Niles EG, LoVerde PT. Protection against Schistosoma mansoni utilizing DNA vaccination with genes encoding Cu/Zn cytosolic superoxide dismutase, signal peptide-containing superoxide dismutase and glutathione peroxidase enzymes. Vaccine. 2003; 22(1); 130-136. [PubMed: 14604580].
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Siddiqui et al., 2003: Siddiqui AA, Phillips T, Charest H, Podesta RB, Quinlin ML, Pinkston JR, Lloyd JD, Pompa J, Villalovos RM, Paz M. Enhancement of Sm-p80 (large subunit of calpain) induced protective immunity against Schistosoma mansoni through co-delivery of interleukin-2 and interleukin-12 in a DNA vaccine formulation. Vaccine. 2003; 21(21-22); 2882-2889. [PubMed: 12798631].
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Da'dara et al., 2001: Da'dara AA, Skelly PJ, Wang MM, Harn DA. Immunization with plasmid DNA encoding the integral membrane protein, Sm23, elicits a protective immune response against schistosome infection in mice. Vaccine. 2001; 20(3-4); 359-369. [PubMed: 11672898].
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