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Vaccine Comparison

Vaccinia virus DNA vaccine 4pox Vaccinia virus DNA vaccine A33R from strain IHD-J Vaccinia virus DNA vaccine B5R Vaccinia virus DNA vaccine pWRG/L1R Vaccinia virus DNA vaccine tPA-D8L Vaccinia virus DNA vaccine wtD8L
Vaccine Information Vaccine Information Vaccine Information Vaccine Information Vaccine Information Vaccine Information
  • Vaccine Ontology ID: VO_0004485
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • A27L gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pWRG/TPA expressed VACV A27L (Hooper et al., 2007).
    • Detailed Gene Information: Click Here.
  • L1R gene engineering:
    • Type: DNA vaccine construction
    • Description: This vaccine expressed L1R (Hooper et al., 2007).
    • Detailed Gene Information: Click Here.
  • A33R gene engineering:
    • Type: DNA vaccine construction
    • Description: This vaccine expressed A33R (Hooper et al., 2007).
    • Detailed Gene Information: Click Here.
  • B5R gene engineering:
    • Type: DNA vaccine construction
    • Description: This vaccine expressed B5R (Hooper et al., 2007).
    • Detailed Gene Information: Click Here.
  • Vector: pWRG/TPA vector (Hooper et al., 2007)
  • Immunization Route: Intramuscular injection (i.m.)
  • Vaccine Ontology ID: VO_0004482
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • A33R gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pCI (Promega) expressed the extraviral domains of A33R (Galmiche et al., 1999).
    • Detailed Gene Information: Click Here.
  • Vector: pCI (Promega) (Galmiche et al., 1999)
  • Immunization Route: Intramuscular injection (i.m.)
  • Vaccine Ontology ID: VO_0004483
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • B5R gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pCI (Promega) expressed the complete open reading frame of B5R (Galmiche et al., 1999).
    • Detailed Gene Information: Click Here.
  • Vector: pCI (Promega) (Galmiche et al., 1999)
  • Immunization Route: Intramuscular injection (i.m.)
  • Vaccine Ontology ID: VO_0004484
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • L1R gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pWRG7077 expressed L1R genes from VACV (Hooper et al., 2000).
    • Detailed Gene Information: Click Here.
  • Vector: pWRG7077 (Hooper et al., 2000)
  • Immunization Route: Gene gun
  • Vaccine Ontology ID: VO_0004487
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • D8L gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pVax1TM expressed the transmembrane (TM) region and its downstream cytoplasm tail were removed from the wild type D8L gene , and a human tissue plasminogen activator (tPA) leader sequence was added to the N terminus of D8L coding gene (Sakhatskyy et al., 2006).
    • Detailed Gene Information: Click Here.
  • Vector: pVax1TM (Sakhatskyy et al., 2006)
  • Immunization Route: Gene gun
  • Vaccine Ontology ID: VO_0004486
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • D8L gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pSW3891 expressed the full length coding sequence of the wild type D8L gene (wtD8L) (Sakhatskyy et al., 2006).
    • Detailed Gene Information: Click Here.
  • Vector: pSW3891 (Sakhatskyy et al., 2006)
  • Immunization Route: Gene gun
Host Response Host Response Host Response Host Response Host Response Host Response

Mouse Response

  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: Vaccinated mice were completely protected against a lethal (>10LD(50)) intranasal challenge with vaccinia virus strain IHD-J (Hooper et al., 2007).

Mouse Response

  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: Vaccination with A33R protein also provided protection, resulting in a 100% survival rate and in a significantly lower body weight loss and a more rapid recovery from infection than with B5R protein (Galmiche et al., 1999).

Mouse Response

  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: After challenge with lethal doses of vaccinia virus, 90% of the mice vaccinated with B5R protein were protected (Galmiche et al., 1999).

Mouse Response

  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: Mice vaccinated with L1R alone developed neutralizing antibodies and were partially protected (Hooper et al., 2000).

Mouse Response

  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: Individual outer membrane proteins from intracellular and extracellular virions of vaccinia virus, when delivered in the form of either DNA vaccines or recombinant protein vaccines produced from baculovirus-infected insect cells, were able to protect mice from the vaccinia virus challenge and rhesus macaques from the monkeypox virus challenge (Sakhatskyy et al., 2006).

Mouse Response

  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: Individual outer membrane proteins from intracellular and extracellular virions of vaccinia virus, when delivered in the form of either DNA vaccines or recombinant protein vaccines produced from baculovirus-infected insect cells, were able to protect mice from the vaccinia virus challenge and rhesus macaques from the monkeypox virus challenge (Sakhatskyy et al., 2006).
References References References References References References
Hooper et al., 2007: Hooper JW, Golden JW, Ferro AM, King AD. Smallpox DNA vaccine delivered by novel skin electroporation device protects mice against intranasal poxvirus challenge. Vaccine. 2007; 25(10); 1814-1823. [PubMed: 17240007].
Galmiche et al., 1999: Galmiche MC, Goenaga J, Wittek R, Rindisbacher L. Neutralizing and protective antibodies directed against vaccinia virus envelope antigens. Virology. 1999; 254(1); 71-80. [PubMed: 9927575].
Galmiche et al., 1999: Galmiche MC, Goenaga J, Wittek R, Rindisbacher L. Neutralizing and protective antibodies directed against vaccinia virus envelope antigens. Virology. 1999; 254(1); 71-80. [PubMed: 9927575].
Hooper et al., 2000: Hooper JW, Custer DM, Schmaljohn CS, Schmaljohn AL. DNA vaccination with vaccinia virus L1R and A33R genes protects mice against a lethal poxvirus challenge. Virology. 2000; 266(2); 329-339. [PubMed: 10639319].
Sakhatskyy et al., 2006: Sakhatskyy P, Wang S, Chou TH, Lu S. Immunogenicity and protection efficacy of monovalent and polyvalent poxvirus vaccines that include the D8 antigen. Virology. 2006; 355(2); 164-174. [PubMed: 16919703].
Sakhatskyy et al., 2006: Sakhatskyy P, Wang S, Chou TH, Lu S. Immunogenicity and protection efficacy of monovalent and polyvalent poxvirus vaccines that include the D8 antigen. Virology. 2006; 355(2); 164-174. [PubMed: 16919703].