VIOLIN Logo
VO Banner
Search: for Help
About
Introduction
Statistics
VIOLIN News
Your VIOLIN
Register or Login
Submission
Tutorial
Vaccine & Components
Vaxquery
Vaxgen
VBLAST
Protegen
VirmugenDB
DNAVaxDB
CanVaxKB
Vaxjo
Vaxvec
Vevax
Huvax
Vaccine Mechanisms
Vaximmutordb
Vaxism
Vaxar
Vaccine Literature
VO-SciMiner
Litesearch
Vaxmesh
Vaxlert
Vaccine Design
Vaxign
Community Efforts
Vaccine Ontology
ICoVax 2012
ICoVax 2013
Advisory Committee
Vaccine Society
Vaxperts
VaxPub
VaxCom
VaxLaw
VaxMedia
VaxMeet
VaxFund
VaxCareer
Data Exchange
V-Utilities
VIOLINML
Help & Documents
Publications
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

Vaccine Comparison

HRSV DNA vaccine DRF-412 HRSV DNA vaccine pND-G
Vaccine Information Vaccine Information
  • Vaccine Ontology ID: VO_0004579
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • F gene engineering:
    • Type: DNA vaccine construction
    • Detailed Gene Information: Click Here.
  • Vector: phCMV1 (Wu et al., 2009)
  • Immunization Route: Intramuscular injection (i.m.)
  • Vaccine Ontology ID: VO_0004580
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • G gene engineering:
    • Type: DNA vaccine construction
    • Detailed Gene Information: Click Here.
  • Vector: pND (Miller et al., 2002)
  • Immunization Route: Intradermal injection (i.d.)
Host Response Host Response

Mouse Response

  • Vaccine Immune Response Type: VO_0003057
  • Immune Response: The DRF-412 vaccine vector was as effective as live RSV in inducing neutralization antibody, systemic Ab (IgG, IgG1, IgG2a, and IgG2b) responses, and mucosal antibody responses (Ig A). Mice inoculated with vector DRF-412 induced a higher mixed Th1/Th2 cytokine immune response than DRF-412-P (Wu et al., 2009).
  • Efficacy: Mice immunized with DRF-412 and DRF-412-P have a higher average Ct value compared with PBS and phCMV I group (P < 0.001), which means that they have less viral mRNA in the lung indicating better protection, but there was no statistically significant difference between DRF-412 and DRF-412-P. This vaccine induced partial protection against RSV in mice (Wu et al., 2009).

Mouse Response

  • Vaccine Immune Response Type: VO_0003057
  • Efficacy: Immunization with the pND-G vaccine significantly inhibited the RSV induced increase in airway responsiveness to methacholine (Mch) (n=3 separate experiments; 12 mice per group) (P<0.05 versus RSV). The concentration of Mch required to induce a 200% increase in airway responsiveness (PC200) was significantly greater in RSV infected mice who had received the pND-G vaccine compared to RSV infected mice who had not received the vaccine (21.5 mg/ml Mch versus 7.2 mg/ml Mch) (P<0.05) (Miller et al., 2002).
References References
Wu et al., 2009: Wu H, Dennis VA, Pillai SR, Singh SR. RSV fusion (F) protein DNA vaccine provides partial protection against viral infection. Virus research. 2009; 145(1); 39-47. [PubMed: 19540885].
Miller et al., 2002: Miller M, Cho JY, Baek KJ, Castaneda D, Nayar J, Rodriguez M, Roman M, Raz E, Broide DH. Plasmid DNA encoding the respiratory syncytial virus G protein protects against RSV-induced airway hyperresponsiveness. Vaccine. 2002; 20(23-24); 3023-3033. [PubMed: 12126916].