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Vaccine Detail

V. cholerae CTB DNA Vaccine
Vaccine Information
  • Vaccine Ontology ID: VO_0011360
  • Type: DNA vaccine
  • Status: Research
  • Antigen: cholera toxin B subunit (CTB) (Xu et al., 2009).
  • ctxB gene engineering:
    • Type: DNA vaccine construction
    • Detailed Gene Information: Click Here.
  • Vector: pJW4303 (Xu et al., 2009)
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Host Strain: BALB/c mice
  • Vaccination Protocol: Mice were immunized intramuscularly with 100 μg of the CTB DNA vaccine or vector control. The DNA immunizations for mice were given at Weeks 0 and 2 in DNA alone prime boost groups, or Week 0 only for those receiving DNA prime followed by KWC-B vaccine boost at Week 2 (Xu et al., 2009).
  • Challenge Protocol: Naïve suckling BALB/C mice at 5- to 6-day-old were separated from their mothers for 4 h, then inoculated by oral gavage with 50 μl of the appropriate serial dilution of the inoculum. To prepare the inoculum, V. cholerae O1 El Tor biotype Ogawa strain V48-65 growing overnight on an LB plate, were re-suspended in PBS and adjusted to 5 LD50 in 25 μl, then incubated with 25 of properly diluted immune or normal rabbit serum samples for 1 h at 37 °C. The suckling mice were kept in a 25 °C environment and observed every 4 h for 48 h post-inoculation. The survival rates were documented at each time point (Xu et al., 2009).
  • Efficacy: The immune sera elicited by CTB DNA vaccine were functional and protective against V. cholerae O1 infection in mice (Xu et al., 2009).
References
Xu et al., 2009: Xu G, Wang S, Zhuang L, Hackett A, Gu L, Zhang L, Zhang C, Wang H, Huang Z, Lu S. Intramuscular delivery of a cholera DNA vaccine primes both systemic and mucosal protective antibody responses against cholera. Vaccine. 2009; 27(29); 3821-3830. [PubMed: 19443090].