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Vaccine Comparison

TBEV DNA vaccine encoding E protein Tick-borne Encephalitis Virus C protein mutant vaccine
Vaccine Information Vaccine Information
  • Vaccine Ontology ID: VO_0004154
  • Type: DNA vaccine
  • Status: Research
  • TBEVgp1 polyprotein gene engineering:
    • Type: DNA vaccine construction
    • Detailed Gene Information: Click Here.
  • Vector: pCMV-β (Aberle et al., 1999)
  • Immunization Route: Intramuscular injection (i.m.)
  • Vaccine Ontology ID: VO_0004278
  • Type: Live, attenuated vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • C protein gene engineering:
    • Type: Gene mutation
    • Description: This C protein mutant is from Tick-borne encephalitis virus (Kofler et al., 2002).
    • Detailed Gene Information: Click Here.
  • Immunization Route: subcutaneous injection
Host Response Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Groups of 16 BALB/c mice each received primary and booster immunizations spaced 4 wk apart. Plasmids were purified and administered i.m. (100 µg/dose) or by particle bombardment with DNA-coated gold beads (2 µg/dose) using the helium-powered Helios Gene Gun delivery system. Intramuscular inoculation of DNA in saline was done as a 100-µl injection in the right quadriceps. For Gene Gun inoculation, 1.0 µg of the same DNA was coupled to 0.5 mg of 1.0-µm-diameter gold particles, as recommended by the manufacturer (Bio-Rad). DNA-coated microcarriers were delivered into the abdominal epidermis of mice using the Gene Gun at a helium pressure setting of 400 psi. Mice immunized with the commercial TBE vaccine received 0.2 ml s.c. inoculations containing 1 µg formalin-inactivated virus (FSME Immun Inject, Baxter-Immuno, Vienna, Austria) (Aberle et al., 1999).
  • Challenge Protocol: Mice were subsequently challenged by i.p. inoculation with 1000 LD50 of the highly mouse-pathogenic TBE virus strain Hypr. (Aberle et al., 1999).
  • Efficacy: The plasmid construct encoding a secreted subviral particle, which carries multiple copies of the protective Ag on its surface, was superior to the other constructs in terms of extent and functionality of the Ab response as well as protection against virus challenge (Aberle et al., 1999).

Mouse Response

  • Persistence: A C protein mutant is attenuated in mice (Kofler et al., 2002).
  • Efficacy: A C protein mutant induced protection in mice from challenge with wild type TBEV (Kofler et al., 2002).
References References
Aberle et al., 1999: Aberle JH, Aberle SW, Allison SL, Stiasny K, Ecker M, Mandl CW, Berger R, Heinz FX. A DNA immunization model study with constructs expressing the tick-borne encephalitis virus envelope protein E in different physical forms. Journal of immunology (Baltimore, Md. : 1950). 1999; 163(12); 6756-6761. [PubMed: 10586074].
Kofler et al., 2002: Kofler RM, Heinz FX, Mandl CW. Capsid protein C of tick-borne encephalitis virus tolerates large internal deletions and is a favorable target for attenuation of virulence. Journal of virology. 2002; 76(7); 3534-3543. [PubMed: 11884577].