• Vaccine Ontology ID: VO_0004409
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• Collagen type II gene engineering:
  • Type: DNA vaccine construction
  • Detailed Gene Information: Click Here.
• Immunization Route: Intramuscular injection (i.m.)

• Vaccine Ontology ID: VO_0004408
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• hsp65 gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3 expressed hsp65 from M. leprae (Santos-Junior et al., 2005).
  • Detailed Gene Information: Click Here.
• Vector: pcDNA3 (Santos-Junior et al., 2005)
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: In vitro analysis of anti-CII T cells demonstrated reduced production of the pro-inflammatory cytokines IFN-γ and TNF-α in CII vaccinated mice. Further, CII DNA vaccines reduced epitope spreading of auto-reactive B cell responses in CIA (Ho et al., 2006).
• Efficacy: DNA encoding CII reduced the disease severity and disease incidence of collagen-induced arthritis to 50% as compared to the PBS-treated control group in which the disease was more severe and the disease incidence was 89% (Ho et al., 2006).
• Host Ifng (Interferon gamma) response
  • Description: CII-stimulated lymphocytes isolated from CIA mice treated with a DNA vaccine encoding CII produced significantly less IFN-g and TNF-a compared to PBS and DNA vector-IL-4 treated mice (Ho et al., 2006).
  • Detailed Gene Information: Click Here.
• Host TNF-alpha response
  • Description: CII-stimulated lymphocytes isolated from CIA mice treated with a DNA vaccine encoding CII produced significantly less IFN-g and TNF-a compared to PBS and DNA vector-IL-4 treated mice (Ho et al., 2006).
  • Detailed Gene Information: Click Here.

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: Cellular and humoral immune responses to the protein are elicited, and long-lived memory lymphocytes are induced (Santos-Junior et al., 2005).
• Efficacy: DNA-hsp65 vaccination was protective against arthritis in AIRmax mice (maximal acute inflammatory reaction), as evidenced by the absence of arthritis incidence during the 150-day follow-up period. This protective effect was more pronounced after the onset of pristane-induced arthritis (Santos-Junior et al., 2005).