• Vaccine Ontology ID: VO_0004485
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• A27L gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pWRG/TPA expressed VACV A27L (Hooper et al., 2007).
  • Detailed Gene Information: Click Here.
• L1R gene engineering:
  • Type: DNA vaccine construction
  • Description: This vaccine expressed L1R (Hooper et al., 2007).
  • Detailed Gene Information: Click Here.
• A33R gene engineering:
  • Type: DNA vaccine construction
  • Description: This vaccine expressed A33R (Hooper et al., 2007).
  • Detailed Gene Information: Click Here.
• B5R gene engineering:
  • Type: DNA vaccine construction
  • Description: This vaccine expressed B5R (Hooper et al., 2007).
  • Detailed Gene Information: Click Here.
• Vector: pWRG/TPA vector (Hooper et al., 2007)
• Immunization Route: Intramuscular injection (i.m.)

• Vaccine Ontology ID: VO_0004482
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• A33R gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pCI (Promega) expressed the extraviral domains of A33R (Galmiche et al., 1999).
  • Detailed Gene Information: Click Here.
• Vector: pCI (Promega) (Galmiche et al., 1999)
• Immunization Route: Intramuscular injection (i.m.)

• Vaccine Ontology ID: VO_0004483
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• B5R gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pCI (Promega) expressed the complete open reading frame of B5R (Galmiche et al., 1999).
  • Detailed Gene Information: Click Here.
• Vector: pCI (Promega) (Galmiche et al., 1999)
• Immunization Route: Intramuscular injection (i.m.)

• Vaccine Ontology ID: VO_0004484
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• L1R gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pWRG7077 expressed L1R genes from VACV (Hooper et al., 2000).
  • Detailed Gene Information: Click Here.
• Vector: pWRG7077 (Hooper et al., 2000)
• Immunization Route: Gene gun

• Vaccine Ontology ID: VO_0004487
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• D8L gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pVax1TM expressed the transmembrane (TM) region and its downstream cytoplasm tail were removed from the wild type D8L gene , and a human tissue plasminogen activator (tPA) leader sequence was added to the N terminus of D8L coding gene (Sakhatskyy et al., 2006).
  • Detailed Gene Information: Click Here.
• Vector: pVax1TM (Sakhatskyy et al., 2006)
• Immunization Route: Gene gun

• Vaccine Ontology ID: VO_0004486
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• D8L gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pSW3891 expressed the full length coding sequence of the wild type D8L gene (wtD8L) (Sakhatskyy et al., 2006).
  • Detailed Gene Information: Click Here.
• Vector: pSW3891 (Sakhatskyy et al., 2006)
• Immunization Route: Gene gun

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: Vaccinated mice were completely protected against a lethal (>10LD(50)) intranasal challenge with vaccinia virus strain IHD-J (Hooper et al., 2007).

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: Vaccination with A33R protein also provided protection, resulting in a 100% survival rate and in a significantly lower body weight loss and a more rapid recovery from infection than with B5R protein (Galmiche et al., 1999).

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: After challenge with lethal doses of vaccinia virus, 90% of the mice vaccinated with B5R protein were protected (Galmiche et al., 1999).

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: Mice vaccinated with L1R alone developed neutralizing antibodies and were partially protected (Hooper et al., 2000).

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: Individual outer membrane proteins from intracellular and extracellular virions of vaccinia virus, when delivered in the form of either DNA vaccines or recombinant protein vaccines produced from baculovirus-infected insect cells, were able to protect mice from the vaccinia virus challenge and rhesus macaques from the monkeypox virus challenge (Sakhatskyy et al., 2006).

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: Individual outer membrane proteins from intracellular and extracellular virions of vaccinia virus, when delivered in the form of either DNA vaccines or recombinant protein vaccines produced from baculovirus-infected insect cells, were able to protect mice from the vaccinia virus challenge and rhesus macaques from the monkeypox virus challenge (Sakhatskyy et al., 2006).