• Vaccine Ontology ID: VO_0004539
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• spaA gene engineering:
  • Type: DNA vaccine construction
  • Description: This vaccine encoded a fusion of the spaA protein and various elements, such as a secretion leader sequence from the highly expressed human gene encoding alpha1-antitrypsin (AAT), a highly soluble and stably folded domain from the rat cartilage oligomerization matrix protein (COMP), and three copies of the complement component, C3d3 (Chen et al., 2009).
  • Detailed Gene Information: Click Here.
• Vector: pcDNA3 (Chen et al., 2009)
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: Immunization with the DNA vaccine expressing pcDNA3-AAT-COMP-spaAN-3C3d (pcD-ACSC) had higher antibody titers than pcDNA3-spaA(N) (pcD-S) at week 4 (Chen et al., 2009).
• Efficacy: The protective efficacy of the spaA-chimeras was demonstrated by lethal challenge with a virulent homologous strain 1249 against immunized mice (Chen et al., 2009).