• Vaccine Ontology ID: VO_0004579
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• F protein gene engineering:
  • Type: DNA vaccine construction
  • Detailed Gene Information: Click Here.
• Vector: phCMV1 (Wu et al., 2009)
• Immunization Route: Intramuscular injection (i.m.)

• Vaccine Ontology ID: VO_0004580
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• G gene engineering:
  • Type: DNA vaccine construction
  • Detailed Gene Information: Click Here.
• Vector: pND (Miller et al., 2002)
• Immunization Route: Intradermal injection (i.d.)

Mouse Response

• Vaccine Immune Response Type: VO_0003057
• Immune Response: The DRF-412 vaccine vector was as effective as live RSV in inducing neutralization antibody, systemic Ab (IgG, IgG1, IgG2a, and IgG2b) responses, and mucosal antibody responses (Ig A). Mice inoculated with vector DRF-412 induced a higher mixed Th1/Th2 cytokine immune response than DRF-412-P (Wu et al., 2009).
• Efficacy: Mice immunized with DRF-412 and DRF-412-P have a higher average Ct value compared with PBS and phCMV I group (P < 0.001), which means that they have less viral mRNA in the lung indicating better protection, but there was no statistically significant difference between DRF-412 and DRF-412-P. This vaccine induced partial protection against RSV in mice (Wu et al., 2009).

Mouse Response

• Vaccine Immune Response Type: VO_0003057
• Efficacy: Immunization with the pND-G vaccine significantly inhibited the RSV induced increase in airway responsiveness to methacholine (Mch) (n=3 separate experiments; 12 mice per group) (P<0.05 versus RSV). The concentration of Mch required to induce a 200% increase in airway responsiveness (PC200) was significantly greater in RSV infected mice who had received the pND-G vaccine compared to RSV infected mice who had not received the vaccine (21.5 mg/ml Mch versus 7.2 mg/ml Mch) (P<0.05) (Miller et al., 2002).