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Vaccine Detail

A. salmonicida live-attenuated vaccine encoding AroA
Vaccine Information
  • Vaccine Ontology ID: VO_0011386
  • Type: Live, attenuated vaccine
  • Status: Research
  • Antigen: A. salmonicida 644Rb aroA
  • aroA gene engineering:
    • Type: Gene mutation
    • Description: The aroA gene of A. salmonicida was cloned in Escherichia coli, and the nucleotide sequence was determined. The aroA gene was inactivated by inserting a fragment expressing kanamycin resistance within the coding sequence. The aroA::Kar mutation was introduced into the chromosome of virulent A. salmonicida 644Rb and 640V2 by allele replacement by using a suicide plasmid delivery system. The mutation resulted in attenuation when bacteria were injected intramuscularly into Atlantic salmon (Vaughan et al., 1993).
    • Detailed Gene Information: Click Here.
  • aroA gene engineering:
    • Type: Gene mutation
    • Description: This aroA mutant is from Aeromonas salmonicida (Vaughan et al., 1993).
    • Detailed Gene Information: Click Here.
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Brown Trout Response

  • Vaccination Protocol: Brown trout were vaccinated with 107 CFU of A. salmonicida 644Rb aroa by intraperitoneal injection (Vaughan et al., 1993).
  • Challenge Protocol: Groups of four or five fish were challenged by intramuscular injection of dilutions of cultures of a virulent strain of A. salmonicida (Vaughan et al., 1993).
  • Efficacy: Vaccination of brown trout with 107 CFU of A. salmonicida 644Rb aroA by intraperitoneal injection resulted in a 253-fold increase in the 50% lethal dose (LD50) compared with unvaccinated controls challenged with a virulent clinical isolate 9 weeks later. A second vaccination after 6 weeks increased the LD50 by a further 16-fold (Vaughan et al., 1993).
References
Vaughan et al., 1993: Vaughan LM, Smith PR, Foster TJ. An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis. Infection and immunity. 1993; 61(5); 2172-2181. [PubMed: 8478107].