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Vaccine Detail

E. histolytica SREHP protein vaccine
Vaccine Information
  • Vaccine Ontology ID: VO_0011416
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: E. histolytica serine-rich protein (SREHP)
  • SREHP gene engineering:
    • Type: Recombinant protein preparation
    • Description: Expressed the SREHP-MBP molecule in an attenuated vaccine strain of Salmonella typhimurium (Zhang and Stanley, 1996).
    • Detailed Gene Information: Click Here.
  • Immunization Route: Oral
Host Response

Gerbil Response

  • Vaccination Protocol: female BALB/c mice or gerbils were deprived of water and food for 4 h and then were given 50 ml of 10% sodium bicarbonate solution per orogastric gavage with a 21-gauge ball-tipped gavage needle. Five minutes later, they received by gavage 50 ml of 0.16Mphosphate-buffered saline (PBS), pH 7.0, containing 109 S. typhimurium cells harboring either pSS3137 (Stm-SREHP) or pYA3137 (Stm-Ctrl). Mice and gerbils were immunized on days 0, 7, and 21. Serum samples were obtained on days 0, 7, 21, and 28 (Zhang and Stanley, 1996).
  • Challenge Protocol: On day 35 (14 days following the final booster immunization) control gerbils vaccinated with Stm-Ctrl and gerbils vaccinated with Stm-SREHP were challenged intrahepatically with 5 x 105 E. histolytica HM1:IMSS trophozoites (Zhang and Stanley, 1996).
  • Efficacy: Report describes a recombinant fusion protein containing the serine-rich Entamoeba histolytica protein (SREHP), a protective antigen derived from virulent amebae, and a bacterially derived maltose-binding protein (MBP) from an attenuated strain of Salmonella typhimurium. Gerbils vaccinated with S typhimurium SREHP-MBP were protected against amebic liver abscess after challenge with E. histolytica, the most common extraintestinal complication of amebiasis (Zhang and Stanley, 1996).
References
Zhang and Stanley, 1996: Zhang T, Stanley SL Jr. Oral immunization with an attenuated vaccine strain of Salmonella typhimurium expressing the serine-rich Entamoeba histolytica protein induces an antiamebic immune response and protects gerbils from amebic liver abscess. Infection and immunity. 1996; 64(5); 1526-1531. [PubMed: 8613356].