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Vaccine Detail

Hepatitis B DNA vaccine pCMV-S2.S encoding the HBV(ayw Strain) envelope protein.
Vaccine Information
  • Vaccine Ontology ID: VO_0004360
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Chimpanzee
  • PreS2+S (Middle protein) gene engineering:
    • Type: DNA vaccine construction
    • Detailed Gene Information: Click Here.
  • S gene engineering:
    • Type: DNA vaccine construction
    • Detailed Gene Information: Click Here.
  • Vector: pcDNA3 (Davis et al., 1996)
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Chimpanzee Response

  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: Immunization of chimpanzees with HBsAg-expressing plasmid DNA induced specific anti-HBs antibodies. The higher dose of DNA (2 mg) induced significant titers (>100 mIU/ml) of anti-HBs after the initial injection of DNA. These titers never went below the 10 mIU/ml level considered adequate to confer protection (Davis et al., 1996).
  • Efficacy: The outcome of challenge with live HBV of the ayw strain was strongly correlated with the anti-HBs titers. Of the eight chimpanzees with a titer >10 mIU/ml, all were protected from infection except for one, which had the lowest anti-HBs titer at 12 mIU/ml. These findings agree closely with the critical protective level of 10 mIU/ml determined for humans by the Centers for Disease Control (Davis et al., 1996).
References
Davis et al., 1996: Davis HL, McCluskie MJ, Gerin JL, Purcell RH. DNA vaccine for hepatitis B: evidence for immunogenicity in chimpanzees and comparison with other vaccines. Proceedings of the National Academy of Sciences of the United States of America. 1996; 93(14); 7213-7218. [PubMed: 8692971].