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Vaccine Detail

Hepatitis B virus DNA vaccine pVAX-PS
Vaccine Information
  • Vaccine Ontology ID: VO_0011404
  • Type: DNA vaccine
  • Status: Research
  • Antigen: Hepatitis B virus preS2 middle surface protein
  • envelope gene engineering:
    • Type: DNA vaccine construction
    • Description: pVAXPS was constructed by inserting the gene encoding the middle (pre-S2 plus S) envelope protein of HBV into a plasmid vector pVAX1 (Zhou et al., 2003).
    • Detailed Gene Information: Click Here.
  • Vector: Plasmid vector pVAX1
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Tree shrew Response

  • Vaccination Protocol: Sixty adult tree shrews purchased from the Kunming Animal Institute were randomly divided into four groups and immunized twice at 2-week intervals with the DNA vaccine by i.m. injection of 100 mg of pVAX-PS or pVAX1 in a volume of 100 ml in bilateral quadriceps (Zhou et al., 2003).
  • Challenge Protocol: Two weeks after the second DNA immunization, tree shrews in two groups (immunized with pVAX-PS and pVAX1, respectively) were challenged through the caudal vein with 0.8 ml of the patient’s serum positive for the HBV marker (Zhou et al., 2003).
  • Efficacy: The immunological protection of pVAX-PS, a DNA vaccine, was assessed in the tree shrews model. pVAX-PS was constructed by inserting the gene encoding the middle (pre-S2 plus S) envelope protein of HBV into a plasmid vector pVAX1. Results indicated that pVAX-PS immunization could induce remarkable humoral immune response and prevent the experimental tree shrews from infection of HBV (Zhou et al., 2003).
References
Zhou et al., 2003: Zhou FJ, Hu ZL, Dai JX, Chen RW, Shi K, Lin Y, Sun SH. Protection of tree shrews by pVAX-PS DNA vaccine against HBV infection. DNA and cell biology. 2003; 22(7); 475-478. [PubMed: 12932306].