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Vaccine Comparison

Aeromonas hydrophila LSA34 vector vaccine WED/pUTDgap Recombinant Aeromonas hydrophila vaccine (Aera)
Vaccine Information Vaccine Information
  • Vaccine Ontology ID: VO_0004606
  • Type: Recombinant vector vaccine
  • Status: Research
  • Antigen: The protective antigen GAPDH from the fish pathogen A. hydrophila, encoded by the gene gapA34 (Mu et al., 2011).
  • Vector: The Edwardsiella tarda strain EIB202 is used a a live, attenuated vaccine vector, using the promoter P(dps) as the protein-based expression system for the antigen (Mu et al., 2011).
  • Preparation: The gapA34 gene was introduced into P(dps) and transformed into live, attenuated E. tarda strain EIB202 (Mu et al., 2011).
  • Immunization Route: Intramuscular injection (i.m.)
  • Description: A. hydrophila is a common fish pathogen, so in order to create a vaccine against this pathogen, the gapA34 gene of A. hydrophila was expressed in the bacterial vector E. tarda strain EIB202, and it was shown to increase survival of vaccinated fish (Mu et al., 2011).
  • Vaccine Ontology ID: VO_0004811
  • Type: Recombinant vector vaccine
  • Status: Licensed
  • Host Species for Licensed Use: Baboon
  • Immunization Route: Intramuscular injection (i.m.)
Host Response Host Response

Fish Response

  • Host Strain: Turbot (Scophtalmus maximus)
  • Vaccine Immune Response Type: VO_0000287
  • Efficacy: Over 60% of the fish vaccinated with the WED/pUTDgap vector vaccine survived the A. hydrophila challenge (Mu et al., 2011).
  • Description: The P(dps) promoter--which was chosen due to its high transcription activity-- was shown to elicit survival in turbot, meaning that it has the potential to be an important part of bacterial vaccine vector application (Mu et al., 2011) .

Fish Response

  • Vaccination Protocol: In vaccination experiments, healthy grass carp were distributed randomly into control group and bath immunization group (150 fish per group). For bath immunization, fish were immersed in 20 mg L−1 SWCNTs-Aera and the control group was immersed in PBS for 6 h. At the end of the vaccination, grass carp were transferred to different tanks without SWCNTs-Aera for 28 d. After 28 d, 15 fish in each replicate were euthanized in the laboratory through washrag soaked with 20.0 g/m3 MS-222. Sampled fish were dissected immediately with sterile scissors. The intestine, kidney and spleen were aseptically removed from their abdominal cavity, separately, where five fish were sampled for analyzing expression of seven immune-related genes through quantitative real-time PCR (qRT-PCR) and the experiment was performed in triplicate replicates. Thereafter, the intestine of three fish (15 fish per replicate) were also collected and pooled together as described elsewhere to extract microbial DNA for deep sequencing [29]. After 2 d, the remaining fish (15 grass carp in each replicate) were intramuscularly injected with 6.5 × 104 cfu mL−1A. hydrophila, kept for 10 days and thereafter euthanized for sampling. Subsequently, the intestine was carefully excised and maintained as above, which was also for extracting microbial DNA for deep sequencing. All the samples were placed into sterile polypropylene centrifuge tubes and stored in −80 °C until DNA extraction. All experiments were performed in triplicate (Liu et al., 2015).
  • Host CD8A response
    • Description: The expression of CD8A was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
    • Detailed Gene Information: Click Here.
  • Host IFN1 response
    • Description: The expression of IFN1 was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
    • Detailed Gene Information: Click Here.
  • Host IgM response
    • Description: The expression of IgM was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
    • Detailed Gene Information: Click Here.
  • Host IL8 response
    • Description: The expression of IL8 was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
    • Detailed Gene Information: Click Here.
  • Host TNF-alpha response
    • Description: The expression of TNF-A was upregulated by the Aera in the intestine, kidney and spleen (Liu et al., 2015).
    • Detailed Gene Information: Click Here.
References References
Mu et al., 2011: Mu W, Guan L, Yan Y, Liu Q, Zhang Y. A novel in vivo inducible expression system in Edwardsiella tarda for potential application in bacterial polyvalence vaccine. Fish & shellfish immunology. 2011; 31(6); 1097-1105. [PubMed: 21964456].
Liu et al., 2015: Liu L, Gong YX, Zhu B, Liu GL, Wang GX, Ling F. Effect of a new recombinant Aeromonas hydrophila vaccine on the grass carp intestinal microbiota and correlations with immunological responses. Fish & shellfish immunology. 2015; ; . [PubMed: 25862971].