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Vaccine Detail

Yellow Fever NS1 Protein Vaccine
Vaccine Information
  • Vaccine Ontology ID: VO_0004151
  • Type: Subunit vaccine
  • Status: Research
  • NS1 (non-structural protein 1) gene engineering:
    • Type: Purified protein
    • Description: Immunoaffinity-purified NS1 was prepared from lysates of 17D YF-infected Vero cells (Schlesinger et al., 1986).
    • Detailed Gene Information: Click Here.
  • Adjuvant: aluminum vaccine adjuvant
  • Immunization Route: Intradermal injection (i.d.)
Host Response

Monkey Response

  • Host Strain: Rhesus
  • Vaccination Protocol: Five rhesus monkeys were given combined intradermal and subcutaneous injections of NS1 adsorbed to an alum adjuvant. Four monkeys injected in parallel with ovalbumin in alum served as controls (Schlesinger et al., 1986).
  • Challenge Protocol: Animals were injected initially with 100 μg and given booster injections of 50 μg of NS1 or ovalbumin at 3 and at 7 weeks; they were challenged 1 to 2 weeks after the second booster injection by subcutaneous inoculation of a lethal dose (375 to 700 PFU) of African strain Dakar 1279 Yellow Fever. Subcutaneous inoculation of 50 to 200 PFU of this virus is uniformly lethal for rhesus monkeys (Schlesinger et al., 1986).
  • Efficacy: All four control monkeys died, as expected, whereas four of the five
    immunized monkeys survived lethal YF viral challenge (Schlesinger et al., 1986).
References
Schlesinger et al., 1986: Schlesinger JJ, Brandriss MW, Cropp CB, Monath TP. Protection against yellow fever in monkeys by immunization with yellow fever virus nonstructural protein NS1. Journal of virology. 1986; 60(3); 1153-1155. [PubMed: 3783816].