• Vaccine Ontology ID: VO_0004187
• Type: Recombinant vector vaccine
• Status: Licensed
• H hemagglutinin protein gene engineering:
  • Type: Recombinant vector construction
  • Detailed Gene Information: Click Here.
• Vector: Chimeric Venezuelan equine encephalitis/Sindbis virus (VEE/SIN) replicon particles (Pan et al., 2010).
• Immunization Route: Intradermal injection (i.d.)

• Vaccine Ontology ID: VO_0011422
• Type: Recombinant vector vaccine
• Status: Research
• F fusion protein gene engineering:
  • Type: Recombinant protein preparation
  • Detailed Gene Information: Click Here.
• H hemagglutinin protein gene engineering:
  • Type: Recombinant protein preparation
  • Detailed Gene Information: Click Here.
• Vector: chimeric Venezuelan equine encephalitis/Sindbis virus (VEE/SIN) replicon particles (Pan et al., 2010).
• Immunization Route: Intradermal injection (i.d.)

Monkey Response

• Host Strain: Rhesus macaques
• Vaccination Protocol: Juvenile rhesus macaques were vaccinated with a single intradermal (i.d.) injection of 10^8 VEE/SIN-H particles or 108 VEE/SIN-H particles plus 3 x 10^6 VEE/SIN-F particles (VEE/SIN-H+F). Two juvenile monkeys were vaccinated with one dose of 5,000 PFU of the Moraten strain of LAV intramuscularly (i.m.). Two juvenile monkeys were vaccinated with three doses of 0.5 ml of FIMV i.m. at 0, 4, and 8 weeks. Three infant rhesus macaques were immunized i.d. with 10^8 VEE/SIN-H particles plus 3 x 10^6 VEE/SIN-F particles and boosted 2 months later (Pan et al., 2010)
• Challenge Protocol: For MV challenge, 10^4 tissue culture 50% infectious doses (TCID50) of the Bilthoven strain of MV were instilled intratracheally into anesthetized animals 12 to 17 months after vaccination. Monkeys were shaved and monitored frequently for development of a rash (Pan et al., 2010).
• Efficacy: Neutralizing antibody remained above the protective level for more than 1 year after vaccination with VEE/SIN-H, VEE/SIN-H+F, or LAV. When challenged with wild-type MV 12 to 17 months after vaccination, all vaccinated juvenile and infant monkeys vaccinated with VEE/SIN-H, VEE/SIN-H+F, and LAV were protected from rash and viremia, while FIMV-vaccinated monkeys were not (Pan et al., 2010).

Monkey Response

• Host Strain: Rhesus macaques
• Vaccination Protocol: Juvenile rhesus macaques were vaccinated with a single intradermal (i.d.) injection of 10^8 VEE/SIN-H particles or 10^8 VEE/SIN-H particles plus 3 x 10^6 VEE/SIN-F particles (VEE/SIN-H+F). Two juvenile monkeys were vaccinated with one dose of 5,000 PFU of the Moraten strain of LAV intramuscularly (i.m.). Two juvenile monkeys were vaccinated with three doses of 0.5 ml of FIMV i.m. at 0, 4, and 8 weeks. Three infant rhesus macaques were immunized i.d. with 10^8 VEE/SIN-H particles plus 3 x 10^6 VEE/SIN-F particles and boosted 2 months later (Pan et al., 2010).
• Challenge Protocol: For MV challenge, 10^4 tissue culture 50% infectious doses (TCID50) of the Bilthoven strain of MV were instilled intratracheally into anesthetized animals 12 to 17 months after vaccination. Monkeys were shaved and monitored frequently for development of a rash (Pan et al., 2010).
• Efficacy: Neutralizing antibody remained above the protective level for more than 1 year after vaccination with VEE/SIN-H, VEE/SIN-H+F, or LAV. When challenged with wild-type MV 12 to 17 months after vaccination, all vaccinated juvenile and infant monkeys vaccinated with VEE/SIN-H, VEE/SIN-H+F, and LAV were protected from rash and viremia, while FIMV-vaccinated monkeys were not (Pan et al., 2010).
• Host IFNG response
  • Description: VEE/SIN-H+F vaccinated rhesus macaques showed a significant increase in IFN-gamma producing cells in blood 1 to 2 weeks after vaccination as compared to the current live attenuated virus vaccine (LAV). The significant increase came after stimulation with the measles H-peptide (Pan et al., 2010).
  • Detailed Gene Information: Click Here.