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Vaccine Detail

Laryngotracheitis DNA Vaccine encoding gB
Vaccine Information
  • Vaccine Ontology ID: VO_0004565
  • Type: DNA vaccine
  • Status: Research
  • gB gene engineering:
    • Type: DNA vaccine construction
    • Detailed Gene Information: Click Here.
  • Vector: pcDNA3.1(+) (Chen et al., 2011)
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Baboon Response

  • Vaccination Protocol: At 21 days of age, the chickens were randomly divided into four groups of 20 chickens each. Chickens in group 1 were administered 150 μg plasmid pgB alone by intramuscular injection into the quadriceps muscle. Chickens in group 2 were immunized with 150 μg plasmid pgB plus 150 μg plasmid pIL-18. Chickens in group 3, which served as a negative-control group, were immunized with 150 μg plasmid pcDNA3.1(+). Chickens in group 4 were immunized with 0.5 ml sterile PBS alone as a blank control (Chen et al., 2011).
  • Vaccine Immune Response Type: VO_0003057
  • Immune Response: Compared with the chickens in the pcDNA3.1(+) and PBS group, the chickens in the pgB with or without pIL-18 groups showed significantly increased levels of anti-ILTV antibodies with subsequent vaccination. In addition, an enhanced T-cell proliferative response to gB was clearly observed in the groups immunized with pgB alone or with pgB plus pIL-18 (Chen et al., 2011).
  • Challenge Protocol: At 15 days after the booster immunization, all of the chickens were challenged intratracheally with 150 EID50 virulent ILTV CG strain (Chen et al., 2011).
  • Efficacy: The protection rates in the pgB and pgB plus pIL-18 groups were 60 and 80 %, respectively (Chen et al., 2011).
References
Chen et al., 2011: Chen HY, Zhang HY, Li XS, Cui BA, Wang SJ, Geng JW, Li K. Interleukin-18-mediated enhancement of the protective effect of an infectious laryngotracheitis virus glycoprotein B plasmid DNA vaccine in chickens. Journal of medical microbiology. 2011; 60(Pt 1); 110-116. [PubMed: 20829398].