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Vaccine Detail

Heat-killed Virulent Shigella flexneri 2a
Vaccine Information
  • Vaccine Ontology ID: VO_0000672
  • Type: Inactivated or "killed" vaccine
  • Antigen: Heat-killed shigella flexneri strain 2a (Chakrabarti et al., 1999).
  • Preparation: Each vacccine consisted of 10 ml of heat-killed bacteria, and each contained 1011 CFU of bacteria (Chakrabarti et al., 1999).
  • Virulence: Protection from diarrhea and dysentary following oral immunization was 100% .
Host Response

Rabbit Response

  • Host Strain: New Zealand white
  • Vaccination Protocol: 25 male New Zealand white rabbits were given 10 ml of broth culture containing 1011 CFU of the heat-killed bacteria. The vaccines were administered through injection of the colon. The rabbits were imminized for seven days, and were administered a total of 5 doses (Chakrabarti et al., 1999).
  • Persistence: Not noted.
  • Immune Response: The immune response can only be speculated upon, but it is believed that protective immunity to Shigella may by conferred by serum IgG antibodies to the O-specific polysaccaride of their lipopolysaccarides. There is also evidence that infection also confers type-specific immunity to LPS (Chakrabarti et al., 1999).
  • Side Effects: Side effects included bloody diarrhea and dysentary (Chakrabarti et al., 1999).
  • Challenge Protocol: Challenge specifics were not specifically noted.
  • Efficacy: Challenge was only demonstrated using the same strain present inthe vaccine, and was eveidenced by lack of diarrhea. Death following challenge was 2.5 fold higher for non-immunized rabbits, but this was not significant (Chakrabarti et al., 1999).
References
Chakrabarti et al., 1999: Chakrabarti MK, Bhattacharya J, Bhattacharya MK, Nair GB, Bhattacharya SK, Mahalanabis D. Killed oral Shigella vaccine made from Shigella flexneri 2a protects against challenge in the rabbit model of shigellosis. Acta paediatrica (Oslo, Norway : 1992). 1999; 88(2); 161-165. [PubMed: 10102148].