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Vaccine Detail

Bordetella bronchiseptica dnt mutant vaccine
Vaccine Information
  • Vaccine Ontology ID: VO_0002805
  • Type: Live, attenuated vaccine
  • Status: Research
  • dnt gene engineering:
    • Type: Gene mutation
    • Detailed Gene Information: Click Here.
  • Preparation: Dom+ Scs+ Hly+ organisms of strain L3 were streaked on two BG-20 agar plates and incubated at 37°C. Ten Dom+ Scs+ Hly- colonies (0.5 to 0.7mm in diameter) grown on each plate were subcultured 10 times under the same culture conditions. Finally, four mutant strains showing the Dom+ Scs+ Hly -phenotype and lacking DNT- producing ability were obtained (Nagano et al., 1988).
  • Immunization Route: intranasal immunization
Host Response

Guinea pig Response

  • Efficacy: Most of the guinea pigs inoculated intranasally or intramuscularly with 2.3 x 107 or 2.3 x 108 viable cells of strain B-42 produced serum agglutination antibodies at 3 week after the inoculation, and most of them survived intranasal challenge with strain L3 (770 x LD50) during observation period (Nagano et al., 1988).
References
Nagano et al., 1988: Nagano H, Nakai T, Horiguchi Y, Kume K. Isolation and characterization of mutant strains of Bordetella bronchiseptica lacking dermonecrotic toxin-producing ability. Journal of clinical microbiology. 1988; 26(10); 1983-1987. [PubMed: 3182989].