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Vaccine Comparison

M. gallisepticum TM-1 Protein Subunit Vaccine Mycoplasma gallisepticum lpd mutant vaccine
Vaccine Information Vaccine Information
  • Vaccine Ontology ID: VO_0011515
  • Type: Subunit vaccine
  • Status: Research
  • TM-1 gene engineering:
    • Type: Recombinant protein preparation
    • Detailed Gene Information: Click Here.
  • Adjuvant: aluminum hydroxide vaccine adjuvant
  • Immunization Route: Subcutaneous injection
  • Vaccine Ontology ID: VO_0002857
  • Type: Live, attenuated vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Chicken
  • lpd gene engineering:
    • Type: Gene mutation
    • Description: This lpd mutant is from Mycoplasma gallisepticum (Gates et al., 2008).
    • Detailed Gene Information: Click Here.
  • Immunization Route: Intratracheal immunization
Host Response Host Response

Chicken Response

  • Host Strain: SPF
  • Vaccination Protocol: Eight-week-old specific pathogen-free (SPF) chickens were immunized subcutaneously in the right thigh with the TM-1 polypeptide, suspended in a mixture of 1 ml of PBS and 1 ml of an aluminium hydroxide adjuvant (Saito et al., 1993).
  • Challenge Protocol: Four weeks later, the immunized chickens were challenged intranasally with 0.5 ml of a suspension of 106 c.f.u, ml- 1 of M.g. KP13 strain 27 (Saito et al., 1993).
  • Efficacy: Only one of five immunized chickens harboured viable M.g. cells in its sinuses after being killed 5 days after challenge with M.g. (Saito et al., 1993).

Chicken Response

  • Persistence: An lpd mutant is highly attenuated in chickens (Gates et al., 2008).
  • Efficacy: An lpd mutant induces significant protection in chickens from challenge with wild type Mycoplasma gallisepticum (Gates et al., 2008).
  • Host IgG Fc fragment response
    • Description: Mg 7 vaccinated chickens mounted a strong humoral anti-M. gallisepticum-specific IgG response that was sustained for at least 4 weeks post-exposure. M. gallisepticum-specific immunoglobulin responses were detected in chickens inoculated with either Mg 7 or R(low) but not in chickens that received 1× PBS alone. The level of M. gallisepticum-specific serum IgG was highest in the R(low) treated animals at both the 2- and 4-week time points, however, the Mg 7 inoculated birds mounted a fairly robust response considering its level of attenuation. Thus, the Mg 7 vaccine induced significantly higher IgG responses than PBS inoculated chickens, but significantly lower than those inoculated with wild type R(low) (Gates et al., 2008).
    • Detailed Gene Information: Click Here.
References References
Saito et al., 1993: Saito S, Fujisawa A, Ohkawa S, Nishimura N, Abe T, Kodama K, Kamogawa K, Aoyama S, Iritani Y, Hayashi Y. Cloning and DNA sequence of a 29 kilodalton polypeptide gene of Mycoplasma gallisepticum as a possible protective antigen. Vaccine. 1993; 11(10); 1061-1066. [PubMed: 8212828].
Gates et al., 2008: Gates AE, Frasca S, Nyaoke A, Gorton TS, Silbart LK, Geary SJ. Comparative assessment of a metabolically attenuated Mycoplasma gallisepticum mutant as a live vaccine for the prevention of avian respiratory mycoplasmosis. Vaccine. 2008; 26(16); 2010-2019. [PubMed: 18342996].