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Avian influenza DNA vaccine pCMV-M |
Avian influenza virus DNA vaccine pCMV-HA |
Influenza virus DNA vaccine p188 encoding H7 |
Influenza virus DNA vaccine pCAGGHA |
Influenza virus DNA vaccine pCAGGoptiHA |
Influenza virus DNA vaccine pCMVH5HA |
Influenza virus DNA vaccine pβactinH7HA |
pCAGGoptiHA |
| Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
- Vaccine Ontology ID: VO_0004504
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Chicken
- M1
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pcDNA 3.1 expressed M1 viral proteins from an Italian H7N1 LPAI virus (Le et al., 2007).
- Detailed Gene Information: Click Here.
- Vector: pcDNA 3.1 (Le et al., 2007)
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0004505
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Chicken
- ha
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pcDNA 3.1 expressed H7 viral proteins from an Italian H7N1 LPAI virus (Le et al., 2007).
- Detailed Gene Information: Click Here.
- Vector: pcDNA 3.1 (Le et al., 2007)
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0004291
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Chicken
- HA
gene engineering:
- Type: DNA vaccine construction
- Description: Vector p188 expressed a replication-defective derivative of avian leucosis virus that expresses the haemagglutinin type 7 (HT) gene of A/Seal/Mass/I/80 (H7N7) (Robinson et al., 1993).
- Detailed Gene Information: Click Here.
- Vector: p188 (Robinson et al., 1993).
- Immunization Route: Intraperitoneal injection (i.p.)
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- Vaccine Ontology ID: VO_0004503
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Chicken
- HA from Influenza A virus (A/Goose/Guangdong/1/96(H5N1))
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pCAGGS expressed chicken biased codons based on the HA amino acid sequence of the highly pathogenic H5N1 virus A/goose/Guangdong/1/96 (GS/GD/96) (Jiang et al., 2007).
- Detailed Gene Information: Click Here.
- Vector: pCAGGS (Jiang et al., 2007)
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0004502
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Chicken
- HA from Influenza A virus (A/Goose/Guangdong/1/96(H5N1))
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pCAGGS expressed chicken biased codons based on the HA amino acid sequence of the highly pathogenic H5N1 virus A/goose/Guangdong/1/96 (GS/GD/96) (Jiang et al., 2007).
- Detailed Gene Information: Click Here.
- Vector: pCAGGS (Jiang et al., 2007)
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0004500
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Chicken
- HA
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pCMV expressed a full-length cDNA copy of the HA gene of Ty/Ire/83 (Kodihalli et al., 2000).
- Detailed Gene Information: Click Here.
- Vector: pCMV (Kodihalli et al., 2000)
- Immunization Route: Gene gun
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- Vaccine Ontology ID: VO_0004501
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Chicken
- HA
gene engineering:
- Type: DNA vaccine construction
- Description: This vaccine also expressed HA from A/Chick/Victoria/1/85 (H7N7) (Kodihalli et al., 2000).
- Detailed Gene Information: Click Here.
- Vector: pCMV (Kodihalli et al., 2000)
- Immunization Route: Gene gun
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- Vaccine Ontology ID: VO_0004173
- Type: DNA vaccine
- Status: Research
- Antigen: Influenza A virus (A/Goose/Guangdong/1/96(H5N1)) HA hemagglutinin
- HA from Influenza A virus (A/Goose/Guangdong/1/96(H5N1))
gene engineering:
- Type: DNA vaccine construction
- Description: Both the wild-type HA gene of GS/GD/96 virus and optiHA were cloned into the plasmid vector pCAGGS (kindly provided by Dr. Y. Kawaoka) under the control of the chicken β-actin promoter, and plasmid pCI (Promega) under the control of the cytomegalovirus (CMV) promoter (Jiang et al., 2007).
- Detailed Gene Information: Click Here.
- Vector: plasmid vector pCAGGS
- Immunization Route: Intramuscular injection (i.m.)
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| Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
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Chicken Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: After the homologous challenge, chickens of every vaccinated group displayed a significant decrease in cloacal shedding, whereas tracheal shedding was not significantly reduced in the protein/protein group (Le et al., 2007).
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Chicken Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: After the homologous challenge, chickens of every vaccinated group displayed a significant decrease in cloacal shedding, whereas tracheal shedding was not significantly reduced in the protein/protein group (Le et al., 2007).
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Chicken Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: In a series of four experiments, 50% (28/56) of the DNA-vaccinated and < 2% (1/67) of the control chickens survived the challenge (Robinson et al., 1993).
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Chicken Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: All chickens receiving pCAGGHA developed high levels of HI and NT antibodies at 3 weeks p.v., and were completely protected from lethal H5 virus challenge (Jiang et al., 2007).
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Chicken Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: All chickens receiving pCAGGoptiHA developed high levels of HI and NT antibodies at 3 weeks p.v., and were completely protected from lethal H5 virus challenge (Jiang et al., 2007).
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Chicken Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: Chickens given a single dose of plasmids expressing H5 and H7 hemagglutinins protected the birds from infection by either subtype (Kodihalli et al., 2000).
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Chicken Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: Chickens given a single dose of plasmids expressing H5 and H7 hemagglutinins protected the birds from infection by either subtype (Kodihalli et al., 2000).
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Chicken Response
- Vaccination Protocol: The plasmids were diluted to the desired concentration and were administered by intramuscular injection in a 200 μl volume at the leg muscle (Jiang et al., 2007).
- Challenge Protocol: Chickens were challenged with 1000 LD50 of H5N1 viruses in 0.1 ml intranasally. Oropharyngeal and cloacal swabs were collected from all chickens at 3, 5 and 7 days post-challenge (p.c.) for virus titration in eggs, and chickens were observed daily for disease signs and death for 2 weeks (Jiang et al., 2007).
- Efficacy: Results indicated that two doses of 10 microg of pCAGGoptiHA derived from Influenza A virus (A/Goose/Guangdong/1/96(H5N1)) could induce complete protection in chickens against H5 lethal virus challenge (Jiang et al., 2007).
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| References |
References |
References |
References |
References |
References |
References |
References |
Le et al., 2007: Le Gall-Reculé G, Cherbonnel M, Pelotte N, Blanchard P, Morin Y, Jestin V. Importance of a prime-boost DNA/protein vaccination to protect chickens against low-pathogenic H7 avian influenza infection. Avian diseases. 2007; 51(1 Suppl); 490-494. [PubMed: 17494616].
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Le et al., 2007: Le Gall-Reculé G, Cherbonnel M, Pelotte N, Blanchard P, Morin Y, Jestin V. Importance of a prime-boost DNA/protein vaccination to protect chickens against low-pathogenic H7 avian influenza infection. Avian diseases. 2007; 51(1 Suppl); 490-494. [PubMed: 17494616].
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Robinson et al., 1993: Robinson HL, Hunt LA, Webster RG. Protection against a lethal influenza virus challenge by immunization with a haemagglutinin-expressing plasmid DNA. Vaccine. 1993; 11(9); 957-960. [PubMed: 8212843].
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Jiang et al., 2007: Jiang Y, Yu K, Zhang H, Zhang P, Li C, Tian G, Li Y, Wang X, Ge J, Bu Z, Chen H. Enhanced protective efficacy of H5 subtype avian influenza DNA vaccine with codon optimized HA gene in a pCAGGS plasmid vector. Antiviral research. 2007; 75(3); 234-241. [PubMed: 17451817].
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Jiang et al., 2007: Jiang Y, Yu K, Zhang H, Zhang P, Li C, Tian G, Li Y, Wang X, Ge J, Bu Z, Chen H. Enhanced protective efficacy of H5 subtype avian influenza DNA vaccine with codon optimized HA gene in a pCAGGS plasmid vector. Antiviral research. 2007; 75(3); 234-241. [PubMed: 17451817].
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Kodihalli et al., 2000: Kodihalli S, Kobasa DL, Webster RG. Strategies for inducing protection against avian influenza A virus subtypes with DNA vaccines. Vaccine. 2000; 18(23); 2592-2599. [PubMed: 10775793].
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Kodihalli et al., 2000: Kodihalli S, Kobasa DL, Webster RG. Strategies for inducing protection against avian influenza A virus subtypes with DNA vaccines. Vaccine. 2000; 18(23); 2592-2599. [PubMed: 10775793].
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Jiang et al., 2007: Jiang Y, Yu K, Zhang H, Zhang P, Li C, Tian G, Li Y, Wang X, Ge J, Bu Z, Chen H. Enhanced protective efficacy of H5 subtype avian influenza DNA vaccine with codon optimized HA gene in a pCAGGS plasmid vector. Antiviral research. 2007; 75(3); 234-241. [PubMed: 17451817].
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