• Vaccine Ontology ID: VO_0004533
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Chickens
• VP2 gene engineering:
  • Type: DNA vaccine construction
  • Description: The synthetic gene for VP2 protein of vvIBDV Gx with codons optimized for chicken usage was synthesized and cloned into pCAGGS vector (Gao et al., 2013).
  • Detailed Gene Information: Click Here.
• Vector: pCAGGS prime, rVP2 protein boost (Gao et al., 2013)
• Immunization Route: Intramuscular injection (i.m.)

• Vaccine Ontology ID: VO_0004534
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Chickens
• Antigen: VP243 gene of vvIBDV HLJ0504 (VP243 can be cleaved into VP2, VP3, and VP4) (Li et al., 2013)
• VP2-VP4-VP3 gene engineering:
  • Type: DNA vaccine construction
  • Detailed Gene Information: Click Here.
• Vector: pCAGGS (Li et al., 2013)
• Immunization Route: Intramuscular injection (i.m.)

• Vaccine Ontology ID: VO_0004585
• Type: DNA vaccine
• Status: Research
• VP2 gene engineering:
  • Type: DNA vaccine construction
  • Detailed Gene Information: Click Here.
• Vector: pIRES (Kumar et al., 2009)
• Immunization Route: Intramuscular injection (i.m.)

Chicken Response

• Immune Response: Chickens in the DNA prime–protein boost group developed significantly higher levels of ELISA and neutralizing antibodies to IBDV compared with those immunized with either the DNA vaccine or the protein vaccine alone (P < 0.05). Furthermore, the highest levels of lymphocyte proliferation response, IL-4 and IFN-γ production were induced following priming with the DNA vaccine and boosting with the rVP2 protein (Gao et al., 2013).
• Efficacy: Chickens inoculated with the DNA prime–protein boost vaccine had 100% protection against challenge with vvIBDV, as evidenced by the absence of clinical signs, mortality, and bursal atrophy. In contrast, chickens receiving the DNA vaccine and the rVP2 protein vaccine had 67% and 80% protection, respectively (Gao et al., 2013).

Chicken Response

• Immune Response: Chickens immunized with plasmid pCAGVP243-IL-18 carrying both VP243 and IL-18 genes induced significantly higher levels of antibodies, lymphocyte proliferation responses and of the cytokines IL-4 and IFN-γ than those injected with pCAGVP243 encoding the VP243 gene alone (Li et al., 2013).
• Efficacy: Furthermore, pCAGVP243-IL-18 provided higher protection (93%) against vvIBDV challenge in chickens than pCAGVP243 (60%), as evidenced by the absence of clinical signs, mortality, and bursal atrophy (Li et al., 2013).

Chicken Response

• Vaccination Protocol: The birds were immunized intramuscularly with 50 μg plasmid DNA in the quadricep muscle of the hind limb (Kumar et al., 2009).
• Vaccine Immune Response Type: VO_0003057
• Immune Response: Birds immunized with pibdVP2-IRES-chiIL-2 plasmid at 21 days post-challenge showed the highest antibody titer. In addition, blood analysis showed a proliferation of CD4-positive cells in the group vaccinated with pibdVP2-IRES-chiIL-2 (Kumar et al., 2009).
• Challenge Protocol: All vaccinated birds and the unvaccinated control birds were challenged with 10^5 ELD50 (50% embryo lethal dose) virulent IBDV (strain KT1/99) through the intrabursal and intraocular routes at 21 days post-immunization (Kumar et al., 2009).
• Efficacy: Birds vaccinated with the pibdVP2-IRES-chiIL-2 plasmid showed an 80% protection efficacy (Kumar et al., 2009).