|
Newcastle disease virus DNA vaccine pCAGF encoding the F protein |
Newcastle Disease Virus HN Glycoprotein Subunit Vaccine |
| Vaccine Information |
Vaccine Information |
- Vaccine Ontology ID: VO_0004324
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Chicken
- F fusion protein
gene engineering:
- Type: DNA vaccine construction
- Description: Vector Pcaggs expressed the Newcastle disease virus Fprotein (NDV-F) (Sakaguchi et al., 1996).
- Detailed Gene Information: Click Here.
- Vector: Pcaggs (Sakaguchi et al., 1996)
- Immunization Route: Intramuscular injection (i.m.)
|
- Vaccine Ontology ID: VO_0011552
- Type: Subunit vaccine
- Status: Research
- Antigen: Hemagglutinin-neuraminidase (HN) glycoprotein (Lee et al., 2008).
- HN hemagglutinin-neuraminidase
gene engineering:
- Type: Recombinant protein preparation
- Detailed Gene Information: Click Here.
- Adjuvant:
- Vector: Baculovirus (Lee et al., 2008).
- Immunization Route: Intramuscular injection (i.m.)
|
| Host Response |
Host Response |
|
Chicken Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: At 9 weeks post-injection, chickens were challenged with the velogenic NDV Sato strain. The chickens that had the antibody against NDV-F from immunization were protected from lethal NDV challenge. The DNA vaccine conferred efficient protection against the disease (Sakaguchi et al., 1996).
|
Chicken Response
- Vaccination Protocol: For vaccination, chickens were inoculated intramuscularly with the Seppic-adjuvanted antigen (Lee et al., 2008).
- Challenge Protocol: Each group of vaccinated and unvaccinated chickens was challenged with the vvNDV Kr-005/00 strain through an intraocular inoculation with an 10^5.5 EID50 per bird. vvNDV Kr-005/00 was the representative strain of genotype VII, which is the dominant epizootic genotype in Korea. The chickens were kept under observation for 14 days after infection (Lee et al., 2008).
- Efficacy: A single dose of rNDHN(V) had a protective effect against mortality (p <0.01) in chickens (Lee et al., 2008).
|
| References |
References |
Sakaguchi et al., 1996: Sakaguchi M, Nakamura H, Sonoda K, Hamada F, Hirai K. Protection of chickens from Newcastle disease by vaccination with a linear plasmid DNA expressing the F protein of Newcastle disease virus. Vaccine. 1996; 14(8); 747-752. [PubMed: 8817820].
|
Lee et al., 2008: Lee YJ, Sung HW, Choi JG, Lee EK, Yoon H, Kim JH, Song CS. Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutininneuraminidase proteins. Journal of veterinary science. 2008; 9(3); 301-308. [PubMed: 18716451].
|
