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Vaccine Comparison

Duck hepatitis B DNA vaccine pcDNA I-S encoding small S proteins Hepatitis B virus DNA vaccine encoding HBVgp2 pre-S1/pre-S2/S
Vaccine Information Vaccine Information
  • Vaccine Ontology ID: VO_0004353
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Duck
  • Antigen: S protein from DHBV Australian strain (Triyatni et al., 1998)
  • S gene engineering:
    • Type: DNA vaccine construction
    • Detailed Gene Information: Click Here.
  • Vector: pcDNA I/Amp (Triyatni et al., 1998)
  • Immunization Route: Intramuscular injection (i.m.)
  • Vaccine Ontology ID: VO_0011411
  • Type: DNA vaccine
  • Status: Research
  • Antigen: Hepatitis B virus HBVgp2 pre-S1/pre-S2/S
  • S gene engineering:
    • Type: DNA vaccine construction
    • Description: The pCI expression vector (Promega, Charbonières, France) was used to clone the entire DHBV large envelope gene into Not I polylinker site leading to the pCI-preS/S plasmid [5] and the DHBV core gene leading to pCI-C plasmid (Thermet, submitted). The pCI-preS/S, pCI-C and the native pCI plasmids were purified by Endotoxin Free Giga prep (Qiagen, Hilden, Germany) (Thermet et al., 2003).
    • Detailed Gene Information: Click Here.
  • Vector: pCI expression vector (Promega, Charbonières, France)
  • Immunization Route: Intramuscular injection (i.m.)
Host Response Host Response

Ducks Response

  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: The S-DNA vaccine was able to elicit humoral immune responses against DHBV surface proteins in ducks. In addition, The S-DNA vaccine induced high titers of anti-DHBs antibodies, and anti-S antibodies induced by the S-DNA construct were highly effective in neutralizing virus infectivity. (Triyatni et al., 1998).
  • Efficacy: Vaccination of ducks with S DNA vaccines prevented the development of viremia following virus challenge. All ducks were challenged with a high-titer dose of DHBV virus. Preincubation of the virus with 5, 10, or, 20 μl of anti-S serum at 37°C for 1 hour prior to i.v. inoculation into 1-day-old ducklings completely prevented the development of viremia during a 4-week observation period in all of the ducks (Triyatni et al., 1998).

Ducks Response

  • Host Strain: Pekin
  • Vaccination Protocol: Four-week-old ducks received intramuscular (i.m.) injections of 100–300 μg of plasmid DNA diluted in NaCl 0.9%. The birds were injected in three sites (anterior quadriceps of both legs and breast), and booster doses were given 3 weeks later at the same sites (Thermet et al., 2003).
  • Challenge Protocol: Progeny ducklings received a high titre DHBV challenge and the viremia was followed by quantitative dot blot hybridisation for each animal during 17 days (Thermet et al., 2003).
  • Efficacy: Immunisation with a plasmid encoding the DHBV large (L) envelope protein (HBVgp2 pre-S1/pre-S2/S) induced a strong, specific, highly neutralising and long-lasting anti-preS humoral response in uninfected ducks. Importantly, maternal antibodies elicited by such DNA immunisation were vertically transmitted and protected progeny against viral challenge (Thermet et al., 2003).
References References
Triyatni et al., 1998: Triyatni M, Jilbert AR, Qiao M, Miller DS, Burrell CJ. Protective efficacy of DNA vaccines against duck hepatitis B virus infection. Journal of virology. 1998; 72(1); 84-94. [PubMed: 9420203].
Thermet et al., 2003: Thermet A, Rollier C, Zoulim F, Trepo C, Cova L. Progress in DNA vaccine for prophylaxis and therapy of hepatitis B. Vaccine. 2003; 21(7-8); 659-662. [PubMed: 12531335].