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Vaccine Detail

L. major DNA Vaccine encoding SP
Vaccine Information
  • Vaccine Name: L. major DNA Vaccine encoding SP
  • Target Pathogen: Leishmania major
  • Target Disease: Cutaneous leishmaniasis
  • Vaccine Ontology ID: VO_0004172
  • Type: DNA vaccine
  • Status: Research
  • SP gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pcDNA3.1 DNA vaccine plasmid
  • Immunization Route: Subcutanteous injection
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Immunization experiments were carried out twice at 3 weeks intervals in five groups of 10 BALB/c mice. Mice in group I (DNA/DNA) were immunized subcutaneously (s.c.) in the right footpad with 100 μg of pcDNA-sp plasmid, in the both prime and boost vaccination. Two control groups (IV and V) were studied; mice in group IV (vector only/CpG) were inoculated using 100 μg of pcDNA (vector without insert) in the first vaccination and the same adjuvant regimen (50 μg CpG ODN and 70 (v/v) Montanide 720) in the second vaccination. Mice in group V (PBS) received PBS alone (Rafati et al., 2006).
  • Challenge Protocol: Animals were then challenged 3 weeks after completion of the immunization protocol with 3 × 105 L. major MRHO/IR/75/ER metacyclic promastigotes that had been suspended in 50 μl PBS and injected into the left footpad (Rafati et al., 2006).
  • Efficacy: DNA/DNA strategy developed more effective protective responses and induced 81% reduction in L. major parasite load (Rafati et al., 2006).
References
Rafati et al., 2006: Rafati S, Ghaemimanesh F, Zahedifard F. Comparison of potential protection induced by three vaccination strategies (DNA/DNA, Protein/Protein and DNA/Protein) against Leishmania major infection using Signal Peptidase type I in BALB/c mice. Vaccine. 2006; 24(16); 3290-3297. [PubMed: 16481076].