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Vaccine Detail

Influenza virus DNA vaccine encoding HA from Influenza A virus (A/chicken/Henan/12/2004(H5N1))
Vaccine Information
  • Vaccine Name: Influenza virus DNA vaccine encoding HA from Influenza A virus (A/chicken/Henan/12/2004(H5N1))
  • Target Pathogen: Influenza virus
  • Target Disease: Influenza (flu)
  • Type: DNA vaccine
  • Status: Research
  • Antigen: Influenza A virus (A/chicken/Henan/12/2004(H5N1)) HA hemagglutinin
  • HA gene engineering:
    • Type: DNA vaccine construction
    • Description: Plasmid pCAGGSP7/HA was constructed by cloning the PCR product of HA from the A/Chicken/Henan/12/2004(H5N1) influenza virus strain into the plasmid expression vector pCAGGSP7 (Chen et al., 2009).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid:
    • DNA vaccine plasmid name:
    • DNA vaccine plasmid VO ID: VO_0005000
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunized with plasmid DNA dissolved in 30 μl of Tris–EDTA buffer at a dosage of 50 μg by injection into the quadriceps muscles. After injection in the right quadriceps muscle, a pair of electrode needles with 5 mm apart was inserted into the muscle to cover the DNA injection sites and electric pulses were delivered using an electric pulse generator (Electro Square Porator T830 M; BTX, San Diego, CA). Three pulses of 100 V each, followed by three pulses of the opposite polarity, were delivered to each injection site at a rate of one pulse per second. Each pulse lasted for 50 ms. A booster was given 2 weeks after the first immunization. The non-immunized mice were set up as controls (Chen et al., 2009).
  • Challenge Protocol: Two weeks after the last immunization, the mice were anesthetized and challenged with 20 μl of the viral suspension containing 5LD50 influenza virus A/Chicken/Henan/12/2004(H5N1) or A/PR/8/34 (H1N1) by intranasal drip (Chen et al., 2009).
  • Efficacy: Plasmids carrying the HA, NA, NP, M1, and M2 genes, respectively, of the avian influenza virus (AIV) A/Chicken/Henan/12/2004(H5N1) strain were administered to mice, and the mice were challenged with the homologous virus later. The mice immunized with HA plasmid once and the NA plasmid twice survived 100% (Chen et al., 2009).
References
Chen et al., 2009: Chen Q, Kuang H, Wang H, Fang F, Yang Z, Zhang Z, Zhang X, Chen Z. Comparing the ability of a series of viral protein-expressing plasmid DNAs to protect against H5N1 influenza virus. Virus genes. 2009; 38(1); 30-38. [PubMed: 19067149].