• Vaccine Name: Influenza virus DNA vaccine encoding HA from Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1))
• Target Pathogen: Influenza virus
• Target Disease: Influenza (flu)
• Vaccine Ontology ID: VO_0004020
• Type: DNA vaccine
• Status: Research
• Antigen: Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1)) HA hemagglutinin
• HA from Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1)) gene engineering:
  • Type: DNA vaccine construction
  • Description: Plasmid encoding HA (A/Thailand/1(KAN-1)/2004, GenBank AY555150) was synthesized using human-preferred codons and constructed in a CMV/R backbone by GeneArt (Regensburg, Germany) (Rao et al., 2010).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005004
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: BALB/c mice (10 animals for each group of HA alone, HA+NP, and HA+NP+M2; and 5 animals for the NP alone and vector control) were immunized three times with a total of 15 µg plasmid DNA in 100 µl of PBS (pH 7.4) intramuscularly at 0, 3, and 6 weeks. For the single HA or NP plasmid group, each group received 5 µg DNA for each plasmid with 10 µg of control vector as filler DNA (total 15 µg) (Rao et al., 2010).
• Challenge Protocol: Viral challenge experiments were performed 3 weeks after the last immunization. All challenged animals were exposed under CO2 anesthesia to an intranasal viral inoculum of 100 LD50 of A/Vietnam/1203/2004 virus. Body weight and survival were monitored for 21 days (Rao et al., 2010).
• Efficacy: All animals in the control and NP alone groups died within 6 days after viral challenge, whereas animals immunized with HA alone, HA+NP and HA+NP+M2 showed survival rates of 100, 90 and 70%, respectively (Rao et al., 2010).