• Vaccine Name: L. amazonensis DNA vaccine encoding GP46
• Target Pathogen: Leishmania amazonensis
• Target Disease: Leishmaniasis
• Vaccine Ontology ID: VO_0011354
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: mouse
• Antigen: L. amazonensis gp46
• Gp46 gene engineering:
  • Type: DNA vaccine construction
  • Description: cDNAs encoding L. m. amazonensis gp46 antigen was subcloned into the VR1012 plasmid (Dumonteil et al., 2000)
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000334
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Susceptible BALB/c mice were immunised with two i.m. injections of 100 microg of plasmid DNA (Dumonteil et al., 2000).
• Challenge Protocol: Mice were challenged by the injection of 4 x 10^6 L. m. mexicana parasites in the foot pad to evaluate protection (Dumonteil et al., 2000).
• Efficacy: Measurement of lesion size indicated that mice immunised with VR012-GP46 were partially protected against infection (Dumonteil et al., 2000).