• Vaccine Name: Phleum pratense Allergy Phl p 12 Subunit Vaccine
• Target Pathogen: Allergy
• Target Disease: Allergy
• Vaccine Ontology ID: VO_0011374
• Type: Subunit vaccine
• Status: Research
• Antigen: A recombinant protein comprised of the C terminus of Phl p 12 at its N terminus and the Phl p 12 N terminus at its C terminus, Phl p 12-rs, was expressed in Escherichia coli and purified (Westritschnig et al., 2007).
• PRO2 gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000127
• Immunization Route: subcutaneous injection

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Mice were immunized s.c. with either 10 µg of rPhl p 12, 10 µg of rPhl p 12-rs, or a equimolar mixture of the five peptides (2 µg of each peptide) adsorbed to aluminum hydroxide (Westritschnig et al., 2007).
• Immune Response: rPhl p 12-rs induced IgG1 reactivity to rPhl p 12, rBet v 2, rCor a 2, and rDau c 4 that was of comparable magnitude as the reactivity induced by rPhl p 12 (Westritschnig et al., 2007).
• Efficacy: rPhl p 12-rs and the Phl p 12-derived peptides induced lower allergenic immune responses to the wild-type allergen than rPhl p 12 upon immunization of the mice and thus exhibited reduced in vivo allergenicity (Westritschnig et al., 2007).