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Vaccine Detail

S. typhi DnaJ Protein Vaccine
Vaccine Information
  • Vaccine Name: S. typhi DnaJ Protein Vaccine
  • Target Pathogen: Salmonella spp.
  • Target Disease: Salmonellosis
  • Vaccine Ontology ID: VO_0004182
  • Type: Subunit vaccine
  • Status: Research
  • DnaJ gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: Freunds emulsified oil adjuvant
    • Adjuvant name: Freunds emulsified oil adjuvant
    • VO adjuvant ID: VO_0000133
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunised on day 0 by injecting 40 μg DnaJ/mouse emulsified in Freund's complete adjuvant (100 μl) i.p. Subsequent booster injections were given by injecting 30 μg DnaJ/mouse emulsified in Freund's incomplete adjuvant i.p. on 7th and 28th days. The control mice received adjuvant alone (Sagi et al., 2006).
  • Challenge Protocol: After 15 days of last immunisation, i.e. 43rd day the mice were challenged with 10 MLD (1 × 105 cells) of S. Typhimurium i.p. The animals were observed daily for 30 days for morbidity and mortality (Sagi et al., 2006).
  • Efficacy: Immunisation of mice with DnaJ derived from Salmonella enterica subsp. enterica serovar Typhi str. CT18 was found to provide 70% protection against lethal challenge by S. typhimurium indicating the possible use of DnaJ as vaccine candidate against typhoid (Sagi et al., 2006).
References
Sagi et al., 2006: Sagi SS, Paliwal P, Bansal A, Mishra C, Khan N, Mustoori SR, Ilavazhagan G, Sawhney RC, Banerjee PK. Studies on immunogenicity and protective efficacy of DnaJ of Salmonella Typhi against lethal infection by Salmonella Typhimurium in mice. Vaccine. 2006; 24(49-50); 7135-7141. [PubMed: 16887241].