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Vaccine Detail

S. typhi GroEL Protein Vaccine
Vaccine Information
  • Vaccine Name: S. typhi GroEL Protein Vaccine
  • Target Pathogen: Salmonella spp.
  • Target Disease: Salmonellosis
  • Vaccine Ontology ID: VO_0004183
  • Type: Subunit vaccine
  • Status: Research
  • GroEL gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: complete Freunds adjuvant
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Mice each were immunized i.m. with 10 μg of GroEL/mouse either alone or in combination with alum or CFA on 0, 7th, and 28th day as described earlier. As negative control, group of 10 mice was injected with endotoxin free water mixed with alum or CFA (Bansal et al., 2010).
  • Challenge Protocol: Two weeks after the last booster, all the groups were challenged with a lethal dose of 1 × 108 CFU/ml/mouse S. typhi Ty2 intraperitoneally (i.p.). The mice were observed daily for 30 days for any morbidity or mortality (Bansal et al., 2010).
  • Efficacy: Immunization of mice with GroEL + CFA provided 80% protection against lethal challenge by S. typhi in mice (Bansal et al., 2010).
References
Bansal et al., 2010: Bansal A, Paliwal PK, Sagi SS, Sairam M. Effect of adjuvants on immune response and protective immunity elicited by recombinant Hsp60 (GroEL) of Salmonella typhi against S. typhi infection. Molecular and cellular biochemistry. 2010; 337(1-2); 213-221. [PubMed: 19851830].