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Vaccine Detail

Salmonella DNA vaccine encoding SopB Protein
Vaccine Information
  • Vaccine Name: Salmonella DNA vaccine encoding SopB Protein
  • Target Pathogen: Salmonella spp.
  • Target Disease: Salmonellosis
  • Vaccine Ontology ID: VO_0004185
  • Type: DNA vaccine
  • Status: Research
  • sopB gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pCI-TPA DNA vaccine plasmid
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Six- to eight-week-old female BALB/c mice were immunized via i.p. route with 1 ml of PBS containing 103 CFU of ΔpmrG-HM-D strain per mouse and 10 μg of DNA vaccine plasmid in 50 μl volume of PBS in cohort of five mice. Booster doses were given on 7th and 14th day. Placebo control mice were injected with 103 CFU of ΔpmrG-HM-D strain per mouse and 10 μg of empty pCI-TPA vector (Nagarajan et al., 2009).
  • Challenge Protocol: 21 days after immunization, mice were challenged with 107 CFU/mouse of WT Salmonella orally (Nagarajan et al., 2009).
  • Efficacy: SopB DNA vaccine immunization reduced the bacterial burden of organs by about 5-fold on day 4 and day 8 after challenge with virulent Salmonella and proved to be a more efficient vaccination strategy than live attenuated bacteria alone (Nagarajan et al., 2009).
References
Nagarajan et al., 2009: Nagarajan AG, Balasundaram SV, Janice J, Karnam G, Eswarappa SM, Chakravortty D. SopB of Salmonella enterica serovar Typhimurium is a potential DNA vaccine candidate in conjugation with live attenuated bacteria. Vaccine. 2009; 27(21); 2804-2811. [PubMed: 19428891].