• Vaccine Name: L. monocytogenes DNA Vaccine encoding hly
• Target Pathogen: Listeria monocytogenes
• Target Disease: Listeriosis
• Vaccine Ontology ID: VO_0004031
• Type: DNA vaccine
• Status: Research
• Antigen: The hly gene of L. monocytogenes
• Hly gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000234
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: For immunization with the plasmid constructs, 6-to 8-wk-old mice received the first of a series of three i.m. immunizations (via the tibialis anterior muscles) at 3- to 4-wk intervals with ~100–125 µg of plasmid DNA in 50 µl of normal saline. Normal control mice received either no immunization or were immunized with either 0.2 ml of PBS (i.v.) or 50 µl of saline (i.m.) (Cornell et al., 1999).
• Challenge Protocol: Briefly, groups of normal or immunized BALB/c mice received an i.v. injection with ~2 LD50 (~20,000 CFU) of L. monocytogenes in 0.2 ml of PBS, at 3–4 wk following the final immunization with plasmid DNA. Control groups consisted of normal (nonimmunized) mice and mice previously immunized (4–12 wk earlier) with a sublethal injection of viable L. monocytogenes (Cornell et al., 1999).
• Efficacy: DNA immunization of mice provides protection against challenge with viable L. monocytogenes (Cornell et al., 1999).