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Vaccine Detail

P. aeruginosa DNA Vaccine encoding OprF
Vaccine Information
  • Vaccine Name: P. aeruginosa DNA Vaccine encoding OprF
  • Target Pathogen: Pseudomonas aeruginosa
  • Target Disease: Pseudomonas aeruginosa infection
  • Vaccine Ontology ID: VO_0004189
  • Type: DNA vaccine
  • Status: Research
  • Antigen: The pVR1020/oprF plasmid.
  • OprF gene engineering:
    • Type: DNA vaccine construction
    • Description: The 977-bp oprF gene was cloned from P. aeruginosa PAO1 genomic DNA into the eukaryotic expression plasmid pVR1020 (Price et al., 2001).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pVR1020 DNA vaccine plasmid
  • Immunization Route: abdomen gene gun
Host Response

Mouse Response

  • Host Strain: Pathogen Free ICR
  • Vaccination Protocol: At 14-day intervals, groups of 30 mice were inoculated a total of three times in the abdomen via biolistic particle injection (Helios Gene Gun kit; Bio-Rad, Richmond, Calif.) on days 0, 14, and 28 with 2 μg of either pVR1020 (control) or pVR1020/oprF, which was used to coat 1-μm-diameter gold beads (Price et al., 2001).
  • Challenge Protocol: Two weeks after the final immunization, the mice were challenged with agar beads containing the P. aeruginosa FD immunotype 4 strain. The mice were first anesthetized with an intraperitoneal injection of sodium pentobarbital and then inoculated via a tracheal incision with 50 μl of an agar bead slurry encasing approximately 7 × 102 CFU of P. aeruginosa (Price et al., 2001).
  • Efficacy: A significant reduction in the presence of severe macroscopic lesions, as well as in the number of bacteria present in the lungs, was seen in immunized mice (Price et al., 2001).
  • Description: The immunoprotective potential of the pVR1020/oprF vaccine was tested in a mouse model of chronic pulmonary infection (Price et al., 2001).
References
Price et al., 2001: Price BM, Galloway DR, Baker NR, Gilleland LB, Staczek J, Gilleland HE Jr. Protection against Pseudomonas aeruginosa chronic lung infection in mice by genetic immunization against outer membrane protein F (OprF) of P. aeruginosa. Infection and immunity. 2001; 69(5); 3510-3515. [PubMed: 11292786].