• Vaccine Name: P. aeruginosa DNA Vaccine encoding OprF
• Target Pathogen: Pseudomonas aeruginosa
• Target Disease: Pseudomonas aeruginosa infection
• Vaccine Ontology ID: VO_0004189
• Type: DNA vaccine
• Status: Research
• Antigen: The pVR1020/oprF plasmid.
• OprF gene engineering:
  • Type: DNA vaccine construction
  • Description: The 977-bp oprF gene was cloned from P. aeruginosa PAO1 genomic DNA into the eukaryotic expression plasmid pVR1020 (Price et al., 2001).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005060
• Immunization Route: abdomen gene gun

Mouse Response

• Host Strain: Pathogen Free ICR
• Vaccination Protocol: At 14-day intervals, groups of 30 mice were inoculated a total of three times in the abdomen via biolistic particle injection (Helios Gene Gun kit; Bio-Rad, Richmond, Calif.) on days 0, 14, and 28 with 2 μg of either pVR1020 (control) or pVR1020/oprF, which was used to coat 1-μm-diameter gold beads (Price et al., 2001).
• Challenge Protocol: Two weeks after the final immunization, the mice were challenged with agar beads containing the P. aeruginosa FD immunotype 4 strain. The mice were first anesthetized with an intraperitoneal injection of sodium pentobarbital and then inoculated via a tracheal incision with 50 μl of an agar bead slurry encasing approximately 7 × 10^2 CFU of P. aeruginosa (Price et al., 2001).
• Efficacy: A significant reduction in the presence of severe macroscopic lesions, as well as in the number of bacteria present in the lungs, was seen in immunized mice (Price et al., 2001).
• Description: The immunoprotective potential of the pVR1020/oprF vaccine was tested in a mouse model of chronic pulmonary infection (Price et al., 2001).