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Vaccine Detail

P. aeruginosa OprI Protein Vaccine
Vaccine Information
  • Vaccine Name: P. aeruginosa OprI Protein Vaccine
  • Target Pathogen: Pseudomonas aeruginosa
  • Target Disease: Pseudomonas aeruginosa infection
  • Vaccine Ontology ID: VO_0004190
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: Recombinant OprI protein
  • OprI gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: aluminum hydroxide vaccine adjuvant
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Female BALB/c mice, 12 to 16 weeks old, received 100 μl (27 ,ug) of OprI suspended in 100 μl of 1.5% Al(OH)3 intraperitoneally on days 0, 14, 35, and 63. Controls received Al(OH)3 only (Finke et al., 1990).
  • Challenge Protocol: mice were challenged intraperitoneally 10 days after the last immunization with 200 μl of a P. aeruginosa serogroup 6 suspension. Mice vaccinated with recombinant OprI received 6 x 106 to 1 x 108 living organisms each. (Finke et al., 1990).
  • Efficacy: Recombinant OprI protein was used to immunize mice, and was found to be protective against a challenge with a four- to fivefold 50% lethal dose of P. aeruginosa (Finke et al., 1990).
References
Finke et al., 1990: Finke M, DuchĂȘne M, Eckhardt A, Domdey H, von Specht BU. Protection against experimental Pseudomonas aeruginosa infection by recombinant P. aeruginosa lipoprotein I expressed in Escherichia coli. Infection and immunity. 1990; 58(7); 2241-2244. [PubMed: 2114360].