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Vaccine Detail

P. aeruginosa PcrV Protein Vaccine
Vaccine Information
  • Vaccine Name: P. aeruginosa PcrV Protein Vaccine
  • Target Pathogen: Pseudomonas aeruginosa
  • Target Disease: Pseudomonas aeruginosa infection
  • Vaccine Ontology ID: VO_0004191
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: PcrV
  • PcrV gene engineering:
    • Type: Recombinant protein preparation
    • Description: PcrV was produced as a lipopolysaccharide-free histidine-tagged infusion protein in pET16b and was purified by nickel chromatography (Holder et al., 2001).
    • Detailed Gene Information: Click Here.
  • Adjuvant: incomplete Freunds adjuvant
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Host Strain: CF-1
  • Vaccination Protocol: On day 0, groups of 10 female CF-1 mice weighing 22 to 25 g were immunized intramuscularly in the hind leg (10 μg of immunogen in 0.1 ml of incomplete Freund's adjuvant), followed by a booster dose (10 μg in saline) without adjuvant on day 14 (Holder et al., 2001).
  • Challenge Protocol: Using the burned mouse model, mice were challenged by one of three strains of P. aeruginosa (Holder et al., 2001).
  • Efficacy: Burned Pseudomonas aeruginosa-infected mice immunized against PcrV, a type III virulence system translocating protein, showed significantly enhanced survival compared to controls (Holder et al., 2001).
References
Holder et al., 2001: Holder IA, Neely AN, Frank DW. PcrV immunization enhances survival of burned Pseudomonas aeruginosa-infected mice. Infection and immunity. 2001; 69(9); 5908-5910. [PubMed: 11500471].