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Vaccine Detail

S. aureus CNA Protein Vaccine
Vaccine Information
  • Vaccine Name: S. aureus CNA Protein Vaccine
  • Target Pathogen: Staphylococcus aureus
  • Target Disease: Staph infections
  • Vaccine Ontology ID: VO_0004048
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: a recombinant fragment of CNA protein (Nilsson et al., 1998).
  • cna gene engineering:
    • Type: Recombinant protein preparation
    • Description: CNA fragments were expressed in E. coli and then purified (Nilsson et al., 1998).
    • Detailed Gene Information: Click Here.
  • Adjuvant: complete Freunds adjuvant
  • Immunization Route: subcutaneous injection
Host Response

Mouse Response

  • Host Strain: NMRI
  • Vaccination Protocol: Purified recombinant collagen adhesin fragments M17, M31, M55, and BSA (Sigma Chemical Co.) were dissolved in PBS and emulsified 1:1 in Freund’s complete adjuvant 200 μl of the emulsion containing 100 μg of protein was injected subcutaneously (s.c.) on day -31. Booster immunizations (s.c.; 100 μg protein in PBS) were performed 15 and 24 d after the initial vaccination (Nilsson et al., 1998).
  • Challenge Protocol: After the final boost, mice were inoculated intravenously (i.v.) with S. aureus (Nilsson et al., 1998).
  • Efficacy: At 14 d after inoculation, mortality in the collagen adhesin-vaccinated group was only 13%, compared with 87% in the control antigen immunized group (Nilsson et al., 1998).
References
Nilsson et al., 1998: Nilsson IM, Patti JM, Bremell T, Höök M, Tarkowski A. Vaccination with a recombinant fragment of collagen adhesin provides protection against Staphylococcus aureus-mediated septic death. The Journal of clinical investigation. 1998; 101(12); 2640-2649. [PubMed: 9637697].